CN105359802A - Reproduction method for American vetches - Google Patents

Reproduction method for American vetches Download PDF

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Publication number
CN105359802A
CN105359802A CN201510825268.3A CN201510825268A CN105359802A CN 105359802 A CN105359802 A CN 105359802A CN 201510825268 A CN201510825268 A CN 201510825268A CN 105359802 A CN105359802 A CN 105359802A
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China
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vetch
seed
stem
american
propagation method
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黄勇
杨金龙
王高富
周鹏
殷素会
王琳
王海威
张翔宇
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SICHUAN ANIMAL HUSBANDRY SCIENCE RESEARCH INSTITUTE
Chongqing Academy of Animal Sciences
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SICHUAN ANIMAL HUSBANDRY SCIENCE RESEARCH INSTITUTE
Chongqing Academy of Animal Sciences
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Priority to CN201510825268.3A priority Critical patent/CN105359802A/en
Publication of CN105359802A publication Critical patent/CN105359802A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention belongs to the technical field of agricultural cultivation , and more particularly relates to a reproduction method for American vetches. The reproduction method provided by the present invention comprises: selecting American vetche seed stems that grow fast and have shorter internodes, and trimming branches to forming cuttings on the same day; soaking the cuttings into a seed-soaking solution, wherein the seed-soaking solution is an aqueous solution containing 100-500 mg/L of sodium naphthalene-1-acetate, 100-500 mg/L of potassium indoxyl butyrate and 100-500 mg/L of dipotassium hydrogen phosphate; and inserting the soaked American vetch seed stem cuttings into a substrate. According to the reproduction method provided by the present invention, the American vetch seed stems are inserted after being soaked with the seed-soaking solution, so that the seedling quality of the American vetches can be effectively improved, cell division of the American vetch seed stems can be promoted, adventitious roots are induced to be formed, the root activity is increased, the growth rate of new branches is fastened, the survival rate is increased, and the dry matter accumulation amount is increased, thereby improving the seeding reproduction speed and increasing the economic benefits.

Description

The propagation method of U.S.'s vetch
Technical field
The invention belongs to agricultural cultivation biological technical field, particularly the propagation method of a kind of U.S. vetch.
Background technology
U.S.'s vetch (ViciasepiumLinn), vegetative kingdom, Dicotyledoneae, pulse family, perennial herb, spends more, and is a kind of good nectariferous plant, with all herbal medicine.Summer adopts, and dries or using fresh herb.Root can be eaten something rare, and boils rear taste better.Seed oil-containing.Leaf and the medicinal effect having heat-clearing, eliminating inflammation and expelling toxin of flowers and fruits.Plant is beautiful, pattern is gorgeous, can do ornamental flower.Occur that comparatively early at this moment branch is very few squaring period, stem branch is short, delicate soft, and its nutrient component thick protein is up to 26.9%, and now grass yield is also high.After the beginning of spring, along with temperature raises, plant starts swift and violent growth, and April 14 entered pod bearing period, and plant is up to 83.5cm, though cauline leaf be still green, at this moment kowtow that to cut grass yield high, nutrient component reduces, and raw fiber increase, thick protein is then reduced to 18.55%.From plant branch situation, during autumn sowing, 30 – 40 can be reached at the branch amount of productive phase, and at pod bearing period branch only about 20 when sowing in spring.Thus optimum receive the period of cutting should in pod bearing period, cut also comparatively congener early about 20 days in period from receipts, this promotes in Rice Region of South China, to stubble contradiction before and after alleviation is very favourable.The contradiction that still can solve sowing and herbage is promoted in south China Dryland; Therefore, vetch is expected to cultivate into the green manure new varieties promoted in southern farmland.Vetch cauline leaf calcic, phosphorus are abundanter, raise, ensiling and modulation hay as green grass or young crops.The plantation of U.S.'s vetch both can meet the needs of improvement of the ecological environment, promoted the important measures of sustainable development of animal husbandry especially.
U.S.'s vetch main product, in the ground such as the U.S., Canada, also has introducing and planting on the ground such as NORTHWEST CHINA, southwestern each provinces and regions.In recent years, by the impact of the pulling of domestic and international market, national development of the West Regions, conceding the land to forestry and grass and improvement of the ecological environment etc., China's nectariferous plant development speed is very fast, and excellent seed (seedling) is the necessary material base of bee-keeping development.
Under field conditions (factors), sprout time is long, and germination rate is low, and seedling breeding is restricted for U.S.'s vetch seed, have impact on the spread plantation of U.S. vetch.
Although have research and the report of the propagation method of many plants at present, the propagation method at present about U.S.'s vetch does not also have research and patent disclosure.
Summary of the invention
In view of this, the object of the present invention is to provide the propagation method of a kind of U.S. vetch (ViciasepiumLinn), in propagation method provided by the invention, the kind stem of seed soaking solution to U.S.'s vetch is adopted to soak rear cuttage, effectively can improve the seedling quality of U.S.'s vetch: U.S.'s vetch kind stalk cell division can be promoted, induced synthesis adventive root, increase improving activity of root system, accelerate new branch growth rate, improve survival rate, increase ripe diameter etc. of planting stem, and then improve sapling multiplication speed, increase economic benefit.For achieving the above object, technical scheme of the present invention is as follows:
The propagation method of U.S.'s vetch, comprises the following steps:
1) preparation of cutting: choose U.S.'s vetch kind stem that robust growth, internode are shorter, pruning branch the same day is cutting;
2) immersion of cutting: by step 1) described cutting is immersed in seed soaking solution, and described seed soaking solution is the aqueous solution comprising the a-Nafusaku of 100-500mg/L, 100-500mg/L butyric acid potassium and 100-500mg/L dipotassium hydrogen phosphate;
3) cuttage of U.S.'s vetch: get step 2) soak after cutting cuttage enter in matrix.
The preparation of seed soaking solution: a-Nafusaku, butyric acid potassium and dipotassium hydrogen phosphate are dissolved in the ethanol of 10 times amount, obtains solution mother liquor, gets mother liquor and pour in the water of 10-200 times amount, and limit bevelling stirs, and obtains soaking solution.
Preferred as one, seed soaking solution preparation method is, a-Nafusaku, butyric acid potassium, dipotassium hydrogen phosphate are dissolved in the ethanol of 50 times amount, obtain solution mother liquor, getting seed soaking ethanolic solution pours in the water of described seed soaking ethanolic solution 66.67-200 times amount, need limit bevelling to stir, obtain seed soaking solution.
The kind stem of seed soaking solution to U.S.'s vetch is adopted to soak rear cuttage, effectively can improve the seedling quality of U.S.'s vetch: U.S.'s vetch kind stalk cell division can be promoted, induced synthesis adventive root, increase improving activity of root system, accelerate new branch growth rate, improve survival rate, increase ripe diameter etc. of planting stem, increase dry-matter accumulation amount, and then improve sapling multiplication speed, increase economic benefit.
A-Nafusaku is auxins plant growth regulator, is the plant growth regulator of spectrum, promotes cell division and tissue differentiation, for expanding of Fruit and block root stem tuber.The formation of induction root and adventive root, takes root and seed root of hair for accelerating cuttage branch.Can also be used for flower and fruit protecting, improve output, strengthen anti-adversity ability etc., be the plant growth conditioner of a kind of wide spectrum, efficient, low toxicity.Butyric acid potassium is a kind of growth-promoting root class plant growth regulator, is mainly used in rooting of cuttings agent, and induction crop forms adventive root, has long-lasting and narrow spectrum feature, and concentrate on growing point position, promote cell division, induced synthesis adventive root, shows as root many, root is straight, and root is thick, and root hair is many.Soluble in water, active high, good stability, use safety.
Butyric acid potassium is a kind of growth-promoting root class plant growth regulator.Induction crop forms adventive root, via foliage spray, dips in the modes such as root, is passed to and enter plant corpus, and concentrate on growing point position by positions such as blade seeds, and promote cell division, induced synthesis adventive root, shows as root many, and root is straight, and root is thick, and root hair is many.Soluble in water, specific activity heteroauxin is high, slowly can decompose, store, molecular structure stabilized under dark conditions under high light.Can act on plant whole body and respectively grow vigorous position, as root, tender shoots, fruit is cell division to single-minded treatment sites strong expression, growth promoting effects.Butyric acid potassium has long-lasting and narrow spectrum feature, can promote new root, and induction root body is formed, and promotes cutting Adventitious root initiation, and good stability, use safety is good short long agent of taking root.
Potassium dihydrogen phosphate is a kind of nutrient chemical, can be used for preparing medium, and plant growth needs phosphorus and potassium, and potassium dihydrogen phosphate, as buffer, can provide in the medium and increase K and P element.Containing the nutriment required for explant growth in medium, it is the basis that in tissue cultures, explant is depended on for existence and development.In each stage of external tissue cultures, minimal medium is all most important factor, therefore selects suitable medium to be the key factor of tissue cultures success or not.
Further, step 1) described cutting stem is long is 10-40cm.Cutting is certain stem length is plant quality to ensure.
Further, step 1) described cutting stem have 1-3 joint.
Further, step 2) time of described immersion is 1-24 hour.In immersion process to U.S.'s vetch kind stem, the concentration of soak solution and soak time have material impact to U.S.'s vetch cottage propagation.The soak time that in preferred enforcement, the concentration of different soak solution is corresponding different.
Preferred as one, when soak solution is the aqueous solution comprising the a-Nafusaku of 250mg/L, 250mg/L butyric acid potassium and 250mg/L dipotassium hydrogen phosphate, soak 12 hours cutting experiment the bests.
Preferred as one, when soak solution is the aqueous solution comprising 350mg/La-Nafusaku, 350mg/L butyric acid potassium and 350mg/L dipotassium hydrogen phosphate, soak 8 hours cutting experiment the bests.
Preferred as one, when soak solution is the aqueous solution comprising 400mg/La-Nafusaku, 400mg/L butyric acid potassium and 400mg/L dipotassium hydrogen phosphate, soak 6 hours cutting experiment the bests.
Preferred as one, when soak solution is the aqueous solution comprising 500mg/La-Nafusaku, 500mg/L butyric acid potassium and 500mg/L dipotassium hydrogen phosphate, soak 4 hours cutting experiment the bests.
Further, step 3) the vetch kind stem cutting of the described U.S. becomes 30-45 ° of angle with ground.In order to improve plantation income, cuttage is that U.S.'s vetch kind stem and ground are angled.
Further, step 3) degree of depth of cuttage of described cutting is 1-2cm.
Further, step 3) spacing in the rows of cuttage of described cutting is 20-30cm.
Further, step 3) line-spacing of cuttage of described cutting is 25-35cm.
Further, step 3) described matrix comprises sandy soil.
Further, the propagation method of U.S.'s vetch also comprises step: 4) transplant after cuttage 10-30 days.
Beneficial effect of the present invention is: the propagation method that the invention provides a kind of U.S. vetch.Propagation method of the present invention adopts the kind stem of seed soaking solution to U.S.'s vetch to soak rear cuttage, effectively can improve the seedling quality of U.S.'s vetch: U.S.'s vetch kind stalk cell division can be promoted, induced synthesis adventive root, increase improving activity of root system, accelerate new branch growth rate, improve survival rate, increase ripe diameter etc. of planting stem, increase dry-matter accumulation amount, and then improve sapling multiplication speed, increase economic benefit.This method is sent out propagation method and is significantly regulated U.S.'s vetch cottage propagation growth, the long 0.5-3cm of the longest root of U.S.'s vetch can be increased, increase radical 1-10 bar, increase the long 1-15cm of new branch, increase survival rate 50-80%, improve improving activity of root system 50-500 μ gTPF.g-1FW.h-1, increase the ripe diameter 0.05-0.5mm planting stem.
Embodiment
Below with reference to embodiment, the present invention is described in detail.In order to make object of the present invention, technical scheme and advantage clearly understand, the present invention is further elaborated in conjunction with the embodiments.The experimental technique of unreceipted actual conditions in preferred embodiment, usually conveniently condition.Illustrated embodiment is to be described content of the present invention better, but is not that content of the present invention is only limitted to illustrated embodiment.So those of ordinary skill in the art carry out nonessential improvement and adjustment according to foregoing invention content to embodiment, still belong to protection scope of the present invention.
The propagation method of U.S.'s vetch
Material
Cutting: choose U.S.'s vetch kind stem that robust growth, internode are shorter, pruning branch the same day is cutting;
Seed soaking solution: a-Nafusaku, butyric acid potassium and dipotassium hydrogen phosphate are dissolved in the ethanol of 10 times amount, obtains solution mother liquor, gets mother liquor and pour in the water of 10-200 times amount, and limit bevelling stirs, and obtains soaking solution.
Embodiment 1
Experimental group: the healthy and strong stipes (being cut into 30cm section with scraper, every section of tool 3 joints) choosing U.S.'s vetch stem middle and lower part even thickness is trophosome.Line-spacing 33cm, spacing in the rows 26cm, every cave plants 1 strain, and density is 1.16 × 105 strains/hm 2.In cuttage on May 10, by line-spacing trench digging, dark 10cm, puts kind of a stem by spacing in the rows, makes the inclination at 45 ° of kind of stem and ground.Base fertilizer is made, 1500kg/ mu with cow dung.Test adopts dual factors randomised block design, and a-Nafusaku, butyric acid potassium, dipotassium hydrogen phosphate solution concentration arrange 4 levels, and each composition is respectively 100mg/L, 200mg/L, 300mg/L and 400mg/L.Wherein, the mass volume ratio of each composition, ethanol, water is 1: 50: 2500-10000.Each for equivalent composition 1g is total to each composition of 4g to be dissolved in the ethanol of 50mL, obtain seed soaking ethanolic solution, get seed soaking ethanolic solution to pour into respectively in the water of 10000mL, 5000mL, 3333mL, 2500mL (ethanolic solution 50-200 times amount of namely soaking seed), limit bevelling stirs, and obtains seed soaking solution.Plant stem soak time and establish 3 levels, be respectively 4h, 8h and 12h.Other the same Production of Large Fields of cultivation management measure, after cuttage, 35d carries out sampling and measuring.
Control group: U.S.'s vetch kind stem soaks without seed soaking solution, other the same experimental group of cultivation management measure.
Result of the test shows: U.S.'s vetch kind stem seed soaking solution soaks survival rate and reaches as high as 100%, and control group is only up to 10%.
Result shows, compared with the control group without seed soaking solution process, the survival rate utilizing each composition to be the process of 100mg/L-300mg/L seed soaking solution in experimental group, improving activity of root system generally improve, new branch is long to be increased, diameter increases thick, and output when planting stem maturation generally improves, and difference has significance.In experimental group, be that 100mg/L seed soaking solution soaks kind of a stem with each composition, when soaking 4h, survival rate, improving activity of root system are all significantly higher than contrast, new branch is long, diameter and and output with contrast there was no significant difference; When soaking 8h, the long and output of survival rate, improving activity of root system, new branch is all significantly higher than contrast, diameter with contrast there was no significant difference; When soaking 12h, survival rate, new branch length, improving activity of root system and output are all significantly higher than contrast in pole, and diameter is significantly higher than contrast.Be that 200mg/L seed soaking solution soaks kind of a stem with each composition, when soaking 4h, the long and output of survival rate, improving activity of root system, new branch is all significantly higher than contrast, diameter with contrast there was no significant difference; When soaking 8h, survival rate, new branch length, improving activity of root system and output are all significantly higher than contrast in pole, and diameter is significantly higher than contrast; When soak 12h time, survival rate, improving activity of root system are significantly higher than contrast, all the other indexs with contrast there was no significant difference.Be that 300mg/L seed soaking solution soaks kind of a stem with each composition, when soaking 4h, survival rate, new branch are long, improving activity of root system and output is all significantly higher than contrast in pole, diameter is significantly higher than contrast; When soak 8h time, survival rate, improving activity of root system and output are significantly higher than contrast, all the other indexs with contrast there was no significant difference; When soaking 12h, survival rate, improving activity of root system, new branch are long significantly lower than contrast, all the other indexs with contrast there was no significant difference.When being 400mg/L seed soaking solution immersion kind stem with each composition, each index all lower than contrast, and reduces with the prolongation of soak time.Therefore, being that the seed soaking solution of 100-300mg/L soaks cuttage after U.S. vetch kind stem by adopting each composition, significantly regulating U.S.'s vetch cottage propagation growth.
Embodiment 2
Experimental group: the healthy and strong stipes (being cut into 40cm section with scraper, every section of tool 3 joints) choosing U.S.'s vetch stem middle and lower part even thickness is trophosome.Line-spacing 33cm, spacing in the rows 26cm, every cave plants 1 strain, and density is 1.16 × 105 strains/hm 2.In cuttage on May 10, by line-spacing trench digging, dark 10cm, puts kind of a stem by spacing in the rows, makes kind of stem become 30 ° with ground and tilts.Nitrogen-phosphorus-potassium compound fertilizer is used as base fertilizer according to the amount of application of 10kg/ mu; In described nitrogen-phosphorus-potassium compound fertilizer, the mass percentage of nitrogen is 16%, the mass percentage of phosphorus element is 15%, the mass percentage of potassium element is 14%.Test adopts dual factors randomised block design, and each constituent concentration of seed soaking solution arranges 4 levels, is respectively 100mg/L, 200mg/L, 300mg/L and 400mg/L.Wherein, the mass volume ratio of each composition, ethanol, water is 1: 50: 2500-10000.Each for equivalent composition 1g is total to 4g to be dissolved in the ethanol of 50mL, obtain seed soaking ethanolic solution, get seed soaking ethanolic solution to pour into respectively in the water of 10000mL, 5000mL, 3333mL, 2500mL (ethanolic solution 50-200 times amount of namely soaking seed), limit bevelling stirs, and obtains seed soaking solution.Plant stem soak time and establish 3 levels, be respectively 4h, 8h and 12h.Other the same Production of Large Fields of cultivation management measure, after cuttage, 35d carries out sampling and measuring.
Control group: U.S.'s vetch kind stem soaks without seed soaking solution, other the same experimental group of cultivation management measure.
Result of the test shows, compared with the control group without seed soaking solution process, utilizes the survival rate of 100mg/L-300mg/L seed soaking solution process in experimental group, improving activity of root system generally improves, new branch is long to be increased, diameter increases thick, and output when planting stem maturation generally improves, and difference has significance.In experimental group, soak kind of a stem with 100mg/L seed soaking solution, when soaking 4h, the long and output of survival rate, improving activity of root system, new branch is all significantly higher than contrast, diameter with contrast there was no significant difference; When soaking 8h, the long and output of survival rate, improving activity of root system, new branch is all significantly higher than contrast, diameter with contrast there was no significant difference; When soaking 12h, survival rate, new branch length, improving activity of root system and output are all significantly higher than contrast in pole, and diameter is significantly higher than contrast.Soak kind of a stem with each composition 200mg/kg seed soaking solution, when soaking 4h, survival rate, improving activity of root system, new branch is long is all significantly higher than contrast, diameter and output with contrast there was no significant difference; When soaking 8h, survival rate, new branch length, improving activity of root system and output are all significantly higher than contrast in pole, and diameter is significantly higher than contrast; When soak 12h time, improving activity of root system and output are significantly higher than contrast, all the other indexs with contrast there was no significant difference.Soak kind of a stem with 300mg/L seed soaking solution, when soaking 4h, survival rate, new branch length, improving activity of root system and output are all significantly higher than contrast in pole, and diameter is significantly higher than contrast; When soak 8h time, survival rate, improving activity of root system are significantly higher than contrast, and output pole is significantly higher than contrast, all the other indexs with contrast there was no significant difference; When soaking 12h, survival rate, improving activity of root system, new branch are long significantly lower than contrast, all the other indexs with contrast there was no significant difference.When being 400mg/L seed soaking solution immersion kind stem with each composition, each index all lower than contrast, and reduces with the prolongation of soak time.Therefore, being that the seed soaking solution of 100-300mg/L soaks cuttage after U.S. vetch kind stem 4-12h by adopting each composition, significantly regulating U.S.'s vetch cottage propagation growth.
Embodiment 3
Experimental group: the healthy and strong stipes (being cut into 10cm section with scraper, every section of tool 1 joint) choosing U.S.'s vetch stem middle and lower part even thickness is trophosome.Be 5cm in cuttage on March 10 at diameter, in the basin of high 10cm, make kind of stem become 40 ° with ground and tilt.Put into green house, make day temperature 25 DEG C, cultivate at nocturnal temperature 20 DEG C.Test adopts dual factors randomized complete-block design, and seed soaking solution concentration arranges 5 levels, is respectively 100mg/L, 200mg/L, 300mg/L, 400mg/L and 500mg/L.Wherein, the mass volume ratio of each composition, ethanol, water is 1: 50: 2000-10000.Each for equivalent composition 1g is total to 4g to be dissolved in the ethanol of 50mL, obtain seed soaking ethanolic solution, getting seed soaking ethanolic solution pours in the water of 10000mL, 5000mL, 3333mL, 2500mL, 2000mL (ethanolic solution 40-200 times amount of namely soaking seed) respectively, limit bevelling stirs, and obtains seed soaking solution.Plant stem soak time and establish 3 levels, be respectively 4h, 8h and 12h.Nitrogen-phosphorus-potassium compound fertilizer is used as base fertilizer according to the amount of application of 15kg/ mu; In described nitrogen-phosphorus-potassium compound fertilizer, the mass percentage of nitrogen is 14%, the mass percentage of phosphorus element is 16%, the mass percentage of potassium element is 15%.Other the same Production of Large Fields of cultivation management measure, after cuttage, 15d carries out sampling and measuring.
Control group: U.S.'s vetch kind stem soaks without seed soaking solution, other the same experimental group of cultivation management measure.
Result of the test shows, compared with the control group without seed soaking solution process, long, the longest root length of the new branch utilizing each composition to be the process of 100mg/L-300mg/L seed soaking solution in experimental group and radical generally improve.In experimental group, be that 100mg/L seed soaking solution soaks kind of a stem with each composition, when soaking 4h, long, the longest root length of new branch and radical are all higher than contrast, but there was no significant difference; When soaking 8h, the long and the longest root of new branch is long is significantly higher than contrast, radical with contrast there was no significant difference; When soaking 12h, new branch is long is significantly higher than contrast with the long pole of the longest root, and radical is significantly higher than contrast.Soak kind of a stem with each composition 200mg/L seed soaking solution, when soaking 4h, long, the longest root length of new branch and radical are all significantly higher than contrast; When soaking 8h, the long and the longest root length of new branch and radical are all significantly higher than contrast in pole; When soaking 12h, long, the longest root length of new branch and radical are all higher than contrast, but there was no significant difference.Be that 300mg/L seed soaking solution soaks kind of a stem with each composition, when soaking 4h and 8h respectively, the long and radical of long, the longest root of new branch is all higher than contrast, but there was no significant difference; When soaking 12h, the long and the longest root of new branch is long significantly lower than contrast, radical with contrast there was no significant difference.When planting stem with the seed soaking solution immersion of 400mg/L and 500mg/L, each index all lower than contrast, and reduces with the raising of soaking concentration and the prolongation of soak time.Therefore, being that the seed soaking solution of 100-300mg/L soaks cuttage after U.S. vetch kind stem 4-12h by adopting each composition, significantly regulating U.S.'s vetch cottage propagation growth.
In above result of the test, the method is by cuttage after employing seed soaking solution immersion U.S. vetch kind stem, the vetch cottage propagation growth of the remarkable adjustment U.S., the long 0.5-3cm of the longest root of U.S.'s vetch can be increased, increase radical 1-10 bar, increase the long 1-15cm of new branch, increase survival rate 50-80%, improve improving activity of root system 50-500 μ gTPF.g-1FW.h-1, increase the ripe diameter 0.05-0.5mm planting stem.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.

Claims (10)

1. the propagation method of U.S.'s vetch, is characterized in that, comprises the following steps:
1) preparation of cutting: choose U.S.'s vetch kind stem that robust growth, internode are shorter, pruning branch the same day is cutting;
2) immersion of cutting: by step 1) described cutting is immersed in seed soaking solution, and described seed soaking solution is the aqueous solution comprising the a-Nafusaku of 100-500mg/L, 100-500mg/L butyric acid potassium and 100-500mg/L dipotassium hydrogen phosphate;
3) cuttage of U.S.'s vetch: get step 2) in soak after cutting cuttage enter in matrix.
2. propagation method according to claim 1, is characterized in that, step 1) described cutting stem is long is 10-40cm.
3. propagation method according to claim 1, is characterized in that, step 1) described cutting stem have 1-3 joint.
4. propagation method according to claim 1, is characterized in that, step 2) time of described immersion is 1-24 hour.
5. propagation method according to claim 1, is characterized in that, step 3) described cutting becomes 30-45 ° of angle with ground.
6. propagation method according to claim 1, is characterized in that, step 3) degree of depth of the cuttage of described cutting is 1-2cm.
7. propagation method according to claim 1, is characterized in that, step 3) spacing in the rows of the cuttage of described cutting is 20-30cm.
8. propagation method according to claim 1, is characterized in that, step 3) line-spacing of the cuttage of described cutting is 25-35cm.
9. propagation method according to claim 1, is characterized in that, step 3) described matrix comprises sandy soil.
10. propagation method according to claim 1, is characterized in that, also comprises step:
4) transplant after cuttage 10-30 days.
CN201510825268.3A 2015-11-24 2015-11-24 Reproduction method for American vetches Pending CN105359802A (en)

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Cited By (3)

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CN106070326A (en) * 2016-06-11 2016-11-09 徐伟明 A kind of special powder and cottage method thereof of taking root of Huanghua Pear
CN109169289A (en) * 2018-11-06 2019-01-11 湖南省林业科学院 A kind of crape myrtle tissue culture proliferation seedling rooting medicament and method
CN115280933A (en) * 2022-07-05 2022-11-04 云南省农业科学院甘蔗研究所 Method for preparing single-bud sugarcane seeds

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Application publication date: 20160302