CN107836348A - A kind of implantation methods of pearl Lee - Google Patents

A kind of implantation methods of pearl Lee Download PDF

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Publication number
CN107836348A
CN107836348A CN201711045171.6A CN201711045171A CN107836348A CN 107836348 A CN107836348 A CN 107836348A CN 201711045171 A CN201711045171 A CN 201711045171A CN 107836348 A CN107836348 A CN 107836348A
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seedling
extract
explant
pearl lee
culture
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王明松
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Tian'e County Eight La Yao Township Yimin Vegetable Cooperatives
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Tian'e County Eight La Yao Township Yimin Vegetable Cooperatives
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Priority to CN201711045171.6A priority Critical patent/CN107836348A/en
Publication of CN107836348A publication Critical patent/CN107836348A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00—Calcareous fertilisers
    • C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A01G2013/004—Liquid mulch

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Abstract

The present invention relates to technical field of plant culture, discloses a kind of implantation methods of pearl Lee, and invention selects the method for tissue cultures to shorten seedling raise period to pearl lijin's row nursery；During nursery, the disinfectant formed using plant extracts configuration substitutes conventional chemosterilant, simultaneously, during explant culture and rooted seedling culture is carried out, culture medium I, medium ii, planted medium and foliar fertilizer are configured according to the growth characteristics of explant, rooted seedling, seedling, explant, the germination percentage of rooted seedling, survival rate can be effectively improved, seedling quality can be effectively improved；Shorten seedling raise period, invention configures organic fertilizer, organic liquid manure, expelling parasite water always according to the characteristic of tissue cultures seedling and pearl Lee seedling is planted, and while promoting pearl Lee's fruit tree growth, will not also bring environmental pollution.

Description

A kind of implantation methods of pearl Lee
【Technical field】
The present invention relates to technical field of plant culture, more particularly to a kind of implantation methods of pearl Lee.
【Background technology】
Pearl Lee is the peculiar kind in Guangxi, and pearl Lee has in seedling raising process to be obtained usually through grafting, artificial pollination breeding ；The cultivation cycle of grafting culture pearl Lee is usually 1-2, and the cultivation cycle of grafting culture is grown, and cultural method is complicated, And the artificial pollination cycle is longer, means and its complexity, workload is very big, and tissue cultures can effectively shorten seedling raise period, at present The report on pearl Lee's tissue cultures is yet there are no, because pearl Lee's kind is more rare, is not planted extensively also； And the easy microbiological contamination of explant of pearl Lee fruit tree, browning is caused, it is sensitive to bactericidal agent, therefore culture culture according to conventional methods Pearl Lee is not easy to survive, and at present, in the tissue culture procedures for carrying out plant, the conventional antibacterial disinfectant in China is mainly with liter Based on mercury, substantial amounts of research is carried out to the toxic action of mercury both at home and abroad, it was demonstrated that mercury has weight to each growth course of cell Influence, so the explant to be sterilized by mercuric chloride, need to thoroughly remove the Hg+ of residual, it is external with the residual mercuric chloride for eliminating micro The injury of implant, although domestic and international researcher has carried out widely studied and screening to disinfectant for many years, does not find so far A kind of disinfectant that can meet ideal conditions.What conventional tissue culture medium (TCM) was selected is MS culture mediums, and culture medium raw material is more expensive, matches somebody with somebody Put complexity, and during as big production, cost is larger, therefore, in the mistake using tissue cultures to the progress nursery of pearl Lee fruit tree , it is necessary to find out suitable pearl Lee's rearing condition in journey, germination percentage, the survival rate of pearl Lee fruit tree can not only be improved, moreover it is possible to contract The seedling raise period of short pearl Lee fruit tree, production cost is reduced, will not also cause environmental pollution.
Pearl Lee seedling is after tissue cultures, and its growing environment is in perfect condition, if without appropriate ways Hardening, cultivation will largely effect on the quality of pearl Lee seedling, and therefore, plantation pearl Lee seedling can not be planted with conventional method Plant, it is necessary to find out a kind of implantation methods for pearl Lee's growth of seedling that suitable tissue cultures come out.
【The content of the invention】
In view of the above, it is necessary to a kind of implantation methods of pearl Lee are provided, the product of pearl Lee seedling can be effectively improved Matter, and a kind of implantation methods for pearl Lee's growth of seedling that suitable tissue cultures come out can also be provided, pearl Lee fruit can be promoted While tree growth, environmental pollution will not be also brought.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of implantation methods of pearl Lee, this method includes the culture of pearl Lee seedling and pearl Lee plants two steps：
(1) pearl Lee seedling is cultivated：
(1) culturing room sterilizes：Before culture 6h-8h according to 400g/m2-600g/m2 fountain height to the culture closed the doors and windows Thimerosal carries out sterilization in spray booth in room；
(2) explant pre-processes：Select the terminal bud of pearl Lee fruit tree and subtract unnecessary blade as explant, explant length For 2cm-3cm, a diameter of 0.4cm-0.6cm；Explant is rinsed with water, drain after be put into explant thimerosal and carry out Immersion, a length of 50min-60min obtains the explant pre-processed during immersion；
(3) explant culture：Step (2) pretreated explant is put into culture medium I and cultivated, cultivation temperature For 24 DEG C -26 DEG C, intensity of illumination 1000Lx-1500Lx, light application time 12h-16h, until explant grows sprouting；It is described Explant and culture medium I mass ratio are 1:25-30；The illumination carries out circulation light photograph, monochromatic circulation using monochromatic light Illumination order is " the white lamp 20min- green lights 20min- blue lamps 20min- red lights 20min " of red light 20min-；
(4) squamous subculture：The explant that sprouting is grown in step (3) is transferred and carries out squamous subculture in medium ii, Carry out squamous subculture in squamous subculture case, cultivation temperature is 25 DEG C -28 DEG C, intensity of illumination 1300Lx-1700Lx, during illumination Between be 20h-24h, and subculture medium is constantly changed, until explant grows the high rooted seedlings of 5cm-7cm；The illumination uses Monochromatic light carries out circulation light photograph, and monochromatic circulation light is " tangerine lamp 30min- blue lamp 30min- red light 30min- green lights according to order 30min- tangerine lamps 30min "；
(5) seedling is cultivated：The rooted seedling that step (4) obtains is put into the nutrition cup equipped with planted medium to be put into greenhouse Cultivated, the greenhouse temperature is 22 DEG C -28 DEG C, and illumination is solar radiation；Every 15d-20d to taking root during rooted seedling culture Seedling sprays foliar fertilizer according to 70g/ strain -90g/ strains；Under growth root height of seedling degree grows to 15cm-20cm and obtains pearl Lee seedling；
(2) pearl Lee plants：
A, readjustment of field plot：Pre- kind is ploughed, afterwards according to 3kg/m2-4kg/m2Applied amount thrown into pre- kind ground Spill and kill pulverized limestone and carry out disinfection, and the plot after offseting poison with mulch film is covered, and is opened mulch film after covering 30d-40d, is gone forward side by side Row is secondary to plough, then according to 2kg/m2-3kg/m2Dose to after secondary plough soil apply organic fertilizer, enter again afterwards Row is ploughed three times, and covers mulch film, is made compost 20d-30d and is obtained planting plot；
B, pearl Lee is planted：The cultured pearl Lee seedling of step (5) is colonized according to 2m-3m × 2m-3m seeding row spacing In step A plantation plot, according to 800g/m after field planting2-1200g/m2Dose apply organic liquid manure, afterwards at interval of 15d-20d applies a liquid manure into plantation plot, applies 9 times -12 times altogether；Planting process is irregularly applied to pearl Lee seedling Add expelling parasite water, and it is 30%-40% to keep the relative humidity of soil.
Further, the indoor thimerosal of the step (1) is made up of the composition of following percentage by weight：5%-7%'s Pepper extract, 5%-7% Herba Fimbristylis dichotomae extract, 1%-3% sodium hypochlorite, 1%-3% sodium chloride, 1%-3% hydrogen Calcium oxide, surplus are the ethanol solution that percentage by volume is 95%.
Further, the explant thimerosal of the step (1) is made up of the composition of following percentage by weight：5%-7% Shrubby sophora extract, 5%-7% capsicum extract, 5%-7% garlic P.E, 2%-5% chitosan, 5%-7% Wrinkled giant hyssop extract solution, surplus be ethanol solution that percentage by volume is 75%.
Further, the culture medium I of the step (3) is made up of the composition of following percentage by weight：20%-30% silkworm Husky soak, 5%-7% agar, 2%-5% trehalose, 2%-5% spirulina extract, 3%-6% aloe carry It is water to take thing, 1%-3% magnesium sulfate, 1%-3% sodium chloride, surplus.
Further, the medium ii of the step (4) is made up of the composition of following percentage by weight：40%-50%'s Silkworm excrement soak, 5%-7% agar, 2%-5% trehalose, 2%-5% Chinese toon leaf extract, 3%-6% Chinese prickly ash carry It is water to take thing, 1%-3% magnesium sulfate, 1%-3% sodium dihydrogen phosphate, surplus.
Further, step (5) planted medium is made up of the composition of following percentage by weight：30%-35%'s The peanut shell of beans shell, 20%-30%, 20%-30% stalk, 5%-15% loess, 5%-10% organic fertilizer.
Further, the foliar fertilizer of the step (5) is made up of the composition of following percentage by weight：30%-40% urine Liquid dilution, 5%-7% pepper extract, 3%-5% papaya extractives, 3%-5% humic acid.
Further, the step A organic fertilizers comprise the following components in parts by weight：13 parts -17 parts of lime stone, 23 Parts -34 parts of silkworm excrement, 12 parts -17 parts of stalk, 17 parts -27 parts of tea seed shell, 12 parts -24 parts of Fructus Chaenomelis ferment and 14 parts - 22 parts of waste and old bacterium bag.
Further, the composition composition of the following percentage by weight of the organic liquid manure of step B：10%-15% mulberry leaf ferment Element, 5%-7% potassium dihydrogen phosphate, 5%-7% nitric acid ammonia, 2%-7% silkworm slough off extract, surplus is that the content of organic matter is 5%-8% silkworm excrement soak.
Further, the composition composition of the following percentage by weight of the step B expelling parasite water：1%-3% betelnut extract, 1%-3% mint extract, 1%-3% macrostachys uraria herb extract, 1%-3% Andrographis Paniculata, surplus are solute matter Measure the sodium chloride solution that percentage is 3%-5%.
The present invention has the advantages that：
1st, the present invention can shorten seedling raise period from the method for tissue cultures to pearl lijin's row nursery；During nursery, use The disinfectant formed by plant extracts configuration substitutes conventional chemosterilant, the composition and percentage by weight of the disinfectant For：5%-7% shrubby sophora extract, 5%-7% capsicum extract, 5%-7% garlic P.E, 2%-5% shell gather Sugar, 5%-7% wrinkled giant hyssop extract solution, surplus are the ethanol solution that percentage by volume is 75%；Active ingredient in extract is equal The effect of antibacterial sterilization can be effectively played；Meanwhile during explant culture and rooted seedling culture is carried out, according to explant Body, rooted seedling, the growth characteristics of seedling configuration culture medium I, medium ii, planted medium and foliar fertilizer, can be effectively improved outer Implant, the germination percentage of rooted seedling, survival rate, can effectively improve seedling quality；Shorten seedling raise period.
2nd, pearl Lee seedling of tissue cultures is less susceptible to Adaptable growth environment, therefore, Shen than the seedling of conventional cultivating seeds Asking someone need to be according to the supporting corresponding planting measure of pearl Lee's seedling characteristic of cultivation, therefore, and applicant is configured with organic fertilizer, organic Liquid manure, expelling parasite water are planted to pearl Lee seedling, because the related reagent that the application voluntarily configures is plant extracts, Seedling culture and planting process are all without causing environmental pollution caused by chemical reagent.
【Embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive Feature and/or step beyond, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated otherwise, Replaced by other equivalent or with similar purpose alternative features.I.e., unless specifically stated otherwise, each feature is a series of An example in equivalent or similar characteristics.
Embodiment 1：
A kind of implantation methods of pearl Lee, this method includes the culture of pearl Lee seedling and pearl Lee plants two steps：
(1) pearl Lee seedling is cultivated：
(1) culturing room sterilizes：6h is according to 400g/m before culture2Fountain height into the culturing room closed the doors and windows in spray booth Thimerosal carries out sterilization；
(2) explant pre-processes：Select the terminal bud of pearl Lee fruit tree and subtract unnecessary blade as explant, explant length For 2cm, a diameter of 0.4cm；Explant is rinsed with water, drain after be put into explant thimerosal and soaked, soak Shi Changwei 50min obtain the explant pre-processed；
(3) explant culture：Step (2) pretreated explant is put into culture medium I and cultivated, cultivation temperature For 24 DEG C, intensity of illumination 1000Lx, light application time 12h, until explant grows sprouting；The explant and culture medium I Mass ratio be 1:25-30；The illumination carries out circulation light photograph using monochromatic light, and monochromatic circulation light is " red light according to order The white lamp 20min- green lights 20min- blue lamps 20min- red lights 20min " of 20min-；
(4) squamous subculture：The explant that sprouting is grown in step (3) is transferred and carries out squamous subculture in medium ii, Squamous subculture is carried out in squamous subculture case, cultivation temperature is 25 DEG C, intensity of illumination 1300Lx, light application time 20h, not It is disconnected to change subculture medium, until explant grows the high rooted seedlings of 5cm；The illumination carries out circulation light photograph using monochromatic light, Monochromatic circulation light is " tangerine lamp 30min- blue lamp 30min- red light 30min- green light 30min- tangerine lamps 30min " according to order；
(5) seedling is cultivated：The rooted seedling that step (4) obtains is put into the nutrition cup equipped with seedling culture medium to be put into greenhouse Cultivated, the greenhouse temperature is 22 DEG C, and illumination is solar radiation；During rooted seedling culture every 15d to rooted seedling according to Foliar fertilizer is sprayed in 70g/ strains；Under growth root height of seedling degree grows to 15cm and obtains pearl Lee seedling；
(2) pearl Lee plants：
A, readjustment of field plot：Pre- kind is ploughed, afterwards according to 3kg/m2Applied amount shed into pre- kind ground and kill stone Ashes is carried out disinfection, and the plot after offseting poison with mulch film is covered, and mulch film is opened after covering 30d, and carry out it is secondary plough, Then according to 2kg/m2Dose to after secondary plough soil apply organic fertilizer, ploughed three times again afterwards, and cover Mulch film, make compost 20d and obtain planting plot；
B, pearl Lee is planted：The cultured pearl Lee seedling of step (5) is colonized in step A according to 2m × 2m seeding row spacing Plantation plot in, according to 800g/m after field planting2Dose apply organic liquid manure, afterwards at interval of 15d into plantation plot Apply a liquid manure, apply 9 times altogether；Planting process irregularly applies expelling parasite water to pearl Lee seedling, and keeps the phase of soil It is 30% to humidity.
Wherein, the indoor thimerosal of step (1) is made up of the composition of following percentage by weight：5% pepper extract, 5% Herba Fimbristylis dichotomae extract, 1% sodium hypochlorite, 1% sodium chloride, 1% calcium hydroxide, surplus are that percentage by volume is 95% ethanol solution.
1. the extracting method of pepper extract is in above-mentioned indoor thimerosal：The Chinese prickly ash that moisture content is 5% is crushed, Screened to obtain Pricklyash peel by the screen cloth of 40 mesh sieves, according to mass ratio be 1 by zanthoxylum powder and petroleum ether:4 are mixed, It is then placed in refluxing extraction device, refluxing extraction is carried out under the conditions of 100 DEG C, after extracting 12h, filters off except filter residue, take filtrate Rotary evaporation concentration is carried out until moisture content obtains pepper extract for 4%；The content of amide substance is in extract 102.87mg/g, the content of lignanoid are 97.09mg/g, tonka-bean cellulose content is 113.98mg/g, the content of terpene substances is 84.93mg/g。
2. the extracting method of Herba Fimbristylis dichotomae extract is in above-mentioned indoor thimerosal：Fresh fiddlehead is smashed to pieces and is with percentage by volume 75% ethanol solution is 1 according to mass ratio:3 are mixed, and refluxing extraction is then carried out under the conditions of 95 DEG C, after extracting 23h, Filter off and remove filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains pepper extract for 4%；Flavones in extract The content of class material is 302.31mg/g.
3. the collocation method of above-mentioned indoor thimerosal is：Pepper extract, fiddlehead extraction are weighed by above-mentioned percentage by weight Thing, sodium hypochlorite, sodium chloride and calcium hydroxide are uniformly mixed, and then pour into 95% ethanol solution in mixture, will Percent of total configuration is made to 100%.
Wherein, the explant thimerosal of step (1) is made up of the composition of following percentage by weight：5% shrubby sophora extract, 5% capsicum extract, 5% garlic P.E, 2% chitosan, 5% agastache extract surplus are that percentage by volume is 75% ethanol solution.
1. the extracting method of shrubby sophora extract is in above-mentioned explant thimerosal：The kuh-seng that moisture content is 5% is subjected to powder It is broken, screened to obtain Lightyellow Sophora Root by the screen cloth of 40 mesh sieves, by the butanol solution that kuh-seng powder and percentage by volume are 20% It is 1 according to mass ratio:3 are mixed, and are then placed in refluxing extraction device, and refluxing extraction is carried out under the conditions of 90 DEG C, extract 18h Afterwards, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains shrubby sophora extract for 4%；In extract The content of matrine is 542.98mg/g, saponin content 23.76mg/g.
2. the extracting method of pepper extract is in above-mentioned explant thimerosal：The capsicum that moisture content is 5% is subjected to powder It is broken, screened to obtain chilli powder by the screen cloth of 40 mesh sieves, by the ethanol solution that chilli powder and percentage by volume are 70% It is 1 according to mass ratio:5 are mixed, and are then placed in refluxing extraction device, and refluxing extraction is carried out under the conditions of 90 DEG C, extract 20h Afterwards, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains pepper extract for 4%；In extract Ascorbic content is 216.65mg/g, capsaicin content 184.87mg/g.
3. the extracting method of garlic P.E is in above-mentioned explant thimerosal：Fresh garlic is smashed to pieces and percentage by volume According to mass ratio it is 1 for 55% acetum:4 are mixed, and are then placed in refluxing extraction device, are carried out under the conditions of 95 DEG C Refluxing extraction, after extracting 24h, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains kuh-seng for 4% Extract；The content of allicin is 423.09mg/g in extract.
4. the extracting method of agastache extract is in above-mentioned explant thimerosal：Fresh wrinkled giant hyssop branches and leaves are smashed to pieces and volume hundred The ethanol solution that fraction is 70% is 1 according to mass ratio:3 are mixed, and are then placed in refluxing extraction device, under the conditions of 98 DEG C Refluxing extraction is carried out, after extracting 20h, is filtered off except filter residue, takes filtrate to carry out rotary evaporation concentration until moisture content obtains for 4% Agastache extract；The content of wrinkled giant hyssop alcohol is 387.09mg/g in extract, and alkenes content of material is 19.07mg/g.
5. the collocation method of above-mentioned explant thimerosal is：Shrubby sophora extract is weighed by above-mentioned percentage by weight, capsicum carries Take liquid, garlic P.E, chitosan, agastache extract uniformly to be mixed, 75% ethanol solution is then poured into mixture In, by percent of total configuration system to 100%.
Wherein, the culture medium I of step (3) is made up of the composition of following percentage by weight：20% silkworm excrement soak, 5% Agar, 2% trehalose, 2% spirulina extract, 3% aloe extract, 1% magnesium sulfate, 1% sodium chloride, Surplus is water.
1. the immersion process of silkworm excrement soak is in above-mentioned culture medium I：According to mass ratio it is 2 by silkworm excrement and distilled water:1 enters Row mixing, then heats the mixture to 80 DEG C and filters to take filtrate and concentrated, be concentrated to that concentrate is stoste volume 1/3 To silkworm excrement soak.
2. the extracting method of spirulina extract is in above-mentioned culture medium I：Fresh spirulina is smashed to pieces and percentage by volume According to mass ratio it is 1 for 50% ethyl ester solution:4 are mixed, and are then placed in refluxing extraction device, are entered under the conditions of 100 DEG C Row refluxing extraction, after extracting 18h, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains spiral shell for 4% Revolve algae extract；Ascorbic content is 123.81mg/g, total amino acid content 342.98mg/g in extract.
3. the extracting method of aloe extract is in above-mentioned culture medium I：It is with percentage by volume after aloe peeling is smashed to pieces 50%% butanol solution is 1 according to mass ratio:4 are mixed, and are then placed in refluxing extraction device, are carried out under the conditions of 92 DEG C Refluxing extraction, after extracting 21h, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains aloe for 4% Extract；The content of barbaloin is 216.09mg/g in extract, Vitamin C content 87.09mg/g, and aloin content is 109.07mg/g。
4. above-mentioned culture medium I collocation method is：By above-mentioned percentage by weight weigh silkworm excrement soak, agar, trehalose, Spirulina extract, aloe extract, magnesium sulfate, sodium chloride are uniformly mixed, and then water is poured slowly into mixture will Percent of total configuration system is heated to 70 DEG C and carries out constant temperature stirring 5min to 100%, is cooled to room temperature and obtains the culture medium I.
Wherein, the medium ii of step (4) is made up of the composition of following percentage by weight：40% silkworm excrement soak, 5% Agar, 2% trehalose, 2% Chinese toon leaf extract, 3% pepper extract, 1% magnesium sulfate, 1% biphosphate Sodium, surplus are water.
1. the immersion process of silkworm excrement soak is in above-mentioned medium ii：According to mass ratio it is 2 by silkworm excrement and distilled water:1 Mixed, then heat the mixture to 80 DEG C and filter to take filtrate and concentrated, be concentrated to that concentrate is stoste volume 1/3 Obtain silkworm excrement soak.
2. the extracting method of Chinese toon leaf extract is in above-mentioned medium ii：It is with percentage by volume after fresh Chinese toon leaf is smashed to pieces 95% ethanol solution is 1 according to mass ratio:4 are mixed, and are then placed in refluxing extraction device, are returned under the conditions of 95 DEG C Extraction is flowed, after extracting 19h, is filtered off except filter residue, takes filtrate to carry out rotary evaporation concentration and is carried until moisture content obtains wrinkled giant hyssop for 4% Take thing；The content of toonin is 102.09mg/g, Vitamin C content 87.09mg/g in extract.
3. the extracting method of pepper extract is in above-mentioned medium ii：The Chinese prickly ash that moisture content is 5% is crushed, passed through The screen cloth for crossing 40 mesh sieves is screened to obtain Pricklyash peel, according to mass ratio is 1 by zanthoxylum powder and petroleum ether:4 are mixed, so After be put into refluxing extraction device, carry out refluxing extraction under the conditions of 100 DEG C, after extracting 12h, filter off except filter residue, take filtrate to enter The concentration of row rotary evaporation obtains pepper extract until moisture content for 4%；The content of amide substance is in extract 102.87mg/g, the content of lignanoid are 97.09mg/g, tonka-bean cellulose content is 113.98mg/g, the content of terpene substances is 84.93mg/g。
4. the collocation method of above-mentioned medium ii is：Silkworm excrement soak, agar, marine alga are weighed by above-mentioned percentage by weight Sugar, Chinese toon leaf extract, pepper extract, magnesium sulfate, sodium dihydrogen phosphate are uniformly mixed, and water then is poured slowly into mixture It is middle to make percent of total configuration to 100%, and be heated to 70 DEG C and carry out constant temperature stirring 5min, it is cooled to room temperature and obtains the culture Base II.
Wherein, step (5) seedling culture medium is made up of the composition of following percentage by weight：30% beans shell, 20% flower Raw shell, 30% stalk, 10% loess, 10% organic fertilizer.
1. the preparation method of above-mentioned seedling culture medium is：Weigh beans shell, peanut shell by above-mentioned percentage, stalk, loess and Organic fertilizer, beans shell, peanut shell and stalk are mixed and crushed, then be mixed to get educating with loess and organic fertilizer Seedling culture medium.
Wherein, the foliar fertilizer of step (5) is made up of the composition of following percentage by weight：30% urine dilution, 5% Pepper extract, 3% papaya extractives, 3% humic acid, surplus are water.
1. the collocation method of urine dilution is in above-mentioned foliar fertilizer：According to mass ratio it is 1 by people and animals' urine and water:2 enter Row is mixed to get above-mentioned urine dilution.
2. the extracting method of pepper extract is in above-mentioned foliar fertilizer：The capsicum that moisture content is 5% is crushed, passed through The screen cloth of 40 mesh sieves is screened to obtain chilli powder, by ethanol solution that chilli powder and percentage by volume are 70% according to quality Than for 1:5 are mixed, and are then placed in refluxing extraction device, and refluxing extraction is carried out under the conditions of 90 DEG C, after extracting 20h, filtering Filter residue is removed, takes filtrate to carry out rotary evaporation concentration until moisture content obtains pepper extract for 4%；Vitamin C in extract Content be 216.65mg/g, capsaicin content 184.87mg/g.
3. the extracting method of papaya extractives is in above-mentioned foliar fertilizer：Fresh pawpaw belt leather is smashed to pieces, afterwards and body The ethanol that product percentage is 75% is 1 according to mass ratio:5 are mixed, and are then placed in refluxing extraction device, in 100 DEG C of conditions Lower carry out refluxing extraction, after extracting 26h, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content obtains for 4% To papaya extractives；Total amino acid content 210.92mg/g in extract, protein content 211.98mg/g.
4. the collocation method of above-mentioned foliar fertilizer is：Urine dilution, pepper extract, wood are weighed by above-mentioned percentage by weight Melon extract, humic acid are uniformly mixed, and then water is poured slowly into percent of total configuration system to 100% in mixture, And be heated to 50 DEG C and carry out constant temperature stirring 5min, it is cooled to room temperature and obtains the foliar fertilizer.
Wherein, step A organic fertilizers comprise the following components in parts by weight：13 parts of lime stone, 23 parts of silkworm excrement, 12 parts Stalk, 17 parts of tea seed shell, 12 parts of Fructus Chaenomelis ferment and 14 parts of waste and old bacterium bag.
1. the preparation method of Fructus Chaenomelis ferment is in above-mentioned organic fertilizer：Pawpaw is smashed to pieces, then according to pawpaw and Angel activity Dry ferment ratio is 50:1 mass ratio is mixed, and is 27 DEG C in temperature, and fermentation 23d is made under conditions of humidity is 20%.
2. waste and old bacterium bag is selected from the waste and old bacterium bag after one batch of agaric and phoenix-tail mushroom culture in above-mentioned organic fertilizer.
3. the preparation method of above-mentioned organic fertilizer is：Lime stone, silkworm excrement, stalk, tea seed shell, wood are weighed by above-mentioned parts by weight Melon ferment and waste and old bacterium bag are mixed, and are crushed and obtained the organic fertilizer.
Wherein, the composition composition of the following percentage by weight of the organic liquid manure of step B：10% mulberry leaf ferment, 5% di(2-ethylhexyl)phosphate Hydrogen potassium, 5% nitric acid ammonia, 2% silkworm slough off extract, surplus is silkworm excrement soak that the content of organic matter is 5%.
1. the preparation method of mulberry leaf ferment is in above-mentioned organic liquid manure：New fresh mulberry leaf is smashed to pieces, then according to mulberry leaf and peace Fine jade active dry yeast ratio is 60:1 mass ratio is mixed, and is 28 DEG C in temperature, and humidity is fermented 32d under conditions of being 15% It is made.
2. the extracting method that silkworm sloughs off extract in above-mentioned organic liquid manure is：The silkworm that moisture content is 5% is sloughed off and crushed, is passed through The screen cloth for crossing 100 mesh sieves is screened to obtain silkworm and sloughs off powder, by silkworm slough off powder and ethanol solution that percentage by volume is 95% according to Mass ratio is 1:4 are mixed, and are then placed in refluxing extraction device, and refluxing extraction is carried out under the conditions of 80 DEG C, after extracting 24h, Filter off and remove filter residue, take filtrate to carry out rotary evaporation concentration and slough off extract until moisture content obtains silkworm for 4%；Total ammonia in extract The content of base acid is 320.87mg/g.
3. the immersion process of silkworm excrement soak is in above-mentioned organic liquid manure：According to mass ratio it is 3 by silkworm excrement and water:1 is carried out Mixing, 60 DEG C are then heated the mixture to, constant temperature stirring 40min, filtering, takes filtrate to obtain silkworm excrement soak.
4. the collocation method of above-mentioned organic liquid manure is：Mulberry leaf ferment, potassium dihydrogen phosphate, nitre are weighed by above-mentioned percentage by weight Sour ammonia, silkworm slough off extract and uniformly mixed, and the silkworm excrement soak water that the content of organic matter is 5% then is poured slowly into mixture It is middle by percent of total configuration system to 100%, and be heated to 50 DEG C carry out constant temperature stirring 5min, be cooled to room temperature obtain it is described organic Liquid manure.
Wherein, the composition composition of the following percentage by weight of step B expelling parasites water：1% betelnut extract, 1% peppermint carry It is the sodium chloride that Solute mass percentage is 3% to take thing, 1% macrostachys uraria herb extract, 1% Andrographis Paniculata, surplus Solution.
1. the extracting method of betelnut extract is in above-mentioned expelling parasite water：Fresh areca leaf is smashed to pieces, afterwards and volume basis The ethanol that number is 80% is 1 according to mass ratio:3 are mixed, and are then placed in refluxing extraction device, are returned under the conditions of 90 DEG C Extraction is flowed, after extracting 21h, is filtered off except filter residue, takes filtrate to carry out rotary evaporation concentration and is carried until moisture content obtains betel nut for 4% Take thing；Total amino acid content 108.92mg/g in extract, vitamin content 109.65mg/g.
2. the extracting method of mint extract is in above-mentioned expelling parasite water：The whole strain of fresh mint is smashed to pieces, afterwards and body The ethanol that product percentage is 80% is 1 according to mass ratio:4 are mixed, and are then placed in refluxing extraction device, under the conditions of 90 DEG C Refluxing extraction is carried out, after extracting 23h, is filtered off except filter residue, takes filtrate to carry out rotary evaporation concentration until moisture content obtains for 4% Mint extract；Vitamin content 243.87mg/g in extract, menthones total content are 97.09mg/g.
3. the extracting method of macrostachys uraria herb extract is in above-mentioned expelling parasite water：The whole strain of fresh macrostachys uraria herb is smashed to pieces, afterwards According to mass ratio it is 1 with the ethanol that percentage by volume is 95%:5 are mixed, and are then placed in refluxing extraction device, at 100 DEG C Under the conditions of carry out refluxing extraction, after extracting 20h, filter off except filter residue, take filtrate to carry out rotary evaporation concentration until moisture content is 4% obtains macrostachys uraria herb extract；Vitamin total content 215.54mg/g in extract.
4. the extracting method of Andrographis Paniculata is in above-mentioned expelling parasite water：The whole strain of fresh Herba Andrographitis is smashed to pieces, afterwards According to mass ratio it is 1 with the ethanol that percentage by volume is 80%:4 are mixed, and are then placed in refluxing extraction device, in 95 DEG C of bars Refluxing extraction is carried out under part, after extracting 24h, is filtered off except filter residue, takes filtrate to carry out rotary evaporation concentration until moisture content is 4% Obtain Andrographis Paniculata；Total amino acid content 321.09mg/g in extract, vitamin content 87.93mg/g
5. the collocation method of above-mentioned expelling parasite water is：Betelnut extract, mint extract, cloth are weighed by above-mentioned percentage by weight Dog tail extract, Andrographis Paniculata thing are uniformly mixed, and then delay the sodium chloride solution that matter mass percent is 3% It is slow to pour into percent of total configuration system in mixture to 100%, and be heated to 50 DEG C and carry out constant temperature stirring 5min, it is cooled to room temperature Obtain the expelling parasite water.
Embodiment 2：
A kind of implantation methods of pearl Lee, this method includes the culture of pearl Lee seedling and pearl Lee plants two steps：
(1) pearl Lee seedling is cultivated：
(1) culturing room sterilizes：8h is according to 600g/m before culture2Fountain height into the culturing room closed the doors and windows in spray booth Thimerosal carries out sterilization；
(2) explant pre-processes：Select the terminal bud of pearl Lee fruit tree and subtract unnecessary blade as explant, explant length For 3cm, a diameter of 0.6cm；Explant is rinsed with water, drain after be put into explant thimerosal and soaked, soak Shi Changwei 60min obtain the explant pre-processed；
(3) explant culture：Step (2) pretreated explant is put into culture medium I and cultivated, cultivation temperature For 26 DEG C, intensity of illumination 1500Lx, light application time 12h-16h, until explant grows sprouting；The explant and culture Base I mass ratio is 1:30；The illumination carries out circulation light photograph using monochromatic light, and monochromatic circulation light is " red light according to order The white lamp 20min- green lights 20min- blue lamps 20min- red lights 20min " of 20min-；
(4) squamous subculture：The explant that sprouting is grown in step (3) is transferred and carries out squamous subculture in medium ii, Squamous subculture is carried out in squamous subculture case, cultivation temperature is 28 DEG C, intensity of illumination 1700Lx, light application time 24h, not It is disconnected to change subculture medium, until explant grows the high rooted seedlings of 7cm；The illumination carries out circulation light photograph using monochromatic light, Monochromatic circulation light is " tangerine lamp 30min- blue lamp 30min- red light 30min- green light 30min- tangerine lamps 30min " according to order；
(5) seedling is cultivated：The rooted seedling that step (4) obtains is put into the nutrition cup equipped with seedling culture medium to be put into greenhouse Cultivated, the greenhouse temperature is 28 DEG C, and illumination is solar radiation；During rooted seedling culture every 20d to rooted seedling according to Foliar fertilizer is sprayed in 90g/ strains；Under growth root height of seedling degree grows to 20cm and obtains pearl Lee seedling；
(2) pearl Lee plants：
A, readjustment of field plot：Pre- kind is ploughed, afterwards according to 4kg/m2Applied amount shed into pre- kind ground and kill stone Ashes is carried out disinfection, and the plot after offseting poison with mulch film is covered, and mulch film is opened after covering 40d, and carry out it is secondary plough, Then according to 3kg/m2Dose to after secondary plough soil apply organic fertilizer, ploughed three times again afterwards, and cover Mulch film, make compost 30d and obtain planting plot；
B, pearl Lee is planted：The cultured pearl Lee seedling of step (5) is colonized in step A according to 3m × 3m seeding row spacing Plantation plot in, according to 1200g/m after field planting2Dose apply organic liquid manure, afterwards at interval of 20d into plantation plot Apply a liquid manure, apply 12 times altogether；Planting process irregularly applies expelling parasite water to pearl Lee seedling, and keeps the phase of soil It is 40% to humidity.
Wherein, the indoor thimerosal of step (1) is made up of the composition of following percentage by weight：7% pepper extract, 7% Herba Fimbristylis dichotomae extract, 3% sodium hypochlorite, 3% sodium chloride, 3% calcium hydroxide, surplus are that percentage by volume is 95% ethanol solution.
(the present embodiment, the extracting method of pepper extract, the extracting method of Herba Fimbristylis dichotomae extract and interior in indoor thimerosal The collocation method of thimerosal and embodiment 1 are completely the same).
Wherein, the explant thimerosal of step (1) is made up of the composition of following percentage by weight：7% shrubby sophora extract, 7% capsicum extract, 7% garlic P.E, 5% chitosan, 5%-7% agastache extract surplus are volume basis Number is 75% ethanol solution.
(the present embodiment, it is the extracting method of shrubby sophora extract in explant thimerosal, the extracting method of pepper extract, big The extracting method of garlic extract, the collocation method of the extracting method of agastache extract and thimerosal and embodiment 1 are completely the same).
Wherein, the culture medium I of step (3) is made up of the composition of following percentage by weight：30% silkworm excrement soak, 7% Agar, 5% trehalose, 5% spirulina extract, 6% aloe extract, 3% magnesium sulfate, 3% sodium chloride, Surplus is water.
(the present embodiment, the immersion process of silkworm excrement soak, the extracting method of spirulina extract, aloe carry in culture medium I Take thing extracting method and culture medium I collocation method and embodiment 1 it is completely the same).
Wherein, the medium ii of step (4) is made up of the composition of following percentage by weight：50% silkworm excrement soak, 7% Agar, 5% trehalose, 5% Chinese toon leaf extract, 6% pepper extract, 3% magnesium sulfate, 3% biphosphate Sodium, surplus are water.
(the present embodiment, the immersion process of silkworm excrement soak, the extracting method of Chinese toon leaf extract, Chinese prickly ash carry in medium ii Take thing extracting method and medium ii collocation method and embodiment 1 it is completely the same).
Wherein, step (5) seedling culture medium is made up of the composition of following percentage by weight：35% beans shell, 25% flower Raw shell, 20% stalk, 15% loess and 5% organic fertilizer.
(the present embodiment, the preparation method and embodiment 1 of seedling culture medium are completely the same).
Wherein, the foliar fertilizer of step (5) is made up of the composition of following percentage by weight：40% urine dilution, 7% Pepper extract, 5% papaya extractives, 5% humic acid, surplus are water.
(the present embodiment, the collocation method of urine dilution, the extracting method of pepper extract, pawpaw extraction in foliar fertilizer The extracting method of thing and the collocation method of foliar fertilizer and embodiment 1 are completely the same).
Wherein, step A organic fertilizers comprise the following components in parts by weight：17 parts of lime stone, 34 parts of silkworm excrement, 17 parts Stalk, 27 parts of tea seed shell, 24 parts of Fructus Chaenomelis ferment and 22 parts of waste and old bacterium bag.
(the present embodiment, the preparation method of Fructus Chaenomelis ferment in organic fertilizer, waste and old bacterium bag source, organic fertilizer preparation method with Embodiment 1 is completely the same).
Wherein, the composition composition of the following percentage by weight of the organic liquid manure of step B：15% mulberry leaf ferment, 7% di(2-ethylhexyl)phosphate Hydrogen potassium, 7% nitric acid ammonia, 7% silkworm slough off extract, surplus is silkworm excrement soak that the content of organic matter is 8%.
(the present embodiment, the preparation method of mulberry leaf ferment, silkworm slough off the extracting method of extract, silkworm excrement immersion in organic liquid manure The immersion process of liquid, the collocation method of organic liquid manure and embodiment 1 are completely the same).
Wherein, the composition composition of the following percentage by weight of step B expelling parasites water：3% betelnut extract, 3% peppermint carry It is the sodium chloride that Solute mass percentage is 5% to take thing, 3% macrostachys uraria herb extract, 3% Andrographis Paniculata, surplus Solution.
(the present embodiment, the extracting method of betelnut extract, the extracting method of mint extract, macrostachys uraria herb carry in expelling parasite water Take extracting method, the extracting method of Andrographis Paniculata, the collocation method of expelling parasite water and the embodiment 1 of thing completely the same).
Embodiment 3：
A kind of implantation methods of pearl Lee, this method includes the culture of pearl Lee seedling and pearl Lee plants two steps：
(1) pearl Lee seedling is cultivated：
(1) culturing room sterilizes：7h is according to 500g/m before culture2Fountain height into the culturing room closed the doors and windows in spray booth Thimerosal carries out sterilization；
(2) explant pre-processes：Select the terminal bud of pearl Lee fruit tree and subtract unnecessary blade as explant, explant length For 2.5cm, a diameter of 0.5cm；Explant is rinsed with water, drain after be put into explant thimerosal and soaked, soak A length of 50min-60min obtains the explant pre-processed during bubble；
(3) explant culture：Step (2) pretreated explant is put into culture medium I and cultivated, cultivation temperature For 25 DEG C, intensity of illumination 1200Lx, light application time 14h, until explant grows sprouting；The explant and culture medium I Mass ratio be 1:27；The illumination carries out circulation light photograph using monochromatic light, and monochromatic circulation light is " red light according to order The white lamp 20min- green lights 20min- blue lamps 20min- red lights 20min " of 20min-；
(4) squamous subculture：The explant that sprouting is grown in step (3) is transferred and carries out squamous subculture in medium ii, Squamous subculture is carried out in squamous subculture case, cultivation temperature is 27 DEG C, intensity of illumination 1500Lx, light application time 22h, not It is disconnected to change subculture medium, until explant grows the high rooted seedlings of 6cm；The illumination carries out circulation light photograph using monochromatic light, Monochromatic circulation light is " tangerine lamp 30min- blue lamp 30min- red light 30min- green light 30min- tangerine lamps 30min " according to order；
(5) seedling is cultivated：The rooted seedling that step (4) obtains is put into the nutrition cup equipped with seedling culture medium to be put into greenhouse Cultivated, the greenhouse temperature is 24 DEG C, and illumination is solar radiation；During rooted seedling culture every 17d to rooted seedling according to Foliar fertilizer is sprayed in 80g/ strains；Under growth root height of seedling degree grows to 17cm and obtains pearl Lee seedling；
(2) pearl Lee plants：
A, readjustment of field plot：Pre- kind is ploughed, afterwards according to 3.5kg/m2Applied amount shed and kill into pre- kind ground Pulverized limestone is carried out disinfection, and the plot after offseting poison with mulch film is covered, and opens mulch film after covering 35d, and carry out secondary turn over Ground, then according to 2.5kg/m2Dose to after secondary plough soil apply organic fertilizer, ploughed three times again afterwards, and Mulch film is covered, 25d is made compost and obtains planting plot；
B, pearl Lee is planted：The cultured pearl Lee seedling of step (5) is existed according to 2.5m × 2.5m seeding row spacing field planting In step A plantation plot, according to 1000g/m after field planting2Dose apply organic liquid manure, afterwards at interval of 17d to plantation Apply a liquid manure in plot, apply 11 times altogether；Planting process irregularly applies expelling parasite water to pearl Lee seedling, and keeps soil The relative humidity of earth is 35%.
Wherein, the indoor thimerosal of step (1) is made up of the composition of following percentage by weight：6% pepper extract, 6% Herba Fimbristylis dichotomae extract, 2% sodium hypochlorite, 2% sodium chloride, 2% calcium hydroxide, surplus are that percentage by volume is 95% ethanol solution.
(the present embodiment, the extracting method of pepper extract, the extracting method of Herba Fimbristylis dichotomae extract and interior in indoor thimerosal The collocation method of thimerosal and embodiment 1 are completely the same).
Wherein, the explant thimerosal of step (1) is made up of the composition of following percentage by weight：6% shrubby sophora extract, 6% capsicum extract, 6% garlic P.E, 3% chitosan, 6% agastache extract surplus are that percentage by volume is 75% ethanol solution.
(the present embodiment, it is the extracting method of shrubby sophora extract in explant thimerosal, the extracting method of pepper extract, big The extracting method of garlic extract, the collocation method of the extracting method of agastache extract and thimerosal and embodiment 1 are completely the same).
Wherein, the culture medium I of step (3) is made up of the composition of following percentage by weight：25% silkworm excrement soak, 6% Agar, 3% trehalose, 4% spirulina extract, 4% aloe extract, 2% magnesium sulfate, 2% sodium chloride, Surplus is water.
(the present embodiment, the immersion process of silkworm excrement soak, the extracting method of spirulina extract, aloe carry in culture medium I Take thing extracting method and culture medium I collocation method and embodiment 1 it is completely the same).
Wherein, the medium ii of step (4) is made up of the composition of following percentage by weight：45% silkworm excrement soak, 6% Agar, 3% trehalose, 3% Chinese toon leaf extract, 4% pepper extract, 2% magnesium sulfate, 2% biphosphate Sodium, surplus are water.
(the present embodiment, the immersion process of silkworm excrement soak, the extracting method of Chinese toon leaf extract, Chinese prickly ash carry in medium ii Take thing extracting method and medium ii collocation method and embodiment 1 it is completely the same).
Wherein, step (5) seedling culture medium is made up of the composition of following percentage by weight：32% beans shell, 30% flower Raw shell, 25% stalk, 5% loess and 8% organic fertilizer.
(the present embodiment, the preparation method and embodiment 1 of seedling culture medium are completely the same).
Wherein, the foliar fertilizer of step (5) is made up of the composition of following percentage by weight：30%-40% urine dilution, 5%-7% pepper extract, 3%-5% papaya extractives, 3%-5% humic acid, surplus are water.
(the present embodiment, the collocation method of urine dilution, the extracting method of pepper extract, pawpaw extraction in foliar fertilizer The extracting method of thing and the collocation method of foliar fertilizer and embodiment 1 are completely the same).
Wherein, step A organic fertilizers comprise the following components in parts by weight：14 parts of lime stone, 28 parts of silkworm excrement, 15 parts Stalk, 19 parts of tea seed shell, 19 parts of Fructus Chaenomelis ferment and 19 parts of waste and old bacterium bag.
(the present embodiment, the preparation method of Fructus Chaenomelis ferment in organic fertilizer, waste and old bacterium bag source, organic fertilizer preparation method with Embodiment 1 is completely the same).
Wherein, the composition composition of the following percentage by weight of the organic liquid manure of step B：12% mulberry leaf ferment, 6% di(2-ethylhexyl)phosphate Hydrogen potassium, 6% nitric acid ammonia, 5% silkworm slough off extract, surplus is silkworm excrement soak that the content of organic matter is 7%.
(the present embodiment, the preparation method of mulberry leaf ferment, silkworm slough off the extracting method of extract, silkworm excrement immersion in organic liquid manure The immersion process of liquid, the collocation method of organic liquid manure and embodiment 1 are completely the same).
Wherein, the composition composition of the following percentage by weight of step B expelling parasites water：2% betelnut extract, 2% peppermint carry It is the sodium chloride that Solute mass percentage is 4% to take thing, 2% macrostachys uraria herb extract, 2% Andrographis Paniculata, surplus Solution.
(the present embodiment, the extracting method of betelnut extract, the extracting method of mint extract, macrostachys uraria herb carry in expelling parasite water Take extracting method, the extracting method of Andrographis Paniculata, the collocation method of expelling parasite water and the embodiment 1 of thing completely the same).
(1) control group of seedling processing
Control group 1：
This control group is identical with the breeding method of embodiment 1, but culturing room is carried out without using indoor thimerosal Sterilization, other methods are identical with embodiment 1.
Control group 2：
This control group is identical with the breeding method of embodiment 1, but explant is entered without using explant thimerosal Row sterilization, other methods are identical with embodiment 1.
Control group 3：
This control group is identical with the breeding method of embodiment 1, but explant is trained without using culture medium I Support, other methods are identical with embodiment 1.
Control group 4：
This control group is identical with the breeding method of embodiment 1, but explant is trained without using medium ii Support, other methods are identical with embodiment 1.
Control group 5：
This control group is identical with the breeding method of embodiment 1, but rooted seedling is carried out without using planted medium Culture, other methods are identical with embodiment 1.
Control group 6：
This control group is identical with the breeding method of embodiment 1, but sprays seedling, other methods without using foliar fertilizer It is identical with embodiment 1.
Control group 7：
By the direct seed of pearl Lee's seed in sandy soil earth, treat that seedling grows to 15cm and obtains pearl Lee seedling.
(2) control group of pearl Lee plantation：
Control group 8：
This control group is identical with the implantation methods of embodiment 1, but secondary during readjustment of field plot ploughs without using organic Fertilizer, other methods are identical with embodiment 1.
Control group 9：
This control group is identical with the implantation methods of embodiment 1, but plants pearl Lee and do not apply organic liquid manure, and makes It is identical with embodiment 1 with urea, other methods.
Control group 10：
This control group is identical with the implantation methods of embodiment 1, but plants pearl Lee and do not apply expelling parasite water, and uses Running water, other methods are identical with embodiment 1.
Control group 11：
The pearl Lee seedling cultivated using embodiment 1 is planted, but implantation methods use conventional method, that is, applies urine Element is sprayed insecticide as fertilizer and carries out expelling parasite.
Control group 12：
The pearl Lee seedling cultivated without using embodiment 1 is planted, i.e. children pearl Lee purchased in market is directly planted after ploughing Seedling, and apply nitrogen, spray insecticide.
Seedling experiment measurement：
Measure simultaneously Statistics Implementation example 1-3 and control group 1-6 explant survival rate, germination percentage, shoot survival percent of taking root, germination Rate, specifically it is shown in Table 1；Test seedling culture terminates the diameter of rear embodiment 1-3 and control group 1-7 pearl Lee's seedling trunk, treasure The growth time of pearl Lee seedling weight, the survival rate of pearl Lee seedling and pearl Lee seedling, is specifically shown in Table 2：
Table 1
As seen from the above table, the survival rate of embodiment 1-3 explant and rooted seedling, germination percentage are all higher than control group 1-6；Say Indoor thimerosal, explant thimerosal, culture medium I, medium ii, planted medium and the foliar fertilizer of bright the application can be effective Improve explant and survival rate, the germination percentage of rooted seedling.
Table 2
As seen from the above table, embodiment 1-3 trunk diameter, seedling weight, survival rate are all higher than control group 1-7, wherein, it is main The minimum control group 7 of dry diameter, seedling weight, survival rate；Embodiment 1-3 growth time is shorter than control group 1-7, wherein, when Between up to control group 7；It follows that the indoor thimerosal of the application, explant thimerosal, culture medium I, medium ii, kind Pearl Lee's seedling quality can be effectively improved by planting culture medium and foliar fertilizer.
(2) pearl Lee planting experiment：
Measure and to count embodiment 1-3 and control group 8-12 pearl Lee plant height after plantation 1 year, pearl Lee's blade complete Degree, pearl Lee's leaf total nitrogen content, content of tatal phosphorus, full potassium content, are specifically shown in Table 3：
Table 3
Group Plant height (cm) Blade integrity degree (%) Nitrogen content (mg/g) Phosphorus content (mg/g) Potassium content (mg/g)
Embodiment 1 142.36 99.64 305.21 64.25 51.26
Embodiment 2 140.52 99.06 312.05 68.54 50.64
Embodiment 3 143.25 99.65 309.54 69.25 51.36
Control group 8 121.01 97.99 202.86 42.60 31.72
Control group 9 119.17 97.41 209.70 42.71 30.77
Control group 10 121.90 88.58 300.80 58.92 48.82
Control group 11 110.66 96.34 200.51 40.95 30.18
Control group 12 101.82 91.76 189.35 31.38 24.90
As seen from the above table, embodiment 1-3 plant height, nitrogen content, phosphorus content, potassium content are all higher than control group 8-12, wherein Minimum is control group 12, illustrates to grow than the seedling that commercially available pearl Lee cultivating seeds come out using the seedling that the application cultivates Faster, seedling quality is higher；Embodiment 1-3 blade integrity degree and control group 8,9,11 quite apparently higher than control group 10,12, Effective anthelmintic action can be played by illustrating the pest repellant of the application.
In summary, planted using pearl Lee seedling of the present invention, and use pearl Lee's planting technology of the application The survival rate of pearl Lee can be effectively improved, the quality of pearl Lee fruit tree is improved, will not also cause environmental pollution.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (10)

1. a kind of implantation methods of pearl Lee, it is characterised in that this method includes the culture of pearl Lee seedling and pearl Lee plantation two Individual step：
(1) pearl Lee seedling is cultivated：
(1) culturing room sterilizes：6h-8h is according to 400g/m before culture2-600g/m2Fountain height sprayed into the culturing room closed the doors and windows Spill indoor thimerosal and carry out sterilization；
(2) explant pre-processes：Select the terminal bud of pearl Lee fruit tree and to subtract unnecessary blade a length of as explant, explant 2cm-3cm, a diameter of 0.4cm-0.6cm；Explant is rinsed with water, drain after be put into explant thimerosal and soaked Bubble, a length of 50min-60min obtains the explant pre-processed during immersion；
(3) explant culture：Step (2) pretreated explant is put into culture medium I and cultivated, cultivation temperature 24 DEG C -26 DEG C, intensity of illumination 1000Lx-1500Lx, light application time 12h-16h, until explant grows sprouting；The explant Body and culture medium I mass ratio are 1:25-30；The illumination carries out circulation light photograph using monochromatic light, and monochromatic circulation light shines Order is " the white lamp 20min- green lights 20min- blue lamps 20min- red lights 20min " of red light 20min-；
(4) squamous subculture：The explant that sprouting is grown in step (3) is transferred and carries out squamous subculture in medium ii, after For squamous subculture is carried out in incubator, cultivation temperature is 25 DEG C -28 DEG C, intensity of illumination 1300Lx-1700Lx, and light application time is 20h-24h, and subculture medium is constantly changed, until explant grows the high rooted seedlings of 5cm-7cm；The illumination is using monochromatic Light carries out circulation light photograph, and monochromatic circulation light is " tangerine lamp 30min- blue lamp 30min- red light 30min- green lights according to order 30min- tangerine lamps 30min "；
(5) seedling is cultivated：The rooted seedling that step (4) obtains is put into the nutrition cup equipped with planted medium be put into greenhouse and carry out Culture, the greenhouse temperature are 22 DEG C -28 DEG C, and illumination is solar radiation；Pressed during rooted seedling culture every 15d-20d to rooted seedling Foliar fertilizer is sprayed according to 70g/ strain -90g/ strains；Under growth root height of seedling degree grows to 15cm-20cm and obtains pearl Lee seedling；
(2) pearl Lee plants：
A, readjustment of field plot：Pre- kind is ploughed, afterwards according to 3kg/m2-4kg/m2Applied amount shed and kill into pre- kind ground Pulverized limestone is carried out disinfection, and the plot after offseting poison with mulch film is covered, and opens mulch film after covering 30d-40d, and carry out two It is secondary to plough, then according to 2kg/m2-3kg/m2Dose to after secondary plough soil apply organic fertilizer, carry out three again afterwards It is secondary to plough, and mulch film is covered, make compost 20d-30d and obtain planting plot；
B, pearl Lee is planted：The cultured pearl Lee seedling of step (5) is colonized in step according to 2m-3m × 2m-3m seeding row spacing In rapid A plantation plot, according to 800g/m after field planting2-1200g/m2Dose apply organic liquid manure, afterwards at interval of 15d- 20d applies a liquid manure into plantation plot, applies 9 times -12 times altogether；Planting process irregularly applies to pearl Lee seedling drives Worm water, and it is 30%-40% to keep the relative humidity of soil.
2. the implantation methods of a kind of pearl Lee according to claim 1, it is characterised in that the interior of the step (1) disappears Venom is made up of the composition of following percentage by weight：5%-7% pepper extract, 5%-7% Herba Fimbristylis dichotomae extract, 1%- 3% sodium hypochlorite, 1%-3% sodium chloride, 1%-3% calcium hydroxide, surplus are the ethanol that percentage by volume is 95% Solution.
A kind of 3. implantation methods of pearl Lee according to claim 1, it is characterised in that the explant of the step (1) Thimerosal is made up of the composition of following percentage by weight：5%-7% shrubby sophora extract, 5%-7% capsicum extract, 5%- 7% garlic P.E, 2%-5% chitosan, 5%-7% wrinkled giant hyssop extract solution, surplus are that percentage by volume is 75% Ethanol solution.
A kind of 4. implantation methods of pearl Lee according to claim 1, it is characterised in that the culture medium I of the step (3) It is made up of the composition of following percentage by weight：20%-30% silkworm excrement soak, 5%-7% agar, 2%-5% marine alga Sugar, 2%-5% spirulina extract, 3%-6% aloe extract, 1%-3% magnesium sulfate, 1%-3% sodium chloride, Surplus is water.
A kind of 5. implantation methods of pearl Lee according to claim 1, it is characterised in that the culture medium of the step (4) II is made up of the composition of following percentage by weight：40%-50% silkworm excrement soak, 5%-7% agar, 2%-5% sea Algae sugar, 2%-5% Chinese toon leaf extract, 3%-6% pepper extract, 1%-3% magnesium sulfate, 1%-3% di(2-ethylhexyl)phosphate Hydrogen sodium, surplus are water.
A kind of 6. implantation methods of pearl Lee according to claim 1, it is characterised in that step (5) the plantation culture Base is made up of the composition of following percentage by weight：30%-35% beans shell, 20%-30% peanut shell, 20%-30% straw The loess of stalk, 5%-15%, 5%-10% organic fertilizer.
A kind of 7. implantation methods of pearl Lee according to claim 1, it is characterised in that the foliar fertilizer of the step (5) It is made up of the composition of following percentage by weight：30%-40% urine dilution, 5%-7% pepper extract, 3%-5% Papaya extractives, 3%-5% humic acid.
8. the implantation methods of a kind of pearl Lee according to claim 1, it is characterised in that the step A organic fertilizers are by such as The composition composition of lower parts by weight：13 parts -17 parts of lime stone, 23 parts -34 parts of silkworm excrement, 12 parts -17 parts of stalk, 17 part -27 The tea seed shell, 12 parts -24 parts of Fructus Chaenomelis ferment and 14 parts -22 parts of waste and old bacterium bag of part.
9. the implantation methods of a kind of pearl Lee according to claim 1, it is characterised in that the organic liquid manure of step B is such as The composition composition of lower percentage by weight：10%-15% mulberry leaf ferment, 5%-7% potassium dihydrogen phosphate, 5%-7% nitric acid Ammonia, 2%-7% silkworm slough off extract, surplus is silkworm excrement soak that the content of organic matter is 5%-8%.
10. the implantation methods of a kind of pearl Lee according to claim 1, it is characterised in that the step B expelling parasite water is as follows The composition composition of percentage by weight：1%-3% betelnut extract, 1%-3% mint extract, 1%-3% macrostachys uraria herb Extract, 1%-3% Andrographis Paniculata, surplus are the sodium chloride solution that Solute mass percentage is 3%-5%.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109122200A (en) * 2018-08-24 2019-01-04 云南省农业科学院农业环境资源研究所 A kind of Paris polyphylla cultivation disease-resistant matrix of water conservation and preparation method thereof
CN109220449A (en) * 2018-08-31 2019-01-18 贺州市飞龙种养专业合作社 The implantation methods of slow seedling are taken root based on quickening plum
CN112189564A (en) * 2020-11-03 2021-01-08 毕节市林业科学研究所 Method for propagating betula luminifera aseptic seedlings by tissue culture technology

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CN105284533A (en) * 2015-10-30 2016-02-03 天峨县科学技术情报研究所 Cultivation method for Longtan pearl-shaped plum tree
CN105961205A (en) * 2016-07-28 2016-09-28 广西陆川县乌坭坡珍珠番石榴专业合作社 Tissue culture method for increasing survival rate of psidium guajava L.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105284533A (en) * 2015-10-30 2016-02-03 天峨县科学技术情报研究所 Cultivation method for Longtan pearl-shaped plum tree
CN105961205A (en) * 2016-07-28 2016-09-28 广西陆川县乌坭坡珍珠番石榴专业合作社 Tissue culture method for increasing survival rate of psidium guajava L.

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109122200A (en) * 2018-08-24 2019-01-04 云南省农业科学院农业环境资源研究所 A kind of Paris polyphylla cultivation disease-resistant matrix of water conservation and preparation method thereof
CN109220449A (en) * 2018-08-31 2019-01-18 贺州市飞龙种养专业合作社 The implantation methods of slow seedling are taken root based on quickening plum
CN112189564A (en) * 2020-11-03 2021-01-08 毕节市林业科学研究所 Method for propagating betula luminifera aseptic seedlings by tissue culture technology

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Application publication date: 20180327