CN106258958A - A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling - Google Patents
A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling Download PDFInfo
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- CN106258958A CN106258958A CN201610641774.1A CN201610641774A CN106258958A CN 106258958 A CN106258958 A CN 106258958A CN 201610641774 A CN201610641774 A CN 201610641774A CN 106258958 A CN106258958 A CN 106258958A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling, including the clip of material of taking root, the preparation of facility of taking root, the configuration of nutritional solution, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plants period management step.The present invention is taken root in simulating Fructus Pruni pseudocerasi test tube Seedling bottle environment, makes culturing young plants be allowed to take root in substrate that relatively close, with nutritional solution, after progressively remove nutritional solution, plastic foil, be directly entered the substrate culture stage.The process that the method is taken root in incorporating tissue cultured seedling bottle and transplanted, makes to take root in culturing young plants directly, transplanting medium outer at bottle, simplifies production link, reduce production cost, improve emergence rate.This technical method is simple, and production cost is low, and planting percent is high, is presented as direct economic benefit on producing.
Description
Technical field
The present invention relates to field of plant tissue culture technique, a kind of method being specifically related to Fructus Pruni pseudocerasi tissue cultured seedling outside sprout-cultivating-bottle.
Background technology
In recent years, along with the fast development of science and technology, plant tissue culture technique has been enter into the production application stage, extensively
It is applied to flowers, the large-scale rapid propagation in vitro of fruit tree.Plant tissue culture detoxification nursery stock is the most numerous can be at manual control condition's next year four
Season produces, and obtains a large amount of group training detoxification nursery stock at short notice.But tissue cultured seedling aseptically, in special culture media repeatedly
Switching is cultivated, and production cost is high；By the transplanting of its Seedling of taking root, from indoor to outdoor, by the low light level in bottle, constant temperature, near saturated wet
Degree, gnotobasis gradually convert to natural environmental condition, and production environment is required height, and Temperature and Humidity Control difficulty is big, is restriction group
The bottleneck that training detoxic seedling scale, industrialization produce.
Summary of the invention
The technical problem to be solved is high for production cost of taking root in bottle in prior art, transplants environment control
A kind of Fructus Pruni pseudocerasi tissue cultured seedling outside sprout-cultivating-bottle technology that difficulty processed is big and restricts the problem of Fructus Pruni pseudocerasi tissue cultured seedling factorial praluction and provides, this
Method can make Fructus Pruni pseudocerasi tissue cultured seedling rooting rate reach more than 90%, and production facility device simple, and simple to operate, production cost is low, goes out
Seedling rate is high.
Adopt the following technical scheme that for solving the technical problem of the present invention
A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: neatly putting the high nutritive cube of high 10cm-12cm in flat pallet container, alms bowl is built-in
Enter the Vermiculitum of 4cm-6cm, make Vermiculitum surfacing, pallet injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Pruni pseudocerasi subculture tissue cultured seedling of more than 30 days is transported in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 1.5cm-2.5cm, plucks base
Portion 2-4 sheet lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the individual plant seedling sheared is inserted ready flat pallet container by 2cm × 2 cm line space
In interior nutritive cube, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in nutrition
In the space relatively closed in alms bowl, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then at flat pallet
Container injects the high nutritional solution of taking root of 1cm-2cm, carries out root culture；
D, period management of taking root: take root the stage after culturing young plants cuttage, temperature indoor temperature is maintained between 20-30 DEG C, and humidity keeps
More than 80%；
E, seedling exercising period management: root culture, after 20 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off after being cultivated for 4-6 days the plastic sheeting on nutritive cube or nutritive cube is moved to ground cultivate, in greenhouse
70% sunshade net shelters from heat or light, and humidity is maintained at more than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is in the nutritive cube of soil and decomposed manure, waters permeable in time, makes nursery stock gradually adapt to open country growing environment, wait seedling long
During to 5cm-10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Nutritional solution of taking root in described step a and step c is for consisting of: containing ammonium nitrate in every liter of nutritional solution of taking root
NH4NO31650mg, potassium nitrate KNO31900mg, potassium dihydrogen phosphate KH2PO4170 mg, sulphuric acid U.S. MgSO4·7H2O370mg, chlorine
Change calcium CaCl22H2O 440mg, potassium iodide KI0.41mg, boric acid H3BO33.1mg, manganese sulfate MnSO4·4H2O 11.1 mg, sulfur
Acid zinc ZnSO4·7H2O4.3mg, sodium molybdate Na2MoO4·2H2O0.125mg, copper sulfate CuSO4·5H2O0.0125 mg, chlorination
Cobalt CoCl2·6H2O0.0125mg, indolebutyric acid IBA0.5mg, heteroauxing IAA0.4mg.
Temperature sprayable decreasing temperature and increasing humidity time higher in described step d, or shelter from heat or light with 70% sunshade net, when wherein spraying, can be by
Mist is sprayed directly on on the plastic foil of nutritive cube, to keep moisture content in pallet.
In described step f, wherein the mass ratio of soil and decomposed manure is 1:0.3.
Described flat pallet container is along high 2cm-4cm.
This technology is taken root in having deducted tissue cultured seedling bottle and the link of bottle transplantation of seedlings of taking root, and considerably reduces tissue cultured seedling and produces
Cost, the method that simultaneously improve again a kind of Fructus Pruni pseudocerasi tissue cultured seedling outside sprout-cultivating-bottle of emergence rate.
The present invention is taken root in simulating Fructus Pruni pseudocerasi test tube Seedling bottle environment, makes culturing young plants that relatively close, with the base of nutritional solution
Matter is allowed to take root, after progressively remove nutritional solution, plastic foil, be directly entered the substrate culture stage.The method incorporates tissue cultured seedling
The process taken root in Ping and transplant, makes to take root in culturing young plants directly, transplanting medium outer at bottle, simplifies production link, reduce
Production cost, improves emergence rate.This technical method is simple, and production cost is low, and planting percent is high, is presented as directly on producing
Economic benefit.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated:
Take root nutritional solution for consisting of: containing ammonium nitrate NH in every liter of nutritional solution of taking root4NO31650mg, potassium nitrate
KNO31900mg, potassium dihydrogen phosphate KH2PO4170 mg, sulphuric acid U.S. MgSO4·7H2O370mg, calcium chloride CaCl22H2O 440mg,
Potassium iodide KI0.41mg, boric acid H3BO33.1mg, manganese sulfate MnSO4·4H2O 11.1 mg, zinc sulfate ZnSO4·7H2O4.3mg、
Sodium molybdate Na2MoO4·2H2O0.125mg, copper sulfate CuSO4·5H2O0.0125 mg, cobaltous chloride CoCl2·
6H2O0.0125mg, indolebutyric acid IBA0.5mg, heteroauxing IAA0.4mg.
Embodiment 1
A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: select the flat pallet container of the convenient carrying of size, flat pallet container along high 2cm,
Neatly put the high nutritive cube of high 12cm in flat pallet container, in nutritive cube, load the high Vermiculitum of 6cm, make Vermiculitum surface put down
Whole, the flat pallet container loading Vermiculitum injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Pruni pseudocerasi subculture tissue cultured seedling of more than 30 days is drawn in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 1.5cm, plucks base portion 2～4
Sheet lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the group training individual plant seedling sheared is inserted ready flat pallet by 2cm × 2 cm line space
In nutritive cube in container, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in
In the space relatively closed in nutritive cube, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then flat
Pallet container injects the high nutritional solution of 2cm, carries out root culture；
D, period management of taking root: cuttage little seedling rooting stage, temperature indoor temperature be maintained between 20-30 DEG C, humidity be maintained at 80% with
On, sprayable decreasing temperature and increasing humidity when temperature is higher, during spraying, mist can be sprayed directly on on the plastic foil of nutritive cube, to keep flat
Moisture content in pallet container；
E, seedling exercising period management: root culture, after 20 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off the plastic sheeting on nutritive cube after being cultivated for 6 days, in greenhouse, 70% sunshade net shelters from heat or light, and humidity is maintained at
More than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is for soil: decomposed manure mass ratio is in the nutritive cube of 1:0.3, waters permeable in time, makes nursery stock gradually adapt to open country growth
Environment, when waiting seedling to grow to 5cm～10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Embodiment 2
A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: select the flat pallet container of the convenient carrying of size, flat pallet container along high 4cm,
Neatly put the high nutritive cube of high 10cm in flat pallet container, in nutritive cube, load the high Vermiculitum of 4cm, make Vermiculitum surface put down
Whole, the flat pallet container loading Vermiculitum injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Pruni pseudocerasi subculture tissue cultured seedling of more than 30 days is drawn in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 2.5cm, plucks base portion 2～4
Sheet lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the group training individual plant seedling sheared is inserted ready flat pallet by 2cm × 2 cm line space
In nutritive cube in container, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in
In the space relatively closed in nutritive cube, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then flat
Pallet container injects the high nutritional solution of 1cm, carries out root culture；
D, period management of taking root: cuttage little seedling rooting stage, temperature indoor temperature be maintained between 20-30 DEG C, humidity be maintained at 80% with
On, sprayable decreasing temperature and increasing humidity when temperature is higher, during spraying, mist can be sprayed directly on on the plastic foil of nutritive cube, to keep flat
Moisture content in pallet container；
E, seedling exercising period management: root culture, after 20 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off the plastic sheeting on nutritive cube after being cultivated for 4 days, in greenhouse, 70% sunshade net shelters from heat or light, and humidity is maintained at
More than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is for soil: decomposed manure mass ratio is in the nutritive cube of 1:0.3, waters permeable in time, makes nursery stock gradually adapt to open country growth
Environment, when waiting seedling to grow to 5cm～10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Embodiment 3
A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: select the flat pallet container of the convenient carrying of size, flat pallet container along high 4cm,
Neatly put the high nutritive cube of high 10cm in flat pallet container, in nutritive cube, load the high Vermiculitum of 4cm, make Vermiculitum surface put down
Whole, the flat pallet container loading Vermiculitum injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Pruni pseudocerasi subculture tissue cultured seedling of more than 30 days is drawn in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 2.0cm, plucks base portion 2-4 sheet
Lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the group training individual plant seedling sheared is inserted ready flat pallet by 2cm × 2 cm line space
In nutritive cube in container, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in
In the space relatively closed in nutritive cube, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then flat
Pallet container injects the high nutritional solution of 1cm, carries out root culture；
D, period management of taking root: cuttage little seedling rooting stage, temperature indoor temperature be maintained between 20-30 DEG C, humidity be maintained at 80% with
On, shelter from heat or light with 70% sunshade net when temperature is higher；
E, seedling exercising period management: root culture, after 20 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, after being cultivated for 5 days, nutritive cube is moved to ground cultivate, in the greenhouse in continue 70% sunshade net shelter from heat or light, humidity holding
Manage more than 80%；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is for soil: decomposed manure mass ratio is in the nutritive cube of 1:0.3, waters permeable in time, makes nursery stock gradually adapt to open country growth
Environment, when waiting seedling to grow to 5cm-10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Claims (5)
1. the outside sprout-cultivating-bottle method of a Fructus Pruni pseudocerasi tissue cultured seedling, it is characterised in that: include the take root clip of material, the standard of facility of taking root
Standby, the configuration of nutritional solution, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: neatly putting the high nutritive cube of high 10cm-12cm in flat pallet container, alms bowl is built-in
Enter the Vermiculitum of 4cm-6cm, make Vermiculitum surfacing, pallet injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Pruni pseudocerasi subculture tissue cultured seedling of more than 30 days is transported in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 1.5cm-2.5cm, plucks base
Portion 2-4 sheet lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the individual plant seedling sheared is inserted ready flat pallet container by 2cm × 2 cm line space
In interior nutritive cube, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in nutrition
In the space relatively closed in alms bowl, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then at flat pallet
Container injects the high nutritional solution of taking root of 1cm-2cm, carries out root culture；
D, period management of taking root: take root the stage after culturing young plants cuttage, temperature indoor temperature is maintained between 20-30 DEG C, and humidity keeps
More than 80%；
E, seedling exercising period management: root culture, after 20 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off after being cultivated for 4-6 days the plastic sheeting on nutritive cube or nutritive cube is moved to ground cultivate, in greenhouse
70% sunshade net shelters from heat or light, and humidity is maintained at more than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is in the nutritive cube of soil and decomposed manure, waters permeable in time, makes nursery stock gradually adapt to open country growing environment, wait seedling long
During to 5cm-10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
The outside sprout-cultivating-bottle method of a kind of Fructus Pruni pseudocerasi tissue cultured seedling the most according to claim 1, it is characterised in that: described step a and
Nutritional solution of taking root in step c is for consisting of: containing ammonium nitrate NH in every liter of nutritional solution of taking root4NO31650mg, potassium nitrate
KNO31900mg, potassium dihydrogen phosphate KH2PO4170 mg, sulphuric acid U.S. MgSO4·7H2O370mg, calcium chloride CaCl22H2O 440mg,
Potassium iodide KI0.41mg, boric acid H3BO33.1mg, manganese sulfate MnSO4·4H2O 11.1 mg, zinc sulfate ZnSO4·7H2O4.3mg、
Sodium molybdate Na2MoO4·2H2O0.125mg, copper sulfate CuSO4·5H2O0.0125 mg, cobaltous chloride CoCl2·
6H2O0.0125mg, indolebutyric acid IBA0.5mg, heteroauxing IAA0.4mg.
The outside sprout-cultivating-bottle method of a kind of Fructus Pruni pseudocerasi tissue cultured seedling the most according to claim 1 and 2, it is characterised in that: described step d
Middle temperature is sprayable decreasing temperature and increasing humidity time higher, or shelters from heat or light with 70% sunshade net, when wherein spraying, mist can be sprayed directly on to nutritive cube
Plastic foil on, to keep moisture content in pallet.
The outside sprout-cultivating-bottle method of a kind of Fructus Pruni pseudocerasi tissue cultured seedling the most according to claim 1 and 2, it is characterised in that: described step f
In wherein soil and the mass ratio of decomposed manure are 1:0.3.
The outside sprout-cultivating-bottle method of a kind of Fructus Pruni pseudocerasi tissue cultured seedling the most according to claim 1 and 2, it is characterised in that: described flat
Pallet container is along high 2cm-4cm.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494543A (en) * | 2017-08-01 | 2017-12-22 | 山东大丰园农业有限公司 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication |
| CN108848977A (en) * | 2017-12-20 | 2018-11-23 | 江苏省林业科学研究院 | A kind of promotion blueberry tissue-cultured seedling rooting method |
| CN109220806A (en) * | 2018-11-06 | 2019-01-18 | 云南省红河热带农业科学研究所 | A kind of papaya seedling rooting and the method for transplanting |
| CN112189566A (en) * | 2020-11-12 | 2021-01-08 | 天水市果树研究所 | Rapid breeding method of cherry seedlings for rootstocks |
| CN113455399A (en) * | 2021-08-13 | 2021-10-01 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Method for rooting rosaceous cherry plants outside bottle |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668959A (en) * | 2012-06-14 | 2012-09-19 | 浙江师范大学 | Rapid ex vitro rooting method for blueberry tissue culture seedlings and rooting culture matrix |
| CN103371103A (en) * | 2013-07-30 | 2013-10-30 | 杭州植物园 | Rapid propagation method for tissue culture of Rhododendron delavayi Franch |
| CN103461121A (en) * | 2013-09-04 | 2013-12-25 | 广西壮族自治区林业科学研究院 | Ex-vitro rooting method for tissue culture seedlings of pinus massoniana |
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
-
2016
- 2016-08-08 CN CN201610641774.1A patent/CN106258958A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668959A (en) * | 2012-06-14 | 2012-09-19 | 浙江师范大学 | Rapid ex vitro rooting method for blueberry tissue culture seedlings and rooting culture matrix |
| CN103371103A (en) * | 2013-07-30 | 2013-10-30 | 杭州植物园 | Rapid propagation method for tissue culture of Rhododendron delavayi Franch |
| CN103461121A (en) * | 2013-09-04 | 2013-12-25 | 广西壮族自治区林业科学研究院 | Ex-vitro rooting method for tissue culture seedlings of pinus massoniana |
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
Non-Patent Citations (1)
| Title |
|---|
| 范志强等: "促进马扎德甜樱桃砧木组培苗生根的研究", 《落叶果树》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494543A (en) * | 2017-08-01 | 2017-12-22 | 山东大丰园农业有限公司 | A kind of root-growing agent and its outside sprout-cultivating-bottle method for cherry tissue culture outside sprout-cultivating-bottle radication |
| CN108848977A (en) * | 2017-12-20 | 2018-11-23 | 江苏省林业科学研究院 | A kind of promotion blueberry tissue-cultured seedling rooting method |
| CN109220806A (en) * | 2018-11-06 | 2019-01-18 | 云南省红河热带农业科学研究所 | A kind of papaya seedling rooting and the method for transplanting |
| CN112189566A (en) * | 2020-11-12 | 2021-01-08 | 天水市果树研究所 | Rapid breeding method of cherry seedlings for rootstocks |
| CN112189566B (en) * | 2020-11-12 | 2022-10-04 | 天水市果树研究所 | Rapid breeding method of cherry seedlings for stocks |
| CN113455399A (en) * | 2021-08-13 | 2021-10-01 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Method for rooting rosaceous cherry plants outside bottle |
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Application publication date: 20170104 |