CN103430767B - The medium of gastrodia elata sexual propagation Germination Strain three grades of kinds and breeding method - Google Patents
The medium of gastrodia elata sexual propagation Germination Strain three grades of kinds and breeding method Download PDFInfo
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Abstract
The breeding method of gastrodia elata sexual propagation Germination Strain disclosed by the invention three grades of kinds, comprise get the raw materials ready, soak, draining, spice, pack, covering, sterilizing, cooling, access secondary mother plant, cultivate totally 10 steps, solves miscellaneous bacteria serious interference in existing gastrodia elata sexual propagation Germination Strain cultivating process and the complicated problem of formula.The invention also discloses the medium of a kind of gastrodia elata sexual propagation Germination Strain three grades of kinds, be made up of following raw material: Qinggang ebon leaf of 72% ~ 74%, the wood chip of 26% ~ 28%, the quality sum of above each component is 100%.The growth that this medium is Germination Strain at Germination Strain early growth period wood chip provides enough nutrients, improve Germination Strain survival rate, along with the growth of Germination Strain, Qinggang ebon leaf contains enough nutrients, can for a long time, be continuously Germination Strain growth nutrient is provided, ensure Germination Strain grow fast, simultaneously, Qinggang ebon leaf obtains easily, and fresh Qinggang ebon leaf miscellaneous bacteria is grown few, can effectively reduce the invasion and attack of miscellaneous bacteria in Germination Strain incubation.
Description
Technical field
The invention belongs to method of breeding strain technical field, be specifically related to the breeding method of a kind of gastrodia elata sexual propagation Germination Strain three grades of kinds, the invention still further relates to the medium of a kind of gastrodia elata sexual propagation Germination Strain three grades of kinds.
Background technology
Rhizoma Gastrodiae is herbaceos perennial, is distributed in China's most area.Its dry tuber commonly uses and famousr and precious Chinese medicine simply, is clinically used for the disease such as headache dizzy, extremity numbness, child convulsion, epilepsy, tic, tetanus.The acquisition of rhizoma Gastrodiae relies on wild resource in the past always, and the 20 wild change men of century 70 plant successfully, and rhizoma Gastrodiae is planted by family becomes staple source.Rhizoma Gastrodiae is chosen as by World Conservation Union species of easily endangering; and be put in the appendix II of " CITES "; also be put in China's " national key protected wild plants register (second batch) " simultaneously; being II grade of protective plant, is one of primary raw material for the treatment of cardiovascular and cerebrovascular diseases medicament.
Rhizoma Gastrodiae is born in the more and moistening sylvan life of humus, and shrubbery and careless slope also have on the sunny side.Rhizoma Gastrodiae is generally cultivated in China, distributed more widely, and rhizoma Gastrodiae main product is planted in Southern Shaanxi, Northern Sichuan Province, West of Hubei Province and other places by family.Rhizoma Gastrodiae adopts stem tuber to breed usually, but owing to affecting by miscellaneous bacteria, there will be by for degradation phenomena, yields poorly and unstable, affect rhizoma Gastrodiae production greatly.The another kind of method of producing rhizoma Gastrodiae adopts seed sexual propagation exactly, but under natural conditions, seeds of Gastrodia elata sexual propagation germination rate is low, is only 1%.Gastrodia elata sexual propagation seed germination must rely on Germination Strain supply nutrition, can improve seeds of Gastrodia elata germination rate under the effect of Germination Strain, and is conducive to forming rhizoma Gastrodiae ball stem, and is grown to serve as common Gastrodia Elata Stem Tuber.Domestic explored gastrodia elata seed Germination bacterium has Mycen osmundicola, dendrobium mixture, Mycena orchidicola and Mycena anoectochila), and wherein dendrobium mixture is applied more in rhizoma Gastrodiae artificial planting.
At present in the cultivating process of gastrodia elata sexual propagation Germination Strain, the blank that normal employing saw dust, white sugar, wheat bran, starch etc. mix is as medium, this powder-mixed medium is usually because miscellaneous bacteria is attacked and moisture is wayward too much, affect the growth of Germination Strain, and culture base-material complicated component, technical process is loaded down with trivial details, and cost of manufacture is higher.
Patent name: the medium of tall gastrodia tuber germination strains, the applying date: 2010.11.04, publication number: CN102153377A, publication date: 2011.08.17, disclose a kind of culture medium prescription of tall gastrodia tuber germination strains, this medium adopts Qingmu Tree fruit to be primary raw material, mix with wheat bran, sawdust, sugar, starch, this base-material increases substantially although can make gastrodia seed germination rate have, but there is the acquisition of Qingmu Tree fruit to be subject to seasonal restrictions, can not pluck at any time, not easily obtain, the shortcoming of base material formulation complexity.
Summary of the invention
The object of the present invention is to provide the breeding method of a kind of gastrodia elata sexual propagation Germination Strain three grades of kinds, solve the problem of miscellaneous bacteria serious interference and formula complexity in existing gastrodia elata sexual propagation Germination Strain cultivating process.
Another object of the present invention is the medium providing a kind of gastrodia elata sexual propagation Germination Strain three grades of kinds, solves existing medium base material formulation complicated, the problem that cost is high.
The technical solution adopted in the present invention is: the breeding method of gastrodia elata sexual propagation Germination Strain three grades of kinds, comprises the steps:
The first step, gets the raw materials ready
Pluck Qinggang ebon leaf, and dry for subsequent use;
Second step, soaks
Qinggang ebon leaf in the first step is placed in clear water to soak 20 ~ 30 hours;
3rd step, draining
Pull Qinggang ebon leaf that second step is obtained out, be placed in dry place draining, make water content in the ebon leaf of Qinggang be 65% ~ 70%;
4th step, spice
Mix through Qinggang ebon leaf of the 3rd step process according to the ratio that mass ratio is 4:1 with the wood chip of water content 62% ~ 68%, and stir, obtained compound;
5th step, pack
Loaded in strain bag by compound obtained for 4th step, charge weight is 80% ~ 82% of strain bag capacity;
6th step, covers
The remaining space of the strain bag of the 5th step is filled wood chip, and wood chip is covered in the top layer of described compound;
7th step, sterilizing
Strain bag through the 6th step process is put into sterilizing chamber, and the temperature of sterilizing chamber is set to 124 ~ 125 DEG C, and the pressure of sterilizing chamber is set to 0.14 ~ 0.15MPa, carries out sterilization treatment 3 hours;
8th step, cooling
Strain bag through the 7th step process is put into temperature not higher than the indoor of 18 DEG C, cools 3 ~ 5 hours, make the temperature of strain bag not higher than 20 DEG C;
9th step, access secondary mother plants
In an aseptic environment by gastrodia elata sexual propagation Germination Strain original seed access strain bag;
Tenth step, cultivates
Strain bag through the 9th step process being positioned over temperature is cultivate 40 ~ 50 days in the culturing room of 20 ~ 24 DEG C, obtained gastrodia elata sexual propagation Germination Strain three grades of kinds.
Feature of the present invention is also,
Gastrodia elata sexual propagation Germination Strain original seed in 9th step is the one in Mycen osmundicola, dendrobium mixture, Mycena orchidicola or Mycena anoectochila).
Another technical scheme of the present invention is: the medium of gastrodia elata sexual propagation Germination Strain three grades of kinds, is made up of following raw material according to mass percent: Qinggang ebon leaf of 72% ~ 76%, the wood chip of 24% ~ 28%, and the quality sum of above each component is 100%.
Feature of the present invention is also,
The water content of Qinggang ebon leaf is 65% ~ 70%.
The water content of wood chip is 62% ~ 68%.
The invention has the beneficial effects as follows: the breeding method of gastrodia elata sexual propagation Germination Strain of the present invention three grades of kinds adopts Qinggang ebon leaf as the primary raw material of culture base-material, Qinggang ebon leaf is mixed with wood chip, form culture base-material, the growth being Germination Strain at Germination Strain early growth period wood chip provides enough nutrients, improve Germination Strain survival rate, along with the growth of Germination Strain, Qinggang ebon leaf contains enough nutrients, can be long-time, be continuously Germination Strain growth and nutrient is provided, ensure that Germination Strain grows fast, simultaneously, Qinggang ebon leaf obtains easily, and fresh Qinggang ebon leaf miscellaneous bacteria is grown few, the invasion and attack of miscellaneous bacteria in Germination Strain incubation can be effectively reduced.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
The medium of gastrodia elata sexual propagation Germination Strain three grades of kinds, is made up of following raw material according to mass percent: Qinggang ebon leaf of 72% ~ 76%, the wood chip of 24% ~ 28%, and the quality sum of above each component is 100%.Wherein wood chip is the obtained wood chip of the timber of any seeds, the acquisition of Qinggang ebon leaf and wood chip is all very easy, and the compositing formula of medium is simple, and cost is low, and the miscellaneous bacteria of Qinggang ebon leaf is grown few, is extremely conducive to the cultivation of gastrodia elata sexual propagation Germination Strain.
Embodiment 1
The breeding method of gastrodia elata sexual propagation Germination Strain three grades of kinds, comprises the steps:
The first step, gets the raw materials ready
Pluck Qinggang ebon leaf, and dry for subsequent use;
Second step, soaks
Qinggang ebon leaf in the first step is placed in clear water to soak 20 hours;
3rd step, draining
Pull Qinggang ebon leaf that second step is obtained out, be placed in dry place draining, make water content in the ebon leaf of Qinggang be 65%;
4th step, spice
Mix through Qinggang ebon leaf of the 3rd step process according to the ratio that mass ratio is 4:1 with the wood chip of water content 62%, and stir, obtained compound;
5th step, pack
Loaded in strain bag by compound obtained for 4th step, charge weight is 80% of strain bag capacity;
6th step, covers
The remaining space of the strain bag of the 5th step is filled wood chip, and wood chip is covered in the top layer of compound, obtained medium, in medium containing mass fraction be 72% Qinggang ebon leaf and mass fraction be the wood chip of 28%;
7th step, sterilizing
Strain bag through the 6th step process is put into sterilizing chamber, and the temperature of sterilizing chamber is set to 124 DEG C, and the pressure of sterilizing chamber is set to 0.14MPa, carries out sterilization treatment 3 hours;
8th step, cooling
Strain bag through the 7th step process is put into temperature not higher than the indoor of 18 DEG C, cools 3 hours, make the temperature of strain bag not higher than 20 DEG C;
9th step, access secondary mother plants
In an aseptic environment by dendrobium mixture original seed access strain bag;
Tenth step, cultivates
Strain bag through the 9th step process being positioned over temperature is cultivate 40 days in the culturing room of 20 DEG C, obtained gastrodia elata sexual propagation Germination Strain three grades of kinds.
Gastrodia elata sexual propagation Germination Strain three grades of kinds that the present embodiment is obtained, make rhizoma Gastrodiae germination rate more than 80%, and substantially eliminate the interference of miscellaneous bacteria in gastrodia elata sexual propagation Germination Strain process of growth, make the growth in rhizoma Gastrodiae later stage seldom occur random root, improve the output of rhizoma Gastrodiae.
Embodiment 2
The breeding method of gastrodia elata sexual propagation Germination Strain three grades of kinds, comprises the steps:
The first step, gets the raw materials ready
Pluck Qinggang ebon leaf, and dry for subsequent use;
Second step, soaks
Qinggang ebon leaf in the first step is placed in clear water to soak 30 hours;
3rd step, draining
Pull Qinggang ebon leaf that second step is obtained out, be placed in dry place draining, make water content in the ebon leaf of Qinggang be 70%;
4th step, spice
Mix through Qinggang ebon leaf of the 3rd step process according to the ratio that mass ratio is 4:1 with the wood chip of water content 68%, and stir, obtained compound;
5th step, pack
Loaded in strain bag by compound obtained for 4th step, charge weight is 82% of strain bag capacity;
6th step, covers
The remaining space of the strain bag of the 5th step is filled wood chip, and wood chip is covered in the top layer of compound, obtained medium, in medium containing mass fraction be 76% Qinggang ebon leaf and mass fraction be the wood chip of 24%;
7th step, sterilizing
Strain bag through the 6th step process is put into sterilizing chamber, and the temperature of sterilizing chamber is set to 125 DEG C, and the pressure of sterilizing chamber is set to 0.15MPa, carries out sterilization treatment 3 hours;
8th step, cooling
Strain bag through the 7th step process is put into temperature not higher than the indoor of 18 DEG C, cools 5 hours, make the temperature of strain bag not higher than 20 DEG C;
9th step, access secondary mother plants
In an aseptic environment by Mycen osmundicola original seed access strain bag;
Tenth step, cultivates
Strain bag through the 9th step process being positioned over temperature is cultivate 50 days in the culturing room of 24 DEG C, obtained gastrodia elata sexual propagation Germination Strain three grades of kinds.
Gastrodia elata sexual propagation Germination Strain three grades of kinds that the present embodiment is obtained, make rhizoma Gastrodiae germination rate more than 83%, and substantially eliminate the interference of miscellaneous bacteria in gastrodia elata sexual propagation Germination Strain process of growth, make the growth in rhizoma Gastrodiae later stage seldom occur random root, improve the output of rhizoma Gastrodiae.
Embodiment 3
The breeding method of gastrodia elata sexual propagation Germination Strain three grades of kinds, comprises the steps:
The first step, gets the raw materials ready
Pluck Qinggang ebon leaf, and dry for subsequent use;
Second step, soaks
Qinggang ebon leaf in the first step is placed in clear water to soak 25 hours;
3rd step, draining
Pull Qinggang ebon leaf that second step is obtained out, be placed in dry place draining, make water content in the ebon leaf of Qinggang be 68%;
4th step, spice
Mix through Qinggang ebon leaf of the 3rd step process according to the ratio that mass ratio is 4:1 with the wood chip of water content 65%, and stir, obtained compound;
5th step, pack
Loaded in strain bag by compound obtained for 4th step, charge weight is 81% of strain bag capacity;
6th step, covers
The remaining space of the strain bag of the 5th step is filled wood chip, and wood chip is covered in the top layer of compound, obtained medium, in medium containing mass fraction be 73% Qinggang ebon leaf and mass fraction be the wood chip of 27%;
7th step, sterilizing
Strain bag through the 6th step process is put into sterilizing chamber, and the temperature of sterilizing chamber is set to 124.5 DEG C, and the pressure of sterilizing chamber is set to 0.145MPa, carries out sterilization treatment 3 hours;
8th step, cooling
Strain bag through the 7th step process is put into temperature not higher than the indoor of 18 DEG C, cools 4 hours, make the temperature of strain bag not higher than 20 DEG C;
9th step, access secondary mother plants
In an aseptic environment by Mycena orchidicola original seed access strain bag;
Tenth step, cultivates
Strain bag through the 9th step process being positioned over temperature is cultivate 45 days in the culturing room of 22 DEG C, obtained gastrodia elata sexual propagation Germination Strain three grades of kinds.
Gastrodia elata sexual propagation Germination Strain three grades of kinds that the present embodiment is obtained, make rhizoma Gastrodiae germination rate more than 85%, and substantially eliminate the interference of miscellaneous bacteria in gastrodia elata sexual propagation Germination Strain process of growth, make the growth in rhizoma Gastrodiae later stage seldom occur random root, improve the output of rhizoma Gastrodiae.
Embodiment 4
The breeding method of gastrodia elata sexual propagation Germination Strain three grades of kinds, comprises the steps:
The first step, gets the raw materials ready
Pluck Qinggang ebon leaf, and dry for subsequent use;
Second step, soaks
Qinggang ebon leaf in the first step is placed in clear water to soak 28 hours;
3rd step, draining
Pull Qinggang ebon leaf that second step is obtained out, be placed in dry place draining, make water content in the ebon leaf of Qinggang be 66%;
4th step, spice
Mix through Qinggang ebon leaf of the 3rd step process according to the ratio that mass ratio is 4:1 with the wood chip of water content 62%, and stir, obtained compound;
5th step, pack
Loaded in strain bag by compound obtained for 4th step, charge weight is 80% of strain bag capacity;
6th step, covers
The remaining space of the strain bag of the 5th step is filled wood chip, and wood chip is covered in the top layer of compound, obtained medium, in medium containing mass fraction be 75% Qinggang ebon leaf and mass fraction be the wood chip of 25%;
7th step, sterilizing
Strain bag through the 6th step process is put into sterilizing chamber, and the temperature of sterilizing chamber is set to 124 DEG C, and the pressure of sterilizing chamber is set to 0.15MPa, carries out sterilization treatment 3 hours;
8th step, cooling
Strain bag through the 7th step process is put into temperature not higher than the indoor of 18 DEG C, cools 4 hours, make the temperature of strain bag not higher than 20 DEG C;
9th step, access secondary mother plants
In an aseptic environment by Mycena anoectochila) original seed access strain bag;
Tenth step, cultivates
Strain bag through the 9th step process being positioned over temperature is cultivate 45 days in the culturing room of 22 DEG C, obtained gastrodia elata sexual propagation Germination Strain three grades of kinds.
Gastrodia elata sexual propagation Germination Strain three grades of kinds that the present embodiment is obtained, make rhizoma Gastrodiae germination rate more than 87%, and substantially eliminate the interference of miscellaneous bacteria in gastrodia elata sexual propagation Germination Strain process of growth, make the growth in rhizoma Gastrodiae later stage seldom occur random root, improve the output of rhizoma Gastrodiae.
Claims (1)
1. the breeding method of gastrodia elata sexual propagation Germination Strain three grades of kinds, it is characterized in that, have employed the medium of gastrodia elata sexual propagation Germination Strain three grades of kinds, be made up of following raw material according to mass percent: Qinggang ebon leaf of 72% ~ 76%, the wood chip of 24% ~ 28%, the quality sum of above each component is 100%; The water content of described Qinggang ebon leaf is 65% ~ 70%; The water content of described wood chip is 62% ~ 68%; The method comprises the steps:
The first step, gets the raw materials ready
Pluck Qinggang ebon leaf, and dry for subsequent use;
Second step, soaks
Qinggang ebon leaf in the first step is placed in clear water to soak 20 ~ 30 hours;
3rd step, draining
Pull Qinggang ebon leaf that second step is obtained out, be placed in dry place draining, make water content in the ebon leaf of Qinggang be 65% ~ 70%;
4th step, spice
Mix through Qinggang ebon leaf of the 3rd step process according to the ratio that mass ratio is 4:1 with the wood chip of water content 62% ~ 68%, and stir, obtained compound;
5th step, pack
Loaded in strain bag by compound obtained for 4th step, charge weight is 80% ~ 82% of strain bag capacity;
6th step, covers
The remaining space of the strain bag of the 5th step is filled wood chip, and wood chip is covered in the top layer of described compound;
7th step, sterilizing
Strain bag through the 6th step process is put into sterilizing chamber, and the temperature of sterilizing chamber is set to 124 ~ 125 DEG C, and the pressure of sterilizing chamber is set to 0.14 ~ 0.15MPa, carries out sterilization treatment 3 hours;
8th step, cooling
Strain bag through the 7th step process is put into temperature not higher than the indoor of 18 DEG C, cools 3 ~ 5 hours, make the temperature of strain bag not higher than 20 DEG C;
9th step, access secondary mother plants
In an aseptic environment by gastrodia elata sexual propagation Germination Strain original seed access strain bag;
Tenth step, cultivates
Strain bag through the 9th step process being positioned over temperature is cultivate 40 ~ 50 days in the culturing room of 20 ~ 24 DEG C, obtained gastrodia elata sexual propagation Germination Strain three grades of kinds; Gastrodia elata sexual propagation Germination Strain original seed described in 9th step is the one in Mycen osmundicola, dendrobium mixture, Mycena orchidicola or Mycena anoectochila).
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| CN103650918B (en) * | 2013-12-13 | 2016-02-17 | 重庆市福鑫洋食用菌有限公司 | The method of Germination Strain bacterial classification is made of bulk hardwood sawdust |
| CN104541970B (en) * | 2014-12-18 | 2017-10-10 | 河北祁一堂药业有限公司 | The method of northern area outdoor planting rhizoma Gastrodiae |
| CN104663404B (en) * | 2015-03-09 | 2017-05-17 | 昭通市林业科学研究所 | Breeding method for gastrodia elata |
| CN106234065A (en) * | 2016-08-01 | 2016-12-21 | 金寨县郑众中药材种植专业合作社 | A kind of Wild gastrodia cultivation mother culture media |
| CN107135807A (en) * | 2017-05-11 | 2017-09-08 | 江口县厚谊科技开发有限责任公司 | The preparation method that a kind of rhizoma Gastrodiae sprouts bacterium culture medium and its Germination Strain fungus block |
| CN107593266A (en) * | 2017-08-25 | 2018-01-19 | 道真仡佬族苗族自治县联合中药材种植科技服务中心 | Rhizoma Gastrodiae Germination Strain breeding method |
| CN115336503B (en) * | 2022-08-30 | 2024-01-16 | 西南林业大学 | Preparation method of substitute bacteria for gastrodia elata cultivation |
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| CN1038743A (en) * | 1988-06-24 | 1990-01-17 | 中国医学科学院药用植物资源开发研究所 | Mycen osmundicola and gastrodia seed germination culture technique |
| CN1426677A (en) * | 2001-12-18 | 2003-07-02 | 吉林省露水河林业局 | New method of planting rhizoma gastrodiae sexual propagation in shallow ridge furrowed field |
| CN102061263B (en) * | 2010-10-26 | 2012-05-23 | 昭通神农乌天麻良种繁育有限公司 | Gastrodia elata seed Germination bacterium (XL-M0701), and preparation method and application thereof |
| CN102153377B (en) * | 2010-11-04 | 2013-01-23 | 何朝伦 | Culture medium for tall gastrodia tuber germination strains |
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