CN114375695B - In-situ collecting device and method for hygrophyte root exudates - Google Patents
In-situ collecting device and method for hygrophyte root exudates Download PDFInfo
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- CN114375695B CN114375695B CN202111631773.6A CN202111631773A CN114375695B CN 114375695 B CN114375695 B CN 114375695B CN 202111631773 A CN202111631773 A CN 202111631773A CN 114375695 B CN114375695 B CN 114375695B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01D91/00—Methods for harvesting agricultural products
- A01D91/02—Products growing in the soil
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The invention discloses an in-situ collecting device and a collecting method for hygrophyte root exudates, wherein the collecting device comprises a collecting box with an opening at the top and a collecting cover matched with the collecting box, and the collecting cover is detachably and hermetically connected with the collecting box; the inside of the collecting box is filled with a root system culture medium for culturing plants; the collecting box is provided with a first channel for the plant root to pass through; the inner bottom surface of the collecting box is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box is higher than that of the inner bottom surface of the tail end of the collecting box; a sample injection needle tube and a sample outlet needle tube are hermetically arranged on the collecting cover; the collecting box and the collecting cover are respectively provided with the mounting groove and the tenon, so that the collecting cover can be opened or closed in a pulling manner, the root system and the culture medium can be conveniently put in or taken out, and the damage to the root system in the experimental process is reduced; and be provided with the sealing washer between mounting groove and tenon, make after the mounting groove is closed to collect the box and keep apart with external, it is sealed effectual, fine the entering of preventing outside solution is applicable to the wet environment of giving birth to.
Description
Technical Field
The invention relates to the technical field of plant, soil, agriculture and environmental science, in particular to an in-situ collecting device and a collecting method for hygrophyte root exudates.
Background
The root exudates play an important role in regulating and controlling the exchange of materials, energy and information among the rhizosphere microenvironment of the land ecosystem. The accurate acquisition of the information such as the variety, the content, the response to the environmental change and the like of the root exudates is a key link and a precondition for understanding the plant root system 8259, the ecological process of the soil interface and the information exchange. The method plays an extremely important role in revealing the functions of a soil ecosystem, guiding biological invasion prevention and control, ecological prevention and control of farmland weeds, establishing a comprehensive nutrient resource management system with coordinated crop high yield and environmental protection, and improving ecological agriculture management and popularization. The root exudates play an important role in regulating and controlling the exchange of materials, energy and information among the rhizosphere microenvironment of the land ecosystem. The accurate acquisition of the information such as the variety, the content, the response to the environmental change and the like of the root exudates is a key link and a precondition for understanding the plant root system 8259, the ecological process of the soil interface and the information exchange. The method has extremely important functions on revealing the functions of a soil ecosystem, guiding biological invasion prevention and control, ecological prevention and control of farmland weeds, establishing a comprehensive nutrient resource management system with coordinated crop high yield and environmental protection, improving ecological agriculture management and popularization.
Traditional root exudate normal position is collected and is adopted glass syringe as the root system and cultivateed the room. The in-situ root system is difficult to take out after being put in and is easy to damage; the culture medium (glass sand) is inconvenient to add and is easy to cause secondary damage to the root system. The sealing effect of the culture chamber is poor, the culture chamber is easily polluted by soil solution, and the culture chamber is not suitable for wetland environment such as swamp and wetland environment.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an in-situ collecting device for hygrophyte root exudates and a collecting method thereof, aiming at solving the problems that the in-situ root system is difficult to take out after being put in, the root system is easy to damage and the device is not suitable for a hygrophyte environment due to poor sealing effect of the prior collecting device for the plant root exudates.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: the in-situ collecting device for the hygrophyte root exudates comprises a collecting box, wherein the top of the collecting box is provided with an opening, a collecting cover is arranged on the opening, and the collecting cover is detachably and hermetically connected with the collecting box; the collecting cover and the collecting box are made of transparent materials; the inside of the collecting box is filled with a root system culture medium for culturing plants;
the head end of the collecting box is provided with a first channel for the plant root system to pass through; the inner bottom surface of the collecting box is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box is higher than that of the inner bottom surface of the tail end of the collecting box;
a sample injection needle tube used for injecting the culture solution into the collection box and a sample outlet needle tube used for sucking the root secretion and the culture solution are hermetically arranged on the collection cover;
the material of collecting the lid and collecting the box is transparent material, can choose transparent ya keli material for use, adopts 3D printing technique shaping, and transparent root system of being convenient for observe cultivates the condition, culture solution interpolation etc..
Furthermore, a gap with the same height as the thickness of the collecting cover is arranged on the side wall of the tail end of the collecting box; be provided with the mounting groove on the inner wall of collection box opening part, the lateral wall epirelief of collecting the lid is provided with the tenon that matches with the mounting groove, is provided with the sealing washer between mounting groove and the tenon, and after tenon and the mounting groove cooperation, the tail end of collecting the lid is located the breach and collects the upper surface parallel and level of lid and the upper surface of collecting the box.
Furthermore, a joint is convexly arranged on the side surface of the head end of the collecting cover, a joint groove matched with the joint is arranged on the top surface of the head end of the collecting box, the joint is arranged behind the joint groove, and the outer wall of the side surface of the head end of the collecting cover is contacted with the inner wall of the side surface of the head end of the collecting box; the cross section of the first channel is of a semicircular structure, the first channel is arranged on the bottom surface of the joint groove, and the top of the first channel is connected with the bottom surface of the joint groove.
Furthermore, a head partition plate and a tail partition plate are respectively arranged at the head end and the tail end of the collection box, the head partition plate and the inner side wall of the head end of the collection box enclose to form a head external solution isolation chamber, and the tail partition plate and the inner side wall of the tail end of the collection box enclose to form a tail external solution isolation chamber;
the top surface of the head partition plate is provided with a second channel for the plant root system to pass through, the cross section of the second channel is in a semicircular structure, and the top of the second channel is connected with the top surface of the head partition plate;
the top surfaces of the head partition plate and the tail partition plate are respectively provided with a sealing gasket which is used for contacting with the lower end surface of the collecting box.
Further, the interior bottom surface of collecting the box is sunken downwards and is provided with the collecting vat, and the collecting vat is the big triangular prism structure of head end little tail end, and the head end terminal surface of collecting the box is collected towards the head end of collecting the collecting vat and meets with the head baffle, and the tail end of collecting the box is collected towards the tail end of collecting the collecting vat and meets with the afterbody baffle.
Furthermore, the head end of the collecting cover is provided with a sample inlet hole, the tail end of the collecting cover is provided with a sample outlet hole, the sample inlet hole and the sample outlet hole are internally provided with a rubber plug, and the outer walls of the two rubber plugs are respectively contacted with the inner walls of the sample inlet hole and the sample outlet hole; the sample injection needle tube penetrates through the rubber plug in the sample injection hole, and the end part of the sample injection needle tube is flush with the lower surface of the rubber plug; the sample outlet needle tube penetrates through the rubber plug in the sample outlet hole, and the end part of the sample outlet needle tube is located at the lowest position of the collecting groove.
Further, a base with supporting legs is arranged at the bottom of the collecting box.
The invention also provides a collecting method of the in-situ collecting device for the hygrophyte root exudates, which is characterized by comprising the following steps:
step 1: excavating and cleaning out a complete plant root system, placing the plant root system in a collecting box, and placing a root system main rod of a plant in the collecting box by penetrating through a first channel and a second channel;
step 2: filling a culture medium into the collection box to cover the plant root system with the culture medium;
and step 3: closing the collecting cover to enable the collecting box to be in an isolated and sealed state relative to the outside;
and 4, step 4: a sample injection needle tube and a sample discharge needle tube are respectively inserted into the collecting cover, the end part of the sample injection needle tube is flush with the lower surface of the rubber plug, and the end part of the sample discharge needle tube is positioned at the lowest position of the collecting groove;
and 5: putting the collecting box and the collecting cover into the original sound environment of the plant root system, and covering black cloth on the collecting box and the collecting cover if the plant root system is a terrestrial plant root system; if the plant root is the aquatic plant root, the collecting box and the collecting cover are not covered with black cloth; injecting a preset amount of culture solution into the collection box by the sample injection needle tube, culturing the plant root system for a preset time, and then entering the step 6;
step 6: and (3) washing plant roots: extracting the culture solution from the sample outlet needle tube, injecting new culture solution into the sample inlet needle tube until the number of times is preset, and then entering the step 7;
and 7: culturing the plant root system for a preset time, extracting the plant root system secretion solution through a sample outlet needle tube, and analyzing to obtain the carbon concentration of the plant root system secretion solution;
and 8: opening the collecting cover to take out the plant root system, and performing live root scanning on the plant root system to obtain root system length, surface area data and dried root dry weight data;
and step 9: and 7, obtaining the root secretion amount of the plant root system generated in the preset time according to the extracted plant root secretion solution, the carbon concentration of the plant root secretion solution, the root length and surface area data in the step 8 and the dry weight data of the dried root.
C = K×M
wherein C is the amount of the root exudates, K is the carbon concentration of the plant root exudates solution, and M is the total volume of the extracted plant root exudates solution;
and (3) calculating the root secretion amount of the root system per hour in unit length, wherein the calculation formula is as follows:
X= (K×M)/(T×L) ；
wherein X is the root secretion amount of the root system per hour in unit length, T is preset time, and L is the length of the root system;
and (3) calculating the root secretion amount of the root system per hour on the unit surface area, wherein the calculation formula is as follows:
Y= (K×M)/(T×S)；
wherein Y is the root secretion amount of the root system per hour on unit surface area, and S is the surface area of the root system;
and (3) calculating the root secretion amount of the root system per hour by unit dry weight, wherein the calculation formula is as follows:
Z= (K×M)/(T×B)；
wherein Z is the root secretion amount of the root system per hour in unit dry weight, and B is the dry weight of the dried root system.
The beneficial effects of the invention are as follows: 1. in the scheme, the collecting box and the collecting cover are respectively provided with the mounting groove and the tenon, so that the collecting cover can be opened or closed in a pulling mode, the root system and the culture medium can be conveniently put in or taken out, and the damage to the root system in the experimental process is reduced; and be provided with the sealing washer between mounting groove and tenon, make after the mounting groove is closed collect box and keep apart with the external world, it is sealed effectual, fine prevent the entering of outside solution, be applicable to the wet environment of giving birth to.
2. Be provided with the external solution isolation room of head and the external solution isolation room of afterbody respectively at the both ends of collecting the box in this scheme, when outside solution got into and collects the box, at first get into the external solution isolation room of head or the external solution isolation room of afterbody, form first protective screen, prevent to collect the plant secretion solution in the box and be contaminated. After the external solution isolation chamber is filled, the sealing gasket forms a second barrier to prevent external solution from entering. The head external solution isolation chamber and the tail external solution isolation chamber can also be used as an index for judging whether the secretion is polluted or not. When the head external solution isolation chamber and the tail external solution isolation chamber are filled with the external solution, the secretion is polluted at great risk, and the solution in the collector can be abandoned and collected again.
3. In this scheme be provided with the collecting vat in collecting the box, the collecting vat is the big triangular prism structure of head end little tail end for plant roots secretion and culture solution are collected more conveniently, and are more thorough.
Drawings
Fig. 1 is a three-dimensional structure schematic diagram of a moist plant root exudate in-situ collection device.
Fig. 2 is a schematic diagram of the internal structure of an in-situ collecting device for the root exudates of a hygrophyte.
Wherein, 1, collecting box; 2. a collection cover; 3. a first channel; 4. a sample injection needle tube; 5. a sample outlet needle tube; 6. a notch; 7. mounting grooves; 8. a tenon; 9. a joint; 10. a joint groove; 11. a head partition plate; 12. a tail baffle plate; 13. a solution isolation chamber outside the head; 14. a tail external solution isolation chamber; 15. a second channel; 16. collecting tank; 17. a sample inlet hole; 18. a sample outlet; 19. a rubber plug; 20. a base; 21. a plant root system; 22. and (4) a culture medium.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate the understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and it will be apparent to those skilled in the art that various changes may be made without departing from the spirit and scope of the invention as defined and defined by the appended claims, and all changes that can be made by the invention using the inventive concept are intended to be protected.
As shown in fig. 1-2, an in-situ collecting device for the root exudates of hygrophytes is provided, which comprises a collecting box 1, wherein an opening is formed in the top of the collecting box 1, a collecting cover 2 is arranged on the opening, and the collecting cover 2 is detachably and hermetically connected with the collecting box 1; collect lid 2 and the material of collecting box 1 and be transparent material, can choose transparent acrylic material for use, adopt the 3D printing technique shaping, the transparent root system condition of cultivateing of being convenient for survey, culture solution interpolation etc..
The collecting box 1 is internally filled with a root system culture medium for culturing plants; the head end of the collecting box 1 is provided with a first channel 3 for the plant root system 21 to pass through; the inner bottom surface of the collecting box 1 is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box 1 is higher than that of the inner bottom surface of the tail end of the collecting box 1; the collection cover 2 is hermetically provided with a sample injection needle tube 4 for injecting culture solution into the collection box 1 and a sample outlet needle tube 5 for sucking root exudates and culture solution. Collect lid 2 and collect box 1 and can dismantle sealing connection, make things convenient for root system and culture medium to put into and take out, reduce the experimentation and harm the root system.
Preferably but not limitatively, the side wall of the tail end of the collecting box 1 is provided with a notch 6 with the height same as the thickness of the collecting cover 2; be provided with mounting groove 7 on the inner wall of collection box 1 opening part, the protruding tenon 8 that is provided with and matches with mounting groove 7 on the lateral wall of collecting lid 2, is provided with the sealing washer between mounting groove 7 and the tenon 8, and after tenon 8 and the 7 cooperation of mounting groove, the tail end of collecting lid 2 is located breach 6 and collects the upper surface parallel and level of lid 2 and the upper surface of collecting box 1. The collecting box 1 and the collecting cover 2 are respectively provided with the mounting groove 7 and the tenon 8, so that the collecting cover 2 can be opened or closed in a drawing way, the root system and the culture medium can be conveniently put in and taken out, and the damage to the root system in the experimental process is reduced; and be provided with the sealing washer between mounting groove 7 and tenon 8, make after mounting groove 7 is closed collect box 1 and keep apart with the external world, it is sealed effectual, fine prevent the entering of outside solution, be applicable to the wet environment of giving birth to.
Specifically, a joint 9 is convexly arranged on the side face of the head end of the collecting cover 2, a joint groove 10 matched with the joint 9 is arranged on the top face of the head end of the collecting box 1, the joint 9 is arranged behind the joint groove 10, and the outer wall of the side face of the head end of the collecting cover 2 is contacted with the inner wall of the side face of the head end of the collecting box 1; the cross section of the first channel 3 is in a semicircular structure, the first channel 3 is arranged on the bottom surface of the joint groove 10, and the top of the first channel 3 is connected with the bottom surface of the joint groove 10.
Furthermore, a head partition plate 11 and a tail partition plate 12 are respectively arranged at the head end and the tail end of the collecting box 1, the head partition plate 11 and the inner side wall of the head end of the collecting box 1 enclose to form a head external solution isolation chamber 13, and the tail partition plate 12 and the inner side wall of the tail end of the collecting box 1 enclose to form a tail external solution isolation chamber 14;
the top surface of the head partition plate 11 is provided with a second channel 15 for the plant root system 21 to pass through, the cross section of the second channel 15 is in a semicircular structure, and the top of the second channel 15 is connected with the top surface of the head partition plate 11;
the top surfaces of the head partition plate 11 and the tail partition plate 12 are respectively provided with a sealing gasket used for contacting with the lower end surface of the collecting box 1. The two ends of the collecting box are respectively provided with a head external solution isolation chamber 13 and a tail external solution isolation chamber 14, when external solution enters the collecting box 1, the external solution enters the head external solution isolation chamber 13 or the tail external solution isolation chamber 14 at first to form a first barrier, and the plant secretion solution in the collecting box 1 is prevented from being polluted. After the external solution isolation chamber is filled, the sealing gasket forms a second barrier to prevent external solution from entering. The cephalad ambient solution compartment 13 and caudal ambient solution compartment 14 may also be used as an indicator to determine whether the exudate is contaminated. When the head external solution isolation chamber 13 and the tail external solution isolation chamber 14 are filled with external solution, the secretion is polluted at a great risk, and the solution in the collector can be abandoned and collected again.
Collect and be provided with collecting vat 16 in the bottom surface undercut in box 1, collecting vat 16 is the big triangular prism structure of head end little tail end, and collecting vat 16's head end orientation is collected the head end terminal surface of box 1 and is met with head baffle 11, and collecting vat 16's tail end orientation is collected the tail end of box 1 and is met with afterbody baffle 12. The collecting tank 16 is a triangular prism structure with a small head end and a big tail end, so that the secretion of the plant root system and the culture solution can be collected more conveniently and thoroughly.
The head end of the collecting cover 2 is provided with a sample inlet hole 17, the tail end of the collecting cover is provided with a sample outlet hole 18, the sample inlet hole 17 and the sample outlet hole 18 are both internally provided with a rubber plug 19, and the outer walls of the two rubber plugs 19 are respectively contacted with the inner walls of the sample inlet hole 17 and the sample outlet hole 18; the sample injection needle tube 4 penetrates through the rubber stopper 19 in the sample injection hole 17, and the end part of the sample injection needle tube is flush with the lower surface of the rubber stopper 19, so that the root system is prevented from being injured by the bottom of the poplar needle tube; the sample outlet needle tube 5 penetrates through the rubber plug 19 in the sample outlet hole 18, and the end part of the sample outlet needle tube is located at the lowest position of the collecting groove 16, so that the plant root secretion and the culture solution are collected more conveniently and completely.
Further, a base 20 with legs is provided at the bottom of the collecting box 1. The base 20 is provided to facilitate the installation and fixation of the whole collecting device.
The invention also provides a collecting method of the in-situ collecting device for the hygrophyte root exudates, which is characterized by comprising the following steps:
step 1: digging and cleaning a complete plant root system 21, placing the plant root system 21 in the collecting box 1, and placing a root system main rod of the plant in the collecting box 1 by penetrating through the first channel 3 and the second channel 15;
step 2: filling a culture medium into the collection box to cover the plant root system 21 with the culture medium; the culture medium may be vermiculite.
And 3, step 3: closing the collection cover to enable the collection box 1 to be in an isolated and sealed state relative to the outside;
and 4, step 4: a sample injection needle tube 4 and a sample discharge needle tube 5 are respectively inserted into the collecting cover, the end part of the sample injection needle tube 4 is flush with the lower surface of the rubber stopper 19, and the end part of the sample discharge needle tube 5 is positioned at the lowest position of the collecting groove 16;
and 5: placing the collecting box 1 and the collecting cover 2 in a plant root system acoustic environment, and covering black cloth on the collecting box 1 and the collecting cover 2 if the plant root system is a terrestrial plant root system; if the plant root is the aquatic plant root, the collection box 1 and the collection cover 2 are not covered with black cloth; injecting a preset amount of culture solution into the collection box by the sample injection needle tube 4, culturing the plant root system 21 for a preset time, and then entering the step 6; the culture solution is a carbon-free mineral solution matched with the primary environment of the plant root system 21;
step 6: and (3) washing the plant root system 21: extracting the culture solution from the sample outlet needle tube 5, injecting new culture solution into the sample inlet needle tube 4 until the preset times, and then entering the step 7; extracting culture solution, adding new culture solution, washing for 3-5 times, and eliminating interference.
And 7: culturing the plant root system 21 for a preset time, extracting the plant root system secretion solution by the sample outlet needle tube 5, and analyzing to obtain the carbon concentration of the plant root system secretion solution;
and 8: opening the collecting cover to take out the plant root system 21, and performing live root scanning on the plant root system 21 to obtain the length and surface area data of the root system and the dry weight data of the dried root;
and step 9: and in the step 7, obtaining the root secretion amount of the plant root system 21 generated within the preset time according to the extracted plant root secretion solution, the carbon concentration of the plant root secretion solution, the root length and surface area data in the step 8 and the dry weight data of the dried root.
C = K×M
wherein C is the amount of the root exudates, K is the carbon concentration of the plant root exudates solution, and M is the total volume of the extracted plant root exudates solution;
and (3) calculating the root secretion amount of the root system per hour in unit length, wherein the calculation formula is as follows:
X= (K×M)/(T×L) ；
wherein X is the root secretion amount of the root system per hour in unit length, T is preset time, and L is the length of the root system;
and (3) calculating the root secretion amount of the root system per hour on the unit surface area, wherein the calculation formula is as follows:
Y= (K×M)/(T×S)；
wherein Y is the root secretion amount of the root system per hour on unit surface area, and S is the surface area of the root system;
and (3) calculating the root secretion amount of the root system per hour by unit dry weight, wherein the calculation formula is as follows:
Z= (K×M)/(T×B)；
wherein Z is the root secretion amount of the root system per hour in unit dry weight, and B is the dry weight of the dried root system.
Claims (8)
1. The in-situ collecting device for the root exudates of the hygrophytes is characterized by comprising a collecting box, wherein the top of the collecting box is provided with an opening, a collecting cover is arranged on the opening, and the collecting cover is detachably and hermetically connected with the collecting box; the collecting cover and the collecting box are made of transparent materials;
the inside of the collecting box is filled with a root system culture medium for culturing plants;
the head end of the collecting box is provided with a first channel for the plant root system to pass through; the inner bottom surface of the collecting box is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box is higher than that of the inner bottom surface of the tail end of the collecting box;
a sample injection needle tube used for injecting culture solution into the collection box and a sample outlet needle tube used for sucking root exudates and culture solution are hermetically arranged on the collection cover; a gap with the same height as the thickness of the collecting cover is arranged on the side wall of the tail end of the collecting box;
the inner wall of the opening of the collecting box is provided with a mounting groove, a tenon matched with the mounting groove is convexly arranged on the side wall of the collecting cover, a sealing ring is arranged between the mounting groove and the tenon, after the tenon is matched with the mounting groove, the tail end of the collecting cover is positioned in the notch, and the upper surface of the collecting cover is flush with the upper surface of the collecting box;
the head end and the tail end of the collecting box are respectively provided with a head partition plate and a tail partition plate, the head partition plate and the inner side wall of the head end of the collecting box enclose to form a head external solution isolation chamber, and the tail partition plate and the inner side wall of the tail end of the collecting box enclose to form a tail external solution isolation chamber;
the top surface of the head partition plate is provided with a second channel for the plant root system to pass through, the cross section of the second channel is in a semicircular structure, and the top of the second channel is connected with the top surface of the head partition plate;
the top surfaces of the head partition plate and the tail partition plate are respectively provided with a sealing gasket which is used for being in contact with the lower end surface of the collecting box.
2. The in-situ collecting device for the root exudates of hygrophytes according to claim 1, wherein a joint is convexly arranged on the side surface of the collecting cover head end, a joint groove matched with the joint is arranged on the top surface of the collecting box head end, and after the joint is arranged in the joint groove, the outer wall of the side surface of the collecting cover head end is contacted with the inner wall of the side surface of the collecting box head end; the cross section of the first channel is of a semicircular structure, the first channel is arranged on the bottom surface of the joint groove, and the top of the first channel is connected with the bottom surface of the joint groove.
3. The in-situ collecting device for the secretion of the roots of hygrophytes as claimed in claim 2, wherein a collecting tank is arranged on the inner bottom surface of the collecting box in a downward concave manner, the collecting tank is of a triangular prism structure with a small head end and a large tail end, the head end of the collecting tank faces the head end face of the collecting box and is connected with the head partition plate, and the tail end of the collecting tank faces the tail end of the collecting box and is connected with the tail partition plate.
4. The in-situ collecting device for the root exudates of hygrophytes according to claim 3, wherein the head end of the collecting cover is provided with a sample inlet hole, the tail end of the collecting cover is provided with a sample outlet hole, the sample inlet hole and the sample outlet hole are both internally provided with a rubber plug, the outer walls of the two rubber plugs are respectively contacted with the inner walls of the sample inlet hole and the sample outlet hole; the sample injection needle tube penetrates through the rubber plug in the sample injection hole, and the end part of the sample injection needle tube is flush with the lower surface of the rubber plug; the sample outlet needle tube penetrates through the rubber plug in the sample outlet hole, and the end part of the sample outlet needle tube is located at the lowest position of the collecting groove.
5. The in-situ collecting device for the hygrophyte root exudates as claimed in any one of claims 1 to 4, wherein a base with supporting legs is arranged at the bottom of the collecting box.
6. A method for collecting in-situ collecting device of wet plant root exudates according to claim 5, comprising:
step 1: digging and cleaning a complete plant root system, placing the plant root system in a collecting box, and placing a root system main rod of a plant in the collecting box by penetrating through a first channel and a second channel;
step 2: filling a culture medium into the collection box to cover the root system of the plant;
and 3, step 3: closing the collecting cover to enable the collecting box to be in an isolated and sealed state relative to the outside;
and 4, step 4: a sample injection needle tube and a sample discharge needle tube are respectively inserted into the collecting cover, the end part of the sample injection needle tube is flush with the lower surface of the rubber plug, and the end part of the sample discharge needle tube is positioned at the lowest position of the collecting groove;
and 5: putting the collecting box and the collecting cover into the original sound environment of the plant root system, and covering black cloth on the collecting box and the collecting cover if the plant root system is a terrestrial plant root system; if the plant root is the aquatic plant root, the collecting box and the collecting cover are not covered with black cloth; injecting a preset amount of culture solution into the collection box by the sample injection needle tube, culturing the plant root system for a preset time, and then entering the step 6;
and 6: and (3) washing the plant root system: extracting the culture solution from the sample outlet needle tube, injecting new culture solution into the sample inlet needle tube until the times are preset, and then entering the step 7;
and 7: culturing the plant root system for a preset time, extracting the plant root system secretion solution through a sample outlet needle tube, and analyzing to obtain the carbon concentration of the plant root system secretion solution;
and 8: opening the collecting cover to take out the plant root system, and performing live root scanning on the plant root system to obtain root system length, surface area data and dried root dry weight data;
and step 9: and 7, obtaining the root secretion amount of the plant root system generated in the preset time according to the extracted plant root secretion solution, the carbon concentration of the plant root secretion solution, the root length and surface area data in the step 8 and the dry weight data of the dried root.
7. The method for collecting the in-situ wet plant root exudate (SOS) collecting device of claim 6, wherein the step 6 further comprises the following steps: the frequency of washing the plant root system is three times; injecting a fixed volume of culture solution Ma, extracting a volume N1 of root exudate solution for the first time, adding the fixed volume of culture solution Ma after extraction, extracting a volume N1 of the root exudate solution for the second time, adding the fixed volume of culture solution Ma, and extracting a volume N1 of the root exudate solution for the third time;
the step 7 further comprises: the times of extracting the plant root secretion solution are three times, and the volume of the first extracted plant root secretion solution is M 1 The culture solution of the volume of injecting Ma into in the collection box after the extraction, the volume of extraction plant roots secretion solution for the second time is M2, and the culture solution of the volume of injecting Ma into in the collection box after the extraction, the volume of extraction plant roots secretion solution for the third time is M3, and the culture solution of the volume of injecting Ma into in the collection box after the extraction, the total volume of extraction plant roots secretion solution is M, M = M1+ M2+ M3.
8. The method for collecting the in-situ collecting device of the wet plant root exudates as claimed in claim 7, wherein the step 9 further comprises: calculating the root secretion amount of the plant root system generated in the preset time, wherein the calculation formula is as follows:
C = K×M
wherein C is the root exudate amount, K is the carbon concentration of the plant root exudate solution, and M is the total volume of the extracted plant root exudate solution;
and (3) calculating the root secretion amount of the root system per hour in unit length, wherein the calculation formula is as follows:
X= (K×M)/(T×L) ；
wherein X is the root secretion amount of the root system per hour in unit length, T is preset time, and L is the length of the root system;
and (3) calculating the root secretion amount of the root system per hour on the unit surface area, wherein the calculation formula is as follows:
Y= (K×M)/(T×S)；
wherein Y is the root secretion amount per hour of the root system with unit surface area, and S is the surface area of the root system;
and (3) calculating the root secretion amount of the root system per hour by unit dry weight, wherein the calculation formula is as follows:
Z= (K×M)/(T×B)；
wherein Z is the root secretion amount of the root system per hour in unit dry weight, and B is the dry weight of the dried root system.
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