CN102696488B - Method for rapid propagation of lycoris plant seedlings through hydroponics - Google Patents
Method for rapid propagation of lycoris plant seedlings through hydroponics Download PDFInfo
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- CN102696488B CN102696488B CN 201210235203 CN201210235203A CN102696488B CN 102696488 B CN102696488 B CN 102696488B CN 201210235203 CN201210235203 CN 201210235203 CN 201210235203 A CN201210235203 A CN 201210235203A CN 102696488 B CN102696488 B CN 102696488B
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Abstract
The invention discloses a method for rapid propagation of lycoris plant seedlings through hydroponics and aims at providing a lycoris plant asexual rapid propagation method with high reproductive rate for industrialization production of the lycoris plant seedlings. The technical scheme comprises mechanical cutting and induction of adventitious shoots through water pipelines. The method includes following steps: (1) cutting seedballs on a special lycoris seedball cutting machine according to a shape of a Chinese character mi; (2) soaking mother balls in culture solution for 30 minutes, fishing out and drying in shade; (3) inducing adventitious shoots through the water pipelines; and (4) transplanting in a greenhouse. The method for rapid propagation of lycoris plant seedlings through the hydroponics can greatly improve reproductive rate of lycoris aurea, reproduction ratio of one mother ball is 14 times of that of a natural son ball, and the method is suitable for industrialization large-scale production of the lycoris plant seedlings.
Description
Technical field
The invention belongs to the agricultural cience and farming techniques field, a kind of propagation method of plant seedling, specifically a kind of method of breeding the lycoris plants seedling with water culture fast.
Background technology:
Amaryllidaceae (Amarylllidaceae) Lycoris is China and some national endemic genuses of East Asia, and its feature is relatively stable, is herbaceos perennial.Amrallid contains with it multiplely has the alkaloid component of biologically active and pharmacologically active to cause countries in the world pharmaceuticals researcher's concern.Lycoris plants is used as medicinal very early, and according to the Compendium of Material Medica record, lycoris plants has detoxifcation, timid phlegm, diuresis, emetic function.The chemical composition of lycoris plants mainly comprises alkaloid and chromocor compound aspect.
The propagation method of lycoris plants is commonly seminal propagation and the breeding of natural bulb separation at present, can not meet the need of market, the inventor of our company once studied golden flower short-tube lycoris asexual rapid propagation method, make the above golden flower short-tube lycoris of growth in 3 years can breed 12-15 kind ball every year, it is 6-10 times that the nature bulb separation is bred, the inventive method obtains national patent power through declaring, denomination of invention " asexual rapid propagation method of golden lycoris " patent No. ZJ200510003190.3, the inventor is further finding in the research, " asexual rapid propagation method of golden lycoris " (abbreviation background method) also comes with some shortcomings, comprise the vegetative propagation of (1) background technology at a kind of short-tube lycoris Lycoris aurea in the lycoris plants (being the golden flower short-tube lycoris), do not mention other lycoris plants effective yet, (2) culture fluid becomes waste liquid after soaking the mother bulb of cutting open in the background technology, effectively do not utilize, the contaminated environment of abandoning, processing increased processing cost, (3) operation in the background technology is manual operations entirely, speed is slow, benefit is low, can't suitability for industrialized production, (4) bringing out indefinite bud in the background technology is to carry out in matrix soil, and cost is higher.
Summary of the invention:
Purpose of the present invention, exactly at the problem that exists in the above-mentioned background technology, work out a kind of method of breeding the lycoris plants seedling with water culture fast, the present invention is different with background technology with the method for bringing out indefinite bud to the formulation ratio of culture fluid, it is change to background technology, be easy to mechanized operation than background technology, be suitable for batch production production, and do not cause environmental pollution.
The present invention is a kind of to breed the method for lycoris plants seedling fast with water culture, and comprise following concrete steps: (1) is down cut the lycoris plants bulb open by the rice font from the basal disc top, and depth of cut is 1/2～2/3 of a mother bulb height; (2) the above-mentioned mother bulb of cutting open was soaked 30 minutes with culture fluid, pull out and dry in the shade; (3) mother bulb that will dry in the shade brings out indefinite bud; (4) indefinite bud that will open up leaf disperses to transplant to booth, it is characterized in that step (1) Lycoris stem is classified before cutting, and selecting diameter is bulb work kind of the ball of 5～5.5cm, will plant ball and cut with cutter special; Step (2) culture fluid is to be diluted to 1000 times solution by the mixed solution that following composition forms through water, and the volume ratio of each composition is a thimerosal in the described mixed solution: 36～38%, medium: 3～4%, growth regulator: 58～61%; Described thimerosal is made up of concentration 80% carbendazim 20g/L, tmtd 20g/L, 84 thimerosal 10ml/ L; Medium is to be the mixed solution that the 6BA of 0.1mg/L, IAA that concentration is 0.15mg/L form by B5 medium, concentration, and the volume ratio of each composition is a B5 medium in this mixed solution: 13～15%, 6BA:72～74%, IAA:13～15%; Described growth regulator is to be that 1.5% the gibberellin crystal grain aqueous solution, concentration are the mixed solution that 1.5～2.0% potassium hydroxide aqueous solution forms by concentration, and the volume ratio of each composition is the gibberellin crystal grain aqueous solution in this mixed solution: 70～75%, potassium hydroxide aqueous solution: 25～30%; The mother bulb that step (3) will be dried in the shade is closely planted on the water planting pipeline, with 5000 times of the above-mentioned culture fluid waste liquid dilute with waters that soaked mother bulb, indoor have natural scattering light, and the air humidity control is 70%～90%, temperature is controlled between 22 ℃～28 ℃, and indefinite bud was brought out in the circulation maintenance in 3 months; The indefinite bud that step (4) will be opened up leaf disperses to transplant to the booth compost, and shade density is 50% in the booth, and the air humidity control is 60%～90%, and temperature is controlled between 15 ℃～30 ℃, and soil humidity is between 60%～70%, and maintenance is carried out seedling and gathered after 5 months.
The equipment that step (3) is brought out indefinite bud is special-purpose water planting pipeline; Pipe diameter is the PVC semicircle pipe of 150mm, and solution deep is no more than 30mm, and flow velocity is 0.8-1.0 meters/minutes; Distance 1～2mm between the short-tube lycoris bulb, the short-tube lycoris head inserts solution deep 25mm, and the solution that brings out indefinite bud circulation maintenance is to be got by 5000 times of the culture fluid waste liquid dilutions of soaking mother bulb, circulates with pump.
In step (2), the volume ratio of each composition is thimerosal 36%, medium 3.5%, growth regulator 60.5% in the described mixed solution, and described thimerosal is made up of concentration 80% carbendazim 20g/L, tmtd 20g/L, 84 thimerosal 10ml/ L; Medium is to be the mixed solution that the 6BA of 0.1mg/L, IAA that concentration is 0.15mg/L form by B5 medium, concentration, and the volume ratio of each composition is B5 medium 14%, 6BA:73%, IAA:13% in this mixed solution; Described growth regulator is to be that 1.5% the gibberellin crystal grain aqueous solution, concentration are the mixed solution that 1.6% potassium hydroxide aqueous solution forms by concentration, and the volume ratio of each composition is the gibberellin crystal grain aqueous solution 75%, potassium hydroxide aqueous solution 25% in the mixed solution.
The mother bulb that step (3) will be dried in the shade is closely planted on the water planting pipeline, and with 5000 times of above-mentioned culture fluid waste liquid dilutions of soaking mother bulb, with the pump circulation, indoor have natural scattering light, and the air humidity control is 80%, 25 ℃ of temperature controls, and indefinite bud was brought out in the circulation maintenance in 3 months.
The indefinite bud that step (4) will be opened up leaf disperses to transplant to the booth compost, and shade density is 50% in the booth, and the air humidity control is 80%, and temperature is controlled between 25 ℃, and soil humidity is 70%, and maintenance is carried out seedling and gathered after 5 months.
In the thimerosal of the present invention, tmtd, English general Thiram by name, other titles have thiuram, TMTD, A Ruisheng.Molecular formula C
6H
12N
2S
4, structural formula
84 thimerosals are that a kind of hypochlorous sodium is main high effect disinfectants, and main component is clorox NaClO, and 84 thimerosals are the disinfectants that are mixed of clorox and surfactant.
In the medium of the present invention, B5 medium is a kind of medium of design such as Gan Boge Gamborg.
IAA,Chinese: indole-3-acetic acid, other titles: heteroauxin or 3-indolyl acetic acid, the plant hormone of a kind of energy coordinate plant growth and growth.
Used other thimerosals of the present invention are identical with background technology with composition in the medium.
The present invention and " asexual rapid propagation method of golden lycoris " patent comparative illustration
In " asexual rapid propagation method of golden lycoris " patent No. ZJ200510003190.3 patent only at the Lycoris aurea in the lycoris plants, and this patent is through testing: because of the compound method difference of culture fluid, and matrix of no use is brought out indefinite bud and is adopted and cultivate 5000 times of waste liquid dilutions and follow and cultivate also that to bring out indefinite bud all effective to the quick breeding of different lycoris plants;
2. " asexual rapid propagation method of golden lycoris " patent No. ZJ200510003190.3 patent is to operate by hand, speed is slow, benefit is low, can't batch production production, and this patent utilizes homemade special equipment, adopts mechanized operation, and speed is fast, high efficiency is suitable for the seedling that lycoris plants is produced in batch production;
3. the described cultivation waste liquid of " asexual rapid propagation method of golden lycoris " patent No. ZJ200510003190.3 patent does not utilize and discards after handling, increased processing cost, deal with improperly also and can cause environmental pollution, and this specially will be cultivated 5000 times of waste liquid dilutions and follow also cultivation and bring out indefinite bud, reduce treatment cost of waste liquor, can not cause environmental pollution;
4. " asexual rapid propagation method of golden lycoris " patent No. ZJ200510003190.3 patent is described is to hand over after culture fluid soaks airing to be inverted in the matrix, indefinite bud is brought out in cultivation, and being the mother bulb that dries in the shade, this patent closely plants on the water planting pipeline, with 5000 times of above-mentioned cultivation waste liquid dilutions, indefinite bud was brought out in the circulation maintenance in 3 months, matrix of no use, adopt the pipeline water planting, the aqueous solution is recycling, has saved cost, has improved benefit;
5, the present invention has prepared the prescription different with background technology and the culture fluid of proportioning according to the water planting characteristics.
Embodiment:
The preparation of embodiment 1, culture fluid:
(1) 360 liters of thimerosals of preparation: in 360 liters of thimerosals, contain the carbendazim 7200g of concentration 80%, tmtd 7200g, 84 thimerosal 3600ml, weighing is water-soluble in proportion.
(2) 35 liters of culture medium solutions of preparation: get 25.6 liters of 6BA, 4.5 liters of the 0.15mg/L IAA of 4.9 liters of B5 mediums, concentration 0.1mg/l, mix.
(3)) 605 liters of growth regulators of preparation: 453.8 liters of 1.5% Gibberellins solution, 151.2 liters of 1.6% potassium hydroxide solutions, both are mixed, 605 liters of growth regulators.
At last 360 liters of thimerosals, 35 liters of culture medium solutions, 605 liters of growth regulators are mixed 1000 liters of culture fluids, get 1000 times of above-mentioned culture fluid dilute with waters, be used to soak mother bulb.
Embodiment 2: selecting diameter is 10000 on 5.0-5.5 short-tube lycoris bulbs, will down cut open by the rice font from the basal disc top on the lycoris plants bulb kind ball cutter special, and depth of cut is 1/2～2/3 of a mother bulb height; The mother bulb of cutting open soaked 30 minutes with culture fluid, pulled out and dried in the shade; The mother bulb that will dry in the shade is closely planted on the water planting pipeline, dilutes 5000 times with the above-mentioned culture fluid waste liquid that soaked mother bulb, follows maintenance and brings out indefinite bud in 3 months; The indefinite bud of opening up leaf is disperseed to transplant to the booth compost, and maintenance is carried out seedling and is gathered after 5 months.Get 145000 strains of lycoris plants seedling, the propagation ratio is 1:14.
Embodiment 3: selecting diameter is 10000 on 5.0-5.5 short-tube lycoris bulbs, will down cut open by the rice font from the basal disc top on the lycoris plants bulb kind ball cutter special, and depth of cut is 1/2～2/3 of a mother bulb height; The mother bulb of cutting open soaked 30 minutes with culture fluid, pulled out and dried in the shade; The mother bulb that will dry in the shade is closely planted on the water planting pipeline, dilutes 5000 times with the above-mentioned culture fluid waste liquid that soaked mother bulb, follows maintenance and brings out indefinite bud in 3 months; The indefinite bud of opening up leaf is disperseed to transplant to the booth compost, and maintenance is carried out seedling and is gathered after 5 months.Get 145000 strains of lycoris plants seedling, the propagation ratio is 1:14.5.
Claims (5)
1. breed the method for lycoris plants seedling fast with water culture for one kind, comprise following concrete steps: (1) is down cut the lycoris plants bulb open by the rice font from the basal disc top, and depth of cut is 1/2～2/3 of a mother bulb height; (2) the above-mentioned mother bulb of cutting open was soaked 30 minutes with culture fluid, pull out and dry in the shade; (3) mother bulb that will dry in the shade brings out indefinite bud; (4) indefinite bud that will open up leaf disperses to transplant to booth, it is characterized in that step (1) Lycoris stem is classified before cutting, and selecting diameter is bulb work kind of the ball of 5～5.5cm, will plant ball and cut with cutter special; Step (2) culture fluid is to be diluted to 1000 times solution by the mixed solution that following composition forms through water, and the volume ratio of each composition is a thimerosal in the described mixed solution: 36～38%, medium: 3～4%, growth regulator: 58～61%; Described thimerosal is made up of concentration 80% carbendazim 20g/L, tmtd 20g/L, 84 thimerosal 10ml/ L; Medium is to be the mixed solution that the 6-BA of 0.1mg/L, IAA that concentration is 0.15mg/L form by B5 medium, concentration, and the volume ratio of each composition is a B5 medium in this mixed solution: 13～15%, concentration is that 0.1mg/L 6-BA:72～74%, concentration are 0.15mg/L IAA:13～15%; Described growth regulator is to be that 1.5% the crystal grain gibberellin aqueous solution, concentration are the mixed solution that 1.5～2.0% potassium hydroxide aqueous solution forms by concentration, and the volume ratio of each composition is that concentration is the 1.5% crystal grain gibberellin aqueous solution in this mixed solution: 70～75%, concentration is 1.5～2.0% potassium hydroxide aqueous solutions: 25～30%; The mother bulb that step (3) will be dried in the shade is closely planted on the water planting pipeline, with 5000 times of the above-mentioned culture fluid waste liquid dilute with waters that soaked mother bulb, indoor have natural scattering light, and the air humidity control is 70%～90%, temperature is controlled between 22 ℃～28 ℃, and indefinite bud was brought out in the circulation maintenance in 3 months; The indefinite bud that step (4) will be opened up leaf disperses to transplant to the booth compost, shade density is 50% in the booth, and the air humidity control is 60%～90%, and temperature is controlled between 15 ℃～30 ℃, soil humidity is between 60%～70%, and maintenance is carried out seedling and gathered after 5 months.
2. according to claim 1ly a kind ofly breed the method for lycoris plants seedling fast, it is characterized in that it is special-purpose water planting pipeline that step (3) is brought out the equipment of indefinite bud with water culture; Pipe diameter is the PVC semicircle pipe of 150mm, and solution deep is no more than 30mm, and flow velocity is 0.8-1.0 meters/minutes; Distance 1～2mm between the short-tube lycoris bulb, the short-tube lycoris head inserts solution deep 25mm, and the solution that brings out indefinite bud circulation maintenance is to be got by 5000 times of the culture fluid waste liquid dilutions of soaking mother bulb, circulates with pump.
3. a kind of method of breeding the lycoris plants seedling with water culture fast according to claim 1, it is characterized in that in the step (2), the volume ratio of each composition is thimerosal 36%, medium 3.5%, growth regulator 60.5% in the described mixed solution, and described thimerosal is made up of concentration 80% carbendazim 20g/L, tmtd 20g/L, 84 thimerosal 10ml/ L; Medium is to be the mixed solution that the 6-BA of 0.1mg/L, IAA that concentration is 0.15mg/L form by B5 medium, concentration, and the volume ratio of each composition is that B5 medium 14%, concentration are that 0.1mg/L 6-BA:73%, concentration are 0.15mg/L IAA:13% in this mixed solution; Described growth regulator is to be that 1.5% the crystal grain gibberellin aqueous solution, concentration are the mixed solution that 1.6% potassium hydroxide aqueous solution forms by concentration, and the volume ratio of each composition is that concentration is that the 1.5% crystal grain gibberellin aqueous solution 75%, concentration are 1.6% potassium hydroxide aqueous solution 25% in the mixed solution.
4. a kind of method of breeding the lycoris plants seedling with water culture fast according to claim 1, it is characterized in that the mother bulb that step (3) will be dried in the shade closely plants on the water planting pipeline, dilute 5000 times with the above-mentioned culture fluid waste liquid that soaked mother bulb, circulate with pump, indoor have natural scattering light, the air humidity control is 80%, and temperature is controlled 25 ℃, and indefinite bud was brought out in the circulation maintenance in 3 months.
5. a kind of method of breeding the lycoris plants seedling with water culture fast according to claim 1, it is characterized in that step (4) will open up the indefinite bud of leaf and disperse to transplant to the booth compost, shade density is 50% in the booth, the air humidity control is 80%, temperature is controlled between 25 ℃, soil humidity is 70%, and maintenance is carried out seedling and gathered after 5 months.
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| CN111264204A (en) * | 2020-04-16 | 2020-06-12 | 江苏省中国科学院植物研究所 | Method for cutting and rapidly propagating straw lycoris radiata bulbs |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1918960A (en) * | 2006-08-25 | 2007-02-28 | 贵州芊芊园艺新技术发展公司 | Standard planting method of medicinal lycoris radiata |
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| RU1806188C (en) * | 1990-12-21 | 1993-03-30 | Aleksandrova Inessa V | Strain of cultured cells of plant ungernia victoris - a product of alkaloid of galanthamine and lycorine group |
| CN2342582Y (en) * | 1998-02-27 | 1999-10-13 | 黄庆源 | Three-dimensional device for culturing plant in water |
| JP2005198607A (en) * | 2004-01-19 | 2005-07-28 | Tokyo Electric Power Co Inc:The | Method for suppressing weed |
| CN1281114C (en) * | 2004-08-18 | 2006-10-25 | 南京林业大学 | Long tube lycoris fast breeding method |
| CN101366357B (en) * | 2008-09-17 | 2011-02-09 | 杭州植物园 | Method for tissue culture and quick propagation technique of reddish blue spider lily |
| CN102144554B (en) * | 2011-01-25 | 2012-07-04 | 江苏九久环境科技有限公司 | Method for producing lycoris by plant tissue culture |
| CN102217540B (en) * | 2011-05-19 | 2012-07-11 | 南京林业大学 | Quick propagation method for lycoris chinensis |
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| CN1918960A (en) * | 2006-08-25 | 2007-02-28 | 贵州芊芊园艺新技术发展公司 | Standard planting method of medicinal lycoris radiata |
Non-Patent Citations (4)
| Title |
|---|
| 吴云鹏等.忽地笑离体快繁研究.《黑龙江生态工程职业学院学报》.2006,第19卷(第6期),第32-33页. |
| 忽地笑离体快繁研究;吴云鹏等;《黑龙江生态工程职业学院学报》;20061130;第19卷(第6期);第32-33页 * |
| 石蒜属植物的研究进展;赵天荣等;《北方园艺》;20081231;第65-69页 * |
| 赵天荣等.石蒜属植物的研究进展.《北方园艺》.2008,第65-69页. |
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