CN102187773A - Cultivation method for improving salt tolerance of eggplant by applying external source NO to root - Google Patents
Cultivation method for improving salt tolerance of eggplant by applying external source NO to root Download PDFInfo
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- CN102187773A CN102187773A CN2010101171714A CN201010117171A CN102187773A CN 102187773 A CN102187773 A CN 102187773A CN 2010101171714 A CN2010101171714 A CN 2010101171714A CN 201010117171 A CN201010117171 A CN 201010117171A CN 102187773 A CN102187773 A CN 102187773A
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- eggplant
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- snp
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Abstract
The invention discloses a cultivation method for improving salt tolerance of an eggplant, which is characterized in that: the salt-tolerant eggplant is realized by a method of applying an external source NO donor of sodium nitroprusside (Sodium nitroprusside, SNP) to the root under water culture conditions. On the basis of applying the external source NO at different concentrations to the root, strong seedlings which are high in salt resistance and high growth potential in the period of young seedling are obtained by a method of selecting the optimal NO concentration. The external source NO donor of SNP is provided by the Sigma Company and has the optimal concentration of 0.1mmol.L<-1>; and the eggplant is a low-salt-tolerance facility variety. The cultivation form is a water culture form. The problems of lower salt tolerance of the facility eggplant and lower growth potential in the period of seedlings are effectively solved by applying the external source NO to the root.
Description
One, technical field:
The present invention relates to improve the culture technique of eggplant salt resistance, specifically is a kind ofly to execute the culture technique that exogenous NO gas is improved the eggplant salt resistance by root.
Two, background technology:
The greenhouse soil secondary salinization is a ubiquitous technical barrier in the domestic and international facility cultivation.Salt damage is more and more serious to the threat that eggplant causes, and causes its yield and quality to descend, and has a strong impact on the sustainable development that its facility is produced.In the method that improves the eggplant salt resistance, it is exactly a kind of effective method that root is executed exogenous NO gas.Eggplant (Solanum melongena L.) is a kind of vegetable crop to the medium sensitivity of salinity, under the salt marsh condition eggplant grow and output can be had a strong impact on.So, be badly in need of a kind ofly promptly can improving the eggplant salt resistance, be again a kind ofly to alleviate crop salt adverse circumstance injury, adapt to adverse circumstance and improve the effective ways of stress resistance,, enrich plant stress physiology theory simultaneously for the improvement and the resistance breeding of eggplant culture technique provides theoretical foundation.
Three, summary of the invention:
The present invention proposes a kind ofly to execute the method that allogenic material NO improves facility eggplant salt resistance by root.The present invention is achieved in that and a kind ofly executes exogenous NO gas by root and improve eggplant salt resistance cultivation method, it is characterized in that: described eggplant salt resistance enhancing is to execute exogenous NO gas donor SNP through root to realize, the exogenous NO gas of the variable concentrations for preparing is handled the eggplant seedling under the water planting condition salt stress, and filtering out optium concentration is 0.1mmolL
-1Exogenous NO gas donor SNP is provided by Sigma company; Eggplant is the more weak facility cultivation kind of salt resistance.Used planting form is the water planting cultivation.Execute the exogenous NO gas means by root and effectively solved the more weak problem of facility eggplant salt resistance.
The invention has the beneficial effects as follows: execute eggplant with the exogenous NO gas root, except improving its salt resistance greatly, because exogenous NO gas is less to the influence report of eggplant seedling physiological property under the salt stress, so not only improved the eggplant salt resistance, provide theoretical foundation in the hope of high-quality and efficient cultivation and sustainable development for eggplant, and by studying NO to the various Physiological Mechanism in Regulation of plant under the salt stress, disclose function and the mechanism of action of NO in plant, further further investigate the effect of NO in the plant abiotic stress.
Four, embodiment:
1. the cultivation method of this raising facility eggplant salt resistance is to execute the exogenous NO gas technology by root to realize.Should take into full account the condition such as method, the time interval of compound method, preparation time, the processing of exogenous NO gas.
This patent when preparation exogenous NO gas concentration, use ten thousand/ the balance weighing, prepare 100mmolL with distilled water earlier
-1Mother liquor, 4 ℃ of preservations, the time spent by the experiment required concentration dilute; In order to guarantee the stability of concentration of treatment, executed one time exogenous NO gas during the processing in per 2 days.By the eggplant root under the salt stress is executed different exogenous NO gas concentration, we find 0.1mmolL
-1It is the strongest that SNP improves the eggplant salt resistance.To the various Physiological Mechanism in Regulation of eggplant under the salt stress, further disclose function and the mechanism of action of NO in plant by research NO; And execute exogenous NO gas by root and improve the eggplant salt resistance, method is simple.
2. management when test is handled
Each must dissolve fully by NaCl when changing nutrient solution, pour the SNP solution for preparing into Turnover Box after, Turnover Box is constantly stirred, it is mixed.
In the process of electric air pump ventilation, because the secretion of eggplant root system or other impurity may stop up the ventilation pump, so every day will be to the inspection of ventilation pump, so that the normal growth of eggplant.Other management method is consistent with conventional method.
Embodiment:
One, exogenous NO gas is to 100mmolL
-1NaCl coerces down the influence of eggplant seedling
1 materials and methods
1.1 material
Eggplant (Solanummelongena L.) kind for examination is ' 08-9 ', is provided by the Academy of Agricultural Sciences, Shanghai City.
1.2 method
1.2.1 the examination material is cultivated
Test is carried out in Shanghai academy of agricultural sciences horticulture institute greenhouse.With seed presoaking and germinating, be sowed at respectively after sprouting in the plastics nutritive cube of diameter 10cm, high 10cm, vermiculite is made matrix.True leaf launches the every 2d in back and waters 1/8 concentration Japan garden examination nutrient solution once; When seedling has 4～5 true leaves, after selecting the consistent plant of growth and cleaning root matrix, to transplant in the plastic box of long 47cm, wide 34cm, high 12cm, every case is contained the 12L nutrient solution, and every case is planted 6 strains, tries nutrient solution with the Japanese garden of 1/8 concentration and cultivates.Change 1/4 concentration nutrient solution after 1 week into, after this per 4 days replacing one time of nutrition liquid.Ventilated continuously in 24 hours with electric air pump during the nutrient fluid cultivation.
1.2.2SNP solution preparation
NO donor sodium nitroprusside (sodium nitroprusside, SNP is available from Shanghai traditional Chinese medicines group company) is prepared 100mmolL with distilled water earlier
-1Mother liquor, 4 ℃ of preservations, the time spent by the experiment required concentration dilute.
1.2.3 test is handled
When plant has 6～7 true leaves, with containing 100mmolL
-11/4 concentration of NaCl Japan garden examination nutrient solution carries out salt stress to be handled, and adds the variable concentrations sodium nitroprussiate simultaneously in solution, and change nutrient solution every day during the processing.If (seeing Table 1) handled in 5 kinds of tests, every processing 6 strains, 3 repetitions, randomized arrangement in the greenhouse being handled the mensuration that every index is carried out in sampling in the 10th day.
Table 1 test is handled and numbering
1.2.4 plant strain growth situation
Measure that average plant height, stem are thick, overground part fresh weight and root system fresh weight.
1.2.5 physiological index determining
Measure the antioxidant enzyme (SOD, POD, CAT and APX) of blade, active oxygen ROS content (MDA and O
2Produce speed), photosynthetic rate etc.
2. result
2.1 biomass changes
Compare 0.05～0.2mmolL with S1
-1SNP handles the biomass of seedling under salt stress is increased, and the influence that variable concentrations SNP handles the tomato seedling biomass accumulation there are differences, wherein with SNP 0.1mmolL
-1It is best to handle (S3) effect.
2.2ROS (MDA and O
2Produce speed)
Compare with S1, the SNP processing of variable concentrations all can significantly reduce NaCl and coerce down MDA content and O
2Rate of release is wherein minimum with S2 and S3 content.
2.3 polyphenoils enzymic activity
The activity that SNP handles eggplant seedling blade SOD, POD, CAT and APX has appreciable impact, increase along with SNP concentration, 4 kinds of enzymic activitys all are the back downward trend that raises earlier, show that SNP has dose effect to the influence of the above-mentioned 4 kinds of enzymic activitys of eggplant seedling blade, low concentration promotes enzymic activity, high concentration inhibitory enzyme activity.
2.5 gas exchange parameter
Compare with S1, SNP handles eggplant seedling blade Pn, Gs is improved, wherein with SNP 0.1mmolL
-1It is best to handle (S3) effect.
The component and the concentration of nutrient solution see the following form
Claims (3)
1. cultivation method that utilizes allogenic material to improve the eggplant salt resistance is characterized in that: described salt resistance eggplant is to realize that through the method that root is executed exogenous NO gas water planting condition salt is used variable concentrations (0.05,0.1 and 0.2 μ molL under handling
-1) exogenous NO gas, 0.1mmolL wherein
-1Best to the eggplant effect under the salt stress.
2. the cultivation method of salt resistance eggplant according to claim 1 is characterized in that: 0.1mmolL
-1SNP is best to the eggplant effect under the water planting condition salt stress, and eggplant is the more weak facility kind of salt resistance.
3. the cultivation method of raising eggplant salt resistance according to claim 1 and 2 is characterized in that: used planting form is the water planting cultivation, and selected optimum N O concentration is only used in eggplant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101171714A CN102187773A (en) | 2010-03-04 | 2010-03-04 | Cultivation method for improving salt tolerance of eggplant by applying external source NO to root |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101171714A CN102187773A (en) | 2010-03-04 | 2010-03-04 | Cultivation method for improving salt tolerance of eggplant by applying external source NO to root |
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| CN102187773A true CN102187773A (en) | 2011-09-21 |
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| CN2010101171714A Pending CN102187773A (en) | 2010-03-04 | 2010-03-04 | Cultivation method for improving salt tolerance of eggplant by applying external source NO to root |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355348A (en) * | 2013-07-30 | 2013-10-23 | 中国热带农业科学院橡胶研究所 | Plant cold resistant protective agent |
| CN103650890A (en) * | 2013-12-24 | 2014-03-26 | 河南师范大学 | Method for improving yield of peanuts |
| US11330774B2 (en) | 2015-12-24 | 2022-05-17 | Sekisui Chemical Co., Ltd. | Hydroponic culture method for plant in high salinity environment |
-
2010
- 2010-03-04 CN CN2010101171714A patent/CN102187773A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355348A (en) * | 2013-07-30 | 2013-10-23 | 中国热带农业科学院橡胶研究所 | Plant cold resistant protective agent |
| CN103355348B (en) * | 2013-07-30 | 2014-12-10 | 中国热带农业科学院橡胶研究所 | Application process of plant cold resistant protective agent |
| CN103650890A (en) * | 2013-12-24 | 2014-03-26 | 河南师范大学 | Method for improving yield of peanuts |
| CN103650890B (en) * | 2013-12-24 | 2016-01-20 | 河南师范大学 | A kind of method improving peanut yield |
| US11330774B2 (en) | 2015-12-24 | 2022-05-17 | Sekisui Chemical Co., Ltd. | Hydroponic culture method for plant in high salinity environment |
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Application publication date: 20110921 |