CN105746322A - Method for quickly conserving and reproducing cotton by utilizing cotton leaves - Google Patents

Method for quickly conserving and reproducing cotton by utilizing cotton leaves Download PDF

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Publication number
CN105746322A
CN105746322A CN201610184775.8A CN201610184775A CN105746322A CN 105746322 A CN105746322 A CN 105746322A CN 201610184775 A CN201610184775 A CN 201610184775A CN 105746322 A CN105746322 A CN 105746322A
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blade
cotton
content
water planting
root
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CN105746322B (en
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房卫平
赵付安
李毅
谢德意
李武
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Henan Academy of Agricultural Sciences
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Henan Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

Abstract

The invention provides a method for quickly conserving and reproducing cotton by utilizing cotton leaves. The method comprises the steps of a, cutting leaves with axillary buds from a cotton plant; b, properly trimming the petioles of the cut leaves with the axillary buds; c, performing water culture on the trimmed leaves until the petioles are rooted; and d, performing soil culture on the rooted leaves. According to the method, the conservation and reproduction are carried out by utilizing the cotton leaves with the axillary buds, the cotton leaves with the axillary buds are subjected to water culture until the petioles are rooted, rooted leaf explants are planted in soil, and the axillary buds form a complete plant; the method has the advantages of high survival rate, low external environment requirement, low cost, short period, high simplicity, high reliability and the like, thereby being suitable for industrialization.

Description

The method utilizing the quick child care breeding Cotton Gossypii of cotton leaf
Technical field:
The invention belongs to the rapid propagation and culture technical field of plant, be specifically related to a kind of method utilizing the quick child care breeding Cotton Gossypii of cotton leaf.
Background technology:
Cotton Gossypii is the industrial crops that China is critically important, and at present, the breeding of Cotton Gossypii is typically all employing sexual propagation, namely utilizes cotton seeds to breed.
For realizing the asexual Fast-propagation of Cotton Gossypii, plant cuttage technology is incorporated in the child care breeding of Cotton Gossypii by technical staff, and cuttage is one of vegetative conventional means of plant, wherein most commonly seen with axis branch cuttage.Would generally use plant growth regulator that axis branch is processed during cuttage, to accelerate to take root.
But, the fast numerous means material requested of existing Cotton Gossypii is relatively big, at least needs the stem branch of one section of Cotton Gossypii, damages bigger to cotton plant, it is necessary to stricter condition, survival rate is relatively low, and the cycle is longer, relatively costly.In order to adapt to the requirement of Cotton Gossypii simplifying cultivation technique, it is necessary to find more effective, easier, more economical method.
Summary of the invention:
In sum, in order to overcome the deficiency of prior art problem, the invention provides a kind of method utilizing the quick child care breeding Cotton Gossypii of cotton leaf, it is that the blade with axillalry bud utilizing Cotton Gossypii carries out child care breeding, cotton leaf water planting with axillalry bud being taken root to petiole, plant in soil by the leaf explant after taking root, axillalry bud forms whole plant, the method has survival rate height, not needing strict external environment condition, cost is low, and the cycle is short, simple and reliable, it is suitable for the advantages such as batch production.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of method utilizing the quick child care breeding Cotton Gossypii of cotton leaf, wherein: comprise the following steps that:
A, cutting the blade with axillalry bud on cotton plants, the blade cut is the blade being in functional period;
B, the petiole with the blade of axillalry bud cut suitably is pruned, when petiole is pruned, reject petiolar lignified tissue, be maintained with axillalry bud intact, and repeatedly rinse the blade after pruning with distilled water;
C, to prune after blade carry out water planting, take root to petiole, when blade is carried out water planting, petiole and axillalry bud are immersed in water planting liquid, and blade is placed on cystosepiment, and cystosepiment is suspended on water planting liquid level;
D, to carrying out earth culture after the blade taken root, earth culture is to be transplanted in earth culture substrate by the blade after taking root in step c so that it is growth naturally, the length of the root that blade bears is that at least 5cm just can carry out earth culture.
Technical scheme it may also is that realize: the described blade in step a can be cotton plant Leaves on main stem, side shoot blade or Fruit branch blade.
Technical scheme is it may also is that realize: rejects petiolar lignified tissue cut-out phloem tissue in described step b, is maintained with axillalry bud intact, repeatedly rinses blade after pruning at least three times with distilled water.
Technical scheme it may also is that realize: when blade is carried out water planting by described step c, making is of moderate size, open-topped container, water planting liquid at container content 4/5 volume, water planting liquid liquid level is placed one block of cystosepiment, the petiole of the blade after pruning and axillalry bud are immersed in water planting liquid, blade is placed on cystosepiment, cystosepiment is suspended on water planting liquid level, water planting temperature 23 ~ 33 DEG C during water planting, adopt sun exposure or adopt artificial daylight light irradiation, light application time 4 ~ 15 hour/day.
Technical scheme it may also is that realize: after taking root in described step d, and root length degree reaches the blade of at least more than 5cm, is transplanted in earth culture substrate, during transplanting, root is all embedded in earth culture substrate, and axillalry bud exposes stromal surface, make it naturally grow, carry out Routine Management.
Technical scheme is it may also is that realize: described water planting liquid is distilled water or for nutritional solution.
Technical scheme it may also is that realize: described nutritional solution includes four water calcium chloride, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, iron salt solutions and liquid microelement, and wherein the content of four water calcium chloride is 460~480mg/L;The content of potassium nitrate is 245~260mg/L;The content of ammonium nitrate is 30~50mg/L, and the content of potassium dihydrogen phosphate is 60~80mg/L;The content of magnesium sulfate is 240~255mg/L;Iron salt solutions adopts 1 times of iron salt solutions, and its content is 1~1.5ml/L;Liquid microelement adopts 1000 times of liquid microelements, and its content is 2~3ml/L.
Technical scheme it may also is that realize: described nutritional solution includes four water calcium chloride, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, iron salt solutions and liquid microelement, and wherein the content of four water calcium chloride is 472mg/L;The content of potassium nitrate is 253mg/L;The content of ammonium nitrate is 40mg/L, and the content of potassium dihydrogen phosphate is 68mg/L;The content of magnesium sulfate is 247mg/L;Iron salt solutions adopts 1 times of iron salt solutions, and its content is 1.3ml/L;Liquid microelement adopts 1000 times of liquid microelements, and its content is 2.5ml/L.
Technical scheme is it may also is that realize: described earth culture substrate is Cotton Gossypii land for growing field crops soil or formulated according to 1:1 mass ratio by Nutrition Soil and Vermiculitum.
The invention have the benefit that
1, the present invention is that the blade with axillalry bud utilizing Cotton Gossypii carries out child care breeding, cotton leaf water planting with axillalry bud is taken root to petiole, leaf explant after taking root is planted in soil, axillalry bud forms whole plant, and the method has survival rate height, does not need strict external environment condition, cost is low, cycle is short, simple and reliable, is suitable for the advantages such as batch production.
2, the present invention is easy to operate, flow process is simple, industrial massive reliable and stable, applicable, and the cycle is short, after water planting, within about 7 days, can take root.After earth culture, within one month, can buddingging, plant is precocious, and the survival rate of plant of formation is high.The present invention does not need strict external environment condition, the effect that all can reach under indoor conditions and under field condition.
3, the present invention adopts the novel modes of reproduction of earth culture after first water planting, effectively alleviates the inhibitory action that leaf explant is taken root by aldehydes matter, it is simple to the growth of cotton root system, it is simple to the root induction of natural cuttage during Cotton Gossypii earth culture.
4, the present invention preserves the field such as fixing with breeding, male sterility breeding and heterosis, hybrid vigor at Cotton Gossypii certain material and will have bigger using value, and the present invention is for lignifying, and the similar research of semi-lignified plant has some reference value.
Accompanying drawing illustrates:
Fig. 1 is the blade water planting view of the present invention;
Fig. 2 is ten days view of blade water planting of the present invention;
Fig. 3 is the blade earth culture view of the present invention;
Fig. 4 is 15 days view of blade earth culture of the present invention;
Fig. 5 is 30 days view of blade earth culture of the present invention;
Fig. 6 is the cotton plant plant comparison diagram that the cotton plants that the present invention cultivates is cultivated with cotton seeds.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment one
As shown in Fig. 1 ~ 6, the present embodiment, for Gossypium hirsutum L. Ji cotton 11, utilizes the blade Fast-propagation Cotton Gossypii of Gossypium hirsutum L. Ji cotton 11, comprises the following steps that:
A, Gossypium hirsutum L. Ji cotton 11 is normally planted in cotton field, Routine Management, then cut the blade with axillalry bud on cotton 11 plant in Gossypium hirsutum L. Ji, the blade cut is the blade being in functional period, and this blade can be cotton plant Leaves on main stem, side shoot blade or Fruit branch blade.
B, the petiole with the blade of axillalry bud cut suitably is pruned, reject petiolar lignified tissue cut-out phloem tissue, be maintained with axillalry bud intact, repeatedly rinse blade after pruning three times with distilled water.
C, the blade after pruning being carried out water planting, when blade is carried out water planting, petiole and axillalry bud are immersed in water planting liquid, and blade is placed on cystosepiment, and cystosepiment is suspended on water planting liquid level and takes root to petiole;
Making is of moderate size, open-topped container, at the distilled water of container content 4/5 volume, distilled water is water planting liquid, places one block of cystosepiment on distilled water liquid level, is immersed in distilled water by the petiole of the blade after pruning and axillalry bud, blade is placed on cystosepiment, cystosepiment is suspended on water planting liquid level, water planting temperature 23 ~ 33 DEG C during water planting, adopts sun exposure, light application time 6 ~ 14 hour/day, when petiole is taken root, and when root length degree reaches 5cm, prepare earth culture.
D, the blade taken root being carried out earth culture, earth culture is to be transplanted in earth culture substrate by the blade after taking root in step c so that it is growth naturally, and the length of the root that blade bears is that 5cm carries out earth culture:
After taking root, and root length degree reaches the blade of more than 5cm, being transplanted in earth culture substrate, earth culture substrate adopts Cotton Gossypii land for growing field crops soil, during transplanting, root is all embedded in earth culture substrate, and axillalry bud exposes stromal surface so that it is growth naturally, carry out Routine Management, grow up to after highly moderate cotton plant Seedling until axillalry bud, by cotton plant transplantation of seedlings to land for growing field crops, carry out the management of conventional cotton field.
Observe above-mentioned whole cultivating process to find:
1), during water planting, namely about 7 days petioles form obvious root, water planting about 10 days, and root length degree is most more than 5 centimetres, and axillalry bud there has also been a degree of growth simultaneously.
2) when water planting, temperature is 28 degree, is comparatively suitable for the growth of cotton root.
3), when earth culture, in 1 week, Cotton Gossypii axillary bud growth is slow, and after one week, axillary bud growth is rapid.After general 1 month, axillary bud development become cotton plants start alabastrum occurs.The plant being newly formed can normally budding, and blooms, result.
4) cotton plants formed by this kind of method, has plant type compact, loose, downgrades substantially, and growing way is more consistent, precocious feature.
5) plant formed by this kind of method, survival rate is close to 100%.
6) be can be seen that by Figure of description Fig. 6 of the present invention, the cotton plant that cotton seeds is cultivated just there will be alabastrum after needing to be long to certain altitude, it is about about the 10th true leaf of cotton plant, and the cotton plants that the present invention cultivates just may occur in which Cotton Gossypii alabastrum at the 3rd or the 4th true leaf place.
The present embodiment is it is shown that the present invention can utilize cotton No. 11 of blade Fast-propagation child care Gossypium hirsutum L. Ji.
Embodiment two
The present embodiment, for sea island cotton sea 7124, utilizes sea island cotton sea 7124 blade Fast-propagation Cotton Gossypiis, comprises the following steps that:
A, sea island cotton sea 7124 is normally planted in cotton field, Routine Management, then the blade with axillalry bud on 7124 plant of sea island cotton sea is cut, the blade cut is the blade being in functional period, this blade can be cotton plant Leaves on main stem, side shoot blade or Fruit branch blade, and must assure that axillalry bud is complete.
B, the petiole with the blade of axillalry bud cut suitably is pruned, reject petiolar lignified tissue cut-out phloem tissue, be maintained with axillalry bud intact, repeatedly rinse blade after pruning three times with distilled water.
C, to prune after blade carry out water planting, take root to petiole,
Making is of moderate size, open-topped container, at the nutritional solution of container content 4/5 volume, nutritional solution is water planting liquid, places one block of cystosepiment on the liquid level of nutritional solution, is fully immersed in nutritional solution by the petiole of the blade after pruning and axillalry bud, blade is placed on cystosepiment, cystosepiment is suspended on the liquid level of nutritional solution, water planting temperature 23 ~ 33 DEG C during water planting, adopts artificial daylight light irradiation, light application time 6 ~ 14 hour/day, when petiole is taken root, and root length degree at least up to 5cm time, prepare earth culture.Described nutritional solution includes four water calcium chloride, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, iron salt solutions and liquid microelement, and wherein the content of four water calcium chloride is 472mg/L;The content of potassium nitrate is 253mg/L;The content of ammonium nitrate is 40mg/L, and the content of potassium dihydrogen phosphate is 68mg/L;The content of magnesium sulfate is 247mg/L;Iron salt solutions adopts 1 times of iron salt solutions, and its content is 1.3ml/L;Liquid microelement adopts 1000 times of liquid microelements, and its content is 2.5ml/L.
D, the blade taken root being carried out earth culture, earth culture is to be transplanted in earth culture substrate by the blade after taking root in step c so that it is growth naturally, and the length of the root that blade bears is that 5cm carries out earth culture:
After taking root, and root length degree reaches the blade of more than 5cm, is transplanted in earth culture substrate, and earth culture substrate is that Nutrition Soil mixes according to 1:1 mass ratio with Vermiculitum, during transplanting, root is all embedded in earth culture substrate, and axillalry bud exposes stromal surface, tap water waters so that it is growth naturally, carries out Routine Management;Grow up to after highly moderate cotton plant Seedling until axillalry bud, by cotton plant transplantation of seedlings to land for growing field crops, carry out the management of conventional cotton field.
Observe above-mentioned whole cultivating process to find:
1), during water planting, namely about 7 days petioles form obvious root, water planting about 10 days, and root length degree is most more than 5 centimetres, and axillalry bud there has also been a degree of growth simultaneously.
2) when water planting, temperature is 28 degree, is comparatively suitable for the growth of cotton root.
3), when earth culture, in 1 week, Cotton Gossypii axillary bud growth is slow, and after one week, axillary bud growth is rapid.After general 1 month, axillary bud development become cotton plants start alabastrum occurs.The plant being newly formed can normally budding, and blooms, result.
4) cotton plants formed by this kind of method, has plant type compact, loose, downgrades substantially, and growing way is more consistent, precocious feature.
5) plant formed by this kind of method, survival rate is close to 100%.
The present embodiment is it is shown that the present invention can utilize blade Fast-propagation child care sea island cotton sea 7124.
Embodiment three
The present embodiment, for Asia cotton rock carefully sub-No. 1, utilizes Asia cotton rock carefully sub-No. 1 blade Fast-propagation Cotton Gossypii, comprises the following steps that:
A, by Asia cotton rock carefully sub-No. 1 normal plant in cotton field, Routine Management, then the blade with axillalry bud on Asia cotton rock carefully sub-No. 1 cotton plants is cut, the blade cut is the blade being in functional period, this blade can be cotton plant Leaves on main stem, side shoot blade or Fruit branch blade, and must assure that axillalry bud is complete.
B, the petiole with the blade of axillalry bud cut suitably is pruned, reject petiolar lignified tissue cut-out phloem tissue, be maintained with axillalry bud intact, repeatedly rinse blade after pruning three times with distilled water.
C, to prune after blade carry out water planting, take root to petiole,
Making is of moderate size, open-topped container, at the distilled water of container content 4/5 volume, distilled water is water planting liquid, places one block of cystosepiment on the liquid level of distilled water, is fully immersed in distilled water by the petiole of the blade after pruning and axillalry bud, blade is placed on cystosepiment, cystosepiment is suspended on the liquid level of distilled water, water planting temperature 23 ~ 33 DEG C during water planting, adopts artificial daylight light irradiation, light application time 8 ~ 14 hour/day, when petiole is taken root, and root length degree at least up to 5cm time, prepare earth culture.
D, the blade taken root being carried out earth culture, earth culture is to be transplanted in earth culture substrate by the blade after taking root in step c so that it is growth naturally, and the length of the root that blade bears is that 5cm carries out earth culture:
After taking root, and root length degree reaches the blade of more than 5cm, is transplanted in earth culture substrate, and earth culture substrate is that Nutrition Soil mixes according to 1:1 mass ratio with Vermiculitum, during transplanting, root is all embedded in earth culture substrate, and axillalry bud exposes stromal surface, tap water waters so that it is growth naturally, carries out Routine Management;Grow up to after highly moderate cotton plant Seedling until axillalry bud, by cotton plant transplantation of seedlings to land for growing field crops, carry out the management of conventional cotton field.
Observe above-mentioned whole cultivating process to find:
1), during water planting, namely about 7 days petioles form obvious root, water planting about 10 days, and root length degree is most more than 5 centimetres, and axillalry bud there has also been a degree of growth simultaneously.
2) when water planting, temperature is 30 degree, is comparatively suitable for the growth of cotton root.
3), when earth culture, in 1 week, Cotton Gossypii axillary bud growth is slow, and after one week, axillary bud growth is rapid.After general 1 month, axillary bud development become cotton plants start alabastrum occurs.The plant being newly formed can normally budding, and blooms, result.
4) cotton plants formed by this kind of method, has plant type compact, loose, downgrades substantially, and growing way is more consistent, precocious feature.
5) plant formed by this kind of method, survival rate is close to 100%.
The present embodiment is it is shown that the present invention can utilize blade Fast-propagation child care Asia cotton rock carefully sub-No. 1.
Embodiment four
The present embodiment is cotton for wild cotton Lei Mengdeshi, utilizes wild cotton Lei Mengdeshi cotton blade Fast-propagation Cotton Gossypii, comprises the following steps that:
A, wild cotton Lei Mengdeshi cotton is normally planted in cotton field, Routine Management, then the blade with axillalry bud on Asia cotton rock carefully sub-No. 1 cotton plants is cut, the blade cut is the blade being in functional period, this blade can be cotton plant Leaves on main stem, side shoot blade or Fruit branch blade, and must assure that axillalry bud is complete.
B, the petiole with the blade of axillalry bud cut suitably is pruned, reject petiolar lignified tissue cut-out phloem tissue, be maintained with axillalry bud intact, repeatedly rinse blade after pruning three times with distilled water.
C, to prune after blade carry out water planting, take root to petiole,
Making is of moderate size, open-topped container, at the distilled water of container content 4/5 volume, distilled water is water planting liquid, places one block of cystosepiment on the liquid level of distilled water, is fully immersed in distilled water by the petiole of the blade after pruning and axillalry bud, blade is placed on cystosepiment, cystosepiment is suspended on the liquid level of distilled water, water planting temperature 23 ~ 33 DEG C during water planting, adopts artificial daylight light irradiation, light application time 8 ~ 14 hour/day, when petiole is taken root, and root length degree at least up to 5cm time, prepare earth culture.
D, the blade taken root being carried out earth culture, earth culture is to be transplanted in earth culture substrate by the blade after taking root in step c so that it is growth naturally, and the length of the root that blade bears is that 5cm carries out earth culture:
After taking root, and root length degree reaches the blade of more than 5cm, is transplanted in earth culture substrate, and earth culture substrate is land for growing field crops soil, and during transplanting, root is all embedded in earth culture substrate, and axillalry bud exposes stromal surface, and tap water waters so that it is growth naturally, carries out Routine Management.Grow up to after highly moderate cotton plant Seedling until axillalry bud, by cotton plant transplantation of seedlings to land for growing field crops, carry out the management of conventional cotton field.
Observe above-mentioned whole cultivating process to find:
1), during water planting, namely about 7 days petioles form obvious root, water planting about 10 days, and root length degree is most more than 5 centimetres, and axillalry bud there has also been a degree of growth simultaneously.
2) when water planting, temperature is 30 degree, is comparatively suitable for the growth of cotton root.
3), when earth culture, in 1 week, Cotton Gossypii axillary bud growth is slow, and after one week, axillary bud growth is rapid.After general 1 month, axillary bud development become cotton plants start alabastrum occurs.The plant being newly formed can normally budding, and blooms, result.
4) cotton plants formed by this kind of method, has plant type compact, loose, downgrades substantially, and growing way is more consistent, precocious feature.
5) plant formed by this kind of method, survival rate is close to 100%.
The present embodiment is it is shown that the present invention can utilize blade Fast-propagation child care wild cotton Lei Mengdeshi cotton.
To illustrate that, above-described embodiment is illustrative not limiting to technical solution of the present invention, the equivalent replacement of art those of ordinary skill or other amendment made according to prior art, as long as not beyond the thinking of technical solution of the present invention and scope, should be included within interest field of the presently claimed invention.

Claims (9)

1. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf, it is characterised in that: comprise the following steps that:
A, cutting the blade with axillalry bud on cotton plants, the blade cut is the blade being in functional period;
B, the petiole with the blade of axillalry bud cut suitably is pruned, when petiole is pruned, reject petiolar lignified tissue, be maintained with axillalry bud intact, and repeatedly rinse the blade after pruning with distilled water;
C, to prune after blade carry out water planting, take root to petiole, when blade is carried out water planting, petiole and axillalry bud are immersed in water planting liquid, and blade is placed on cystosepiment, and cystosepiment is suspended on water planting liquid level;
D, to carrying out earth culture after the blade taken root, earth culture is to be transplanted in earth culture substrate by the blade after taking root in step c so that it is growth naturally, the length of the root that blade bears is that at least 5cm just can carry out earth culture.
2. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 1, it is characterised in that: the described blade in step a can be cotton plant Leaves on main stem, side shoot blade or Fruit branch blade.
3. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 1, it is characterized in that: described step b rejects petiolar lignified tissue, and cut-out phloem tissue, it is maintained with axillalry bud intact, repeatedly rinses blade after pruning at least three times with distilled water.
4. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 1, it is characterized in that: when blade is carried out water planting by described step c, making is of moderate size, open-topped container, water planting liquid at container content 4/5 volume, water planting liquid liquid level is placed one block of cystosepiment, the petiole of the blade after pruning and axillalry bud are immersed in water planting liquid, blade is placed on cystosepiment, cystosepiment is suspended on water planting liquid liquid level, water planting temperature 23 ~ 33 DEG C during water planting, adopt sun exposure or adopt artificial daylight light irradiation, light application time 4 ~ 15 hour/day.
5. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 1, it is characterized in that: in described step d, by after taking root and root length degree reach the blade of at least more than 5cm, it is transplanted in earth culture substrate, during transplanting, root is all embedded in earth culture substrate, and axillalry bud exposes earth culture stromal surface, make it naturally grow, carry out Routine Management.
6. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 4, it is characterised in that: described water planting liquid is distilled water or is nutritional solution.
7. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 6, it is characterized in that: described nutritional solution includes four water calcium chloride, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, iron salt solutions and liquid microelement, wherein the content of four water calcium chloride is 460~480mg/L;The content of potassium nitrate is 245~260mg/L;The content of ammonium nitrate is 30~50mg/L, and the content of potassium dihydrogen phosphate is 60~80mg/L;The content of magnesium sulfate is 240~255mg/L;Iron salt solutions adopts 1 times of iron salt solutions, and its content is 1~1.5ml/L;Liquid microelement adopts 1000 times of liquid microelements, and its content is 2~3ml/L.
8. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 6, it is characterized in that: described nutritional solution includes four water calcium chloride, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, iron salt solutions and liquid microelement, wherein the content of four water calcium chloride is 472mg/L;The content of potassium nitrate is 253mg/L;The content of ammonium nitrate is 40mg/L, and the content of potassium dihydrogen phosphate is 68mg/L;The content of magnesium sulfate is 247mg/L;Iron salt solutions adopts 1 times of iron salt solutions, and its content is 1.3ml/L;Liquid microelement adopts 1000 times of liquid microelements, and its content is 2.5ml/L.
9. the method utilizing the quick child care breeding Cotton Gossypii of cotton leaf according to claim 5, it is characterised in that: described earth culture substrate is Cotton Gossypii land for growing field crops soil or formulated according to 1:1 mass ratio by Nutrition Soil and Vermiculitum.
CN201610184775.8A 2016-03-29 2016-03-29 Utilize the method for the quick child care breeding cotton of cotton leaf Expired - Fee Related CN105746322B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112400584A (en) * 2019-08-19 2021-02-26 陈芳淼 Asexual rapid propagation method for dicotyledons

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