CN105104143A - Water planting cutting propagation method for myrtus communis - Google Patents
Water planting cutting propagation method for myrtus communis Download PDFInfo
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- CN105104143A CN105104143A CN201510549818.3A CN201510549818A CN105104143A CN 105104143 A CN105104143 A CN 105104143A CN 201510549818 A CN201510549818 A CN 201510549818A CN 105104143 A CN105104143 A CN 105104143A
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- cuttings
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- nutrient solution
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a water planting cutting propagation method for myrtus communis. The method includes the steps of collecting and treating a cutting slip, preparing a nutrient solution, preparing a cuttage container, conducting water planting cultivation, conducing management after cuttage and conducting acclimatization and transplant. The method includes the specific steps that a semi-lignified myrtus communis tender branch is selected, and then is inserted in the cuttage container filled with the nutrient solution for water planting cultivation after being subjected to trimming treatment, the culture condition is controlled, the acclimatization and transplant are conducted after the cutting slip growing into a young root is obtained, and accordingly a myrtus communis nursery stock is obtained. By the adoption of the method, female plants can be accumulated, so that mass micropropagation is condcuted. Meanwhile, the problem that cuttage seasons are limited by time is solved, cutting propagation can be carried out all the year around, the culture period is short, rooting is fast, environment controllability is good, the culture condition is stable, and the survival rate of cutting slips can reach 93%. The method is suitable for mass production and can be widely popularized, and good economic benefits, social benefits and ecological benefits are achieved.
Description
Technical field
The invention belongs to plant cuttage propagation field, relate to the cottage propagation of myrtle, be specifically related to a kind of myrtle Water culture propagation method.
Background technology
Myrtle is Myrtaceae Myrtle, and be the external evergreen shrubs introduced, blade edge is golden yellow, and flower is in white, and pistil is longer, and blade has fragrance, and gram refinement essential oil, fruit is in blue, and the seedlings of general 2-3 can be yielded positive results.This kind sight has peculiar fragrance well, again, and damage by disease and insect is also few, can be with application in Jiangsu and Zhejiang Provinces one.Myrtle should be widely used in township afforestation, is especially suitable for flower garden plantation.Can be used as
flower borderbackground is set, and plants in border, or plants before the enclosure wall faced south, and forms green barrier.Be used as
residential quarteror the high hedgerow of road, also have novel effect.Myrtle is owing to containing myrtenol, and have strong bactericidal effect, medical value is higher, right
tracheitisdeng
respiratory diseasecurative effect is better, also has calm, soporific function.
Prior art is because of maternal quantity not sufficient, so the seed amount of gathering is limited, and seed growing is sexual propagation, and the shape of the seedling that seed germination is grown up likely mutation occurs.Prior art also has and adopts the method for cuttage and seedling culture, but myrtle Cutting conditions and environment wayward, thus cause the instability of survival rate.
Summary of the invention
The present invention, in order to overcome the deficiencies in the prior art, provides a kind of cultivation period short, expands numerous fast, environment controllability is good, condition of culture is stablized, survival rate is high myrtle Water culture propagation method.
In order to realize foregoing invention object, the technical solution used in the present invention is as follows:
A kind of myrtle Water culture propagation method, comprise cuttings sampling and processing, the preparation of nutrient solution, the preparation of cuttage vessel, water planting cultivated, the operation of management and acclimatization and transplants after cuttage, the raw then semi-lignified that growth selection is healthy and strong, bud is good, disease-free or lignified myrtle young sprout, insert to be equipped with in the cuttage vessel of nutrient solution after pruning modes and carry out water planting cultivation, control condition of culture, carry out acclimatization and transplants after obtaining growing up to the cuttings of young root, obtain myrtle nursery stock; Its concrete operation step is as follows:
(1) cuttings sampling and processing: the spray selecting myrtle, the branch of the semi-lignified part of 8-10cm in the middle of intercepting, prunes growth 5-8cm as cuttings, leaves and takes cuttings top 1-3 sheet leaflet, extract other blade in cuttings more from lower to upper, cuttings bottom is cut into inclined-plane; Before cuttage, with the water rinse cuttings 0.5h of cleaning, take out after draining away the water, quickly dipped 10s in the mixed solution of basic for cuttings 2-3cm length is submerged NAA and indolebutyric acid;
(2) preparation of nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.4-1.5mg/L+GGR1.0-2.0mg/L+PG30-50mg/L+ sucrose 10g/L+ agar 2.0-5.0g/L+ active carbon 1.0-2.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.8-6.2;
(3) preparation of cuttage vessel: the casing selecting glass to make in cuttage indoor is as cuttage vessel, and pour nutrient solution in cuttage vessel, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and punch, as cuttings fixed head at cavities place card punch;
(4) water planting is cultivated: cuttings step (1) obtained is inserted in the aperture of cuttings fixed head, and makes cuttings inclination 30-45 °, is then placed in cuttage vessel;
(5) manage after cuttage: room temperature controls as 20-30 DEG C, indoor humidity controls as 80-100%, and solution temperature controls as 15-20 DEG C, and nutrient solution is changed in timing; With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat;
(6) acclimatization and transplants: when the cuttings young root of water planting grow to 1-2cm long time, implanting in seedling medium and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains myrtle nursery stock.
Preferably, its material content of mixed solution described in above step (1) is NAA250mg/L and indolebutyric acid 750mg/L.
Preferably, in above step (5), the frequency of oxygenation is every 7h ventilation 1h; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time when liquid level declines with distilled water.
Preferably, the wide 20-30cm of casing described in above step (3), long 50-60cm, dark 20-25cm; Aperture 1.0cm on described cuttings fixed head.
Preferably, the seedling medium described in above step (6) is formed by ash and perlite Homogeneous phase mixing.
Relative to prior art, the advantage that the present invention has and beneficial effect as follows:
1, the present invention prepares excellent nutrient solution, have adjusted the pH value benefiting myrtle and take root, and by the impact on myrtle cuttage root-taking such as nutritive element, plant growth regulator, improves cutting plantation; Meanwhile, in cottage propagation process, the condition such as temperature, humidity, ventilation is completely controlled, and nutrient component absorbing capacity is strong, and well developed root system, cutting plantation is high and stable.
2, the present invention utilizes homemade casing to carry out Water culture cultivation as cuttage vessel; female parent can be accumulated; thus it is numerous to carry out a large amount of expansion; overcome the problem that cuttage is subject to time restriction season simultaneously; all can carry out cottage propagation throughout the year, cuttings survival rate more than 93%, is applicable to large-scale production; can widely popularize, there are good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
A kind of myrtle Water culture propagation method, selects the spray of myrtle, the branch of the semi-lignified part of 8-10cm in the middle of intercepting, prune growth 5-6cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.4mg/L+GGR1.0mg/L+PG30mg/L+ sucrose 10g/L+ agar 2.0g/L+ active carbon 1.0g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.8.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 20cm of cuttage casing, long 50cm, dark 20cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3 ~ 5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 2-3cm length submerged is quickly dipped 10s in the mixed solution of NAA250mg/L and indolebutyric acid 750mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 30 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, room temperature controls as 20-25 DEG C, and indoor humidity controls as 80-90%, and solution temperature controls as 15-18 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implanting in the seedling medium by ash and perlite Homogeneous phase mixing and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains myrtle nursery stock.
Embodiment 2:
A kind of myrtle Water culture propagation method, selects the spray of myrtle, the branch of the semi-lignified part of 8-10cm in the middle of intercepting, prune growth 6-8cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.8mg/L+GGR1.5mg/L+PG40mg/L+ sucrose 10g/L+ agar 3.0g/L+ active carbon 2.0g/L, adds citric acid solution and nutrient solution pH value is adjusted to 6.0.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 25cm of cuttage casing, long 55cm, dark 20cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 2-3cm length submerged is quickly dipped 10s in the mixed solution of NAA250mg/L and indolebutyric acid 750mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 30 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, room temperature controls as 20-25 DEG C, and indoor humidity controls as 80-90%, and solution temperature controls as 15-18 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implanting in the seedling medium by ash and perlite Homogeneous phase mixing and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains myrtle nursery stock.
Embodiment 3:
A kind of myrtle Water culture propagation method, selects the spray of myrtle, the branch of the semi-lignified part of 8-10cm in the middle of intercepting, prune growth 5-8cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO1.2mg/L+GGR2.0mg/L+PG40mg/L+ sucrose 10g/L+ agar 4.0g/L+ active carbon 1.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.9.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 30cm of cuttage casing, long 60cm, dark 25cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 2-3cm length submerged is quickly dipped 10s in the mixed solution of NAA250mg/L and indolebutyric acid 750mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 45 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, it is 25 ~ 30 DEG C that room temperature controls, and it is 90 ~ 100% that indoor humidity controls, and solution temperature controls as 18-20 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implanting in the seedling medium by ash and perlite Homogeneous phase mixing and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains myrtle nursery stock.
Embodiment 4:
A kind of myrtle Water culture propagation method, selects the spray of myrtle, the branch of the semi-lignified part of 8-10cm in the middle of intercepting, prune growth 5-8cm as cuttings, leave and take cuttings top 1-3 sheet leaflet, then extract other blade in cuttings from lower to upper, cuttings bottom is cut into inclined-plane.The preparation of preparation nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO1.5mg/L+GGR2.0mg/L+PG50mg/L+ sucrose 10g/L+ agar 5.0g/L+ active carbon 2.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 6.2.
Before cuttage, at the indoor casing selecting glass to make of cuttage as cuttage vessel, the wide 30cm of cuttage casing, long 60cm, dark 25cm, the thick 0.5cm of glass; In cuttage vessel, pour nutrient solution into, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and be the hole of 1.0cm in cavities place card punch punching footpath, as cuttings fixed head.With the water rinse cuttings 0.5h of cleaning, take out after draining away the water, the content that cuttings base portion 2-3cm length submerged is quickly dipped 10s in the mixed solution of NAA250mg/L and indolebutyric acid 750mg/L.Cuttings after process is inserted in the hole of cuttings fixed head, and makes cuttings tilt 45 °, be then placed in cuttage vessel and carry out Water culture cultivation.In this incubation, room temperature controls as 25-30 DEG C, and indoor humidity controls as 90-100%, and solution temperature controls as 15-20 DEG C; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time change nutrient solution when liquid level declines with distilled water.With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat; The frequency of oxygenation is every 7h ventilation 1h.When the cuttings young root of water planting grow to 1-2cm long time, implant by ash and perlite Homogeneous phase mixing seedling medium in and be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains myrtle nursery stock.
Claims (5)
1. a myrtle Water culture propagation method, it is characterized in that: comprise cuttings sampling and processing, the preparation of nutrient solution, the preparation of cuttage vessel, water planting are cultivated, the operation of management and acclimatization and transplants after cuttage, select the myrtle spray of semi-lignified, insert to be equipped with in the cuttage vessel of nutrient solution after pruning modes and carry out water planting cultivation, control condition of culture, carry out acclimatization and transplants after obtaining growing up to the cuttings of young root, obtain myrtle nursery stock; Its concrete operation step is as follows:
(1) cuttings sampling and processing: the spray selecting myrtle, the branch of the semi-lignified part of 8-10cm in the middle of intercepting, prunes growth 5-8cm as cuttings, leaves and takes cuttings top 1-3 sheet leaflet, extract other blade in cuttings more from lower to upper, cuttings bottom is cut into inclined-plane; Before cuttage, with the water rinse cuttings 0.5h of cleaning, take out after draining away the water, quickly dipped 10s in the mixed solution of basic for cuttings 2-3cm length is submerged NAA and indolebutyric acid;
(2) preparation of nutrient solution: its material content of nutrient solution is: 1/2MS+IBAO0.4-1.5mg/L+GGR1.0-2.0mg/L+PG30-50mg/L+ sucrose 10g/L+ agar 2.0-5.0g/L+ active carbon 1.0-2.5g/L, adds citric acid solution and nutrient solution pH value is adjusted to 5.8-6.2;
(3) preparation of cuttage vessel: the casing selecting glass to make in cuttage indoor is as cuttage vessel, and pour nutrient solution in cuttage vessel, Depth Nutrient Solution is 3-5cm; And be cut into cuttage vessel size with flexible plastic cave dish, and punch, as cuttings fixed head at cavities place card punch;
(4) water planting is cultivated: cuttings step (1) obtained is inserted in the aperture of cuttings fixed head, and makes cuttings inclination 30-45 °, is then placed in cuttage vessel;
(5) manage after cuttage: it is 20 ~ 30 DEG C that room temperature controls, it is 80 ~ 100% that indoor humidity controls, and solution temperature controls as 15-20 DEG C, and nutrient solution is changed in timing; With oxygen pump and heating rod to the nutrient solution oxygenation in cuttage vessel, heat;
(6) acclimatization and transplants: when the cuttings young root of water planting grow to 1-2cm long time, implanting in seedling medium and to be colonizated in furrow, the degree of depth implanting matrix is 4cm; After transplanting, every day waters 1 water, successively decreases, avoid direct sunlight after 10 days, obtains myrtle nursery stock.
2. myrtle Water culture propagation method according to claim 1, is characterized in that: in step (1), its material content of described mixed solution is NAA250mg/L and indolebutyric acid 750mg/L.
3. myrtle Water culture propagation method according to claim 1, is characterized in that: in step (5), the frequency of oxygenation is every 7h ventilation 1h; Every 10d changes one time of nutrition liquid, and the degree of depth keeping nutrient solution is 3-5cm, supplements in time when liquid level declines with distilled water.
4. myrtle Water culture propagation method according to claim 1, is characterized in that: the wide 20-30cm of casing described in step (3), long 50-60cm, dark 20-25cm; Aperture 1.0cm on described cuttings fixed head.
5. according to myrtle Water culture propagation method according to claim 1, it is characterized in that: the seedling medium described in step (6) is formed by ash and perlite Homogeneous phase mixing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106332718A (en) * | 2016-08-25 | 2017-01-18 | 合肥市田然农业科技园有限公司 | Rapid loquat seedling culture method |
| CN106818443A (en) * | 2017-03-24 | 2017-06-13 | 卢鹏 | A kind of red maple bough water planting cuttage breeding method |
| CN106888912A (en) * | 2017-03-03 | 2017-06-27 | 福建省林业科学研究院 | A kind of horse-tail winter-spring season temperature control vegetative propagation technique method |
| CN108464233A (en) * | 2017-02-21 | 2018-08-31 | 重庆文理学院 | A kind of dual-purpose peach introduction and cultivation method of sight food |
| CN110199852A (en) * | 2019-07-08 | 2019-09-06 | 安徽省大地园林股份有限公司 | A kind of method of Japanese Premna water planting root induction |
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| CN108464233A (en) * | 2017-02-21 | 2018-08-31 | 重庆文理学院 | A kind of dual-purpose peach introduction and cultivation method of sight food |
| CN106888912A (en) * | 2017-03-03 | 2017-06-27 | 福建省林业科学研究院 | A kind of horse-tail winter-spring season temperature control vegetative propagation technique method |
| CN106888912B (en) * | 2017-03-03 | 2020-10-23 | 福建省林业科学研究院 | Temperature-controlled asexual propagation technical method for casuarina equisetifolia in winter and spring |
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| CN110199852A (en) * | 2019-07-08 | 2019-09-06 | 安徽省大地园林股份有限公司 | A kind of method of Japanese Premna water planting root induction |
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