CN114375695A - In-situ collecting device and method for hygrophyte root exudates - Google Patents
In-situ collecting device and method for hygrophyte root exudates Download PDFInfo
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- CN114375695A CN114375695A CN202111631773.6A CN202111631773A CN114375695A CN 114375695 A CN114375695 A CN 114375695A CN 202111631773 A CN202111631773 A CN 202111631773A CN 114375695 A CN114375695 A CN 114375695A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01D91/00—Methods for harvesting agricultural products
- A01D91/02—Products growing in the soil
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
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- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses an in-situ collecting device and a collecting method for hygrophyte root exudates, wherein the collecting device comprises a collecting box with an opening at the top and a collecting cover matched with the collecting box, and the collecting cover is detachably and hermetically connected with the collecting box; the inside of the collecting box is filled with a root system culture medium for culturing plants; the collecting box is provided with a first channel for the plant root to pass through; the inner bottom surface of the collecting box is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box is higher than that of the inner bottom surface of the tail end of the collecting box; a sample injection needle tube and a sample outlet needle tube are hermetically arranged on the collecting cover; the collecting box and the collecting cover are respectively provided with the mounting groove and the tenon, so that the collecting cover can be opened or closed in a pulling manner, the root system and the culture medium can be conveniently put in or taken out, and the damage to the root system in the experimental process is reduced; and be provided with the sealing washer between mounting groove and tenon, make after the mounting groove is closed collect box and keep apart with the external world, it is sealed effectual, fine prevent the entering of outside solution, be applicable to the wet environment of giving birth to.
Description
Technical Field
The invention relates to the technical field of plant, soil, agriculture and environmental science, in particular to an in-situ collecting device and a collecting method for hygrophyte root exudates.
Background
The root exudates play an important role in regulating and controlling the exchange of materials, energy and information among the rhizosphere microenvironment of the land ecosystem. The accurate acquisition of the information such as the variety and the content of the root exudates and the response of the root exudates to the environmental change is a key link and a precondition for understanding the ecological process of the plant root system-soil interface and information exchange. The method has extremely important functions on revealing the functions of a soil ecosystem, guiding biological invasion prevention and control, ecological prevention and control of farmland weeds, establishing a comprehensive nutrient resource management system with coordinated crop high yield and environmental protection, improving ecological agriculture management and popularization. The root exudates play an important role in regulating and controlling the exchange of materials, energy and information among the rhizosphere microenvironment of the land ecosystem. The accurate acquisition of the information such as the variety and the content of the root exudates and the response of the root exudates to the environmental change is a key link and a precondition for understanding the ecological process of the plant root system-soil interface and information exchange. The method has extremely important functions on revealing the functions of a soil ecosystem, guiding biological invasion prevention and control, ecological prevention and control of farmland weeds, establishing a comprehensive nutrient resource management system with coordinated crop high yield and environmental protection, improving ecological agriculture management and popularization.
Traditional root exudate normal position is collected and is adopted glass syringe as the root system and cultivateed the room. The in-situ root system is difficult to take out after being put in and is easy to damage; the culture medium (glass sand) is inconvenient to add and is easy to cause secondary damage to the root system. The sealing effect of the culture chamber is poor, the culture chamber is easily polluted by soil solution, and the culture chamber is not suitable for wetland environment such as swamp and wetland environment.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an in-situ collecting device for hygrophyte root exudates and a collecting method thereof, aiming at solving the problems that the existing device for collecting the hygrophyte root exudates is difficult to take out after the in-situ root system is put in and easy to damage the root system and is not suitable for the hygrophyte environment due to poor sealing effect.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: the in-situ collecting device for the root exudates of the hygrophytes comprises a collecting box, wherein an opening is formed in the top of the collecting box, a collecting cover is arranged on the opening, and the collecting cover is detachably and hermetically connected with the collecting box; the collecting cover and the collecting box are made of transparent materials; the inside of the collecting box is filled with a root system culture medium for culturing plants;
the head end of the collecting box is provided with a first channel for the plant root system to pass through; the inner bottom surface of the collecting box is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box is higher than that of the inner bottom surface of the tail end of the collecting box;
a sample injection needle tube used for injecting the culture solution into the collection box and a sample outlet needle tube used for sucking the root secretion and the culture solution are hermetically arranged on the collection cover;
the material of collecting the lid and collecting the box is transparent material, can choose transparent ya keli material for use, adopts 3D printing technique shaping, and transparent root system of being convenient for observe cultivates the condition, culture solution interpolation etc..
Furthermore, a gap with the same height as the thickness of the collecting cover is arranged on the side wall of the tail end of the collecting box; be provided with the mounting groove on the inner wall of collection box opening part, the lateral wall epirelief of collecting the lid is provided with the tenon that matches with the mounting groove, is provided with the sealing washer between mounting groove and the tenon, and after tenon and the mounting groove cooperation, the tail end of collecting the lid is located the breach and collects the upper surface parallel and level of lid and the upper surface of collecting the box.
Furthermore, a joint is convexly arranged on the side surface of the collecting cover head end, a joint groove matched with the joint is arranged on the top surface of the collecting box head end, the joint is arranged behind the joint groove, and the outer wall of the side surface of the collecting cover head end is contacted with the inner wall of the side surface of the collecting box head end; the cross section of the first channel is of a semicircular structure, the first channel is arranged on the bottom surface of the joint groove, and the top of the first channel is connected with the bottom surface of the joint groove.
Furthermore, a head partition plate and a tail partition plate are respectively arranged at the head end and the tail end of the collecting box, the head partition plate and the inner side wall of the head end of the collecting box enclose to form a head external solution isolation chamber, and the tail partition plate and the inner side wall of the tail end of the collecting box enclose to form a tail external solution isolation chamber;
the top surface of the head partition plate is provided with a second channel for the plant root system to pass through, the cross section of the second channel is in a semicircular structure, and the top of the second channel is connected with the top surface of the head partition plate;
the top surfaces of the head partition plate and the tail partition plate are respectively provided with a sealing gasket which is used for contacting with the lower end surface of the collecting box.
Further, the interior bottom surface of collecting the box is sunken downwards and is provided with the collecting vat, and the collecting vat is the big triangular prism structure of head end little tail end, and the head end terminal surface of collecting the box is collected towards the head end of collecting the collecting vat and meets with the head baffle, and the tail end of collecting the box is collected towards the tail end of collecting the collecting vat and meets with the afterbody baffle.
Furthermore, the head end of the collecting cover is provided with a sample inlet hole, the tail end of the collecting cover is provided with a sample outlet hole, the sample inlet hole and the sample outlet hole are internally provided with a rubber plug, and the outer walls of the two rubber plugs are respectively contacted with the inner walls of the sample inlet hole and the sample outlet hole; the sample injection needle tube penetrates through the rubber plug in the sample injection hole, and the end part of the sample injection needle tube is flush with the lower surface of the rubber plug; the sample outlet needle tube penetrates through the rubber plug in the sample outlet hole, and the end part of the sample outlet needle tube is located at the lowest position of the collecting groove.
Further, a base with supporting legs is arranged at the bottom of the collecting box.
The invention also provides a collecting method of the in-situ collecting device for the hygrophyte root exudates, which is characterized by comprising the following steps:
step 1: excavating and cleaning out a complete plant root system, placing the plant root system in a collecting box, and placing a root system main rod of the plant outside the collecting box through a first channel and a second channel;
step 2: filling a culture medium into the mounting box, and enabling the culture medium to cover the plant root system;
and step 3: closing the mounting cover to enable the collecting box to be in an isolated and sealed state relative to the outside;
and 4, step 4: a sample injection needle tube and a sample discharge needle tube are respectively inserted into the mounting cover, the end part of the sample injection needle tube is flush with the lower surface of the rubber plug, and the end part of the sample discharge needle tube is positioned at the lowest position of the collecting groove;
and 5: putting the collecting box and the collecting cover into the original sound environment of the plant root system, and covering black cloth on the collecting box and the collecting cover if the plant root system is a terrestrial plant root system; if the plant root is the aquatic plant root, the collecting box and the collecting cover are not covered with black cloth; injecting a preset amount of culture solution into the installation box by the sample injection needle tube, culturing the plant root system for a preset time, and then entering the step 6;
step 6: and (3) washing the plant root system: extracting the culture solution from the sample outlet needle tube, injecting new culture solution into the sample inlet needle tube until the times are preset, and then entering the step 7;
and 7: culturing the plant root system for a preset time, extracting the plant root system secretion solution through a sample outlet needle tube, and analyzing to obtain the carbon concentration of the plant root system secretion solution;
and 8: opening the mounting cover to take out the plant root system, and performing live root scanning on the plant root system to obtain root system length, surface area data and dried root dry weight data;
and step 9: and 7, obtaining the root secretion amount of the plant root system generated in the preset time according to the extracted plant root secretion solution, the carbon concentration of the plant root secretion solution, the root length and surface area data in the step 8 and the dry weight data of the dried root.
Step 6 further comprises: the frequency of washing the plant root system is three times; inject into the fixed Ma of culture solution volume, extract root system secretion solution volume N1 for the first time, add fixed volume culture solution Ma after the extraction, extract root system secretion solution volume N1 for the second time, adding fixed volume culture solution Ma, extract root system secretion solution volume N1 for the third time, ensure that plant roots washes more clean thoroughly, avoid influencing in the follow-up step, acquire the precision of plant roots secretion solution volume.
Step 9 further comprises: calculating the root secretion amount of the plant root system generated in the preset time, wherein the calculation formula is as follows:
C＝K×M
wherein C is the root exudate amount, K is the carbon concentration of the plant root exudate solution, and M is the total volume of the extracted plant root exudate solution;
and (3) calculating the root secretion amount of the root system per hour in unit length, wherein the calculation formula is as follows:
X＝(K×M)/(T×L)；
wherein X is the root secretion amount of the root system per hour in unit length, T is preset time, and L is the length of the root system;
and (3) calculating the root secretion amount of the root system per hour on the unit surface area, wherein the calculation formula is as follows:
Y＝(K×M)/(T×S)；
wherein Y is the root secretion amount of the root system per hour on unit surface area, and S is the surface area of the root system;
and (3) calculating the root secretion amount of the root system per hour by unit dry weight, wherein the calculation formula is as follows:
Z＝(K×M)/(T×B)；
wherein Z is the root secretion amount of the root system per hour in unit dry weight, and B is the dry weight of the dried root system.
The invention has the beneficial effects that: 1. in the scheme, the collecting box and the collecting cover are respectively provided with the mounting groove and the tenon, so that the collecting cover can be opened or closed in a pulling mode, the root system and the culture medium can be conveniently put in or taken out, and the damage to the root system in the experimental process is reduced; and be provided with the sealing washer between mounting groove and tenon, make after the mounting groove is closed collect box and keep apart with the external world, it is sealed effectual, fine prevent the entering of outside solution, be applicable to the wet environment of giving birth to.
2. Be provided with the external solution isolation chamber of head and the external solution isolation chamber of afterbody respectively at the both ends of mounting box in this scheme, when outside solution got into and collects the box, at first get into the external solution isolation chamber of head or the external solution isolation chamber of afterbody, form first protective screen, prevent to collect the plant secretion solution in the box and be polluted. After the external solution isolation chamber is filled, the sealing gasket forms a second barrier to prevent external solution from entering. The head external solution isolation chamber and the tail external solution isolation chamber can also be used as an index for judging whether the secretion is polluted or not. When the head external solution isolation chamber and the tail external solution isolation chamber are filled with the external solution, the secretion is polluted at great risk, and the solution in the collector can be abandoned and collected again.
3. Be provided with the collecting vat in the mounting box in this scheme, the collecting vat is the big triangular prism structure of head end little tail end for plant roots secretion and culture solution are collected more conveniently, and are more thorough.
Drawings
Fig. 1 is a three-dimensional structure schematic diagram of a moist plant root exudate in-situ collection device.
Fig. 2 is a schematic diagram of the internal structure of an in-situ collecting device for the root exudates of a hygrophyte.
Wherein, 1, collecting box; 2. a collection cover; 3. a first channel; 4. a sample injection needle tube; 5. a sample outlet needle tube; 6. a notch; 7. mounting grooves; 8. a tenon; 9. a joint; 10. a joint groove; 11. a head partition plate; 12. a tail baffle plate; 13. a solution isolation chamber outside the head; 14. a tail external solution isolation chamber; 15. a second channel; 16. collecting tank; 17. a sample inlet hole; 18. a sample outlet; 19. a rubber plug; 20. a base; 21. plant root systems; 22. and (4) a culture medium.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate the understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and it will be apparent to those skilled in the art that various changes may be made without departing from the spirit and scope of the invention as defined and defined in the appended claims, and all matters produced by the invention using the inventive concept are protected.
As shown in fig. 1-2, an in-situ collecting device for the root exudates of hygrophytes is provided, which comprises a collecting box 1, wherein an opening is arranged at the top of the collecting box 1, a collecting cover 2 is arranged on the opening, and the collecting cover 2 is detachably and hermetically connected with the collecting box 1; the material of collecting lid 1 and collecting box 2 is transparent material, can choose transparent ya keli material for use, adopts 3D printing technique shaping, and transparent root system of being convenient for observe cultivates the condition, culture solution interpolation etc..
The collecting box 1 is internally filled with a root system culture medium for culturing plants; the head end of the collecting box 1 is provided with a first channel 3 for the plant root system 21 to pass through; the inner bottom surface of the collecting box 1 is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box 1 is higher than that of the inner bottom surface of the tail end of the collecting box 1; the collection cover 2 is hermetically provided with a sample injection needle tube 4 for injecting the culture solution into the collection box 1 and a sample outlet needle tube 5 for sucking out root exudates and the culture solution. Collect lid 2 and collect box 1 and can dismantle sealing connection, make things convenient for root system and culture medium to put into and take out, reduce the experimentation and harm the root system.
Preferably but not limitatively, the side wall of the tail end of the collecting box 1 is provided with a notch 6 with the height same as the thickness of the collecting cover 2; be provided with mounting groove 7 on the inner wall of collection box 1 opening part, the protruding tenon 8 that is provided with and matches with mounting groove 7 on the lateral wall of collecting lid 2, is provided with the sealing washer between mounting groove 7 and the tenon 8, and after tenon 8 and the 7 cooperation of mounting groove, the tail end of collecting lid 2 is located breach 6 and collects the upper surface parallel and level of lid 2 and the upper surface of collecting box 1. The collecting box 1 and the collecting cover 2 are respectively provided with a mounting groove 7 and a tenon 8, so that the collecting cover 2 can be opened or closed in a drawing mode, the root system and the culture medium can be conveniently put in or taken out, and the damage to the root system in the experimental process is reduced; and be provided with the sealing washer between mounting groove 7 and tenon 8, make after mounting groove 7 is closed collect box 1 and keep apart with the external world, it is sealed effectual, fine prevent the entering of outside solution, be applicable to the wet environment of giving birth to.
Specifically, a joint 9 is convexly arranged on the side face of the head end of the collecting cover 2, a joint groove 10 matched with the joint 9 is arranged on the top face of the head end of the collecting box 1, the joint 9 is arranged behind the joint groove 10, and the outer wall of the side face of the head end of the collecting cover 2 is contacted with the inner wall of the side face of the head end of the collecting box 1; the cross section of the first channel 3 is in a semicircular structure, the first channel 3 is arranged on the bottom surface of the joint groove 10, and the top of the first channel 3 is connected with the bottom surface of the joint groove 10.
Furthermore, a head partition plate 11 and a tail partition plate 12 are respectively arranged at the head end and the tail end of the collecting box 1, the head partition plate 11 and the inner side wall of the head end of the collecting box 1 enclose to form a head external solution isolation chamber 13, and the tail partition plate 12 and the inner side wall of the tail end of the collecting box 1 enclose to form a tail external solution isolation chamber 14;
the top surface of the head partition plate 11 is provided with a second channel 15 for the plant root system 21 to pass through, the cross section of the second channel 15 is in a semicircular structure, and the top of the second channel 15 is connected with the top surface of the head partition plate 11;
the top surfaces of the head partition plate 11 and the tail partition plate 12 are provided with sealing gaskets for contacting with the lower end surface of the collecting box 1. The two ends of the mounting box are respectively provided with a head external solution isolation chamber 13 and a tail external solution isolation chamber 14, when external solution enters the collecting box 1, the external solution enters the head external solution isolation chamber 13 or the tail external solution isolation chamber 14 at first to form a first barrier, so that plant secretion solution in the collecting box 1 is prevented from being polluted. After the external solution isolation chamber is filled, the sealing gasket forms a second barrier to prevent external solution from entering. The cephalad ambient solution compartment 13 and caudal ambient solution compartment 14 may also be used as an indicator to determine whether the exudate is contaminated. When the head external solution isolation chamber 13 and the tail external solution isolation chamber 14 are filled with external solution, the secretion is polluted at a great risk, and the solution in the collector can be abandoned and collected again.
Collect interior bottom surface of box 1 and go up the undercut and be provided with collecting vat 16, collecting vat 16 is the big triangular prism structure of head end little tail end, and collecting vat 16's head end orientation is collected box 1's head end terminal surface and is met with head baffle 11, and collecting vat 16's tail end orientation is collected box 1's tail end and is met with afterbody baffle 12. The collecting tank 16 is a triangular prism structure with a small head end and a big tail end, so that the secretion of the plant root system and the culture solution can be collected more conveniently and thoroughly.
The head end of the collecting cover 2 is provided with a sample inlet hole 17, the tail end of the collecting cover is provided with a sample outlet hole 18, the sample inlet hole 17 and the sample outlet hole 18 are both internally provided with a rubber plug 19, and the outer walls of the two rubber plugs 19 are respectively contacted with the inner walls of the sample inlet hole 17 and the sample outlet hole 18; the sample injection needle tube 4 penetrates through the rubber plug 19 in the sample injection hole 17, and the end part of the sample injection needle tube is flush with the lower surface of the rubber plug 19, so that the root system is prevented from being damaged by the bottom of the poplar needle tube; the sample outlet needle tube 5 penetrates through the rubber plug 19 in the sample outlet hole 18, and the end part of the sample outlet needle tube is located at the lowest position of the collecting groove 16, so that the plant root secretion and the culture solution are collected more conveniently and completely.
Further, the bottom of the collecting box 1 is provided with a base 20 with legs. The base 20 is provided to facilitate the installation and fixation of the whole collecting device.
The invention also provides a collecting method of the in-situ collecting device for the hygrophyte root exudates, which is characterized by comprising the following steps:
step 1: digging and cleaning a complete plant root system 21, placing the plant root system 21 in the collecting box 1, and placing a root system main rod of the plant outside the collecting box 1 by penetrating through the first channel 3 and the second channel 15;
step 2: filling a culture medium into the mounting box to enable the culture medium to cover the plant root system 21; the culture medium may be vermiculite.
And step 3: closing the mounting cover to enable the collecting box 1 to be in an isolated and sealed state relative to the outside;
and 4, step 4: a sample injection needle tube 4 and a sample discharge needle tube 5 are respectively inserted into the mounting cover, the end part of the sample injection needle tube 4 is flush with the lower surface of the rubber plug 19, and the end part of the sample discharge needle tube 5 is positioned at the lowest position of the collecting groove 16;
and 5: placing the collecting box 1 and the collecting cover 2 in a plant root system acoustic environment, and covering black cloth on the collecting box 1 and the collecting cover 2 if the plant root system is a terrestrial plant root system; if the plant root is the aquatic plant root, the collection box 1 and the collection cover 2 are not covered with black cloth; injecting a preset amount of culture solution into the installation box by the sample injection needle tube 4, culturing the plant root system 21 for a preset time, and then entering the step 6; the culture solution is a carbon-free mineral solution matched with the primary environment of the plant root system 21;
step 6: and (3) washing the plant root system 21: extracting the culture solution from the sample outlet needle tube 5, injecting new culture solution into the sample inlet needle tube 4 until the preset times, and then entering the step 7; extracting culture solution, adding new culture solution, washing for 3-5 times, and eliminating interference.
And 7: culturing the plant root system 21 for a preset time, extracting the plant root system secretion solution by the sample outlet needle tube 5, and analyzing to obtain the carbon concentration of the plant root system secretion solution;
and 8: opening the mounting cover to take out the plant root system 21, and performing live root scanning on the plant root system 21 to obtain root system length, surface area data and dried root dry weight data;
and step 9: and in the step 7, obtaining the root secretion amount of the plant root system 21 generated within the preset time according to the extracted plant root secretion solution, the carbon concentration of the plant root secretion solution, the root length and surface area data in the step 8 and the dry weight data of the dried root.
Step 6 further comprises: the frequency of washing the plant root system 21 is three times; inject into the fixed Ma of culture solution volume, extract root system secretion solution volume N1 for the first time, add fixed volume culture solution Ma after the extraction, extract root system secretion solution volume N1 for the second time, adding fixed volume culture solution Ma, extract root system secretion solution volume N1 for the third time, ensure that plant roots washes more clean thoroughly, avoid influencing in the follow-up step, acquire the precision of plant roots secretion solution volume.
Step 9 further comprises: the amount of root exudates generated by the plant root system 21 in the preset time is calculated according to the following formula:
C＝K×M
wherein C is the root exudate amount, K is the carbon concentration of the plant root exudate solution, and M is the total volume of the extracted plant root exudate solution;
and (3) calculating the root secretion amount of the root system per hour in unit length, wherein the calculation formula is as follows:
X＝(K×M)/(T×L)；
wherein X is the root secretion amount of the root system per hour in unit length, T is preset time, and L is the length of the root system;
and (3) calculating the root secretion amount of the root system per hour on the unit surface area, wherein the calculation formula is as follows:
Y＝(K×M)/(T×S)；
wherein Y is the root secretion amount of the root system per hour on unit surface area, and S is the surface area of the root system;
and (3) calculating the root secretion amount of the root system per hour by unit dry weight, wherein the calculation formula is as follows:
Z＝(K×M)/(T×B)；
wherein Z is the root secretion amount of the root system per hour in unit dry weight, and B is the dry weight of the dried root system.
Claims (10)
1. The in-situ collecting device for the root exudates of the hygrophytes is characterized by comprising a collecting box, wherein the top of the collecting box is provided with an opening, a collecting cover is arranged on the opening, and the collecting cover is detachably and hermetically connected with the collecting box; the collecting cover and the collecting box are made of transparent materials;
the inside of the collecting box is filled with a root system culture medium for culturing plants;
the head end of the collecting box is provided with a first channel for the plant root system to pass through; the inner bottom surface of the collecting box is an inclined surface, and the height of the inner bottom surface of the head end of the collecting box is higher than that of the inner bottom surface of the tail end of the collecting box;
the collection cover is hermetically provided with a sample injection needle tube used for injecting the culture solution into the collection box and a sample outlet needle tube used for sucking the root secretion and the culture solution.
2. The in-situ collecting device for the root exudates of wet plants according to claim 1, wherein the side wall of the tail end of the collecting box is provided with a notch with the height same as the thickness of the collecting cover;
be provided with the mounting groove on the inner wall of collection box opening part, the lateral wall of collecting the lid go up the protruding be provided with the tenon that the mounting groove matches is provided with the sealing washer between mounting groove and the tenon, tenon and mounting groove cooperation back, the tail end of collecting the lid is located just collect the upper surface of lid and the upper surface parallel and level of collecting the box in the breach.
3. The in-situ collecting device for the root exudates of hygrophytes according to claim 2, wherein the side surface of the collecting cover head end is convexly provided with a joint, the top surface of the collecting box head end is provided with a joint groove matched with the joint, and after the joint is arranged in the joint groove, the outer wall of the side surface of the collecting cover head end is contacted with the inner wall of the side surface of the collecting box head end; the cross section of the first channel is of a semicircular structure, the first channel is arranged on the bottom surface of the joint groove, and the top of the first channel is connected with the bottom surface of the joint groove.
4. The in-situ collecting device for the root exudates of wet plants according to claim 2, wherein the head end and the tail end of the collecting box are respectively provided with a head partition plate and a tail partition plate, the head partition plate and the inner side wall of the head end of the collecting box enclose a head external solution isolation chamber, and the tail partition plate and the inner side wall of the tail end of the collecting box enclose a tail external solution isolation chamber;
the top surface of the head partition plate is provided with a second channel for the plant root system to pass through, the cross section of the second channel is in a semicircular structure, and the top of the second channel is connected with the top surface of the head partition plate;
the top surfaces of the head partition plate and the tail partition plate are respectively provided with a sealing gasket which is used for being in contact with the lower end surface of the collecting box.
5. The in-situ collecting device for the exudate from the root of a hygrophyte plant as claimed in claim 4, wherein the inner bottom surface of the collecting box is provided with a collecting tank which is of a triangular prism structure with a small head end and a big tail end, the head end of the collecting tank faces the head end surface of the collecting box and is connected with the head partition plate, and the tail end of the collecting tank faces the tail end of the collecting box and is connected with the tail partition plate.
6. The in-situ collecting device for the root exudates of hygrophytes according to claim 5, wherein the collecting cover is provided with a sample inlet hole at the head end and a sample outlet hole at the tail end, the sample inlet hole and the sample outlet hole are both internally provided with a rubber plug, and the outer walls of the two rubber plugs are respectively contacted with the inner walls of the sample inlet hole and the sample outlet hole; the sample injection needle tube penetrates through the rubber plug in the sample injection hole, and the end part of the sample injection needle tube is flush with the lower surface of the rubber plug; the sample outlet needle tube penetrates through the rubber plug in the sample outlet hole, and the end part of the sample outlet needle tube is located at the lowest position of the collecting groove.
7. The in-situ collecting device for the root exudates of the hygrophyte according to any one of claims 1 to 6, wherein a base with supporting legs is arranged at the bottom of the collecting box.
8. The collecting method of the in-situ collecting device for the root exudates of the hygrophyte according to any one of the claims 1 to 7, which comprises the following steps:
step 1: digging and cleaning a complete plant root system, placing the plant root system in a collecting box, and placing a root system main rod of the plant outside the collecting box through a first channel and a second channel;
step 2: filling a culture medium into the mounting box, and enabling the culture medium to cover the plant root system;
and step 3: closing the mounting cover to enable the collecting box to be in an isolated and sealed state relative to the outside;
and 4, step 4: a sample injection needle tube and a sample discharge needle tube are respectively inserted into the mounting cover, the end part of the sample injection needle tube is flush with the lower surface of the rubber plug, and the end part of the sample discharge needle tube is positioned at the lowest position of the collecting groove;
and 5: putting the collecting box and the collecting cover into the original sound environment of the plant root system, and covering black cloth on the collecting box and the collecting cover if the plant root system is a terrestrial plant root system; if the plant root is the aquatic plant root, the collecting box and the collecting cover are not covered with black cloth; injecting a preset amount of culture solution into the installation box by the sample injection needle tube, culturing the plant root system for a preset time, and then entering the step 6;
step 6: and (3) washing the plant root system: extracting the culture solution from the sample outlet needle tube, injecting new culture solution into the sample inlet needle tube until the times are preset, and then entering the step 7;
and 7: culturing the plant root system for a preset time, extracting the plant root system secretion solution through a sample outlet needle tube, and analyzing to obtain the carbon concentration of the plant root system secretion solution;
and 8: opening the mounting cover to take out the plant root system, and performing live root scanning on the plant root system to obtain root system length, surface area data and dried root dry weight data;
and step 9: and 7, obtaining the root secretion amount of the plant root system generated in the preset time according to the extracted plant root secretion solution, the carbon concentration of the plant root secretion solution, the root length and surface area data in the step 8 and the dry weight data of the dried root.
9. The method for collecting the in-situ wet plant root exudate (SOS) collecting device of claim 8, wherein the step 6 further comprises the following steps: the frequency of washing the plant root system is three times; injecting a fixed volume of culture solution Ma, extracting the volume of root exudate solution N1 for the first time, adding the fixed volume of culture solution Ma after extraction, extracting the volume of root exudate solution N1 for the second time, adding the fixed volume of culture solution Ma, and extracting the volume of root exudate solution N1 for the third time;
the step 7 further comprises: the times of extracting the plant root secretion solution are three times, and the volume of the first extracted plant root secretion solution is M1The culture solution of Ma capacity is injected into the mounting box after the extraction, and the volume of extracting plant roots secretion solution for the second time is M2, injects the culture solution of Ma capacity into the mounting box after the extraction, and the volume of extracting plant roots secretion solution for the third time is M3, injects the culture solution of Ma capacity into the mounting box after the extraction, and the total volume of extracting plant roots secretion solution is M, and M is M1+ M2+ M3.
10. The method for collecting the in-situ wet plant root exudate (SOS) collecting device of claim 9, wherein the step 9 further comprises: calculating the root secretion amount of the plant root system generated in the preset time, wherein the calculation formula is as follows:
C＝K×M
wherein C is the root exudate amount, K is the carbon concentration of the plant root exudate solution, and M is the total volume of the extracted plant root exudate solution;
and (3) calculating the root secretion amount of the root system per hour in unit length, wherein the calculation formula is as follows:
X＝(K×M)/(T×L)；
wherein X is the root secretion amount of the root system per hour in unit length, T is preset time, and L is the length of the root system;
and (3) calculating the root secretion amount of the root system per hour on the unit surface area, wherein the calculation formula is as follows:
Y＝(K×M)/(T×S)；
wherein Y is the root secretion amount of the root system per hour on unit surface area, and S is the surface area of the root system;
and (3) calculating the root secretion amount of the root system per hour by unit dry weight, wherein the calculation formula is as follows:
Z＝(K×M)/(T×B)；
wherein Z is the root secretion amount of the root system per hour in unit dry weight, and B is the dry weight of the dried root system.
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Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5524495A (en) * | 1992-06-22 | 1996-06-11 | Vortox Company | Liquid sampling devices and method |
| WO2000007437A1 (en) * | 1998-08-06 | 2000-02-17 | Rutgers, The State University Of New Jersey | A method of identifying and recovering products exuded from a plant |
| JP2001091420A (en) * | 1999-09-20 | 2001-04-06 | Hitachi Ltd | Sample container |
| KR20120016707A (en) * | 2010-08-17 | 2012-02-27 | 박치열 | The extract and the method extracting the extract from the bark of a tree, the root plant, the resin of sweet potato |
| CN202471451U (en) * | 2011-11-05 | 2012-10-03 | 中国科学院水利部成都山地灾害与环境研究所 | In-situ plant root exudate collecting device |
| KR101374601B1 (en) * | 2012-10-16 | 2014-03-17 | 한국식품연구원 | Basket for gathering fruit |
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN204948887U (en) * | 2015-08-20 | 2016-01-13 | 广东省农业科学院作物研究所 | Plant roots's device is surveyd to mobilizable normal position |
| CN205320700U (en) * | 2016-01-29 | 2016-06-22 | 四川农业大学 | Three -dimensional plant cultivation device |
| CN205408770U (en) * | 2016-02-15 | 2016-08-03 | 四川农业大学 | Hothouse plants cultivation device |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN106053135A (en) * | 2016-05-10 | 2016-10-26 | 中国科学院成都生物研究所 | A grassland herbal plant root exudate in-situ collecting method |
| CN207964788U (en) * | 2018-04-04 | 2018-10-12 | 四川农业大学 | A kind of root exudates collection device suitable for spacing sleeve cropping system |
| CN208425281U (en) * | 2018-07-05 | 2019-01-25 | 中国农业科学院作物科学研究所 | Plant culture and root exudates collection device |
| CN110208047A (en) * | 2019-06-21 | 2019-09-06 | 河南科技学院 | A kind of simple and efficient method for collecting wheat root flow liquid |
| WO2019180722A1 (en) * | 2018-03-22 | 2019-09-26 | Yeda Research And Development Co. Ltd | Methods and system for stimulating root exudation in plants |
| KR20200046977A (en) * | 2018-10-26 | 2020-05-07 | 농업회사법인(주)지노팜 | Recirculating constant temperature chamber apparatus for the cultivation of green bean sprouts or hydroponic plants |
| CN212520140U (en) * | 2020-04-16 | 2021-02-12 | 吉林省林业科学研究院 | Plant root system growth observation window box |
| CN214952401U (en) * | 2021-06-01 | 2021-11-30 | 河南大学 | Woody plant root exudate normal position collection device |
-
2021
- 2021-12-28 CN CN202111631773.6A patent/CN114375695B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5524495A (en) * | 1992-06-22 | 1996-06-11 | Vortox Company | Liquid sampling devices and method |
| WO2000007437A1 (en) * | 1998-08-06 | 2000-02-17 | Rutgers, The State University Of New Jersey | A method of identifying and recovering products exuded from a plant |
| JP2001091420A (en) * | 1999-09-20 | 2001-04-06 | Hitachi Ltd | Sample container |
| KR20120016707A (en) * | 2010-08-17 | 2012-02-27 | 박치열 | The extract and the method extracting the extract from the bark of a tree, the root plant, the resin of sweet potato |
| CN202471451U (en) * | 2011-11-05 | 2012-10-03 | 中国科学院水利部成都山地灾害与环境研究所 | In-situ plant root exudate collecting device |
| KR101374601B1 (en) * | 2012-10-16 | 2014-03-17 | 한국식품연구원 | Basket for gathering fruit |
| CN204948887U (en) * | 2015-08-20 | 2016-01-13 | 广东省农业科学院作物研究所 | Plant roots's device is surveyd to mobilizable normal position |
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN205320700U (en) * | 2016-01-29 | 2016-06-22 | 四川农业大学 | Three -dimensional plant cultivation device |
| CN205408770U (en) * | 2016-02-15 | 2016-08-03 | 四川农业大学 | Hothouse plants cultivation device |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN106053135A (en) * | 2016-05-10 | 2016-10-26 | 中国科学院成都生物研究所 | A grassland herbal plant root exudate in-situ collecting method |
| WO2019180722A1 (en) * | 2018-03-22 | 2019-09-26 | Yeda Research And Development Co. Ltd | Methods and system for stimulating root exudation in plants |
| CN207964788U (en) * | 2018-04-04 | 2018-10-12 | 四川农业大学 | A kind of root exudates collection device suitable for spacing sleeve cropping system |
| CN208425281U (en) * | 2018-07-05 | 2019-01-25 | 中国农业科学院作物科学研究所 | Plant culture and root exudates collection device |
| KR20200046977A (en) * | 2018-10-26 | 2020-05-07 | 농업회사법인(주)지노팜 | Recirculating constant temperature chamber apparatus for the cultivation of green bean sprouts or hydroponic plants |
| CN110208047A (en) * | 2019-06-21 | 2019-09-06 | 河南科技学院 | A kind of simple and efficient method for collecting wheat root flow liquid |
| CN212520140U (en) * | 2020-04-16 | 2021-02-12 | 吉林省林业科学研究院 | Plant root system growth observation window box |
| CN214952401U (en) * | 2021-06-01 | 2021-11-30 | 河南大学 | Woody plant root exudate normal position collection device |
Non-Patent Citations (7)
| Title |
|---|
| 丁爱芳: "新型根系分泌物采样装置的介绍与应用", 《土壤通报》 * |
| 杨丽娟: "几种有毒植物提取液对落叶松鞘蛾幼虫的毒杀作用", 《通化师范学院学报》 * |
| 涂书新等: "植物根系分泌物研究方法评述", 《生态环境学报》 * |
| 王世襄等: "《明式家具萃珍》", 31 January 1997 * |
| 王占义等: "一种新型根系分泌物收集装置与收集方法的介绍", 《土壤学报》 * |
| 郝大程等: "基于分子生物学和基因组学的植物根际微生物研究", 《微生物学通报》 * |
| 陈冬明等: "放牧强度和短期休牧对青藏高原东部高寒草甸优势物种根系分泌速率的影响", 《应用与环境生物学报》 * |
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