CN110583261B - Method for planting Sasa sagittifolia by using biological fertilizer - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
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Abstract
The invention discloses a method for planting Sagittaria sagittifolia by utilizing a biological fertilizer, which comprises the following steps: applying biofertilizer and lime to the land for cutting the Sagittaria sagittifolia per mu, deep ploughing, applying biochar particles per mu, and deep ploughing again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges; selecting mother bamboos, cutting off branches from the connecting positions of the branches and the trunk, and soaking the branches into nutrient solution to culture and take roots; when cuttage is carried out, firstly, pit holes are dug on ridges, bamboo branches are transplanted after rooting, the bamboo branches are inserted into the pit holes, then, soil is covered on the bamboo branches, the bamboo branches are compressed and fixed, and then, nutrient solution is used for irrigating the roots; and performing conventional field management after cuttage, including timely watering, weeding and topdressing. According to the method for planting the Sagittaria sagittifolia by using the biological fertilizer, the planted Sagittifoliae is high in survival rate, low in pest and disease damage incidence rate and good in growth condition, the Sagittaria sagittifolia is high in fiber length-width ratio, appropriate in curling index and twisting index, low in lignin content and high in cellulose content, and the Sagittaria sagittifolia is used as a raw material for papermaking and has obvious advantages.
Description
Technical Field
The invention belongs to the field of arrowhead planting, and particularly relates to a method for planting arrowhead by using a biological fertilizer.
Background
With the development of economy and society, the awareness of environmental protection is gradually improved, and in the face of the problems of gradually severe environmental pollution, land desertification and the like, it is imperative to use other resources to replace wood as a paper making raw material. The effective utilization of non-wood fiber resources has great influence on environmental protection, forest resource maintenance, the reduction of dependence of the paper industry on wood and the like. The bamboo plant has fast growing speed and strong adaptability, the fiber length of the bamboo plant is slightly lower than that of wood but longer than that of grass plant, and the strength of paper is in direct proportion to the length of fiber, so the bamboo plant can be used as an excellent raw material for papermaking; bamboo is an important non-wood renewable resource, has the characteristics of high growth speed, high yield, wide application, high economic value and the like, has high comprehensive fiber content which can reach about 70 percent and is higher than reed, wheat straw and the like, and is a good papermaking raw material. Bamboo used for pulping and papermaking is mainly divided into two major categories, namely thin-wall category and thick-wall category; among them, Sagittaria sagittifolia belongs to the thin-wall class, and Phyllostachys pubescens belongs to the thick-wall class. At present, researches on bamboo pulping and papermaking mainly focus on improvement and optimization of pulping processes, analysis of bamboo structures, papermaking performances and the like of different bamboo species, and researches on optimizing and improving the yield of bamboo and improving the fiber quality of bamboo are rarely reported. Meanwhile, the existing fertilizer for the Sagittaria sagittifolia can not effectively improve the yield and the fiber quality of the Sagittaria sagittifolia.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for planting Sagittaria sagittifolia using a biofertilizer, comprising the steps of:
step one, applying 1200-1500 kg of biofertilizer and 80-100 kg of lime to each mu of land for cutting arrowroots, deeply ploughing the soil for at least 35cm, then applying 100-150 kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 1-2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that at least 6 leaves of the remaining parts of the branches are required to be ensured, and then soaking the branches in nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the depth of the pits is 18-25 cm, transplanting when the bamboo branches grow to 10-15 cm, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 80-100 mL of each bamboo branch;
step four, performing conventional field management after cuttage, including timely watering and weeding, performing topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on the side, close to the upward slope, applying 0.05-0.08 kg of fertilizer to each seedling, and covering with soil; the weeding and top dressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the top dressing amount is increased to 200-300 g of biological fertilizer per plant.
Preferably, the biological fertilizer comprises the following components in parts by weight:
40-50 parts of bamboo powder, 3-5 parts of composite biological bacteria, 5-8 parts of polyacrylamide, 15-18 parts of modified medical stone powder, 5-8 parts of chitosan, 20-25 parts of livestock and poultry manure, 3-8 parts of humic acid, 3-5 parts of sphacelainide lactone, 6-12 parts of fishbone powder, 3-6 parts of manganese carbonate, 8-12 parts of xylitol residues, 10-15 parts of urea and 3-5 parts of cobalt sulfate.
Preferably, the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, modified medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water which is 2-5 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation vat, adding the compound biological bacteria, and fermenting for 3-7 days at the temperature of 30-60 ℃;
and step III, drying the fermented material at 40-80 ℃, adding manganese carbonate, cobalt sulfate and sphacelolide, and performing ball milling and mixing to obtain the biological fertilizer.
Preferably, the process of ball milling and mixing comprises the following steps: adding the materials into a ball milling tank, adding ball milling balls and ball milling liquid, placing the ball milling balls and the ball milling liquid on a planetary ball mill, carrying out wet ball milling on the mixed materials for 3-5 hours, and then drying to obtain uniformly mixed raw materials; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, ball milling at a rotating speed of 300-350 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 10-12: 1.
Preferably, the composite biological bacteria is a mixture of more than two of bacillus subtilis, bacillus laterosporus, bacillus megaterium, EM bacteria and bacillus mucilaginosus.
Preferably, the preparation method of the modified medical stone powder comprises the following steps: adding 10-20 parts by weight of medical stone powder into a supercritical device, soaking for 60-90 min in a supercritical acetone-water system at the temperature of 350-370 ℃ and the pressure of 10-14 MPa, filtering and drying; the volume ratio of acetone to water in the supercritical acetone-water system is 3: 1; and ball-milling and mixing the dried product and rare earth salt to obtain the modified medical stone powder.
Preferably, the process of ball milling and mixing is as follows: adding the dried product, rare earth salt and stainless steel grinding balls into a ball-milling tank; introducing liquid nitrogen into the ball milling tank, completely immersing the dried product, the rare earth salt and the stainless steel ball milling into the liquid nitrogen at the temperature of-150-185 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; carrying out ball milling for 5-8 hours after keeping the temperature for 30-60 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, and placing for 12-24 hours; the weight ratio of the dried product to the rare earth salt is 3-5: 1; the weight ratio of the dried product to the stainless steel grinding ball is 1: 15-25.
Preferably, the rare earth salt is any one of acrylic acid rare earth salt, oleic acid rare earth salt, fumaric acid rare earth salt and p-vinyl benzene sulfonic acid rare earth salt.
Preferably, the biochar particles are any one of peanut shell carbon particles, straw carbon particles and walnut shell carbon particles.
Preferably, the nutrient solution comprises the following components in parts by weight: 30-50 parts of potassium nitrate, 10-15 parts of ammonium nitrate, 10-15 parts of diammonium phosphate, 5-10 parts of magnesium sulfate, 5-8 parts of urea, 1-3 parts of folic acid, 0.3-0.5 part of caffeic acid, 0.1-0.3 part of copper glycinate, 3-5 parts of tea polyphenol, 1-3 parts of asparagine, 0.3-0.5 part of xanthan gum, 1-3 parts of furfuryl methyl fumarate, 0.5-1.5 parts of cyclodextrin and 150-300 parts of water.
The invention at least comprises the following beneficial effects: according to the method for planting the Sasa albo-marginata by using the biological fertilizer, the planted Sasa albo-marginata is high in survival rate, low in pest and disease damage incidence rate and good in growth condition, the Sasa albo-marginata is high in fiber length-width ratio, appropriate in curling index and twisting index, low in lignin content and high in cellulose content, and the Sasa albo-marginata is used as a raw material for papermaking and is obvious in advantages.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
The specific implementation mode is as follows:
the present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
It should be noted that, in the cultivation method of fennel of the present invention, the experimental methods described in the following embodiments, unless otherwise specified, are all conventional methods, including but not limited to weeding, thinning, irrigating, harvesting, etc.; the reagents and materials are commercially available, unless otherwise specified.
Example 1:
a method for planting Sasa sagittifolia by using a biological fertilizer comprises the following steps:
step one, applying 1500kg of biofertilizer and 100kg of lime to each mu of land for cutting the Sagittaria sagittifolia, deeply ploughing the soil for at least 35cm, then applying 150kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that the rest parts of the branches are at least 6, and then soaking the branches in a nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the depth of the pits is 18cm, transplanting when the bamboo branches grow to 15cm in root, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 100mL of each bamboo branch; wherein the cuttage time is 4 months in the year;
step four, performing conventional field management after cuttage, including timely watering and weeding, topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on one side, applying 0.08kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 300g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
50 parts of bamboo powder, 5 parts of composite biological bacteria, 8 parts of polyacrylamide, 18 parts of medical stone powder, 8 parts of chitosan, 25 parts of livestock and poultry manure, 8 parts of humic acid, 5 parts of sphacelactone, 12 parts of fishbone powder, 6 parts of manganese carbonate, 12 parts of xylitol residue, 15 parts of urea and 5 parts of cobalt sulfate; the composite biological bacteria are a mixture of bacillus subtilis and EM bacteria in a weight ratio of 1: 1; the biological carbon particles are peanut shell carbon particles;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water which is 5 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation barrel, adding the composite biological bacteria, and fermenting for 7 days at 45 ℃;
step III, drying the fermented material at 80 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, then adding into a ball milling tank, adding ball milling balls and ball milling liquid, placing on a planetary ball mill, carrying out wet ball milling on the mixed material for 5 hours, and then drying to obtain the biological fertilizer; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, ball milling at 350 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 10: 1;
the nutrient solution comprises the following components in parts by weight: 30 parts of potassium nitrate, 15 parts of ammonium nitrate, 10 parts of diammonium phosphate, 5 parts of magnesium sulfate, 8 parts of urea, 3 parts of folic acid, 0.5 part of caffeic acid, 0.3 part of copper glycinate, 5 parts of tea polyphenol, 3 parts of asparagine, 0.5 part of xanthan gum, 3 parts of furfuryl alcohol methyl fumarate, 1.5 parts of cyclodextrin and 150 parts of water.
Example 2:
a method for planting Sasa sagittifolia by using a biological fertilizer comprises the following steps:
step one, applying 1200kg of biofertilizer and 80kg of lime to each mu of land for cutting arrowroots, deeply ploughing the soil for at least 35cm, then applying 100kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that the rest parts of the branches are at least 6, and then soaking the branches in a nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the pit depth is 25cm, transplanting when the bamboo branches grow to 10cm in root, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 100mL of each bamboo branch; wherein the cuttage time is 4 months in the year;
step four, performing conventional field management after cuttage, including timely watering and weeding, topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on the side, close to the upward slope, applying 0.05kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 200g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
40 parts of bamboo powder, 3 parts of composite biological bacteria, 5 parts of polyacrylamide, 15 parts of medical stone powder, 5 parts of chitosan, 20 parts of livestock and poultry manure, 3 parts of humic acid, 3 parts of sphacela lactone, 6 parts of fishbone powder, 3 parts of manganese carbonate, 8 parts of xylitol residue, 10 parts of urea and 3 parts of cobalt sulfate; the composite biological bacteria are a mixture of bacillus megaterium and EM bacteria in a weight ratio of 1: 1; the biological carbon particles are walnut shell carbon particles;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water 2 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation barrel, adding the composite biological bacteria, and fermenting for 7 days at the temperature of 30 ℃;
step III, drying the fermented material at 80 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, then adding into a ball milling tank, adding ball milling balls and ball milling liquid, placing on a planetary ball mill, carrying out wet ball milling on the mixed material for 5 hours, and then drying to obtain the biological fertilizer; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, the ball milling rotating speed is 300 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 12: 1;
the nutrient solution comprises the following components in parts by weight: 50 parts of potassium nitrate, 10 parts of ammonium nitrate, 15 parts of diammonium phosphate, 10 parts of magnesium sulfate, 5 parts of urea, 1 part of folic acid, 0.3 part of caffeic acid, 0.1 part of copper glycinate, 3 parts of tea polyphenol, 1 part of asparagine, 0.3 part of xanthan gum, 1 part of furfuryl alcohol methyl fumarate, 0.5 part of cyclodextrin and 300 parts of water.
Example 3:
a method for planting Sasa sagittifolia by using a biological fertilizer comprises the following steps:
step one, applying 1300kg of biofertilizer and 90kg of lime to each mu of land for cutting the Sagittaria sagittifolia, deeply ploughing the soil for at least 35cm, then applying 120kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that the rest parts of the branches are at least 6, and then soaking the branches in a nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the depth of the pits is 20cm, transplanting when the bamboo branches grow to 12cm, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 90mL of each bamboo branch; wherein the cuttage time is 4 months in the year;
step four, performing conventional field management after cuttage, including timely watering and weeding, topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on the side, close to the upward slope, applying 0.05kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 200g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
45 parts of bamboo powder, 4 parts of composite biological bacteria, 6 parts of polyacrylamide, 16 parts of medical stone powder, 6 parts of chitosan, 23 parts of livestock and poultry manure, 6 parts of humic acid, 4 parts of sphacelactone, 10 parts of fishbone powder, 5 parts of manganese carbonate, 10 parts of xylitol residue, 12 parts of urea and 4 parts of cobalt sulfate; the composite biological bacteria are a mixture of bacillus mucilaginosus and EM bacteria in a weight ratio of 1: 1; the biological carbon particles are straw carbon particles;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water in an amount which is 4 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation barrel, adding the composite biological bacteria, and fermenting for 5 days at 45 ℃;
step III, drying the fermented material at 60 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, then adding into a ball milling tank, adding ball milling balls and ball milling liquid, placing on a planetary ball mill, carrying out wet ball milling on the mixed material for 5 hours, and then drying to obtain the biological fertilizer; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, the ball milling speed is 320 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 11: 1;
the nutrient solution comprises the following components in parts by weight: 40 parts of potassium nitrate, 12 parts of ammonium nitrate, 12 parts of diammonium phosphate, 8 parts of magnesium sulfate, 6 parts of urea, 2 parts of folic acid, 0.4 part of caffeic acid, 0.2 part of copper glycinate, 4 parts of tea polyphenol, 2 parts of asparagine, 0.4 part of xanthan gum, 2 parts of furfuryl alcohol methyl fumarate, 1 part of cyclodextrin and 200 parts of water.
Example 4:
a method for planting Sasa sagittifolia by using a biological fertilizer comprises the following steps:
step one, applying 1500kg of biofertilizer and 100kg of lime to each mu of land for cutting the Sagittaria sagittifolia, deeply ploughing the soil for at least 35cm, then applying 150kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that the rest parts of the branches are at least 6, and then soaking the branches in a nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the depth of the pits is 18cm, transplanting when the bamboo branches grow to 15cm in root, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 100mL of each bamboo branch; wherein the cuttage time is 4 months in the year;
step four, performing conventional field management after cuttage, including timely watering and weeding, topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on one side, applying 0.08kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 300g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
50 parts of bamboo powder, 5 parts of composite biological bacteria, 8 parts of polyacrylamide, 18 parts of modified medical stone powder, 8 parts of chitosan, 25 parts of livestock and poultry manure, 8 parts of humic acid, 5 parts of sphacelactone, 12 parts of fishbone powder, 6 parts of manganese carbonate, 12 parts of xylitol residue, 15 parts of urea and 5 parts of cobalt sulfate; the composite biological bacteria are a mixture of bacillus subtilis and EM bacteria in a weight ratio of 1: 1; the biological carbon particles are peanut shell carbon particles;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, modified medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water which is 5 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation barrel, adding the composite biological bacteria, and fermenting for 7 days at 45 ℃;
step III, drying the fermented material at 80 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, then adding into a ball milling tank, adding ball milling balls and ball milling liquid, placing on a planetary ball mill, carrying out wet ball milling on the mixed material for 5 hours, and then drying to obtain the biological fertilizer; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, ball milling at 350 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 10: 1;
the preparation method of the modified medical stone powder comprises the following steps: adding 20 parts by weight of medical stone powder into a supercritical device, soaking for 90min in a supercritical acetone-water system with the temperature of 370 ℃ and the pressure of 14MPa, filtering and drying; the volume ratio of acetone to water in the supercritical acetone-water system is 3: 1; ball-milling and mixing the dried product and rare earth salt to obtain modified medical stone powder; the ball milling and mixing process comprises the following steps: adding the dried product, rare earth salt and stainless steel grinding balls into a ball-milling tank; introducing liquid nitrogen into the ball milling tank, completely immersing the dried product, the rare earth salt and the stainless steel ball milling into the liquid nitrogen at the temperature of-175 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; ball milling is started after the constant temperature is kept for 60min, and the ball milling is carried out for 8 hours; after the ball milling is finished, transferring the ball milling tank into a vacuum glove box, and standing for 24 hours; the weight ratio of the dried product to the rare earth salt is 5: 1; the weight ratio of the dried product to the stainless steel grinding ball is 1: 15; the rare earth salt is lanthanum acrylate;
the nutrient solution comprises the following components in parts by weight: 30 parts of potassium nitrate, 15 parts of ammonium nitrate, 10 parts of diammonium phosphate, 5 parts of magnesium sulfate, 8 parts of urea, 3 parts of folic acid, 0.5 part of caffeic acid, 0.3 part of copper glycinate, 5 parts of tea polyphenol, 3 parts of asparagine, 0.5 part of xanthan gum, 3 parts of furfuryl alcohol methyl fumarate, 1.5 parts of cyclodextrin and 150 parts of water.
Example 5:
a method for planting Sasa sagittifolia by using a biological fertilizer comprises the following steps:
step one, applying 1200kg of biofertilizer and 80kg of lime to each mu of land for cutting arrowroots, deeply ploughing the soil for at least 35cm, then applying 100kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that the rest parts of the branches are at least 6, and then soaking the branches in a nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the pit depth is 25cm, transplanting when the bamboo branches grow to 10cm in root, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 100mL of each bamboo branch; wherein the cuttage time is 4 months in the year;
step four, performing conventional field management after cuttage, including timely watering and weeding, topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on the side, close to the upward slope, applying 0.05kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 200g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
40 parts of bamboo powder, 3 parts of composite biological bacteria, 5 parts of polyacrylamide, 15 parts of medical stone powder, 5 parts of chitosan, 20 parts of livestock and poultry manure, 3 parts of humic acid, 3 parts of sphacela lactone, 6 parts of fishbone powder, 3 parts of manganese carbonate, 8 parts of xylitol residue, 10 parts of urea and 3 parts of cobalt sulfate; the composite biological bacteria are a mixture of bacillus megaterium and EM bacteria in a weight ratio of 1: 1; the biological carbon particles are walnut shell carbon particles;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water 2 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation barrel, adding the composite biological bacteria, and fermenting for 7 days at the temperature of 30 ℃;
step III, drying the fermented material at 80 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, then adding into a ball milling tank, adding ball milling balls and ball milling liquid, placing on a planetary ball mill, carrying out wet ball milling on the mixed material for 5 hours, and then drying to obtain the biological fertilizer; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, the ball milling rotating speed is 300 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 12: 1;
the preparation method of the modified medical stone powder comprises the following steps: adding 10 parts of medical stone powder into a supercritical device, soaking for 60min in a supercritical acetone-water system with the temperature of 350 ℃ and the pressure of 10MPa, filtering and drying; the volume ratio of acetone to water in the supercritical acetone-water system is 3: 1; and ball-milling and mixing the dried product and rare earth salt to obtain the modified medical stone powder.
Preferably, the process of ball milling and mixing is as follows: adding the dried product, rare earth salt and stainless steel grinding balls into a ball-milling tank; introducing liquid nitrogen into the ball milling tank, completely immersing the dried product, the rare earth salt and the stainless steel ball milling into the liquid nitrogen at the temperature of 185 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; ball milling is started after the constant temperature is kept for 60min, and the ball milling is carried out for 5 hours; after the ball milling is finished, transferring the ball milling tank into a vacuum glove box, and standing for 12 hours; the weight ratio of the dried product to the rare earth salt is 3: 1; the weight ratio of the dried product to the stainless steel grinding ball is 1: 15; the rare earth salt is lanthanum oleate;
the nutrient solution comprises the following components in parts by weight: 50 parts of potassium nitrate, 10 parts of ammonium nitrate, 15 parts of diammonium phosphate, 10 parts of magnesium sulfate, 5 parts of urea, 1 part of folic acid, 0.3 part of caffeic acid, 0.1 part of copper glycinate, 3 parts of tea polyphenol, 1 part of asparagine, 0.3 part of xanthan gum, 1 part of furfuryl alcohol methyl fumarate, 0.5 part of cyclodextrin and 300 parts of water.
Example 6:
a method for planting Sasa sagittifolia by using a biological fertilizer comprises the following steps:
step one, applying 1300kg of biofertilizer and 90kg of lime to each mu of land for cutting the Sagittaria sagittifolia, deeply ploughing the soil for at least 35cm, then applying 120kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges;
selecting 2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that the rest parts of the branches are at least 6, and then soaking the branches in a nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the depth of the pits is 20cm, transplanting when the bamboo branches grow to 12cm, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 90mL of each bamboo branch; wherein the cuttage time is 4 months in the year;
step four, performing conventional field management after cuttage, including timely watering and weeding, topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on the side, close to the upward slope, applying 0.05kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 200g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
45 parts of bamboo powder, 4 parts of composite biological bacteria, 6 parts of polyacrylamide, 16 parts of medical stone powder, 6 parts of chitosan, 23 parts of livestock and poultry manure, 6 parts of humic acid, 4 parts of sphacelactone, 10 parts of fishbone powder, 5 parts of manganese carbonate, 10 parts of xylitol residue, 12 parts of urea and 4 parts of cobalt sulfate; the composite biological bacteria are a mixture of bacillus mucilaginosus and EM bacteria in a weight ratio of 1: 1; the biological carbon particles are straw carbon particles;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water in an amount which is 4 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation barrel, adding the composite biological bacteria, and fermenting for 5 days at 45 ℃;
step III, drying the fermented material at 60 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, then adding into a ball milling tank, adding ball milling balls and ball milling liquid, placing on a planetary ball mill, carrying out wet ball milling on the mixed material for 5 hours, and then drying to obtain the biological fertilizer; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, the ball milling speed is 320 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 11: 1;
the preparation method of the modified medical stone powder comprises the following steps: adding 15 parts of medical stone powder into a supercritical device, soaking for 70min in a supercritical acetone-water system with the temperature of 360 ℃ and the pressure of 12MPa, filtering and drying; the volume ratio of acetone to water in the supercritical acetone-water system is 3: 1; ball-milling and mixing the dried product and rare earth salt to obtain modified medical stone powder; the ball milling and mixing process comprises the following steps: adding the dried product, rare earth salt and stainless steel grinding balls into a ball-milling tank; introducing liquid nitrogen into the ball milling tank, completely immersing the dried product, the rare earth salt and the stainless steel ball milling into the liquid nitrogen at-160 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; ball milling is started after the constant temperature is kept for 45min, and the ball milling is carried out for 6 hours; after the ball milling is finished, transferring the ball milling tank into a vacuum glove box, and standing for 18 hours; the weight ratio of the dried product to the rare earth salt is 4: 1; the weight ratio of the dried product to the stainless steel grinding ball is 1: 20; the rare earth salt is lanthanum oleate;
the nutrient solution comprises the following components in parts by weight: 40 parts of potassium nitrate, 12 parts of ammonium nitrate, 12 parts of diammonium phosphate, 8 parts of magnesium sulfate, 6 parts of urea, 2 parts of folic acid, 0.4 part of caffeic acid, 0.2 part of copper glycinate, 4 parts of tea polyphenol, 2 parts of asparagine, 0.4 part of xanthan gum, 2 parts of furfuryl alcohol methyl fumarate, 1 part of cyclodextrin and 200 parts of water.
Planting 200 Cizhu plants in the resource garden of southwest university of science and technology by the method of examples 1-6 in the same manner, observing survival rate, incidence rate of plant diseases and insect pests and growth conditions of the Cizhu plants, and recording in time as shown in Table 1;
TABLE 1
Planting 200 Ci bamboo plants in the resource garden of southwest university of science and technology by the method of examples 1-6 in the same way, and performing stalk fiber morphology determination on the Ci bamboo plants in the end of the second year after planting; the determination method comprises the following steps: selecting central stems of Sagittaria sagittifolia, cutting a part of the selected stems into the size of matchsticks, placing the matchsticks into 25mL centrifuge tubes, placing 3-4 centrifugal tubes into each centrifuge tube, immersing a sample in a separation solution prepared by mixing 10% nitric acid and 10% chromic acid in equal proportion, placing the sample at room temperature for 2-3d, replacing the separation solution once a day, placing the separated sample in a plate, washing the sample with pure water until the sample is colorless, and then determining Fiber forms (Fiber length, width, length-width ratio and the like), wherein the determination method is carried out according to the operation specification of a Fiber Quality Analyzer (code LDA02) high-precision Fiber form Analyzer. The method comprises the following specific steps:
the main power switch of the instrument is turned on, and the valve of the air pump is opened, so that the pressure in the tank reaches 0.4-0.6 Mpa. And starting the instrument, and displaying the program initial interface by the display normally. Distilled water is added into the instrument water storage tank until the distilled water is added to the water level scale mark, and then the water storage tank is ensured to have enough distilled water for testing in the subsequent measurement, and the pressure is ensured to be stabilized at 0.4-0.6 Mpa.
The first time the instrument is started, the instrument needs to be self-checked. And filling distilled water into a beaker of the instrument to 500ml of scale mark, placing the beaker on a beaker support, selecting an 'inspection' button, and performing subsequent measurement after the self-inspection of the instrument is finished.
Putting the cleaned and separated sample into a sample injector, adding a proper amount of distilled water, uniformly scattering the sample, transferring the sample into a beaker of the instrument, and adding distilled water to dilute the sample to 500 ml. The beaker is placed on a support, a 'measurement' button is selected, the name of the sample is recorded, parameters are set according to a setting guide, the default is generally selected, and 5000 fibers are selected according to the number of the fibers. Select "Start" button and begin measuring sample fiber morphology. The measurement should ensure that the fiber frequency does not exceed 60 fibers/s, otherwise, the error of the test data is large. The measurement was repeated 3 times for each sample.
After the sample test is finished, selecting a 'Result' button to display a test Result, storing the Result as a document, placing an empty beaker on a beaker support, selecting a 'Purge' button to clean an instrument test unit, and testing a next sample after the cleaning is finished.
After all samples were tested, an empty beaker was placed on the rack and the "Purge" button was selected until the remaining distilled water in the tank was completely drained. And (4) evacuating air in the air storage tank until the numerical value of the pressure gauge is zero. The instrument power switch is turned off. The main power switch is turned off. The results are shown in table 2:
200 Cisa bambusicola are planted in the same way in the resource garden of southwest university of science and technology by adopting the methods of examples 1-6, and the stem cellulose and lignin of the Cisa bambusicola are measured in the end of the second year after planting:
selecting central stem of Sagittaria sagittifolia, taking dried Sagittaria sagittifolia stem to be tested to constant weight, selecting a screen with the aperture of 0.2mm, crushing a sample by using a crusher, and analyzing the content of cellulose and lignin by referring to the steps of an operation instruction of a Fibertec TM 1020 type cellulose tester of FOSS company. And obtaining the cellulose content and the lignin content of the sample to be detected by an acid washing fiber (ADF) method and an acid washing lignin (ADL) method, wherein each sample is biologically repeated for 3 times, and each group of samples runs a blank control. The method comprises the following specific steps:
0.5g of the pulverized sample was weighed out and recorded as W0And placed in a crucible dried beforehand, and a blank was secured for each batch as a control.
The crucible was placed in the cold leach unit to ensure the instrumentation valve had closed. Adding 25ml of acetone into each crucible, leaching for 5min, opening a valve to a 'vacuum' level, opening a vacuum pump, and completely filtering the acetone. This step was repeated three times.
And transferring the crucible to a crucible frame, and transferring the crucible to a hot leaching unit after the acetone is completely volatilized. To each leaching column 100ml of preheated Acidic Detergent (ADS) was added. And opening a condensed water switch of the instrument. And (5) turning on a heating controller, and adjusting to 4 steps for heating. And when all the reagents in the leaching columns start to boil, adjusting the heating controller to control the boiling speed, keeping the boiling state and timing for 10 min. And then closing the heater and the condensed water, placing the valve in a 'vacuum' gear, and opening the vacuum pump to finish suction filtration. Preheated distilled water was added to the leaching column and the sample was rinsed until it was neutral. The crucible was transferred to a crucible stand, cooled and transferred to a cold leaching unit, rinsed 3 times with acetone. After all the organic solvent is completely volatilized, the crucible is placed in a 120 ℃ oven to be dried for 2 hours, and then the crucible is driedThe crucible was transferred to a desiccator to cool to room temperature, weighed and recorded as W1Blank sample W1`。
And (3) moving the crucible to a cold leaching unit, adding 72% sulfuric acid into the crucible, soaking for 3 hours at room temperature, then opening a vacuum pump, placing a valve at a 'vacuum' gear to complete suction filtration, and finally washing with distilled water to be neutral. Then the crucible is placed in a 120 ℃ oven to be dried for 2h, the crucible is transferred to a drier to be cooled to room temperature, and the crucible is weighed and recorded as W2Blank sample W2`。
Finally adding the crucible, transferring the crucible into a muffle furnace to perform ashing for 3h at 525 ℃, transferring the crucible into a dryer to be cooled to room temperature, weighing and recording as W3Blank sample W3`。
And calculating the content of cellulose and lignin in the sample according to a formula.
The statistical results of the measurements are shown in table 2:
TABLE 2
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the examples shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (3)
1. A method for planting Sagittaria sagittifolia by using a biological fertilizer is characterized by comprising the following steps:
step one, applying 1200-1500 kg of biofertilizer and 80-100 kg of lime to each mu of land for cutting arrowroots, deeply ploughing the soil for at least 35cm, then applying 100-150 kg of charcoal particles to each mu of land, and deeply ploughing the soil for at least 35cm again; ridging to form drainage ditches, and tamping soil and the drainage ditches of the ridges; the biochar particles are any one of peanut shell carbon particles, straw carbon particles and walnut shell carbon particles;
selecting 1-2-year-old strong bamboos without diseases and insect pests from the Sasa albo-marginata forest as mother bamboos, cutting off branches of the mother bamboos from a connecting position with a trunk, removing leaves within 0.5m away from a cut on the bamboos, ensuring that at least 6 leaves of the remaining parts of the branches are required to be ensured, and then soaking the branches in nutrient solution to culture and take roots;
digging pits on the ridges during cuttage, wherein the depth of the pits is 18-25 cm, transplanting when the bamboo branches grow to 10-15 cm, inserting the bamboo branches into the pits, covering soil to compress and fix the bamboo branches, irrigating the roots with nutrient solution, and irrigating 80-100 mL of each bamboo branch;
step four, performing conventional field management after cuttage, including timely watering and weeding, performing topdressing in the last ten days of the current year from 6 th month to 7 th month, wherein the topdressing fertilizer is a biological fertilizer, excavating a fertilizing hole with the depth of about 15cm from the bamboo seedling on the side, close to the upward slope, of the bamboo seedling on the side, close to the upward slope, applying 0.05-0.08 kg of fertilizer to each seedling, and covering with soil; weeding and topdressing in the second year are respectively arranged in 4-5 months and 7-8 months, and the topdressing amount is increased to 200-300 g of biological fertilizer per plant;
the biological fertilizer comprises the following components in parts by weight:
40-50 parts of bamboo powder, 3-5 parts of composite biological bacteria, 5-8 parts of polyacrylamide, 15-18 parts of modified medical stone powder, 5-8 parts of chitosan, 20-25 parts of livestock and poultry manure, 3-8 parts of humic acid, 3-5 parts of sphacelactone, 6-12 parts of fishbone powder, 3-6 parts of manganese carbonate, 8-12 parts of xylitol residues, 10-15 parts of urea and 3-5 parts of cobalt sulfate;
the preparation method of the modified medical stone powder comprises the following steps: adding 10-20 parts by weight of medical stone powder into a supercritical device, soaking for 60-90 min in a supercritical acetone-water system at the temperature of 350-370 ℃ and the pressure of 10-14 MPa, filtering and drying; the volume ratio of acetone to water in the supercritical acetone-water system is 3: 1; ball-milling and mixing the dried product and rare earth salt to obtain modified medical stone powder;
the ball milling and mixing process comprises the following steps: adding the dried product, rare earth salt and stainless steel grinding balls into a ball-milling tank; introducing liquid nitrogen into the ball milling tank, completely immersing the dried product, the rare earth salt and the stainless steel ball milling into the liquid nitrogen at the temperature of-150-185 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; carrying out ball milling for 5-8 hours after keeping the temperature for 30-60 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, and placing for 12-24 hours; the weight ratio of the dried product to the rare earth salt is 3-5: 1; the weight ratio of the dried product to the stainless steel grinding ball is 1: 15-25;
the rare earth salt is any one of acrylic acid rare earth salt, oleic acid rare earth salt, fumaric acid rare earth salt and p-vinyl benzene sulfonic acid rare earth salt;
the preparation method of the biological fertilizer comprises the following steps:
step I, mixing bamboo powder, polyacrylamide, modified medical stone powder, chitosan, livestock and poultry manure, humic acid, fishbone powder, xylitol residues and urea in parts by weight, adding water which is 2-5 times of the weight of the mixture, and uniformly stirring;
step II, adding the uniformly stirred mixture obtained in the step I into a fermentation vat, adding the compound biological bacteria, and fermenting for 3-7 days at the temperature of 30-60 ℃;
step III, drying the fermented material at 40-80 ℃, adding manganese carbonate, cobalt sulfate and sphacelactone, and performing ball milling and mixing to obtain a biological fertilizer;
the process of ball milling and mixing comprises the following steps: adding the materials into a ball milling tank, adding ball milling balls and ball milling liquid, placing the ball milling balls and the ball milling liquid on a planetary ball mill, carrying out wet ball milling on the mixed materials for 3-5 hours, and then drying to obtain uniformly mixed raw materials; the ball-milling ball adopts ceramic balls, the diameter of a big ball is about 8mm, the diameter of a small ball is about 5mm, and the mass ratio of the big ball to the small ball is 1: 4, the ball material ratio, namely the weight ratio of the ball grinding balls to the materials is 8: 1, ball milling at a rotating speed of 300-350 r/min; the ball milling liquid is water, and the weight ratio of the ball milling liquid to the materials is 10-12: 1.
2. The method for planting Sasa sagittifolia using a biofertilizer according to claim 1, wherein the complex biological bacteria is a mixture of two or more of Bacillus subtilis, Bacillus laterosporus, Bacillus megaterium, EM bacteria, and Bacillus mucilaginosus.
3. The method for planting Sagittaria sagittifolia with biofertilizer according to claim 1, wherein the nutrient solution comprises the following components in parts by weight: 30-50 parts of potassium nitrate, 10-15 parts of ammonium nitrate, 10-15 parts of diammonium phosphate, 5-10 parts of magnesium sulfate, 5-8 parts of urea, 1-3 parts of folic acid, 0.3-0.5 part of caffeic acid, 0.1-0.3 part of copper glycinate, 3-5 parts of tea polyphenol, 1-3 parts of asparagine, 0.3-0.5 part of xanthan gum, 1-3 parts of furfuryl methyl fumarate, 0.5-1.5 parts of cyclodextrin and 150-300 parts of water.
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