CN104381105A - Method for quick rooting of divaricate velvetplant root and rhizome by water culture and cutting - Google Patents
Method for quick rooting of divaricate velvetplant root and rhizome by water culture and cutting Download PDFInfo
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- CN104381105A CN104381105A CN201410590348.0A CN201410590348A CN104381105A CN 104381105 A CN104381105 A CN 104381105A CN 201410590348 A CN201410590348 A CN 201410590348A CN 104381105 A CN104381105 A CN 104381105A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a method for quick rooting of divaricate velvetplant root and rhizome by water culture and cutting and belongs to the technical field of rooting of the divaricate velvetplant root and rhizome. The method mainly comprises a basic formula of quick rooting culture solution, preparation for the quick rooting culture solution and a method for quick rooting by the water culture and cutting. According to the method disclosed by the invention, with simplicity, practicality, economy and high efficiency as the rule, and with the principle that the rooting of plants can be promoted according to the mixture ratio of auxin with proper concentration and the growth of the plants can be promoted according to the mixture ratio of inorganic salts with proper concentration, the divaricate velvetplant root and rhizome is rooted by the water culture and the cutting through adopting a water culture mode of nutrient solution with cold boiled water (river water or tap water placed for over 1-2 days) and the like as mediums; meanwhile, the growth of green alga is suppressed by means of blacking a container and other paths, quick rooting of the divaricate velvetplant root and rhizome by the water culture and the cutting is realized, the rooting speed, root amount, survival rate, plant robustness and the like of the divaricate velvetplant root and rhizome by the water culture and the cutting are greatly improved. According to the method disclosed by the invention, the survival rate of the divaricate velvetplant root and rhizome by the water culture can reach over 98 percent, the divaricate velvetplant root and rhizome can be rooted in 6-7 days, the root amount is abundant, and cuttings are quick in growth.
Description
Technical field
The invention belongs to Radix et Rhizoma Gynurae divaricatae rooting technique field.
Background technology
Flower Plants in Hydroponic Culture is exactly take water as the cultivation that medium carries out, and for traditional earth culture, water planting has the features such as sight is strong, clean environment firendly, maintenance management are convenient, damage by disease and insect is few, therefore deeply likes by masses.Rooted Cuttings in the market has two kinds, and a kind of is real Rooted Cuttings; A kind of is the Rooted Cuttings earth culture seedling of taking root being rinsed well the vacation being used as water planting again, be called that soil washes seedling, because the earth culture root system of plant and water planting root system exist larger difference in structure and physiological property, soil is washed after seedling water planting usually because physiology is uncomfortable, cause rotten, be difficult to survive.
Radix et Rhizoma Gynurae divaricatae, another name white chessman grass, rich and honour dish, Gynura divaricata etc., for composite family gynura segetum belongs to perennial root herbaceous plant, containing nutritive element such as a large amount of phycocolloid element mannitol Cobastab phosphorus potassium and several amino acids etc. in its cauline leaf, thus there is effect that extremely strong reducing blood pressure and blood fat suppresses diabetes.Indoor placement can also play the effect of sterilizing and purifying air.In the later stage nineties 20th century, develop into artificial cultivation from naturally wild gradually, integrate medicinal, edible, sight, there is wide DEVELOPMENT PROSPECT.
Radix et Rhizoma Gynurae divaricatae fecundity is poor, seed amount is few, seedling is slow, production often adopts cottage propagation, can root induction determine the cuttage key technique that survive, and growth hormone kind and concentration and nutrition content are the key factors affecting water culture cuttage root-taking speed, the amount of taking root, root system and plant growth condition.Also can take root although Radix et Rhizoma Gynurae divaricatae branch is directly inserted in water, rooting rate is low, rooting rate is slow, root amount is few, and growing way is weak.Therefore, carry out the technical research of Radix et Rhizoma Gynurae divaricatae Water culture root induction, exploring can the culture fluid formula of Rapid Rooting and cottage method, and the Household water for Radix et Rhizoma Gynurae divaricatae is supported, water planting factorial seedling growth and produce significant.At present about the research of Radix et Rhizoma Gynurae divaricatae Water culture root induction technical elements has no report.
Summary of the invention
The object of this invention is to provide a kind of method of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting, to solve prior art Problems existing.
The technical solution adopted for the present invention to solve the technical problems is: the present invention with simple, practical, economical and efficient for principle, the principle of the mineral salt proportioning energy Promoting plant growth of plant establishment and debita spissitudo can be promoted according to debita spissitudo growth hormone proportioning, adopt the mode of suspension culture, with cold water (river or place the running water of more than 1-2 days) etc. for medium, add methyl α-naphthyl acetate (NAA) 0.05-0.2mg/L, indolebutyric acid (IBA) 0.1-1mg/L, potassium nitrate (KNO
3) 850-1000mg/L, ammonium nitrate (NH
4nO
3) 800-850 mg/L, potassium dihydrogen phosphate (KH
2pO
4) 80-95 mg/L, epsom salt (MgSO
47H
2o) 180-195mg/L, calcium chloride dihydrate (CaCl
22H
2o) 200-250mg/L, disodium ethylene diamine tetraacetate (Na
2-EDTA) 12-13mg/L, ferrous sulfate heptahydrate (FeSO
47H
2o) 18-19mg/L is made into nutrient solution, carry out Water culture to Radix et Rhizoma Gynurae divaricatae to take root, simultaneously by the approach such as container blacking being suppressed the growth of green alga, achieve the Rapid Rooting of Radix et Rhizoma Gynurae divaricatae Water culture, substantially increase speed, root amount, survival rate, robust plant degree etc. that Radix et Rhizoma Gynurae divaricatae Water culture is taken root.
A kind of basic recipe of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting culture fluid is as follows:
Containing potassium nitrate (KNO in every premium on currency
3) 850-1000mg, ammonium nitrate (NH
4nO
3) 800-850mg, potassium dihydrogen phosphate (KH
2pO
4) 80-95mg, epsom salt (MgSO
47H
2o) 180-195mg, calcium chloride dihydrate (CaCl
22H
2o) 200-250mg, disodium ethylene diamine tetraacetate (Na
2-EDTA) 12-13mg, ferrous sulfate heptahydrate (FeSO
47H
2o) 18-19mg, methyl α-naphthyl acetate (NAA) 0.05-0.2mg, indolebutyric acid (IBA) 0.1-1mg.
The preparation method of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting culture fluid:
The preparation of mother liquor A: potassium nitrate (KNO
3) 8.5-10g, ammonium nitrate (NH
4nO
3) 8-8.5g, potassium dihydrogen phosphate (KH
2pO
4) 0.8-0.95g, epsom salt (MgSO
47H
2o) 1.8-1.95g, calcium chloride dihydrate (CaCl
22H
2o) 2-2.5g, dissolve with distilled water respectively, join successively in volumetric flask in order, often add one, fully shake up, last constant volume is to 1000ml, and become 10 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of mother liquor B: Na
2-EDTA 0.6-0.65g, FeSO
47H
2o 0.9-0.95g, adding distil water mixes after dissolving respectively, stirs and heat 10-15min to make its abundant chelating, and then constant volume is to 500ml, and become 100 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of NAA: the balance with 1/10000 takes the NAA of 5-20mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of IBA: the balance with 1/10000 takes the IBA of 10-100mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of culture of rootage liquid: get mother liquor A:100ml, B:10ml, NAA:1ml, IBA:1ml, be diluted to 1000ml with cold water, adjusts PH to 5.8-6.2, is culture of rootage liquid.
A method for Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting, comprises the following steps:
A) preparation of cutting
The strain of Radix et Rhizoma Gynurae divaricatae good species is chosen the annotinous branch of robust growth without damage by disease and insect, is cut into the stem section of 10-15cm, stem
Duan Shangduan scabbles, and the shape of a hoof is splayed in lower end.The blade that 2-3 sheet is complete is stayed in the upper end of each stem section.
B) preparation of culture of rootage liquid
The preparation of mother liquor A: potassium nitrate (KNO
3) 8.5-10g, ammonium nitrate (NH
4nO
3) 8-8.5g, potassium dihydrogen phosphate (KH
2pO
4) 0.8-0.95g, epsom salt (MgSO
47H
2o) 1.8-1.95g, calcium chloride dihydrate (CaCl
22H
2o) 2-2.5g, dissolve with distilled water respectively, join successively in volumetric flask in order, often add one, fully shake up, last constant volume is to 1000ml, and become 10 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of mother liquor B: Na
2-EDTA 0.6-0.65g, FeSO
47H
2o 0.9-0.95g, adding distil water mixes after dissolving respectively, stirs and heat 10-15min to make its abundant chelating, and then constant volume is to 500ml, and become 100 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of NAA: the balance with 1/10000 takes the NAA of 5-20mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of IBA: the balance with 1/10000 takes the IBA of 10-100mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use.
The preparation of culture of rootage liquid: get mother liquor A:100ml, B:10ml, NAA:1ml, IBA:1ml, be diluted to 1000ml with cold water, adjusts PH to 5.8-6.2, is culture of rootage liquid.
C) culture vessel
The preparation of water planting bottle or basin: the apparatus useable glass bottle of Water culture or plastic basin, transparent vial or plastic basin paint painted black, and with the 84 medicining liquid dipping 25-40min of 0.15-0.3%, clear water is rinsed well, for subsequent use.
The making of cuttage plate, with the polystyrene foam plate that 1-2cm is thick, is cut into bottleneck size or is slightly less than basin mouth, and with card punch punching, a hole made a call to by the cystosepiment covered on bottle in centre, the cystosepiment on basin makes a call to a hole every 8-12cm, and aperture is 0.8-1cm.
D) Water culture: the nutrient solution culture of rootage liquid adding 2/3 volume in bottle or in basin, cutting is inserted in foam plate hole, and fix with sponge, cystosepiment is put on bottle or in basin, makes the stem section of cutting base portion 2-3cm touch nutrient solution.
E) condition of culture: temperature 18-25 DEG C, air humidity 60-70%, illumination 1000-2000lux, namely start after 6,7 days to take root, axillalry bud starts to sprout, and rooting rate reaches more than 98%.
The present invention is with simply, practical, economical and efficient is principle, the principle of the mineral salt proportioning energy Promoting plant growth of plant establishment and debita spissitudo can be promoted according to debita spissitudo growth hormone proportioning, adopt the mode of suspension culture, with cold water (river or place the running water of more than 1-2 days) etc. for medium, carry out Water culture to Radix et Rhizoma Gynurae divaricatae to take root, simultaneously by the approach such as container blacking being suppressed the growth of green alga, achieve the Rapid Rooting of Radix et Rhizoma Gynurae divaricatae Water culture, substantially increase the speed that Radix et Rhizoma Gynurae divaricatae Water culture is taken root, root amount, survival rate, robust plant degree etc.The present invention can make Radix et Rhizoma Gynurae divaricatae water planting survival rate can take root for more than 98%, 6-7 day, and root amount is enriched, and cutting growth rapidly.
Embodiment
The present invention with simple, practical, economical and efficient for principle, the principle of the mineral salt proportioning energy Promoting plant growth of plant establishment and debita spissitudo can be promoted according to debita spissitudo growth hormone proportioning, adopt the mode of suspension culture, be medium with cold water, add methyl α-naphthyl acetate (NAA) 0.1mg/L, indolebutyric acid (IBA) 0.5mg/L, potassium nitrate (KNO
3) 900mg/L, ammonium nitrate (NH
4nO
3) 820mg/L, potassium dihydrogen phosphate (KH
2pO
4) 85mg/L, epsom salt (MgSO
47H
2o) 185mg/L, calcium chloride dihydrate (CaCl
22H
2o) 220mg/L, disodium ethylene diamine tetraacetate (Na
2-EDTA) 12.8mg/L, ferrous sulfate heptahydrate (FeSO
47H
2o) 18.6mg/L is made into nutrient solution, carry out Water culture to Radix et Rhizoma Gynurae divaricatae to take root, simultaneously by the approach such as container blacking being suppressed the growth of green alga, achieve the Rapid Rooting of Radix et Rhizoma Gynurae divaricatae Water culture, substantially increase speed, root amount, survival rate, robust plant degree etc. that Radix et Rhizoma Gynurae divaricatae Water culture is taken root.
A kind of basic recipe of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting culture fluid is as follows:
Containing potassium nitrate (KNO in every premium on currency
3) 950, ammonium nitrate (NH
4nO
3) 820mg, potassium dihydrogen phosphate (KH
2pO
4) 85mg, epsom salt (MgSO
47H
2o) 185mg, calcium chloride dihydrate (CaCl
22H
2o) 220mg, disodium ethylene diamine tetraacetate (Na
2-EDTA) 12.8mg, ferrous sulfate heptahydrate (FeSO
47H
2o) 18.6mg, methyl α-naphthyl acetate (NAA) 0.1mg, indolebutyric acid (IBA) 0.5mg.
The preparation method of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting culture fluid:
The preparation of mother liquor A: potassium nitrate (KNO
3) 9.5g, ammonium nitrate (NH
4nO
3) 8.2g, potassium dihydrogen phosphate (KH
2pO
4) 0.85g, epsom salt (MgSO
47H
2o) 1.85g, calcium chloride dihydrate (CaCl
22H
2o) 2.2g, dissolve with distilled water respectively, join successively in volumetric flask in order, often add one, fully shake up, last constant volume is to 1000ml, and become 10 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of mother liquor B: Na
2-EDTA0.62g, FeSO
47H
2o 0.93g, adding distil water mixes after dissolving respectively, stirs and heat 12min to make its abundant chelating, and then constant volume is to 500ml, and become 100 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of NAA: the balance with 1/10000 takes the NAA of 15mg, after dissolving with the 1N NaOH of 3ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of IBA: the balance with 1/10000 takes the IBA of 80mg, after dissolving with the 1N NaOH of 3ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of culture of rootage liquid: get mother liquor A:100ml, B:10ml, NAA:1ml, IBA:1ml, be diluted to 1000ml with cold water, adjusts PH to 6, is culture of rootage liquid.
A method for Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting, comprises the following steps:
A) preparation of cutting
The strain of Radix et Rhizoma Gynurae divaricatae good species is chosen the annotinous branch of robust growth without damage by disease and insect, is cut into the stem section of 10-15cm, stem
Duan Shangduan scabbles, and the shape of a hoof is splayed in lower end.The blade that 2-3 sheet is complete is stayed in the upper end of each stem section.
B) preparation of culture of rootage liquid
The preparation of mother liquor A: potassium nitrate (KNO
3) 9.5g, ammonium nitrate (NH
4nO
3) 8.2g, potassium dihydrogen phosphate (KH
2pO
4) 0.85g, epsom salt (MgSO
47H
2o) 1.85g, calcium chloride dihydrate (CaCl
22H
2o) 2.2g, dissolve with distilled water respectively, join successively in volumetric flask in order, often add one, fully shake up, last constant volume is to 1000ml, and become 10 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of mother liquor B: Na
2-EDTA0.62g, FeSO
47H
2o 0.93g, adding distil water mixes after dissolving respectively, stirs and heat 12min to make its abundant chelating, and then constant volume is to 500ml, and become 100 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of NAA: the balance with 1/10000 takes the NAA of 15mg, after dissolving with the 1N NaOH of 3ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of IBA: the balance with 1/10000 takes the IBA of 80mg, after dissolving with the 1N NaOH of 3ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 2 DEG C of Refrigerator stores are for subsequent use.
The preparation of culture of rootage liquid: get mother liquor A:100ml, B:10ml, NAA:1ml, IBA:1ml, be diluted to 1000ml with cold water, adjusts PH to 6, is culture of rootage liquid.
C) culture vessel
The preparation of water planting basin: the apparatus plastic basin of Water culture, with the 84 medicining liquid dipping 30min of 0.2%, clear water is rinsed well, for subsequent use.
The making of cuttage plate, with the polystyrene foam plate that 2cm is thick, is cut into and is less than basin mouth, and with card punch punching, the cystosepiment on basin makes a call to a hole every 10cm, and aperture is 0.8cm.
D) Water culture: the nutrient solution culture of rootage liquid adding 2/3 volume in bottle or in basin, cutting is inserted in foam plate hole, and fix with sponge, cystosepiment is put on bottle or in basin, makes the stem section of cutting base portion 3cm touch nutrient solution.
E) condition of culture: temperature 20 DEG C, air humidity 60%, illumination 1500lux, namely start after 7 days to take root, axillalry bud starts to sprout, and rooting rate reaches more than 98%.
Claims (3)
1. a method for Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting, is characterized in that: the basic recipe of culture fluid comprises:
Containing potassium nitrate (KNO in every premium on currency
3) 850-1000mg, ammonium nitrate (NH
4nO
3) 800-850mg, potassium dihydrogen phosphate (KH
2pO
4) 80-95mg, epsom salt (MgSO
47H
2o) 180-195mg, calcium chloride dihydrate (CaCl
22H
2o) 200-250mg, disodium ethylene diamine tetraacetate (Na
2-EDTA) 12-13mg, ferrous sulfate heptahydrate (FeSO
47H
2o) 18-19mg, methyl α-naphthyl acetate (NAA) 0.05-0.2mg, indolebutyric acid (IBA) 0.1-1mg.
2. a preparation method for the culture fluid described in method of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting, is characterized in that: the preparation method of Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting culture fluid comprises:
1) preparation of mother liquor A: potassium nitrate (KNO
3) 8.5-10g, ammonium nitrate (NH
4nO
3) 8-8.5g, potassium dihydrogen phosphate (KH
2pO
4) 0.8-0.95g, epsom salt (MgSO
47H
2o) 1.8-1.95g, calcium chloride dihydrate (CaCl
22H
2o) 2-2.5g, dissolve with distilled water respectively, join successively in volumetric flask in order, often add one, fully shake up, last constant volume is to 1000ml, and become 10 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
2) preparation of mother liquor B: Na
2-EDTA 0.6-0.65g, FeSO
47H
2o 0.9-0.95g, adding distil water mixes after dissolving respectively, stirs and heat 10-15min to make its abundant chelating, and then constant volume is to 500ml, and become 100 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
3) preparation of NAA: the balance with 1/10000 takes the NAA of 5-20mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
4) preparation of IBA: the balance with 1/10000 takes the IBA of 10-100mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
5) preparation of culture of rootage liquid: get mother liquor A:100ml, B:10ml, NAA:1ml, IBA:1ml, be diluted to 1000ml with cold water, adjusts PH to 5.8-6.2, is culture of rootage liquid.
3. a method for Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting, its feature: the method for Radix et Rhizoma Gynurae divaricatae Water culture Rapid Rooting comprises the following steps:
A) preparation of cutting
The strain of Radix et Rhizoma Gynurae divaricatae good species is chosen the annotinous branch of robust growth without damage by disease and insect, is cut into the stem section of 10-15cm, stem
Duan Shangduan scabbles, and the shape of a hoof is splayed in lower end; The blade that 2-3 sheet is complete is stayed in the upper end of each stem section;
B) preparation of culture of rootage liquid
The preparation of mother liquor A: potassium nitrate (KNO
3) 8.5-10g, ammonium nitrate (NH
4nO
3) 8-8.5g, potassium dihydrogen phosphate (KH
2pO
4) 0.8-0.95g, epsom salt (MgSO
47H
2o) 1.8-1.95g, calcium chloride dihydrate (CaCl
22H
2o) 2-2.5g, dissolve with distilled water respectively, join successively in volumetric flask in order, often add one, fully shake up, last constant volume is to 1000ml, and become 10 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
The preparation of mother liquor B: Na
2-EDTA 0.6-0.65g, FeSO
47H
2o 0.9-0.95g, adding distil water mixes after dissolving respectively, stirs and heat 10-15min to make its abundant chelating, and then constant volume is to 500ml, and become 100 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
The preparation of NAA: the balance with 1/10000 takes the NAA of 5-20mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
The preparation of IBA: the balance with 1/10000 takes the IBA of 10-100mg, after dissolving with the 1N NaOH of 1-5ml, adding distil water constant volume is to 100ml, and become 1000 times of liquid, 0-4 DEG C of Refrigerator store is for subsequent use;
The preparation of culture of rootage liquid: get mother liquor A:100ml, B:10ml, NAA:1ml, IBA:1ml, be diluted to 1000ml with cold water, adjusts PH to 5.8-6.2, is culture of rootage liquid;
C) culture vessel
The preparation of water planting bottle or basin: the apparatus useable glass bottle of Water culture or plastic basin, transparent vial or plastic basin paint painted black, and with the 84 medicining liquid dipping 25-40min of 0.15-0.3%, clear water is rinsed well, for subsequent use;
The making of cuttage plate, with the polystyrene foam plate that 1-2cm is thick, is cut into bottleneck size or is less than basin mouth, and with card punch punching, a hole made a call to by the cystosepiment covered on bottle in centre, the cystosepiment on basin makes a call to a hole every 8-12cm, and aperture is 0.8-1cm;
D) Water culture: the nutrient solution culture of rootage liquid adding 2/3 volume in bottle or in basin, cutting is inserted in foam plate hole, and fix with sponge, cystosepiment is put on bottle or in basin, makes the stem section of cutting base portion 2-3cm touch nutrient solution;
E) condition of culture: temperature 18-25 DEG C, air humidity 60-70%, illumination 1000-2000lux, namely start after 6,7 days to take root, axillalry bud starts to sprout, and rooting rate reaches more than 98%.
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| CN105746322A (en) * | 2016-03-29 | 2016-07-13 | 河南省农业科学院经济作物研究所 | Method for quickly conserving and reproducing cotton by utilizing cotton leaves |
| CN105815196A (en) * | 2016-03-29 | 2016-08-03 | 河南省农业科学院经济作物研究所 | Method for rapid conservation propagation of black sesame by using black sesame leaves |
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| CN107439349A (en) * | 2017-08-21 | 2017-12-08 | 四川格睿园林科技有限公司 | A kind of Water culture propagation method of red maple |
| CN109769862A (en) * | 2019-03-25 | 2019-05-21 | 湖南省林大油茶有限公司 | A kind of oil tea cuttage root-taking agent |
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