CN101642050B - Method for enhancing survival rate of grafting sugarcane group seedling - Google Patents
Method for enhancing survival rate of grafting sugarcane group seedling Download PDFInfo
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- CN101642050B CN101642050B CN2009100420115A CN200910042011A CN101642050B CN 101642050 B CN101642050 B CN 101642050B CN 2009100420115 A CN2009100420115 A CN 2009100420115A CN 200910042011 A CN200910042011 A CN 200910042011A CN 101642050 B CN101642050 B CN 101642050B
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Abstract
The invention discloses a method for enhancing survival rate of grafting sugarcane group seedling, which belongs to the technical field of nutritious growth. The soilless culture technique of the continuously flowing film of nutritious liquid is adopted for treating seedlings of sugarcane rooting groups. The method comprises the steps: domesticating; refining; treating the seedlings; grafting thedomesticated seedlings; managing the grafted seedlings daily; planting in farms and adopting nutritious liquids of different concentrations in different time periods in the step of managing the grafted seedlings daily. The method is simple and reliable, is not limited by regional season weather and has low cost. The concentration and the components of the nutritious liquid can be regulated as required; the seedlings of the sugarcane groups have high growth speed and high seedling survival rate; the production period of the seedlings of the sugarcane groups is shortened and the seedlings can be produced in factories and in intensive production.
Description
Technical field
The present invention relates to nutrition culture technique field, more specifically relate to the method that improves survival rate of grafting sugarcane group seedling.
Background technology
Sugarcane is the important sugar [yielding of China, widely plants in China subtropical zone, has irreplaceable important function to promoting producing region economic development and social stability.Because sugarcane is an asexually propagated crop, adopt the stem eye breeding in the production always, sowing quantity is big, and reproduction coefficient is low, badly influences applying of good sugar cane breed, and this just makes that the sugar cane breed promotion rate is slow, and the breeding renewal speed lags behind; After sugar cane breed is planted for many years continuously simultaneously, subject to the disease infringement, wherein infecting repeatedly of pathogeny such as sugarcane ratoon stunting disease, mosaic disease thing causes the yield and quality of sugarcane to descend, and serious danger is coerced the cane planting industry.
The sugarcane tissue culture is a kind of propagation technique efficiently; It also is the effective way that obtains the nontoxic seedling of sugarcane; Sugarcane tissue-culture seedling industry requires high, reproduction speed and fast to the reaction of market demand kind, seedling detoxification high-quality; Seedling production does not receive the influence of area, natural conditions, in the sugarcane industry, is bringing into play important effect.Cultivate virus-elimination seedlings and quicken the breeding breeding through tissue culture technique; Widespread usage in sugarcane industry big countries such as Brazil, the U.S., Australia; The use of tissue cultural seedlings of free makes sugarcane production-increasing up to arriving 20%-40%; Indivedual local even reach 60%, sucrose divides and improves more than 0.5% (absolute value) remarkable in economical benefits.In China, utilize tissue culture to cultivate the cane seedling technology and start late, the tissue cultivating seedling utility ratio is still low, and industrial system is still waiting further perfect.For many years, the research institution in each sugarcane district of China is carrying out the optimization of tissue cultivating seedling industrial technology system always, from the angle of commercialization production; Sterilize at explant; Medium component, a large amount of research has been done in the aspects such as detection of culture environment, tissue cultivating seedling pathogen, has obtained a series of progress; But it is low that tissue cultivating seedling is heeled in survival rate, is " bottleneck " problem that influences the large-scale production and application of sugarcane tissue-culture seedling.The conventional method of heeling in receives environmental influence big, and survival rate is lower, and growth rate is slower, badly influences group and cultivates popularizing of seedling.Because of a little, press for the improvement innovation on the technical method, to improve the survival rate of sugarcane tissue-culture seedling, promote the progress that sugarcane tissue-culture seedling industry not is.
Summary of the invention
The purpose of this invention is to provide a kind of method of heeling in that improves survival rate of grafting sugarcane group seedling, this method can effectively improve the survival rate of heeling in of tissue cultivating seedling, has simple to operate; Cost is low, and the nutrient convenient regulation and control is accurate, characteristics such as fast growth; Be suitable for scale, specialized production.
The object of the invention can be realized through following technical scheme:
A kind of method that improves survival rate of grafting sugarcane group seedling is used for the sugarcane tissue cultivating seedling of taking root is handled, and it is characterized in that comprising the steps:
(1) domestication refining seedling and processing: heeling in the previous day, the sugarcane tissue-culture seedling of having taken root is tamed refining seedling 20～30 hours and removed the yellow leaf of dead leaf, cutting off blade tip, cleaning up with running water.
(2) the domestication seedling is heeled in: the sugarcane tissue-culture seed of step (1) is planted in having the heeling in the groove of mobile nutrient solution, and the control temperature is between 20～35 ℃.
(3) heel in the seedling daily management: after the sugarcane tissue-culture seedling is heeled in, keep the skin wet every day, and according to the conductivity variations situation extra-nutrition liquid of heeling in nutrient solution in the groove; After treating that the sugarcane tissue-culture seedling is heeled in 20 days, the sugarcane health seedling well developed root system, robust plant can carry out the field field planting.
(4) field field planting: the field is arrived in the timely field planting of sugarcane tissue-culture seedling of step (3).
Beneficial effect of the present invention is following:
The present invention filters out the nutrient prescription that is fit to the growth of sugarcane tissue-culture seedling through research, heels in device in conjunction with the continuous-flow nutrient film of design, can reach the purpose of regulation and control sugarcane seedling growth easily through the nutrient of regulation and control nutrient solution; Have the survival rate of grafting sugarcane group seedling height, advantages such as sugarcane seedling fast growth, present device is simple and easy; Cost is lower; Efficient is high, is fit to scale, specialized production, and the development that promotes sugarcane tissue-culture seedling industrial system is significant.
Figure of description
Fig. 1 heels in the groove sketch map for what use among the present invention.
Embodiment
A kind of method that improves survival rate of grafting sugarcane group seedling is used for the sugarcane tissue cultivating seedling of taking root is handled, and it comprises the steps:
1. domestication refining seedling and processing: heeling in the previous day, the sugarcane tissue-culture seedling of having taken root is being placed in the environment of shady and cool ventilation and tames the refining seedling about 24 hours, the yellow leaf of dead leaf is removed in the back, cuts off blade tip, cleans up with running water.
2. the domestication seedling is heeled in: the sugarcane tissue-culture seedling of step 1 is planted in having the heeling in the groove of mobile nutrient solution by seeding row spacing 3 * 5cm kind; The control temperature is between 20～30 ℃, and humidity is between 60～80%, and the sugarcane tissue-culture seedling is heeled in early stage; Should adopt the low concentration nutrient solution; After heel in the work of seedling one-tenth, heeling in the work of seedling one-tenth, the nutrient solution nutrient density strengthens according to upgrowth situation gradually.Generally speaking, heel in the 1st～6 day, adopt 0.25 times of nutrient solution, heel in the 6th～12 day, adopt 0.5 times of nutrient solution, adopt 1 times of nutrient solution after heeling in 12 days.
3. heel in the seedling daily management: the sugarcane tissue-culture seedling is heeled in the process, keep the skin wet every day, and according to per 3 days extra-nutrition liquid of the conductivity variations situation of nutrient solution, regulates pH between 5.6～6.5; Heel in about the 10th day, the sugarcane tissue-culture seedling is extracted a large amount of young leaves out, and it is short strong to carry out leaf-cutting, and leaf-cutting is short weekly strengthens once; After waiting to heel in the 20th day, sugarcane tissue-culture shoot root system is flourishing, and robust plant can carry out the field field planting.
4. field field planting: the sugarcane tissue-culture seedling is extracted from heel in groove; It is long to cut off blade 2/5 left and right sides leaf; After again the water-loss reducer by 1 times of nutrient solution configuration being dipped in sugarcane tissue-culture shoot root portion, the tissue cultivating seedling root is wrapped up (about every bag 100 strains) with plastic film, field planting is to the field in 48 hours.
Wherein, the compound method of 1 times of nutrient solution in the step 4 is:
1) component and concentration (g/1000L):
KNO3 252；
Ca(NO
3)
2.4H
2O 590；
MnCl
2.4H
2O 1.8；
KH
2PO
4 139；
K
2SO
4 44；
Na
2SiO
3.9H
2O 100；
ZnSO
4.7H
2O 0.2；
CuSO
4.5H
2O 0.1；
H
3BO
3 3.0；
MgSO
4.7H
2O 246；
FeSO
4.7H
2O 14；
Na
2-EDTA 19。
2) compound method (1000L): in the nutrition liquid pool, add the water of about 800L earlier, using sulfuric acid or potassium hydroxide solution to regulate pH is between 5.6～6.0, again with 590 gram Ca (NO
3)
2.4H
2Pour in the nutrition liquid pool after the O dissolving, and stir, again other elements are added the nutrition liquid pool respectively, adding water to solution 1000L at last and regulating pH is 5.6～6.0.
0.5 times of nutrient solution compound method in the step 4 is: 1 times of nutrient solution is formulated as 1/2 concentration, i.e. 1 times of nutrient solution: water=1: 1.
0.25 times of nutrient solution compound method in the step 4 is: 1 times of nutrient solution is made as 1/4 concentration, i.e. 1 times of nutrient solution: water=1: 3.
Mobile nutrient solution in the step 2 is 0.25 times of nutrient solution.
The preparation of heeling in groove in the step 2: the structure of heeling in groove is as shown in Figure 1; Heel in groove and comprise pallet 1 and fixed support 2; Fixed support 2 is fixed in the pallet 1 by line-spacing 6cm, and fixed support 1 is advisable fixedly heeling in seedling apart from the tray bottom height, heels in groove and can make sandwich construction; Multilayer is heeled in groove and is supported with feet 3, and upper and lower layer is heeled between the groove 1 and installed communicating pipe 4.
Can place in the greenhouse heeling in groove; Also can place outdoorly, place the outdoor groove top of heeling in to take rain shade, during use heeling in groove; Start nutrient film flow device (not shown); The nutrient solution for preparing is joined in the superiors' pallet of heeling in groove, and the nutrient solution of upper tray can be heeled in concentrated flow through lower floor and return in the nutrient solution storagetank 5 (pallet 1 is adopted in this enforcement) through flowing into one deck pallet down communicating pipe 4 at last; Nutrient solution in the storagetank 5 is withdrawn into the superiors through water pump and heels in groove, forms the nutrient solution continuous-flow system.
In heeling in seedling daily management process, control the flow velocity of nutrient solution, make and heel in that the nutrient solution flow velocity remains a constant speed about 0.05 meter per second in the groove, the nutrient film degree of depth is about 0.3～0.5cm.
Claims (1)
1. method that improves survival rate of grafting sugarcane group seedling is used for the sugarcane tissue cultivating seedling of taking root is handled, and it is characterized in that comprising the steps:
(1) domestication refining seedling and processing: heeling in the previous day, the tissue cultivating seedling of having taken root was tamed the refining seedling 20～30 hours; And remove the yellow leaf of dead leaf, and cut off blade tip, clean up with running water;
(2) the domestication seedling is heeled in: the sugarcane tissue-culture seed of step (1) is planted in having the heeling in the groove of mobile nutrient solution, and the control temperature is between 20～35 ℃;
(3) heel in the seedling daily management: after the sugarcane tissue-culture seedling is heeled in, keep the skin wet every day, and according to the conductivity variations situation extra-nutrition liquid of heeling in nutrient solution in the groove; After heeling in 10 days, the sugarcane tissue-culture seedling is extracted a large amount of young leaves out, and it is short strong to carry out leaf-cutting; After waiting to heel in 20 days, sugarcane tissue-culture shoot root system is flourishing, and robust plant can carry out the field field planting;
(4) field field planting: the field is arrived in the timely field planting of sugarcane tissue-culture seedling of step (3);
Sugarcane tissue-culture seedling in step (3) is heeled in the 1st～6 day, and the nutrient solution of heeling in the groove adopts 0.25 times of nutrient solution, heels in the 7th～12 day, adopts 0.5 times of nutrient solution, heel in 12 days after 1 times of nutrient solution of employing;
Nutrient solution described in the step (2) is 0.25 times of nutrient solution;
Wherein, the compound method of 1 times of nutrient solution is:
1) component and concentration (g/1000L)
KNO
3 220～280；
Ca(NO
3)
2.4H
2O 560～620；
MnCl
2.4H
2O 1.8；
KH
2PO
4 120～160；
K
2SO
4 40～50；
Na
2SiO
3.9H
2O 90～110；
ZnSO
4.7H
2O 0.2；
CuSO4.5H2O 0.1；
H3BO3 3.0；
MgSO
4.7H
2O 230～260；
FeSO
4.7H
2O 12～16；
Na
2-EDTA 18～20；
2) compound method: in the nutrition liquid pool, add the water of about 800L earlier, using sulfuric acid or potassium hydroxide solution to regulate pH is between 5.6～6.0, with Ca (NO
3)
2.4H
2Pour in the nutrition liquid pool after the O dissolving, and stir, other elements are added respectively in the nutrition liquid pool again, adding water to solution 1000L at last and regulating pH is 5.6～6.0;
0.5 doubly the compound method of nutrient solution is: with 1 times of nutrient solution dilution is 1/2 concentration;
0.25 the compound method of cultivation nutrient fluid is: with 1 times of nutrient solution dilution is 1/4 concentration.
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| CN101642050B true CN101642050B (en) | 2012-02-01 |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498912B (en) * | 2011-11-23 | 2014-07-23 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
| CN103392574B (en) * | 2013-08-02 | 2014-09-10 | 中国烟草总公司重庆市公司烟草科学研究所 | Method for growing seedlings by using intermittent flow type nutrient liquid film |
| CN103503775A (en) * | 2013-10-09 | 2014-01-15 | 广州甘蔗糖业研究所 | Sugarcane tissue culture seedling potassium nutrition research method |
| CN104137723B (en) * | 2014-07-23 | 2016-08-24 | 广西南亚热带农业科学研究所 | Sugar-cane tissue culture seedlings auto spraying method for culturing seedlings |
| CN104542262B (en) * | 2015-01-28 | 2017-02-22 | 云南省农业科学院甘蔗研究所 | Method for increasing maturing rate in sugarcane hybrid seed production |
| CN104823848B (en) * | 2015-05-05 | 2017-02-22 | 广州甘蔗糖业研究所 | Root-inducing strong seedling medium for sugarcane and direct field planting method of sugarcane tissue culture bottle seedling |
| CN105875334A (en) * | 2016-05-04 | 2016-08-24 | 罗江县德金种植协会 | Temporary-planting seedling cultivation method of idesia polycarpa |
| CN106332648A (en) * | 2016-08-30 | 2017-01-18 | 广西壮族自治区农业科学院甘蔗研究所 | Single-plant temporary planting method of sugarcane tissue culture rooting bottle seedling |
| CN107047277A (en) * | 2017-05-03 | 2017-08-18 | 蚌埠市宝煦家庭农场 | A kind of method for culturing seedlings for improving sugarcane germination percentage |
| CN108055997A (en) * | 2017-12-05 | 2018-05-22 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of high density temporary planting method of sugar-cane tissue culture seedlings |
| CN108718981A (en) * | 2018-08-14 | 2018-11-02 | 柳州市合联农业有限公司 | A kind of method of sugar-cane tissue culture seedlings field production |
| CN109220748B (en) * | 2018-11-02 | 2023-12-19 | 广西壮族自治区农业科学院 | Sugarcane water planting method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1429473A (en) * | 2001-12-29 | 2003-07-16 | 刘伟 | Seed breeding method of potato detoxified small seed potato |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1429473A (en) * | 2001-12-29 | 2003-07-16 | 刘伟 | Seed breeding method of potato detoxified small seed potato |
Non-Patent Citations (2)
| Title |
|---|
| 何为中等.影响甘蔗组培苗假植成活率的几个因素的研究.《甘蔗糖业》.2002,(第2期),第10-13页. * |
| 陈仲华等.苗圃基质和假值时期对甘蔗组培苗假植成活率的影响.《甘蔗糖业》.2007,(第4期),第13-15、24页. * |
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