CN101233807A - Oriental China fir tissue culture seedling ex vitro rooting technique - Google Patents
Oriental China fir tissue culture seedling ex vitro rooting technique Download PDFInfo
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- CN101233807A CN101233807A CNA2008100341782A CN200810034178A CN101233807A CN 101233807 A CN101233807 A CN 101233807A CN A2008100341782 A CNA2008100341782 A CN A2008100341782A CN 200810034178 A CN200810034178 A CN 200810034178A CN 101233807 A CN101233807 A CN 101233807A
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Abstract
The invention relates to a technique for cultivating roots of tissue culture seedlings of oriental fir under outside a test tube, wherein non-root tissue culture seedlings of the oriental fir with height of 4-5cm and diameter of 2mm for a stem basal part are inserted into coarse sand of a cuttage seed bed. The seed bed is treated with disinfection in advance and 8-10cm of the coarse sand is spread on the seed bed; humidity is controlled by an interval spraying device. Air humidity is maintained to be more than 90 percent within 1-2 week(s) after cuttage, but no water drop can be formed on the leaves. Two weeks later, the humidity is maintained to be 60-80 percent so as to prevent the leaves from wilting; 40-60 days later, tissue culture seedlings with roots are moved to a nutrition pot with mixed matrix while the formula of the mixed-matrix is that soil: peat soil: prelim marble: sand is equal to 2 : 1 : 1 : 1. Two months later, the tissue culture seedlings of oriental fir are moved to the outside after new leaves grow out; after lignification of main stems, the tissue culture seedlings are moved to the field. The technique has the advantages that: the rooting process of inside a test tube is saved while the rooting process is moved to the outside, which reduces the cost of rooting period; by adopting the method of container seedling, survival rate in transplanting can be improved and reproductive speed is high without seasonal restrictions.
Description
Technical field
The present invention relates to a kind of oriental China fir tissue culture seedling ex vitro rooting technology.
Background technology
The east China fir is the crossbreed of Chinese cryptomeria and Taxodium mucronatum, has only a small amount of cultivation at present, can not be because of there being seed by a large amount of breedings of sowing, and breed with the method for cuttage and can be transplanted a cutting the restriction of quantity again.Therefore, in the Orient the prolific initial stage of China fir seedling, the method breeding seedling with the group training makes that breeding a large amount of seedlings in the short period of time becomes possibility.The tissue culture propagation seedling is compared with cottage propagation, and cost is higher relatively, and the technology among use the present invention is promptly taken root and hardening is finished synchronously, and reduction group is greatly trained the production cost in the stage of taking root.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of oriental China fir tissue culture seedling ex vitro rooting technology.
The present invention solves the problems of the technologies described above the technical scheme of being taked and realizes by implementing following technical proposals:
A kind of oriental China fir tissue culture seedling ex vitro rooting technology, earlier with highly about 4～5 centimetres, the east China fir unrooted tissue cultivating seedling of basal part of stem diameter more than 2 millimeters inserts in the rough sand in cuttage seedbed, and described seedbed through disinfecting, is covered with the rough sand of 8～10 cm thicks in advance above; With gap sprayer unit controlled humidity, 1～2 all interior air humiditys remain on more than 90% after the cuttage, but require blade can not form water droplet, and humidity remain on 60～80% after 2 weeks, here are yardstick to keep the nursery stock blade not wither; After 40～60 days, the tissue cultivating seedling immigration of having taken root is equipped with in the nutritive cube of mixed-matrix, the prescription of mixed-matrix is soil: peat soil: perlite: husky=2: 1: 1: 1, and the method rooting rate can reach 88%; After extracting young leaves out in two months, move to outdoor; Treat to transplant the land for growing field crops after the tissue cultivating seedling stem lignification.
On the basis of such scheme, the described unrooted tissue cultivating seedling incubation time in bottle that is used for cuttage was 5～6 weeks.
The described tissue cultivating seedling cuttage degree of depth is about 1 centimetre, and peels off the blade of basal part of stem, and cuttage seeding requires uprightly not tilt, and cutting density is 5 centimetres of 3 cm x.
With automatic backlash sprayer unit control air humidity, the little shed of seedbed top frame, and with film or sunshade net covering maintenance humidity, with 1000～1200 times wide-spectrum bactericide sprayed in whole seedbed weekly, and suitable ventilation, ventilative, prevent microbial growth.
Mixed-matrix is packed into and is placed in successively behind the nutritive cube on the seedbed that is covered with ground cloth.
Mixed-matrix must carry out fumigation before using, and the stifling medicament that uses is potassium permanganate (gram): formaldehyde (milliliter)=1: 2.
Outdoor, should spread ground cloth under the nutritive cube, avoid the tissue cultivating seedling root system to penetrate in the soil; Here move on to 1～2 week after outdoor, should keep certain humidity, should add a cover the sunshade net when sunlight is strong, be standard to guarantee that nursery stock does not wither.
The specification of described nutritive cube is 12 centimetres of bore * height=13 cm x.
Tissue cultivating seedling reaches lignified and specifically is characterized as outdoor: the stem height reach degree of lignification more than 2/3.
The present invention has at first realized the quick breeding of east China fir seedling, for the construction in China southeastern coast shelter belt has increased a kind of alternative seeds.Also provide reference simultaneously for the factorial seedling growth of realizing the conifer seeds that difficult breeding, difficulty are taken root.
Beneficial effect advantage of the present invention is:
1, with the seedling raising process of tissue cultivating seedling routine, promptly behind the tissue cultivating seedling strengthening seedling and rooting, carry out the booth hardening, transplant again and compare, the present invention has omitted this process of in vitro taking root, and the tissue cultivating seedling rooting process has been moved on to outdoor, has reduced the cost in the stage of taking root;
2, with the method for container nursery, root system is not destroyed during transplanting, has improved transplanting survival rate;
3, with the method for tissue culture propagation, reproduction speed is fast, and is not subjected to the restriction in season, has improved production efficiency greatly;
4, with the method for group training, the elite stand of high-quality is set up clone, can keep the merit of east China fir, as growth rate, tree-like or the like.
Specific implementation method
A kind of oriental China fir tissue culture seedling ex vitro rooting technology, at first the seedbed that cuttage is used is drenched with 0.1% liquor potassic permanganate water and is disinfected, spread the rough sand of 8～10 cm thicks then, after husky bed waters sufficient water, to in bottle, incubation time it be 5～6 weeks, highly about 4～5 centimetres, the east China fir unrooted tissue cultivating seedling of basal part of stem diameter more than 2 millimeters inserts in the rough sand in cuttage seedbed, the cuttage degree of depth is about 1 centimetre, seedling requires very to stand not tilt, the tissue cultivating seedling cutting density is 5 centimetres of 3 cm x, should note in the cuttage process preserving moisture.
After cuttage is intact, with film Zhi Haoxiao shed, and with automatic backlash sprayer unit controlled humidity, after cuttage 1～2 all in air humidity remain on more than 90%, and require blade can not form water droplet; Humidity remains on 60～80% after 2 weeks, here is yardstick to keep the nursery stock blade not wither, with 1000～1200 times wide-spectrum bactericide sprayed in whole seedbed weekly, and suitable ventilation, ventilative, prevent microbial growth.
After 40～60 days, the tissue cultivating seedling of having taken root moved into be equipped with in the nutritive cube of mixed-matrix, the nutritive cube size of dress mixed-matrix is, 12 centimetres of bores * height=13 cm x, and the prescription of mixed-matrix is:
Soil: peat soil: perlite: husky=2: 1: 1: 1;
Must carry out fumigation before mixed-matrix uses, the stifling medicament that uses is potassium permanganate (gram): formaldehyde (milliliter)=1: 2, nutritive cube install be placed in successively behind the mixed-matrix be covered with on the seedbed of cloth, nutritive cube in turn against, but be not close to.
After tissue cultivating seedling was extracted young leaves out in 2 months, move to outdoor.Outdoor, should spread ground cloth under the nutritive cube, avoid the tissue cultivating seedling root system to penetrate in the soil; Here move on to 1～2 week after outdoor, should keep certain humidity, should add a cover the sunshade net when sunlight is strong, be standard to guarantee that nursery stock does not wither, and 2 Zhou Houying control moisture gradually, impel the nursery stock lignification.
Treat tissue cultivating seedling stem height reach lignification more than 2/3 after can transplant the land for growing field crops.
Claims (9)
1, a kind of oriental China fir tissue culture seedling ex vitro rooting technology, earlier with highly about 4～5 centimetres, the east China fir unrooted tissue cultivating seedling of basal part of stem diameter more than 2 millimeters inserts in the rough sand in cuttage seedbed, and described seedbed through disinfecting, is covered with the rough sand of 8～10 cm thicks in advance above; With gap sprayer unit controlled humidity, 1～2 all interior air humiditys remain on more than 90% after the cuttage, but require blade can not form water droplet, and humidity remain on 60～80% after 2 weeks, here are yardstick to keep the nursery stock blade not wither; After 40～60 days, the tissue cultivating seedling immigration of having taken root is equipped with in the nutritive cube of mixed-matrix, the prescription of mixed-matrix is soil: peat soil: perlite: husky=2: 1: 1: behind 1, two month extraction young leaves, move to outdoor; Treat to transplant the land for growing field crops after the tissue cultivating seedling stem lignification.
2, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1 is characterized in that: the described unrooted tissue cultivating seedling incubation time in bottle that is used for cuttage was 5～6 weeks.
3, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1 is characterized in that: the described tissue cultivating seedling cuttage degree of depth is about 1 centimetre, and peels off the blade of basal part of stem, and cuttage seeding requires uprightly not tilt, and cutting density is 5 centimetres of 3 cm x.
4, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1, it is characterized in that: with automatic backlash sprayer unit control air humidity, the little shed of seedbed top frame, and cover with film or sunshade net and to keep humidity, with 1000～1200 times wide-spectrum bactericide sprayed in whole seedbed weekly, and suitable ventilation, ventilative, prevent microbial growth.
5, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1 is characterized in that: mixed-matrix is packed into and is placed in successively behind the nutritive cube on the seedbed that is covered with ground cloth.
6, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 5 is characterized in that: mixed-matrix must carry out fumigation before using, and the stifling medicament that uses is potassium permanganate (gram): formaldehyde (milliliter)=1: 2.
7, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1 is characterized in that: outdoor, should spread ground cloth under the nutritive cube, avoid the tissue cultivating seedling root system to penetrate in the soil; Here move on to 1～2 week after outdoor, should keep certain humidity, should add a cover the sunshade net when sunlight is strong, be standard to guarantee that nursery stock does not wither.
8, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1 is characterized in that: the specification of described nutritive cube is 12 centimetres of bore * height=13 cm x.
9, oriental China fir tissue culture seedling ex vitro rooting technology according to claim 1 is characterized in that: tissue cultivating seedling reaches lignified and specifically is characterized as outdoor: the stem height reach degree of lignification more than 2/3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100341782A CN101233807A (en) | 2008-03-03 | 2008-03-03 | Oriental China fir tissue culture seedling ex vitro rooting technique |
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| CNA2008100341782A CN101233807A (en) | 2008-03-03 | 2008-03-03 | Oriental China fir tissue culture seedling ex vitro rooting technique |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524012A (en) * | 2012-02-17 | 2012-07-04 | 天水市果树研究所 | Exercising and transplanting method for fruit tree tissue culture seedlings |
| CN102696480A (en) * | 2012-05-07 | 2012-10-03 | 广西壮族自治区林业科学研究院 | Method for culturing aseptic oil tea buds in test tube by using sand matrix |
| CN103081703A (en) * | 2013-02-19 | 2013-05-08 | 河南省木本饲料工程技术研究中心 | Tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method |
| CN104885842A (en) * | 2015-04-28 | 2015-09-09 | 江苏省中国科学院植物研究所 | Method for cutting and breeding Taxus baccata |
| CN106380238A (en) * | 2016-08-22 | 2017-02-08 | 广西壮族自治区林业科学研究院 | A cultivating method for improving the root system architecture of a containerized seedling of keteleeria cyclolepis flous |
| CN109997537A (en) * | 2019-05-21 | 2019-07-12 | 黑龙江伊蓝生物科技有限公司 | The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling |
| CN113994819A (en) * | 2021-09-18 | 2022-02-01 | 江苏省中国科学院植物研究所 | Tissue culture and cuttage combined rapid propagation method for sequoia |
| CN114946625A (en) * | 2022-06-09 | 2022-08-30 | 安徽科技学院 | Method for rooting outside test tube of pomegranate tissue culture seedling |
| CN115104473A (en) * | 2022-06-02 | 2022-09-27 | 江苏省中国科学院植物研究所 | Sowing and seedling raising method for hybrid Mexico larch |
-
2008
- 2008-03-03 CN CNA2008100341782A patent/CN101233807A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524012A (en) * | 2012-02-17 | 2012-07-04 | 天水市果树研究所 | Exercising and transplanting method for fruit tree tissue culture seedlings |
| CN102524012B (en) * | 2012-02-17 | 2013-02-27 | 天水市果树研究所 | Exercising and transplanting method for fruit tree tissue culture seedlings |
| CN102696480A (en) * | 2012-05-07 | 2012-10-03 | 广西壮族自治区林业科学研究院 | Method for culturing aseptic oil tea buds in test tube by using sand matrix |
| CN103081703A (en) * | 2013-02-19 | 2013-05-08 | 河南省木本饲料工程技术研究中心 | Tetraploid robinia pseudoacacia tissue cultured seedling out-of-bottle cuttage rooting method |
| CN104885842A (en) * | 2015-04-28 | 2015-09-09 | 江苏省中国科学院植物研究所 | Method for cutting and breeding Taxus baccata |
| CN106380238A (en) * | 2016-08-22 | 2017-02-08 | 广西壮族自治区林业科学研究院 | A cultivating method for improving the root system architecture of a containerized seedling of keteleeria cyclolepis flous |
| CN106380238B (en) * | 2016-08-22 | 2019-11-12 | 广西壮族自治区林业科学研究院 | A kind of breeding method improving Jiangnan keteleeria fortunei container seedling root system configuration |
| CN109997537A (en) * | 2019-05-21 | 2019-07-12 | 黑龙江伊蓝生物科技有限公司 | The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling |
| CN113994819A (en) * | 2021-09-18 | 2022-02-01 | 江苏省中国科学院植物研究所 | Tissue culture and cuttage combined rapid propagation method for sequoia |
| CN115104473A (en) * | 2022-06-02 | 2022-09-27 | 江苏省中国科学院植物研究所 | Sowing and seedling raising method for hybrid Mexico larch |
| CN114946625A (en) * | 2022-06-09 | 2022-08-30 | 安徽科技学院 | Method for rooting outside test tube of pomegranate tissue culture seedling |
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