CN105659969A - Method for increasing seed germination rate of ammopiptanthus mongolicus through allelopathy of peganum harmala - Google Patents
Method for increasing seed germination rate of ammopiptanthus mongolicus through allelopathy of peganum harmala Download PDFInfo
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- CN105659969A CN105659969A CN201610063985.1A CN201610063985A CN105659969A CN 105659969 A CN105659969 A CN 105659969A CN 201610063985 A CN201610063985 A CN 201610063985A CN 105659969 A CN105659969 A CN 105659969A
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- Prior art keywords
- harmel
- cauline leaf
- seed
- ammopiptanthus mongolicus
- culture dish
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- 240000005523 Peganum harmala Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000586291 Ammopiptanthus mongolicus Species 0.000 title claims abstract description 22
- 230000007226 seed germination Effects 0.000 title claims abstract description 15
- 230000036531 allelopathy Effects 0.000 title claims abstract description 14
- 235000005126 Peganum harmala Nutrition 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000011218 seed culture Methods 0.000 claims abstract description 4
- 238000002386 leaching Methods 0.000 claims description 15
- 239000012153 distilled water Substances 0.000 claims description 9
- 238000001802 infusion Methods 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 7
- 230000012010 growth Effects 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000011149 active material Substances 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 239000003292 glue Substances 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims description 2
- 230000035784 germination Effects 0.000 abstract description 18
- 239000000126 substance Substances 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000003809 water extraction Methods 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000002137 ultrasound extraction Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 19
- 238000011160 research Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000035040 seed growth Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 235000003325 Ilex Nutrition 0.000 description 1
- 241000209035 Ilex Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
- 230000029602 competition with other organism Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention discloses a method for increasing the seed germination rate of ammopiptanthus mongolicus through allelopathy of peganum harmala. The method comprises steps as follows: 1, test material treatment: the peganum harmala is dried, crushed and sieved; 2, preparation of an extracting liquid: the extracting solution is prepared sequentially through water extraction, ultrasonic extraction, water extraction and centrifugation; 3, seed culture: a peganum harmala stem leaf water extracting solution is injected to a culture dish where ammopiptanthus mongolicus seeds are sown, the culture dish is covered with a lid and put in an intelligent manual climatic box for culture, the temperature is kept 25 DEG C in the daytime and 20 DEG C at night, the humidity is kept 75%, the illumination is performed for 12 h, and the light intensity is 8,000 LX. The method has the benefits as follows: (1) the cost is low; (2) the germination rate is high and ranges from 83% to 89% (the germination rate is 58% in the contrast group); (3) the germination speed is high, and the average germination index ranges from 61.5%-66% (the average germination index is 53% in the contrast group); (4) no chemicals are used, and the method causes no pollution to the environment.
Description
Technical field
The present invention relates to a kind of method that improves seed germination rate, be specifically related to one and utilize camelFluffy allelopathy improves the method for Ammopiptanthus mongolicus seed germination rate, belongs to breeding technical field.
Background technology
The allelopathy (Alelopathy) of plant refers in the ecosystem growing plants or itsThereby litter discharges particular chemicals by number of ways to environment to be promoted or suppresses selfOr the effect of contiguous plant seed germination and growth.
Between current plant, allelopathy has become the important scientific issues of ecological study, researchField mainly concentrates on the sides such as denizen invasion, the succession of community, genetic breeding and agriculture and forestry productionFace, and obtained the achievement of attracting attention; The territorial scope of research is mainly in coastal, temperate zone and coldBand area, grassland; Floristics is with crops, forest plants, flowers, herbage, medicinal material and waterRaw plant is main, and between China arid-desert area plant, the research of allelopathy rarely has report abroadRoad, but also do not cause the attention of domestic scholars.
Research shows in severe habitat, and it is dense that plant self tends to increase the release of metaboliteDegree and there are stronger allelopathic potentiality.
Therefore, the ermophyte in long term growth, awful weather habitat barren at soil is to be more worthThe allelopathic plant resources that must further investigate.
Summary of the invention
The object of the present invention is to provide one to utilize harmel allelopathy to improve Ammopiptanthus mongolicus seedThe method of germination rate, the method is except effectively improving the germination rate of Ammopiptanthus mongolicus seed, Hai NengtiHigh its speed of germination.
In order to realize above-mentioned target, the present invention adopts following technical scheme:
Utilize a method for harmel allelopathy raising Ammopiptanthus mongolicus seed germination rate, its featureBe, comprise the following steps:
One, for examination material processed:
(1) harmel plant won to cauline leaf, clean, air drying and stoving is to constant weight, pulverize,Sieving, it is stand-by to be placed on 4 DEG C of low temperature lower seals;
(2) select Ammopiptanthus mongolicus seed even, full, of the same size, carry out successively clear water punchingWash-warm water seed soaking-HgCl2Immersion-distilled water flushing, is then seeded in and is covered with double-deck filter paperStand-by in culture dish;
Two, leaching liquor preparation:
Harmel cauline leaf powder is placed in to distilled water, under room temperature, soaks, adopt afterwards ultrasonic wave to soakFormulation is extracted active material, lixiviate again at 40 DEG C, and afterwards by centrifugal after leaching liquor double filtration,Supernatant is harmel cauline leaf leaching liquor, adjusts the concentration of harmel cauline leaf leaching liquor at 5-15mg·ml-1In scope;
Three, seed culture:
Inject harmel cauline leaf infusion, culture dish to having sowed in the culture dish of Ammopiptanthus mongolicus seedAdd a cover to be placed in intelligent growth cabinet and cultivate, temperature is 25 DEG C of daytimes, 20 DEG C of nights,Humidity 75%, illumination 12h, light intensity 8000LX.
Aforesaid method, is characterized in that, in step 1, the bake out temperature of harmel is70-85℃。
Aforesaid method, is characterized in that, in step 1, the aperture of sieve is 1mm.
Aforesaid method, is characterized in that, in step 2, harmel cauline leaf powder at room temperatureImmersion duration is 48h.
Aforesaid method, is characterized in that, in the process of at room temperature soaking, shakes every 12hMoving 10min.
Aforesaid method, is characterized in that, in step 2, harmel cauline leaf powder is at 40 DEG CImmersion duration is 30min.
Aforesaid method, is characterized in that, in step 3, start cultivate after, every day glueDropper sops up liquid unnecessary in culture dish, and supplements the infusion of appropriate corresponding concentration.
Usefulness of the present invention is: (1) cost is low; (2) germination rate is high, at 83%-89%In scope (control group is 58%); (3) speed of germination is fast, and average germination index exists61.5%-66% (control group is 53%); (4) do not use chemicals, environmentally safe.
Detailed description of the invention
The present invention gives birth in imminent danger planting from the angle research plant stem-leaf infusion of Allelopathic Effect in Plants to sandThe impact of thing Ammopiptanthus mongolicus seed germination and growth of seedling, illustrates its allelopathy mechanism, is intended to visitStudy carefully the interaction relationship between harmel and other species, can more fully provide one simultaneouslyFrom external condition angle thinking Desert Regions Population Distribution Pattern, the interspecies competition in addition of plant growthThe method of all many-sided problems such as mechanism, succession of community rule.
The present invention not only contributes to enrich STUDY ON ALLELOPATHIC EFFECTS theory, and to instructing this district vegetationBuild significant.
Below in conjunction with specific embodiment, the present invention is done to concrete introduction.
The first step: for examination material processed
Testing harmel plant used and Ammopiptanthus mongolicus seed adopted respectively in September And October, 2015From Shiyanghe River downstream.
Harmel plant is taken back behind laboratory, won cauline leaf clean with distilled water, after dryingBe positioned at 80 DEG C and dry to constant weight, pulverize, sieve (aperture 1mm) is placed on 4 DEG C lowTemperature lower seal is stand-by.
Select Ammopiptanthus mongolicus seed even, full, of the same size, first seed is rinsed with clear waterTotally, then with 55-60 DEG C of warm water seed soaking 15min, then use 0.1% HgCl2Soak3min, rinses 5-6 time (to wash residual HgCl off repeatedly with distilled water2) after be seeded in and be covered with bilayerIn the culture dish of filter paper (culture dish and filter paper all do high-temperature disinfection treatment), every ware is put 50 kindsSon, stand-by.
Second step: leaching liquor preparation
Take pulverizing, harmel cauline leaf powder 20g after sieving, be placed in 500ml beaker, addEnter 200ml distilled water, with preservative film sealing and at lower 48h (this that soaks of room temperature (20-25 DEG C)During this time every 12h shake 10min), adopt afterwards ultrasonic-leaching method to extract active material,Lixiviate 30min at 40 DEG C, afterwards by after leaching liquor double filtration (first with quantitative filter paper filter,The filtering with microporous membrane that is 0.45um with filter unit again) centrifugal 15min under 6500r, instituteObtaining supernatant is that concentration is 100mgml-1Harmel cauline leaf lixiviate mother liquor, be placed in 4 DEG C of iceIn case, preserve.
When use, leaching liquor dilute be respectively 5,15,25,50mgml-1For subsequent use.
The 3rd step: seed culture
To having sowed the harmel cauline leaf that injects respectively variable concentrations in the culture dish of Ammopiptanthus mongolicus seedInfusion 10ml (first at room temperature places infusion a period of time before use, in order to avoid quenchingAmmopiptanthus mongolicus seed is produced to injury), contrast adds 10ml distilled water, every processing to arrange 3Repeat, culture dish is added a cover and is placed on (the RQX-400H Shanghai leap medical treatment of intelligent growth cabinetApparatus Co., Ltd) in cultivate, temperature is 25 DEG C of daytimes, 20 DEG C of nights, humidity 75%,Illumination 12h, light intensity 8000LX.
After starting to cultivate, sop up in culture dish unnecessary liquid with glue head dropper every day, and supplementThe infusion of 1ml corresponding concentration or distilled water, to keep leaching liquor concentration and moisture in culture dishSituation is constant.
Observational record every day germination number (radicle is broken through seed coat 1mm and is considered as sprouting) is also chosenExcept chitting piece, off-test when 3d no longer includes new seed germination continuously.
The 4th step: calculate germination percentage
Germination percentage (%)=(subnumber is planted experimentally in chitting piece number/confession) × 100%
Germination index GI=∑ (Gt/Dt)
In formula, GtRefer to the seed number of germinateing within t day; DtRefer to corresponding germination number of days.
In general, the immersion liquid of allelopathic vegetation water is sprouted recipient plant and is had showing of " low short height pressing down "Resemble, this phenomenon shows very outstanding in Ammopiptanthus mongolicus during Seed Germination:
When control treatment, Ammopiptanthus mongolicus seed germination rate is 58%, with 5,15,25,50mgml-1When the leaching liquor of variable concentrations is processed, its germination rate is respectively 89%, 83%, 71%, 44%,And the average germination index of seed compared with contrast also reduce with the rising of cauline leaf infusion concentration,Its value is respectively 53% (contrast), 66%, 61.5%, 43%, 23%.
This phenomenon explanation, the harmel cauline leaf infusion of low concentration not only can significantly improve sandThe seed germination rate of Chinese ilex, and also have significant quickening to do to the speed of Ammopiptanthus mongolicus seed germinationWith.
As can be seen here, method of the present invention not only can significantly improve the seed germination rate of Ammopiptanthus mongolicus,But also can accelerate significantly the speed of Ammopiptanthus mongolicus seed germination.
In addition, because the present invention has mainly used harmel leaching liquor, so breeding cost not onlyLow, and do not use chemicals, environmentally safe, has fabulous application value.
Because arid-desert area growth and development of plants and distribution are subject to multiple severe extraneous factor shadowRing, and biochemical substances is to affect the important factor that natural plant upgrades, people were for growth in the pastArid-desert area plant regeneration success or failure under severe habitat are often residing from seed and seedlingThe external condition such as illumination, moisture, nutrient is considered more, and to as upgrade success or failure potential because ofSon---underestimating of allelopathy. Allelopathy is prevalent between the kind of plant, plant in,With regard to desertification control field, rationally application will produce good effect. Therefore, pay attention toWith the research of carrying out ermophyte allelopathy, not only for explaining arid-desert area plant lifeMechanism and implement revegetation and there is directive function, and the closing of Dui Gai district agricultural and ecological safetyReason improvement and sustainable development are significant.
It should be noted that, above-described embodiment does not limit the present invention, all employings etc. in any formThe technical scheme obtaining with the mode of replacement or equivalent transformation, all drops on protection model of the present inventionIn enclosing.
Claims (7)
1. utilize a method for harmel allelopathy raising Ammopiptanthus mongolicus seed germination rate, its spyLevy and be, comprise the following steps:
One, for examination material processed:
(1) harmel plant won to cauline leaf, clean, air drying and stoving is to constant weight, pulverize,Sieving, it is stand-by to be placed on 4 DEG C of low temperature lower seals;
(2) select Ammopiptanthus mongolicus seed even, full, of the same size, carry out successively clear water punchingWash-warm water seed soaking-HgCl2Immersion-distilled water flushing, is then seeded in and is covered with double-deck filter paperStand-by in culture dish;
Two, leaching liquor preparation:
Harmel cauline leaf powder is placed in to distilled water, under room temperature, soaks, adopt afterwards ultrasonic wave to soakFormulation is extracted active material, lixiviate again at 40 DEG C, and afterwards by centrifugal after leaching liquor double filtration,Supernatant is harmel cauline leaf leaching liquor, adjusts the concentration of harmel cauline leaf leaching liquor at 5-15mg·ml-1In scope;
Three, seed culture:
Inject harmel cauline leaf infusion, culture dish to having sowed in the culture dish of Ammopiptanthus mongolicus seedAdd a cover to be placed in intelligent growth cabinet and cultivate, temperature is 25 DEG C of daytimes, 20 DEG C of nights,Humidity 75%, illumination 12h, light intensity 8000LX.
2. method according to claim 1, is characterized in that, in step 1, and camelFluffy bake out temperature is 70-85 DEG C.
3. method according to claim 1, is characterized in that, in step 1, and sieveAperture be 1mm.
4. method according to claim 1, is characterized in that, in step 2, and camelIt is 48h that fluffy cauline leaf powder at room temperature soaks duration.
5. method according to claim 4, is characterized in that, the mistake of at room temperature soakingCheng Zhong, every 12h shake 10min.
6. method according to claim 1, is characterized in that, in step 2, and camelIt is 30min that fluffy cauline leaf powder soaks duration at 40 DEG C.
7. method according to claim 1, is characterized in that, in step 3, startsAfter cultivation, sop up in culture dish unnecessary liquid with glue head dropper every day, and supplement appropriate correspondingThe infusion of concentration.
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|---|---|---|---|
| CN201610063985.1A CN105659969B (en) | 2016-01-29 | 2016-01-29 | A kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy |
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| CN201610063985.1A CN105659969B (en) | 2016-01-29 | 2016-01-29 | A kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613424A (en) * | 2017-01-18 | 2017-05-10 | 云南农业大学 | Micro-plant hydrotrope allelopathic potential evaluation bioassay method |
| CN106717220A (en) * | 2017-02-15 | 2017-05-31 | 兰州大学 | The method for regulating and controlling psilium succession with litter extract solution |
| CN107912455A (en) * | 2017-11-30 | 2018-04-17 | 融安县浪溪河谷金桔开发有限责任公司 | A kind of processing method for improving annual fleabane herb seed germination rate |
| CN109169120A (en) * | 2018-09-10 | 2019-01-11 | 天津农学院 | A kind of careless composite plant method of pulse family Qiao and its verification method based on allelopathy |
| CN109953043A (en) * | 2019-04-28 | 2019-07-02 | 福建农林大学 | It is a kind of based on grignard evergreen chinquapin litter leaching liquor to the inhibitor of companion species Growth of Chinese Fir |
| CN109953042A (en) * | 2019-04-28 | 2019-07-02 | 福建农林大学 | A kind of inhibitor that acacia confusa is grown based on grignard evergreen chinquapin litter leaching liquor |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613424A (en) * | 2017-01-18 | 2017-05-10 | 云南农业大学 | Micro-plant hydrotrope allelopathic potential evaluation bioassay method |
| CN106717220A (en) * | 2017-02-15 | 2017-05-31 | 兰州大学 | The method for regulating and controlling psilium succession with litter extract solution |
| CN107912455A (en) * | 2017-11-30 | 2018-04-17 | 融安县浪溪河谷金桔开发有限责任公司 | A kind of processing method for improving annual fleabane herb seed germination rate |
| CN109169120A (en) * | 2018-09-10 | 2019-01-11 | 天津农学院 | A kind of careless composite plant method of pulse family Qiao and its verification method based on allelopathy |
| CN109953043A (en) * | 2019-04-28 | 2019-07-02 | 福建农林大学 | It is a kind of based on grignard evergreen chinquapin litter leaching liquor to the inhibitor of companion species Growth of Chinese Fir |
| CN109953042A (en) * | 2019-04-28 | 2019-07-02 | 福建农林大学 | A kind of inhibitor that acacia confusa is grown based on grignard evergreen chinquapin litter leaching liquor |
| CN109953043B (en) * | 2019-04-28 | 2021-03-30 | 福建农林大学 | Inhibitor for growth of associated variety of Chinese fir based on Grignard tannin extract litter leaching liquor |
| CN109953042B (en) * | 2019-04-28 | 2021-03-30 | 福建农林大学 | Inhibitor for growth of acacia taiwan based on leaching liquor of castanopsis gracilis litter |
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| CN105659969B (en) | 2018-03-13 |
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