CN103493659B - Method for quick breeding and seedling growing of pennisetum hydridum - Google Patents

Method for quick breeding and seedling growing of pennisetum hydridum Download PDF

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CN103493659B
CN103493659B CN201310432614.2A CN201310432614A CN103493659B CN 103493659 B CN103493659 B CN 103493659B CN 201310432614 A CN201310432614 A CN 201310432614A CN 103493659 B CN103493659 B CN 103493659B
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seedling
cuttage
hybrid giant
giant napier
seedling bed
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CN103493659A (en
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黎华寿
李锦开
黎健龙
易自成
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South China Agricultural University
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Abstract

The invention relates to a method for quick breeding and seedling growing of pennisetum hydridum. The method includes the following steps of establishing a seedling growing bed, selecting materials for being tested, carrying out seed stem cuttage, growing seedlings and leaving a garden. In the seed stem cuttage step, the lower cutting opening of a cuttage cutting wood stem segment is dipped into plant growth regulators and inserted into the seedling growing bed or a cuttage basin filled with matrixes, and the plant growth regulators are one kind of cytokinin, seaweed essence and rooting powder; the matrixes are sand or peat. According to the method for quick breeding and seedling growing of the pennisetum hydridum, seedling growth can be promoted without the limit of seasonality and can be bred all the year around, the qualified seedling percentage for quick breeding and seedling growing of the pennisetum hydridum is generally between 95% and 100%, moving and plantation survival rates are high, the method has a direct significance in solving the problems that the production is influenced due to the low survival rate of the directly planted pennisetum hydridum and seedlings are insufficient after overwintering, and important reference value is provided for preservation and breeding expanding for the pennisetum hydridum of grass-type good seedlings.

Description

A kind of hybrid giant napier rapid seedling cultivation method
Technical field
The invention belongs to non-tube rapid propagation technology field, be specifically related to a kind of hybrid giant napier rapid seedling cultivation method.
Background technology
Non-tube rapid propagation technology is outside test tube bottle, use micro-territory environment controlling technique, for plant propagation creates the environment such as temperature, light, water, gas, nutrition, hormone the most suitable, play the physiological potential that plant is maximum, the rudiment of plant gene of taking root is expressed as early as possible, thus the long seedling of the Rapid Rooting realizing plant, and by carrying out many generation circulations in the mode of Miao Fanmiao, form a kind of novel plant fast breeding technique in a large number with the business seedling of cultivating value.It is not tissue culture technique, but essence is the principle that conjunctive tissue cultivates fast breeding technique, is transformed by traditional cuttage technique.
Compared with traditional cuttage technique, non-tube rapid propagation technology have reproductive efficiency high, transplant and the advantage such as easily to survive, can also the breeding of some normal cutting propagations of amount reproduction can not successful plant.Utilize non-vitro techniques can not only overcome conventional seedbed system method vegetable material to be difficult to take root or seedling cost crosses high nursery obstacle, significantly improve rooting rate and the survival rate of nursery stock, a large amount of manpower can also be saved, reduce labor strength, meet anniversary nursery needs, improve Quality of Seedlings and production efficiency.Since successful exploitation, be widely deployed the every field in agricultural production, as the rapid propagation with low costs nursery of gourd, fruit and vegetable F1 generation hybrid seed, fruit tree tealeaves exempt from anvil soon numerous, greening seedling flowers anniversary numerous, economic forest soon container seedling forestation numerous etc. soon.
The main Propagation Methods of hybrid giant napier is vegetative propagation, and its growth characteristics are like sugarcane, but it is less than sugarcane to plant joint, and its easy desiccation, large more than sugarcane water requirement during plantation, on the earth's surface of drying, nature survival rate is extremely low.Simultaneously; current cultivated species cold resistance is poor; the continuous low temperature of about 0 DEG C can by hybrid giant napier plant and part root kill; cause low temperature in the wild cannot to be multiplied continuously without cannot normally pass the winter under special protection condition; to the non-line-of-sight error seedling growth test that hybrid giant napier seedling carries out; the seedling of hybrid giant napier can be allowed to produce energy fast seedling growing throughout the year, there is larger practice significance.By literature search, the non-Test tube seedlings technology of hybrid giant napier does not also have the report of research both at home and abroad.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide and a kind of promote kind of stem rudiment and growth of seedling, and by seasonal restriction, Zhou Nianjun can the hybrid giant napier rapid seedling cultivation method of numerous seedling.
For solving the problem, the technical solution adopted in the present invention is as follows:
A kind of hybrid giant napier rapid seedling cultivation method, is characterized in that comprising the following steps:
(1) seedling bed is set up: at built-in vertical 2 seedling beds of steelframe shed, flatten, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of about 20cm above, install atomizer above seedling bed bottom seedling bed;
(2) for examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, cane is cut into 1 every section joint, total length is about about 10cm, peels off whole blade simultaneously;
(3) stem cuttage is planted: choosing the health of the ripe plant hybrid giant napier at 6 monthly ages more than, saving for planting without the stem stalk of damage by disease and insect, now cane grows more than 10 joints, whole strain hybrid giant napier cane is cut down from earth's surface base portion, cane is mitered into the cuttage fringe bar of 1 every section joint by sections, under cuttage fringe bar stipes section, section dips in plant growth regulator, insert in matrix is housed seedling bed or cuttage basin in, cuttage gos deep into 2 ~ 3cm below seedling bed surface to planting stipes position, the degree of depth is 6 ~ 10cm, drench normal root water, sealing is covered, coverage shading net by White plastic film;
(4) nursery: control seedling bed temperature 25 ~ 32 DEG C, humidity 80 ~ 90%, nursery is penetrated in natural scattering illumination, cultivates hybrid giant napier cuttage seeding;
(5) garden is gone out: garden can be gone out when more than height of seedling 10cm.
As further technical scheme of the present invention, the size of described seedling bed is high 60cm, long 10m, inner width 1.2m.
As further technical scheme of the present invention, described plant growth regulator is the one in the basic element of cell division, algae essence and root-inducing powder.
Preferably, described plant growth regulator is the algae essence of 10mg/L.
Preferably, described plant growth regulator is the basic element of cell division of 15mg/L.
Preferably, described plant growth regulator is the root-inducing powder of 5mg/L.
As further technical scheme of the present invention, described matrix is husky or peat.
Compared to existing technology, beneficial effect of the present invention is: hybrid giant napier rapid seedling cultivation method of the present invention, growth of seedling can be promoted, and not by seasonal restriction, Zhou Nianjun can numerous seedling, and hybrid giant napier rapid seedling cultivation planting percent is generally 95% ~ 100%, mobile, planting survival rate is high, on solve survive the winter after hybrid giant napier directly plant the problem that the not enough impact of the low and seedling of survival rate produces there is direct meaning, to the preservation of this grass of hybrid giant napier good seed with expand and numerous there is important reference value.
Embodiment
Experimental field overview: be experimental field positioned at the peaceful western Experimental Base in Agricultural University Of South China Zengcheng (23 ° of 14 ' N, 113 ° of 38 ' E), belong to south subtropics monsoon climate, average temperature of the whole year 21.6 DEG C, the hottest is July, monthly mean temperature 29.4 DEG C, Extreme Maximum Temperature 38.6 DEG C; The coldest is January, monthly mean temperature 13.3 DEG C, Annual lowest climate temperature.Average annual rainfall is 1967.8mm, and rainwater mainly concentrates on for 4 ~ September, and every year from rainfall surge in April, 5 ~ June, rainfall was maximum, and October ~ March in next year is season short of rain, and average sunshine duration is 1707.2h.
Test material: be maternal with napier grass (Pennisetum K.Scbumacb), the heat that American pennisetum alopecuroides (Pennisetumtyphoideum Rich) is paternal hybrid grinds No. 4 hybrid giant napiers (Pennisetum hydridum), draws from Ning Xi proving ground, Guangdong Agricultural University Of South China Zengcheng for examination material.
Embodiment 1:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; For examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: section under above-mentioned stipes section is dipped in the algae essence of 10mg/L, insert in husky bed is housed seedbed (or cuttage basin alms bowl) in, cuttage kind stem top joint go deep into stromal surface 2 ~ 4cm, drench normal root water, be positioned over seedling bed, cover sealing by White plastic film, coverage shading net; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Embodiment 2:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; Stipes section is for examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, and cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: section under above-mentioned stipes section is dipped in the algae essence of 10mg/L, inserts in seedling bed (or the seedling-growing container alms bowl) Medium Culture that peat soil is housed, and cuttage kind stem top joint gos deep into stromal surface 2 ~ 4cm, drench normal root water, be positioned over seedling bed, cover sealing by White plastic film, coverage shading net; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Embodiment 3:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; Stipes section is for examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, and cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: section under above-mentioned stipes section is dipped in the basic element of cell division of 15mg/L, insert in husky bed is housed seedling bed (or cuttage basin) in, cuttage kind stem top joint go deep into stromal surface 2 ~ 4cm, drench normal root water, be positioned over seedling bed, cover sealing by White plastic film, coverage shading net; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Embodiment 4:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; Stipes section is for examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, and cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: section under above-mentioned stipes section is dipped in the basic element of cell division of 15mg/L, inserts in and be equipped with in the cuttage basin of peat soil, cuttage kind stem top joint gos deep into stromal surface 2 ~ 4cm, drench normal root water, be positioned over seedling bed, cover sealing by White plastic film, coverage shading net; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Embodiment 5:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; For examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: section under above-mentioned stipes section is dipped in the root-inducing powder of 5mg/L, insert in husky bed is housed seedling bed (or cuttage basin) in, cuttage kind stem top joint go deep into stromal surface 2 ~ 4cm, drench normal root water, be positioned over seedling bed, cover sealing by White plastic film, coverage shading net; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
Stipes section is in the survival rate of cuttage " Invest, Then Investigate " kind stem, and rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Embodiment 6:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; Stipes section is for examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, and cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: section under above-mentioned stipes section is dipped in the root-inducing powder of 5mg/L, insert in peat soil is housed seedling bed (or cuttage basin) in, cuttage kind stem top joint go deep into stromal surface 2 ~ 4cm, drench normal root water, be positioned over seedling bed, cover sealing by White plastic film, coverage shading net; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Comparative example 1:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cmcm above, above seedling bed, atomizer is installed; For examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: above-mentioned fringe bar stipes section is directly inserted in the seedling bed (or cuttage basin) that husky bed is housed, cuttage kind stem top joint gos deep into stromal surface 2 ~ 4cm, drenches normal root water, is positioned over seedling bed, covers sealing, coverage shading net by White plastic film; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Comparative example 2:
A kind of hybrid giant napier rapid seedling cultivation method, comprise the following steps: seedling bed is set up: at built-in vertical 2 the high 60cm of steelframe shed, long 10m, the seedling bed of the wide 1.2m of interior sky, flatten bottom seedling bed, spread the thick rubble of 10cm above as drainage blanket, and then lay the cultivation matrix (or seedling-growing container alms bowl) of 20cm above, above seedling bed, atomizer is installed; For examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, cane is cut into every section and comprises 1 joint, total length is about about 10cm, cuts off whole blade simultaneously; Tloig-cutlage: above-mentioned fringe bar stipes section is directly inserted in the seedling bed (or cuttage basin alms bowl) that husky bed is housed, cuttage kind stem top joint gos deep into stromal surface 2 ~ 4cm, drenches normal root water, is positioned over seedling bed, covers sealing, coverage shading net by White plastic film; Nursery: control 25 ~ 32 DEG C, seedbed temperature, 80 ~ 90% humidity ranges, cultivate hybrid giant napier cuttage seeding, nursery is penetrated in natural scattering illumination; Go out garden: garden can be gone out when more than height of seedling 30cm.
In the survival rate of cuttage " Invest, Then Investigate " kind stem, rooting rate, number of on average taking root, root is long, sprouting length, sprouting quantity.
Result and analysis:
1, the plant growth regulator of variable concentrations is on the impact of hybrid giant napier survival rate and rooting rate
As shown in Table 1, under earth culture or husky training matrix are cultivated, the hybrid giant napier seedling percent of plant growth regulator algae essence in 10mg/L, rooting rate all reach 100%, the 15mg/L basic element of cell division, 5mg/L root-inducing powder on the seedling percent of hybrid giant napier and rooting rate to affect result consistent.The impact effect that different substrates is cultivated is not obvious.Test shows, by the non-line-of-sight error technology of the growth regulator of low concentration, just can bring out a large amount of Cloned seedling in a short time, and does not affect its survival rate of transplanting and rooting rate.
The growth regulator of table 1 variable concentrations is on the impact of hybrid giant napier survival rate and rooting rate
2, different substrates cultivates lower plant growth regulator to the impact of the fast numerous growth of seedling of hybrid giant napier
Watch 2 sand training matrix cultivates lower plant growth regulator to the impact of the fast numerous seedling of hybrid giant napier
As seen from the results in Table 2, under sand training condition, the root of embodiment is grown, leaf is long, it is several to take root and germinative number is all good than comparative example, and wherein the result of embodiment 2 is slightly better than other embodiment process.
Watch 3 peat growing medium cultivates lower plant growth regulator to the impact of the fast numerous growth of seedling of hybrid giant napier
As seen from the results in Table 3, under peat growing medium condition of culture, the root of embodiment is long, leaf is long, take root number and germinative number better than comparative example, 5mg/L root-inducing powder is the strongest to the influence that hybrid giant napier seedling root is long, all reaches the significance of difference with control treatment.
Comprehensive above result of the test, under earth culture and Sha Pei matrix are cultivated, adds that plant growth regulator is long to the root of hybrid giant napier, leaf long, take root the facilitations such as number and germinative number comparatively obviously, all with contrast action effect and reach the significance of difference.The facilitation better effects if of 10mg/L algae essence, hybrid giant napier seedling percent, rooting rate all reach 100%.It can thus be appreciated that the growth of non-line-of-sight error seedling technology to hybrid giant napier seedling adding plant growth regulator has good application prospect.Result shows, under green house by the temperature humidity of intelligent automatic control, controlled by the optimizing regulation of environmental parameter, easily for hybrid giant napier creates a growing environment the suitableeest, promote growth of seedling, impel propagating materials to take root, improve rooting rate, and by seasonal restriction, Zhou Nianjun can numerous seedling, thus this technology have various plants expand numerous in the prospect applied.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, change and the replacement of any unsubstantiality that those skilled in the art does on basis of the present invention all belong to the present invention's scope required for protection.

Claims (3)

1. a hybrid giant napier rapid seedling cultivation method, is characterized in that comprising the following steps:
(1) seedling bed is set up: at built-in vertical 2 seedling beds of steelframe shed, flatten, spread the thick rubble of 10cm above as drainage blanket, install atomizer above seedling bed bottom seedling bed;
(2) for examination material selection: the hybrid giant napier cane that clip growing way is consistent with thickness, cane is cut into 1 every section joint, total length is 10cm, peels off whole blade simultaneously;
(3) plant stem cuttage: when hybrid giant napier cane grow 10 joints above time, whole strain hybrid giant napier cane is cut down from earth's surface base portion, cane is mitered into kind of a stem cuttage fringe bar by every section of 1 sections, under cuttage fringe bar stipes section, section dips in plant growth regulator, insert in matrix is housed seedling bed or cuttage basin in, cuttage gos deep into 2 ~ 3cm under seedling bed stromal surface to planting stipes position, and the degree of depth is totally 6 ~ 10cm; Drench normal root water, cover sealing by White plastic film, coverage shading net; Described plant growth regulator is the one in the root-inducing powder of the basic element of cell division of 15mg/L, the algae essence of 10mg/L, 5mg/L;
(4) nursery: control seedling bed temperature 25 ~ 32 DEG C, humidity 80 ~ 90%, nursery is penetrated in natural scattering illumination, cultivates hybrid giant napier cuttage seeding;
(5) garden is gone out: garden can be gone out when more than height of seedling 10cm.
2. hybrid giant napier rapid seedling cultivation method according to claim 1, is characterized in that: the size of described seedling bed is high 60cm, long 10m, inner width 1.2m.
3. hybrid giant napier rapid seedling cultivation method according to claim 1, is characterized in that: described matrix is husky or peat.
CN201310432614.2A 2013-09-22 2013-09-22 Method for quick breeding and seedling growing of pennisetum hydridum Active CN103493659B (en)

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