CN207964788U - A kind of root exudates collection device suitable for spacing sleeve cropping system - Google Patents

A kind of root exudates collection device suitable for spacing sleeve cropping system Download PDF

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Publication number
CN207964788U
CN207964788U CN201820483202.XU CN201820483202U CN207964788U CN 207964788 U CN207964788 U CN 207964788U CN 201820483202 U CN201820483202 U CN 201820483202U CN 207964788 U CN207964788 U CN 207964788U
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CN
China
Prior art keywords
root
root exudates
collection device
donor plant
spacing sleeve
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Expired - Fee Related
Application number
CN201820483202.XU
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Chinese (zh)
Inventor
杨雪丽
雍太文
刘凡
吴吉英子
陈平
刘旭东
汪锦
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Sichuan Agricultural University
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Sichuan Agricultural University
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Priority to CN201820483202.XU priority Critical patent/CN207964788U/en
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Publication of CN207964788U publication Critical patent/CN207964788U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model discloses a kind of root exudates collection devices suitable for spacing sleeve cropping system, the root exudates collection device cultivates one recipient plant cultivating chamber of design in box at one, two donor plant cultivating chambers and a donor plant root growth room, donor plant root growth room is connected with switch filter device and root exudates conduit, it can easily realize the enrichment of root exudates, it collects and pours, it can collect repeatedly, reduce waste, overall structure is simple, it is easy to make, it is rationally distributed, field in-situ observation is good, with universality, it can be used as nonoculture, between make or set operation mode, it is convenient adjustable, portion forms index and physical signs can be observed simultaneously, with functional diversity.

Description

A kind of root exudates collection device suitable for spacing sleeve cropping system
Technical field
The utility model belongs to agricultural technology field, specifically, be related to it is a kind of suitable between or set operation mode and farmland The collection device of root exudates under crop sand culture mode.
Background technology
Corn soybean intercropping and interplanting pattern and its supporting technology greatly improve the yield of soya corn.But by big Area investigation is found, makees or cover the problem of operation mode restricts its yield and benefit there is also some, especially root between corn soybean System's growth and root system are secreted and are led to the problem of.The root exudates collection device of currently the majority, both for nonoculture mode, Between lacking make or interplanting under the conditions of root exudates collection device.
From the point of view of laboratory test operational circumstances, the root exudates collection device of nonoculture is made or is covered between can not meeting The research of corn soybean root exudates under operation mode.The reason is that making or covering the root exudates under operation mode between collecting, plant Training condition needs to meet simultaneously with collection process, and the degree-of-difficulty factor of design is opposite to be increased.
Utility model content
The purpose of this utility model is that solve the above-mentioned problems and provides a kind of suitable for spacing sleeve cropping system Root exudates collection device.
The utility model is achieved through the following technical solutions above-mentioned purpose:
A kind of root exudates collection device suitable for spacing sleeve cropping system, including box is cultivated, it is set in the cultivation box It is equipped with donor plant root growth room and the upper chamber above donor plant root growth room, the upper chamber is logical It crosses two pieces of partition boards and is divided into two donor plant cultivating chambers and the recipient plant cultivation between two donor plant cultivating chambers Train room;
It is separated by agar plate between the donor plant cultivating chamber and donor plant root growth room;
It is detachable connection structure between the partition board and the inner wall of the upper chamber;
Donor plant root growth room is provided with root exudates outlet, and the root exudates outlet is successively It is provided with switch valve I, filter I and three-way pipe, the first end of the three-way pipe is connect with the outlet of the filter I, and second End is connected with root exudates conduit, and third end is as the air pump interface for being connect with air pump.
As a kind of technical solution of this patent selection, the partition board tilts installation, the recipient plant cultivating chamber and institute The trapezium structure that donor plant root growth room is handstand is stated, two donor plant cultivating chambers are parallelogram knot Structure.
As a kind of technical solution of this patent selection, the inner wall inclination of the upper chamber offers slot, the partition board It is plugged in the slot.
As a kind of technical solution of this patent selection, the partition board is nylon web plate or plastic film plates.
As a kind of technical solution of this patent selection, the agar plate includes being fixedly connected on inside the cultivation box Hollow out orifice plate and the agar layer being poured in the hollow out orifice plate.
As a kind of technical solution of this patent selection, the root exudates conduit is by two sections of grass tubes and is connected to Rubber catheter composition between two sections of grass tubes, wherein one section of grass tube is connect with the three-way pipe.
As a kind of technical solution of this patent selection, the grass tube length being connect with the three-way pipe is 50cm, institute It is 30cm to state rubber catheter length, and another section of grass tube length is 10cm.
As a kind of technical solution of this patent selection, the front and rear sides wall of the recipient plant cultivating chamber is transparent Glass wall.
As a kind of technical solution of this patent selection, the recipient plant cultivating chamber is provided with waste liquid outlet, described Waste liquid outlet is disposed with switch valve II and filter II.
Compared with prior art, the utility model has the advantages that:
The root exudates collection device is cultivated at one designs a recipient plant cultivating chamber, two donor plants in box Cultivating chamber and a donor plant root growth room, donor plant root growth room are connected with switch filter device and root system secretion Object conduit can easily realize the enrichment, collection and pouring of root exudates, can collect repeatedly, reduce waste, whole Simple in structure, easy to make, rationally distributed, field in-situ observation is good, has universality, can be used as nonoculture, work or set Operation mode, it is convenient adjustable, portion forms index and physical signs can be observed simultaneously, and there is functional diversity.
Description of the drawings
Fig. 1 is the main broken section of the root exudates collection device described in the utility model suitable for spacing sleeve cropping system Structural schematic diagram;
Fig. 2 is that the plan structure of the root exudates collection device described in the utility model suitable for spacing sleeve cropping system is shown It is intended to;
In above-mentioned attached drawing, the corresponding component names of reference numeral are as follows:
1- cultivates box, 2- donor plant cultivating chambers, 3- recipient plant cultivating chambers, 4- donor plant cultivating chambers, the plant of 5- donors Object root growth room, 6- slots, 7- partition boards, 8- agar plates, 9- switch valves II, 10- filters II, 11- switch valves I, 12- filterings Device I, 13- three-way pipes, 14- root exudates conduits, 141- grass tubes, 142- rubber catheters, 143- grass tubes.
Specific implementation mode
The utility model is described in further detail below in conjunction with the accompanying drawings:
In conjunction with shown in Fig. 1 and Fig. 2, the utility model includes cultivating box 1, cultivates and is provided with the life of donor plant root system in box 1 Long room 5 and the upper chamber above donor plant root growth room 5, upper chamber are divided into two donors by two pieces of partition boards 7 Plant culture room 2,4 and the recipient plant cultivating chamber 3 between two donor plant cultivating chambers;
It is separated by agar plate 8 between donor plant cultivating chamber and donor plant root growth room 5, the design of agar plate 8 It is in order to which for root growth, donor plant root growth room 5 can be entered to avoid soil particle, the soil liquid, pollution donor is planted Trapping medium and root exudates in object root growth room 5, in growth course, donor plant root system may pass through agar plate 8 And it extends in donor plant root growth room 5;
It is detachable connection structure between partition board 7 and the inner wall of upper chamber, the detachable design of partition board 7 is for not Consider with planting patterns, the partition board 7 selected by different planting patterns may be different;
Donor plant root growth room 5 is provided with root exudates outlet, and root exudates outlet is disposed with The first end of switch valve I 11, filter I 12 and three-way pipe 13, three-way pipe 13 is connect with the outlet of filter I 12, and second end connects It is connected to root exudates conduit 14, third end is equipped with XAD- as the air pump interface for being connect with air pump, I 12 inside of filter 4 absorption resins, weight 60g can adsorb the compound that 100 plants of plants (weight of root system is less than 1g plants) secrete for 24 hours.
As a kind of technical solution of this patent selection, partition board 7 tilts installation, recipient plant cultivating chamber 3 and donor plant Root growth room 5 is the trapezium structure of handstand, and two donor plant cultivating chambers are parallelogram sturcutre, the structure design The root of plant may make to collect growth in.
As a kind of technical solution of this patent selection, the inner wall inclination of upper chamber offers slot 6, and partition board 7 is plugged on In slot 6, which is a kind of simple Demountable, and dismounting is very convenient.
As a kind of technical solution of this patent selection, partition board 7 is nylon web plate or plastic film plates, according to floristic Suitably selected.
As a kind of technical solution of this patent selection, agar plate 8 includes being fixedly connected on the hollow hole cultivated inside box 1 Plate and the agar layer being poured in hollow out orifice plate, the perfusion of agar is more convenient, in the Root-development of donor plant, from fine jade Lipid layer is extended through to donor plant root growth room 5.
As a kind of technical solution of this patent selection, root exudates conduit 14 is by two sections of grass tubes 141,143 and The rubber catheter 142 being connected between two sections of grass tubes forms, wherein one section of grass tube 143 is connect with three-way pipe 13, rubber The action site of the conveniently adjusted root exudates of design of glue conduit 142;
Wherein, the grass tube length being connect with three-way pipe 13 is 50cm, and 142 length of rubber catheter is 30cm, Ling Yiduan Grass tube length is 10cm.
As a kind of technical solution of this patent selection, the front and rear sides wall of recipient plant cultivating chamber 3 is transparent glass Glass wall cultivates box 1 in addition to the glass wall of front and back side, and other parts are made of the stainless steel plate of 2mm thickness, transparent glass wall Design, convenient for the inner case of observation recipient plant cultivating chamber 3.
As a kind of technical solution of this patent selection, recipient plant cultivating chamber 3 is provided with waste liquid outlet, waste liquid discharge Mouth is disposed with switch valve II 9 and filter II 10, the waste liquid which is used to filter, be discharged in recipient plant cultivating chamber 3, Resin is adsorbed in II 10 inside of filter equipped with XAD-4, and weight 60g can adsorb 100 plants of plants (weight of root system is less than 1g plants) The compound secreted for 24 hours.
The operation principle of the utility model is as follows:
The utility model root exudates collection device can be used as nonoculture, work or set operation mode.It can directly interplanting see Examine vegetation growth state;Or soya corn is separated into plantation, and collect donor secretions from plant roots and made using pouring mode For recipient plant, its influence to recipient plant is observed.
The external blower pump of air pump interface, power 120W, air blowing pump operation time, generally to work per hour 10min is advisable, but is stopped using surveying recipient plant root exudates the last week.In plantation, plant culture contains sand in room Vermiculite, nutrient solution needed for training, plant seedlings can be transplanted into cultivating chamber, carry out cultivation culture.
The mode mode of action is poured in corn-soybean interplanting
Maize planting in recipient plant cultivating chamber 3, the donor plant cultivating chamber of both sides is interior to plant soybean, is sand culture mould Formula culture, partition board 7 use plastic film plates.Tri-leaf period is grown in soybean, starts to collect soybean root system secretion, turns on the switch valve I 11, soybean root system passes through agar plate 8 under the action of gravity and inclined plate pressure and is given birth to donor plant root growth room 5 It is long, soybean is poured with distilled water, so that root exudates is entered filter I 12 together therewith, is enriched with.By the root system of enrichment Secretion solution is pumped into root exudates conduit 14 by blower pump, is flowed out from top glass tube, moves root exudates conduit 14, at recipient plant, are poured.Before collecting maize root system secretion one week, valve I 11 is turned off the switch, stops collecting big Beans root exudates, blower pump are stopped.Space is saved in such design, is with conjunction two is poured by the collection of root exudates One, it is suitable for set operation mode, and the at the same time morphological index of observable maize root system, turns on the switch valve II 9, it can be in filter Purifying corn root exudates is collected in II 10, measures the ingredient of maize root system secretion under set operation mode.
Corn-soybean interplanting, the direct interplanting mode of action
Plant soybean in recipient plant cultivating chamber 3, maize planting in the donor plant cultivating chamber of both sides, is sand culture mould Formula culture, partition board 7 use 30 μm of nylon wires.Tri-leaf period is grown in corn, starts to collect maize root system secretion, turns on the switch valve I 11, maize root system is directly grown, and pours corn with distilled water, root exudates is made to enter filter I 12 together therewith, carry out Enrichment.Root exudates solution by enrichment is pumped into root exudates conduit 14, root exudates conduit 14 by blower pump Access wide-mouth bottle.Space is saved in such design, and the filtering of root exudates is combined into one with collection, is suitable for set operation mode, And the at the same time morphological index of observable maize root system.
Corn nonoculture measures Morphological And Physiological
Maize seed is directly entered into donor plant cultivating chamber, observes root growth, root exudates is collected and is recorded.
According to the above embodiment, the utility model can be realized well.It is worth noting that being set based on above structure Under the premise of meter, to solve same technical problem, even if some made on the utility model without substantive change or Polishing, the essence of used technical solution is still as the utility model, therefore it should also be as the protection in the utility model In range.

Claims (9)

1. a kind of root exudates collection device suitable for spacing sleeve cropping system, it is characterised in that:Including cultivating box, the training It plants in box and is provided with donor plant root growth room and the upper chamber above donor plant root growth room, it is described Upper chamber by two pieces of partition boards be divided into two donor plant cultivating chambers and between two donor plant cultivating chambers by Body plant culture room;
It is separated by agar plate between the donor plant cultivating chamber and donor plant root growth room;
It is detachable connection structure between the partition board and the inner wall of the upper chamber;
Donor plant root growth room is provided with root exudates outlet, and the root exudates outlet is set gradually There are switch valve I, filter I and three-way pipe, the first end of the three-way pipe to be connect with the outlet of the filter I, second end connects It is connected to root exudates conduit, third end is as the air pump interface for being connect with air pump.
2. being used for the root exudates collection device of spacing sleeve cropping system according to claim 1, it is characterised in that:It is described every Plate tilts installation, and the recipient plant cultivating chamber and donor plant root growth room are the trapezium structure of handstand, two The donor plant cultivating chamber is parallelogram sturcutre.
3. being used for the root exudates collection device of spacing sleeve cropping system according to claim 2, it is characterised in that:On described The inner wall inclination of floor room offers slot, and the partition board is plugged in the slot.
4. according to the root exudates collection device for being used for spacing sleeve cropping system described in claim 1,2 or 3, it is characterised in that: The partition board is nylon web plate or plastic film plates.
5. being used for the root exudates collection device of spacing sleeve cropping system according to claim 1, it is characterised in that:The fine jade Fat plate includes the hollow out orifice plate being fixedly connected on inside the cultivation box and the agar layer being poured in the hollow out orifice plate.
6. being used for the root exudates collection device of spacing sleeve cropping system according to claim 1, it is characterised in that:Described It is that secretion conduit is made of two sections of grass tubes and the rubber catheter being connected between two sections of grass tubes, wherein one section Grass tube is connect with the three-way pipe.
7. being used for the root exudates collection device of spacing sleeve cropping system according to claim 6, it is characterised in that:With it is described The grass tube length of three-way pipe connection is 50cm, and the rubber catheter length is 30cm, and another section of grass tube length is 10cm。
8. being used for the root exudates collection device of spacing sleeve cropping system according to claim 1, it is characterised in that:It is described by The front and rear sides wall of body plant culture room is transparent glass wall.
9. according to claim 1 or the 8 root exudates collection devices for being used for spacing sleeve cropping system, it is characterised in that:Institute It states recipient plant cultivating chamber and is provided with waste liquid outlet, the waste liquid outlet is disposed with switch valve II and filter II.
CN201820483202.XU 2018-04-04 2018-04-04 A kind of root exudates collection device suitable for spacing sleeve cropping system Expired - Fee Related CN207964788U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820483202.XU CN207964788U (en) 2018-04-04 2018-04-04 A kind of root exudates collection device suitable for spacing sleeve cropping system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820483202.XU CN207964788U (en) 2018-04-04 2018-04-04 A kind of root exudates collection device suitable for spacing sleeve cropping system

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717213A (en) * 2019-01-28 2019-05-07 广西大学 A kind of disease-resistant tomato stock root exudates collection liquid and its suppressing method to ralstonia solanacearum
CN114375695A (en) * 2021-12-28 2022-04-22 四川农业大学 In-situ collecting device and method for hygrophyte root exudates

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717213A (en) * 2019-01-28 2019-05-07 广西大学 A kind of disease-resistant tomato stock root exudates collection liquid and its suppressing method to ralstonia solanacearum
CN114375695A (en) * 2021-12-28 2022-04-22 四川农业大学 In-situ collecting device and method for hygrophyte root exudates

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Granted publication date: 20181012

Termination date: 20200404