CN105104145A - Wax apple hydroponic cutting propagation method - Google Patents
Wax apple hydroponic cutting propagation method Download PDFInfo
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- CN105104145A CN105104145A CN201510549826.8A CN201510549826A CN105104145A CN 105104145 A CN105104145 A CN 105104145A CN 201510549826 A CN201510549826 A CN 201510549826A CN 105104145 A CN105104145 A CN 105104145A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a wax apple hydroponic cutting propagation method. The method includes the procedures of collection and management of cutting slips, preparation of a nutrient solution, preparation of a cuttage container, hydroponic culture, management performed after cuttage and acclimatization and transplantation, half-lignified wax apple tender twigs are selected and pruned to be inserted in the cuttage container containing the nutrient solution so that hydroponic culture can be performed, culture conditions are controlled, acclimatization and transplantation are performed after the cutting slips growing into young roots are obtained, and wax apple seedlings are obtained. By means of the method, female parents can be accumulated, and therefore large-scale propagation can be performed, meanwhile, the problem that the cuttage season is limited by time is solved, cutting propagation can be performed all the year around, the culture period is short, root growth is fast, environmental controllability is good, culture conditions are stable, the survival rate of the cutting slips is 95% or higher, and the method is applicable to large-scale production, can be popularized widely, and has good economic benefits, social benefits and ecological benefits.
Description
Technical field
The invention belongs to plant cuttage propagation field, relate to the cottage propagation of wax-apple, be specifically related to a kind of wax-apple Water culture propagation method.
Background technology
Wax-apple (EugeniajavanicaLam), has another name called a day peach, water Portugal or wax jambo, is the evergreen dungarunga of Myrtaceae.Originate in the Malay Peninsula, generally cultivate at Malaysia, Indonesia, Philippine and TaiWan, China, the wax-apple of TaiWan, China introduces TaiWan, China by Dutchman at the 17th century, and TaiWan, China Pingdong is the most famous place of production, is that a kind of primary growth is in the fruit in the torrid zone.
Wax-apple can use the method breedings such as sowing, cuttage, high pressure and grafting, and improved seeds often adopt the method such as grafting, cuttage to carry out vegetative propagation.During cottage propagation, general selection 5 years above wax-apples of life become fruit tree in age, and adopt green branch, semi-lignified branch and annual ripe branch cutting, rooting rate can reach 60-80%.During propagation by grafiting, the excellent maternal plant that output is high, fruit quality is good is picked up from scion more, and stock can use wax-apple seedling and congener Portugal, Wu Mo etc., and the conventional method such as cut-grafting, side grafting, graft survival rate is generally about 50-80%.Using wax-apple seedling as stock, compatibility is good, and graft survival rate is higher.But wax-apple cultivated population is little, and seed is few, stock output is lower, is difficult to meet production requirement.
Summary of the invention
The present invention, in order to overcome the deficiencies in the prior art, provides a kind of cultivation period short, expands numerous fast, environment controllability is good, condition of culture is stablized, survival rate is high wax-apple Water culture propagation method.
In order to realize foregoing invention object, the technical solution used in the present invention is as follows:
A kind of wax-apple Water culture propagation method, comprise cuttings sampling and processing, the preparation of nutrient solution, the preparation of cuttage vessel, water planting cultivated, the operation of management and acclimatization and transplants after cuttage, the raw then semi-lignified that growth selection is healthy and strong, bud is good, disease-free or lignified wax-apple young sprout, insert to be equipped with in the cuttage vessel of nutrient solution after pruning modes and carry out water planting cultivation, control condition of culture, carry out acclimatization and transplants after obtaining growing up to the cuttings of young root, obtain wax-apple nursery stock; Its concrete operation step is as follows:
(1) cuttings sampling and processing: the spray selecting wax-apple, intercepts the branch of semi-lignified part, prunes growth 15-20cm as cuttings, leaves and takes cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane; Before cuttage, with the water rinse cuttings 0.5h of cleaning, take out after draining away the water, quickly dipped 5s in ABT solution that basic for cuttings 3-5cm length is submerged;
(2) preparation of nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.4-1.5mg/L+GGR1.0-2.0mg/L+PG30-50mg/L+ sucrose 10g/L+ agar 2.0-5.0g/L+ active carbon 1.0-2.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.5-6.0;
(3) preparation of cuttage vessel: the casing selecting glass to make in cuttage indoor is as cuttage vessel, and pour nutrient solution in cuttage vessel, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and punch, as cuttings fixed head at cavities place card punch;
(4) water planting is cultivated: cuttings step (1) obtained is inserted in the aperture of cuttings fixed head, and makes cuttings inclination 30-45 °, is then placed in cuttage vessel;
(5) manage after cuttage: room temperature controls as 20-30 DEG C, indoor humidity controls as 80-100%, and solution temperature controls as 15-20 DEG C, and nutrient solution is changed in timing; With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat;
(6) acclimatization and transplants: when the cuttings young root of water planting grow to 1-2cm long time, implanting in seedling medium and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains wax-apple nursery stock.
Preferably, its content of ABT solution described in above step (1) is 1500mg/L.
Preferably, in above step (5), the frequency of oxygenation is every 7h ventilation 1h; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time when liquid level declines with distilled water.
Preferably, the wide 20-30cm of casing described in above step (3), long 50-60cm, dark 20-25cm; Aperture 1.0cm on described cuttings fixed head.
Preferably, the seedling medium described in above step (6) by coconut palm chaff and peat soil by volume for 1:1 Homogeneous phase mixing forms.
Relative to prior art, the advantage that the present invention has and beneficial effect as follows:
1, the present invention prepares excellent nutrient solution, have adjusted the pH value benefiting wax-apple and take root, and by the impact on wax-apple cuttage root-taking such as nutritive element, plant growth regulator, improves cutting plantation; Meanwhile, in cottage propagation process, the condition such as temperature, humidity, ventilation is completely controlled, and nutrient component absorbing capacity is strong, and well developed root system, cutting plantation is high and stable.
2, the present invention utilizes homemade casing to carry out Water culture cultivation as cuttage vessel; female parent can be accumulated; thus it is numerous to carry out a large amount of expansion; overcome the problem that cuttage is subject to time restriction season simultaneously; all can carry out cottage propagation throughout the year, cuttings survival rate more than 95%, is applicable to large-scale production; can widely popularize, there are good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
A kind of wax-apple Water culture propagation method, selects the spray of wax-apple, intercepts the branch of semi-lignified part, prune growth 15-20cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.4mg/L+GGR1.0mg/L+PG30mg/L+ sucrose 10g/L+ agar 2.0g/L+ active carbon 1.0g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.5.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 20cm of cuttage casing, long 50cm, dark 20cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3 ~ 5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 3-5cm length submerged is quickly dipped 5s in the solution of ABT1500mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 30 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, room temperature controls as 20-25 DEG C, and indoor humidity controls as 80-90%, and solution temperature controls as 15-18 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implant by coconut palm chaff and peat soil by volume for 1:1 Homogeneous phase mixing forms in seedling medium and be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains wax-apple nursery stock.
Embodiment 2:
A kind of wax-apple Water culture propagation method, selects the spray of wax-apple, intercepts the branch of semi-lignified part, prune growth 15-18cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.8mg/L+GGR1.5mg/L+PG40mg/L+ sucrose 10g/L+ agar 3.0g/L+ active carbon 2.0g/L, adds citric acid solution and nutrient solution pH value is adjusted to 6.0.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 25cm of cuttage casing, long 55cm, dark 20cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 3-5cm length submerged is quickly dipped 10s in the mixed solution of ABT250mg/L and indolebutyric acid 750mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 30 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, room temperature controls as 20-25 DEG C, and indoor humidity controls as 80-90%, and solution temperature controls as 15-18 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implant by coconut palm chaff and peat soil by volume for being colonizated in furrow in the seedling medium of 1:1 Homogeneous phase mixing, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains wax-apple nursery stock.
Embodiment 3:
A kind of wax-apple Water culture propagation method, selects the spray of wax-apple, intercepts the branch of semi-lignified part, prune growth 18-20cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO1.2mg/L+GGR2.0mg/L+PG40mg/L+ sucrose 10g/L+ agar 4.0g/L+ active carbon 1.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.9.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 30cm of cuttage casing, long 60cm, dark 25cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 3-5cm length submerged is quickly dipped 5s in the solution of ABT1500mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 45 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, it is 25 ~ 30 DEG C that room temperature controls, and it is 90 ~ 100% that indoor humidity controls, and solution temperature controls as 18-20 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implant by coconut palm chaff and peat soil by volume for being colonizated in furrow in the seedling medium of 1:1 Homogeneous phase mixing, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains wax-apple nursery stock.
Embodiment 4:
A kind of wax-apple Water culture propagation method, selects the spray of wax-apple, intercepts the branch of semi-lignified part, prune growth 15-20cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO1.5mg/L+GGR2.0mg/L+PG50mg/L+ sucrose 10g/L+ agar 5.0g/L+ active carbon 2.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 6.0.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 30cm of cuttage casing, long 60cm, dark 25cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 3-5cm length submerged is quickly dipped 5s in the solution of ABT1500mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 45 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, room temperature controls as 25-30 DEG C, and indoor humidity controls as 90-100%, and solution temperature controls as 15-20 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implant by coconut palm chaff and peat soil by volume for being colonizated in furrow in the seedling medium of 1:1 Homogeneous phase mixing, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains wax-apple nursery stock.
Claims (5)
1. a wax-apple Water culture propagation method, it is characterized in that: comprise cuttings sampling and processing, the preparation of nutrient solution, the preparation of cuttage vessel, water planting are cultivated, the operation of management and acclimatization and transplants after cuttage, select the wax-apple spray of semi-lignified, insert to be equipped with in the cuttage vessel of nutrient solution after pruning modes and carry out water planting cultivation, control condition of culture, carry out acclimatization and transplants after obtaining growing up to the cuttings of young root, obtain wax-apple nursery stock; Its concrete operation step is as follows:
(1) cuttings sampling and processing: the spray selecting wax-apple, intercepts the branch of semi-lignified part, prunes growth 15-20cm as cuttings, leaves and takes cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane; Before cuttage, with the water rinse cuttings 0.5h of cleaning, take out after draining away the water, quickly dipped 5s in ABT solution that basic for cuttings 3-5cm length is submerged;
(2) preparation of nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.4-1.5mg/L+GGR1.0-2.0mg/L+PG30-50mg/L+ sucrose 10g/L+ agar 2.0-5.0g/L+ active carbon 1.0-2.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.5-6.0;
(3) preparation of cuttage vessel: the casing selecting glass to make in cuttage indoor is as cuttage vessel, and pour nutrient solution in cuttage vessel, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and punch, as cuttings fixed head at cavities place card punch;
(4) water planting is cultivated: cuttings step (1) obtained is inserted in the aperture of cuttings fixed head, and makes cuttings inclination 30-45 °, is then placed in cuttage vessel;
(5) manage after cuttage: it is 20 ~ 30 DEG C that room temperature controls, it is 80 ~ 100% that indoor humidity controls, and solution temperature controls as 15-20 DEG C, and nutrient solution is changed in timing; With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat;
(6) acclimatization and transplants: when the cuttings young root of water planting grow to 1-2cm long time, implanting in seedling medium and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains wax-apple nursery stock.
2. wax-apple Water culture propagation method according to claim 1, is characterized in that: in step (1), described its content of ABT solution is 1500mg/L.
3. wax-apple Water culture propagation method according to claim 1, is characterized in that: in step (5), the frequency of oxygenation is every 7h ventilation 1h; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time when liquid level declines with distilled water.
4. wax-apple Water culture propagation method according to claim 1, is characterized in that: the wide 20-30cm of casing described in step (3), long 50-60cm, dark 20-25cm; Aperture 1.0cm on described cuttings fixed head.
5., according to wax-apple Water culture propagation method according to claim 1, it is characterized in that: the seedling medium described in step (6) by coconut palm chaff and peat soil by volume for 1:1 Homogeneous phase mixing forms.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107624400A (en) * | 2016-07-12 | 2018-01-26 | 郑德剑 | A kind of wax-apple grafting seedlings-growing method |
| CN108285387A (en) * | 2018-02-08 | 2018-07-17 | 金华市铁骑士生物科技有限公司 | Pear-tree grafting nutrient solution |
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| CN108285387A (en) * | 2018-02-08 | 2018-07-17 | 金华市铁骑士生物科技有限公司 | Pear-tree grafting nutrient solution |
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Application publication date: 20151202 |