CN108848977A - A kind of promotion blueberry tissue-cultured seedling rooting method - Google Patents
A kind of promotion blueberry tissue-cultured seedling rooting method Download PDFInfo
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- CN108848977A CN108848977A CN201810738799.2A CN201810738799A CN108848977A CN 108848977 A CN108848977 A CN 108848977A CN 201810738799 A CN201810738799 A CN 201810738799A CN 108848977 A CN108848977 A CN 108848977A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The present invention relates to a kind of methods for promoting blueberry tissue culture seedling rooting, mainly solve the technical problem that existing blueberry tissue culture seedling rooting has that culture program is more, survival rate is low, the period is long etc..Include the following steps：（1）Bottle seedling selection；（2）Hardening；（3）Tissue culture transplantation of seedlings；（4）It is managed after transplanting.The present invention establishes effective blueberry tissue culture outside sprout-cultivating-bottle radication technology, optimizes its tissue culture rapid propagation system.The technology needs not move through program of taking root in tissue culture bottle, takes root and tames while carrying out, 25 ~ 30d seedling base portion grows a large amount of root systems.Healthy and strong rooted seedling is obtained after 2 months.Compared with taking root in conventional bottles, growing-seedling period shortens 1 month or more, transplanting survival rate is greater than 95%.And the outside sprout-cultivating-bottle technology can reduce production cost 40% or so, saves 50% or more tissue culture space, is of great significance for blueberry tissue culture intensive manufacture.
Description
Technical field
The present invention relates to the method for culturing seedlings of blueberry, concretely relate to the side of the quick outside sprout-cultivating-bottle of blueberry tissue-cultured seedling
Method.
Background technique
Blueberry (Vaccinium bracteatum) it is Ericaceae (Ericacece) genus vaccinium（Vaccinium）Often
Green shrub or dungarunga, are widely distributed in low altitude area mountainous region on the south the Changjiang river, and habitat is mostly under mountainous region border, sparse woods or in bushes.Crow
Meal tree has long applicating history and wide development prospect in China, and leaf can impregnate glutinous rice and cook, especially in one band of Jiangsu and Zhejiang Provinces
Just there is the custom of food " black meal " from ancient times；It is sour-sweet after berry is mature, jam and wine brewing can be manufactured；Plant is rich in flavonoids object
The ingredients such as matter, amino acid, vitamin and mineral element have important medical value；Tree performance is graceful, and color is full of variety, and has
There is exploitation at the potentiality of potted landscape and Colored foliage plants.Early in the 1940s, the states such as America and Europe, Japan begin to pay attention to blueberry
Edible, medical value, has developed health foodstuff series and drug.
Two aspect of medicinal efficacy and medicinal ingredient is focused primarily upon to the research report of blueberry both at home and abroad at present, and to it
The correlative studys such as Lycopersicum esculentum and breeding are less.Wherein in relation to blueberry cutting propagation in terms of studies have shown that current blueberry
Technique on Cuttage Propagation is still immature, and survival rate is lower.Tissue cultures are a kind of quickly and effectively vegetative propagation approach, can effectively be expanded
Increase excellent variety, a large amount of high-quality blueberry nursery stocks are provided in a short time, have pushed the rapid development of blueberry industry.However it is existing
Research and practice have shown that, the problems such as big with cost, the period is long, transplanting survival rate is low of taking root in blueberry tissue-cultured seedling bottle.
Summary of the invention
Goal of the invention：The present invention provides a kind of methods for promoting blueberry tissue culture seedling rooting, are blueberry excellent variety
Scale quick propagation provides technical support.
Technical solution：To achieve the above object, present invention employs technical solution below, a kind of promotion blueberry tissue cultures
The method of seedling rooting, includes the following steps：
（1）Bottle seedling selection：The blueberry tissue-cultured seedling to grow fine is selected, highly in 8-10 cm or so, branch is healthy and strong, is refined
Seedling domestication；
（2）Hardening：Blueberry tissue-cultured seedling is first placed into seeding room, seeding room is to scatter based on light, intensity of illumination 3000-4000
Lux, temperature are controlled at 15-25 °C, after culture 5-7 days, open bottle cap, continue culture 2-3 days in seeding room；
（3）The outer cuttage of tissue-cultured seedling bottle：Seedling is taken out out of bottle, the culture medium for being sticked to plant base portion is cleaned, cuts, pluck by single plant
Except bottom jaundice blade breakage；Every plant is cut into 3-4 cm stem with bud, water of taking root is dipped before cuttage, cuttage depth is with 1-1.5 public affairs
Point, the matrix of wherein cuttage selects peat；Sprinkling moisture during cuttage frequently, to ensure that seedling does not wither；
（4）It is managed after cuttage：It is sprinkled profoundly water after cuttage, controls 80% or so, prop up a hut with preservative film above seedling,
1-2 weeks recession canopy is cultivated, a water was poured every three days, sprays a foliar fertilizer every two weeks, monthly sprays a fungicide.25~ 30 d
Seedling base portion grows a large amount of root systems, healthy and strong blueberry seedling is obtained after 2 months, and planted in transplanting to nutritive cube.
Preferably, step（3）In take root water using ABT root-inducing powder 1, concentration is 500 mg/L, and dipping the time is 5
Second, impregnate 1 ~ 2 cm of tissue-cultured seedling base portion, the preparation method of every liter of rooting agent solution：By a small amount of ethyl alcohol of 500 mg ABT root-inducing powders
Dissolution, is settled to 1 L with clear water；
Preferably, step（3）Middle cuttage vessel selects plastic seeding culturing plate, and matrix accounts for 3/4 volume of seedlings nursing plate.
Preferably, step（3）In peat 800 times of carbendazim processing disinfection；800 times of carbendazim is：Take concentration
50% 1 g of carbendazim dilutes gained with the water of 800 mL.
Preferably, step（4）Cultivation temperature control is at 20 ~ 25 DEG C, and humidity keeps 85% or more, and light intensity is 1500 ~ 2000
Lux, light application time 16h/d.
Preferably, step（4）Middle foliar fertilizer be flower piece and banbo you it is two different be used alternatingly, fungicide is
The carbendazim and Bravo confection of 0.1 %.
Beneficial effect：The present invention has the advantage that in terms of existing technologies：
The present invention provides a kind of technology and methods that can refer to for the quick outside sprout-cultivating-bottle of blueberry tissue-cultured seedling, has following excellent
Point：
1, the present invention is compared with conventional method for tissue culture, eliminates link of taking root in bottle, growing-seedling period shorten 1 month with
On, it is easy to operate, it is practical；
2, it takes root in the present invention and tames while carrying out, rooting rate is greater than 95%, and well developed root system, growth of seedling is healthy and strong, transplant survival
Rate 100%, seedling consistency are good；
3, the outside sprout-cultivating-bottle technology in the present invention can reduce production cost 40% or so, save 50% or more tissue culture space, for
The promotion of blueberry tissue culture industrialization level is of great significance.
Specific embodiment
Below in conjunction with specific example, the specific embodiment that the present invention will be described in detail：
Example 1：A method of promoting blueberry tissue culture seedling rooting, this method carries out as follows：
（1）Bottle seedling selection：The blueberry tissue-cultured seedling to grow fine is selected, highly in 8-10 cm or so, branch is healthy and strong, is refined
Seedling domestication.
（2）Hardening：Blueberry tissue-cultured seedling is first placed into seeding room, to scatter based on light, intensity of illumination is for seeding room
3000Lux, temperature are controlled at 25 °C, after culture 7 days, open bottle cap, continue culture 3 days in seeding room.
（3）The outer cuttage of tissue-cultured seedling bottle：Seedling is taken out out of bottle, the culture medium for being sticked to plant base portion is cleaned, is cut by single plant
Under, extract bottom jaundice blade breakage；Every plant is cut into 3-4 cm stem with bud, water of taking root is dipped before cuttage, cuttage depth is with 1-
1.5 centimeters, the matrix of wherein cuttage selects perlite；Sprinkling moisture during cuttage frequently, to ensure that seedling does not wither；
800 times of carbendazim processing disinfections of peat；800 times of carbendazim is：Take 1 g of carbendazim of concentration 50% with 800 mL's
Water dilution gained；
The preparation method of every liter of aqueous solution of taking root：500 mg ABT root-inducing powder 1 is dissolved with a small amount of ethyl alcohol, with clear water constant volume
To 1 L, water of taking root uses ABT root-inducing powder 1, and concentration is 500 mg/L, and dipping the time is 5 seconds, impregnates tissue-cultured seedling base portion 1 ~ 2
Cm, cuttage vessel select plastic seeding culturing plate, and matrix accounts for 3/4 volume of seedlings nursing plate；It needs to use peat when cuttage, peat is needed with 800
The processing disinfection of times carbendazim；800 times of carbendazim is：Take the water dilution gained of 1 g of carbendazim of concentration 50%, 800 mL.
（4）It is managed after cuttage：It is sprinkled profoundly water after cuttage, controls 80% or so, preservative film Zhi Yi little is used above seedling
Canopy cultivates 1-2 weeks recession canopy, a water was poured every three days, sprays a foliar fertilizer every two weeks, monthly sprays a fungicide.25 d
Seedling base portion grows a large amount of root systems.Healthy and strong blueberry seedling is obtained after 2 months, and is planted in transplanting to nutritive cube；Culture
Temperature control is at 20 DEG C, and humidity keeps 85% or more, light intensity 2000lx, light application time 16h/d.Foliar fertilizer be flower piece and
Banbo that is two different to be used alternatingly, and fungicide is the carbendazim and Bravo confection of 0.1 %.
Example 2：A method of promoting blueberry tissue culture seedling rooting, this method carries out as follows：
（1）Prepare root-growing agent：
The preparation method of every liter of rooting agent solution is as follows：500 mg ABT root-inducing powder 1 is dissolved with a small amount of ethyl alcohol, uses clear water
It is settled to 1 L；
（2）Transplanting medium prepares：Perlite is fitted into during plastic seeding culturing plate educates, is sprinkled profoundly water spare；Every cube of matrix 20g 50%
Carbendazol wettable powder carries out disinfection sterilization；
（3）Bottle seedling selection：The blueberry tissue-cultured seedling to grow fine is selected, highly in 8-10 cm or so, branch is healthy and strong, is refined
Seedling domestication；
（4）Hardening：Blueberry tissue-cultured seedling is first placed into seeding room, to scatter based on light, intensity of illumination is 4000 Lux for seeding room,
Temperature is controlled at 15 °C, after culture 5 days, opens bottle cap, continues culture 2 days in seeding room；
（5）The outer cuttage of tissue-cultured seedling bottle：Seedling is taken out out of bottle, the culture medium for being sticked to plant base portion is cleaned, cuts, pluck by single plant
Except bottom jaundice blade breakage；Every plant is cut into 3-4 cm stem with bud, water of taking root is dipped before cuttage, cuttage depth is with 1-1.5 public affairs
Point, the matrix of wherein cuttage selects peat；Sprinkling moisture during cuttage frequently, to ensure that seedling does not wither；
800 times of carbendazim processing disinfections of peat；800 times of carbendazim is：Take 1 g of carbendazim of concentration 50% with 800 mL's
Water dilution gained；
The preparation method of every liter of aqueous solution of taking root：500 mg ABT root-inducing powder 1 is dissolved with a small amount of ethyl alcohol, with clear water constant volume
To 1 L, water of taking root uses ABT root-inducing powder 1, and concentration is 500 mg/L, and dipping the time is 5 seconds, impregnates tissue-cultured seedling base portion 1 ~ 2
Cm, cuttage vessel select plastic seeding culturing plate, and matrix accounts for 3/4 volume of seedlings nursing plate；It needs to use peat when cuttage, peat is needed with 800
The processing disinfection of times carbendazim；800 times of carbendazim is：Take the water dilution gained of 1 g of carbendazim of concentration 50%, 800 mL.
（6）It is managed after cuttage：It is sprinkled profoundly water after cuttage, controls 80% or so, preservative film Zhi Yi little is used above seedling
Canopy cultivates 1-2 weeks recession canopy, a water was poured every three days, sprays a foliar fertilizer every two weeks, monthly sprays a fungicide.30 d
Seedling base portion grows a large amount of root systems.Healthy and strong blueberry seedling is obtained after 2 months, and is planted in transplanting to nutritive cube.Culture
Temperature control is at 25 DEG C, and humidity keeps 85% or more, light intensity 1500lx, light application time 16h/d.Foliar fertilizer be flower piece and
Banbo that is two different to be used alternatingly, and fungicide is the carbendazim and Bravo confection of 0.1 %.
Pass through thousands of secondary blueberry tissue-cultured seedling Rootings, compared with taking root in bottle, the technology in above-mentioned two embodiment
It can reduce 40% or more production cost, save 50% or more tissue culture space, averagely 1 month tissue culture period of shortening or more, it is raw
Root rate is greater than 95%, and well developed root system, growth of seedling is healthy and strong, and transplanting survival rate 100%, seedling consistency is good, fast to blueberry tissue culture
The optimization of traditional font system has important technology meaning.
The present invention is disclosed with preferred embodiments above, so it is not intended to limiting the invention, all to use equivalent replacement or wait
The technical solution that mapping mode obtains is imitated, is within the scope of the present invention.
Claims (8)
1. a kind of promotion blueberry tissue-cultured seedling rooting method, which is characterized in that include the following steps：
（1）Bottle seedling selection：The blueberry tissue-cultured seedling to grow fine is selected, highly in 8-10 cm or so, branch is healthy and strong, is refined
Seedling domestication；
（2）Hardening：Blueberry tissue-cultured seedling is first placed into seeding room, seeding room is to scatter based on light, intensity of illumination 3000-
4000 Lux, temperature are controlled at 15-25 °C, after culture 5-7 days, open bottle cap, continue culture 2-3 days in seeding room；
（3）The outer cuttage of tissue-cultured seedling bottle：Tissue-cultured seedling is taken out out of bottle, the culture medium for being sticked to plant base portion is cleaned, is cut by single plant,
Extract bottom jaundice blade breakage；Every plant is cut into 3-4 cm stem with bud, water of taking root is dipped before cuttage, cuttage depth is with 1-1.5
The matrix of centimetre, wherein cuttage selects perlite；Sprinkling moisture during cuttage frequently, to ensure that tissue-cultured seedling is not withered；
（4）It is managed after cuttage：It is sprinkled profoundly water after cuttage, film is covered above seedling, cultivate 1-2 weeks recession film, it is then fixed
Foliar fertilizer, fungicide are applied in phase watering, and 25 ~ 30 d seedling base portions grow a large amount of root systems, and healthy and strong blueberry is obtained after 2 months
Seedling, and planted in transplanting to nutritive cube.
2. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：The step（3）In
Water of taking root uses ABT root-inducing powder 1, and concentration is 500 mg/L, and dipping the time is 5 seconds, impregnates 1 ~ 2 cm of tissue-cultured seedling base portion.
3. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：Every liter of aqueous solution of taking root
Preparation method：500 mg ABT root-inducing powder 1 is dissolved with a small amount of ethyl alcohol, is settled to 1 L with clear water.
4. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：Step（3）When middle cuttage
Container selects plastic seeding culturing plate, and matrix accounts for 3/4 volume of seedlings nursing plate.
5. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：The step（3）In
It needs to use peat when cuttage, peat is needed with 800 times of carbendazim processing disinfections；800 times of carbendazim is：Take the more of concentration 50%
Bacterium 1 g of spirit dilutes gained with the water of 800 mL.
6. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：The step（4）Training
Temperature control is supported at 20 ~ 25 DEG C, humidity keeps 85% or more, and light intensity is 1500 ~ 2000lx, light application time 16h/d.
7. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：The step（4）In
Foliar fertilizer be flower piece and banbo you it is two different be used alternatingly, fungicide is the carbendazim and Bravo mixing medicine of 0.1 %
Agent.
8. promotion blueberry tissue-cultured seedling rooting method according to claim 1, it is characterised in that：The step 4）In periodically
It waters, apply foliar fertilizer, fungicide, i.e., poured a water every three days, spray a foliar fertilizer every two weeks, monthly spray a fungicide.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601158A (en) * | 2019-01-29 | 2019-04-12 | 江苏省林业科学研究院 | A kind of cuttage breeding method of silver magpie tree |
| CN110612903A (en) * | 2019-10-22 | 2019-12-27 | 安徽师范大学 | Method for improving tissue culture subculture rooting of vaccinium bracteatum |
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