CN105659969B - A kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy - Google Patents
A kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy Download PDFInfo
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- CN105659969B CN105659969B CN201610063985.1A CN201610063985A CN105659969B CN 105659969 B CN105659969 B CN 105659969B CN 201610063985 A CN201610063985 A CN 201610063985A CN 105659969 B CN105659969 B CN 105659969B
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- harmel
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- cauline leaf
- ammopiptanthus mongolicus
- culture dish
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- 240000005523 Peganum harmala Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 26
- 241000586291 Ammopiptanthus mongolicus Species 0.000 title claims abstract description 24
- 230000036531 allelopathy Effects 0.000 title claims abstract description 14
- 230000007226 seed germination Effects 0.000 title claims abstract description 13
- 238000002386 leaching Methods 0.000 claims abstract description 19
- 238000001802 infusion Methods 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 6
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 238000007873 sieving Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000011218 seed culture Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000012153 distilled water Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 230000035784 germination Effects 0.000 abstract description 15
- 230000012010 growth Effects 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 4
- 238000003809 water extraction Methods 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 18
- 238000011160 research Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000012545 processing Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241000282836 Camelus dromedarius Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000029602 competition with other organism Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention discloses a kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy, it comprises the following steps：First, material to be tested is handled：Harmel drying, crush, sieving；2nd, prepared by leaching liquor：Water extraction+ultrasonic-leaching+water extraction+centrifugation；3rd, seed culture：Harmel cauline leaf infusion is injected into the culture dish for having sowed Ammopiptanthus mongolicus seed, is placed in intelligent growth cabinet and is cultivated after culture dish capping, temperature is 25 DEG C of daytime, 20 DEG C of night, humidity 75%, illumination 12h, light intensity 8000LX.The present invention is advantageous in that：(1) cost is low；(2) germination rate is high, in the range of 83% 89% (control group 58%)；(3) speed of germination is fast, and mean germination index is in 61.5% 66% (control groups 53%)；(4) it is environmentally safe without using chemicals.
Description
Technical field
The present invention relates to a kind of method for improving seed germination rate, and in particular to one kind is improved using harmel allelopathy
The method of Ammopiptanthus mongolicus seed germination rate, belongs to breeding technical field.
Background technology
The allelopathy (Alelopathy) of plant refers to that growing plants or its litter pass through a variety of in the ecosystem
Approach is to Environment release particular chemicals so as to promoting or suppress itself or the effect of neighbouring plant seed germination and growth.
Allelopathy has turned into the important scientific issues of ecological study between plant at present, and research field focuses primarily upon
Alien species, the succession of community, genetic breeding and agriculture and forestry production etc., and obtained the achievement attracted attention；The region of research
Scope is mainly in coastal, temperate zone and frigid zone grassland ecological；Floristics with crops, forest plants, flowers, herbage, medicinal material and
Based on water plant, the research of allelopathy is rarely reported abroad between the plant of China arid-desert area, but does not cause state also
The attention of interior scholar.
Research shows that in severe habitat plant itself often increases the release concentration of metabolite and had stronger
Allelopathic effects.
Therefore, ermophyte of the long term growth in barren soil, awful weather habitat is the change of more worth further investigation
Feel plant resources.
The content of the invention
It is an object of the invention to provide it is a kind of using harmel allelopathy improve Ammopiptanthus mongolicus seed germination rate method,
Germination rate of this method except Ammopiptanthus mongolicus seed can be effectively improved, moreover it is possible to improve its speed of germination.
In order to realize above-mentioned target, the present invention adopts the following technical scheme that：
A kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy, it is characterised in that including following
Step：
First, material to be tested is handled：
(1) harmel plant is won into cauline leaf, cleaned, air drying and stoving to constant weight, crushed, be placed in 4 DEG C of low temperature after sieving
Lower sealing is stand-by；
(2) select uniform, full, of the same size Ammopiptanthus mongolicus seed, carry out successively clear water flushing-warm water seed soaking-
HgCl2Immersion-distilled water flushing, then it is seeded in stand-by in the culture dish for be covered with double-layer filter paper；
2nd, prepared by leaching liquor：
Harmel cauline leaf powder is placed in distilled water, soaked at room temperature, active matter is extracted using ultrasonic-leaching method afterwards
Matter, extract at 40 DEG C, will be centrifuged afterwards after leaching liquor double filtration again, supernatant is harmel cauline leaf leaching liquor, adjusts white horse with a black mane
The concentration of the fluffy cauline leaf leaching liquor of camel is in 5-15mgml-1In the range of；
3rd, seed culture：
Harmel cauline leaf infusion is injected into the culture dish for having sowed Ammopiptanthus mongolicus seed, intelligence is placed in after culture dish capping
Cultivated in growth cabinet, temperature is 25 DEG C of daytime, 20 DEG C of night, humidity 75%, illumination 12h, light intensity 8000LX.
Foregoing method, it is characterised in that in step 1, the drying temperature of harmel is 70-85 DEG C.
Foregoing method, it is characterised in that in step 1, the aperture of sieve is 1mm.
Foregoing method, it is characterised in that in step 2, a length of 48h when harmel cauline leaf powder soaks at room temperature.
Foregoing method, it is characterised in that during soaking at room temperature, 10min is shaken every 12h.
Foregoing method, it is characterised in that in step 2, a length of 30min when harmel cauline leaf powder soaks at 40 DEG C.
Foregoing method, it is characterised in that in step 3, after starting culture, sop up culture dish with rubber head dropper daily
In unnecessary liquid, and supplement the infusion of appropriate corresponding concentration.
The present invention is advantageous in that：(1) cost is low；(2) germination rate is high, and (control group is in the range of 83%-89%
58%)；(3) speed of germination is fast, and mean germination index is at 61.5%-66% (control group 53%)；(4) without using chemical drugs
Product, it is environmentally safe.
Embodiment
Angle research plant stem-leaf infusion of the invention from Allelopathic Effect in Plants is to husky raw endangered plants Ammopiptanthus mongolicus seed
Sprouting and the influence of growth of seedling, illustrate its allelopathy mechanism, it is intended to the interaction probed between harmel and other species
Relation, while an angle thinking Desert Regions Species structure lattice beyond the external condition of plant growth can be more fully provided
The method for the problem of office, interspecies competition mechanism, succession of community rule etc. are all many-sided.
The present invention not only helps abundant STUDY ON ALLELOPATHIC EFFECTS theory, and to instructing area's Vegetation Construction that there is important meaning
Justice.
Make specific introduce to the present invention below in conjunction with specific embodiment.
The first step：Material to be tested processing
Harmel plant used in experiment and Ammopiptanthus mongolicus seed pick up from Shiyanghe River downstream respectively in September And October, 2015.
After harmel plant is taken back into laboratory, win cauline leaf and cleaned with distilled water, be positioned at 80 DEG C and dry after drying
Do to constant weight, crush, be placed in after sieving (aperture 1mm) under 4 DEG C of low temperature seal it is stand-by.
Uniform, full, of the same size Ammopiptanthus mongolicus seed is selected, first seed is rinsed well with clear water, then uses 55-60
DEG C warm water seed soaking 15min, then with 0.1% HgCl23min is soaked, rinses 5-6 times repeatedly with distilled water (to wash residual off
HgCl2) after be seeded in the culture dish for being covered with double-layer filter paper (culture dish and filter paper do high-temperature disinfection treatment), put 50 per ware
Seed, it is stand-by.
Second step：It is prepared by leaching liquor
The harmel cauline leaf powder 20g after crushing, sieving is weighed, is placed in 500ml beakers, 200ml distilled water is added, with guarantor
Fresh film sealing simultaneously soaks 48h (shaking 10min every 12h during this) under room temperature (20-25 DEG C), afterwards using ultrasonic-leaching
Method extracts active material, extracts 30min at 40 DEG C, (will first be filtered afterwards after leaching liquor double filtration with quantitative filter paper, then with filtering
Unit is 0.45um filtering with microporous membrane) 15min is centrifuged under 6500r, gained supernatant is that concentration is 100mgml-1's
Harmel cauline leaf leached liquor, it is placed in 4 DEG C of refrigerators and preserves.
In use, leaching liquor is diluted to 5 respectively, 15,25,50mgml-1It is standby.
3rd step：Seed culture
The harmel cauline leaf infusion 10ml of various concentrations is injected separately into the culture dish for having sowed Ammopiptanthus mongolicus seed (to be made
Infusion is first placed to a period of time at room temperature with preceding, in order to avoid quenching produces injury to Ammopiptanthus mongolicus seed), control adds 10ml
Distilled water, often processing set 3 repetitions, and (the RQX-400H Shanghai leap medical treatment of intelligent growth cabinet is placed in after culture dish capping
Apparatus Co., Ltd) in cultivated, temperature be 25 DEG C of daytime, 20 DEG C of night, humidity 75%, illumination 12h, light intensity 8000LX.
After starting culture, liquid unnecessary in culture dish is sopped up with rubber head dropper daily, and supplements the water of 1ml corresponding concentrations
Immersion liquid or distilled water, to keep leaching liquor concentration and water regime in culture dish constant.
Daily observational record germination number (the prominent 1mm that breaks in the seed coat of radicle is considered as sprouting) is simultaneously chosen except chitting piece, continuous 3d
There is no off-test during new seed germination.
4th step：Calculate germination percentage
Germination percentage (%)=(subnumber is planted experimentally in chitting piece number/confession) × 100%
Germination index GI=∑s (Gt/Dt)
In formula, GtRefer to the seed number germinateed within t days；DtRefer to corresponding germination number of days.
In general, allelopathic plant infusion sprouts the phenomenon with " low to promote high suppression " to recipient plant, and this phenomenon exists
Showed in Ammopiptanthus mongolicus Seed Germination very prominent：
Ammopiptanthus mongolicus seed germination rate is 58% during control treatment, with 5,15,25,50mgml-1The leaching liquor of various concentrations
During processing, its germination rate is respectively 89%, 83%, 71%, 44%, and the mean germination index of seed is relatively compareed also with cauline leaf
The rise of infusion concentration and reduce, its value is respectively 53% (control), 66%, 61.5%, 43%, 23%.
This phenomenon explanation, the harmel cauline leaf infusion of low concentration can not only significantly improve the seed sprouting of Ammopiptanthus mongolicus
Rate, and the speed sprouted to Ammopiptanthus mongolicus seed also has significant quickening to act on.
As can be seen here, method of the invention can not only significantly improve the seed germination rate of Ammopiptanthus mongolicus, but also can show
The speed accelerated Ammopiptanthus mongolicus seed and sprouted of work.
Further, since the present invention has mainly used harmel leaching liquor, so not only breeding cost is low, and without using change
Medicine is learned, it is environmentally safe, there is fabulous application value.
Because arid-desert area growth and development of plants and distribution are influenceed by a variety of severe extraneous factors, and biochemical substances
It is the important factor for influenceing natural plant renewal, conventional people are for the arid-desert area plant regeneration that is grown under severe habitat
Success or failure are often more from the consideration of the external conditions such as the illumination residing for seed and seedling, moisture, nutrient, and to as renewal success or failure
Latent factor --- allelopathy is underestimated.Allelopathy be prevalent in plant inter-species, kind in, with regard to desertification control
For field, reasonable application will produce good effect.Therefore, pay attention to and carry out the research of ermophyte allelopathy, no
But there is directive function for explaining arid-desert area plant life mechanism and implementing revegetation, and to area agricultural and life
The reasonable improvement and sustainable development of state safety are significant.
It should be noted that the invention is not limited in any way for above-described embodiment, it is all to use equivalent substitution or equivalent change
The technical scheme that the mode changed is obtained, all falls within protection scope of the present invention.
Claims (7)
- A kind of 1. method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy, it is characterised in that including following step Suddenly：First, material to be tested is handled：(1) harmel plant is won into cauline leaf, cleaned, air drying and stoving to constant weight, is placed in after crushing, sieving close under 4 DEG C of low temperature Seal stand-by；(2) uniform, full, of the same size Ammopiptanthus mongolicus seed is selected, carries out clear water flushing-warm water seed soaking-HgCl successively2Leaching Bubble-distilled water flushing, then it is seeded in stand-by in the culture dish for be covered with double-layer filter paper；2nd, prepared by leaching liquor：20g harmel cauline leaf powder is placed in 200ml distilled water, soaked at room temperature, is extracted live using ultrasonic-leaching method afterwards Property material, extract at 40 DEG C, will be centrifuged afterwards after leaching liquor double filtration, gained supernatant is that concentration is 100mgml again-1's Harmel cauline leaf leached liquor, the concentration of harmel cauline leaf leaching liquor is adjusted during use in 5-15mgml-1In the range of；3rd, seed culture：Harmel cauline leaf infusion is injected into the culture dish for having sowed Ammopiptanthus mongolicus seed, it is artificial to be placed in intelligence after culture dish capping Cultivated in climate box, temperature is 25 DEG C of daytime, 20 DEG C of night, humidity 75%, illumination 12h, light intensity 8000LX.
- 2. according to the method for claim 1, it is characterised in that in step 1, the drying temperature of harmel is 70-85 ℃。
- 3. according to the method for claim 1, it is characterised in that in step 1, the aperture of sieve is 1mm.
- 4. according to the method for claim 1, it is characterised in that in step 2, harmel cauline leaf powder soaks at room temperature Shi Changwei 48h.
- 5. according to the method for claim 4, it is characterised in that during soaking at room temperature, shaken every 12h 10min。
- 6. according to the method for claim 1, it is characterised in that in step 2, harmel cauline leaf powder soaks at 40 DEG C Shi Changwei 30min.
- 7. according to the method for claim 1, it is characterised in that in step 3, after starting culture, use rubber head dropper daily Liquid unnecessary in culture dish is sopped up, and supplements the infusion of appropriate corresponding concentration.
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| CN201610063985.1A CN105659969B (en) | 2016-01-29 | 2016-01-29 | A kind of method that Ammopiptanthus mongolicus seed germination rate is improved using harmel allelopathy |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106613424A (en) * | 2017-01-18 | 2017-05-10 | 云南农业大学 | Micro-plant hydrotrope allelopathic potential evaluation bioassay method |
| CN106717220A (en) * | 2017-02-15 | 2017-05-31 | 兰州大学 | The method for regulating and controlling psilium succession with litter extract solution |
| CN107912455B (en) * | 2017-11-30 | 2021-02-05 | 融安县浪溪河谷金桔开发有限责任公司 | Treatment method for improving germination rate of annual fleabane seeds |
| CN109169120B (en) * | 2018-09-10 | 2021-05-25 | 天津农学院 | Allelopathy-based leguminous arbor and grass composite planting method and verification method thereof |
| CN109953043B (en) * | 2019-04-28 | 2021-03-30 | 福建农林大学 | Inhibitor for growth of associated variety of Chinese fir based on Grignard tannin extract litter leaching liquor |
| CN109953042B (en) * | 2019-04-28 | 2021-03-30 | 福建农林大学 | Inhibitor for growth of acacia taiwan based on leaching liquor of castanopsis gracilis litter |
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| CN103960040A (en) * | 2014-05-31 | 2014-08-06 | 中国科学院新疆生态与地理研究所 | Seedling cultivation method of ammopiptanthus nanus in desert |
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| JPH05284849A (en) * | 1992-04-08 | 1993-11-02 | Tetsuya Kishino | Mixed seed group of grass |
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| CN102612968A (en) * | 2012-04-09 | 2012-08-01 | 天津师范大学 | Ammopiptanthus introducing and cultivating method |
| CN103960040A (en) * | 2014-05-31 | 2014-08-06 | 中国科学院新疆生态与地理研究所 | Seedling cultivation method of ammopiptanthus nanus in desert |
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