CN103503775A - Sugarcane tissue culture seedling potassium nutrition research method - Google Patents
Sugarcane tissue culture seedling potassium nutrition research method Download PDFInfo
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- CN103503775A CN103503775A CN201310467648.5A CN201310467648A CN103503775A CN 103503775 A CN103503775 A CN 103503775A CN 201310467648 A CN201310467648 A CN 201310467648A CN 103503775 A CN103503775 A CN 103503775A
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Abstract
The invention discloses a sugarcane tissue culture seedling potassium nutrition research method. The method comprises the steps: preparing four culture mediums K1, K2, K3 and K4 with different potassium levels; transplanting tissue culture seedlings with not promoted roots of different sugarcane varieties ROC22, YT55 and YT60 in the culture mediums of test tubes, wherein one seedling is transplanted in one test tube, every variety is finished for 4 times corresponding to K1, K2, K3 and K4, and every finishing is repeated for 6 times; sealing the test tubes by using ventilation sealing films, placing the test tubes wrapped with black films at the culture medium parts in an illumination culture room, setting the temperature as 26 DEG C, the illuminance as 40001x, illumination time is 12 h/day and humidity as 80%, and implementing the culture for 30-45 days; testing the growth index and the potassium content of the cultivated tissue culture seedlings; implementing the data difference significance analysis for the test results. The method can obtain the demands of the tissue culture seedlings of the different sugarcane varieties to the potassium element, so as to provide the basis for cultivating strong sugarcane seedlings.
Description
Technical field
The present invention relates to grow seedling nutritional research field, particularly relate to a kind of sugar-cane tissue culture seedlings potassium nutrition research method.
Background technology
Sugarcane is the most important sugar [yielding of China, and its output accounts for national sugar total amount more than 85%, and therefore, the plantation of sugarcane has very important significance for China's economic development and social stability.Sugarcane is C4 crop, has larger biomass, causes it to being in great demand of nutrient element, the especially demand to potassium element.The research of sugarcane nutrient demand seems particularly important to improving its biomass.
But the sugarcane production cycle is long, the field growing time, the ，Shi Qi field nutrient test that affects of the environmental factors such as climate, soil was difficult to obtain accurate result approximately about 12 months, and often field trial need to expend the plenty of time, man power and material.
At present, studying the demand of different sugar cane breeds to nutrient, is to cultivate urgently one of problem to be solved of healthy and strong cane seedling.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of sugar-cane tissue culture seedlings potassium nutrition research method, can show that the group of different sugar cane breeds is trained the demand of seedling to potassium element, for cultivating healthy and strong cane seedling, provides foundation.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of sugar-cane tissue culture seedlings potassium nutrition research method is provided, comprises step:
Medium K1, K2, K3 and the K4 of 4 kinds of different potassium levels of configuration;
K1, K2, K3 and K4 medium are divided and installed in teat glass on superclean bench, each cuvette cartridge 30ml, wherein, the medium of each potassium level arranges 6 pipes and repeats;
The not short root group training seedling of different sugar cane breed ROC22, YT55 and YT60 is transplanted in the medium of test tube on superclean bench, each test tube one strain, each kind corresponding K1, K2, K3 and K4 carry out 4 times to be processed, and each is processed and repeats 6 times;
After transplanting completes, with breathable sealing film, test tube is sealed and tightened;
The test tube of sealing is all positioned on rack for test tube, and utilizes black thin film that the medium of test tube is partly wrapped up so that the group training shoot root in medium is shading growth;
The rack for test tube wrapping is placed in to illumination cultivation chamber, and the temperature of setting illumination cultivation chamber is 26 ℃, and illuminance is 4000lx, and light application time is 12h/ days, and humidity is 80%, cultivates 30-45 days;
Medium on group training seedling after cultivating is cleaned, with pure water rinsing 2 times, after suck dry moisture, measured fresh weight, plant height and utilize root scanner to carry out root system scanning, and add up long, the total radical of root, root surface area and root volume;
The baking oven that group training seedling after above-mentioned measurement is placed in to 70 ℃ is dried to constant weight, grinds after measuring its dry weight, carries out nitrogen, phosphorus, potassium content and measure after the group training seedling powder grinding adopts sulfuric acid-hydrogen peroxide to disappear to boil;
To above-mentioned every measurement result, adopt SAS or SPSS analysis software to carry out the significance of difference analysis of data.
The invention has the beneficial effects as follows: the situation that is different from prior art, the present invention is by arranging medium K1, K2, K3 and the K4 of 4 kinds of different potassium levels, and the not short root group training seedling of 3 different sugar cane breed ROC22, YT55 and YT60 is cultivated on the medium arranging, the corresponding 4 kinds of medium of each kind carry out 4 times to be processed, and each is processed and repeats 6 times.Cultivate after 30-45 days, the biological indicators such as the fresh weight of mensuration group training seedling, root length and nitrogen, phosphorus, potassium content, and index is carried out to statistical analysis.By said method, can show that the group of different sugar cane breeds is trained the demand of seedling to potassium element, for cultivating healthy and strong cane seedling, provide foundation.
Embodiment
Embodiment 1
Sugar-cane tissue culture seedlings is the most effectual way that obtains sugarcane detoxication seedling, in sugarcane tissue culture, medium is the unique channel that group training seedling obtains nutrient, various compositions in medium provide required nutrient for sugar-cane tissue culture seedlings, its composition and the required nutrient of field sugarcane production are basically identical, be all the essential various mineral elements of plant growth, comprise macroelement, in, trace element etc.Different cultivars sugar-cane tissue culture seedlings is different to nutrient demand, and research sugar-cane tissue culture seedlings can provide reference frame for cultivating healthy and strong group training seedling to the difference of nutrient demand.
The sugar-cane tissue culture seedlings potassium nutrition research method of the present embodiment is as follows:
A. configure medium K1, K2, K3 and the K4 of 4 kinds of different potassium levels.
Concrete configuration method is: according to different potassium level formulas, the composition of MS medium and ratio are regulated, comprise the following steps:
A. macroelement is configured to separately to mother liquor, as: potassium nitrate, ammonium nitrate, nitrate of lime, magnesium sulfate and calcium chloride are all configured to 1mol/L mother liquor, and calcium dihydrogen phosphate is configured to 0.05mol/L mother liquor, and after having configured, sterilizing is stand-by;
B. macroelement mother liquor configuration being completed mixes by different potassium levels, and dilutes 1000 times;
C. in the mother liquor after dilution, add trace element and organic principle, sterilizing is stand-by.
Wherein, the potassium level of medium K1 is 0mmol/L, and the formula of configuration 1L medium K1 is:
Macroelement:
Trace element:
Organic principle:
Sucrose: 30g/L
Agar: 7g/L
The potassium level of medium K2 is 0.3mmol/L, and the formula of configuration 1L medium K2 is: macroelement:
Wherein, medium K2 is identical with the content of trace element in K1, organic principle.
The potassium level of medium K3 is 0.75mmol/L, and the formula of configuration 1L medium K3 is: macroelement:
Wherein, medium K3 is identical with the content of trace element in K1, organic principle.
The potassium level of medium K4 is 1.5mmol/L, and the formula of configuration 1L medium K4 is: macroelement:
Wherein, medium K4 is identical with the content of trace element in K1, organic principle.
B. the medium preparing is divided while hot and installed in teat glass on superclean bench, each cuvette cartridge 30ml, cooling stand-by, wherein, the medium of each potassium level arranges 6 pipes and repeats.
In the present embodiment, the length of teat glass is 30cm, and diameter is 3cm.Now, test tube and medium can be weighed for statistical analysis.
C. by the new platform of different sugar cane breeds sugar 22(ROC22), Guangdong sugar 55(YT55) and Guangdong sugar 60(YT60) short root group train seedling and on superclean bench, be transplanted in the medium of test tube, each test tube one strain, each kind corresponding K1, K2, K3 and K4 carry out 4 times to be processed, and each is processed and repeats 6 times.
In the present embodiment, the genotype of different cultivars ROC22, YT55 and YT60 is different, and the subculture number of its not short root group training seedling is no more than 3 times.
Be specially with tweezers cultured not short root group training seedling is transplanted in the medium of test tube, each test tube one strain, each kind corresponding K1, K2, K3 and K4 carry out 4 times to be processed, and each is processed and repeats 6 times, amounts to 72 pipes.
D., after having transplanted, with breathable sealing film, test tube is sealed and tightened.
E. the test tube of sealing is all positioned on rack for test tube, and utilizes black thin film parcel.
The black thin film parcel for medium part of test tube, can make the group training shoot root in medium is shading growth, avoids the impact of illumination on group training seedling root growth.
F. the rack for test tube wrapping is placed in to illumination cultivation chamber, the temperature of setting illumination cultivation chamber is 26 ℃, and illuminance is 4000lx, and light application time is 12h/ days, and humidity is 80%, cultivates 30-45 days.
Wherein, incubation time can be determined according to the upgrowth situation of group training seedling, and the group training seedling that is specially different disposal is grown while there is difference.
G. the medium on group training seedling after cultivating is cleaned, with pure water rinsing 2 times, after suck dry moisture, measured fresh weight, plant height and utilize root scanner to carry out root system scanning, and add up long, the total radical of root, root surface area and root volume.
In the present embodiment, cultivate after 45 days, carry out above-mentioned growth indexes mensuration.
H. pack the group training seedling after above-mentioned measurement into envelope and be placed in the baking oven of 70 ℃ and dry to constant weight, grind after measuring its dry weight, after the group training seedling grinding adopts sulfuric acid-hydrogen peroxide to disappear to boil, carry out nitrogen, phosphorus, potassium content and measure.
In the present embodiment, can utilize small-sized plant cracker that the group training seedling after drying is ground, mixed.
Wherein, nitrogen content and the phosphorus content of group training seedling are used Flow Analyzer to measure, and potassium content is used flame spectrophotometer measuring.
I. to above-mentioned every measurement result, adopt SAS or SPSS analysis software to carry out the significance of difference analysis of data.
Said determination data utilize Excell to collect, and then utilize analysis software analysis.Analysis result can compare the difference of different genotype group training seedling under same potassium level is processed, also can the difference of more a certain genotype group training seedling under different potassium level conditions.
In the present embodiment, utilize SAS10 software to carry out statistical analysis the data obtained, analysis result is, under K1, K2 condition, the biomass of ROC22, root length, root volume, root surface area are all significantly lower than YT55 and YT60, under the low-down condition of explanation potassium content in medium, the YT55 that is not so good as of ROC22 growth goes with YT60.
Along with the rising of K1-K4 potassium level, the biomass of each genotype group training seedling is all increasing gradually, illustrates that potassium is very important to the growth of group training seedling.But different genotype biomass changes significant difference, as: the amplitude of variation of ROC22 biomass is very large, and YT55 and YT60 amplitude of variation are relatively little, illustrate that ROC22 is more responsive to potassium.
Above-mentioned analysis result has very important significance to absorbing of potassium for research different genotype sugar-cane tissue culture seedlings, for cultivating sugarcane health seedling, also has certain guiding value.
In sum, the advantages such as sugar-cane tissue culture seedlings potassium nutrition research method of the present invention has strong operability, the cycle is short, nutrient Regulation is convenient, accurate, contribute to select medium nutrient content according to different sugar cane breeds, thereby cultivate more healthy and stronger cane seedling, be conducive to the nutritional need characteristic of different genotype sugar-cane tissue culture seedlings to be understood in depth simultaneously.
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes specification of the present invention to do, or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (8)
1. a sugar-cane tissue culture seedlings potassium nutrition research method, is characterized in that, comprises step:
Medium K1, K2, K3 and the K4 of 4 kinds of different potassium levels of configuration;
Described K1, K2, K3 and K4 medium are divided and installed in teat glass on superclean bench, each cuvette cartridge 30ml, wherein, the medium of each potassium level arranges 6 pipes and repeats;
The not short root group training seedling of different sugar cane breed ROC22, YT55 and YT60 is transplanted in the medium of described test tube on superclean bench, each test tube one strain, each kind corresponding K1, K2, K3 and K4 carry out 4 times to be processed, and each is processed and repeats 6 times;
After transplanting completes, with breathable sealing film, test tube is sealed and tightened;
The test tube of sealing is all positioned on rack for test tube, and utilizes black thin film that the medium of test tube is partly wrapped up so that the group training shoot root in medium is shading growth;
The rack for test tube wrapping is placed in to illumination cultivation chamber, and the temperature of setting illumination cultivation chamber is 26 ℃, and illuminance is 4000lx, and light application time is 12h/ days, and humidity is 80%, cultivates 30-45 days;
Medium on group training seedling after cultivating is cleaned, with pure water rinsing 2 times, after suck dry moisture, measured fresh weight, plant height and utilize root scanner to carry out root system scanning, and add up long, the total radical of root, root surface area and root volume;
The baking oven that group training seedling after above-mentioned measurement is placed in to 70 ℃ is dried to constant weight, grinds after measuring its dry weight, carries out nitrogen, phosphorus, potassium content and measure after the group training seedling powder grinding adopts sulfuric acid-hydrogen peroxide to disappear to boil;
To above-mentioned every measurement result, adopt SAS or SPSS analysis software to carry out the significance of difference analysis of data.
2. method according to claim 1, is characterized in that, the collocation method of described medium K1, K2, K3 or K4 comprises the following steps:
Macroelement is configured to separately to mother liquor, as: potassium nitrate, ammonium nitrate, nitrate of lime, magnesium sulfate and calcium chloride are all configured to 1mol/L mother liquor, and calcium dihydrogen phosphate is configured to 0.05mol/L mother liquor, and after having configured, sterilizing is stand-by;
The macroelement mother liquor that configuration is completed mixes by different potassium levels, and dilutes 1000 times;
In mother liquor after dilution, add trace element and organic principle, sterilizing, completes the configuration of medium.
3. method according to claim 2, is characterized in that, the potassium level of described medium K1 is 0mmol/L, and wherein, the formula of configuration 1L medium K1 is:
Macroelement:
Trace element:
Organic principle:
Sucrose: 30g/L
Agar: 7g/L.
4. method according to claim 3, is characterized in that, the potassium level of described medium K2 is 0.3mmol/L, and wherein, the formula of configuration 1L medium K2 is:
Macroelement:
Wherein, described medium K2 is identical with the content of trace element in K1, organic principle.
5. method according to claim 4, is characterized in that, the potassium level of described medium K3 is 0.75mmol/L, and wherein, the formula of configuration 1L medium K3 is:
Macroelement:
Wherein, described medium K3 is identical with the content of trace element in K1, organic principle.
6. method according to claim 5, is characterized in that, the potassium level of described medium K4 is 1.5mmol/L, and wherein, the formula of configuration 1L medium K4 is:
Macroelement:
Wherein, described medium K4 is identical with the content of trace element in K1, organic principle.
7. method according to claim 6, is characterized in that, the subculture number of the not short root group training seedling of described different sugar cane breed ROC22, YT55 and YT60 is no more than 3 times.
8. method according to claim 7, is characterized in that, the nitrogen content of described group of training seedling and phosphorus content are used Flow Analyzer to measure, and potassium content is used flame spectrophotometer measuring.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542262A (en) * | 2015-01-28 | 2015-04-29 | 云南省农业科学院甘蔗研究所 | Method for increasing maturing rate in sugarcane hybrid seed production |
| CN111089777A (en) * | 2020-01-20 | 2020-05-01 | 云南省烟草质量监督检测站 | Method for rapidly determining potassium content in organic fertilizer for tobacco |
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| US20030219896A1 (en) * | 1997-02-21 | 2003-11-27 | Abdelrahman Layla Zakaria | Sugar cane production |
| CN101642050A (en) * | 2009-08-21 | 2010-02-10 | 广州甘蔗糖业研究所 | Method for enhancing survival rate of grafting sugarcane group seedling |
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2013
- 2013-10-09 CN CN201310467648.5A patent/CN103503775A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030219896A1 (en) * | 1997-02-21 | 2003-11-27 | Abdelrahman Layla Zakaria | Sugar cane production |
| CN101642050A (en) * | 2009-08-21 | 2010-02-10 | 广州甘蔗糖业研究所 | Method for enhancing survival rate of grafting sugarcane group seedling |
Non-Patent Citations (2)
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| 陈迪文等: "不同基因型甘蔗组培苗钾营养特性研究", 《中国农学通报》, vol. 29, no. 22, 5 August 2013 (2013-08-05) * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542262A (en) * | 2015-01-28 | 2015-04-29 | 云南省农业科学院甘蔗研究所 | Method for increasing maturing rate in sugarcane hybrid seed production |
| CN104542262B (en) * | 2015-01-28 | 2017-02-22 | 云南省农业科学院甘蔗研究所 | Method for increasing maturing rate in sugarcane hybrid seed production |
| CN111089777A (en) * | 2020-01-20 | 2020-05-01 | 云南省烟草质量监督检测站 | Method for rapidly determining potassium content in organic fertilizer for tobacco |
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Application publication date: 20140115 |