CN103109724A - Water planting method for Chinese pine seeding suitable for experiment - Google Patents
Water planting method for Chinese pine seeding suitable for experiment Download PDFInfo
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Abstract
The invention discloses a water planting method for a Chinese pine seedling suitable for an experiment. The water planting method comprises the following steps: A. seed germination pre-cultivation, namely Chinese pine seeds are disinfected with 0.1% mercuric chloride, washed by running water for further use, clean wet sand is loaded in a cultivating device, and germination pre-cavitation of the Chinese pine seeds is conducted in general sand, B. adaptability cultivation, namely after the seeds are germinated, same-size and robust seedlings are chosen and transplanted into water for adaptability cultivation till seed cases drop off, C. growth of needles, namely after the seed cases drop off, the seedlings are transplanted to nutrient solution to cultivate, and two-wheeled needles grow after twenty days, and D. seedling collection, namely seedlings are collected according to experiment requests to be used in the experiment. The water planting method for the Chinese pine seeding suitable for the experiment has the advantages that soil is replaced by the nutrient solution to cultivate Chinese pine seedlings trough seed germination to Chinese pine growth, thus control and operation are easy, the growth of the Chinese pine seedlings is faster than soil culture, the growth of the Chinese pine seedlings is good, good seedlings are provided to the experiment, when the roots of the seedlings are under stress treatment, other factors of experimental treatment is controllable, the factor is single and strong in controllability, the Chinese pine seedlings are suitable for process of the experiment, and accuracy of experiment data is improved.
Description
Technical field
The present invention relates to the ciltivating process of a kind of Chinese pine seedling, the ciltivating process of especially a kind of Chinese pine seedling of suitable experiment.
Background technology
Chinese pine is a kind of woody plant, be grown to the master as experiment material with soil, because of the soil constituent complexity, portable germ, easily cause the Chinese pine damping off, rotten situation arranged during soil incubation, but the leaf performance is not obvious, the situation that therefore can only observe leaf in former experimentation judges that the quality of seedling may be incredible in a short time.Comparatively difficult to the operation of seedlings root Stress treatment in experimentation, and due to the soil own characteristic, make the accuracy of control treatment not strong, have a strong impact on accuracy and the reliability of experimental data; More difficult to the physiological index determining relevant to root in addition, easily hinder root take soil or perlite when the matrix culture experiment is got seedling, and need to consume a large amount of labours.
Summary of the invention
The ciltivating process that the purpose of this invention is to provide a kind of Chinese pine seedling of suitable experiment, the growth of the method from the seed germination to the Chinese pine more easily controlled and operates, and factor is single and controllability is strong, is fit to the carrying out of experiment, has improved the accuracy of experimental data.
In order to solve the problems of the technologies described above, the ciltivating process of the Chinese pine seedling of a kind of suitable experiment of the present invention comprises the following steps:
A, seed germination preculture: the Chinese pine seeds of selected full seed, big or small uniformity, with Chinese pine seeds with 0.1% mercury chloride sterilization 10min, rinse 15min standby under flowing water, fill clean damp sand with culture device, conventional sand is sprouted preculture, cover plastic film on culture device, cultivate between cultivating;
After B, seed germination, until seedling is high when growing to 3～4 cm, the seedling replanting of choosing the in the same size robust growth is made adaptability in the running water and is cultivated, and comes off to a kind shell;
C, plant after shell comes off, transfer in 1/2 Hoagland nutrient solution of ph value 6.0 and cultivate, nutrient solution replacing in every three days once, the 3h that ventilates every day cultivates under temperature 26-28 ℃, the condition of humidity 60%, can grow the two-wheeled needle after 20 days;
D, according to the experiment needs collect seedling for experiment in good time.
Described culture device adopts opaque plastic box or plastics, amplifies little suitable cystosepiment with holes in plastic box or plastics and is used for fixedly seedling, with the ventilation pump of little ventilation head.
Described Hoagland nutrient solution prescription is:
Four water-calcium nitrate 945 mg/L potassium nitrate 506 mg/L ammonium nitrate 80mg/L potassium dihydrogen phosphate 136mg/L magnesium sulfate 493mg/L iron salt solutions 2.5ml/L trace element 5ml/L pH=6.0
Described iron salt solutions: ferrous sulfate heptahydrate 2.78g/L disodium ethylene diamine tetraacetate 3.73g/L distilled water 500ml pH=5.5
Described trace element: potassium iodide 0.83mg/l boric acid 6.2mg/L manganese sulphate 22.3mg/L zinc sulphate 8.6mg/L sodium molybdate 0.25mg/L copper sulphate 0.025mg/L cobalt chloride 0.025mg/L
Owing to having adopted said method, advantage of the present invention is: cultivate the Chinese pine seedling with nutrient solution and replace soil cultivation, the growth from the seed germination to the Chinese pine is more easily controlled and operates, the Chinese pine seedling is faster than the soil incubation growth, well-grown is for experiment provides good seedling, when processing the Stress treatment of root, test the factor of processing other controlled, factor is single and controllability is strong, is fit to the carrying out of experiment, has improved the accuracy of experimental data.
Embodiment
The below is described in detail embodiments of the invention.Concrete grammar is as follows:
The ciltivating process of a kind of Chinese pine seedling of suitable experiment,
(1) seed germination preculture: the Chinese pine seeds of selected full seed, big or small uniformity, with 0.1% mercury chloride sterilization 10min, rinse 15min standby under flowing water, fill clean damp sand with basin, seed is placed on the sand surface, and the above spreads one deck fine sand, covers plastic film on basin, heat and moisture preserving is cultivated between cultivating.
(2) adaptability is cultivated: after the about week, seedling is high when growing to 3 ~ 4 cm, choose the seedling of in the same size robust growth, in the middle of absorbent cotton parcel young plant rhizome, be fixed on cystosepiment, be placed on and cultivate kind of a shell as adaptability in the ice chest that running water is housed and come off, approximately the 7d left and right, running water is changed once in the centre, can stuffiness.
(3) water planting: after the kind shell comes off, transfer in 1/2 Hoagland nutrient solution of ph value 6.0 and cultivate, (nutrient solution prescription: four water-calcium nitrate 945 mg/L potassium nitrate 506 mg/L ammonium nitrate 80mg/L potassium dihydrogen phosphate 136mg/L magnesium sulfate 493mg/L iron salt solutions 2.5ml/L, micro-5ml/L pH=6.0
Iron salt solutions: ferrous sulfate heptahydrate 2.78g/L disodium ethylene diamine tetraacetate 3.73g/L distilled water 500ml pH=5.5
Trace element: potassium iodide 0.83mg/l boric acid 6.2mg/L manganese sulphate 22.3mg/L zinc sulphate 8.6mg/L sodium molybdate 0.25mg/L copper sulphate 0.025mg/L cobalt chloride 0.025mg/L) the every 3d of nutrient solution changes once, will clean ice chest and Chinese pine seedling root when changing nutrient solution, 3h ventilates every day.Cultivate under temperature 26-28 ℃, the condition of humidity 60%.Approximately can grow the two-wheeled Astilbo idestabularis (Hemsl.) Engler after 20d.Namely can be used for test this moment.
Growing-seedling period with aquatic cultivation Chinese pine seedling is 45d, with the same period earth culture (soil: sand: the Chinese pine seedling of fertilizer=1:1:1) is compared, and water planting Chinese pine growth of seedling is healthy and strong, well developed root system.The ground material fresh weight of water planting Chinese pine is higher by 23.5% than earth culture, and under ground portion is high by 120%, and earth culture has bad root phenomenon (approximately 10%), and water planting does not have seedling death phenomenon.
Claims (5)
1. a ciltivating process that is fit to the Chinese pine seedling of experiment, is characterized in that, comprises the following steps:
A, seed germination preculture: the Chinese pine seeds of selected full seed, big or small uniformity, with Chinese pine seeds with 0.1% mercury chloride sterilization 10min, rinse 15min standby under flowing water, fill clean damp sand with culture device, conventional sand is sprouted preculture, cover plastic film on culture device, cultivate between cultivating;
After B, seed germination, until seedling is high when growing to 3～4 cm, the seedling replanting of choosing the in the same size robust growth is made adaptability in the running water and is cultivated, and comes off to a kind shell;
C, plant after shell comes off, transfer in 1/2 Hoagland nutrient solution of ph value 6.0 and cultivate, nutrient solution replacing in every three days once, the 3h that ventilates every day cultivates under temperature 26-28 ℃, the condition of humidity 60%, can grow the two-wheeled needle after 20 days;
D, according to the experiment needs collect seedling for experiment in good time.
2. the ciltivating process of the Chinese pine seedling of a kind of suitable experiment according to claim 1, it is characterized in that: described culture device adopts opaque plastic box or plastics, amplify little suitable cystosepiment with holes in plastic box or plastics and be used for fixedly seedling, with the ventilation pump of little ventilation head.
3. the ciltivating process of the Chinese pine seedling of a kind of suitable experiment according to claim 1, it is characterized in that: described Hoagland nutrient solution prescription is:
Four water-calcium nitrate 945 mg/L potassium nitrate 506 mg/L ammonium nitrate 80mg/L potassium dihydrogen phosphate 136mg/L magnesium sulfate 493mg/L iron salt solutions 2.5ml/L trace element 5ml/L pH=6.0.
4. the ciltivating process of the Chinese pine seedling of a kind of suitable experiment according to claim 3, is characterized in that: described iron salt solutions: ferrous sulfate heptahydrate 2.78g/L disodium ethylene diamine tetraacetate 3.73g/L distilled water 500ml pH=5.5.
5. the ciltivating process of the Chinese pine seedling of a kind of suitable experiment according to claim 3, is characterized in that: described trace element: potassium iodide 0.83mg/l boric acid 6.2mg/L manganese sulphate 22.3mg/L zinc sulphate 8.6mg/L sodium molybdate 0.25mg/L copper sulphate 0.025mg/L cobalt chloride 0.025mg/L.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325294A (en) * | 2015-10-23 | 2016-02-17 | 辽宁省林业科学研究院 | Pinus-contorta needle-leaved-bundle water culturing nutrient solution and method |
| CN105746322A (en) * | 2016-03-29 | 2016-07-13 | 河南省农业科学院经济作物研究所 | Method for quickly conserving and reproducing cotton by utilizing cotton leaves |
| CN105815196A (en) * | 2016-03-29 | 2016-08-03 | 河南省农业科学院经济作物研究所 | Method for rapid conservation propagation of black sesame by using black sesame leaves |
| CN106922513A (en) * | 2017-04-11 | 2017-07-07 | 安徽师范大学 | A kind of method for culturing seedlings |
| CN107827536A (en) * | 2017-10-18 | 2018-03-23 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting dragon spruce tree vigo(u)r quickly to recover |
| CN107853001A (en) * | 2017-11-09 | 2018-03-30 | 内蒙古农业大学 | A kind of nutrient solution for promoting the fast quick-recovery of Chinese pine tree vigo(u)r |
| CN107896690A (en) * | 2017-10-18 | 2018-04-13 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting the fast quick-recovery of pinus sylvestris var. mongolica tree vigo(u)r |
| CN113443934A (en) * | 2021-06-10 | 2021-09-28 | 江苏润芃园艺有限公司 | Soilless culture nutrient solution for black pine and application thereof |
| CN114230418A (en) * | 2022-01-07 | 2022-03-25 | 贵州大学 | Water culture nutrient solution and water culture method for pinus massoniana seedlings |
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| CN105325294A (en) * | 2015-10-23 | 2016-02-17 | 辽宁省林业科学研究院 | Pinus-contorta needle-leaved-bundle water culturing nutrient solution and method |
| CN105746322A (en) * | 2016-03-29 | 2016-07-13 | 河南省农业科学院经济作物研究所 | Method for quickly conserving and reproducing cotton by utilizing cotton leaves |
| CN105815196A (en) * | 2016-03-29 | 2016-08-03 | 河南省农业科学院经济作物研究所 | Method for rapid conservation propagation of black sesame by using black sesame leaves |
| CN105815196B (en) * | 2016-03-29 | 2019-06-28 | 河南省农业科学院经济作物研究所 | Utilize the method for the quick child care breeding sesame of sesame blade |
| CN105746322B (en) * | 2016-03-29 | 2019-06-28 | 河南省农业科学院经济作物研究所 | Utilize the method for the quick child care breeding cotton of cotton leaf |
| CN106922513A (en) * | 2017-04-11 | 2017-07-07 | 安徽师范大学 | A kind of method for culturing seedlings |
| CN107827536A (en) * | 2017-10-18 | 2018-03-23 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting dragon spruce tree vigo(u)r quickly to recover |
| CN107896690A (en) * | 2017-10-18 | 2018-04-13 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting the fast quick-recovery of pinus sylvestris var. mongolica tree vigo(u)r |
| CN107853001A (en) * | 2017-11-09 | 2018-03-30 | 内蒙古农业大学 | A kind of nutrient solution for promoting the fast quick-recovery of Chinese pine tree vigo(u)r |
| CN113443934A (en) * | 2021-06-10 | 2021-09-28 | 江苏润芃园艺有限公司 | Soilless culture nutrient solution for black pine and application thereof |
| CN114230418A (en) * | 2022-01-07 | 2022-03-25 | 贵州大学 | Water culture nutrient solution and water culture method for pinus massoniana seedlings |
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