CN109953042A - A kind of inhibitor that acacia confusa is grown based on grignard evergreen chinquapin litter leaching liquor - Google Patents
A kind of inhibitor that acacia confusa is grown based on grignard evergreen chinquapin litter leaching liquor Download PDFInfo
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- CN109953042A CN109953042A CN201910349273.XA CN201910349273A CN109953042A CN 109953042 A CN109953042 A CN 109953042A CN 201910349273 A CN201910349273 A CN 201910349273A CN 109953042 A CN109953042 A CN 109953042A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The present invention relates to a kind of preparation methods of inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa, and by grignard evergreen chinquapin sample 3 times wash with distilled water, dries pulverizing is simultaneously sieved.It takes Litter-fall 1g after sieving to be put into 50 mL centrifuge tubes and 15 mL70% ethyl alcohol are added, is extracted 2 times, each 1h in 80 DEG C of water-baths, centrifuge, 8000 r min of setting setting are put into after cooling‑1Centrifugation 5 minutes, filtering extracting solution will merge twice, and add water to 50 mL.It places it on Rotary Evaporators after ethanol evaporation, then is settled to the stoste that 1:5 is made in 50 mL.The result shows that, leaching liquor when 1:5 concentration is most obvious for the inhibiting effect of acacia confusa tree species, and can maintain good vital sign by after inhibitor application acacia confusa, is a kind of good seedling inhibitor and without using chemicals, no pollution to the environment.
Description
Technical field
The invention belongs to plant growth inhibitor fields, and in particular to one kind is based on grignard evergreen chinquapin litter leaching liquor to Taiwan
The preparation method of the inhibitor of yearning between lovers growth.
Background technique
Rare or endangered species are as species resource, one of genetic resources and the main body of ecological environment, to the maintenance ecological balance
It is of great significance.The interplanting tree species for being suitable for rare or endangered species are built, its habitat water and soil conservation, soil improvement, disease is conducive to
Pest control and reduction fire etc..But Mixed species are improper to be will lead to main body tree species and repels to interplanting tree species, and generates evil
Sexual competition and murder by poisoning.Allelopathic Effect in Plants is widely present in mixed forest, is the important sources for causing this murder by poisoning, is mainly passed through
It causes the sprouting of Mixed effect seed, growth of seedling, blossom and bear fruit etc. to be affected to Environment release allelochemical, and then influence
Population foundation and update.Litter-fall acts on subject plant seed sprouting as one of allelochemical main source, leaching liquor
Significantly, such research is concerned in recent years: Yang Lixue is research shows that high concentration fallen leaves pine needle infusion sprouts juglans mandshurica seed
Hair and growth of seedling, which have, is obviously promoted effect, and as infusion concentration reduces, seed is sprouted and growth of seedling is on a declining curve.
Chen Juan etc. is research shows that influence of the moso bamboo leaching liquor to bitter sweet oak seedling height of seedling, ground diameter and chlorophyll relative content is totally in high concentration
Inhibit, the dual concentration effect that low concentration promotes.The newly equal Litter-fall leaf of carrying out of Chen Li is to Korean pine seeds sprouting and growth of seedling shadow
Ring research, the results showed that mixed pine forest litter leaching liquor is reduced with concentration, and inhibiting effect weakens, disappears or be changed into promotion.
As it can be seen that from internal association between allelopathic angle elaboration species, to explanation plant individual and inter-species Coupling effects and syndynamic
With fine directive function.
Grignard evergreen chinquapin (Castanopsis kawakamii) is the peculiar evergreen broad-leaved arbor of middle subtropical zone, lonely as the Tertiary Period
It is extremely precious to lose plant, is only sporadicly distributed on Fujian, Taiwan, Jiangxi, Guangdong and Guangxi and other places.The main collection of grignard evergreen chinquapin research at present
In in synecology, gap regeneration, wildwood and artificial forest Litter-fall and carbon base in soil nutrient pool research, grignard evergreen chinquapin wildwood
It is and mixed to castanopsis kanakamii symbiosis with artificial forest rhizosphere soil microorganism and its biochemical characteristic and Litter-fall-soil stoichiometry
Hand over tree species STUDY ON ALLELOPATHIC EFFECTS actually rare.Acacia confusa is as mixed forest common tree, existing southwest, the southeast each province torrid zone and Asia
There is cultivation in torrid areas.Its speed of growth is fast, and wide adaptability, biomass is big, and water storage moisturizing is strong, and interplanting advantage essentially consists in
Nitrogen content abundant in itself nitrogen fixation and Litter-fall, annual 1hm2 Litter-fall can provide 3500 kg litters according to surveying and determination,
500kg urea is equivalent to after decomposition.And grignard evergreen chinquapin nature reserve area total soil nitrogen and full phosphorus pole lack, and restrict grignard evergreen chinquapin seed and sprout
Hair and seedlings regeneration.To expand grignard evergreen chinquapin germ plasm resource, acacia confusa just becomes the mixed friendship of excellent forest of collection economy, ecological benefits
Tree species.As it can be seen that it is necessary to inquire into grignard evergreen chinquapin wildwood Litter-fall to acacia confusa with the presence or absence of allelopathic toxic action.For this purpose, with
Acacia confusa is research object, by measurement analysis various concentration grignard evergreen chinquapin litter leaching liquor to acacia confusa germination shadow
It rings, allelopathy of the analysis grignard evergreen chinquapin Litter-fall to acacia confusa seed growth, it is intended to interplant Taiwan phase for grignard evergreen chinquapin wildwood
Think to provide scientific basis.
It is generalized in the interplanting of grignard evergreen chinquapin artificial forest by the experience of grignard evergreen chinquapin interplanting acacia confusa tree species, then is generalized to other
Mature forest interplants acacia confusa tree species.One new thinking is proposed to the excellent situation for improving objective forest growth.Make to interplant tree species
Excellent nitrogen fixation can not only be played, and does not replace target tree species, there is equilibrium relations between tree species and tree species.
Summary of the invention
The purpose of the present invention is to provide a kind of inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of preparation method of the inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa, specifically includes following step
It is rapid:
(1) Litter-fall pre-processes: by grignard evergreen chinquapin Litter-fall sample 3 times wash with distilled water, the soil of removal surface attachment and corruption
Matter is grown, is crushed after 70 DEG C of drying, crosses 40 meshes；
(2) 1 extractions: taking step (1) pretreated Litter-fall, the ethyl alcohol of 70% v/v is added, after mixing, in 80 DEG C
1h is extracted in water-bath, after being cooled to room temperature, 8000rpm is centrifuged 5min, and filtrate is obtained by filtration；
(3) 2 extractions: the ethyl alcohol with 70% v/v of step (2) same volume is added into filter residue, after mixing, in 80 DEG C
1h is extracted in water-bath, after being cooled to room temperature, 8000rpm is centrifuged 5min, and filtrate is obtained by filtration；
(3) it rotates: merging the filtrate that step (2) and (3) extract twice, add water to be settled to the stoste that 1:5 is made in 50mL, be placed on rotation
It steams on evaporimeter after revolving to ethyl alcohol evaporating completely, then is settled to 50 milliliters, is i.e. acquisition inhibitor leaching liquor stoste；
(4) dilute: by step (3) stoste add by volume distilled water dilute 3 times, 7 times, 15 times, 31 times, 63 times, be made 1:20,
The leaching liquor of five kinds of various concentrations of 1:40,1:80,1:160,1:320.
Step (1) institute lattice Shu Shi evergreen chinquapin Litter-fall sample is that leaf, shell, branch, skin 1:1:1:1 in mass ratio that grignard evergreen chinquapin withers and falls are mixed
It closes.
The mass volume ratio of the ethyl alcohol of step (2) the pretreated Litter-fall and 70% v/v is 1:15.
A kind of inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa is in inhibiting acacia confusa growth
Application, specifically includes the following steps:
(1) acacia confusa seed of the same size is chosen, sterilizes 30min with the potassium permanganate of 0.5wt%, then be washed with distilled water 3
It is secondary, it is homogeneously disposed in the culture dish for putting the filter paper that haves three layers, each culture dish uniformly places 100 seeds；
(2) the inhibitor leaching liquor after 15mL dilution is added in each culture dish, is cultivated, 25 DEG C of the photoperiod, 12 in the incubator
H, 15 DEG C, 12 h of the dark period, 50 nmol cm of light intensity-1•s-1, relative humidity 75 % ~ 80 %；Supplement 4mL respective concentration daily
Leaching liquor is wet with filter paper；
(3) with the same day for disposing germination be the 1st day, the 3rd day after start to observe and record data, continuous observation 15 days；Note daily
Chitting piece quantity is recorded, kind of 2 mm of skin is exposed as standard using radicle, germinative number is counted day by day, to germination peak period, from culture
Each 20 measurement radicle lengths of the consistent seed of radicle length are selected in ware and are recorded；When sprouting for three days on end without seed, germination terminates
Experimental record；When radicle growth amount is basically unchanged, radicle experiment terminates, and the germination percentage, germinating energy, germination for finally calculating seed refer to
Number, radicle growth situation.
The present invention has the advantages that
(1) present invention is using acacia confusa as research object, by measurement analysis various concentration grignard evergreen chinquapin litter leaching liquor to platform
Gulf yearning between lovers germination influences, and analysis grignard evergreen chinquapin Litter-fall is that grignard evergreen chinquapin is natural to the allelopathy of acacia confusa seed growth
Woods interplanting acacia confusa provides scientific basis.
(2) present invention is generalized in the interplanting of grignard evergreen chinquapin artificial forest by the experience of grignard evergreen chinquapin interplanting acacia confusa tree species, then
Other mature forests interplanting acacia confusa tree species are generalized to, a new thinking is proposed to the excellent situation for improving objective forest growth,
Make interplanting tree species that can not only play excellent nitrogen fixation, and do not replace target tree species, there is balances between tree species and tree species
Relationship.
(3) present invention is obvious for the inhibiting effect of acacia confusa tree species using grignard evergreen chinquapin litter leaching liquor, and can tie up
Good vital sign is held, is a kind of good seedling inhibitor and without using chemicals, no pollution to the environment.
Detailed description of the invention
Fig. 1 is influence of the various concentration litter leaching liquor to acacia confusa radicle length.
Specific embodiment
(1) materials and methods:
In September, 2016 is withered and fallen in Sanming Nature Reserve of Castanopsis kawakamii permanent sample plot collects Litter-fall in frame month by month, and points 4 kinds (leaf,
Shell, branch, skin), and count respectively wither and fall species, etc. mass ratioes be mixed into 1 kg.Experimental material is acacia confusa seed.Laboratory
Interior preparation 70%(v/v) ethyl alcohol, 0.5wt% potassium permanganate, water-bath, centrifuge, centrifuge tube, 50 mL volumetric flasks, rotary evaporation
Instrument, vernier caliper, culture dish and incubator are used for acacia confusa seed culture.
(2) prepared by leaching liquor:
By the Litter-fall sample taken back wash with distilled water 3 times with remove surface attachment soil and humus, 70 DEG C of ovendry powers
Broken and 40 meshes excessively.1 g of Litter-fall after sieving is taken to be put into 50 mL centrifuge tubes and 15 mL70%(v/v are added) ethyl alcohol, in 80 DEG C of water
1h is extracted in bath, centrifuge is put into after cooling, and setting 8000rpm is centrifuged 5 minutes, filtering；Repeat above-mentioned steps extraction one
It is secondary, extracting solution will merge twice, and add water to 50 mL(and do 3 repetitions).It places it on Rotary Evaporators after ethanol evaporation,
It is settled to the stoste that 1:5 is made in 50 mL again, is added without Litter-fall according to the above method as a control group, then by volume by stoste
Add distilled water to dilute 3 times, 7 times, 15 times, 31 times, 63 times, five kinds of various concentrations of 1:20,1:40,1:80,1:160,1:320 are made
Leaching liquor.It is stored in 4 DEG C of refrigerators stand-by.
(3) seed sprouts experiment:
Selected acacia confusa seed of the same size sterilizes 30 min with the potassium permanganate of 0.5 wt%, then is washed 3 times with distillation,
It is homogeneously disposed in the culture dish for putting the filter paper that haves three layers, each culture dish uniformly places 100 seeds, is added in each culture dish
Configured various concentration leaching liquor is control with distilled water, and totally 7 processing, 3 repetitions of each processing are trained in the incubator
It supports.Condition of culture are as follows: 25 DEG C of the photoperiod, 12 h, 15 DEG C, 12 h of the dark period, 50 nmol cm of light intensity-1•s-1, relative humidity
75 %~80 %.It supplements a small amount of respective concentration leaching liquor daily to moisten with filter paper, disposing the same day of germination is after the 1st day, the 3rd day
Start to observe and record data, continuous observation 15 days.Record chitting piece quantity daily, exposing kind of 2 mm of skin with radicle is mark
Standard counts germinative number day by day, and to germination peak period, the seed for selecting radicle length almost the same from various concentration culture dish is each
20 measurement radicle lengths simultaneously record.When sprouting for three days on end without seed, germination terminates experimental record；Radicle growth amount is substantially not
When change, radicle experiment terminates, and finally calculates germination percentage, germinating energy, the germination index, radicle growth situation of seed.
Germination percentage (%)=(sub- sum is planted experimentally in seed number/confession of germination) × 100%；
Seed number/confession that germinating energy (%)=per day germinative number normally germinates until reaching highest that day plant experimentally sub- sum ×
100%；
Germination index=∑ (Gt/Dt)
Wherein: Dt refers to that germination number of days, Gt refer to daily chitting piece number corresponding with Dt and germination number of days
Result of study is shown in Table 1, distilled water group acacia confusa seed germination rate 63%, with 1:5,1:20,1:40,1:80,1:160,
When the leaching liquor processing of 1:320 various concentration, germination rate is respectively 60%, 57%, 60.5%, 63.5%, 66%, 64%, seed hair
Bud gesture distilled water group is 50%, leaching liquor group is followed successively by 13.5% by concentration from high to low, 46%, 46.5%, 52.5%, 54.5%,
51%, seed germination index distilled water group is 81.32, leaching liquor group is followed successively by 56.06 by concentration from high to low, 73.77,
75.98,84.26,88.61,81.46, such as Fig. 1, at the end of radicle is tested, the radicle length distilled water group of seed is 30.05mm,
Leaching liquor group is followed successively by 19.5mm, 25.54mm, 33.57mm, 33.54mm, 36.09mm, 33.00mm by concentration from high to low.
Influence of the 1 various concentration leaching liquor of table to acacia confusa germination
In conclusion leaching liquor when 1:5 concentration, most obvious for the inhibiting effect of acacia confusa tree species, and can maintain good
Vital sign, be a kind of good seedling inhibitor and without using chemicals, no pollution to the environment.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (4)
1. a kind of preparation method of the inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa, which is characterized in that
Specifically includes the following steps:
(1) Litter-fall pre-processes: by grignard evergreen chinquapin Litter-fall sample 3 times wash with distilled water, the soil of removal surface attachment and corruption
Matter is grown, is crushed after 70 DEG C of drying, crosses 40 meshes；
(2) 1 extractions: taking step (1) pretreated Litter-fall, the ethyl alcohol of 70% v/v is added, after mixing, in 80 DEG C
1h is extracted in water-bath, after being cooled to room temperature, 8000rpm is centrifuged 5min, and filtrate is obtained by filtration；
(3) 2 extractions: the ethyl alcohol with 70% v/v of step (2) same volume is added into filter residue, after mixing, in 80 DEG C
1h is extracted in water-bath, after being cooled to room temperature, 8000rpm is centrifuged 5min, and filtrate is obtained by filtration；
(3) it rotates: merging the filtrate that step (2) and (3) extract twice, add water to be settled to the stoste that 1:5 is made in 50mL, be placed on rotation
It steams on evaporimeter after revolving to ethyl alcohol evaporating completely, then is settled to 50 milliliters, is i.e. acquisition inhibitor leaching liquor stoste；
(4) dilute: by step (3) stoste add by volume distilled water dilute 3 times, 7 times, 15 times, 31 times, 63 times, be made 1:20,
The leaching liquor of five kinds of various concentrations of 1:40,1:80,1:160,1:320.
2. a kind of preparation of the inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa according to claim 1
Method, which is characterized in that step (1) institute lattice Shu Shi evergreen chinquapin Litter-fall sample be grignard evergreen chinquapin wither and fall leaf, shell, branch, skin in mass ratio
1:1:1:1 mixing.
3. a kind of preparation of the inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa according to claim 1
Method, which is characterized in that the mass volume ratio of the ethyl alcohol of step (2) the pretreated Litter-fall and 70% v/v is 1:15.
4. a kind of inhibitor grown based on grignard evergreen chinquapin litter leaching liquor to acacia confusa is in inhibiting acacia confusa growth
Using, which is characterized in that specifically includes the following steps:
(1) acacia confusa seed of the same size is chosen, sterilizes 30min with the potassium permanganate of 0.5wt%, then be washed with distilled water 3
It is secondary, it is homogeneously disposed in the culture dish for putting the filter paper that haves three layers, each culture dish uniformly places 100 seeds；
(2) the inhibitor leaching liquor after 15mL dilution is added in each culture dish, is cultivated, 25 DEG C of the photoperiod, 12 in the incubator
H, 15 DEG C, 12 h of the dark period, 50 nmol cm of light intensity-1•s-1, relative humidity 75 % ~ 80 %；Supplement 4mL respective concentration daily
Leaching liquor is wet with filter paper；
(3) with the same day for disposing germination be the 1st day, the 3rd day after start to observe and record data, continuous observation 15 days；Note daily
Chitting piece quantity is recorded, kind of 2 mm of skin is exposed as standard using radicle, germinative number is counted day by day, to germination peak period, from culture
Each 20 measurement radicle lengths of the consistent seed of radicle length are selected in ware and are recorded；When sprouting for three days on end without seed, germination terminates
Experimental record；When radicle growth amount is basically unchanged, radicle experiment terminates, and the germination percentage, germinating energy, germination for finally calculating seed refer to
Number, radicle growth situation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110558339A (en) * | 2019-10-21 | 2019-12-13 | 福建农林大学 | Inhibitor for masson pine growth based on Grignard tannin extract litter leaching solution |
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| CN110558339A (en) * | 2019-10-21 | 2019-12-13 | 福建农林大学 | Inhibitor for masson pine growth based on Grignard tannin extract litter leaching solution |
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