CN104054501B - A kind of Phoebe bournei cuttage breeding method - Google Patents
A kind of Phoebe bournei cuttage breeding method Download PDFInfo
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Abstract
本发明公开了一种闽楠扦插繁殖方法，包括母株培育、截干促萌、插穗选择与扦插、母株管理和扦插苗管理工序，其特征在于：将优良无性系苗木种植于装有基质的育苗器内培育采穗母株，于母株基部截断苗干并喷施激素促进萌芽，选择优质穗条经生根促进剂处理后扦插于荫棚内，喷施混合营养液，获得闽楠优良无性系扦插苗。本方法能够完全保持闽楠优树的优良性状，提高闽楠根系对营养的吸收能力，培养出高品质的闽楠枝条作插穗，繁殖系数和扦插成活率都比较高，成本低廉、推广容易，为闽楠种质资源保存和优良无性系苗木推广造林提供一种有效、可靠的方法，具有较好的社会效益和经济效益。The invention discloses a method for cutting and propagating Nannan fujian, which comprises the steps of mother plant cultivation, stem cutting to promote germination, cutting ear selection and cutting, mother plant management and cutting seedling management, and is characterized in that: good clone seedlings are planted in a substrate Cultivate the ear-picking mother plant in a seedling raising device, cut off the stem of the seedling at the base of the mother plant and spray hormones to promote germination, select high-quality ear strips and treat them with rooting promoters, cut them in the shade shed, and spray mixed nutrient solution to obtain fine-quality Fujian Nan. Clonal cuttings. The method can fully maintain the excellent character of the excellent tree of Phoebe chinensis, improve the absorption capacity of the root system of Phoebe chinensis, and cultivate high-quality branches of Phoebe nanzia for cuttings, the propagation coefficient and cutting survival rate are relatively high, the cost is low, and the popularization is easy. It provides an effective and reliable method for the preservation of Nannan germplasm resources and the promotion of afforestation of fine clone seedlings, and has good social and economic benefits.
Description
技术领域 technical field
本发明属于植物无性繁殖技术领域，涉及一种闽楠扦插繁殖方法。 The invention belongs to the technical field of plant asexual propagation, and relates to a cutting propagation method of nannus.
背景技术 Background technique
闽楠(Phoebe bournei)为樟科楠属常绿乔木，是我国Ⅱ级濒危植物，现仅零星分布于我国的湖北西部、浙江南部、福建、江西、湖南、广东、广西北部和贵州东部等海拔 200 ～ 1 000 m 的山地常绿阔叶林中。闽楠是我国特有的园林绿化树种和优良珍贵用材树种，但是由于天然林少，种子产量低，不能满足人工造林对苗木的需求。而通过无性繁殖，能够解决种子不足和遗传分化大的问题，为闽楠资源保存和开发利用开辟新的途径。曲芬霞(闽楠组培快繁技术研究，林业实用技术，2010，(11)：7-9.)通过组织培养的方法繁殖土沉香已经取得成功，但是组织培养生产条件和技术要求比较高，生产成本高，目前尚未能大面积推广，而扦插繁殖技术容易掌握，生产条件要求低，一般苗圃就能进行生产。在国内，关于闽楠扦插繁殖技术研究报道有谢庆宏等(闽楠嫩枝扦插繁殖技术研究，湖南林业科技，2011，38(6)：43-45.)，雷凌菁等(闽楠扦插繁殖技术研究，福建林业科技，2006，33(4)：104-106.)，申展等(不同因素对闽楠嫩枝扦插生根的影响，中南林业科技大学学报，2013，33(1)：63-67.)，苏村水(闽楠扦插繁殖技术研究，安徽农学通报，2011，17(13)：26-28.)，这些研究都是用实生苗作母株，不能保证完全保持母树的优良品质，且没有关于母株的培育方法、穗条的采方法以及采穗后对母株的管理技术，扦插成活率极不稳定。目前没有关于闽楠扦插繁殖方法的发明专利。本发明是选择用优树的组织进行组织培养所得的优良无性系苗木作采穗母株，种植在容器里，能完全保持母树的优良品质，母株管护和穗条采集方便易行，繁殖系数高，扦插成活率较高。 Phoebe bournei (Phoebe bournei) is an evergreen tree of the genus Phoebe in Lauraceae. It is a second-class endangered plant in my country. It is only scattered in western Hubei, southern Zhejiang, Fujian, Jiangxi, Hunan, Guangdong, northern Guangxi and eastern Guizhou in China. 200-1 000 m in mountainous evergreen broad-leaved forests. Nannan is a unique landscaping tree species and an excellent and precious timber tree species in China. However, due to the lack of natural forests and low seed yield, it cannot meet the needs of artificial afforestation for seedlings. And through asexual reproduction, it can solve the problems of insufficient seeds and large genetic differentiation, and open up a new way for the preservation, development and utilization of Minnan resources. Qu Fenxia (Research on Tissue Culture Rapid Propagation Technology of Minnan, Forestry Practical Technology, 2010, (11): 7-9.) has achieved success in propagating Agarwood by tissue culture, but the production conditions and technical requirements of tissue culture are relatively high , the production cost is high, and it has not been promoted in a large area at present, but the cutting propagation technology is easy to master, and the production conditions are low, and general nurseries can produce it. In China, there are Xie Qinghong et al. (Research on Cuttage Propagation Technology of Minnan Twigs, Hunan Forestry Science and Technology, 2011, 38(6): 43-45.), Lei Lingjing et al. Technical research, Fujian Forestry Science and Technology, 2006, 33(4): 104-106.), Shen Zhan et al. (The influence of different factors on the rooting of Nannan twig cuttings, Journal of Central South University of Forestry and Technology, 2013, 33(1): 63 -67.), Su Cunshui (Research on Cutting Propagation Technology of Nannan, Anhui Agricultural Science Bulletin, 2011, 17(13): 26-28.), these studies are all using seedlings as mother plants, and cannot guarantee the complete maintenance of mother trees Good quality, and there is no breeding method for the mother plant, picking method for the cuttings, and management techniques for the mother plant after picking, so the survival rate of the cuttings is extremely unstable. At present, there is no invention patent about the cutting propagation method of Fujian Nan. The present invention selects excellent clone seedlings obtained by tissue culture from the tissues of excellent trees as ear-picking mother plants, and plants them in containers to fully maintain the excellent quality of the mother trees. The coefficient is high, and the survival rate of cuttings is high.
发明内容 Contents of the invention
本发明的目的是针对闽楠扦插繁殖的不足，旨在提供一种闽楠扦插繁殖方法。 The object of the present invention is to aim at the deficiency of the cutting propagation of Fujian nan, and aims to provide a cutting propagation method of Fujian nan.
本发明的目的是通过以下技术方案实现的： The purpose of the present invention is achieved through the following technical solutions:
一种闽楠扦插繁殖方法，包括母株培育、截干促萌、插穗选择与扦插、母株管理和扦插苗管理工序，其特征在于：将优良无性系苗木种植于装有基质的育苗器内培育采穗母株，于母株基部截断苗干并喷施激素促进萌芽，选择优质穗条经生根促进剂处理后扦插于荫棚内，喷施混合营养液，获得闽楠优良无性系扦插苗，包括以下步骤： A method for cutting and propagating Phoebe fukiensis, including mother plant cultivation, stem cutting to promote germination, cutting selection and cutting, mother plant management and cutting seedling management procedures, characterized in that: good clone seedlings are planted in a seedling raising device equipped with a substrate Cultivate ear-picking mother plants, cut off the stems of the seedlings at the base of the mother plants and spray hormones to promote germination, select high-quality ear cuttings and treat them with rooting promoters, cut them in the shade shed, spray mixed nutrient solution, and obtain excellent clone cutting seedlings of Nannan , including the following steps:
(1)母株培育：将优良无性系苗木种植于装有母株基质的母株培育器内，种植母株时覆土低于原苗木的容器杯口，并按株距×行距为30～40 cm×50～60 cm摆放，培育采穗母株； (1) Mother plant cultivation: plant excellent clone seedlings in a mother plant incubator equipped with a mother plant substrate. When planting a mother plant, the covering soil is lower than the container cup of the original seedlings, and the distance between plants and rows is 30-40 cm. × 50-60 cm placed, cultivate ear-picking mother plants;
(2)截干促萌：于母株基部截断主干，并追肥和喷施激素促进萌芽； (2) Cut off the stem to promote germination: cut off the trunk at the base of the mother plant, and topdress and spray hormones to promote germination;
(3)插穗选择与扦插：采集优质穗条，用生根促进剂处理插穗后，按常规方法将插穗扦插到荫棚内装有扦插基质的容器里； (3) Cutting selection and cutting: collect high-quality cuttings, after processing the cuttings with a rooting accelerator, the cuttings are cut into the container with the cutting substrate in the shade shed according to the conventional method;
(4)母株管理：采集穗条后，进行追肥； (4) mother plant management: after gathering the ear bar, carry out topdressing;
(5)扦插苗管理：用塑料薄膜覆盖扦插床，每隔5～7天掀开薄膜，视基质干湿情况按常规方法浇水和喷施混合营养液，待插穗生根稳定后，除掉薄膜。 (5) Cutting seedling management: Cover the cutting bed with plastic film, lift the film every 5 to 7 days, water and spray mixed nutrient solution according to the conventional method according to the dryness and humidity of the substrate, and remove the film after the cuttings take root and stabilize .
以上步骤（1）母株培育中所述的母株基质其组分和重量份数为：黄心土或森林表土10份、木糠或椰糠10份、经过熟化的农家肥或泥碳土0.1～0.3份。 The components and parts by weight of the mother plant substrate described in the above step (1) mother plant cultivation are: 10 parts of yellow heart soil or forest topsoil, 10 parts of wood bran or coconut bran, 0.1 to 0.1 parts of matured farmyard manure or peat soil 0.3 parts.
以上步骤（1）母株培育中所述的母株培育器是选用口和底的直径为25～30 cm、高为40～50 cm的控根育苗器。 The mother plant incubator described in the above step (1) mother plant cultivation is a root-controlling seedling cultivation device with a mouth and bottom diameter of 25-30 cm and a height of 40-50 cm.
以上步骤（2）截干促萌中所述的截断主干其方法是：对地径达到0.5～0.8 cm的母株，于基部25～30 cm处截断主干。 The method of cutting off the trunk as described in the above step (2) to cut off the stem and promote germination is: cut off the trunk at the base of 25 to 30 cm for the mother plant with a ground diameter of 0.5 to 0.8 cm.
以上步骤（2）截干促萌中所述的追肥和喷施激素其方法是：按常规方法对每株母株追施由北京惠民达科技发展中心生产的精制有机肥5～10 g + 复合肥(N:P:K=15:15:15) 5～10 g，浇透水，并按常规方法喷施质量浓度为20～50 mg.L-1的6-糠氨基嘌呤(6KT)。 The method of topdressing and spraying hormones described in the above step (2) cutting stems and promoting germination is: topdressing each mother plant with 5-10 g of refined organic fertilizer produced by Beijing Huiminda Science and Technology Development Center according to the conventional method + Compound fertilizer (N:P:K=15:15:15) 5-10 g, pour water thoroughly, and spray 6-furfurylaminopurine (6KT) with a mass concentration of 20-50 mg.L -1 according to conventional methods.
以上步骤（3）插穗选择与扦插中所述的优质穗条是于每年1～3月采集母株主干上萌生未抽新梢的二级侧枝，把侧枝按常规方法剪成长度为10～12 cm的插穗。 The high-quality scion described in the above step (3) cutting selection and cutting is to collect the secondary side branches that have not sprouted new shoots from the main trunk of the mother plant from January to March every year, and cut the side branches into lengths of 10 to 12 according to conventional methods. cm cuttings.
以上步骤（3）插穗选择与扦插中所述的扦插基质其组分和重量份数为：黄心土70份、椰糠29份和蛭石1份。 The components and parts by weight of the cutting substrate described in the above step (3) selection of cuttings and cutting are: 70 parts of yellow heart soil, 29 parts of coconut peat and 1 part of vermiculite.
以上步骤（4）母株管理中所述的追肥其方法是：采集完插穗后，每株母株则追施由北京惠民达科技发展中心生产的精制有机肥5～10 g + 复合肥(N:P:K=15:15:15) 5～10 g。 The method of topdressing in the above step (4) mother plant management is: after collecting the cuttings, each mother plant is topdressed with 5-10 g of refined organic fertilizer produced by Beijing Huiminda Science and Technology Development Center + compound fertilizer ( N:P:K=15:15:15) 5～10 g.
以上步骤（5）扦插苗管理中所述的喷施混合营养液是由含有质量浓度为0.2～0.3 %多肽尿素 + 0.2～0.3 %硫酸亚铁组成的混合营养液。 The spraying mixed nutrient solution described in the above step (5) management of cutting seedlings is a mixed nutrient solution composed of 0.2-0.3% polypeptide urea + 0.2-0.3% ferrous sulfate.
本发明相对于现有的技术具有以下优点： Compared with the prior art, the present invention has the following advantages:
1.本发明一种闽楠扦插繁殖方法，选择用优树的组织进行组织培养所得的优良无性系苗木作扦插母株，能够完全保持优树的优良性状，培育出的扦插苗是优良无性系苗，而育苗成本远远低于组织培养，可作为优良无性系苗木推广造林的主要育苗方法。 1. A kind of cutting propagation method of Phoebe fukiensis of the present invention, selects the fine clone seedling stock that carries out tissue culture gained with the tissue of excellent tree as cuttage mother plant, can fully keep the excellent character of excellent tree, and the cutting seedling cultivated is excellent clone Seedlings, and the cost of raising seedlings is far lower than tissue culture, and can be used as the main seedling raising method for promoting afforestation of fine clone seedlings.
2.本发明一种闽楠扦插繁殖方法，使用控根育苗器种植母株，克服了常规容器育苗带来根缠绕的缺陷，使母株形成大量短而粗的侧根，提高根系对营养的吸收能力，促进母株萌芽和萌芽枝的生长；并由于种植母株时覆土低于原来苗木的容器杯口，有利于从基部萌发新枝，提高繁殖系数。 2. A method for cutting and propagating Phoebe fukiensis of the present invention, using a root-controlling seedling device to plant the mother plant, which overcomes the defect of root entanglement caused by conventional container seedling cultivation, makes the mother plant form a large number of short and thick lateral roots, and improves the absorption of nutrients by the root system ability to promote the germination of the mother plant and the growth of sprouting branches; and because the covering soil is lower than the cup of the original seedling when the mother plant is planted, it is beneficial to germinate new branches from the base and increase the reproduction coefficient.
3.本发明一种闽楠扦插繁殖方法，在母株截干后喷施6-糠氨基嘌呤，可促进芽的萌发，提高繁殖系数；在扦插以后喷施多肽尿素+硫酸亚铁混合溶液，可有效促进插穗的光合作用和营养元素的转化，从而促进插穗生根，扦插成活率在94 ％左右，提高扦插成活率。 3. A kind of Fujian Nan cutting propagation method of the present invention, spray 6-furfuryl aminopurine after mother plant is cut off, can promote the germination of bud, improve reproduction coefficient; After cutting, spray polypeptide urea+ferrous sulfate mixed solution, It can effectively promote the photosynthesis of cuttings and the transformation of nutrient elements, thereby promoting the rooting of cuttings, the survival rate of cuttings is about 94%, and the survival rate of cuttings is improved.
具体实施方式 detailed description
通过下面给出的具体实施例，可以进一步清楚地了解本发明，但它们不是对本发明的限定。 Through the specific examples given below, the present invention can be further clearly understood, but they are not limitations of the present invention.
实施例Example 11 ：:
将优良无性系苗木种植于装有母株基质的母株培育器内，母株基质其组分和重量份数为：黄心土10份、椰糠10份、泥碳土0.2份，母株培育器是选用口和底的直径为30 cm、高为40 cm的控根育苗器。种植母株时覆土低于原苗木的容器杯口，并按株距×行距为40 cm×50 cm摆放，培育采穗母株。对地径达到0.5 cm的母株，于基部30 cm处截断主干。按常规方法对每株母株追施由北京惠民达科技发展中心生产的精制有机肥5 g + 复合肥(N:P:K=15:15:15) 10 g，浇透水，并按常规方法喷施质量浓度为30 mg.L-1的6-糠氨基嘌呤(6KT)。1月采集母株主干上萌生未抽新梢的的二级侧枝，把侧枝按常规方法剪成长度为10 cm的插穗。用生根促进剂处理插穗后，按常规方法将插穗扦插到荫棚内装有扦插基质的容器里，扦插基质其组分和重量份数为：黄心土70份、椰糠29份、蛭石1份。用塑料薄膜覆盖扦插床，每隔6天掀开薄膜，视基质干湿情况按常规方法浇水和喷施由含有质量浓度为0.2 %多肽尿素 + 0.3 %硫酸亚铁组成的混合营养液，待插穗生根稳定后，除掉薄膜。采集完插穗后，每株母株则追施由北京惠民达科技发展中心生产的精制有机肥10 g+复合肥(N:P:K=15:15:15) 5 g。平均每株母株得插穗10条，扦插成活率91.7 %。 The good clone seedlings are planted in the mother plant incubator equipped with the mother plant substrate. It is a root-controlling seedling device with a diameter of 30 cm and a height of 40 cm at the mouth and the bottom. When planting the mother plant, the covering soil is lower than the container cup of the original seedlings, and it is placed according to the plant spacing × row spacing of 40 cm × 50 cm to cultivate the ear picking mother plant. For mother plants with a ground diameter of 0.5 cm, cut off the trunk at 30 cm from the base. Apply 5 g of refined organic fertilizer + 10 g of compound fertilizer (N:P:K=15:15:15) produced by Beijing Huiminda Science and Technology Development Center to each mother plant according to the conventional method, water thoroughly, and follow the conventional method. Methods Spraying 6-furfurylaminopurine (6KT) with a mass concentration of 30 mg.L -1 . In January, the secondary side branches that germinated on the main trunk of the mother plant without new shoots were collected, and the side branches were cut into cuttings with a length of 10 cm according to the conventional method. After the cuttings are treated with the rooting accelerator, the cuttings are placed in a container equipped with cutting substrates in the awning according to the conventional method. The components and parts by weight of the cutting substrates are: 70 parts of yellow heart soil, 29 parts of coconut peat, and 1 part of vermiculite. Cover the cutting bed with a plastic film, lift the film every 6 days, water and spray the mixed nutrient solution composed of 0.2% polypeptide urea+0.3% ferrous sulfate according to the dry and wet conditions of the substrate according to the conventional method. After the cuttings take root and stabilize, remove the film. After collecting the cuttings, each mother plant was topdressed with 10 g of refined organic fertilizer + 5 g of compound fertilizer (N:P:K=15:15:15) produced by Beijing Huiminda Science and Technology Development Center. On average, 10 cuttings were obtained from each mother plant, and the cutting survival rate was 91.7%.
实施例Example 22 ：:
将优良无性系苗木种植于装有母株基质的母株培育器内，母株基质其组分和重量份数为：森林表土10份、木糠10份、经过熟化的农家肥0.3份，母株培育器是选用口和底的直径为30 cm、高为50 cm的控根育苗器。种植母株时覆土低于原苗木的容器杯口，并按株距×行距为40 cm×60 cm摆放，培育采穗母株。对地径达到0.6 cm的母株，于基部30 cm处截断主干。按常规方法对每株母株追施由北京惠民达科技发展中心生产的精制有机肥10 g + 复合肥(N:P:K=15:15:15) 8 g，浇透水，并按常规方法喷施质量浓度为50 mg.L-1的6-糠氨基嘌呤(6KT)。2月采集母株主干上萌生未抽新梢的的二级侧枝，把侧枝按常规方法剪成长度为12 cm的插穗。用生根促进剂处理插穗后，按常规方法将插穗扦插到荫棚内装有扦插基质的容器里，扦插基质其组分和重量份数为：黄心土70份、椰糠29份、蛭石1份。用塑料薄膜覆盖扦插床，每隔7天掀开薄膜，视基质干湿情况按常规方法浇水和喷施由含有质量浓度为0.3 %多肽尿素 + 0.3 %硫酸亚铁组成的混合营养液，待插穗生根稳定后，除掉薄膜。采集完插穗后，每株母株则追施由北京惠民达科技发展中心生产的精制有机肥10 g+复合肥(N:P:K=15:15:15) 8 g。平均每株母株得插穗11条，扦插成活率95.1 %。 Good clone seedlings are planted in the mother plant incubator that mother plant substrate is housed, and its components and parts by weight of mother plant substrate are: 10 parts of forest topsoil, 10 parts of wood chaff, 0.3 part of mature farmyard manure, mother plant substrate The plant cultivation device is a root-controlling seedling cultivation device with a diameter of 30 cm and a height of 50 cm at the mouth and the bottom. When planting the mother plant, the covering soil is lower than the container cup of the original seedlings, and it is placed according to the plant spacing × row spacing of 40 cm × 60 cm to cultivate the ear picking mother plant. For mother plants with a ground diameter of 0.6 cm, cut off the trunk at 30 cm from the base. Apply 10 g of refined organic fertilizer + 8 g of compound fertilizer (N:P:K=15:15:15) produced by Beijing Huiminda Science and Technology Development Center to each mother plant according to the conventional method, water thoroughly, and follow the conventional method. Methods Spraying 6-furfurylaminopurine (6KT) with a mass concentration of 50 mg.L -1 . In February, the secondary side branches that germinated on the main trunk of the mother plant without new shoots were collected, and the side branches were cut into cuttings with a length of 12 cm according to the conventional method. After the cuttings are treated with the rooting accelerator, the cuttings are placed in a container equipped with cutting substrates in the awning according to the conventional method. The components and parts by weight of the cutting substrates are: 70 parts of yellow heart soil, 29 parts of coconut peat, and 1 part of vermiculite. Cover the cutting bed with a plastic film, lift the film every 7 days, water and spray the mixed nutrient solution composed of 0.3% polypeptide urea+0.3% ferrous sulfate according to the dry and wet conditions of the substrate according to the conventional method, and wait until After the cuttings take root and stabilize, remove the film. After collecting the cuttings, each mother plant was topdressed with 10 g of refined organic fertilizer + 8 g of compound fertilizer (N:P:K=15:15:15) produced by Beijing Huiminda Science and Technology Development Center. On average, 11 cuttings were obtained from each mother plant, and the cutting survival rate was 95.1%.
实施例Example 33 ：:
将优良无性系苗木种植于装有母株基质的母株培育器内，母株基质其组分和重量份数为：森林表土10份、椰糠10份、经过熟化的农家肥0.1份，母株培育器是选用口和底的直径为25 cm、高为50 cm的控根育苗器。种植母株时覆土低于原苗木的容器杯口，并按株距×行距为30 cm×50 cm摆放，培育采穗母株。对地径达到0.8 cm的母株，于基部30 cm处截断主干。按常规方法对每株母株追施由北京惠民达科技发展中心生产的精制有机肥10 g + 复合肥(N:P:K=15:15:15) 5 g，浇透水，并按常规方法喷施质量浓度为20 mg.L-1的6-糠氨基嘌呤(6KT)。1月采集母株主干上萌生未抽新梢的的二级侧枝，把侧枝按常规方法剪成长度为11 cm的插穗。用生根促进剂处理插穗后，按常规方法将插穗扦插到荫棚内装有扦插基质的容器里，扦插基质其组分和重量份数为：黄心土70份、椰糠29份、蛭石1份。用塑料薄膜覆盖扦插床，每隔5天掀开薄膜，视基质干湿情况按常规方法浇水和喷施由含有质量浓度为0.3 %多肽尿素 + 0.2 %硫酸亚铁组成的混合营养液，待插穗生根稳定后，除掉薄膜。采集完插穗后，每株母株则追施由北京惠民达科技发展中心生产的精制有机肥5 g + 复合肥(N:P:K=15:15:15) 5 g。平均每株母株得插穗9条，扦插成活率93.8 %。 Good clone seedlings are planted in the mother plant incubator that mother plant substrate is housed, and its components and parts by weight of mother plant substrate are: 10 parts of forest topsoil, 10 parts of coir peat, 0.1 part of mature farmyard manure, mother plant substrate The plant cultivation device is a root-controlling seedling cultivation device with a diameter of 25 cm and a height of 50 cm at the mouth and the bottom. When planting the mother plant, the covering soil is lower than the container cup of the original seedlings, and placed according to the distance between the plants and the rows of 30 cm × 50 cm, and the mother plant for ear picking is cultivated. For mother plants with a ground diameter of 0.8 cm, cut off the trunk at 30 cm from the base. Apply 10 g of refined organic fertilizer + 5 g of compound fertilizer (N:P:K=15:15:15) produced by Beijing Huiminda Science and Technology Development Center to each mother plant according to the conventional method, water thoroughly, and follow the conventional method. Methods Spraying 6-furfurylaminopurine (6KT) with a mass concentration of 20 mg.L -1 . In January, the secondary side branches that germinated on the main trunk of the mother plant without new shoots were collected, and the side branches were cut into cuttings with a length of 11 cm according to the conventional method. After the cuttings are treated with the rooting accelerator, the cuttings are placed in a container equipped with cutting substrates in the awning according to the conventional method. The components and parts by weight of the cutting substrates are: 70 parts of yellow heart soil, 29 parts of coconut peat, and 1 part of vermiculite. Cover the cutting bed with a plastic film, lift the film every 5 days, water and spray the mixed nutrient solution composed of 0.3% polypeptide urea+0.2% ferrous sulfate according to the dry and wet conditions of the substrate according to the conventional method. After the cuttings take root and stabilize, remove the film. After collecting the cuttings, each mother plant was topdressed with 5 g of refined organic fertilizer + 5 g of compound fertilizer (N:P:K=15:15:15) produced by Beijing Huiminda Science and Technology Development Center. On average, 9 cuttings were obtained from each mother plant, and the survival rate of cuttings was 93.8%.
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