CN210519655U - Arbuscular mycorrhizal fungi propagation device - Google Patents
Arbuscular mycorrhizal fungi propagation device Download PDFInfo
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- CN210519655U CN210519655U CN201921022057.6U CN201921022057U CN210519655U CN 210519655 U CN210519655 U CN 210519655U CN 201921022057 U CN201921022057 U CN 201921022057U CN 210519655 U CN210519655 U CN 210519655U
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Abstract
The utility model discloses a arbuscular mycorrhizal fungi expands numerous device, including the transparent box that has ventilation assembly and install the plant cultivation subassembly in the transparent box, transparent box is connected with the moisturizing subassembly that is arranged in supplementing moisture to the plant cultivation subassembly, the internal steam condensation that is equipped with of transparent box retrieves the subassembly, the subassembly is retrieved to steam condensation includes the water collecting hopper and locates in the transparent box inner chamber and from the downward convex spherical crown face of transparent box inner chamber roof, the water collecting hopper is located under the middle part of spherical crown face, the water collecting hopper is cultivateed the subassembly through a connecting tube and is linked together and put the log raft of collecting to the plant cultivation subassembly. The utility model has the advantages of simple structure, easy manufacture, stable and reliable work, and reduced workload.
Description
Technical Field
The utility model belongs to the technical field of equipment is cultivated in the fungi propagation, especially, relate to a device is propagated to expand of arbuscular mycorrhizal fungi.
Background
With the rapid development of modern industry and urbanization process, modern agriculture is facing to increasingly serious ecological environment and resource tightening problems, wherein soil salinization, desertification and heavy metal pollution become important factors threatening agricultural production.
Arbuscular Mycorrhizal Fungi (AMF) is a special symbiotic fungus, widely exists in various ecological environments in nature, can form mycorrhiza with most plants on land and forms a reciprocal symbiotic relationship with host plants. After the AMF and the root system of the plant are combined, the drought resistance, salt resistance, high temperature resistance and disease resistance of the host plant can be improved, and meanwhile, the physical and chemical properties of the soil can be improved, and the yield of crops can be improved.
The arbuscular mycorrhizal fungi are widely distributed, and can form a huge hypha network system with the root system of the plant to enhance the stress resistance of the plant. Therefore, the AMF can be applied to vegetation recovery, mine restoration, soil fertility improvement and the like, and is expected to become a novel biological fertilizer for the fields of agricultural production, landscaping and the like.
At present, arbuscular mycorrhizal fungi cannot be purely cultured under in vitro conditions, and the life history of the arbuscular mycorrhizal fungi can be completed only after the arbuscular mycorrhizal fungi and host plants are symbiotic. The existing AMF microbial inoculum production methods comprise a plurality of methods, such as a potting culture method, a spray culture method, a glass bead chamber culture method and the like. The traditional pot culture method is continuously used up to now because of simple operation, reliable method and the like, but has the defects of about 4 months of culture in a constant-temperature and basically sterile environment, long culture period and troublesome maintenance. So far, an arbuscular mycorrhizal fungi propagation device which is easy to manufacture, simple in structure, energy-saving and efficient is lacked, so that the extensive popularization and application of the arbuscular mycorrhizal fungi in agriculture is hindered.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome the not enough of prior art existence, provide a simple structure, easily preparation, job stabilization is reliable, can reduce the arbuscular mycorrhizal fungi propagation device of work load.
In order to solve the technical problem, the utility model discloses a following technical scheme:
the utility model provides a arbuscular mycorrhizal fungi expands numerous device, including the transparent box that has the ventilation subassembly with install plant cultivation subassembly in the transparent box, transparent box is connected with the moisturizing subassembly that is arranged in supplementing moisture to plant cultivation subassembly, be equipped with steam condensation recovery subassembly in the transparent box, steam condensation recovery subassembly includes the water collecting hopper and locates in the transparent box inner chamber and from the downward convex spherical crown surface of transparent box inner chamber roof, the water collecting hopper is located under the middle part of spherical crown surface, the water collecting hopper is connected with plant cultivation subassembly through a connecting tube and discharges the water of collecting to plant cultivation subassembly.
As a further improvement of the above technical solution:
the water supplementing assembly comprises a water storage tank, a water pump, a water pipe and an atomizing nozzle, wherein the atomizing nozzle is arranged right above the plant cultivation assembly in the transparent box body and is communicated with the water storage tank through the water pump and the water pipe.
The ventilation assembly comprises two fans which are respectively arranged at the upper parts of the two sides of the transparent box body.
The plant cultivation component comprises a seedbed arranged at the bottom of the transparent box body and a seedbed mattress arranged below the seedbed.
The arbuscular mycorrhizal fungi propagation expanding device is provided with a nutrient solution adding assembly used for adding nutrient solution to the plant cultivation assembly, the nutrient solution adding assembly comprises a nutrient solution box and a nutrient solution pump, an inlet of the nutrient solution pump is connected with the nutrient solution box, and an outlet of the nutrient solution pump is connected to the plant cultivation assembly through a pipeline.
And the transparent box body is internally provided with an illuminating assembly, and the illuminating assembly comprises more than one fluorescent lamp tube positioned above the plant cultivation assembly.
The transparent box is internally provided with a sensor which is connected with a first miniature intelligent control and Internet of things monitor arranged outside the transparent box.
Moisturizing subassembly and ventilation subassembly all with first miniature intelligent control is connected with thing networking monitor.
The arbuscular mycorrhizal fungi propagation device is further provided with a second miniature intelligent control and internet of things monitor, and the nutrient solution adding assembly is connected with the second miniature intelligent control and internet of things monitor.
The arbuscular mycorrhizal fungi propagation device is further provided with a third miniature intelligent control and Internet of things monitor, and the lighting assembly is connected with the third miniature intelligent control and Internet of things monitor.
Compared with the prior art, the utility model has the advantages of:
the utility model discloses an assembly is retrieved in condensation of steam to be equipped with in the transparent box body of arbuscular mycorrhizal fungi propagation device, and the spherical crown face of assembly is retrieved in the condensation of steam and from the downward protrusion of transparent box inner chamber roof, does benefit to the condensation of transparent box internal steam to concentrate and flow into the water catch bowl and discharge, this assembly is retrieved in condensation of steam that spherical crown face and water catch bowl combination has simple structure compactness, easily preparation, the reliable advantage of job stabilization. Simultaneously, the water collecting hopper discharges the collected water to the plant cultivation assembly, so that the water inside the transparent box body can be recycled, the water supplementing times are reduced, the workload is reduced, and the water consumption is reduced.
Drawings
FIG. 1 is a schematic structural diagram of an arbuscular mycorrhizal fungi propagation device.
Illustration of the drawings:
1. a transparent box body; 2. a water collecting hopper; 3. a spherical crown surface; 4. a water storage tank; 5. a water pump; 6. a water pipe; 7. an atomizing spray head; 8. a fan; 9. a seedbed; 10. a seedling mattress; 11. a nutrient solution tank; 12. a nutrient solution pump; 13. a fluorescent tube; 14. a sensor; 15. miniature intelligent control and thing networking monitor.
Detailed Description
The present invention will be described in further detail with reference to the following drawings and specific embodiments.
As shown in fig. 1, the arbuscular mycorrhizal fungi propagation expanding device of this embodiment includes transparent box 1 with ventilation assembly and the plant cultivation assembly installed in transparent box 1, transparent box 1 is connected with the moisturizing assembly that is used for replenishing moisture in the plant cultivation assembly, be equipped with the steam condensation recovery assembly in transparent box 1, the steam condensation recovery assembly includes water collecting hopper 2 and locates in transparent box 1 inner chamber and from transparent box 1 inner chamber roof downwardly convex spherical crown surface 3, water collecting hopper 2 is located under the middle part of spherical crown surface 3, water collecting hopper 2 is connected with the plant cultivation assembly through a connecting tube and discharges the water of collecting to the plant cultivation assembly. This arbuscular mycorrhizal fungi expands numerous device's transparent box 1 is equipped with steam condensation and retrieves the subassembly, and the spherical crown face 3 of steam condensation retrieves the subassembly and projects downwards from 1 inner chamber roof of transparent box, does benefit to the condensation of steam in the transparent box 1 to concentrate and flow into water collecting bucket 2 and discharge, this spherical crown face 3 and the steam condensation of water collecting bucket 2 combination retrieve the subassembly and have simple structure compactness, easily preparation, the reliable and stable advantage of work. Meanwhile, the water collecting hopper 2 discharges collected water to the plant cultivation assembly, so that the water inside the transparent box body 1 can be recycled, the water replenishing frequency is reduced, the workload is reduced, and the water consumption is reduced.
In this embodiment, the moisturizing subassembly includes storage water tank 4, water pump 5, water pipe 6 and atomizer 7, and atomizer 7 arranges directly over the subassembly is cultivateed to the plant in transparent box 1, and atomizer 7 passes through water pump 5 and water pipe 6 and storage water tank 4 intercommunication. This moisturizing subassembly can carry out automatic water supply, starts water pump 5 and can send the water pump in the storage water tank 4 to water pipe 6 to spout on the plant cultivation subassembly through atomizer 7.
In this embodiment, the ventilation assembly is two fans 8, and two fans 8 are respectively arranged at the upper parts of two sides of the transparent box body 1, so that the ventilation effect can be improved.
In this embodiment, the plant cultivation assembly includes a seedbed 9 disposed at the bottom of the transparent case 1 and a seedbed mat 10 disposed under the seedbed 9.
In this embodiment, the arbuscular mycorrhizal fungi expands numerous device and is equipped with the nutrient solution that is used for adding the nutrient solution to the plant cultivation subassembly and adds the subassembly, and the nutrient solution adds the subassembly and includes nutrient solution case 11 and nutrient solution pump 12, and nutrient solution pump 12's import links to each other with nutrient solution case 11, and nutrient solution pump 12's export is through pipe connection to plant cultivation subassembly. This nutrient solution adds subassembly can supply nutrient solution to the plant cultivation subassembly automatically, starts nutrient solution pump 12 and can pass through the pipeline pump with the nutrient solution in the nutrient solution case 11 and send to the plant cultivation subassembly. The nutrient solution used by the nutrient solution adding component is an improved Hoagland nutrient solution, and the preparation method of the improved Hoagland nutrient solution comprises the following steps: dissolving 0.945g of calcium nitrate, 0.506g of potassium nitrate, 0.08g of ammonium nitrate, 0.136g of monopotassium phosphate, 0.493g of magnesium sulfate, 2.5mL of ferric salt mother liquor and 5 mu L of 1000-time trace element mother liquor in ultrapure water, fixing the volume to 1L, and adjusting the pH value to 6.0 by using 1% by mass of potassium hydroxide; wherein, the ferric salt mother liquor is a solution obtained by dissolving 5.56g of ferrous sulfate and 7.46g of ethylene diamine tetraacetic acid in ultrapure water, and regulating the pH value to 5.5 by using 1M hydrochloric acid after the solution is dissolved for 1L; wherein the 1000-time microelement mother liquor is a solution obtained by dissolving 0.83g of potassium iodide, 6.2g of boric acid, 22.3g of manganese sulfate, 8.6g of zinc sulfate, 0.25g of sodium molybdate, 0.025g of copper sulfate and 0.025g of cobalt chloride in ultrapure water and fixing the volume to 1L.
In this embodiment, the transparent box body 1 is internally provided with the lighting assembly, and the lighting assembly can provide sunlight for plants under the conditions of low light and no light such as cloudy days or at night, and promotes the growth and development of plant roots. The lighting assembly comprises a plurality of fluorescent tubes 13 which are positioned above the plant cultivation assembly, and the fluorescent tubes 13 are arranged in parallel and at intervals, so that the light supplementing effect can be improved.
In this embodiment, transparent box 1 is inside to be equipped with a sensor 14, and sensor 14 is connected with one and locates the outside first miniature intelligent control of transparent box 1 and thing networking monitor. The environment information such as the temperature, the humidity, the illumination, the carbon dioxide concentration and the like in the transparent box body 1 can be monitored in real time, and the optimal environment in the transparent box body 1 can be adjusted and controlled conveniently and timely.
In this embodiment, moisturizing subassembly and ventilation subassembly all are connected with first miniature intelligent control and thing networking monitor, and the first miniature intelligent control of accessible and thing networking monitor control moisturizing subassembly and ventilation subassembly regularly mend water and regularly ventilate to can in time moisturizing and ventilate according to the environmental information of sensor 14 feedback.
In this embodiment, the device is propagated to arbuscular mycorrhizal fungi still is equipped with the miniature intelligent control of second and thing networking monitor, and nutrient solution adds the subassembly and is connected with the miniature intelligent control of second and thing networking monitor, and the miniature intelligent control of accessible second adds the subassembly with thing networking monitor control nutrient solution and regularly supplements the nutrient solution.
In this embodiment, the arbuscular mycorrhizal fungi propagation device is further provided with a third miniature intelligent control and internet of things monitor, the lighting assembly is connected with the third miniature intelligent control and internet of things monitor, the lighting assembly can be controlled to be turned on and turned off through the third miniature intelligent control and the internet of things monitor, and the lighting assembly can be turned on and turned off automatically in real time according to the illumination intensity by being matched with an illumination detector (such as the sensor 14).
In this embodiment, preferably, the first miniature intelligent control and internet of things monitor, the second miniature intelligent control and internet of things monitor, and the third miniature intelligent control and internet of things monitor are integrated into one control cabinet.
The method for carrying out the arbuscular mycorrhizal fungi propagation by adopting the arbuscular mycorrhizal fungi propagation device of the embodiment comprises the following steps:
1. sterilizing a substrate for seedling culture: river sand and vermiculite are mixed according to the proportion of 1: 1, sterilizing with steam at 121 deg.C for 2 hr, and air drying.
2. Seed disinfection: soaking four wild pea seeds in 10 vol% sodium hypochlorite solution for 2 min for disinfection, washing with sterile water for 2-3 times, soaking with 75% alcohol for 1 min, and washing with sterile water for 2-3 times to obtain disinfected seeds.
3. Sterilizing the seedbed 9: the seedbed 9 is cleaned, dried and disinfected by 75% alcohol for later use.
4. Sterilizing the device: all components in the whole device are sterilized by 75% alcohol and then are ready for use.
5. When sowing, firstly, the seedbed 9 is filled with the sterile substrate, then a proper amount of AMF microbial inoculum is uniformly layered on the seedbed, water is sprayed to wet the bottom of the seedbed 9 until the bottom of the seedbed is slightly soaked with water, the sowing mode is adopted to sow the sterilized seeds, and then a layer of sterile substrate with the thickness of 1-2mm is covered.
6. Culturing for about 4 months according to the temperature and humidity in a conventional temperature and humidity control device, randomly sampling, detecting the mycorrhiza infection rate of four wild peas, and obtaining the AMF microbial inoculum when the infection rate reaches 100%.
During the culture period of the four wild peas, the water supplementing component and the nutrient solution adding component can supplement water and nutrients to the roots of the four wild peas in an intermittent manner, promote the growth and development of the roots of the four wild peas, and adjust the times and the period of supplementing the nutrient solution according to the growth condition of plants.
During the cultivation period of four wild peas, the moisture content that the device can be retrieved to the steam condensation recovery subassembly moisture content because of fluorescent tube 13 shines for a long time and evaporates to the top and condense into the water droplet, during the mattress 10 of device bottom is flowed to in the water conservancy diversion of condensation, realize the inside moisture content cyclic utilization of device, reduce the consumption, retrieve the subassembly design for the spherical crown face with the steam condensation, can improve the efficiency of neat condensation recovery.
During the culture period of four wild peas, the sensor 14 and the miniature intelligent control and Internet of things monitor 15 can monitor the environmental information such as temperature, humidity, illumination, carbon dioxide concentration and the like in the device in real time, know the growth condition in real time and maintain in time.
During the cultivation period of the four wild peas, the lighting assembly can provide sunlight for plants under the conditions of no illumination such as at night and the like, and the growth and development of root systems of the four wild peas are promoted.
During the cultivation of four wild peas, utilize miniature intelligent control and thing networking monitor 15 to control fluorescent lamp pipe 13, fan 8, water pump 5 and nutrient solution pump 12, can realize the time control and the automated control of length, the ventilation requirement of illumination time, moisturizing, the interpolation nutrient solution according to real-time environment information in the device, when further simplifying operation flow, also can provide optimal environment for plant roots's development and the expanding propagation of arbuscular mycorrhizal fungi, also can realize simultaneously that wireless data gathers checks remote control.
The above description is only the preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. For those skilled in the art, the modifications and changes obtained without departing from the technical idea of the present invention shall be considered as the protection scope of the present invention.
Claims (10)
1. The utility model provides an arbuscular mycorrhizal fungi expands numerous device, includes transparent box (1) that has the ventilation subassembly and installs the plant cultivation subassembly in transparent box (1), transparent box (1) is connected with the moisturizing subassembly that is arranged in supplementing moisture to the plant cultivation subassembly, its characterized in that: be equipped with the steam condensation in transparent box (1) and retrieve the subassembly, the steam condensation is retrieved the subassembly and is included water catch bowl (2) and locate in transparent box (1) inner chamber and from transparent box (1) inner chamber roof convex spherical crown face (3) downwards, water catch bowl (2) are located under the middle part of spherical crown face (3), water catch bowl (2) are connected with the plant culture subassembly through a connecting tube and discharge the water of collecting to the plant culture subassembly.
2. The arbuscular mycorrhizal fungi propagation device of claim 1, wherein: the water supplementing assembly comprises a water storage tank (4), a water pump (5), a water pipe (6) and an atomizing nozzle (7), wherein the atomizing nozzle (7) is arranged above the plant culture assembly in the transparent box body (1) and is communicated with the water storage tank (4) through the water pump (5) and the water pipe (6).
3. The arbuscular mycorrhizal fungi propagation device of claim 1, wherein: the ventilation component comprises two fans (8) which are respectively arranged at the upper parts of the two sides of the transparent box body (1).
4. The arbuscular mycorrhizal fungi propagation device of claim 1, wherein: the plant cultivation component comprises a seedbed (9) arranged at the bottom of the transparent box body (1) and a seedbed mattress (10) arranged below the seedbed (9).
5. The arbuscular mycorrhizal fungi propagation device of claim 1, wherein: the arbuscular mycorrhizal fungi propagation expanding device is provided with a nutrient solution adding assembly used for adding nutrient solution to the plant culture assembly, the nutrient solution adding assembly comprises a nutrient solution box (11) and a nutrient solution pump (12), an inlet of the nutrient solution pump (12) is connected with the nutrient solution box (11), and an outlet of the nutrient solution pump (12) is connected to the plant culture assembly through a pipeline.
6. The arbuscular mycorrhizal fungi propagation device of claim 1, wherein: and an illuminating assembly is arranged in the transparent box body (1), and the illuminating assembly comprises more than one fluorescent lamp tube (13) positioned above the plant cultivation assembly.
7. The arbuscular mycorrhizal fungi propagation device according to any one of claims 1 to 6, wherein: transparent box (1) inside is equipped with a sensor (14), sensor (14) are connected with one and locate transparent box (1) outside first miniature intelligent control and thing networking monitor.
8. The arbuscular mycorrhizal fungi propagation device of claim 7, wherein: moisturizing subassembly and ventilation subassembly all with first miniature intelligent control is connected with thing networking monitor.
9. The arbuscular mycorrhizal fungi propagation device of claim 5, wherein: the arbuscular mycorrhizal fungi propagation device is further provided with a second miniature intelligent control and internet of things monitor, and the nutrient solution adding assembly is connected with the second miniature intelligent control and internet of things monitor.
10. The arbuscular mycorrhizal fungi propagation device of claim 6, wherein: the arbuscular mycorrhizal fungi propagation device is further provided with a third miniature intelligent control and Internet of things monitor, and the lighting assembly is connected with the third miniature intelligent control and Internet of things monitor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921022057.6U CN210519655U (en) | 2019-07-02 | 2019-07-02 | Arbuscular mycorrhizal fungi propagation device |
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| CN201921022057.6U CN210519655U (en) | 2019-07-02 | 2019-07-02 | Arbuscular mycorrhizal fungi propagation device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514710A (en) * | 2020-11-30 | 2021-03-19 | 山西农业大学 | Arbuscular mycorrhizal fungi continuous type propagation incubator |
| CN112753451A (en) * | 2021-01-19 | 2021-05-07 | 洛阳理工学院 | Breeding constant temperature incubator that agricultural technology research was used |
-
2019
- 2019-07-02 CN CN201921022057.6U patent/CN210519655U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514710A (en) * | 2020-11-30 | 2021-03-19 | 山西农业大学 | Arbuscular mycorrhizal fungi continuous type propagation incubator |
| CN112753451A (en) * | 2021-01-19 | 2021-05-07 | 洛阳理工学院 | Breeding constant temperature incubator that agricultural technology research was used |
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