CN106212237A - A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling - Google Patents
A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling Download PDFInfo
- Publication number
- CN106212237A CN106212237A CN201610641619.XA CN201610641619A CN106212237A CN 106212237 A CN106212237 A CN 106212237A CN 201610641619 A CN201610641619 A CN 201610641619A CN 106212237 A CN106212237 A CN 106212237A
- Authority
- CN
- China
- Prior art keywords
- seedling
- root
- taking root
- tissue
- nutritional solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling, including the clip of material of taking root, the preparation of facility of taking root, the configuration of nutritional solution, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plants period management step.The present invention is taken root environment in simulating examination Fructus Mali pumilae test tube Seedling bottle, makes culturing young plants be allowed to take root in substrate that relatively close, with nutritional solution, after progressively remove nutritional solution, plastic foil, be directly entered the substrate culture stage.The process that the method is taken root in incorporating tissue cultured seedling bottle and transplanted, makes to take root in culturing young plants directly, transplanting medium outer at bottle, simplifies production link, reduce production cost, improve emergence rate.This technical method is simple, and production cost is low, and planting percent is high, is presented as direct economic benefit on producing.
Description
Technical field
The present invention relates to field of plant tissue culture technique, a kind of side relating to Tissue-cultured apple seedling outside sprout-cultivating-bottle
Method, this method can make Tissue-cultured apple seedling rooting rate reach more than 90%, and production facility device simple, simple to operate, production cost
Low, emergence rate is high.
Background technology
In recent years, along with the fast development of science and technology, plant tissue culture technique has been enter into the production application stage, extensively
It is applied to flowers, the large-scale rapid propagation in vitro of fruit tree.Plant tissue culture detoxification nursery stock is the most numerous can be at manual control condition's next year four
Season produces, and obtains a large amount of group training detoxification nursery stock at short notice.But tissue cultured seedling aseptically, in special culture media repeatedly
Switching is cultivated, and production cost is high；By the transplanting of its Seedling of taking root, from indoor to outdoor, by the low light level in bottle, constant temperature, near saturated wet
Degree, gnotobasis gradually convert to natural environmental condition, and production environment is required height, and Temperature and Humidity Control difficulty is big, is restriction group
The bottleneck that training detoxic seedling scale, industrialization produce.
Summary of the invention
The technical problem to be solved is high for production cost of taking root in bottle in prior art, transplants environment control
Difficulty processed is big and causes tissue cultured seedling factorial praluction problem, it is provided that a kind of tissue cultured seedling outside sprout-cultivating-bottle technology.This technology deducts
Take root in tissue cultured seedling bottle and the link of bottle transplantation of seedlings of taking root, considerably reduce the cost that tissue cultured seedling produces, improve again simultaneously
A kind of method of the Tissue-cultured apple seedling outside sprout-cultivating-bottle of emergence rate.
Adopt the following technical scheme that for solving the technical problem of the present invention
A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: neatly putting the high nutritive cube of high 10cm-12cm in flat pallet container, alms bowl is built-in
Enter the Vermiculitum of 4cm-6cm, make Vermiculitum surfacing, pallet injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Mali pumilae subculture tissue cultured seedling of more than 40 days is transported in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 1.5cm-2.5cm, plucks base
Portion 2-4 sheet lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the individual plant seedling sheared is inserted ready flat pallet container by 2cm × 2 cm line space
In interior nutritive cube, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in nutrition
In the space relatively closed in alms bowl, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then at flat pallet
Container injects the high nutritional solution of taking root of 1cm-2cm, carries out root culture；
D, period management of taking root: take root the stage after culturing young plants cuttage, temperature indoor temperature is maintained between 20-30 DEG C, and humidity keeps
More than 80%；
E, seedling exercising period management: root culture, after 15 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off after being cultivated for 3-5 days the plastic sheeting on nutritive cube or nutritive cube is moved to ground cultivate, in greenhouse
70% sunshade net shelters from heat or light, and humidity is maintained at more than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is in the nutritive cube of soil and decomposed manure, waters permeable in time, makes nursery stock gradually adapt to open country growing environment, wait seedling long
During to 5cm-10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Nutritional solution of taking root in described step a and step c is for consisting of: containing ammonium nitrate in every liter of nutritional solution of taking root
NH4NO31650mg, potassium nitrate KNO31900mg, potassium dihydrogen phosphate KH2PO4170 mg, sulphuric acid U.S. MgSO4·7H2O370mg, chlorine
Change calcium CaCl22H2O440 mg, potassium iodide KI0.41mg, boric acid H3BO33.1mg, manganese sulfate MnSO4·4H2O11.1mg, sulphuric acid
Zinc ZnSO4·7H2O4.3mg, sodium molybdate Na2MoO4·2H2O0.125mg, copper sulfate CuSO4·5H2O0.0125mg, cobaltous chloride
CoCl2·6H2O 0.0125mg, indolebutyric acid IBA0.2mg.
Temperature sprayable decreasing temperature and increasing humidity time higher in described step d, or shelter from heat or light with 70% sunshade net, when wherein spraying, can be by
Mist is sprayed directly on on the plastic foil of nutritive cube, to keep moisture content in pallet.
In described step f, wherein the mass ratio of soil and decomposed manure is 1:0.3.
Described flat pallet container is along high 2cm-4cm.
The present invention simulates in trying Fructus Mali pumilae test tube Seedling bottle environment of taking root, and makes culturing young plants that relatively close, with nutritional solution
Substrate is allowed to take root, after progressively remove nutritional solution, plastic foil, be directly entered the substrate culture stage.The method incorporates group training
The process taken root in Seedling bottle and transplant, makes to take root in culturing young plants directly, transplanting medium outer at bottle, simplifies production link, fall
Low production cost, improves emergence rate.This technical method is simple, and production cost is low, and planting percent is high, is presented as straight on producing
The economic benefit connect.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated:
Take root nutritional solution for consisting of: containing ammonium nitrate NH in every liter of nutritional solution of taking root4NO31650mg, potassium nitrate
KNO31900mg, potassium dihydrogen phosphate KH2PO4170 mg, sulphuric acid U.S. MgSO4·7H2O370mg, calcium chloride CaCl22H2O440
Mg, potassium iodide KI0.41mg, boric acid H3BO33.1mg, manganese sulfate MnSO4·4H2O11.1mg, zinc sulfate ZnSO4·
7H2O4.3mg, sodium molybdate Na2MoO4·2H2O0.125mg, copper sulfate CuSO4·5H2O0.0125mg, cobaltous chloride CoCl2·6H2O
0.0125mg, indolebutyric acid IBA0.2mg.
Embodiment 1
A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: select the flat pallet container of the convenient carrying of size, flat pallet container along high 2cm,
Neatly put the high nutritive cube of high 12cm in flat pallet container, in nutritive cube, load the high Vermiculitum of 6cm, make Vermiculitum surface put down
Whole, the flat pallet container loading Vermiculitum injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Mali pumilae subculture tissue cultured seedling of more than 40 days is drawn in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 1.5cm, plucks base portion 2-4 sheet
Lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the group training individual plant seedling sheared is inserted ready flat pallet by 2cm × 2 cm line space
In nutritive cube in container, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in
In the space relatively closed in nutritive cube, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then flat
Pallet container injects the high nutritional solution of 2cm, carries out root culture；
D, period management of taking root: cuttage little seedling rooting stage, temperature indoor temperature be maintained between 20-30 DEG C, humidity be maintained at 80% with
On, sprayable decreasing temperature and increasing humidity when temperature is higher, during spraying, mist can be sprayed directly on on the plastic foil of nutritive cube, to keep flat
Moisture content in pallet container；
E, seedling exercising period management: root culture, after 15 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off the plastic sheeting on nutritive cube after being cultivated for 5 days, in greenhouse, 70% sunshade net shelters from heat or light, and humidity is maintained at
More than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is for soil: decomposed manure mass ratio is in the nutritive cube of 1:0.3, waters permeable in time, makes nursery stock gradually adapt to open country growth
Environment, when waiting seedling to grow to 5cm～10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Embodiment 2
A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: select the flat pallet container of the convenient carrying of size, flat pallet container along high 4cm,
Neatly put the high nutritive cube of high 10cm in flat pallet container, in nutritive cube, load the high Vermiculitum of 4cm, make Vermiculitum surface put down
Whole, the flat pallet container loading Vermiculitum injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Mali pumilae subculture tissue cultured seedling of more than 40 days is drawn in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 2.5cm, plucks base portion 2-4 sheet
Lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the group training individual plant seedling sheared is inserted ready flat pallet by 2cm × 2 cm line space
In nutritive cube in container, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in
In the space relatively closed in nutritive cube, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then flat
Pallet container injects the high nutritional solution of 1cm, carries out root culture；
D, period management of taking root: cuttage little seedling rooting stage, temperature indoor temperature be maintained between 20-30 DEG C, humidity be maintained at 80% with
On, sprayable decreasing temperature and increasing humidity when temperature is higher, during spraying, mist can be sprayed directly on on the plastic foil of nutritive cube, to keep flat
Moisture content in pallet container；
E, seedling exercising period management: root culture, after 15 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off the plastic sheeting on nutritive cube after being cultivated for 3 days, in greenhouse, 70% sunshade net shelters from heat or light, and humidity is maintained at
More than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is for soil: decomposed manure mass ratio is in the nutritive cube of 1:0.3, waters permeable in time, makes nursery stock gradually adapt to open country growth
Environment, when waiting seedling to grow to 5cm～10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Embodiment 3
A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling, including the clip of material of taking root, the preparation of facility of taking root, the joining of nutritional solution
Put, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: select the flat pallet container of the convenient carrying of size, flat pallet container along high 4cm,
Neatly put the high nutritive cube of high 10cm in flat pallet container, in nutritive cube, load the high Vermiculitum of 4cm, make Vermiculitum surface put down
Whole, the flat pallet container loading Vermiculitum injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Mali pumilae subculture tissue cultured seedling of more than 40 days is drawn in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 2.0cm, plucks base portion 2-4 sheet
Lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the group training individual plant seedling sheared is inserted ready flat pallet by 2cm × 2 cm line space
In nutritive cube in container, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in
In the space relatively closed in nutritive cube, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then flat
Pallet container injects the high nutritional solution of 1cm, carries out root culture；
D, period management of taking root: cuttage little seedling rooting stage, temperature indoor temperature be maintained between 20-30 DEG C, humidity be maintained at 80% with
On, shelter from heat or light with 70% sunshade net when temperature is higher；
E, seedling exercising period management: root culture, after 15 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, after being cultivated for 5 days, nutritive cube is moved to ground cultivate, in the greenhouse in continue 70% sunshade net shelter from heat or light, humidity holding
Manage more than 80%；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is for soil: decomposed manure mass ratio is in the nutritive cube of 1:0.3, waters permeable in time, makes nursery stock gradually adapt to open country growth
Environment, when waiting seedling to grow to 5cm-10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Claims (5)
1. the outside sprout-cultivating-bottle method of a Tissue-cultured apple seedling, it is characterised in that: include the take root clip of material, the standard of facility of taking root
Standby, the configuration of nutritional solution, the cuttage of culturing young plants, period management of taking root, seedling exercising period management, point plant period management step:
A, the preparation of outside sprout-cultivating-bottle facility: neatly putting the high nutritive cube of high 10cm-12cm in flat pallet container, alms bowl is built-in
Enter the Vermiculitum of 4cm-6cm, make Vermiculitum surfacing, pallet injects nutritional solution of taking root, and allows nutritional solution all be impregnated with Vermiculitum；
B, the clip of material of taking root: the expanding propagation Fructus Mali pumilae subculture tissue cultured seedling of more than 40 days is transported in greenhouse, with tweezers from culture medium
Middle taking-up plexi seedling, cuts base portion callus on operating board, is cut into the individual plant seedling of long 1.5cm-2.5cm, plucks base
Portion 2-4 sheet lobule, loads in container, with cutting with inserting；
C, culturing young plants cuttage: the individual plant seedling sheared is inserted ready flat pallet container by 2cm × 2 cm line space
In interior nutritive cube, after sticking with a flat pallet container, by White plastic film, nutritive cube mouth is covered, make seedling be placed in nutrition
In the space relatively closed in alms bowl, the flat pallet container sticking with seedling is neatly placed in heliogreenhouse, then at flat pallet
Container injects the high nutritional solution of taking root of 1cm-2cm, carries out root culture；
D, period management of taking root: take root the stage after culturing young plants cuttage, temperature indoor temperature is maintained between 20-30 DEG C, and humidity keeps
More than 80%；
E, seedling exercising period management: root culture, after 15 days, is observed situation of taking root, when seedling base portion bears fasciculate root, cleared out pallet
In moisture content, throw off after being cultivated for 3-5 days the plastic sheeting on nutritive cube or nutritive cube is moved to ground cultivate, in greenhouse
70% sunshade net shelters from heat or light, and humidity is maintained at more than 80% management；
F, point cultivation period management: when the little seedling leaf in nutritive cube turns green, thickening, during root system acceleration of growth, move into seedling individual plant
Substrate is in the nutritive cube of soil and decomposed manure, waters permeable in time, makes nursery stock gradually adapt to open country growing environment, wait seedling long
During to 5cm-10cm, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
The outside sprout-cultivating-bottle method of a kind of Tissue-cultured apple seedling the most according to claim 1, it is characterised in that: described step a and
Nutritional solution of taking root in step c is for consisting of: containing ammonium nitrate NH in every liter of nutritional solution of taking root4NO31650mg, potassium nitrate
KNO31900mg, potassium dihydrogen phosphate KH2PO4170 mg, sulphuric acid U.S. MgSO4·7H2O370mg, calcium chloride CaCl22H2O440
Mg, potassium iodide KI0.41mg, boric acid H3BO33.1mg, manganese sulfate MnSO4·4H2O11.1mg, zinc sulfate ZnSO4·
7H2O4.3mg, sodium molybdate Na2MoO4·2H2O0.125mg, copper sulfate CuSO4·5H2O0.0125mg, cobaltous chloride CoCl2·6H2O
0.0125mg, indolebutyric acid IBA0.2mg.
The outside sprout-cultivating-bottle method of a kind of Tissue-cultured apple seedling the most according to claim 1 and 2, it is characterised in that: described step d
Middle temperature is sprayable decreasing temperature and increasing humidity time higher, or shelters from heat or light with 70% sunshade net, when wherein spraying, mist can be sprayed directly on to nutritive cube
Plastic foil on, to keep moisture content in pallet.
The outside sprout-cultivating-bottle method of a kind of Tissue-cultured apple seedling the most according to claim 1 and 2, it is characterised in that: described step f
In wherein soil and the mass ratio of decomposed manure are 1:0.3.
The outside sprout-cultivating-bottle method of a kind of Tissue-cultured apple seedling the most according to claim 1 and 2, it is characterised in that: described flat
Pallet container is along high 2cm-4cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610641619.XA CN106212237B (en) | 2016-08-08 | 2016-08-08 | Ex-bottle rooting method for apple tissue culture seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610641619.XA CN106212237B (en) | 2016-08-08 | 2016-08-08 | Ex-bottle rooting method for apple tissue culture seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106212237A true CN106212237A (en) | 2016-12-14 |
| CN106212237B CN106212237B (en) | 2021-05-04 |
Family
ID=57547481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610641619.XA Active CN106212237B (en) | 2016-08-08 | 2016-08-08 | Ex-bottle rooting method for apple tissue culture seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106212237B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243756A (en) * | 2018-03-20 | 2018-07-06 | 山东农业大学 | A kind of Tissue-cultured apple seedling bottle grafts the micro-grafting method taken root outside |
| CN109863932A (en) * | 2018-10-28 | 2019-06-11 | 陕西海棠生态农林股份有限公司 | A kind of method of nutritive cube cultivation Tissue-cultured apple seedling |
| CN111149674A (en) * | 2020-02-28 | 2020-05-15 | 科稷达隆生物技术有限公司 | Culture method for improving transplanting survival rate of plant tissue culture seedlings |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103380730A (en) * | 2013-08-05 | 2013-11-06 | 江苏省农业科学院 | Tissue-culture rapid propagation method for pyrus betulaefolia bunge |
| CN103988762A (en) * | 2014-04-18 | 2014-08-20 | 天水市果树研究所 | Water culturing and transplanting method for apple tissue culture seedlings |
| CN104488726A (en) * | 2015-01-13 | 2015-04-08 | 湖南省农业生物资源利用研究所 | Outside-bottle rooting method of radix pseudostellariae test-tube plantlets |
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
| CN105075777A (en) * | 2015-08-24 | 2015-11-25 | 山东省烟台市农业科学研究院 | Blueberry moss seedling breeding method |
| CN105660391A (en) * | 2014-11-17 | 2016-06-15 | 孟三军 | Tissue culture breeding method for apple sapling |
-
2016
- 2016-08-08 CN CN201610641619.XA patent/CN106212237B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103380730A (en) * | 2013-08-05 | 2013-11-06 | 江苏省农业科学院 | Tissue-culture rapid propagation method for pyrus betulaefolia bunge |
| CN103988762A (en) * | 2014-04-18 | 2014-08-20 | 天水市果树研究所 | Water culturing and transplanting method for apple tissue culture seedlings |
| CN105660391A (en) * | 2014-11-17 | 2016-06-15 | 孟三军 | Tissue culture breeding method for apple sapling |
| CN104488726A (en) * | 2015-01-13 | 2015-04-08 | 湖南省农业生物资源利用研究所 | Outside-bottle rooting method of radix pseudostellariae test-tube plantlets |
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
| CN105075777A (en) * | 2015-08-24 | 2015-11-25 | 山东省烟台市农业科学研究院 | Blueberry moss seedling breeding method |
Non-Patent Citations (1)
| Title |
|---|
| 范志强等: "苹果砧木M9组培幼苗瓶外生根研究", 《山东林业科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243756A (en) * | 2018-03-20 | 2018-07-06 | 山东农业大学 | A kind of Tissue-cultured apple seedling bottle grafts the micro-grafting method taken root outside |
| CN108243756B (en) * | 2018-03-20 | 2020-04-14 | 山东农业大学 | Micro-grafting method for grafting and rooting outside apple tissue culture seedling bottle |
| CN109863932A (en) * | 2018-10-28 | 2019-06-11 | 陕西海棠生态农林股份有限公司 | A kind of method of nutritive cube cultivation Tissue-cultured apple seedling |
| CN111149674A (en) * | 2020-02-28 | 2020-05-15 | 科稷达隆生物技术有限公司 | Culture method for improving transplanting survival rate of plant tissue culture seedlings |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106212237B (en) | 2021-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102986445B (en) | Process for cutting thick branches of golden camellia | |
| CN106258958A (en) | A kind of outside sprout-cultivating-bottle method of Fructus Pruni pseudocerasi tissue cultured seedling | |
| CN103988762B (en) | A kind of water planting transplanting method of Tissue-cultured apple seedling | |
| CN104380944B (en) | Hongsenhuai sophora softwood cutting propagation method | |
| CN104012308B (en) | A kind of seedling-cultivating method of watermelon Long term cultivation | |
| CN106613961B (en) | A kind of breeding method of Chinese catalpa micropropagation | |
| CN104272954A (en) | Potted blueberry planting method | |
| CN106069543B (en) | A kind of Kiwi berry detoxication and tissue culture method for transplanting | |
| CN103583340A (en) | Gardenia multi-branch floating bed water planting cuttage method | |
| CN104335813A (en) | Cutting propagation method of acer truncatum | |
| CN103988764B (en) | The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening | |
| CN105123479A (en) | Water culture transplanting method for cherry tissue culture seedlings | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN106212237A (en) | A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling | |
| CN102613051A (en) | Method for plant rapid propagation through air culture | |
| CN102318546A (en) | Method for growing container seedlings of cork oak in winter | |
| CN103314862B (en) | A kind of method of efficient acquisition Chunlan detoxification seedling | |
| CN111264328A (en) | Cultivation method of small bougainvillea spectabilis pot culture | |
| CN103718969A (en) | Regenerated plant in-vitro culture method for Sirindhorn michelia figo | |
| CN105850441A (en) | Planting method of lemon bonsai | |
| CN103283504B (en) | Method for grafting pear polyploidy test-tube plantlet outside test tube | |
| CN103999772A (en) | In-vitro rapid propagation method of macromitrium cavaleriei card and ther | |
| CN104221725A (en) | Cultivation method for big and strong oil-tea camellia grafting seedlings | |
| CN103548543B (en) | Culture method for cuttage of melia azedarach seedlings | |
| CN103053347B (en) | Winter greenhouse grafting method for English hawthorn 'Scarlet' planted in Beijing area |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |