CN103081703A - Tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method - Google Patents

Tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method Download PDF

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Publication number
CN103081703A
CN103081703A CN2013100533548A CN201310053354A CN103081703A CN 103081703 A CN103081703 A CN 103081703A CN 2013100533548 A CN2013100533548 A CN 2013100533548A CN 201310053354 A CN201310053354 A CN 201310053354A CN 103081703 A CN103081703 A CN 103081703A
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cuttage
root
tetraploid
seedling
bottle
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CN2013100533548A
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常军
刘晓明
聂琳
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HENAN BENMU FEED ENGINEERING AND TECHNOLOGY RESEARCH CENTER
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HENAN BENMU FEED ENGINEERING AND TECHNOLOGY RESEARCH CENTER
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Abstract

The invention relates to a tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method. According to the method, sterile tetraploid robinia pseudoacacia tissue cultured multiplication seedling twigs are adopted as cutting wood, roots of the cutting wood are immersed in a rooting agent for no more than 30 seconds before cottage, and then the cutting wood is inserted in a potted tray filled with matrix for regular management, regeneration plants are obtained after 10-15 days, and the regeneration plants grow for 15-20 days into transplanting seedlings for transplanting. According to the tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method, cultivated plants are stable in heritability, flourishing in root system, strong, and high in survival rate of land transplanting.

Description

A kind of outer cuttage root-taking method of tetraploid locust group training seedling bottle
Technical field
The invention belongs to the plant cuttage root-taking technical field, be specifically related to a kind of outer cuttage root-taking method of tetraploid locust group training seedling bottle.
Background technology
Extensive plantation along with tetraploid locust, its fast-growing that has, drought resisting, barren-resistant, Salt And Alkali Tolerance, fertility are strong etc., and good characteristic is not fully exerted in China's forestry ecological benefit, having obtained good ecological benefits, economic benefit and social benefit, is the first-selected fine tree species such as forests for windbreak and sand-fixation, field safeguarding forest, green barren hill and conceding the land to forestry.The development and utilization of tetraploid locust is played positive facilitation to the development of forest industry.Yet because tetraploid locust belongs to the difficulty seeds that take root, in production, the roots that adopt numerous more, grafting, and the mode of cuttage is carried out seedling-wood breeding.But traditional modes of reproduction is subject to seasonal restrictions, and can not breed in the winter time, affects to production, thereby has restricted applying of tetraploid locust.
Summary of the invention
The object of the invention is to provide a kind of tetraploid locust group training seedling bottle outer cuttage root-taking method, and the plant heritability that the method is cultivated is stable, well developed root system, robust plant, and the field-transplanting survival rate is higher.
For achieving the above object, the present invention adopts following technical scheme:
A kind of outer cuttage root-taking method of tetraploid locust group training seedling bottle, the method selects aseptic tetraploid locust group training propagation seedling spray as plugged ear, before cuttage, the base portion of plugged ear first is immersed in and is no more than 30 seconds in root-growing agent, then cuttage is carried out Routine Management in the cave dish of matrix is housed, and obtains regeneration plant (regeneration plant obtains transplanted seedling through growth in 15-20 days and can transplant) after 10-15 days.
Concrete, described root-growing agent is preferably indolebutyric acid (IBA) solution of concentration 1000-1500 mg/L.
Described matrix is the mixture that is comprised of the turfy soil of weight 1:1 and perlite.Before cuttage, the matrix in the dish of cave can prevent the generation of cuttage seeding damage by disease and insect in advance with the aqueous solution disinfection that contains 0.2% potassium permanganate and 0.01% carbendazim.Matrix adopts turfy soil and perlite to mix and uses, and humidity is agglomerating to hold, fall into ground distributor after unclamping scatters and be advisable.
described Routine Management is specially: the plugged ear cuttage is watered once permeable after the cave dish of matrix is housed, cuttage degree of depth 1-1.5cm, then change over to full exposure and automatically control in the booth of spraying management, MS liquid nutrient medium (in order to sufficient nutritive element to be provided) after spraying the carbendazol (morbidity of avoiding infection) of weight percent concentration 0.01-0.03% one week and diluting 100 times each 2-3 time, the Shi Yizhou that begins to take root sprays potassium dihydrogen phosphate aqueous solution 2-3 time (forming in order to promote the root lignification) of weight percent concentration 0.1-0.5%, obtain regeneration plant after 10-15 days.
When carrying out in the following manner when spraying MS liquid nutrient medium (without sucrose), effect is better: after the macroelement mother liquor in medium and micro-mother liquor are respectively diluted 100 times, sooner or later alternately spray, week spray gets final product for 2-3 time.
Concrete, the selection growth is sturdy, the plant color is dark green, well-fed tetraploid locust group training propagation seedling spray, downcut as plugged ear from callus base portion 0.5cm with aseptic cutter, and assurance spray cutting length 3-3.5cm, the plugged ear otch is level and smooth.
Adopt the inventive method can make tetraploid locust group training propagation seedling spray can obtain regeneration plant in 10-15 days, rooting rate reaches more than 70%, has solved the problem of traditional cuttage root-taking difficulty, really reaches fast numerous purpose.This method cuttage can be transplanted after one month, well developed root system (radical 3-5 root), and about the long 4cm of root, robust plant, the field-transplanting survival rate is more than 90%.
Compare with existing method, cuttage root-taking method of the present invention has following advantage:
1) be not subject to seasonal restrictions.The characteristics of the inventive method maximum are that propagating materials derives from group training seedling, are not subject to seasonal restrictions.Tradition cuttage meeting can't be carried out cutting propagation because of factors such as plant physiology situation and weather conditions, the season limit factor is extremely strong, and change in the booth of full exposure auto spraying control and management after the cuttage of tetraploid locust group training propagation aseptic seedling, the water regime of monitoring plant is at any time also carried out moisture and is regulated, humidity is controlled more than 75%, temperature remains between the 22-25 degree and (prevents that excess Temperature from causing the cuttage seeding perish), for optimal condition is created in its growth, thereby improve the rooting rate of propagating materials, throughout the year all can numerous seedling.
2) save production cost.Traditional tetraploid locust group training seedling need to culture of rootage one month, hardening just can be transplanted after one week in root media.Adopt the inventive method cuttage spray not need to cultivate through root media, can obtain regeneration plant in 10-15 days after cuttage, saved human and material resources and financial resources, thereby reduced production cost, improved economic benefit.
The plant of 3) adopting the inventive method to cultivate, well developed root system, transplanting survival rate is high.When traditional method tissue culture is transplanted, be easy to root is caused damage, thereby affect transplanting survival rate.The inventive method adopts loose soilless substrate, is more conducive to the absorption of plant nutrition moisture, can not cause damage to root during transplanting, has improved transplanting survival rate.
4) convenient operation management, easy to learn.The inventive method adopts full exposure auto spraying management in booth, waters, sprays medicine, fertilising etc. and all can realize automatic management, and is easy and simple to handle.This seedling growing process has not only been saved labour's input, has also reduced production cost, has improved productivity effect.The regeneration plant survival rate that adopts the inventive method to cultivate is high, heritability stable, can reach at short notice fast numerous effect, obtains whole plant, has saved production cost, has improved productivity effect.Increased new way for tetraploid locust expands numerous mode, exploitation prospect is very extensive.
Description of drawings
Fig. 1 is for adopting (the approximately 15 days) picture of taking root of the inventive method gained cuttage seeding;
Fig. 2 is for adopting (approximately 30 days) picture of the inventive method gained transplanted seedling;
Fig. 3 is the photo that survives of transplanted seedling.
Embodiment
Below by specific embodiment, technical solution of the present invention is described, but protection scope of the present invention is not limited to this.
Embodiment 1
A kind of outer cuttage root-taking method of tetraploid locust group training seedling bottle, the method comprises the steps:
1) plugged ear is selected: the selection growth is sturdy, the plant color is dark green, well-fed tetraploid locust group training propagation seedling spray, downcut as plugged ear from callus base portion 0.5cm with aseptic cutter, and assurance spray cutting length 3-3.5cm, the plugged ear otch is level and smooth;
2) cuttage: will first be immersed in as the base portion of the tetraploid locust group of plugged ear training propagation aseptic seedling spray in the indolebutyric acid solution of concentration 1000 mg/L 15 seconds, then cuttage is carried out Routine Management in the cave dish of matrix (described matrix is mixed by turfy soil and the perlite of weight 1:1, and matrix is disinfection in advance) is housed;
3) Routine Management: the plugged ear cuttage is watered once permeable after the cave dish of matrix is housed, cuttage degree of depth 1.5cm, then change over to automatically to control in the booth of managing of spraying with full exposure and (keep 25 ℃ of temperature in booth, the control environment of humidity 75-80%), one all spraying concentration 0.03%(percentage by weights) carbendazol and the MS liquid nutrient medium of dilution after 100 times each 3 times, all spraying concentration 0.4%(percentage by weights when beginning to take root) potassium dihydrogen phosphate aqueous solution 2 times, obtain regeneration plant after 12 days, the regeneration plant growth approximately obtains transplanted seedling after 20 days and can transplant.Should in time clear up the rotten branch of deadwood during Routine Management, guarantee the slotting machine cleaning.
Embodiment 2
A kind of outer cuttage root-taking method of tetraploid locust group training seedling bottle, the method comprises the steps:
1) plugged ear is selected: the selection growth is sturdy, the plant color is dark green, well-fed tetraploid locust group training propagation seedling spray, downcut as plugged ear from callus base portion 0.5cm with aseptic cutter, and assurance spray cutting length 3-3.5cm, the plugged ear otch is level and smooth;
2) cuttage: will first be immersed in as the base portion of the tetraploid locust group of plugged ear training propagation aseptic seedling spray in the indolebutyric acid solution of concentration 1500 mg/L 25 seconds, then cuttage is carried out Routine Management in the cave dish of matrix (described matrix is mixed by turfy soil and the perlite of weight 1:1, and matrix is disinfection in advance) is housed;
3) Routine Management: the plugged ear cuttage is watered once permeable after the cave dish of matrix is housed, cuttage degree of depth 1.2cm, then change over to automatically to control in the booth of managing of spraying with full exposure and (keep 25 ℃ of temperature in booth, the control environment of humidity 75-80%), one all spraying concentration 0.01%(percentage by weights) carbendazol and the MS liquid nutrient medium of dilution after 100 times each 2 times, all spraying concentration 0.2%(percentage by weights when beginning to take root) potassium dihydrogen phosphate aqueous solution 3 times, obtain regeneration plant after 15 days, the regeneration plant growth approximately obtains transplanted seedling after 15 days and can transplant.

Claims (5)

1. a tetraploid locust group is trained the outer cuttage root-taking method of seedling bottle, it is characterized in that, the method selects aseptic tetraploid locust group training propagation seedling spray as plugged ear, before cuttage, the base portion of plugged ear first is immersed in and is no more than 30 seconds in root-growing agent, then cuttage is carried out Routine Management in the cave dish of matrix is housed, and obtains regeneration plant after 10-15 days.
2. the outer cuttage root-taking method of tetraploid locust group training seedling bottle as claimed in claim 1, is characterized in that, described root-growing agent is the indolebutyric acid solution of concentration 1000-1500 mg/L.
3. the outer cuttage root-taking method of tetraploid locust group training seedling bottle as claimed in claim 1, is characterized in that, described matrix is the mixture that is comprised of the turfy soil of weight 1:1 and perlite.
4. tetraploid locust group as claimed in claim 1 is trained the outer cuttage root-taking method of seedling bottle, it is characterized in that, described Routine Management is specially: the plugged ear cuttage is watered once permeable after the cave dish of matrix is housed, cuttage degree of depth 1-1.5cm, then change over to automatically controlling in the booth of spraying management, MS liquid nutrient medium after spraying the carbendazol of weight percent concentration 0.01-0.03% one week and diluting 100 times each 2-3 time, the Shi Yizhou that begins to take root sprays potassium dihydrogen phosphate aqueous solution 2-3 time of weight percent concentration 0.1-0.5%, obtain regeneration plant after 10-15 days.
5. tetraploid locust group as claimed in claim 1 is trained the outer cuttage root-taking method of seedling bottle, it is characterized in that, the selection growth is sturdy, the plant color is dark green, well-fed tetraploid locust group training propagation seedling spray, downcut as plugged ear from callus base portion 0.5cm with aseptic cutter, guarantee spray cutting length 3-3.5cm, the plugged ear otch is level and smooth.
CN2013100533548A 2013-02-19 2013-02-19 Tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method Pending CN103081703A (en)

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CN104705063A (en) * 2015-03-31 2015-06-17 山东省林业科学研究院 Cutting rooting method for hard branches of robinia pseudoacacia
CN105009908A (en) * 2015-07-29 2015-11-04 广西壮族自治区林业科学研究院 Out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings
CN105766547A (en) * 2016-05-04 2016-07-20 梁友盛 Plug seedling foresting method for broad-leaf tree species
CN108684346A (en) * 2018-05-03 2018-10-23 贵阳中医学院 A kind of cuttage breeding method of Sabia parviflora Wall.ex Roxb
CN108848977A (en) * 2017-12-20 2018-11-23 江苏省林业科学研究院 A kind of promotion blueberry tissue-cultured seedling rooting method

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104705063A (en) * 2015-03-31 2015-06-17 山东省林业科学研究院 Cutting rooting method for hard branches of robinia pseudoacacia
CN105009908A (en) * 2015-07-29 2015-11-04 广西壮族自治区林业科学研究院 Out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings
CN105009908B (en) * 2015-07-29 2017-11-24 广西壮族自治区林业科学研究院 The method of the outer forming seedling through one step culture of China fir unrooted test tube seedling bottle
CN105766547A (en) * 2016-05-04 2016-07-20 梁友盛 Plug seedling foresting method for broad-leaf tree species
CN108848977A (en) * 2017-12-20 2018-11-23 江苏省林业科学研究院 A kind of promotion blueberry tissue-cultured seedling rooting method
CN108684346A (en) * 2018-05-03 2018-10-23 贵阳中医学院 A kind of cuttage breeding method of Sabia parviflora Wall.ex Roxb

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