CN107047277A - A kind of method for culturing seedlings for improving sugarcane germination percentage - Google Patents
A kind of method for culturing seedlings for improving sugarcane germination percentage Download PDFInfo
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- CN107047277A CN107047277A CN201710305279.8A CN201710305279A CN107047277A CN 107047277 A CN107047277 A CN 107047277A CN 201710305279 A CN201710305279 A CN 201710305279A CN 107047277 A CN107047277 A CN 107047277A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention mainly relates to planting technology field, a kind of method for culturing seedlings for improving sugarcane germination percentage is disclosed, including：It is prepared by sugarcane kind, the field planting of sugarcane kind, the nursery of sugarcane kind, sugarcane seedling hardening；Soilless culture nursery is carried out, method is simple, can carry out batch breeding, environmental beauty, condition is easily controllable, germination percentage reaches 84.3%, and growing way is neat, grows prosperous, strong stress resistance, hence it is evident that save planting cost, income improves 11.4%；Nursery is carried out after nodal bud is individually cut, seedling culture space is saved, economizes on resources, remaining sugarcane can carry out commercial Application, increase income, and nodal bud is placed in into progress low temperature immersion in soak, soak contains Multiple components, plant extracts can antibacterial bacteriostatic, promote wound healing, it is to avoid it is mouldy, the aminomethylbenzoic acid and methyl jasmonate of addition can promote growing for nodal bud, accelerate germination, improve germination percentage, add the application approach of aminomethylbenzoic acid and methyl jasmonate.
Description
Technical field
The invention mainly relates to planting technology field, more particularly to a kind of method for culturing seedlings for improving sugarcane germination percentage.
Background technology
Sugarcane, is a kind of Common Fruits, containing abundant sugar, moisture, in addition, also containing to human metabolism very
Beneficial vitamin, fat, protein, amino acid, mineral element etc., human body is easily digested and assimilated, and often to eat can
Hypoglycemia is prevented, fatigue-relieving, clearing away summerheat, invigorating the spleen diuresis promotes gastrointestinal function, toxin-expelling and face nourishing.
Sugarcane is planted extensively in China, and nursery is planted using sugarcane stem during plantation, but sugarcane stem plants the germination percentage one of nursery
As can only achieve 60% or so, the very big wasting of resources is caused, hence it is evident that improve planting cost, accordingly, it would be desirable to which a kind of improve sugarcane
The method for culturing seedlings of germination percentage.
The content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of nursery side for improving sugarcane germination percentage
Method.
A kind of method for culturing seedlings for improving sugarcane germination percentage, comprises the following steps：
（1）It is prepared by sugarcane kind：Selection nodal bud is full, sugarcane without disease pest, intercepts nodal bud, and nodal bud both sides sugarcane length is 1.5 ~ 2cm,
Seedling culture space is saved, is economized on resources, remaining sugarcane can carry out commercial Application, increases income, nodal bud is placed in 2 ~ 4 DEG C
In soak, it is standing and soak for 4 ~ 5 hours, obtains sugarcane kind；
（2）Sugarcane kind is colonized：Field planting hole is dug on pearl cotton plate, sugarcane kind traverse is in pearl cotton plate, and nodal bud upward, is colonized,
Neat and tidy, is easy to unified management, must be colonized sugarcane kind plate；
（3）Sugarcane kind nursery：Field planting sugarcane kind plate is lain against on seedling culture frame, the vertical range between two plates is 40 ~ 45cm, is made full use of
Space is planted, planting cost is saved, nutrient solution is sprayed, rearing condition is adjusted, 1 ~ 3 day, temperature was 23 ~ 25 DEG C, ambient humidity is
78 ~ 80%, illumination is 80 ~ 100lx, and adaptability of the increase nodal bud to environment, it is to avoid stronger illumination causes nodal bud to be wilted is stimulated
Nodal bud grows and photosynthetic enhancing, 4 ~ 9 days, temperature is 20 ~ 22 DEG C, ambient humidity be 75 ~ 77%, illumination be 160 ~
180lx, strengthens nodal bud resistance, makes nodal bud sturdy, improves the photosynthesis of nodal bud, accelerates growth, meets nodal bud growth to battalion
The consumption formed point, 10 days and after, temperature is 24 ~ 26 DEG C, ambient humidity is that 73 ~ 75%, illumination is that there is provided fill by 200 ~ 240lx
The illumination of foot and nutritional ingredient, improve the speed of growth of nodal bud, obtain sugarcane seedling；
（4）Sugarcane seedling hardening：When sugarcane seedling length is to 4 ~ 5cm, every morning 9:30~10:30, regulation temperature is 28 ~ 30 DEG C, first
It is kept for 8 ~ 10 minutes, extends 4 ~ 5 minutes daily later, afternoon 4:00~5:00, regulation temperature is 14 ~ 16 DEG C, is kept within first day
6 ~ 8 minutes, extend 3 ~ 4 minutes daily later, strengthen the environmental suitability of nodal bud, improve plantation survival rate, hence it is evident that improve peasant household
Income.
The step（1）Soak, pH be 5.7 ~ 5.9, be made up of the raw material of following parts by weight：Xanthium sibiricum 6 ~
8th, Valerian root P.E 6 ~ 8, aminomethylbenzoic acid 2.7 ~ 2.9, methyl jasmonate 1.6 ~ 1.8, a small amount of 70 ~ 80 DEG C are added by aminomethylbenzoic acid
In hot water, stir to being completely dissolved, be cooled to room temperature, add xanthium sibiricum, Valerian root P.E, methyl jasmonate and often
Warm water, stirs, and the concentration for making methyl jasmonate is 5.2 ~ 5.4mmol/L, and plant extracts being capable of antibacterial bacteriostatic, promotion wound
Mouth healing, it is to avoid mouldy, the aminomethylbenzoic acid and methyl jasmonate of addition can promote growing for nodal bud, accelerate germination, carry
Bud rate occurred frequently, adds the application approach of aminomethylbenzoic acid and methyl jasmonate, changes traditional methyl jasmonate and suppresses rudiment
Effect, obtains soak.
Described xanthium sibiricum, is to crush the achene of Siberian cocklebur, and the volume fraction for adding 30 ~ 40 times of amounts of achene of Siberian cocklebur weight is
78 ~ 80% alcoholic solution, in 40 ~ 42 DEG C, 31 ~ 33kHz ultrasounds 15 ~ 20 minutes, filtering, added into filter residue filter residue weight 50 ~
The volume fraction of 60 times of amounts is 47 ~ 49% alcoholic solution, in 43 ~ 45 DEG C, 31 ~ 33kHz ultrasounds 20 ~ 25 minutes, heating boil to
Volume is original 1/10, filtering, merges all filtrates, and drying to water content is 7 ~ 9%, obtains xanthium sibiricum.
Described Valerian root P.E, valerian is crushed, and adds the water of 100 ~ 120 times of amounts of valerian weight, and heating is decocted after boiling
It is original 1/20 to boil to volume, and filtering adds the alcoholic solution that volume fraction is 90 ~ 92% into filtrate, and the end to alcohol is dense
Spend for 45 ~ 47%, 12 ~ 14 hours are stood in 2 ~ 4 DEG C, filtering takes precipitation, and drying to water content is 8 ~ 10%, obtains Valerian root P.E.
Described methyl jasmonate, purity is 98 ~ 100%.
The step（3）Nutrient solution, be made up of the raw material of following parts by weight：Urea 21 ~ 23, calcium superphosphate 13 ~ 15, sulphur
Sour potassium 15 ~ 17, manganese sulfate 4 ~ 6, thiamine 2.5 ~ 2.7, inositol 1.7 ~ 1.9, now with the current, 1 ~ 3 day, the mass concentration of nutrient solution
For 262.3 ~ 263.7mg/L, spray within every 2 hours 1 time, 4 ~ 9 days, mass concentration was 267.2 ~ 268.6mg/L, sprinkling 1 in every 2 hours
It is secondary, 10 days and after, mass concentration be 270.4 ~ 271.8mg/L, every 4 hours spray 1 time.
The step（4）Hardening, continuous hardening 10 ~ 12 days.
It is an advantage of the invention that：A kind of method for culturing seedlings for raising sugarcane germination percentage that the present invention is provided, carries out soilless culture
Nursery, method is simple, can carry out batch breeding, environmental beauty, condition is easily controllable, and germination percentage reaches 84.3%, and growing way is neat, raw
Long prosperous, strong stress resistance, hence it is evident that save planting cost, income improves 11.4%；Nursery is carried out after nodal bud is individually cut, is saved
Seedling culture space is saved, is economized on resources, remaining sugarcane can carry out commercial Application, increases income, nodal bud is placed in soak
Carry out low temperature immersion, soak contain Multiple components, plant extracts can antibacterial bacteriostatic, promote wound healing, it is to avoid it is mouldy, plus
The aminomethylbenzoic acid and methyl jasmonate entered can promote growing for nodal bud, accelerate germination, improve germination percentage, add ammonia first
The application approach of benzoic acid and methyl jasmonate, changes the effect that traditional methyl jasmonate suppresses rudiment；Sugarcane kind is colonized in treasure
On pearl cotton plate, neat and tidy is easy to unified management, and field planting sugarcane kind plate is lain against on seedling culture frame, makes full use of plantation space, section
About planting cost, gradually changes rearing condition, first high temperature, high humidity, low-light spray the nutrient solution of low concentration, increase after field planting
Adaptability of the nodal bud to environment, it is to avoid stronger illumination causes nodal bud to be wilted, stimulates nodal bud to grow and photosynthetic enhancing, then
Temperature and humidity is reduced, increase illumination and nutrient solution concentration strengthen nodal bud resistance, make nodal bud sturdy, improve the light of nodal bud
Cooperation is used, and accelerates growth, meets consumption of the nodal bud growth to nutritional ingredient, then temperature is improved, and increase illumination and nutrient solution are dense
The speed of growth that nodal bud is improved there is provided sufficient illumination and nutritional ingredient is spent, the length of nodal bud can reach 4 ~ 5cm after 15 days,
Save planting cost；The morning and timing in afternoon carry out hardening to nodal bud, strengthen the environmental suitability of nodal bud, survival rate after plantation
To 100%, hence it is evident that improve the income of peasant household.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of method for culturing seedlings for improving sugarcane germination percentage, comprises the following steps：
（1）It is prepared by sugarcane kind：Selection nodal bud is full, sugarcane without disease pest, intercepts nodal bud, and nodal bud both sides sugarcane length is 1.5 ~ 2cm,
Seedling culture space is saved, is economized on resources, remaining sugarcane can carry out commercial Application, increases income, nodal bud is placed in 2 DEG C of leaching
Steep in liquid, be standing and soak for 4 hours, obtain sugarcane kind；
（2）Sugarcane kind is colonized：Field planting hole is dug on pearl cotton plate, sugarcane kind traverse is in pearl cotton plate, and nodal bud upward, is colonized,
Neat and tidy, is easy to unified management, must be colonized sugarcane kind plate；
（3）Sugarcane kind nursery：Field planting sugarcane kind plate is lain against on seedling culture frame, the vertical range between two plates is 40 ~ 45cm, is made full use of
Space is planted, planting cost is saved, nutrient solution is sprayed, rearing condition is adjusted, 1 ~ 3 day, temperature is 23 DEG C, ambient humidity is 78%,
Illumination is 80lx, adaptability of the increase nodal bud to environment, it is to avoid stronger illumination causes nodal bud to be wilted, and stimulates nodal bud to grow and light
The enhancing of cooperation, 4 ~ 9 days, temperature was 20 DEG C, ambient humidity is that 75%, illumination is 160lx, strengthens nodal bud resistance, makes nodal bud
It is sturdy, improve the photosynthesis of nodal bud, accelerate growth, meet nodal bud and grow consumption to nutritional ingredient, 10 days and after, temperature
It is that 73%, illumination is 200lx there is provided sufficient illumination and nutritional ingredient for 24 DEG C, ambient humidity, improves the speed of growth of nodal bud,
Obtain sugarcane seedling；
（4）Sugarcane seedling hardening：When sugarcane seedling length is to 4 ~ 5cm, every morning 9:30~10:30, regulation temperature is 28 DEG C, first day
Kept for 8 minutes, extend 4 minutes daily later, afternoon 4:00~5:00, regulation temperature is 14 DEG C, is kept for 6 minutes within first day, after
Extension 3 minutes, strengthen the environmental suitability of nodal bud daily, improve plantation survival rate, hence it is evident that improve the income of peasant household.
The step（1）Soak, pH be 5.7 ~ 5.9, be made up of the raw material of following parts by weight：Xanthium sibiricum 6,
In Valerian root P.E 6, aminomethylbenzoic acid 2.7, methyl jasmonate 1.6, the hot water that aminomethylbenzoic acid is added to a small amount of 70 ~ 80 DEG C, stirring
To being completely dissolved, room temperature is cooled to, xanthium sibiricum, Valerian root P.E, methyl jasmonate and normal-temperature water is added, stirring is equal
Even, the concentration for making methyl jasmonate is 5.2mmol/L, and plant extracts being capable of antibacterial bacteriostatic, promotion wound healing, it is to avoid hair
Mould, the aminomethylbenzoic acid and methyl jasmonate of addition can promote growing for nodal bud, accelerate germination, improve germination percentage, increase
The application approach of aminomethylbenzoic acid and methyl jasmonate, changes the effect that traditional methyl jasmonate suppresses rudiment, obtains soak.
Described xanthium sibiricum, is to crush the achene of Siberian cocklebur, and the volume fraction for adding 30 times of amounts of achene of Siberian cocklebur weight is 78%
Alcoholic solution, in 40 DEG C, 31kHz ultrasounds 15 minutes, filtering, the volume fractions that 50 times of amounts of filter residue weight are added into filter residue are
47% alcoholic solution, in 43 DEG C, 31kHz ultrasounds 20 minutes, it is original 1/10 that heating, which is boiled to volume, and filtering merges all
Filtrate, drying to water content is 7 ~ 9%, obtains xanthium sibiricum.
Described Valerian root P.E, valerian is crushed, and adds the water of 100 times of amounts of valerian weight, heating decocted after boiling to
Volume is original 1/20, filtering, and the alcoholic solution that volume fraction is 90% is added into filtrate, final concentration of to alcohol
45%, 12 hours are stood in 2 DEG C, filtering takes precipitation, and drying to water content is 8 ~ 10%, obtains Valerian root P.E.
Described methyl jasmonate, purity is 98 ~ 100%.
The step（3）Nutrient solution, be made up of the raw material of following parts by weight：Urea 21, calcium superphosphate 13, potassium sulfate
15th, manganese sulfate 4, thiamine 2.5, inositol 1.7, now with the current, 1 ~ 3 day, and the mass concentration of nutrient solution is 262.3mg/L, and every 2 is small
When spray 1 time, 4 ~ 9 days, mass concentration was 267.2mg/L, every 2 hours sprinkling 1 time, 10 days and after, mass concentration is
270.4mg/L, sprays 1 time for every 4 hours.
The step（4）Hardening, continuous hardening 10 days.
Embodiment 2
A kind of method for culturing seedlings for improving sugarcane germination percentage, comprises the following steps：
（1）It is prepared by sugarcane kind：Selection nodal bud is full, sugarcane without disease pest, intercepts nodal bud, and nodal bud both sides sugarcane length is 1.5 ~ 2cm,
Seedling culture space is saved, is economized on resources, remaining sugarcane can carry out commercial Application, increases income, nodal bud is placed in 3 DEG C of leaching
Steep in liquid, be standing and soak for 4 hours, obtain sugarcane kind；
（2）Sugarcane kind is colonized：Field planting hole is dug on pearl cotton plate, sugarcane kind traverse is in pearl cotton plate, and nodal bud upward, is colonized,
Neat and tidy, is easy to unified management, must be colonized sugarcane kind plate；
（3）Sugarcane kind nursery：Field planting sugarcane kind plate is lain against on seedling culture frame, the vertical range between two plates is 40 ~ 45cm, is made full use of
Space is planted, planting cost is saved, nutrient solution is sprayed, rearing condition is adjusted, 1 ~ 3 day, temperature is 24 DEG C, ambient humidity is 79%,
Illumination is 90lx, adaptability of the increase nodal bud to environment, it is to avoid stronger illumination causes nodal bud to be wilted, and stimulates nodal bud to grow and light
The enhancing of cooperation, 4 ~ 9 days, temperature was 21 DEG C, ambient humidity is that 76%, illumination is 160lx, strengthens nodal bud resistance, makes nodal bud
It is sturdy, improve the photosynthesis of nodal bud, accelerate growth, meet nodal bud and grow consumption to nutritional ingredient, 10 days and after, temperature
It is that 74%, illumination is 220lx there is provided sufficient illumination and nutritional ingredient for 25 DEG C, ambient humidity, improves the speed of growth of nodal bud,
Obtain sugarcane seedling；
（4）Sugarcane seedling hardening：When sugarcane seedling length is to 4 ~ 5cm, every morning 9:30~10:30, regulation temperature is 29 DEG C, first day
Kept for 9 minutes, extend 4 minutes daily later, afternoon 4:00~5:00, regulation temperature is 15 DEG C, is kept for 7 minutes within first day, after
Extension 3 minutes, strengthen the environmental suitability of nodal bud daily, improve plantation survival rate, hence it is evident that improve the income of peasant household.
The step（1）Soak, pH is 5.8, is made up of the raw material of following parts by weight：Xanthium sibiricum 7, valerian
In extract 7, aminomethylbenzoic acid 2.8, methyl jasmonate 1.7, the hot water that aminomethylbenzoic acid is added to a small amount of 70 ~ 80 DEG C, stir to complete
Fully dissolved, is cooled to room temperature, adds xanthium sibiricum, Valerian root P.E, methyl jasmonate and normal-temperature water, stirs,
Make methyl jasmonate concentration be 5.3mmol/L, plant extracts can antibacterial bacteriostatic, promote wound healing, it is to avoid it is mouldy, plus
The aminomethylbenzoic acid and methyl jasmonate entered can promote growing for nodal bud, accelerate germination, improve germination percentage, add ammonia first
The application approach of benzoic acid and methyl jasmonate, changes the effect that traditional methyl jasmonate suppresses rudiment, obtains soak.
Described xanthium sibiricum, is to crush the achene of Siberian cocklebur, and the volume fraction for adding 35 times of amounts of achene of Siberian cocklebur weight is 79%
Alcoholic solution, in 41 DEG C, 32kHz ultrasounds 15 minutes, filtering, the volume fractions that 55 times of amounts of filter residue weight are added into filter residue are
48% alcoholic solution, in 44 DEG C, 32kHz ultrasounds 20 minutes, it is original 1/10 that heating, which is boiled to volume, and filtering merges all
Filtrate, drying to water content is 7 ~ 9%, obtains xanthium sibiricum.
Described Valerian root P.E, valerian is crushed, and adds the water of 110 times of amounts of valerian weight, heating decocted after boiling to
Volume is original 1/20, filtering, and the alcoholic solution that volume fraction is 91% is added into filtrate, final concentration of to alcohol
46%, 13 hours are stood in 3 DEG C, filtering takes precipitation, and drying to water content is 8 ~ 10%, obtains Valerian root P.E.
Described methyl jasmonate, purity is 98 ~ 100%.
The step（3）Nutrient solution, be made up of the raw material of following parts by weight：Urea 22, calcium superphosphate 14, potassium sulfate
16th, manganese sulfate 5, thiamine 2.6, inositol 1.8, now with the current, 1 ~ 3 day, and the mass concentration of nutrient solution is 262.8mg/L, and every 2 is small
When spray 1 time, 4 ~ 9 days, mass concentration was 267.9mg/L, every 2 hours sprinkling 1 time, 10 days and after, mass concentration is
270.7mg/L, sprays 1 time for every 4 hours.
The step（4）Hardening, continuous hardening 11 days.
Embodiment 3
A kind of method for culturing seedlings for improving sugarcane germination percentage, comprises the following steps：
（1）It is prepared by sugarcane kind：Selection nodal bud is full, sugarcane without disease pest, intercepts nodal bud, and nodal bud both sides sugarcane length is 1.5 ~ 2cm,
Seedling culture space is saved, is economized on resources, remaining sugarcane can carry out commercial Application, increases income, nodal bud is placed in 4 DEG C of leaching
Steep in liquid, be standing and soak for 5 hours, obtain sugarcane kind；
（2）Sugarcane kind is colonized：Field planting hole is dug on pearl cotton plate, sugarcane kind traverse is in pearl cotton plate, and nodal bud upward, is colonized,
Neat and tidy, is easy to unified management, must be colonized sugarcane kind plate；
（3）Sugarcane kind nursery：Field planting sugarcane kind plate is lain against on seedling culture frame, the vertical range between two plates is 40 ~ 45cm, is made full use of
Space is planted, planting cost is saved, nutrient solution is sprayed, rearing condition is adjusted, 1 ~ 3 day, temperature is 25 DEG C, ambient humidity is 80%,
Illumination is 100lx, and increase nodal bud is to the adaptability of environment, it is to avoid stronger illumination causes nodal bud to be wilted, stimulate nodal bud grow and
Photosynthetic enhancing, 4 ~ 9 days, temperature was 22 DEG C, ambient humidity is that 77%, illumination is 180lx, strengthened nodal bud resistance, envoy
Bud is sturdy, improves the photosynthesis of nodal bud, accelerates growth, meets nodal bud and grows consumption to nutritional ingredient, 10 days and after, temperature
Degree is 26 DEG C, ambient humidity be 75%, illumination be 240lx there is provided sufficient illumination and nutritional ingredient, improve the growth speed of nodal bud
Degree, obtains sugarcane seedling；
（4）Sugarcane seedling hardening：When sugarcane seedling length is to 4 ~ 5cm, every morning 9:30~10:30, regulation temperature is 30 DEG C, first day
Kept for 10 minutes, extend 5 minutes daily later, afternoon 4:00~5:00, regulation temperature is 16 DEG C, is kept for 8 minutes within first day, with
Extend 4 minutes daily afterwards, strengthen the environmental suitability of nodal bud, improve plantation survival rate, hence it is evident that improve the income of peasant household.
The step（1）Soak, pH is 5.9, is made up of the raw material of following parts by weight：Xanthium sibiricum 8, valerian
In extract 8, aminomethylbenzoic acid 2.9, methyl jasmonate 1.8, the hot water that aminomethylbenzoic acid is added to a small amount of 70 ~ 80 DEG C, stir to complete
Fully dissolved, is cooled to room temperature, adds xanthium sibiricum, Valerian root P.E, methyl jasmonate and normal-temperature water, stirs,
Make methyl jasmonate concentration be 5.4mmol/L, plant extracts can antibacterial bacteriostatic, promote wound healing, it is to avoid it is mouldy, plus
The aminomethylbenzoic acid and methyl jasmonate entered can promote growing for nodal bud, accelerate germination, improve germination percentage, add ammonia first
The application approach of benzoic acid and methyl jasmonate, changes the effect that traditional methyl jasmonate suppresses rudiment, obtains soak.
Described xanthium sibiricum, is to crush the achene of Siberian cocklebur, and the volume fraction for adding 40 times of amounts of achene of Siberian cocklebur weight is 80%
Alcoholic solution, in 42 DEG C, 33kHz ultrasounds 20 minutes, filtering, the volume fractions that 60 times of amounts of filter residue weight are added into filter residue are
49% alcoholic solution, in 45 DEG C, 33kHz ultrasounds 25 minutes, it is original 1/10 that heating, which is boiled to volume, and filtering merges all
Filtrate, drying to water content is 7 ~ 9%, obtains xanthium sibiricum.
Described Valerian root P.E, valerian is crushed, and adds the water of 120 times of amounts of valerian weight, heating decocted after boiling to
Volume is original 1/20, filtering, and the alcoholic solution that volume fraction is 92% is added into filtrate, final concentration of to alcohol
47%, 14 hours are stood in 4 DEG C, filtering takes precipitation, and drying to water content is 8 ~ 10%, obtains Valerian root P.E.
Described methyl jasmonate, purity is 98 ~ 100%.
The step（3）Nutrient solution, be made up of the raw material of following parts by weight：Urea 23, calcium superphosphate 15, potassium sulfate
17th, manganese sulfate 6, thiamine 2.7, inositol 1.9, now with the current, 1 ~ 3 day, and the mass concentration of nutrient solution is 263.7mg/L, and every 2 is small
When spray 1 time, 4 ~ 9 days, mass concentration was 268.6mg/L, every 2 hours sprinkling 1 time, 10 days and after, mass concentration is
271.8mg/L, sprays 1 time for every 4 hours.
The step（4）Hardening, continuous hardening 12 days.
Comparative example 1
Removal step（1）In soak, remaining method, be the same as Example 1.
Comparative example 2
Remove the aminomethylbenzoic acid in soak, remaining method, be the same as Example 1.
Comparative example 3
Remove the methyl jasmonate in soak, remaining method, be the same as Example 1.
Comparative example 4
Step（2）In pearl cotton be changed to conventional soil, remaining method, be the same as Example 1.
Comparative example 5
Step（3）In rearing condition be always maintained at temperature be 23 DEG C, ambient humidity be that 78%, illumination is 80lx, the matter of nutrient solution
Amount concentration is 262.3mg/L, remaining method, be the same as Example 1.
Comparative example 6
The sugarcane stem soil method for culturing seedlings of existing conventional sugarcane.
The nursery effect of embodiment and comparative example sugarcane method for culturing seedlings：
In Anhui Province Bengbu Region, the full black sugarcane of selection nodal bud intercepts nodal bud 9000, is randomly divided into 9 groups, every group 1000
Individual, respectively embodiment group and comparative example group, each group carry out nursery according to the method for the group respectively, and other management methods are homogeneous
Together, planted after nursery, the strain that survives of seedling is observed in plantation after 15 days, calculate survival rate, embodiment and comparative example sugarcane educate
The nursery effect of seedling-growing method is shown in Table 1.
Table 1：The nursery effect of embodiment and comparative example sugarcane method for culturing seedlings
| Project | Nodal bud（It is individual） | Germination（It is individual） | Germination percentage（%） | Survive（Strain） | Survival rate（%） |
| Embodiment 1 | 1000 | 832 | 83.2 | 832 | 100.0 |
| Embodiment 2 | 1000 | 843 | 84.3 | 838 | 99.4 |
| Embodiment 3 | 1000 | 837 | 83.7 | 829 | 99.0 |
| Comparative example 1 | 1000 | 755 | 75.5 | 733 | 97.1 |
| Comparative example 2 | 1000 | 783 | 78.3 | 771 | 98.5 |
| Comparative example 3 | 1000 | 762 | 76.2 | 757 | 99.3 |
| Comparative example 4 | 1000 | 814 | 81.4 | 803 | 98.6 |
| Comparative example 5 | 1000 | 747 | 74.7 | 728 | 97.5 |
| Comparative example 6 | 1000 | 621 | 62.1 | 598 | 96.3 |
Show from the result of table 1, the method for culturing seedlings of the raising sugarcane germination percentage of embodiment, germination percentage and survival rate are substantially relatively contrasted
Example is high, illustrates the method for culturing seedlings for the raising sugarcane germination percentage that the present invention is provided and has good nursery effect.
Claims (7)
1. a kind of method for culturing seedlings for improving sugarcane germination percentage, it is characterised in that comprise the following steps：
（1）It is prepared by sugarcane kind：Selection nodal bud is full, sugarcane without disease pest, intercepts nodal bud, and nodal bud both sides sugarcane length is 2 ~ 3cm, will
Nodal bud is placed in 2 ~ 4 DEG C of soak, is standing and soak for 4 ~ 5 hours, is obtained sugarcane kind；
（2）Sugarcane kind is colonized：Field planting hole is dug on pearl cotton plate, sugarcane kind traverse is in pearl cotton plate, and nodal bud upward, is colonized,
Sugarcane kind plate must be colonized；
（3）Sugarcane kind nursery：Field planting sugarcane kind plate is lain against on seedling culture frame, the vertical range between two plates is 40 ~ 45cm, sprays nutrition
Liquid, adjusts rearing condition, 1 ~ 3 day, temperature be 23 ~ 25 DEG C, ambient humidity is that 78 ~ 80%, illumination is 80 ~ 100lx, 4 ~ 9 days, warm
It is that 75 ~ 77%, illumination is 160 ~ 180lx to spend for 20 ~ 22 DEG C, ambient humidity, 10 days and after, temperature is 24 ~ 26 DEG C, environmental wet
It is 200 ~ 240lx to spend for 73 ~ 75%, illumination, obtains sugarcane seedling；
（4）Sugarcane seedling hardening：When sugarcane seedling length is to 4 ~ 5cm, every morning 9:30~10:30, regulation temperature is 28 ~ 30 DEG C, first
It is kept for 8 ~ 10 minutes, extends 4 ~ 5 minutes daily later, afternoon 4:00~5:00, regulation temperature is 14 ~ 16 DEG C, is kept within first day
6 ~ 8 minutes, extend 3 ~ 4 minutes daily later.
2. the method for culturing seedlings of sugarcane germination percentage is improved according to claim 1, it is characterised in that the step（1）Immersion
Liquid, pH is 5.7 ~ 5.9, is made up of the raw material of following parts by weight：Xanthium sibiricum 6 ~ 8, Valerian root P.E 6 ~ 8, aminomethylbenzoic acid
2.7 ~ 2.9, in methyl jasmonate 1.6 ~ 1.8, the hot water that aminomethylbenzoic acid is added to a small amount of 70 ~ 80 DEG C, stir to being completely dissolved, it is cold
But to room temperature, xanthium sibiricum, Valerian root P.E, methyl jasmonate and normal-temperature water is added, is stirred, make jasmonic first
The concentration of ester is 5.2 ~ 5.4mmol/L, obtains soak.
3. the method for culturing seedlings of sugarcane germination percentage is improved according to claim 2, it is characterised in that the described achene of Siberian cocklebur is extracted
Thing, is to crush the achene of Siberian cocklebur, and the volume fraction for adding 30 ~ 40 times of amounts of achene of Siberian cocklebur weight is 78 ~ 80% alcoholic solution, in 40 ~ 42
DEG C, 31 ~ 33kHz ultrasounds 15 ~ 20 minutes, filtering, the volume fraction that 50 ~ 60 times of amounts of filter residue weight are added into filter residue is 47 ~ 49%
Alcoholic solution, in 43 ~ 45 DEG C, 31 ~ 33kHz ultrasounds 20 ~ 25 minutes, it is original 1/10 that heating, which is boiled to volume, filters, closes
And all filtrates, drying to water content is 7 ~ 9%, obtains xanthium sibiricum.
4. the method for culturing seedlings of sugarcane germination percentage is improved according to claim 2, it is characterised in that described Valerian root P.E,
Valerian is crushed, the water of 100 ~ 120 times of amounts of valerian weight is added, it is original 1/20 that heating, which is decocted after boiling to volume, filtering,
The alcoholic solution that volume fraction is 90 ~ 92% is added into filtrate, to final concentration of the 45 ~ 47% of alcohol, stand 12 in 2 ~ 4 DEG C ~
14 hours, filtering took precipitation, and drying to water content is 8 ~ 10%, obtains Valerian root P.E.
5. the method for culturing seedlings of sugarcane germination percentage is improved according to claim 2, it is characterised in that described methyl jasmonate,
Purity is 98 ~ 100%.
6. the method for culturing seedlings of sugarcane germination percentage is improved according to claim 1, it is characterised in that the step（3）Nutrition
Liquid, is made up of the raw material of following parts by weight：Urea 21 ~ 23, calcium superphosphate 13 ~ 15, potassium sulfate 15 ~ 17, manganese sulfate 4 ~ 6, thiamines
Element 2.5 ~ 2.7, inositol 1.7 ~ 1.9, now with the current, 1 ~ 3 day, the mass concentration of nutrient solution was 262.3 ~ 263.7mg/L, and every 2 is small
When spray 1 time, 4 ~ 9 days, mass concentration be 267.2 ~ 268.6mg/L, every 2 hours sprinkling 1 time, 10 days and after, mass concentration
For 270.4 ~ 271.8mg/L, spray 1 time within every 4 hours.
7. the method for culturing seedlings of sugarcane germination percentage is improved according to claim 1, it is characterised in that the step（4）Hardening,
Continuous hardening 10 ~ 12 days.
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| CN108886907A (en) * | 2018-08-08 | 2018-11-27 | 广西科技师范学院 | A method of promoting sugarcane rudiment |
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| CN102696371A (en) * | 2012-05-23 | 2012-10-03 | 雷选 | Sugarcane node bud seedling raising and cultivating method |
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| CN101642050A (en) * | 2009-08-21 | 2010-02-10 | 广州甘蔗糖业研究所 | Method for enhancing survival rate of grafting sugarcane group seedling |
| CN102498912A (en) * | 2011-11-23 | 2012-06-20 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
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