CN101206210A - Analog system device for detecting crop rhizosphere respiration and making plant - Google Patents
Analog system device for detecting crop rhizosphere respiration and making plant Download PDFInfo
- Publication number
- CN101206210A CN101206210A CNA2006101632733A CN200610163273A CN101206210A CN 101206210 A CN101206210 A CN 101206210A CN A2006101632733 A CNA2006101632733 A CN A2006101632733A CN 200610163273 A CN200610163273 A CN 200610163273A CN 101206210 A CN101206210 A CN 101206210A
- Authority
- CN
- China
- Prior art keywords
- gas
- transparent outer
- outer container
- sampling
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Cultivation Of Plants (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to an analog system device used to detect crop rhizosphere respiration and mark plant in the agricultural production technical field. The invention, which improves the prior analog system device used to detect crop rhizosphere respiration and mark plant, consists of a PVC pot 2, a transparent outer box 3, a water tank 4, a gas sampling box 6, a sampling plug 7, an isolation pipe 9 and a gas injection plug 10; meanwhile, the invention improves detection precision and increases detection efficiency; moreover, during collecting soil respiration gas sample, the invention can complete <13> C mark on the aboveground part of crop, thereby integrating gas-collecting process and marking process; thus, the invention can finally obtain detection result of high precision to meet the requirements of scientific research better.
Description
Technical field
The invention belongs to field of agricultural production technologies.Be that a kind of detection crop rhizosphere is breathed and mark plant analog system device, can widespread use in atmosphere-crop-soil simulation system.
Technical background
A kind of detection crop rhizosphere is breathed and mark plant analog system device, is the fixedly CO in the atmosphere of modeling effort crop photosynthesis effect
2Distribution and rhizosphere respiration CO at crop plant, rhizosphere respiration
2The system and device that emission flux combines together.Its major function is that soil and atmosphere are separated.In the past, the mensuration of people's heavy soil respiratory rate is breathed attention inadequately to crop rhizosphere, and detection means falls behind, and error is big, and precision is low.Now, along with the progress of science and technology and the increase of atmosphere percent of greenhouse gases, people update the distribution research method and the means of crop photosynthesis product, and people recognize the importance that crop rhizosphere is breathed clear-headedly, and its decision crop is the CO in the atmosphere fixedly
2Again be discharged into ability in the atmosphere by rhizosphere respiration.So the researchist of greenhouse gases science both domestic and external aspect pays much attention to the research that crop rhizosphere is breathed.Rhizosphere respiration is as the fixing CO of crop photosynthesis effect
2, to atmospheric discharging CO
2The core component of process form focus and breach into the greenhouse gases research work, and it separates soil and relatively lags behind with the atmosphere interface method, few people study.
The general employing of traditional method is used less than the sampling box of basin alms bowl surface area at basin alms bowl soil face and is measured soil respiration, carries out mark with the transparent outer container that can't isolate soil and atmosphere interface, and disturbing factor is more in the measuring process, waste
13C-CO
2, error is big, make some less soil respiration differences detect less than, part is out in the cold.The present invention is exactly at above shortcoming, improve and perfect, take to isolate the sampling box of native face and atmosphere interface and crop plant can be placed the transparent outer container in the case that crop soil respiration is measured, and combine together with the crop plant labeling process, the data that detected soil respiration speed and marked product are distributed are accurate, precision improves, and is present the most feasible effective way.
Summary of the invention
The present invention is from above technical background, a kind of detection crop rhizosphere is breathed and mark plant analog system device improves and perfect, inhale the process of exhaling and combine together measuring soil, obtain soil respiration speed accurately and photosynthate distribution rule with the process of mark photosynthate.
Key of the present invention is that native face-atmosphere is all isolated and the crop plant mark and the external world are isolated simultaneously.We have finished the present invention through repeatedly test.The present invention is made up of basin alms bowl 2, transparent outer container 3, tank 4, gas sampling case 6, sampling plug 7, isolated tube 9 and gas injection plug 10.So just can combine together, finally obtain accurate data and information measuring the process of rhizosphere respiration and the process of mark plant,, thus better meet the scientific research needs.Concrete operation method is according to the following steps:
A. early-stage preparations: plant plant in the isolated tube 9;
B. sealing: tank is added 300ml distilled water for 4 li, and the base of sealing sampling box 6 is with the junction of 704 silica gel, 8 sealing sampling boxes 6 with isolated tube 9;
C. mixing: with the logical sampling box 6 tops sampling of 60ml syringe plug, twitch piston 3-4 time back and forth, with the interior gas of mixing sampling box 6;
D. gas production: get the vacuum glass bottle that 20ml gas injects 18ml with syringe;
E. detect: measure with gas chromatography;
F. transparent outer container: Open Side Down that cover is gone up crop plant transparent outer container 3, and end opening is put into tank 4;
G. add label: extract 50ml with the 60m syringe
13C-CO
2, the place is injected in the transparent outer container 3 at transparent outer container gas injection plug;
H. carry out mark: add
13C-CO
2After, spray transparent outer container 3 with tap water, reduce the temperature in the transparent outer container 3.
Accompanying drawing 1 explanation
1, soil; 2, basin alms bowl; 3, transparent outer container; 4, tank; 5, crop plant; 6, gas sampling case; 7, sampling plug; 8,704 silica gel; 9, from pipe; 10 gas injection plugs.
Embodiment
The present invention (2005CB121101) is applied in the problem in state key fundamental research development plan (973 plan).In the carrying out of problem, used the present invention, according to method of operating described in the summary of the invention and step, actual detected has been carried out in plantation soybean soil respiration and photosynthate distribution, adopt conventional sense means efficient to improve 1.5 times more at present, precision improves more than 15%, has obtained effect preferably.
The present invention is easy and simple to handle, and the degree of accuracy height is practical.Further investigation to atmosphere-crop-soil carbon circulating analog is helpful, has the excellent popularization prospect.
Claims (1)
1. one kind is detected crop rhizosphere breathing and mark plant analog system device, it is characterized in that being made up of basin alms bowl (2), transparent outer container (3), tank (4), gas sampling case (6), sampling plug (7), isolated tube (9) and gas injection plug (10); Concrete operation method and step are as follows:
A. early-stage preparations: in isolated tube (9), planting plant;
B. sealing: tank (4) lining is added 300ml distilled water, and the base of sealing sampling box (6) is with the junction of 704 silica gel (8) sealing sampling boxes (6) with isolated tube (9);
C. mixing:, twitch piston 3-4 time back and forth, with the interior gas of mixing sampling box (6) with logical sampling box (6) the top sampling of 60ml syringe plug;
D. gas production: get the vacuum glass bottle that 20ml gas injects 18ml with syringe;
E. detect: measure with gas chromatography;
F. transparent outer container: Open Side Down that cover is gone up crop plant transparent outer container (3), and end opening is put into tank (4);
G. add label: extract 50ml with the 60m syringe
13C-CO
2, the place is injected in the transparent outer container (3) at transparent outer container gas injection plug;
H. carry out mark: add
13C-CO
2After, spray transparent outer container (3) with tap water, reduce the temperature in the transparent outer container (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101632733A CN101206210A (en) | 2006-12-18 | 2006-12-18 | Analog system device for detecting crop rhizosphere respiration and making plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101632733A CN101206210A (en) | 2006-12-18 | 2006-12-18 | Analog system device for detecting crop rhizosphere respiration and making plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101206210A true CN101206210A (en) | 2008-06-25 |
Family
ID=39566573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006101632733A Pending CN101206210A (en) | 2006-12-18 | 2006-12-18 | Analog system device for detecting crop rhizosphere respiration and making plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101206210A (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053145A (en) * | 2010-11-03 | 2011-05-11 | 中国科学院新疆生态与地理研究所 | In-situ automatic measurement method of CO2 of root system |
| CN101776542B (en) * | 2009-01-11 | 2011-10-19 | 中国科学院寒区旱区环境与工程研究所 | Soil-plant system gas exchange continuous measurement acquisition device |
| CN101363825B (en) * | 2008-08-06 | 2011-12-14 | 西北农林科技大学 | Device for dynamically detecting carbon element loss of root system of plant |
| CN104221740A (en) * | 2014-10-11 | 2014-12-24 | 中国环境科学研究院 | Device and method for continuously and quantitatively labeling plants |
| CN104569135A (en) * | 2015-01-14 | 2015-04-29 | 浙江大学 | Device for measuring nutrition contribution of amino acid in soil on plant growth and application |
| CN104833739A (en) * | 2015-05-13 | 2015-08-12 | 中国科学院亚热带农业生态研究所 | Method and device for distinguishing rice respiration, rice rhizospheric respiration and non-rhizospheric respiration |
| CN106248444A (en) * | 2016-08-26 | 2016-12-21 | 吕文强 | Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof |
| CN107942036A (en) * | 2017-11-16 | 2018-04-20 | 中国科学院亚热带农业生态研究所 | A kind of method and device of isotope labeled in situ Methane in Soils oxidation bacteria |
| CN110095589A (en) * | 2019-06-21 | 2019-08-06 | 福建师范大学地理研究所 | A kind of ground, underground Carbon flux synchronous experiments equipment |
| CN110687247A (en) * | 2019-10-30 | 2020-01-14 | 江西农业大学 | No-soil body disturbance collection and determination of root system and soil CO2Breath test device |
| CN111751500A (en) * | 2020-07-06 | 2020-10-09 | 吉林建筑大学 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
| CN111929117A (en) * | 2020-09-29 | 2020-11-13 | 中国农业科学院农业环境与可持续发展研究所 | Methane monitoring device |
| CN113063903A (en) * | 2021-04-25 | 2021-07-02 | 湖南杂交水稻研究中心 | Adjustable isotope labeling device for plant photosynthesis |
-
2006
- 2006-12-18 CN CNA2006101632733A patent/CN101206210A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363825B (en) * | 2008-08-06 | 2011-12-14 | 西北农林科技大学 | Device for dynamically detecting carbon element loss of root system of plant |
| CN101776542B (en) * | 2009-01-11 | 2011-10-19 | 中国科学院寒区旱区环境与工程研究所 | Soil-plant system gas exchange continuous measurement acquisition device |
| CN102053145B (en) * | 2010-11-03 | 2013-06-05 | 中国科学院新疆生态与地理研究所 | In-situ automatic measurement method of CO2 of root system |
| CN102053145A (en) * | 2010-11-03 | 2011-05-11 | 中国科学院新疆生态与地理研究所 | In-situ automatic measurement method of CO2 of root system |
| CN104221740A (en) * | 2014-10-11 | 2014-12-24 | 中国环境科学研究院 | Device and method for continuously and quantitatively labeling plants |
| CN104569135B (en) * | 2015-01-14 | 2017-06-13 | 浙江大学 | Determine device and purposes that soil amino acid is contributed plant growth nutrition |
| CN104569135A (en) * | 2015-01-14 | 2015-04-29 | 浙江大学 | Device for measuring nutrition contribution of amino acid in soil on plant growth and application |
| CN104833739A (en) * | 2015-05-13 | 2015-08-12 | 中国科学院亚热带农业生态研究所 | Method and device for distinguishing rice respiration, rice rhizospheric respiration and non-rhizospheric respiration |
| CN106248444A (en) * | 2016-08-26 | 2016-12-21 | 吕文强 | Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof |
| CN107942036A (en) * | 2017-11-16 | 2018-04-20 | 中国科学院亚热带农业生态研究所 | A kind of method and device of isotope labeled in situ Methane in Soils oxidation bacteria |
| CN110095589A (en) * | 2019-06-21 | 2019-08-06 | 福建师范大学地理研究所 | A kind of ground, underground Carbon flux synchronous experiments equipment |
| CN110687247A (en) * | 2019-10-30 | 2020-01-14 | 江西农业大学 | No-soil body disturbance collection and determination of root system and soil CO2Breath test device |
| CN111751500A (en) * | 2020-07-06 | 2020-10-09 | 吉林建筑大学 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
| CN111929117A (en) * | 2020-09-29 | 2020-11-13 | 中国农业科学院农业环境与可持续发展研究所 | Methane monitoring device |
| CN113063903A (en) * | 2021-04-25 | 2021-07-02 | 湖南杂交水稻研究中心 | Adjustable isotope labeling device for plant photosynthesis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101206210A (en) | Analog system device for detecting crop rhizosphere respiration and making plant | |
| Chasar et al. | Radiocarbon and stable carbon isotopic evidence for transport and transformation of dissolved organic carbon, dissolved inorganic carbon, and CH4 in a northern Minnesota peatland | |
| CN102732466B (en) | Method for culturing denitrifying bacterium and determining water body nitrate nitrogen isotope composition | |
| CN102053145A (en) | In-situ automatic measurement method of CO2 of root system | |
| CN102589923A (en) | Plant 13CO2 mark cultivation-root exudate collection integration device | |
| CN101377474A (en) | Method for identifying genuine Chinese medicine based on stability isotope element fingerprint pattern | |
| CN102440153B (en) | Pulse type device for conducting quantitative marking on plant with <13>CO2, and method for marking plant by utilizing same | |
| CN108776051A (en) | A kind of soil, plant evaporation steam isotope harvester | |
| Zhang et al. | Radiocarbon isotope technique as a powerful tool in tracking anthropogenic emissions of carbonaceous air pollutants and greenhouse gases: A review | |
| Liu et al. | Did an extensive forest ever develop on the Chinese Loess Plateau during the past 130 ka?: a test using soil carbon isotopic signatures | |
| CN202974977U (en) | Portable ecological hydrologic experiment and monitoring system | |
| CN206609782U (en) | A kind of edaphon respiration measuring device | |
| CN104833739B (en) | Method and device for distinguishing rice respiration, rice rhizospheric respiration and non-rhizospheric respiration | |
| CN205139009U (en) | Quick detection device of portable soil nutrients | |
| CN207318164U (en) | A kind of continuous carbon nitrogen isotope double labelling device of more time point samplings | |
| CN101934157A (en) | Device and method for extracting water content of soil sample | |
| CN106248444A (en) | Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof | |
| CN204831861U (en) | Gassing collection system is separated naturally to shale gas well rock core | |
| CN103698476A (en) | Isotopic tracer method for determining carbon sources and carbon sinks of perennial vegetation in situ | |
| CN202631497U (en) | Controllable correction environment-based measurement device for atmospheric water isotope gradient and vertical fractionation process | |
| CN208270269U (en) | A kind of soil, plant evaporation steam isotope acquisition device | |
| CN113532961B (en) | U-shaped layered soil nitrous oxide gas collection method | |
| CN208579966U (en) | A kind of paddy field collection device of greenhouse | |
| CN104000857A (en) | Method for extracting common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating common traditional Chinese medicine through MALDI-TOF-MS | |
| CN204314112U (en) | A kind of Soil Trace Gases sampling static chamber with filtering function |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080625 |