CN102939943A - Artificial propagation method for myzus persicae - Google Patents
Artificial propagation method for myzus persicae Download PDFInfo
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- CN102939943A CN102939943A CN2012104282101A CN201210428210A CN102939943A CN 102939943 A CN102939943 A CN 102939943A CN 2012104282101 A CN2012104282101 A CN 2012104282101A CN 201210428210 A CN201210428210 A CN 201210428210A CN 102939943 A CN102939943 A CN 102939943A
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Abstract
The invention particularly relates to an artificial propagation method for myzus persicae, which belongs to the technical fields of insect breeding and propagation. The method comprises the steps of choosing a myzus persicae sensing tobacco variety, raising seedlings, transplanting, choosing myzus persicae, inoculating the myzus persicae and propagating the myzus persicae. According to the artificial propagation method, the propagation time and the propagation quantity of the myzus persicae can be adjusted flexibly according to practical requirements of tests or researches; different requirements of the myzus persicae under different conditions are met; and the method is simple to operate and uses little time and few labors.
Description
Technical field
The invention belongs to insect captive breeding technical field, specifically, relate to the artificial fecundation method of a kind of cigarette aphid.
Background technology
The cigarette aphid claims again black peach aphid, is worldwide insect, generally occurs and causes harm serious in China.Cigarette aphid host plant nearly more than 300 is planted, and its happiness food host comprises the multiple forests such as the various vegetables such as tobacco, potato, Chinese cabbage, rape, wild cabbage and peach.The causing harm of cigarette aphid comprises directly causes harm and two aspects that indirectly cause harm, and mainly refers to into the aphid thorn and inhales plant juice and affect the growth of plant normal growth if directly cause harm; Indirectly cause harm mainly and to refer to that it as passing malicious medium, can propagate the various plants virus disease.
But the cigarette aphid is a kind of test material commonly used, and the life that the can be used as pesticide species screening material of measuring and monitoring the growth of standing timber is also through being usually used in the entomology correlative study.
For a long time, in the correlative study of cigarette aphid, assurance is for the quantity of trying the worm source, quality and availability is particularly important for conducting a research smoothly at any time.The at present supply for examination worm source mainly is by the field collection or utilizes conventional potted plant host plant to breed in the greenhouse.But because the seasonality of host plant growth clearly, the cigarette aphid is in the impact that is subject to the each side factors such as weather, environment of host's season of growth, and the field collecting method is bred and is difficult to guarantee for the uniformity of examination cigarette aphid and the requirement of quantity.Need a large amount of flowerpots, Nutrition Soil and breed the cigarette aphid the conventional potted plant host plant of greenhouse utilization, take up room large, need to often water, recruitment is more, in case the host plant lack of water is easy to cause wilting, affects aphid breeding quantity.
Therefore in the urgent need to researching and developing a kind of method that is suitable for the artificial propagation of cigarette aphid, to satisfy the needs of correlative study.
Summary of the invention
For solving the problems of the technologies described above, the present invention proposes a kind of simple to operate and cigarette aphid artificial fecundation method that output cigarette aphid uniformity is higher.
Technical scheme of the present invention is: the artificial fecundation method of cigarette aphid, and concrete steps are as follows:
(1) cultivates the host tobacco
A, selection sense aphid tobacco bred: select the tobacco bred of high sense cigarette aphid for subsequent use;
B, grow seedlings: with 15～30 ℃ of temperature, humidity 60～70%, with the greenhouse of fly net or warm canopy as seedling rearing room, in seedling rearing room, tobacco seed educated when growing up to 5～6 true leaves for subsequent use;
C, transplanting: the tobacco strain that step b breeds is transplanted to nutrient solution, tobacco root is immersed in the nutrient solution;
(2) cigarette aphid breeding
A, plant aphid and select: pick up from the tobacco land for growing field crops, select individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid;
B, connect aphid: 20～25 ℃ of temperature, relative moisture 65%～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours, greenhouse or the conduct of warm canopy with fly net connect the aphid chamber, involve in a criminal case to move into together with nutrient solution and connect the aphid chamber transplanting, cultivate cigarette after 5～6 days, 5～10 of the aptery one-tenth aphids of every strain cigarette strain access;
C, numerous aphid: 20～25 ℃ of temperature, relative moisture 65%～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours,, involved in a criminal case with nutrient solution and moved into together numerous aphid chamber connecing cigarette behind the aphid as numerous aphid chamber with the greenhouse of fly net or warm canopy, freely breed, after the cigarette strain is covered with aphid, change the cigarette strain, repeating step a-c;
When breeding the cigarette aphid for the second time, plant aphid and directly in numerous aphid chamber, choose, and then repeat to connect aphid, numerous aphid step.
Among described step (1)-b, the seedling-cultivating method of described tobacco is floating seedlings method, husky training seedling method or moistening seedling method.
Among described step (1)-c, during transplanting, get the brown wide-mouth bottle of bore 2～3cm, height 7～8cm, add nutrient solution, the circular sponge of clip thin layer, the sponge slightly larger in diameter is in the wide-mouth bottle bore, cut off to home position along radius, cigarette strain root soil is cleaned, cane is clipped in the sponge home position, place wide-mouth bottle, cigarette strain root system is immersed in the nutrient solution.
In the described step (2), described kind of aphid picked up from the tobacco land for growing field crops, select individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid.
The component of described nutrient solution and percentage by weight are: potassium nitrate 1%～3%, nitrate of lime 0.05%～0.07%, ammonium dihydrogen phosphate (ADP) 1.0%～1.5%, ferrous sulfate 0.005%～0.006%, magnesium sulfate 1.5%～2.0%, boric acid 0.01%～0.03%, manganese sulphate 0.01%～0.02%, copper sulphate 0.0001%～0.0003%, zinc sulphate 0.0001%～0.0003%, deionized water surplus.
The preparation process of described nutrient solution is as follows:
(1) according to the gross weight of the nutrient solution of required preparation, calculates the weight of required component by the percentage by weight of said components.
(2) preparation I liquid: dissolve ferrous sulfate with warm water, then dissolve nitrate of lime, the limit adds the waterside stirring until dissolve fully, is settled to 1/2 weight of required preparation nutrient solution with water, is mixed with I liquid;
(3) preparation II liquid: dissolve first magnesium sulfate, then add successively ammonium dihydrogen phosphate (ADP), potassium nitrate, add water and be stirred to fully dissolving, boric acid adds so that the warm water dissolving is rear, add again manganese sulphate, copper sulphate, zinc sulphate, be settled to 1/2 weight of required preparation nutrient solution with water.
(4) preparation nutrient solution: get 0.5%～1.5% of I liquid weight, mix with II liquid again, be settled at last the nutrient solution weight of required preparation with water.
Beneficial effect of the present invention is:
1, is not subjected to the impact in season, can raises throughout the year;
2, the aphis population breeding is fast, and a breeding generation approximately needs 5 days, than reducing 1～2 day under the natural conditions, can use worm by guarantee test;
3, Reproduction Conditions is stable, easily obtains the unified test of standard and uses worm;
4, recruitment is less, is easy to management, and simple to operate;
5, raise aphid with respect to the potted plant host plant of routine, occupy little space.
Embodiment
Specify the present invention below in conjunction with embodiment.
Embodiment 1
The artificial fecundation method of cigarette aphid, concrete steps are as follows:
(1) cultivates the host tobacco
A, selection sense aphid tobacco bred: select sense worm kind G-28 for subsequent use.
B, grow seedlings: with 15～30 ℃ of temperature, humidity 60～70%, with the greenhouse of fly net or warm canopy as seedling rearing room, in seedling rearing room, tobacco seed educated when growing up to 5～6 true leaves for subsequent use.
The seedling-cultivating method of tobacco is the conventional seedbed system method, for example floating seedlings method, husky training seedling method or moistening seedling method.
C, transplanting: the tobacco strain that step b breeds is transplanted to nutrient solution, tobacco root is immersed in the nutrient solution.Transplant altogether 10 strain cigarette seedlings.
During transplanting, get the brown wide-mouth bottle of bore 2～3cm, height 7～8cm, add nutrient solution, the circular sponge of clip thin layer, the sponge slightly larger in diameter is cut off to home position along radius in the wide-mouth bottle bore, cigarette strain root soil is cleaned, cane is clipped in the sponge home position, places wide-mouth bottle, and cigarette strain root system is immersed in the nutrient solution.
(2) cigarette aphid breeding
A, plant aphid and select: select to pick up from tobacco land for growing field crops, Jimo City, Shandong, individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid;
B, connect aphid: 20～25 ℃ of temperature, relative moisture 65～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours, greenhouse or the conduct of warm canopy with fly net connect the aphid chamber, involve in a criminal case to move into together with nutrient solution and connect the aphid chamber transplanting, cultivate cigarette after 5～6 days, 5 of the aptery one-tenth aphids of every strain cigarette strain access;
C, numerous aphid: 20～25 ℃ of temperature, relative moisture 65～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours,, involve in a criminal case with nutrient solution and move into together numerous aphid chamber connecing cigarette behind the aphid as numerous aphid chamber with the greenhouse of fly net or warm canopy, freely breed, after the cigarette strain is covered with aphid, change cigarette strain repeating step a-c;
When breeding the cigarette aphid for the second time, plant aphid and directly in numerous aphid chamber, choose, and then repeat to connect aphid, numerous aphid step.
The component of the nutrient solution that uses in the above-mentioned steps and weight are: potassium nitrate 40g, nitrate of lime 120g, ammonium dihydrogen phosphate (ADP) 28g, ferrous sulfate 12g, magnesium sulfate 35g, boric acid 0.5g, manganese sulphate 0.4g, copper sulphate 0.004g, zinc sulphate 0.004g.
As follows by said components and weight preparation nutrient solution step:
(1) preparation I liquid: dissolve ferrous sulfate with warm water, then dissolve nitrate of lime, the limit adds the waterside stirring until fully dissolving is settled to 1000mL with deionized water;
(2) preparation II liquid: dissolve first magnesium sulfate, then add successively ammonium dihydrogen phosphate (ADP), potassium nitrate, add water and be stirred to fully dissolving, boric acid adds so that the warm water dissolving is rear, adds at last other component, is settled to 1000mL with deionized water.
(3) the preparation nutrient solution measures first I liquid 10mL and is dissolved in the 1000mL deionized water, mixes getting final product with II liquid again.
Embodiment 2
Outside lower difference, other are with embodiment 1.
The tobacco bred of selecting is cloud and mist 87;
The kind aphid of selecting be pick up from Sichuan Liangshan District tobacco land for growing field crops, individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid.
Embodiment 3
Outside lower difference, other are with embodiment 1.
The tobacco bred of selecting is ZC-01;
The kind aphid of selecting be the tobacco land for growing field crops of picking up from Zhucheng, individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid.
Comparative Examples 1
Outside lower difference, other are with embodiment 1.
Cigarette aphid artificial fecundation method, concrete steps are as follows:
(1) cultivates the host tobacco
A, selection sense aphid tobacco bred: select sense worm kind G-28 for subsequent use.
B, grow seedlings: with 15～30 ℃ of temperature, humidity 60～70%, with the greenhouse of fly net or warm canopy as seedling rearing room, in seedling rearing room, tobacco seed educated when growing up to 5～6 true leaves for subsequent use.
The seedling-cultivating method of tobacco is the conventional seedbed system method, for example floating seedlings method, husky training seedling method or moistening seedling method.
C, transplanting: the tobacco strain that step b breeds is transplanted to pot experiment, adopt the flowerpot of diameter 15cm, in-built Nutrition Soil is transplanted 10 strain cigarette seedlings altogether.
(2) cigarette aphid breeding
A, plant aphid and select: select to pick up from Shandong Jimo vega, individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid.
B, connect aphid: 20～25 ℃ of temperature, relative moisture 65～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours, greenhouse or the conduct of warm canopy with fly net connect the aphid chamber, involve in a criminal case to move into together with flowerpot and connect the aphid chamber transplanting, cultivate cigarette after 5～6 days, 5 of the aptery one-tenth aphids of every strain cigarette strain access;
C, numerous aphid: 20～25 ℃ of temperature, relative moisture 65～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours,, involve in a criminal case with flowerpot and move into together numerous aphid chamber connecing cigarette behind the aphid as numerous aphid chamber with the greenhouse of fly net or warm canopy, freely breed, after the cigarette strain is covered with aphid, change cigarette strain repeating step a-c;
When breeding the cigarette aphid for the second time, plant aphid and directly in numerous aphid chamber, choose, and then repeat to connect aphid, numerous aphid step.As kind of an aphid;
Comparative Examples 2
Outside lower difference, other are with Comparative Examples 1.
The tobacco bred of selecting is cloud and mist 87;
The kind aphid of selecting be pick up from Sichuan Liangshan District tobacco land for growing field crops, individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid.
Comparative Examples 3
Outside lower difference, other are with Comparative Examples 1.
The tobacco bred of selecting is ZC-01;
The kind aphid of selecting be the tobacco land for growing field crops of picking up from Zhucheng, individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid.
Check experiment
After freely breeding 10 days respectively according to the method for embodiment 1～3, Comparative Examples 1～3, the quantity of calculating cigarette aphid, experimental data sees Table 1.
| Tobacco bred | Plant the aphid source | Breed cigarette aphid quantity after 10 days | |
| Embodiment 1 | G-28 | Shandong Jimo vega | 2850 |
| Comparative Examples 1 | G-28 | Shandong Jimo vega | 1578 |
| Embodiment 2 | Cloud and mist 87 | The Sichuan Liangshan District vega | 3025 |
| Comparative Examples 2 | Cloud and mist 87 | The Sichuan Liangshan District vega | 2532 |
| Embodiment 3 | ZC-01 | The Zhucheng vega | 2765 |
| Comparative Examples 3 | ZC-01 | The Zhucheng vega | 1432 |
The cigarette aphid quantity that table 1: embodiment 1～3, Comparative Examples 1～3 obtain
As can be seen from Table 1, the Aphed population that adopts propagation method of the present invention to obtain is significantly higher than potted plant host plant propagation method, and aphis population quantity is 1～2 times of potted plant host plant breeding; Method of the present invention has been saved the space, and 10 strain cigarette seedlings approximately take up an area 0.06m
2, and potted plant host plant propagation method need be taken up an area 0.15m
2
Claims (4)
1. the artificial fecundation method of cigarette aphid, concrete steps are as follows:
(1) cultivates the host tobacco
A, selection sense aphid tobacco bred: the height sense cigarette aphid tobacco bred of selection is for subsequent use;
B, grow seedlings: with 15～30 ℃ of temperature, humidity 60%～70%, with the greenhouse of fly net or warm canopy as seedling rearing room, in seedling rearing room, tobacco seed educated when growing up to 5～6 true leaves for subsequent use;
C, transplanting: the tobacco strain that step b breeds is transplanted to nutrient solution, tobacco root is immersed in the nutrient solution;
(2) cigarette aphid breeding
A, plant aphid and select: select individual large, vitality by force, not by the cigarette aphid of pathogen or parasitic wasp parasitism as kind of an aphid;
B, connect aphid: 20～25 ℃ of temperature, relative moisture 65%～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours, greenhouse or the conduct of warm canopy with fly net connect the aphid chamber, involve in a criminal case to move into together with nutrient solution and connect the aphid chamber transplanting, cultivate cigarette after 5～6 days, 5～10 of the aptery one-tenth aphids of every strain cigarette strain access;
C, numerous aphid: 20～25 ℃ of temperature, relative moisture 65%～75%, use fluorescent lamp lighting, every day, light application time was 13～15 hours,, involve in a criminal case with nutrient solution and move into together numerous aphid chamber connecing cigarette behind the aphid as numerous aphid chamber with the greenhouse of fly net or warm canopy, freely breed, after the cigarette strain is covered with aphid, change cigarette strain repeating step a-c;
When breeding the cigarette aphid for the second time, plant aphid and directly in numerous aphid chamber, choose.
2. the artificial fecundation method of cigarette aphid according to claim 1 is characterized in that, among described step (1)-b, the seedling-cultivating method of described tobacco is floating seedlings method, husky training seedling method or moistening seedling method.
3. the artificial fecundation method of cigarette aphid according to claim 1 is characterized in that, among described step (1)-c, during transplanting, get the brown wide-mouth bottle of bore 2～3cm, height 7～8cm, add nutrient solution, the circular sponge of clip thin layer, the sponge slightly larger in diameter is in the wide-mouth bottle bore, cut off to home position along radius, cigarette strain root soil is cleaned, cane is clipped in the sponge home position, place wide-mouth bottle, cigarette strain root system is immersed in the nutrient solution.
4. according to claim 1 or the artificial fecundation method of 3 described cigarette aphids, it is characterized in that, the component of described nutrient solution and concentration are: potassium nitrate 1%～3%, nitrate of lime 0.05%～0.07%, ammonium dihydrogen phosphate (ADP) 1.0%～1.5%, ferrous sulfate 0.005%～0.006%, magnesium sulfate 1.5%～2.0%, boric acid 0.01%～0.03%, manganese sulphate 0.01%～0.02%, copper sulphate 0.0001%～0.0003%, zinc sulphate 0.0001%～0.0003%, deionized water surplus.
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Cited By (8)
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| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN103503836A (en) * | 2013-08-22 | 2014-01-15 | 北京市农林科学院 | Plutella xylostella cotesia rubecula artificial propagation production method |
| CN103960009A (en) * | 2014-04-08 | 2014-08-06 | 山西省农业科学院小麦研究所 | Wheat yellow dwarf disease nature resisting phenotype identification method |
| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
| CN106417192A (en) * | 2016-10-25 | 2017-02-22 | 福建省烟草公司三明市公司 | Low-humidity myzus persicae host crop breeding device |
| CN108157104A (en) * | 2017-12-12 | 2018-06-15 | 贵州省烟草公司黔东南州公司 | A kind of breeding method of aphid parasite conservation and expanding species cigarette strain |
| CN109479835A (en) * | 2019-01-18 | 2019-03-19 | 河南省农业科学院烟草研究所 | A kind of reproductive capacity identification method of cigarette aphid on different tobacco breds |
| CN114097712A (en) * | 2021-11-24 | 2022-03-01 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Royal aphidiidae breeding device and method |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103503836A (en) * | 2013-08-22 | 2014-01-15 | 北京市农林科学院 | Plutella xylostella cotesia rubecula artificial propagation production method |
| CN103478083B (en) * | 2013-09-29 | 2015-03-11 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN103960009A (en) * | 2014-04-08 | 2014-08-06 | 山西省农业科学院小麦研究所 | Wheat yellow dwarf disease nature resisting phenotype identification method |
| CN103960009B (en) * | 2014-04-08 | 2015-08-12 | 山西省农业科学院小麦研究所 | One grow wheat resistance to yellow dwarf phenotypic evaluation method |
| CN105519496B (en) * | 2016-02-16 | 2017-11-21 | 弥渡格瑞生物科技有限责任公司 | A kind of natural diffuseness of cigarette aphid connects aphid method |
| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
| CN106417192A (en) * | 2016-10-25 | 2017-02-22 | 福建省烟草公司三明市公司 | Low-humidity myzus persicae host crop breeding device |
| CN106417192B (en) * | 2016-10-25 | 2022-02-22 | 福建省烟草公司三明市公司 | Low-humidity myzus persicae host crop breeding device |
| CN108157104A (en) * | 2017-12-12 | 2018-06-15 | 贵州省烟草公司黔东南州公司 | A kind of breeding method of aphid parasite conservation and expanding species cigarette strain |
| CN108157104B (en) * | 2017-12-12 | 2020-08-04 | 贵州省烟草公司黔东南州公司 | Method for cultivating tobacco plants for protecting and expanding aphidiidae |
| CN109479835A (en) * | 2019-01-18 | 2019-03-19 | 河南省农业科学院烟草研究所 | A kind of reproductive capacity identification method of cigarette aphid on different tobacco breds |
| CN114097712A (en) * | 2021-11-24 | 2022-03-01 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Royal aphidiidae breeding device and method |
| CN114097712B (en) * | 2021-11-24 | 2023-08-15 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Device and method for breeding aphidius gifuensis |
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Application publication date: 20130227 |