CN109169289A - A kind of crape myrtle tissue culture proliferation seedling rooting medicament and method - Google Patents
A kind of crape myrtle tissue culture proliferation seedling rooting medicament and method Download PDFInfo
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- CN109169289A CN109169289A CN201811310513.7A CN201811310513A CN109169289A CN 109169289 A CN109169289 A CN 109169289A CN 201811310513 A CN201811310513 A CN 201811310513A CN 109169289 A CN109169289 A CN 109169289A
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- medicament
- seedling
- proliferated
- crape myrtle
- tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of crape myrtle tissue culture proliferation seedling rooting medicament and methods, belong to seedling propagation technical field.Medicament effective component of the present invention is indolebutyric acid sylvite, the use of concentration is 300~600mg/L.The present invention also provides a kind of rooting rates, and high, the more crape myrtle tissue culture of number of taking root is proliferated seedling rooting method, i.e., will be proliferated the quickly dipped medicament of seedling base portion, then sunning≤1min is seeded in root media；In the quickly dipped processing, it is proliferated seedling height of seedling >=4cm, the depth that base portion is dipped into medicament is 0.5~1cm, and the quickly dipped time is 5~10s.Crape myrtle tissue culture of the present invention is proliferated seedling rooting rate up to 98% or more, radical 7~9,3.0~6.0cm of root long, can large-area applications in crape myrtle tissue culture be proliferated seedling root induction.
Description
Technical field
The invention belongs to seedling propagation technical fields, and in particular to a kind of crape myrtle tissue culture is proliferated medicament and the side of seedling rooting
Method, the tissue-cultured seedling rooting rate obtained using this method is high, well developed root system, and transplanting survival rate is high.
Background technique
Crape myrtle is defoliation small arbor or the shrub of Lythraceae Lagerstroemia, and pattern is gorgeous, and midsummer blooms, and the florescence is again long, tool
There is the advantages that contamination resistance is strong, adapts to urban environment, it is very popular, it is widely used in afforestation.
Cuttage and grafting method is mostly used to breed nursery stock in common vetch production, seedling raise period is limited, and breeding coefficient is small, and infectivity germ, product
Kind is degenerated fast.Group culturation rapid propagating technology have reproduction speed it is fast, not by season and territory restriction the advantages of, it is a kind of to be worthy to be popularized
Mating system.Tissue-culturing rapid propagation include it is primary, be proliferated, three steps of taking root, wherein root induction step is mostly important, obtains a large amount of
It takes root and the tissue-cultured seedling of well developed root system, is the key that realize scale tissue culture, tissue-cultured seedling transplanting survival rate can be significantly improved,
Production cost is reduced, the economic benefit of tissue-cultured seedling is improved.
By literature search, crape myrtle tissue culture research report is less, uses tissue culture technology also for conventional method, i.e., by explant
Body is seeded in initial culture base, is selected aseptic seedling and is inoculated into proliferated culture medium, and seedling to be proliferated grows to certain altitude, is inoculated into
Root induction in root media.Such as a kind of publication number CN 04126506A " method for tissue culture of American Red rocket crape myrtle "
And a kind of two patents of publication number CN 04604735A " tissue culture and rapid propagation method of U.S.'s red autumnal leaves crape myrtle ", it is to select annual shoot
Item does explant, after sterilization, is seeded in initial culture base and induces Multiple Buds, carries out shoot proliferation using Multiple Buds
Culture, then be transferred on root media and carry out rooting induction, eventually form rooted seedling.It there is no at present a kind of using proliferation seedling speed
Dip in the method that medicament carrys out root induction.
Summary of the invention
The present invention overcomes the shortcomings that prior art and insufficient, provides a kind of crape myrtle tissue culture proliferation seedling root induction medicament and side
Method improves crape myrtle and is proliferated seedling rooting rate, promotes crape myrtle tissue-cultured seedling well developed root system.
In order to achieve the above object, technical solution provided by the invention are as follows:
The 1000ml is taken root containing 300~600mg of indolebutyric acid sylvite in medicament, remaining is water.
Preferably, 400~500mg of indolebutyric acid sylvite is contained in the 1000ml medicament, remaining is water.
The preparation method of the medicament uses this field conventional means: after indolebutyric acid sylvite is dissolved with a small amount of water, then
Add water constant volume, stir evenly and be stored in brown glass container, is kept in dark place under 5 ± 2 DEG C of temperature environments spare.In use,
According to requirement, be dispensed into the sterilizing of glass container mesohigh, with sterilizing with.
The present invention also provides a kind of methods of crape myrtle tissue culture proliferation seedling root induction, include the following steps: that seedling will be proliferated
The quickly dipped above-mentioned medicament of base portion, quickly dipped rear sunning≤1min, is then seeded in root media；Seedling is proliferated in the quickly dipped processing
The depth that base portion is dipped into medicament is 0.5~1cm, and the quickly dipped time is 5~10s.
Below with reference to principle, the invention will be further described:
Conventional tissue-culturing rapid propagation nursery, root induction mainly in basic medium be added auxin (such as heteroauxin,
Indolebutyric acid, methyl α-naphthyl acetate etc.), tissue-cultured seedling absorbs slowly, and root restriction and adventitious root can just be induced by taking more time, and also be slowed down
Root growth speed.The present invention is using the quickly dipped proliferation seedling base portion of indolebutyric acid sylvite, because indolebutyric acid sylvite is to use water
Dissolution will not damage proliferation seedling base cells and tissue, and indolebutyric acid sylvite is also absorbed and used quickly, and it is indefinite to accelerate
The induced velocity of root promotes the growth and development of root system.The present invention take root medicament concentration it is most important, concentration is too low, induces
Adventitious root out is few, and excessive concentration then induces callus, influences induction and the root growth of adventitious root.
Using the quickly dipped chemicals treatment of the present invention and rooting method, 4~6 days after inoculation start to take root, raw after 28~30 days
Root rate is up to 98% or more, the sturdy prosperity of the root system induced, radical 7~9,3.0~6.0cm of root long, can large-area applications in
Crape myrtle tissue culture is proliferated seedling root induction.
Specific embodiment
Embodiment 1
Medicament each component content is (preparing 1000ml medicament stock dosage):
Indolebutyric acid sylvite 350mg, water are settled to 1000ml.
The integrated embodiment that system's the present embodiment set forth below is applied in 3 kinds of crape myrtle tissue culture proliferation seedling root inductions
Card.
Medicament of taking root is prepared using the above method first, indolebutyric acid potassium salts content is 500mg, high pressure in 1000ml medicament
It is after disinfection to reserve.Clip crape myrtle tissue culture is proliferated seedling, it is desirable that height of seedling >=4cm removes lower blade, retains 2~4, top leaf, base
Portion is neatly arranged in sterile disk.With tweezers clamp proliferation seedling, by its base portion in medicament of taking root quickly dipped 10s, be dipped into medicament
Depth be 0.5cm, it is quickly dipped after dry 30s, be seeded in the culture medium of 1/2MS+PVP250mg/L+ sucrose 30g/L+ agar 7g/L
Upper culture, preceding 4d keep dark, and the later period is changed to intensity of illumination 2000lux, lighting delay number 12h/d.It investigates within 30 days after inoculation, as a result
As follows (table 1):
Table 1
Embodiment 2
Chemical content are as follows: indolebutyric acid sylvite 400mg, water are settled to 1000ml.Compounding method for medicament and use step are same
Embodiment 1.The quickly dipped 5s of seedling base portion is proliferated to 3 kinds of crape myrtle tissue cultures with above-mentioned medicament, the depth for being dipped into medicament is 1.0cm, after quickly dipped
40s is dried, is seeded on the culture medium of 1/2MS+PVP300mg/L+ sucrose 30g/L+ agar 7g/L and cultivates, preceding 5d keeps dark,
Later period is changed to intensity of illumination 1500lux, lighting delay number 12h/d.It investigates within 30 days after inoculation, rooting rate is respectively purple rhythm crape myrtle
100%, purpurine spirit crape myrtle 100%, Hunan rhythm crape myrtle 100%, number of taking root are respectively 7.6,8.2,7.8.
Claims (6)
1. a kind of crape myrtle tissue culture is proliferated seedling rooting medicament, which is characterized in that contain indolebutyric acid sylvite in the 1000ml medicament
300~600mg, remaining is water.
2. medicament as described in claim 1, which is characterized in that in the 1000ml medicament containing indolebutyric acid sylvite 400~
500mg。
3. a kind of crape myrtle tissue culture is proliferated seedling rooting method, which is characterized in that the method is will to be proliferated the quickly dipped right of seedling base portion to want
Medicament described in asking 1, quickly dipped rear sunning≤1min, is then seeded in the 1/2MS+PVP250~300mg/L+ sucrose sterilized
In the culture medium of 6~8g/L of 30g/L+ agar, 28~30d of culture in culturing room is moved on to.
4. method as claimed in claim 3, which is characterized in that the disinfection refers in 1.1kg/cm2High pressure under, when disinfection
Between be 15~20min.
5. method as claimed in claim 3, which is characterized in that the proliferation height of seedling degree >=4cm, base portion are dipped into the depth of medicament
For 0.5~1cm, the quickly dipped time is 5~10s.
6. method as claimed in claim 3, which is characterized in that 24 ± 2 DEG C of room temperature of culture, culture 3~5d early period are dark
Culture, it is 1500~2000lux, lighting delay number 12h/d that the later period, which is changed to intensity of illumination,.
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| CN201811310513.7A CN109169289A (en) | 2018-11-06 | 2018-11-06 | A kind of crape myrtle tissue culture proliferation seedling rooting medicament and method |
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| CN201811310513.7A CN109169289A (en) | 2018-11-06 | 2018-11-06 | A kind of crape myrtle tissue culture proliferation seedling rooting medicament and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113317201A (en) * | 2021-06-25 | 2021-08-31 | 中国林业科学研究院林业研究所 | Method for improving rooting rate of taxus media tissue culture seedlings |
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| CN113317201A (en) * | 2021-06-25 | 2021-08-31 | 中国林业科学研究院林业研究所 | Method for improving rooting rate of taxus media tissue culture seedlings |
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Application publication date: 20190111 |