CN107667856A - A kind of implantation methods for improving polysaccharide content of dendrobium candidum - Google Patents
A kind of implantation methods for improving polysaccharide content of dendrobium candidum Download PDFInfo
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- CN107667856A CN107667856A CN201710888883.8A CN201710888883A CN107667856A CN 107667856 A CN107667856 A CN 107667856A CN 201710888883 A CN201710888883 A CN 201710888883A CN 107667856 A CN107667856 A CN 107667856A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The present invention relates to the planting technology field of the stem of noble dendrobium,Disclose a kind of implantation methods for improving polysaccharide content of dendrobium candidum,To the moisture of culture medium,Ventilation,The situations such as nutrition are regulated and controled,This is the key link for affecting tissue-cultured seedling transplanting survival rate and later stage growing way,Matrix can synthesize nitrogen compound,Amino acid,Physiological activator,A variety of materials for promoting growth and development of plants such as carbohydrate,Also there is the foul smell for reducing organic fertilizer,Strengthen sterilizing power,Particularly suppress the activity of harmful levels of pathogens,Prevent the pernicious corruption of organic matter from suppressing germ and growing,Reach the effect of the benign fermentation of organic fertilizer,The present invention improves the value of dendrobium candidum from outside growing way to internal polyoses content,Plant in obtained 2 years stems of the stem of noble dendrobium,Polyoses content reaches 10.8 11.0% in the thick leaf of iron sheet stone solution,Narrow leaf is 6.8 7.2%,Stem is 18.3 18.8%.
Description
Technical field
The invention belongs to growing dendrobium technical field, and in particular to a kind of plantation side for improving polysaccharide content of dendrobium candidum
Method.
Background technology
The stem of noble dendrobium also known as dendrobium, original producton location are distributed mainly on Tropical Asian and subtropical zone, Australia and Pacific Islands,
Dendrobium is that maximum in orchid family one belongs to, including about 1500 kinds of plants, is distributed mainly on Tropical Asia and Pacific Islands.I
About 63 kinds of Dendrobium Sws of state, are made for 39 kinds of medicinal Dendrobium Sw.The domestic stem of noble dendrobium is distributed in the Qinling Mountains, on the south Huaihe River, from latitude
For degree, most of species are all concentrated between 30 '~25 ° 12 ' of north latitude 15 °, and northwards species gradually decreases, and most arctogaean realm is no more than
34 ° 24 ' of north latitude.From vertically, the height that 100~3000 meters of height above sea level is all distributed.Dendrobium Sw is with tropical Southeast Asia
The monoid that the heart develops towards subtropical zone property weather conditions, Yunnan, Guangxi, Guangdong, Guizhou, the Taiwan in China are domestic platymiscium
Distribution center.Fresh stem of noble dendrobium clearing heat and promoting fluid power is strong, and warm disease and evil is joined the army blood, and when use, dendrobium chrysanthum enriching yin is mended clearly is high fever dysphoria with smothery sensation person
Long, in the pyreticosis later stage, yin deficiency abnormal heat person is suitable.The stem of noble dendrobium can increase substantially SOD in human body (main matter of anti-aging) levels,
To often staying up late, with brain, excessive drinking and smoking, physically weak weak crowd, often drink and be particularly suitable for.
Polysaccharide is the main chemical composition of stone solution platymiscium, the pharmacological action of stone solution polysaccharide be mainly manifested in it is immune,
It is strong etc., there is immunological enhancement and antitumor action.Traditionally stone solution improved seeds " matter weight,
That chews sticks to one's teeth, sweet, and no slag person is excellent ", human body and zoopery confirm, stone solution polysaccharide has enhancing T cell and huge
The effect of phagocytic immunity activity, is a kind of gratifying immunopotentiator of prospect.Therefore, cultivate polyoses content is high, yield is big,
Fast iron sheet stone solution kind is grown, introduces a fine variety and breeds in appropriate area, to strengthen the protection and utilization to plant resources, is met
Growing demand is particularly significant.
The content of the invention
The purpose of the present invention is to be directed to the problem of existing, there is provided a kind of plantation side for improving polysaccharide content of dendrobium candidum
Method, polyoses content reaches 10.8-11.0%, narrow leaf 6.8-7.2%, stem 18.3-18.8% in the thick leaf of iron sheet stone solution.
The present invention is achieved by the following technical solutions：
A kind of implantation methods for improving polysaccharide content of dendrobium candidum, comprise the following steps：
（1）The dendrobium candidum grown on selection remote mountains palisades is maternal as culture, carries out tissue cultures, and obtained differentiation seedling makes
Cultivated with seedling medium, cultivation is operated in shade greenhouse and carried out, and day temperature is in 25-35 DEG C of scope in greenhouse temperature
Interior, night temperatures are in the range of 10-20 DEG C, and relative air humidity is in 75-85% scopes；
（2）The liftoff 60-70 centimetres of height in seedbed is cultivated, apart from 150-160 centimetres of height of ceiling, seedbed 20-25 rice is long, 1.5-1.8 rice
Width, on seedbed, 10-12 centimetres of composite interstitial substance thickness, the composite interstitial substance by fertile soil, bentonite, turfy soil, perlite, peat,
Corncob, melon seed cases, pine bark, the pinecone, waxgourd peel are made, and matrix is using the carbendazim that preceding concentration is 0.04-0.06%
Spray solution, every cubic metre of dosage are 200-230 grams, and ventilation can spread seedbed use after drying 3-5 hours；
（3）By the stem of noble dendrobium seedling cultivation after hardening on seedbed, 3-4 strains are 13-15 cm x 15-18 from line-spacing as one clump
Centimetre, root water is poured after transplanting, is defined by moistening matrix 5-7 cm thicks, after 5-7 days can moisturizing, applying organic manure, nursery 3-
After 4 months, stem of noble dendrobium seedling can be transplanted to crop field together with root matrix, strengthen illumination and water management, reach 1.5-2 lifes
Harvesting.
Further described as to such scheme, the composite interstitial substance is made up of following component in parts by weight：It is rotten
Grow native 80-90 parts, bentonite 75-80 parts, turfy soil 70-75 parts, perlite 50-60 parts, peat 45-50 parts, corncob 35-45
Part, melon seed cases 25-35 parts, pine bark 20-25 parts, pinecone 15-20 parts, waxgourd peel 10-15 parts.
Further described as to such scheme, the corncob, melon seed cases, pine bark, the pinecone, waxgourd peel be by
Used after fermentation process, fermentation process is：EM leavenings are added into above-mentioned raw materials, are 50-80 grams per double centner dosage,
Banked up after mixing, with covered rearing with plastic film fermentation heap, sealed fermenting, after fermentation heap is changed into brown, spreads out and be cooled to room temperature, repeated
Stirring 2-3 times, continue to divide fermentation 10-15 days.
Further described as to such scheme, photosynthetic bacteria group's concentration is 1 × 10 in the EM leavenings9-5×
109CFU/ml scopes, lactobacillus concentration are 1 × 108-5×108CFU/ml scopes, yeast flora concentration are 5 × 108-10×
108CFU/ml scopes, defence line flora concentration are 1 × 107-5×107CFU/ml scopes.
The present invention has advantages below compared with prior art：In order to improve the content of polysaccharide and growth state in dendrobium candidum
Gesture, the invention provides a kind of implantation methods for improving polysaccharide content of dendrobium candidum, to the moisture of culture medium, ventilation, nutrition
Regulated and controled etc. situation, this is the key link for affecting tissue-cultured seedling transplanting survival rate and later stage growing way, and matrix being capable of synthetic nitrogen
A variety of materials for promoting growth and development of plants such as plain compound, amino acid, physiological activator, carbohydrate, also have reduction organic
Foul smell, the enhancing sterilizing power of fertilizer, particularly suppress the activity of harmful levels of pathogens, prevent the pernicious corruption of organic matter from suppressing germ and growing
It is raw, reach the effect of the benign fermentation of organic fertilizer, the present invention improves the profit of dendrobium candidum from outside growing way to internal polyoses content
With value, plant in obtained 2 years stems of the stem of noble dendrobium, polyoses content reaches 10.8-11.0%, narrow leaf 6.8- in the thick leaf of iron sheet stone solution
7.2%, stem 18.3-18.8%.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1
A kind of implantation methods for improving polysaccharide content of dendrobium candidum, comprise the following steps：
（1）The dendrobium candidum grown on selection remote mountains palisades is maternal as culture, carries out tissue cultures, and obtained differentiation seedling makes
Cultivated with seedling medium, cultivation is operated in shade greenhouse and carried out, and day temperature is in 25-35 DEG C of scope in greenhouse temperature
Interior, night temperatures are in the range of 10-20 DEG C, and relative air humidity is in 75-85% scopes；
（2）The liftoff 60 centimetres of height in seedbed are cultivated, apart from 150 centimetres of height of ceiling, seedbed is 20 meters long, 1.5 meters wide, compound on seedbed
10 centimetres of stromal thickness, the composite interstitial substance is by fertile soil, bentonite, turfy soil, perlite, peat, corncob, melon seed cases, pine
Bark, the pinecone, waxgourd peel are made, and matrix is sprayed using the carbendazim solution that preceding concentration is 0.04%, every cubic metre of dosage
For 200 grams, ventilation can spread seedbed use after drying 3 hours；
（3）3 plants of conducts one clump, it is 15 centimetres of 13 cm x from line-spacing by the stem of noble dendrobium seedling cultivation after hardening on seedbed, transplanting
After pour root water, be defined by moistening the cm thick of matrix 5, after 5 days can moisturizing, applying organic manure, can be by stone after nursery 3 months
Dry measure used in former times seedling is transplanted to crop field together with root matrix, strengthens illumination and water management, reaching 1.5-2 lifes can pluck.
Further described as to such scheme, the composite interstitial substance is made up of following component in parts by weight：It is rotten
Grow soil 80,75 parts of bentonite, 70 parts of turfy soil, 50 parts of perlite, 45 parts of peat, 35 parts of corncob, 25 parts of melon seed cases, pine bark
20 parts, 15 parts of the pinecone, 10 parts of waxgourd peel.
Further described as to such scheme, the corncob, melon seed cases, pine bark, the pinecone, waxgourd peel be by
Used after fermentation process, fermentation process is：EM leavenings are added into above-mentioned raw materials, is 50 grams per double centner dosage, mixes
After bank up, with covered rearing with plastic film fermentation heap, sealed fermenting, after fermentation heap is changed into brown, spreads out and be cooled to room temperature, repeat stir
2- times, continue to divide fermentation 10 days.
Further described as to such scheme, photosynthetic bacteria group's concentration is 1 × 10 in the EM leavenings9-5×
109CFU/ml scopes, lactobacillus concentration are 1 × 108-5×108CFU/ml scopes, yeast flora concentration are 5 × 108-10×
108CFU/ml scopes, defence line flora concentration are 1 × 107-5×107CFU/ml scopes.
Embodiment 2
A kind of implantation methods for improving polysaccharide content of dendrobium candidum, comprise the following steps：
（1）The dendrobium candidum grown on selection remote mountains palisades is maternal as culture, carries out tissue cultures, and obtained differentiation seedling makes
Cultivated with seedling medium, cultivation is operated in shade greenhouse and carried out, and day temperature is in 25-35 DEG C of scope in greenhouse temperature
Interior, night temperatures are in the range of 10-20 DEG C, and relative air humidity is in 75-85% scopes；
（2）The liftoff 65 centimetres of height in seedbed are cultivated, apart from 155 centimetres of height of ceiling, seedbed is 22 meters long, 1.6 meters wide, compound on seedbed
11 centimetres of stromal thickness, the composite interstitial substance is by fertile soil, bentonite, turfy soil, perlite, peat, corncob, melon seed cases, pine
Bark, the pinecone, waxgourd peel are made, and matrix is sprayed using the carbendazim solution that preceding concentration is 0.05%, every cubic metre of dosage
For 210 grams, ventilation can spread seedbed use after drying 4 hours；
（3）3 plants of conducts one clump, it is 16 centimetres of 14 cm x from line-spacing by the stem of noble dendrobium seedling cultivation after hardening on seedbed, transplanting
After pour root water, be defined by moistening the cm thick of matrix 6, after 6 days can moisturizing, applying organic manure, can will after nursery 3.5 months
Stem of noble dendrobium seedling is transplanted to crop field together with root matrix, strengthens illumination and water management, reaching 1.5-2 lifes can pluck.
Further described as to such scheme, the composite interstitial substance is made up of following component in parts by weight：It is rotten
Grow 85 parts of soil, 78 parts of bentonite, 73 parts of turfy soil, 55 parts of perlite, 48 parts of peat, 40 parts of corncob, 30 parts of melon seed cases, pine tree
22 parts of skin, 18 parts of the pinecone, 12 parts of waxgourd peel.
Further described as to such scheme, the corncob, melon seed cases, pine bark, the pinecone, waxgourd peel be by
Used after fermentation process, fermentation process is：EM leavenings are added into above-mentioned raw materials, is 60 grams per double centner dosage, mixes
After bank up, with covered rearing with plastic film fermentation heap, sealed fermenting, after fermentation heap is changed into brown, spreads out and be cooled to room temperature, repeat stir
2 times, continue to divide fermentation 12 days.
Further described as to such scheme, photosynthetic bacteria group's concentration is 1 × 10 in the EM leavenings9-5×
109CFU/ml scopes, lactobacillus concentration are 1 × 108-5×108CFU/ml scopes, yeast flora concentration are 5 × 108-10×
108CFU/ml scopes, defence line flora concentration are 1 × 107-5×107CFU/ml scopes.
Embodiment 3
A kind of implantation methods for improving polysaccharide content of dendrobium candidum, comprise the following steps：
（1）The dendrobium candidum grown on selection remote mountains palisades is maternal as culture, carries out tissue cultures, and obtained differentiation seedling makes
Cultivated with seedling medium, cultivation is operated in shade greenhouse and carried out, and day temperature is in 25-35 DEG C of scope in greenhouse temperature
Interior, night temperatures are in the range of 10-20 DEG C, and relative air humidity is in 75-85% scopes；
（2）The liftoff 70 centimetres of height in seedbed are cultivated, apart from 160 centimetres of height of ceiling, seedbed is 25 meters long, 1.8 meters wide, compound on seedbed
12 centimetres of stromal thickness, the composite interstitial substance is by fertile soil, bentonite, turfy soil, perlite, peat, corncob, melon seed cases, pine
Bark, the pinecone, waxgourd peel are made, and matrix is sprayed using the carbendazim solution that preceding concentration is 0.06%, every cubic metre of dosage
For 230 grams, ventilation can spread seedbed use after drying 5 hours；
（3）4 plants of conducts one clump, it is 18 centimetres of 15 cm x from line-spacing by the stem of noble dendrobium seedling cultivation after hardening on seedbed, transplanting
After pour root water, be defined by moistening the cm thick of matrix 7, after 7 days can moisturizing, applying organic manure, can be by stone after nursery 4 months
Dry measure used in former times seedling is transplanted to crop field together with root matrix, strengthens illumination and water management, reaching 1.5-2 lifes can pluck.
Further described as to such scheme, the composite interstitial substance is made up of following component in parts by weight：It is rotten
Grow 90 parts of soil, 80 parts of bentonite, 75 parts of turfy soil, 60 parts of perlite, 50 parts of peat, 45 parts of corncob, 35 parts of melon seed cases, pine tree
25 parts of skin, 20 parts of the pinecone, 15 parts of waxgourd peel.
Further described as to such scheme, the corncob, melon seed cases, pine bark, the pinecone, waxgourd peel be by
Used after fermentation process, fermentation process is：EM leavenings are added into above-mentioned raw materials, is 80 grams per double centner dosage, mixes
After bank up, with covered rearing with plastic film fermentation heap, sealed fermenting, after fermentation heap is changed into brown, spreads out and be cooled to room temperature, repeat stir
3 times, continue to divide fermentation 15 days.
Further described as to such scheme, photosynthetic bacteria group's concentration is 1 × 10 in the EM leavenings9-5×
109CFU/ml scopes, lactobacillus concentration are 1 × 108-5×108CFU/ml scopes, yeast flora concentration are 5 × 108-10×
108CFU/ml scopes, defence line flora concentration are 1 × 107-5×107CFU/ml scopes.
Comparative example 1
With differing only in for embodiment 1, step is omitted（1）The step of middle stem of noble dendrobium seedling selection, use common stem of noble dendrobium seedling
Instead of remaining is consistent.
Comparative example 2
With differing only in for embodiment 2, by step（2）The middle seedling medium used changes common seedling medium into, remaining guarantor
Hold consistent.
Comparative example 3
With differing only in for embodiment 3, nursery duration foreshortens to 2 months, and remaining is consistent.
Contrast experiment
Respectively using embodiment 1-3 and comparative example 1-3 method processing plantation stem of noble dendrobium seedling, while with existing growing dendrobium side
Method is control, and each group is tested and carried out simultaneously in areal, records the speed of growth and yield, plucks 2 years raw each group iron sheets
The stem of noble dendrobium, various pieces are divided to carry out sample preparation, every group is produced 10 samples, carries out measurement of the polysaccharide content, assembly average, as a result
It is as shown in the table：
| Project | Polyoses content in thick leaf（%） | Polyoses content in narrow leaf（%） | Polyoses content in stem（%） | Output increased（%） |
| Embodiment 1 | 10.8 | 6.8 | 18.3 | 23.2 |
| Embodiment 2 | 11.0 | 7.2 | 18.8 | 23.6 |
| Embodiment 3 | 10.9 | 7.0 | 18.5 | 23.4 |
| Comparative example 1 | 7.6 | 4.6 | 13.7 | 16.5 |
| Comparative example 2 | 6.8 | 4.2 | 12.4 | 11.0 |
| Comparative example 3 | 7.3 | 4.5 | 12.9 | 16.2 |
| Control group | 6.5 | 3.8 | 11.7 | Control |
As can be seen here：The implantation methods provided by the invention for improving polysaccharide content of dendrobium candidum, from outside growing way to internal polysaccharide
Content improves the value of dendrobium candidum, plants in the obtained stem of noble dendrobium 2 years stems, polyoses content reaches in the thick leaf of iron sheet stone solution
10.8-11.0%, narrow leaf 6.8-7.2%, stem 18.3-18.8%.
Claims (4)
1. a kind of implantation methods for improving polysaccharide content of dendrobium candidum, it is characterised in that comprise the following steps：
（1）The dendrobium candidum grown on selection remote mountains palisades is maternal as culture, carries out tissue cultures, and obtained differentiation seedling makes
Cultivated with seedling medium, cultivation is operated in shade greenhouse and carried out, and day temperature is in 25-35 DEG C of scope in greenhouse temperature
Interior, night temperatures are in the range of 10-20 DEG C, and relative air humidity is in 75-85% scopes；
（2）The liftoff 60-70 centimetres of height in seedbed is cultivated, apart from 150-160 centimetres of height of ceiling, seedbed 20-25 rice is long, 1.5-1.8 rice
Width, on seedbed, 10-12 centimetres of composite interstitial substance thickness, the composite interstitial substance by fertile soil, bentonite, turfy soil, perlite, peat,
Corncob, melon seed cases, pine bark, the pinecone, waxgourd peel are made, and matrix is using the carbendazim that preceding concentration is 0.04-0.06%
Spray solution, every cubic metre of dosage are 200-230 grams, and ventilation can spread seedbed use after drying 3-5 hours；
（3）By the stem of noble dendrobium seedling cultivation after hardening on seedbed, 3-4 strains are 13-15 cm x 15-18 from line-spacing as one clump
Centimetre, root water is poured after transplanting, is defined by moistening matrix 5-7 cm thicks, after 5-7 days can moisturizing, applying organic manure, nursery 3-
After 4 months, stem of noble dendrobium seedling can be transplanted to crop field together with root matrix, strengthen illumination and water management, reach 1.5-2 lifes
Harvesting.
A kind of 2. implantation methods for improving polysaccharide content of dendrobium candidum as claimed in claim 1, it is characterised in that the composite base
Matter is made up of following component in parts by weight：Fertile soil 80-90 parts, bentonite 75-80 parts, turfy soil 70-75 parts, perlite
50-60 parts, peat 45-50 parts, corncob 35-45 parts, melon seed cases 25-35 parts, pine bark 20-25 parts, pinecone 15-20 parts, winter
Melon skin 10-15 parts.
A kind of 3. implantation methods for improving polysaccharide content of dendrobium candidum as claimed in claim 1, it is characterised in that the corn
Core, melon seed cases, pine bark, the pinecone, waxgourd peel use after fermentation process, and fermentation process is：Add into above-mentioned raw materials
Add EM leavenings, be 50-80 grams per double centner dosage, banked up after mixing, with covered rearing with plastic film fermentation heap, sealed fermenting, hair
After ferment heap is changed into brown, spreads out and be cooled to room temperature, repeat stirring 2-3 times, continue to divide fermentation 10-15 days.
A kind of 4. implantation methods for improving polysaccharide content of dendrobium candidum as claimed in claim 3, it is characterised in that the EM fermentations
Photosynthetic bacteria group concentration is 1 × 10 in agent9-5×109CFU/ml scopes, lactobacillus concentration are 1 × 108-5×108CFU/ml
Scope, yeast flora concentration are 5 × 108-10×108CFU/ml scopes, defence line flora concentration are 1 × 107-5×107CFU/ml
Scope.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108812194A (en) * | 2018-06-14 | 2018-11-16 | 安徽省霍山县农伯乐开发有限公司 | A kind of cultivation matrix promoting dendrobium nutrient value and yield |
| CN109429992A (en) * | 2018-10-26 | 2019-03-08 | 含山县贝亲生物科技有限公司 | A kind of preparation method for the Nutrition Soil improving cactus anti-oxidation active substance content |
-
2017
- 2017-09-27 CN CN201710888883.8A patent/CN107667856A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 安彦峰: "不同栽培基质对铁皮石斛有效成分含量的影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108812194A (en) * | 2018-06-14 | 2018-11-16 | 安徽省霍山县农伯乐开发有限公司 | A kind of cultivation matrix promoting dendrobium nutrient value and yield |
| CN109429992A (en) * | 2018-10-26 | 2019-03-08 | 含山县贝亲生物科技有限公司 | A kind of preparation method for the Nutrition Soil improving cactus anti-oxidation active substance content |
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