CN103444382A - Cultivation technology for Taxus media - Google Patents

Cultivation technology for Taxus media Download PDF

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Publication number
CN103444382A
CN103444382A CN 201210170170 CN201210170170A CN103444382A CN 103444382 A CN103444382 A CN 103444382A CN 201210170170 CN201210170170 CN 201210170170 CN 201210170170 A CN201210170170 A CN 201210170170A CN 103444382 A CN103444382 A CN 103444382A
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CN
China
Prior art keywords
branch
taxus
nutrient solution
soil
cuttage
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Pending
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CN 201210170170
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Chinese (zh)
Inventor
许永丰
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ZHEJIANG HUNDRED YEARS CHINESE YEW BIOTECHNOLOGY Co Ltd
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ZHEJIANG HUNDRED YEARS CHINESE YEW BIOTECHNOLOGY Co Ltd
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Priority to CN 201210170170 priority Critical patent/CN103444382A/en
Publication of CN103444382A publication Critical patent/CN103444382A/en
Pending legal-status Critical Current

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Abstract

The invention relates to a cultivation technology for Taxus media. The cultivation technology comprises branch selection, soaking of branches in nutrient solutions and cuttage cultivation in soil, to be specific, cutting Taxus branches into a plurality of sections, performing cuttage treatment on each section of Taxus branch to enable the Taxus branches to take root and sprout by using the regeneration capacity, wherein the length of each cutting is 10-15cm, and the bottom diameter of each cutting is 3-5cm; bundling up every 30 cuttings cut according to the specifications into one small bundle, and enabling lower cuts to be even and upper cuts to be uneven; soaking bundled branches in the nutrient solutions for 48 hours. According to the cultivation technology for the Taxus media, easy-to-survive cuttage branch selection standards of the Taxus media are achieved, and the selected formula of soaking cuttage branches in the nutrient solutions and the environmental condition of transplant soil matrix are quite suitable for survival of the Taxus. The survival rate of the Taxus can reach above 90% through the cultivation technology for the Taxus media.

Description

A kind of taxus media culture technology
Technical field
The present invention relates to field of planting, it is specifically related to a kind of taxus media culture technology.
Background technology
The Taxus shallow rooted plant, its main root is not obvious, lateral root is flourishing, is the endangered natural rare plants for anticancer of generally acknowledging in the world, is through the ancient seeds that carry over of quaternary glacier, the history of existing 2,500,000 years on earth.Because growth of taxol speed is slow under field conditions (factors), regeneration capacity is poor, so, for a long time, also there is no to form the Chinese yew raw material forest base that large rule are touched in world wide.China has classified it as one-level Precious, Rare, Endangered protective plant, and the United Nations also prohibites felling.The seedling percent that wherein the Taxus x media conventional art of China is cultivated is low, and kind is impure, affects the content of taxol, has restricted growth rate.Meanwhile, because reproductive survival rate is low, cause seedling cost high, directly affected productivity effect and the economic benefit of yew industry.So be badly in need of now improving.
Summary of the invention
For the problems referred to above, the objective of the invention is to propose a kind of survival rate that improves Taxus x media, guarantee the taxus media culture technology of high-purity Japanese yew content.
A kind of taxus media culture technology, it is a kind of cottage breeding technology, comprises the choosing branch, soaks nutrient solution and soil cottage breeding,
Described choosing branch technology is as follows: the Chinese yew branch is divided into to the dry section of promise, utilizes its regeneration capacity, each section Chinese yew branch carried out to the cuttage processing, allow the Chinese yew branch root; The choosing branch will be considered following factor: should select the treelet below 5 years; Branch should be selected its middle part; Select to give birth to then hardwood cuttings according to the branch development situation, cutting length is 10-15cm, and cutting footpath, the end is 3-5mm; The cutting sheared by above specification, every 30 are bundled into wisp, accomplish that lower cut is neat, and upper cut is uneven;
The branch of accomplishing fluently bundle is immersed in nutrient solution to 48 hours, and the formula of described nutrient solution is as follows:
Sodium hydroxide 1.00-3.00g/L
Beta-naphthylacetic acid 9.00-15.00g/L
Heteroauxin 10.00-18.00g/L
The branch that soaks nutrient solution is carried out to cottage breeding in the soil matrix prepared, described soil matrix is: Zhu Yan and composite soil humous, Zhu Yan and ratio humous are 1: 2, add in right amount peanut skin 30-80g/kg, should guarantee that the nitrogen content in soil is 200-220mg/kg (ammonium state) simultaneously, phosphorus content is 90-100mg/kg, and potassium content is 130-140mg/kg, and the ph value of soil is 5.0-8.0; The temperature of cuttage is 18 ℃-27 ℃, and relative moisture is 60-80%.
Hydroxide in described nutrient solution is metal hydroxides, as: sodium hydroxide and potassium hydroxide.
The formula of described nutrient solution is as follows:
Sodium hydroxide 1.00-2.00g/L
Beta-naphthylacetic acid 10.00-12.00g/L
Heteroauxin 12.00-16.00g/L
The formula of described nutrient solution is as follows:
Sodium hydroxide 2.00-3.00g/L
Beta-naphthylacetic acid 10.00-15.00g/L
Heteroauxin 10.00-16.00g/L
The present invention, owing to taking above technical scheme, has the following advantages:
A kind of taxus media culture technology of the present invention has obtained the cuttage choosing branch standard that Taxus x media the most easily survives, and selected cuttage branch soaks the formula of nutrient solution and the environmental condition of rice transplanting soil matrix, is applicable to very much surviving of Chinese yew.Utilize the technology of the present invention to carry out the survival rate that can reach more than 90% of breeding of Chinese yew.
Embodiment
The first embodiment of a kind of taxus media culture technology of the present invention is as follows:
It is a kind of cottage breeding technology, comprise the choosing branch, soak nutrient solution and soil cottage breeding, described choosing branch technology is as follows: the Chinese yew branch is divided into to the dry section of promise, utilizes its regeneration capacity, each section Chinese yew branch carried out to the cuttage processing, allow the Chinese yew branch root; The choosing branch will be considered following factor: should select the treelet below 5 years; Branch should be selected wherein middle part; Select to give birth to then hardwood cuttings according to the branch development situation, cutting length is 10cm, and cutting footpath, the end is 3mm; The cutting sheared by above specification, every 30 are bundled into wisp, accomplish that lower cut is neat, and upper cut is uneven;
The branch of accomplishing fluently bundle is immersed in nutrient solution to 48 hours, and the formula of described nutrient solution is as follows:
Sodium hydroxide 1.00g/L
Beta-naphthylacetic acid 10.00g/L
Heteroauxin 15.00g/L
The branch that soaks nutrient solution is carried out to cottage breeding in the soil matrix prepared, described soil matrix is: Zhu Yan and composite soil humous, Zhu Yan and ratio humous are 1: 2, add in right amount peanut skin 80g/kg, should guarantee that the nitrogen content in soil is 220mg/kg (ammonium state) simultaneously, phosphorus content is 90mg/kg, and potassium content is 135mg/kg, and the ph value of soil is 6.0; The temperature of cuttage is 20 ℃, and relative moisture is 60%.
The second embodiment of a kind of taxus media culture technology of the present invention is as follows:
It is a kind of cottage breeding technology, comprise the choosing branch, soak nutrient solution and soil cottage breeding, described choosing branch technology is as follows: the Chinese yew branch is divided into to the dry section of promise, utilizes its regeneration capacity, each section Chinese yew branch carried out to the cuttage processing, allow the Chinese yew branch root; The choosing branch will be considered following factor: should select the treelet below 5 years; Branch should be selected its middle part; Select to give birth to then hardwood cuttings according to the branch development situation, cutting length is 15cm, and cutting footpath, the end is 5mm; The cutting sheared by above specification, every 30 are bundled into wisp, accomplish that lower cut is neat, and upper cut is uneven;
The branch of accomplishing fluently bundle is immersed in nutrient solution to 48 hours, and the formula of described nutrient solution is as follows:
Sodium hydroxide 2.00g/L
Beta-naphthylacetic acid 12.00g/L
Heteroauxin 12.00g/L
The branch that soaks nutrient solution is carried out to cottage breeding in the soil matrix prepared, described soil matrix is: Zhu Yan and composite soil humous, Zhu Yan and ratio humous are 1: 2, add in right amount peanut skin 50g/kg, should guarantee that the nitrogen content in soil is 210mg/kg (ammonium state) simultaneously, phosphorus content is 100mg/kg, and potassium content is 140mg/kg, and the ph value of soil is 8.0; The temperature of cuttage is 27 ℃, and relative moisture is 80%.
The 3rd embodiment of a kind of taxus media culture technology of the present invention is as follows:
It is a kind of cottage breeding technology, comprise the choosing branch, soak nutrient solution and soil cottage breeding, described choosing branch technology is as follows: the Chinese yew branch is divided into to the dry section of promise, utilizes its regeneration capacity, each section Chinese yew branch carried out to the cuttage processing, allow the Chinese yew branch root; The choosing branch will be considered following factor: should select the treelet below 5 years; Branch should be selected its middle part; Select to give birth to then hardwood cuttings according to the branch development situation, cutting length is 12cm, and cutting footpath, the end is 4mm; The cutting sheared by above specification, every 30 are bundled into wisp, accomplish that lower cut is neat, and upper cut is uneven;
The branch of accomplishing fluently bundle is immersed in nutrient solution to 48 hours, and the formula of described nutrient solution is as follows:
Sodium hydroxide 2.00g/L
Beta-naphthylacetic acid 15.00g/L
Heteroauxin 10.00g/L
The branch that soaks nutrient solution is carried out to cottage breeding in the soil matrix prepared, described soil matrix is: Zhu Yan and composite soil humous, Zhu Yan and ratio humous are 1: 2, add in right amount peanut skin 70g/kg, should guarantee that the nitrogen content in soil is 215mg/kg (ammonium state) simultaneously, phosphorus content is 90mg/kg, and potassium content is 135mg/kg, and the ph value of soil is 6.5; The temperature of cuttage is 22 ℃, and relative moisture is 65%.
The 4th embodiment of a kind of taxus media culture technology of the present invention is as follows:
It is a kind of cottage breeding technology, comprise the choosing branch, soak nutrient solution and soil cottage breeding, described choosing branch technology is as follows: the Chinese yew branch is divided into to the dry section of promise, utilizes its regeneration capacity, each section Chinese yew branch carried out to the cuttage processing, allow the Chinese yew branch root; The choosing branch will be considered following factor: should select the treelet below 5 years; Branch should be selected its middle part; Select to give birth to then hardwood cuttings according to the branch development situation, cutting length is 15cm, and cutting footpath, the end is 3mm; The cutting sheared by above specification, every 30 are bundled into wisp, accomplish that lower cut is neat, and upper cut is uneven;
The branch of accomplishing fluently bundle is immersed in nutrient solution to 48 hours, and the formula of described nutrient solution is as follows:
Sodium hydroxide 3.00g/L
Beta-naphthylacetic acid 10.00g/L
Heteroauxin 16.00g/L
The branch that soaks nutrient solution is carried out to cottage breeding in the soil matrix prepared, described soil matrix is: Zhu Yan and composite soil humous, Zhu Yan and ratio humous are 1: 2, add in right amount peanut skin 40g/kg, should guarantee that the nitrogen content in soil is 200mg/kg (ammonium state) simultaneously, phosphorus content is 100mg/kg, and potassium content is 130mg/kg, and the ph value of soil is 7.5; The temperature of cuttage is 19 ℃, and relative moisture is 65%.
The present invention only illustrates explanation with above-described embodiment; the structure of each parts, setting position and connection thereof all can change to some extent; on the basis of technical solution of the present invention; all improvement of indivedual parts being carried out according to the principle of the invention and equivalents, all should not get rid of outside protection scope of the present invention.

Claims (4)

1. a taxus media culture technology, it is a kind of cottage breeding technology, comprises the choosing branch, soaks nutrient solution and soil cottage breeding, it is characterized in that:
Described choosing branch technology is as follows: the Chinese yew branch is divided into to the dry section of promise, utilizes its regeneration capacity, each section Chinese yew branch carried out to the cuttage processing, allow the Chinese yew branch root; The choosing branch will be considered following factor: should select the treelet below 5 years; Branch should be selected its middle part; Select to give birth to then hardwood cuttings according to the branch development situation, cutting length is 10-15cm, and cutting footpath, the end is 3-5mm; The cutting sheared by above specification, every 30 are bundled into wisp, accomplish that lower cut is neat, and upper cut is uneven;
The branch of accomplishing fluently bundle is immersed in nutrient solution to 48 hours, and the formula of described nutrient solution is as follows:
Sodium hydroxide 1.00-3.00g/L
Beta-naphthylacetic acid 9.00-15.00g/L
Heteroauxin 10.00-18.00g/L
The branch that soaks nutrient solution is carried out to cottage breeding in the soil matrix prepared, described soil matrix is: Zhu Yan and composite soil humous, Zhu Yan and ratio humous are 1: 2, add in right amount peanut skin 30-80g/kg, should guarantee that the nitrogen content in soil is 200-220mg/kg (ammonium state) simultaneously, phosphorus content is 90-100mg/kg, and potassium content is 130-140mg/kg, and the ph value of soil is 5.0-8.0; The temperature of cuttage is 18 ℃-27 ℃, and relative moisture is 60-80%.
2. a kind of taxus media culture technology as claimed in claim 1, it is characterized in that: the hydroxide in described nutrient solution is metal hydroxides, as: sodium hydroxide and potassium hydroxide.
3. a kind of taxus media culture technology as claimed in claim 2, it is characterized in that: the formula of described nutrient solution is as follows:
Sodium hydroxide 1.00-2.00g/L
Beta-naphthylacetic acid 10.00-12.00g/L
Heteroauxin 12.00-16.00g/L
4. a kind of taxus media culture technology as claimed in claim 2, it is characterized in that: the formula of described nutrient solution is as follows:
Sodium hydroxide 2.00-3.00g/L
Beta-naphthylacetic acid 10.00-15.00g/L
Heteroauxin 10.00-16.00g/L
CN 201210170170 2012-05-28 2012-05-28 Cultivation technology for Taxus media Pending CN103444382A (en)

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CN 201210170170 CN103444382A (en) 2012-05-28 2012-05-28 Cultivation technology for Taxus media

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Application Number Priority Date Filing Date Title
CN 201210170170 CN103444382A (en) 2012-05-28 2012-05-28 Cultivation technology for Taxus media

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103947437A (en) * 2014-05-13 2014-07-30 兴文县康永红豆杉生物科技有限责任公司 Taxus X media non-test tube rapid propagation method
CN104585249A (en) * 2015-02-06 2015-05-06 江西万茂科技有限公司 Solution capable of promoting rooting of taxus media
CN105248228A (en) * 2015-11-13 2016-01-20 周福进 Taxus media cultivation method
CN105359802A (en) * 2015-11-24 2016-03-02 重庆市畜牧科学院 Reproduction method for American vetches
CN106069496A (en) * 2016-06-20 2016-11-09 普定县持恒林业科技发展有限公司 A kind of Yew cuttage method for culturing seedlings
CN106258504A (en) * 2015-06-11 2017-01-04 北京林业大学 Tree-shaped tatarian dogwood Cultivating techniques
CN114271141A (en) * 2021-12-24 2022-04-05 中国农业科学院都市农业研究所 Technology for inducing aerial rooting and breeding of taxus chinensis by utilizing agrobacterium rhizogenes

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103947437A (en) * 2014-05-13 2014-07-30 兴文县康永红豆杉生物科技有限责任公司 Taxus X media non-test tube rapid propagation method
CN104585249A (en) * 2015-02-06 2015-05-06 江西万茂科技有限公司 Solution capable of promoting rooting of taxus media
CN106258504A (en) * 2015-06-11 2017-01-04 北京林业大学 Tree-shaped tatarian dogwood Cultivating techniques
CN105248228A (en) * 2015-11-13 2016-01-20 周福进 Taxus media cultivation method
CN105359802A (en) * 2015-11-24 2016-03-02 重庆市畜牧科学院 Reproduction method for American vetches
CN106069496A (en) * 2016-06-20 2016-11-09 普定县持恒林业科技发展有限公司 A kind of Yew cuttage method for culturing seedlings
CN114271141A (en) * 2021-12-24 2022-04-05 中国农业科学院都市农业研究所 Technology for inducing aerial rooting and breeding of taxus chinensis by utilizing agrobacterium rhizogenes
CN114271141B (en) * 2021-12-24 2023-02-07 中国农业科学院都市农业研究所 Technology for inducing aerial rooting and breeding of taxus chinensis by utilizing agrobacterium rhizogenes

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Application publication date: 20131218