CN106613424A - Micro-plant hydrotrope allelopathic potential evaluation bioassay method - Google Patents
Micro-plant hydrotrope allelopathic potential evaluation bioassay method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a micro-plant hydrotrope allelopathic potential evaluation bioassay method. The method comprises the steps of using an overground part of donor plants with 20-30 days, cutting the overground part into 1-2 cm after surface sterilization, leaching the cut parts in sterile water at 4 DEG C for 24 h, conducting film filtering and sterilization, and obtaining plant water leaching liquor; conducting the same sterilization on receptor plant seeds and then sowing the receptor plant seeds into a petri dish which contains 6% of agal-agal, when roots are downwards perpendicularly cultivated till the length of the root is 0.5-1 cm, adding 5-10 microliter of the donor plant water leaching liquor, continuing cultivation for 2-3 days in a culture room at 25 or 28 DEG C for 12 hours light/dark culture and then taking out the donor plant water leaching liquor to measure amount of growth, comparing the amount of growth of the donor plants with that of the receptor plants which are not affected by the donor plants, and calculating influence conditions of plant root system secretions on the growth of the receptor plants to conduct allelopathic potential evaluation. The micro-plant hydrotrope allelopathic potential evaluation bioassay method has the advantages that only few donor plants are needed to extract water-soluble matter, there is no need to adjust osmotic pressure, bioactivity of plant water-soluble matter can be reflected while microbial interference can be controlled to the greatest extent, and the micro-plant hydrotrope allelopathic potential evaluation bioassay method is a simple, fast, economical and accurate bioassay method.
Description
Technical field
The present invention relates to a kind of micro plant hydrotrope allelopathic effects evaluate bioassay method, category biometric techniques neck
Domain.
Background technology
Interbiotic allelopathy is just recognized by the mankind from ancient times, and using with arrangement crop in agricultural production
Crop rotation.International allelopathic association (International Allelopathy Society) defines allelopathy within 1996
For：Bion is by the effect that other biological grows around it of Environment release compounds affect.Plant to environment is released
Put chemical substance organic and inorganic, its form includes：Root system secretion, escaping gas, leaching material etc..Allelopathy is made
With being a kind of chemical manifestations form that plant is adapted to environment, plant roots, stem, Ye Deng organ or tissues are to Environment release " allelopathic
Material " affects the effect of other plant, is also the form of expression of plant self-defense or anti-adversity ability.
This self-defense or anti-adversity ability using plant in the ecosystem, it is possible to reduce chemical pesticide makes
With, reduce production cost input, reduce environmental pressure, the excavation of allelopathic effects with using being a kind of resources conservation, environmental friendliness
Harmful organism controlling soil moist.Allelopathic Effect in Plants has become an important directions of current bioscience research, is also to promote
The important component part of agricultural sustainable development.Screening and evaluation Allelopathic Effect in Plants potentiality are the first of STUDY ON ALLELOPATHIC EFFECTS
Step, needs using simply, fast and accurately bioassay method carries out screening and assessment.Since 1985, scientist both domestic and external
Research reports the bioassay method of many Allelopathic potentials, and such as water planting is determined1, ladder method of testing2, International Rice research
Barnyard grass late sowing method3, Japanese Scientists agar root case method4And sandwich5, plant water extract method6、7, Australia
The agar of scientist co-cultures method8, root exudates method9Deng.Wherein sandwich, plant water extract method are with plant shoot
Point or underground part grade different tissues aqueous extract to for examination biological growth, development impact carry out allelopathic effects evaluation.Plant
The different tissues organ such as root, stem and leaf of thing be secondary metabolites synthesis significant points, the secondary metabolites/change of donor plant
Sense material is typically stored within plant cell, to exist with reference to state, is decomposed by biology enzyme or environment-stress and from plant
Privileged site for example root, stem etc. tissue discharge and produce biologically active.The measure knot of sandwich, plant water extract method
Fruit reflects the biologically active of the water-soluble substances contained by plant, also represents plant leached materials into after environment to other
The influence of plant., seed limited for seed source sprouts material irregular, that culture is difficult, plant water extract method
Very advantageous and irreplaceability, and by wide application allelopathic effects evaluate sod cultivation, and with it is easy to operate,
Quickly, the characteristics of can in high volume operating.However, when, material few for rare plant, quantity obtains limited, water extract
Method it is then infeasible, and if aseptic process to a large amount of plant leaching liquors, storage will increase experimentation cost, additionally, by
In the water extract osmotic potential difference of variable concentrations, opposite surveys result and produces impact10, therefore, existing plant water extract life
There is following defect and limitation in thing determination method：(1) it is difficult to obtain biomaterial allelopathic effects evaluation, (2) plant water extract
It is vulnerable to the technical problem of microbiological effect in the environment, (3) water extract variable concentrations osmotic potential grows shadow to recipient plant
Ring.
The present invention be directed to the needs of different Allelopathic Effect in Plants Potential Evaluation biologicall tests, according to plant seedling growth root
The characteristic that point absorbs, there is provided enough temperature, light, water conditions, excludes the interspecies competition of not kindred plant syntrophism, has both reacted plant
The biologically active of strain water-soluble substances controls to greatest extent microorganism interference again, by improving existing conventional biologicall test side
Method and the bioassay method invented.
Bibliography
1.Einhehelig,F.A.,G.R.Leather and L.Hobbs.1985.Use of Lemmaminor L.as
a bioassay in allelopathy.J.Chem.Ecol.11:65-72.
2.Liu,D.L.and J.V.Lovett.1993.Biologically active sencondary
metabolites of barleyⅠDeveloping techniques and accessing allelopathy in
barley.J.Chem.Ecol.19:2217-2230.
3.Navarez,D.and M.Olofsdotter.1996.Relay seeding technique for
screening allelopathic rice(Oryza sativa).Pages 1285-1290in Brow,H.et
al.eds.Proc.Second International Weed Control Congress,Copenhagen,Denmark.
4.Fujii,Y.1992.The potential biological control of paddy weeds with
allelopathy:allelopathic effect of some rice varieties.Pages 305-320in
Proc.Int.Symposium on Biological Control and Integrated Management of Paddy
and Aquatic Weeds in Asia.Tsukuba,Japan.National Agricultural Research
Center.
5.Fujii Y.,S.S.Parvez,M.Parvez,Y.O Hmae,O.Iida 2003Screening of
239medicinal plant species for allelopathic activity using the sandwich
method Weed Biology and Management,3(4)233-241.
6.Kim,K.U.,D.H.Shin,H.Y.Kim,I.J.Lee and M.Olofsdotter.1999.Evaluation
of allelopathic potential in rice germplasm.Korean J.of Weed Sci.9(2):1-9.
7.Ebana,K.,W.G.Yan,R.H.Dilday,H.Namai and K.Okuno.2001.Variation in
the allelopathic effect of rice with water soluble extracts.Agron.J.93:12–16.
8.Wu,H.,J.Pratley,D.Lemerle and T.Haig.2001.Allelopathy in wheat
(Triticum aestivum).Ann.Appl.Biol.139:1–9.
9th, Guo Yiqing, Jin Jixiong, bioassay method China of the journey in a kind of congruent secretions from plant roots allelopathic effects
Patent ZL 201010608481.6
The impact China of the osmotic potential on allelopathy bioassa of the 10th, Shao Hua, Peng Shaolin, relaxation etc. 2003
Ecological agriculture journal 11 (3)：36-37.
The content of the invention
It is an object of the invention to overcome the deficiency of prior art, and provide it is a kind of it is quick, reliable, can in high volume operate
Micro plant hydrotrope allelopathic effects evaluate bioassay method.
The micro plant hydrotrope allelopathic effects of the present invention evaluate bioassay method, are in the liquefaction of existing plant flooding
Improvement on the basis of sense Potential Evaluation bioassay method.Micro plant hydrotrope allelopathic effects after improvement evaluate biologicall test
Method, it is comprised the following steps that:
(1) prepared by culture medium
It is >=6% agar solution to prepare concentration in terms of mass fraction with water, by the agar solution for preparing 120 DEG C,
0.1MPa sterilizing 20min pour into stand-by in circular and square culture dish；
(2) culture of donor plant A and water extract prepare
Described donor plant A seeds are placed on culture dish and are placed in vernalization in 25 DEG C or 28 DEG C of culturing room after fully absorbing water
48 hours, in being then seeded in the little basin for being fitted into soil, it is placed in 25 DEG C or 28 DEG C, the chamber planting of 12 hours light dark conditions
20-30 days, clip aerial part was that 3-6% sodium hypochlorite carries out surface sterilization 5min with mass fraction, then clear with sterilized water
2-3 time is washed to tasteless, with the blotting paper of sterilizing excessive moisture is suctioned out, donor plant A scissors is cut into the segment of 1-2cm length,
It is put into sterile chamber, by donor plant A and mass volume ratio=1 of aqua sterilisa:5-10 adds sterilizing manhole cover, 4 DEG C of shaking tables
Extract 24 hours, with syringe leaching liquor is drawn, the aseptic plant of 20%-10% is obtained using 0.2 μm of sterilising filtration membrane filtration
Water extract, 4 DEG C of Refrigerator stores are standby；
(3) the surface of the seed sterilization and sprouting of recipient plant B
It with mass fraction is that 3-6% sodium hypochlorite enters to recipient plant B that the surface of the seed sterilization of described recipient plant B is
Row the surface of the seed is sterilized 10min, then extremely tasteless with sterile water wash 2-3 time, then recipient plant B is seeded in into above-mentioned 1 accurate
In the culture medium of standby circular culture dish, aseptic condition sprout standby to showing money or valuables one carries unintentionally；
(4) sowing of recipient plant B and culture
The middle part at the square culture dish back side in above-mentioned steps 1 is rule with ruler, will be sprouted in step 3 to receiving for showing money or valuables one carries unintentionally
Body plant B is sowed into culture dish, is rule by culture dish back and neatly sow, per ware 15-20 grains, by the culture dish after capping
The culture for being placed on 25 DEG C or 28 DEG C is indoor, light dark culture in 12 hours 3-5 days, and root is cultivated downward vertically, root growth to 0.5-
1cm length is optimal, rejects the bad plant of growth, and 10-15 strains are retained in each culture dish.
(5) the increment measured value of recipient plant B
In plant water extract prepared by above-mentioned steps (2), plus sterilized water dilution is configured to the ratio of volume basis hundred and is
20%th, the leaching liquor of 10%, 5%, 2.5% concentration, takes leaching liquor and processes recipient plant B in above-mentioned steps 4 with micropipettor
Tip of a root part, per plant 5-10ul, pending position shows that then culture dish covers put back to culturing room culture 2-3 again no liquid
My god, measure the main root length of recipient plant B, and the parameter evaluated as growth effect using the increment measured value of recipient plant B；
(6) control treatment
Plant water extract is replaced to process using sterilized water at the tip of a root of above-mentioned steps (4) recipient plant B, processing method,
Condition of culture and time same above-mentioned steps (5) are described；Recipient plant B measurement main root lengths are taken out from culture dish, and to compare
The parameter evaluated as growth effect of increment measured value；
(7) calculating of plant leaching liquor immersion allelopathic effects
The computing formula of plant leaching liquor immersion allelopathic effects is IR=(1-Tr/ck) × 100, and wherein Tr represents recipient plant B
Increment measured value, ck represents the increment measured value of check plant, and IR represents impacts of the donor plant A to recipient plant B
Effect, as IR ＜ 0, represents that donor plant A act as stimulating growth to recipient plant B；As IR ＞ 0, represent that donor is planted
Thing A is inhibited to recipient plant B；As IR=0, represent donor plant A to recipient plant B without activity or expression two
Plant and exist without allelopathy between plant.
Compared with prior art, the invention has the beneficial effects as follows：
1st, using 6% aqueous agar solution, can be disposed vertically as a kind of close solid of medium its state, be conducive to
Recipient plant is in its superficial growth, while providing the moisture needed for plant growth and conveniently processing operation；
2nd, aseptically, plant water extract is also adopted by 0.2 μm of sterilising filtration film and carries out filtration sterilization all operations,
The decomposition of the pollution and microorganism of microorganism to allelochemical is controlled to greatest extent；
3rd, according to the characteristic of plant seedling growth Root Absorption, using donor plant leaching liquor is micro recipient plant root is processed
Point, it is few to the requirement of donor plant, and fully effect of the reaction donor plant extract to acceptor, while eliminating plant
Water extract method needs to carry out the process of osmotic pressure regulation in determining.
4th, eliminating plant leaching liquor immersion needs larger amount of donor plant tissue, direct reaction plant water-soluble substances pair
The influence of other biological, with it is micro accurate the characteristics of, be very suitable for a large amount of screenings of the plant germplasm resource in laboratory
Appraisal.
5th, be it is a kind of effectively, it is economical, quick, accurately, the laboratory plants leaching thing allelopathic effects that can in high volume operate
Evaluation method.
Specific embodiment
With reference to embodiments the present invention is further illustrated.
Embodiment 1：
Test material is paddy rice and barnyard grass, and wherein paddy rice is that donor plant is respectively adopted following 5 kinds：That what is reported is non-
Allelopathic kind Lemont, allelopathic kind PI312777, Koukesumochi, Dongjingbyeo and the K21 for reporting；Acceptor is planted
Thing is barnyard grass.Test is repeated 3 times.
The present embodiment step is as follows：
1st, prepared by culture medium, with the agar medium that concentration of the water preparation in terms of mass fraction is 6%, 120 DEG C, 0.1MPa
Pour into respectively after sterilizing 20min stand-by in circular and square culture dish；
2nd, donor plant is the above-mentioned 5 kinds of paddy rice for referring to, culture dish is placed on after rice paddy seed is fully absorbed water and is placed in 28
DEG C incubator in vernalization 48 hours, in being then seeded in the little basin for being fitted into soil, be placed in 28 DEG C, 12 hours light dark conditions
Chamber planting 20 days, cut paddy rice aerial part, surface is carried out to paddy rice aerial part for 3% sodium hypochlorite with mass fraction and is disappeared
Malicious 5min, then with sterile water wash 3 times, with the blotting paper of sterilizing excessive moisture is suctioned out, and with scissors 1cm or 2cm length is cut into
Segment, in being put into sterilized 10ml test tubes, 1g rice materials add 5ml sterile purified waters, sealing, after 4 DEG C extract 24 hours
Draw leaching liquor with syringe, adopt the content that obtains after the sterilizing of 0.2 μm of sterilising filtration membrane filtration for 20% plant flooding
Liquid, 4 DEG C of Refrigerator stores are standby；
3rd, recipient plant is barnyard grass, is 6% sodium hypochlorite surface sterilization by barnyard grass seed mass fraction, and aqua sterilisa is cleaned
Afterwards uniform sowing is in the above-mentioned 1 circular culture dish for preparing, and aseptic condition is sprouted to showing money or valuables one carries unintentionally；
4th, rule with ruler in the middle part of the square culture dish back side of step 1, sprout in step 3 to the barnyard grass sowing for showing money or valuables one carries unintentionally and exist
In culture dish, rule proper alignment by culture dish back, root downwards, per 20, ware, is placed in the culture dish after capping
28 DEG C of culture is indoor, and light dark is vertically cultivated 3 days within 12 hours, rejects the bad plant of growth, retains the long 0.5-1cm length of main root
Plant, each culture dish stays 15 plants of barnyard grasses；
5th, the plant water extract for preparing step 2, plus sterilized water dilution and be configured to percent by volume for 20%,
10%th, the leaching liquor of 5% 3 concentration gradient, with micropipettor the recipient plant barnyard grass that leaching liquor distinguishes process step 4 is taken
Tip of a root part, per plant 10ul, culture dish is covered again after pending position is dry is put back to culturing room and is cultivated 3 days, measure barnyard grass
The main root length of grass, using the parameter that the main root length measured value of barnyard grass is evaluated as growth effect；
6th, the calculating of plant leaching liquor immersion allelopathic effects, the computing formula of plant leaching liquor immersion allelopathic effects is IR=(1-Tr/
Ck) × 100, wherein Tr represents the increment measured value for the treatment of group recipient plant barnyard grass, and ck represents the increment of control group barnyard grass
Measured value, IR represents influence of the donor plant paddy rice to recipient plant barnyard grass, as IR ＜ 0, represents donor plant paddy rice
Stimulating growth is act as to recipient plant barnyard grass；As IR ＞ 0, represent that donor plant paddy rice has suppression to recipient plant barnyard grass
Make and use；As IR=0, donor plant paddy rice is represented the no activity of recipient plant barnyard grass or represented between two kinds of plants without allelopathic
Effect is present.
Allelopathic effects evaluation result of the paddy rice leaching liquor to barnyard grass in the inventive method of table 1
Embodiment 2：
Test material is paddy rice and lettuce, and wherein paddy rice is that donor plant is respectively adopted following 3 kinds：That what is reported is non-
Allelopathic kind Lemont, allelopathic kind PI312777 reported and Koukesumochi；Recipient plant is lettuce.Test repeats
3 times.
The present embodiment step is as follows：
1st, prepared by culture medium, and the concentration prepared in terms of mass fraction with water is 6% agar medium, and ordinary high pressure sterilizes
Pour into respectively afterwards stand-by in circular and square culture dish；
2nd, donor plant is the above-mentioned 3 kinds of paddy rice for referring to, culture dish is placed on after rice paddy seed is fully absorbed water and is placed in 25
DEG C incubator in vernalization 48 hours, in being then seeded in the little basin for being fitted into soil, be placed in 25 DEG C, 12 hours light dark conditions
Chamber planting 30 days, cut paddy rice aerial part, with mass fraction different cultivars paddy rice aerial part is entered for 6% sodium hypochlorite
Row surface sterilization 5min, then with sterile water wash 3 times, with the blotting paper of sterilizing excessive moisture is suctioned out, with scissors be cut into 1cm or
The segment of 2cm length, in being put into sterilized 10ml test tubes, 1g rice materials add 5ml sterile purified waters, sealing, 4 DEG C of extractions 24
Draw leaching liquor with syringe after hour, adopt the content that obtains after the sterilizing of 0.2 μm of sterilising filtration membrane filtration for 20% plant
Water extract, 4 DEG C of Refrigerator stores are standby；
3rd, recipient plant is lettuce, and lactuca sativa seeds are carried out into surface sterilization, aqua sterilisa for 3% sodium hypochlorite with mass fraction
Clean to tasteless rear uniform sowing to be sprouted under aseptic condition in the above-mentioned 1 circular culture dish for preparing and sprout；
4th, rule with ruler in the middle part of the square culture dish back side of step 1, sprouted lettuce in step 3 and sowed in culture dish
In, to rule proper alignment by culture dish back, the culture dish downwards, per 15, ware, 25 DEG C is placed on after capping by root
Culture is indoor, and light dark is vertically cultivated 2 days within 12 hours, rejects the bad plant of growth, and the long 0.5- of root is retained in each culture dish
10 plants of the lettuce of 1.0cm；
5th, the plant water extract for preparing step 2, plus sterilized water dilution and be configured to percent by volume for 10%, 5%,
The leaching liquor of 2.5% 3 concentration gradient, with micropipettor the recipient plant lettuce tip of a root that leaching liquor distinguishes process step 4 is taken
Part, per plant 5ul, culture dish covers put back to culturing room's culture 2 days again after pending position is dry, measures the main root of lettuce
Length, using the parameter that the main root length measured value of lettuce is evaluated as growth effect；
6th, the calculating of plant leaching liquor immersion allelopathic effects, the computing formula of plant leaching liquor immersion allelopathic effects is IR=(1-Tr/
Ck) × 100, wherein Tr represents the increment measured value for the treatment of group lettuce, and ck represents the increment measured value of control group lettuce, IR
Influence of the donor plant paddy rice to lettuce is represented, as IR ＜ 0, work of the donor plant paddy rice to recipient plant lettuce is represented
With for stimulating growth；As IR ＞ 0, represent that donor plant paddy rice is inhibited to recipient plant lettuce；As IR=0,
Donor plant paddy rice is represented the no activity of recipient plant lettuce or is represented between two kinds of plants without allelopathy presence.
Allelopathic effects evaluation result of the paddy rice leaching liquor to lettuce in the inventive method of table 2
Inventor by paddy rice leaching liquor in the inventive method to recipient plant barnyard grass, the inhibitory action result of lettuce with it is existing
The allelopathic effects evaluation result (being shown in Table 3) of plant water extract method (with embodiment 1, acceptor material is lettuce to donor plant) is compared
Compared with drawing following result：
The paddy rice water extract method of table 3 is to lettuce allelopathic effects evaluation result
Plant water extract method has higher uniformity with the measurement result of the inventive method.Plant water extract method is carried
Liquid is taken using common filtration, the water extract of variable concentrations carry out being needed before acceptor material culture adjusting osmotic potential be it is consistent, with
Exclude to recipient plant growth impact, in addition this method in incubation also easily by microorganism pollution and disturb, shadow
Ring measurement result.
The present invention, as culture matrix, is sprouted using 6% agar for donor plant seed, growth provides enough water
Point, contribute to the growth of plant；Culture dish is disposed vertically can make plant growth on the surface of culture medium, convenient to process operation.
All operations of the present invention are aseptically carried out, and the interference and microorganism that microorganism is controlled to greatest extent may be to changing
The decomposition of sense material.Further, since directly processing recipient plant growing point (tip of a root), the amount pole of required water extract
It is few, not only simplify operation and improve efficiency but also cost-effective.
Claims (1)
1. a kind of micro plant hydrotrope allelopathic effects evaluate bioassay method, it is characterised in that the micro plant water after improvement
Molten materialization sense Potential Evaluation bioassay method, comprises the following steps that:
(1) prepared by culture medium
It is >=6% agar solution to prepare concentration in terms of mass fraction with water, by the agar solution for preparing in 120 DEG C, 0.1MPa
Sterilizing 20min pours into stand-by in circular and square culture dish；
(2) culture of donor plant A and water extract prepare
Described donor plant A seeds are placed on culture dish after fully absorbing water to be placed in vernalization 48 in 25 DEG C or 28 DEG C of culturing room little
When, in being then seeded in the little basin for being fitted into soil, it is placed in 25 DEG C or 28 DEG C, the chamber planting 20-30 of 12 hours light dark conditions
My god, clip aerial part is that 3-6% sodium hypochlorite carries out surface sterilization 5min with mass fraction, then uses sterile water wash 2-3
It is secondary to tasteless, excessive moisture is suctioned out with the blotting paper of sterilizing, donor plant A scissors is cut into the segment of 1-2cm length, it is put into nothing
In bacterium container, by donor plant A and mass volume ratio=1 of aqua sterilisa:5-10 adds sterilizing manhole cover, 4 DEG C of shaking tables to extract 24
Hour, leaching liquor is drawn with syringe, the aseptic plant flooding of 20%-10% is obtained using 0.2 μm of sterilising filtration membrane filtration
Liquid, 4 DEG C of Refrigerator stores are standby；
(3) the surface of the seed sterilization and sprouting of recipient plant B
It with mass fraction is that 3-6% sodium hypochlorite is planted to recipient plant B that the surface of the seed sterilization of described recipient plant B is
Sub- surface sterilization 10min, then with sterile water wash 2-3 time to tasteless, is then seeded in recipient plant B above-mentioned 1 and prepares
In the culture medium of circular culture dish, aseptic condition sprout standby to showing money or valuables one carries unintentionally；
(4) sowing of recipient plant B and culture
The middle part at the square culture dish back side in above-mentioned steps 1 is rule with ruler, is planted sprouting in step 3 to the acceptor for showing money or valuables one carries unintentionally
Thing B is sowed into culture dish, is rule by culture dish back and neatly sow, and per ware 15-20 grains, is placed in the culture dish after capping
Indoor, the light dark culture in 12 hours 3-5 days in 25 DEG C or 28 DEG C of cultures, root is cultivated downward vertically, root growth to 0.5-1cm
Length is optimal, rejects the bad plant of growth, and 10-15 strains are retained in each culture dish；
(5) the increment measured value of recipient plant B
In plant water extract prepared by above-mentioned steps (2), plus sterilized water dilution be configured to volume basis hundred than for 20%,
10%th, the leaching liquor of 5%, 2.5% concentration, with micropipettor the tip of a root that leaching liquor processes recipient plant B in above-mentioned steps 4 is taken
Part, per plant 5-10ul, pending position shows that then culture dish covers again and puts back to culturing room and cultivate 2-3 days no liquid, surveys
The main root length of amount recipient plant B, and the parameter evaluated as growth effect using the increment measured value of recipient plant B；
(6) control treatment
Plant water extract is replaced to process using sterilized water at the tip of a root of above-mentioned steps (4) recipient plant B, processing method, culture
Condition and time same above-mentioned steps (5) are described；Recipient plant B measurement main root lengths, and the life to compare are taken out from culture dish
It is long to measure the parameter that definite value is evaluated as growth effect；
(7) calculating of plant leaching liquor immersion allelopathic effects
The computing formula of plant leaching liquor immersion allelopathic effects is IR=(1-Tr/ck) × 100, and wherein Tr represents the life of recipient plant B
Long to measure definite value, ck represents the increment measured value of check plant, and IR represents influences of the donor plant A to recipient plant B,
As IR ＜ 0, represent that donor plant A act as stimulating growth to recipient plant B；As IR ＞ 0, donor plant A pair is represented
Recipient plant B is inhibited；As IR=0, represent donor plant A to recipient plant B without the two kinds of plants of activity or expression
Exist without allelopathy between thing.
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| 郭怡卿等: "46 种作物水浸提液对莴苣的化感潜力", 《西南农业学报》 * |
| 陶俊杰等: "青藏高原干旱地区8种主栽小麦品种对旱雀麦的化感作用评价", 《干旱地区农业研究》 * |
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