CN103749205B - A kind of rhizosphere soil is separated and the plant roots bag Synthetical cultivation device collected - Google Patents

A kind of rhizosphere soil is separated and the plant roots bag Synthetical cultivation device collected Download PDF

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Publication number
CN103749205B
CN103749205B CN201410018706.0A CN201410018706A CN103749205B CN 103749205 B CN103749205 B CN 103749205B CN 201410018706 A CN201410018706 A CN 201410018706A CN 103749205 B CN103749205 B CN 103749205B
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soil
cylindrical shell
mesh
water pipe
cylinder
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CN103749205A (en
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牛晓君
李丽
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South China University of Technology SCUT
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South China University of Technology SCUT
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Abstract

The invention discloses a kind of rhizosphere soil to be separated and the plant roots bag Synthetical cultivation device collected, it is characterized in that, comprise a cylindrical shell, be provided with rack at cylinder body bottom, filter screen placed by rack, cylindrical shell is divided into the soil district on top and the storage area of bottom by filter screen; The middle part in soil district is provided with root landing net cylinder, and soil division is divided into centre chamber and annular edge room by this net cylinder; The outside being close to net cylinder is provided with the circular inserted sheet of a mesh or at least two circular inserted sheets of the mesh be close to successively; The cylindrical shell of storage area is provided with delivery port, extends to form a locating slot by this delivery port to the cylinder lateral wall above it, and be provided with transparent water pipe in described locating slot, water pipe is marked with graduation mark, and the lower port of water pipe is communicated with the delivery port of cylindrical shell.Adopt device of the present invention can realize easily and accurately being separated the soil of rhizosphere different mm level and collecting.

Description

A kind of rhizosphere soil is separated and the plant roots bag Synthetical cultivation device collected
Technical field
The present invention relates to a kind of plant roots bag culture apparatus, particularly a kind of plant roots bag Synthetical cultivation device being separated for rhizosphere soil and collecting.
Background technology
Rhizosphere refers to that root-Soil Interface arrives the microbending soil of several millimeters of scopes less than 1 millimeter, is also plant-soil-microorganism and the interactional place of its environmental condition.Due to the effect of Gen Mao and root secretion, and microbial activities stronger within the scope of rhizosphere, make the characteristic of the aspects such as rhizosphere soil physics, chemistry and biology obviously be different from soil body soil.Thus, the nutrient situation of plant rhizosphere soil more can reflect validity to plant than the whole soil body.Therefore, the Nutrient Environment of rhizosphere and soil microbial activity are the study hotspots in plant nutrition field always.The experimental results shows, due to varying in size of various nutrient removal in the absorption rate of plant and soil, the Nutrient distribution in rhizosphere soil is inhomogenous.For separation and the collection method of rhizosphere soil, tradition research adopts artificial dithering and root bag method.There is the inhomogenous defect of sampling amount in these two kinds of methods, and easily cause minority root system to organize the residual of especially root hair under the conditions such as different floristics, soil types and field humidity.The method of current research rhizospheric environment mainly contains radioautography, microbending soil peels off partition method, Frozen sections, microelectrode method and electron probe method etc.Corresponding defect is always there is in collection rhizosphere soil in these methods in separation.In order to obtain the soil specimen from root table different distance, people devise square frame three Room root box culturing device.But not easily the tightening every root net of this device, making troubles to fetching earth in unloading process, more or less destroying Rhizosphere Soil, error is larger.And this device can not with the air circulation in the external world, make plant root can not obtain the chance absorbing nutrient in air.In addition, square frame three Room root box culturing device causes the heterogeneity of pouring water because of the soil split difference of different root room, and due to the closed-loop layout of bottom, make user not only cannot learn the height of device water level inside, when running into when raining heavyly, also cannot unnecessary moisture be regulated, so dead because retaining too much causes plant root to rot, have impact on the carrying out of test.
Summary of the invention
The technical problem to be solved in the present invention is that providing a kind of is separated and the plant roots bag Synthetical cultivation device collected for rhizosphere soil, and arranging of root bagging apparatus can sample the soil from root different distance easily and accurately.Adopt device of the present invention can not only be circulated by air-vent and outside air, also there is the space of water storage, and also can facilitate its water level inside height of observation at any time, and more have convenient adjustment water level function, improve the survival rate of plant.
Object of the present invention is achieved through the following technical solutions:
Rhizosphere soil is separated and the plant roots bag Synthetical cultivation device collected, and comprise a cylindrical shell, be provided with rack at cylinder body bottom, filter screen placed by rack, cylindrical shell is divided into the soil district on top and the storage area of bottom by filter screen; The middle part in soil district is provided with root landing net cylinder, and soil division is divided into centre chamber and annular edge room by this net cylinder; The outside being close to net cylinder is provided with the circular inserted sheet of a mesh or at least two circular inserted sheets of the mesh be close to successively; The cylindrical shell of storage area is provided with delivery port, extends to form a locating slot by this delivery port to the cylinder lateral wall above it, and be provided with transparent water pipe in described locating slot, water pipe is marked with graduation mark, and the lower port of water pipe is communicated with the delivery port of cylindrical shell.
Above-mentioned filter screen is metal, plastic material or sponge material.
The circular inserted sheet of described mesh is made up of bracing frame and the net adhering to bracing frame side, and the thickness of bracing frame is 1mm.
Net on the circular inserted sheet of described landing net cylinder and mesh is the nylon wire of mesh diameter 25 microns.
The cylindrical shell in described soil district is provided with 4 air-vents, and wraps cylindrical shell with two-layer bundle.
The water outlet of described cylindrical shell is provided with a three-way pipe, and a mouth of pipe connects storage area, and a mouth of pipe connects transparent water pipe, and the remaining mouth of pipe is discharge outlet.
Rhizosphere soil sampling method: take out cylindrical center's room (i.e. phytotron), and the circular inserted sheet of the mesh taking out diverse location place in annular edge room, collect the soil specimen wherein carried, can reach the object of rhizosphere different mm level soil layer separated and collected.
A kind of rhizosphere soil of the present invention is separated and the plant roots bag Synthetical cultivation device collected, and has the following advantages and beneficial effect:
1, the structure owing to there being plastic water pipe to be embedded at this cylinder outer rim locating slot, and the graduation mark on it, and plastic water pipe is communicated with the storage area of inner barrel, too much moisture can be discharged through this plastic water pipe, and the height of water level inside can be observed intuitively.
2, the present invention adopts on capillary and inhales irrigation method, and the plastic water pipe namely passing through peripheral hardware, to storage area water filling, makes the moisture of inner barrel storage area be able to nature volatilization and rises, contribute to the moistening of soil; Solving pours water from top causes showing soil crust, unequal problem of pouring water.
3, because cylindrical shell has storage area, to plant from top fertilising process, can by the unnecessary moisture collection discharging fertilizer, recycling.
4, owing to being installed with 4 air-vents on cylindrical shell, air can enter soil gap easily, has both been conducive to the aerobic respiration of root, also can suppress the activity of denitrifying bacteria, prevents nitrogenous fertilizer denitrification loss.
5, by adopting the circular inserted sheet of the mesh of varying number, required rhizosphere soil can be obtained very easily, enormously simplify rhizosphere soil and being separated and the Methods and steps collected.
Accompanying drawing explanation
Fig. 1 is the generalized section of apparatus of the present invention using state;
Fig. 2 is the perspective view of Fig. 1;
Fig. 3 is the structural representation of root landing net cylinder;
Fig. 4 is the structural representation of the circular inserted sheet bracing frame of mesh.
Embodiment
With reference to accompanying drawing, the specific embodiment of the present invention is described in detail.
As shown in Figure 1, 2, give a kind of rhizosphere soil to be separated and the plant roots bag Synthetical cultivation device collected, comprise a cylindrical shell 1, bottom cylindrical shell 1, be provided with rack 2, rack 2 is placed filter screen 4, cylindrical shell 1 is divided into the soil district 11 on top and the storage area 12 of bottom by filter screen 4; The middle part in soil district is provided with root landing net cylinder 3, and soil division is divided into cylindrical center room 5 and annular edge room 6 by this net cylinder 3; The circular inserted sheet 7 of mesh in annular edge room 6 is seamlessly close to adjacent net cylinder 3, and the circular inserted sheet 7 of all meshes is seamlessly close to each other; The cylindrical shell of storage area is provided with delivery port 10, extends to form a locating slot 8 by this delivery port to the cylinder lateral wall above it, and be provided with transparent plastic water pipe 9 in described locating slot 8, water pipe is marked with graduation mark.The lower port of water pipe 9 is communicated with the delivery port 10 of cylindrical shell.The cylindrical shell in described soil district is provided with 4 air-vents 13, and wraps cylindrical shell with two-layer bundle.
Cylindrical shell 1 is processed into by PVC board, and thickness is 5mm, and root landing net cylinder 3 can take out, with facilitate plant cultivating and plant terminate cultivate time sampling operation.
As shown in Figure 4, the circular inserted sheet 7 of this mesh is made up of with the net adhering to bracing frame 71 side (upper lower bottom part is without net) the bracing frame 71 of interior sky, this net to be mesh diameter be nylon wire of 25 microns, this nylon wire is very thin, its thickness can be ignored, and reaches the circular inserted sheet 7 of each mesh and root landing net cylinder 3 object be close to of gapless each other when not affecting actual use.Bracing frame is made up of the epoxy resin that 1mm is thick, hard and the coefficient of expansion of epoxy resin quality is much larger than nylon wire, therefore can guarantee the accuracy of the soil thickness of the online carrying of the circular inserted sheet 7 of mesh under any damp condition, so just strictly can control the thickness size of each target soil layer 1mm in rhizosphere micro-region.
The circular quantity needed for inserted sheet 7 of mesh, fixed according to actual conditions.Such as need to gather according to the rhizosphere soil at root table 4mm place, then need the circular inserted sheet 7 of eye of throwing the net at inner close fitting root landing net cylinder 3 place, annular edge room 6 successively parallel insertion 3, this 3 throw the net eye circle inserted sheet 7 each other gapless be close to.During sampling, the on root landing net cylinder 3 direction the 3rd soil sample of carrying in the circular inserted sheet 7 of eye of throwing the net is the rhizosphere soil at distance root table 4mm place in rhizosphere micro-region.
When this rack 2 is placed in the bottom of this cylindrical shell 1, bottom it, a storage area 12 can be separated out in place.Filter screen 4 is the object generally with impurity screening, and aperture is 100 microns, is flat on above this rack 2.
Plant roots bag Synthetical cultivation device of the present invention, actual use procedure is as follows:
First, add appropriate water (being watched by the transparent plastic water pipe 9 in locating slot 8) in the bottom of cylindrical shell 1, then put into rack 2 and filter screen 4, then put into root landing net cylinder 3 and the circular inserted sheet 7 of mesh.Calculate when identical dress soil height, the soil of using of cylindrical center room 5 and annular edge room 6 is measured simultaneously, and soil needs to grind in advance to sieve.Soil is respectively charged into cylindrical center room 5 and annular edge room 6 according to corresponding by soil amount, and for preventing nylon wire by native crimp, the soil layering of two rooms loads, and after installing, the soil in cylindrical center room 5 and annular edge room 6 should be in sustained height.Plant into seedling in cylindrical center room 5, root system of plant in cylindrical center room 5 starts to stretch, and owing to being subject to the obstruct that aperture is the root landing net cylinder 3 of 25 microns, root system can be assembled on root landing net cylinder 3, therefore root system can only be positioned at cylindrical center room 5, and can not enter in annular edge room 6.Namely root landing net cylinder 3 becomes Root-soil interface, and the soil contacted with root landing net cylinder 3 in cylindrical center room 5 is rhizosphere soil.
This plastic water pipe 9 is utilized to be embedded at the structure of this cylindrical shell 1 outer rim locating slot 8, and the graduation mark on it, the height of water level inside can be observed intuitively.And rain when outdoor, or when moisture is too many, then unnecessary moisture in this storage area 12 can be discharged, promote convenience and the practicality of planting plants.
When needs carry out rhizosphere soil sampling, take out cylindrical center room 5(and phytotron), and the circular inserted sheet 7 of the mesh taking out diverse location place in annular edge room 6, collect the soil specimen wherein carried, the object of rhizosphere different mm level soil layer separated and collected can be reached.
Below be only a specific embodiment of the present invention.The invention is not restricted to above embodiment, many distortion can also be had.

Claims (5)

1. rhizosphere soil is separated and the plant roots bag Synthetical cultivation device collected, and it is characterized in that, comprise a cylindrical shell, be provided with rack at cylinder body bottom, filter screen placed by rack, cylindrical shell is divided into the soil district on top and the storage area of bottom by filter screen; The middle part in soil district is provided with root landing net cylinder, and soil division is divided into centre chamber and annular edge room by this net cylinder; The outside being close to net cylinder is provided with the circular inserted sheet of a mesh or at least two circular inserted sheets of the mesh be close to successively; The cylindrical shell of storage area is provided with delivery port, extends to form a locating slot by this delivery port to the cylinder lateral wall above it, and be provided with transparent water pipe in described locating slot, water pipe is marked with graduation mark, and the lower port of water pipe is communicated with the delivery port of cylindrical shell.
2. device according to claim 1, is characterized in that, the circular inserted sheet of described mesh is made up of bracing frame and the net adhering to bracing frame side.
3. device according to claim 1 and 2, is characterized in that, the net on the circular inserted sheet of described landing net cylinder and mesh is the nylon wire of mesh diameter 25 microns.
4. device according to claim 1 and 2, is characterized in that, the cylindrical shell in described soil district is provided with 4 air-vents, and wraps cylindrical shell with two-layer bundle.
5. device according to claim 1 and 2, is characterized in that, the water outlet of described cylindrical shell is provided with a three-way pipe, and a mouth of pipe connects storage area, and a mouth of pipe connects transparent water pipe, and the remaining mouth of pipe is discharge outlet.
CN201410018706.0A 2014-01-15 2014-01-15 A kind of rhizosphere soil is separated and the plant roots bag Synthetical cultivation device collected Expired - Fee Related CN103749205B (en)

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CN104285719B (en) * 2014-09-12 2017-09-12 张彦军 A kind of desert, deserted mountain tree planting container
CN106998714A (en) * 2014-12-03 2017-08-01 皇家飞利浦有限公司 Humidity regulation
CN107941990B (en) * 2017-10-23 2020-01-10 中国环境科学研究院 Device and method for testing phosphorus absorption capacity of roots of aquatic plants based on DGT technology
CN108476795A (en) * 2018-05-23 2018-09-04 江西省食品发酵研究所 A kind of experiment Se-rich farm products planting unit convenient for classification plantation
CN111642277B (en) * 2020-07-08 2021-12-10 江西省农业科学院土壤肥料与资源环境研究所 Plant culture device and method for collecting rhizosphere soil
CN113702096B (en) * 2021-09-18 2023-12-08 河南工程学院 Rhizosphere box soil sample collection device and collection method
CN115005028B (en) * 2022-06-24 2023-12-15 临沂市农业科学院 Pepper rhizosphere secretion collecting device and method
CN115211301B (en) * 2022-07-28 2024-04-19 盐城工学院 Device for researching carbon and rhizosphere and use method

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CN202083567U (en) * 2011-04-13 2011-12-21 上海大学 Device for collecting secretion of plant root
CN102550324B (en) * 2012-02-14 2013-10-09 中国科学院植物研究所 Experimental device for researching partial root zone
KR101408995B1 (en) * 2012-03-12 2014-06-17 강성훈 vegetables cultivation vessel
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