CN103988764B - The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening - Google Patents
The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening Download PDFInfo
- Publication number
- CN103988764B CN103988764B CN201410200978.2A CN201410200978A CN103988764B CN 103988764 B CN103988764 B CN 103988764B CN 201410200978 A CN201410200978 A CN 201410200978A CN 103988764 B CN103988764 B CN 103988764B
- Authority
- CN
- China
- Prior art keywords
- grams per
- per liter
- day
- cuttage
- plantlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000338 in vitro Methods 0.000 title claims abstract description 17
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 13
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims abstract description 8
- 230000006698 induction Effects 0.000 title claims abstract description 7
- 239000003755 preservative agent Substances 0.000 claims description 15
- 230000002335 preservative effect Effects 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 14
- 230000003203 everyday effect Effects 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 3
- 229910002651 NO3 Inorganic materials 0.000 claims description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 3
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 239000004571 lime Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 235000015393 sodium molybdate Nutrition 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 235000009529 zinc sulphate Nutrition 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 208000035143 Bacterial infection Diseases 0.000 abstract description 3
- 208000022362 bacterial infectious disease Diseases 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000011159 matrix material Substances 0.000 description 3
- 239000003864 humus Substances 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses the method for the root induction of a kind of potato tissue culture seedling and hardening, the present invention adopts the seedlings root of cultivating out through the time in 5-6 week flourishing, stem stalk is sturdy, cauline leaf is in great numbers, adaptable after transplanting, for the production of next step virus-free basic potato seed is laid a good foundation.Simple to operate, efficiency is high, and the seedling-cultivating tray used and plastic crate can repeatedly use, and greatly reduce cost, effectively can control the propagation of bacterial disease, and the growing environment for plantlet in vitro also can regulate and control accurately.
Description
Technical field
The present invention relates to a kind of potato breeding method, particularly relate to the method for the root induction of a kind of potato tissue culture seedling and hardening.
Background technology
At present, the seedbed generally in Greenhouse is carried out for the hardening of potato tissue culture seedling, and root does not excise, and way is that the plantlet in vitro grown up to is taken out from blake bottle, some adopts the form of floating seedlings, plantlet in vitro cuttage is swum on cystosepiment on the nutrient solution of preparation; Some then cuttage cultivate in matrix, matrix is perlite, vermiculite, humus soil thrin or mixture, and spray nutritious liquor, and the seedling completing the hardening stage gets final product transplant planting.
The shortcoming of prior art is: 1, potato tissue culture seedling seedling after hardening is still healthy and strong not, and especially root system is undeveloped; 2, cost is high, operates more complicated; 3, bacterial disease once occur after large area propagate have a big risk; 4, use matrix as large in the difficulty of the thorough disinfection such as perlite, vermiculite, humus soil, reuse and having a big risk of disease occurs; 5, the growing environment condition of plantlet in vitro regulation and control as bad in temp. and humidity, nutrient solution concentration and acid-base value.
Summary of the invention
Object of the present invention is just the method providing the root induction of a kind of potato tissue culture seedling and hardening in order to solve the problem.
The present invention is achieved through the following technical solutions above-mentioned purpose:
The present invention includes following steps:
Step 1: according to the proportional arrangement nutrient solution of nitrate of lime 0.5 grams per liter, ammonium nitrate 0.044 grams per liter, dipotassium hydrogen phosphate 0.14 grams per liter, potassium sulfate 0.026 grams per liter, magnesium sulfate 0.13 grams per liter, ferrous sulfate 0.013 grams per liter, copper sulphate 0.002 grams per liter, manganese sulphate 0.0043 grams per liter, boric acid 0.0047 grams per liter, zinc sulphate 0.0014 grams per liter, sodium molybdate 0.0003 grams per liter, Babysafe 0.0063 grams per liter, the nutrient solution conductance configured is 1.0ms/cm, and acid-base value is 5.6;
Step 2: the root of plantlet in vitro is excised, extract the blade of middle and lower part, only retain 2-3 the blade on top, osculum along seedling-cultivating tray inserts in seedling-cultivating tray, ensure that the bottom of seedling is in nutrient solution, after the whole cuttage of the seedling-cultivating tray in plastic crate is full, with preservative film, plastic crate is sealed, during respectively after cuttage the 3rd, 6,9,12 day, preservative film is respectively cut open the openning of length and width all about 8 centimetres, 4 otch should be uniformly distributed at four of a preservative film angle; The every day of 8-12 days respectively after cuttage along otch to the plantlet in vitro of the inside water spray once; Within the 14th day after cuttage, remove preservative film;
Step 3: respectively removing the Adlerika 1 time spraying every day after preservative film 5% to plantlet in vitro, till being transplanted to seedbed;
Step 4: change nutrient solution during respectively after cuttage the 14th, 21,28,35 day, every day light application time 4 hours, 20 hours interlunations, until indoor 24 h light are until transplant to seedbed after after cuttage the 28th day, and the environmental temperature of indoor is set as 21 DEG C from start to finish.Be cultured to the 35 to 42 day and get final product transplant planting.
Beneficial effect of the present invention is:
The present invention is a kind of method that virus-free basic potato seed is produced, compared with prior art, the present invention adopts the seedlings root of cultivating out through the time in 5-6 week flourishing, stem stalk is sturdy, cauline leaf is in great numbers, adaptable after transplanting, for the production of next step virus-free basic potato seed is laid a good foundation.Simple to operate, efficiency is high, and the seedling-cultivating tray used and plastic crate can repeatedly use, and greatly reduce cost, effectively can control the propagation of bacterial disease, and the growing environment for plantlet in vitro also can regulate and control accurately.
Embodiment
The invention will be further described below:
The present invention includes following steps:
Step 1: according to the proportional arrangement nutrient solution of nitrate of lime 0.5 grams per liter, ammonium nitrate 0.044 grams per liter, dipotassium hydrogen phosphate 0.14 grams per liter, potassium sulfate 0.026 grams per liter, magnesium sulfate 0.13 grams per liter, ferrous sulfate 0.013 grams per liter, copper sulphate 0.002 grams per liter, manganese sulphate 0.0043 grams per liter, boric acid 0.0047 grams per liter, zinc sulphate 0.0014 grams per liter, sodium molybdate 0.0003 grams per liter, Babysafe 0.0063 grams per liter, the nutrient solution conductance configured is 1.0ms/cm, and acid-base value is 5.6;
Step 2: the root of plantlet in vitro is excised, extract the blade of middle and lower part, only retain 2-3 the blade on top, osculum along seedling-cultivating tray inserts in seedling-cultivating tray, ensure that the bottom of seedling is in nutrient solution, after the whole cuttage of the seedling-cultivating tray in plastic crate is full, with preservative film, plastic crate is sealed, during respectively after cuttage the 3rd, 6,9,12 day, preservative film is respectively cut open the openning of length and width all about 8 centimetres, 4 otch should be uniformly distributed at four of a preservative film angle; The every day of 8-12 days respectively after cuttage along otch to the plantlet in vitro of the inside water spray once; Within the 14th day after cuttage, remove preservative film;
Step 3: respectively removing the Adlerika 1 time spraying every day after preservative film 5% to plantlet in vitro, till being transplanted to seedbed;
Step 4: change nutrient solution during respectively after cuttage the 14th, 21,28,35 day, every day light application time 4 hours, 20 hours interlunations, until indoor 24 h light are until transplant to seedbed after after cuttage the 28th day, and the environmental temperature of indoor is set as 21 DEG C from start to finish.Be cultured to the 35 to 42 day and get final product transplant planting.
Through experiment, adopt facility of the present invention and method to carry out root induction and hardening to potato tissue culture seedling, transplant planting after 42 days, during transplant planting, growth of seedling is healthy and strong, and average plant height 24 centimetres, well developed root system, is white, and stem stalk is sturdy, and cauline leaf is in great numbers.
Claims (1)
1. a method for the root induction of potato detoxicating plantlet in vitro and hardening, is characterized in that, comprises the following steps:
Step 1: according to the proportional arrangement nutrient solution of nitrate of lime 0.5 grams per liter, ammonium nitrate 0.044 grams per liter, dipotassium hydrogen phosphate 0.14 grams per liter, potassium sulfate 0.026 grams per liter, magnesium sulfate 0.13 grams per liter, ferrous sulfate 0.013 grams per liter, copper sulphate 0.002 grams per liter, manganese sulphate 0.0043 grams per liter, boric acid 0.0047 grams per liter, zinc sulphate 0.0014 grams per liter, sodium molybdate 0.0003 grams per liter, Babysafe 0.0063 grams per liter, the nutrient solution conductance configured is 1.0ms/cm, and acid-base value is 5.6;
Step 2: the root of plantlet in vitro is excised, extract the blade of middle and lower part, only retain 2-3 the blade on top, osculum along seedling-cultivating tray inserts in seedling-cultivating tray, ensure that the bottom of seedling is in nutrient solution, after the whole cuttage of the seedling-cultivating tray in plastic crate is full, with preservative film, plastic crate is sealed, during respectively after cuttage the 3rd, 6,9,12 day, preservative film is respectively cut open the openning of length and width all about 8 centimetres, 4 otch should be uniformly distributed at four of a preservative film angle; The every day of 8-12 days respectively after cuttage along otch to the plantlet in vitro of the inside water spray once; Within the 14th day after cuttage, remove preservative film;
Step 3: respectively removing the Adlerika 1 time spraying every day after preservative film 5% to plantlet in vitro, till being transplanted to seedbed;
Step 4: change nutrient solution during respectively after cuttage the 14th, 21,28,35 day, every day light application time 4 hours, 20 hours interlunations, until indoor 24 h light are until transplant to seedbed after after cuttage the 28th day, and the environmental temperature of indoor is set as 21 DEG C from start to finish; Be cultured to the 35 to 42 day and get final product transplant planting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410200978.2A CN103988764B (en) | 2014-05-13 | 2014-05-13 | The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410200978.2A CN103988764B (en) | 2014-05-13 | 2014-05-13 | The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103988764A CN103988764A (en) | 2014-08-20 |
| CN103988764B true CN103988764B (en) | 2016-04-13 |
Family
ID=51303309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410200978.2A Expired - Fee Related CN103988764B (en) | 2014-05-13 | 2014-05-13 | The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103988764B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322360B (en) * | 2014-09-30 | 2018-04-27 | 云南省农业科学院经济作物研究所 | A kind of water culture of potato seedling growth-promoting method for root |
| CN105075602A (en) * | 2015-08-11 | 2015-11-25 | 成都易胜科生物科技有限公司 | Method for producing seed potatoes |
| CN105145367A (en) * | 2015-09-28 | 2015-12-16 | 天津市天兴佳业科技有限公司 | Subculturing method of virus-free potato test-tube plantlet |
| CN106171220A (en) * | 2016-07-04 | 2016-12-07 | 内蒙古格瑞得马铃薯种业有限公司 | A kind of potato fertilization method |
| CN110999790B (en) * | 2019-12-26 | 2021-08-31 | 云南师范大学 | Method for simultaneously inhibiting bacteria and prolonging subculture time of potato tissue culture seedlings and subculture method |
| CN112106660B (en) * | 2020-10-09 | 2021-11-23 | 山东宇泰生物种业有限公司 | Culture medium and culture method for improving potato propagation process efficiency |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550411A (en) * | 2011-12-31 | 2012-07-11 | 四川省农业科学院园艺研究所 | Method for producing pre-basic seeds of potatoes |
| CN102657081A (en) * | 2012-04-28 | 2012-09-12 | 四川省农业科学院作物研究所 | Method for culturing potato virus-free test-tube plantlets in water culture mode |
| CN103053393A (en) * | 2012-10-29 | 2013-04-24 | 黄少学 | Cultivation method for producing miniature potato seeds of virus-free potatoes |
| CN103159528A (en) * | 2013-03-13 | 2013-06-19 | 四川省农业科学院作物研究所 | Nutrient solution and method for producing potato breeder seeds by aeroponics |
-
2014
- 2014-05-13 CN CN201410200978.2A patent/CN103988764B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550411A (en) * | 2011-12-31 | 2012-07-11 | 四川省农业科学院园艺研究所 | Method for producing pre-basic seeds of potatoes |
| CN102657081A (en) * | 2012-04-28 | 2012-09-12 | 四川省农业科学院作物研究所 | Method for culturing potato virus-free test-tube plantlets in water culture mode |
| CN103053393A (en) * | 2012-10-29 | 2013-04-24 | 黄少学 | Cultivation method for producing miniature potato seeds of virus-free potatoes |
| CN103159528A (en) * | 2013-03-13 | 2013-06-19 | 四川省农业科学院作物研究所 | Nutrient solution and method for producing potato breeder seeds by aeroponics |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103988764A (en) | 2014-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103988764B (en) | The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening | |
| CN102550411B (en) | Method for producing pre-basic seeds of potatoes | |
| CN105284620B (en) | A kind of method that Superearly peach bybrid embryo saves seedling | |
| CN105693409A (en) | Flower soilless culture nutrient solution | |
| CN103988762B (en) | A kind of water planting transplanting method of Tissue-cultured apple seedling | |
| CN103053397B (en) | Micro-potato production method by mist culture method | |
| CN105075863B (en) | A kind of Paeonia papaveracea rapid propagation method | |
| CN1711825A (en) | Water floatation seedling method of cotton | |
| CN103430848A (en) | Growing method for propagating tulip | |
| CN105103714A (en) | Manual germination accelerating method and seedling raising method of Bletilla sfriata (Thunb.)Reiehb.f. | |
| CN106857256A (en) | The method that beautiful dew breeding potential is improved based on callus induction Regeneration Ways | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN106665044A (en) | Cultivation method of selenium-enriched tomato | |
| CN105123479A (en) | Water culture transplanting method for cherry tissue culture seedlings | |
| CN105028215A (en) | Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata | |
| CN104396759B (en) | The method that ash tree tissue cultures is bred fast | |
| CN104705194A (en) | Hormone-induced rapid propagation method of potamogeton pectinatus engineering seedlings | |
| CN106258958A (en) | A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling | |
| CN107493866A (en) | A kind of apocarya propagation method of cuttage | |
| CN103238523B (en) | Method for rapidly breeding tulip by using tulip scales | |
| CN104381133A (en) | Tissue culture breeding method of phlox subulata | |
| CN103907497A (en) | Rapid cutting propagation method of test-tube plum plantlets | |
| CN103081703A (en) | Tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method | |
| CN105379627A (en) | Sequoia sempervirens in vitro rooting culture method | |
| CN102487801B (en) | Method for soilless culture of bulblets of tissue culture seedlings of oriental lily |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Xinyong Inventor after: Bao Yuanyuan Inventor before: Bao Yuanyuan Inventor before: Zhang Xinyong Inventor before: Zheng Lu |
|
| COR | Change of bibliographic data | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160413 |