CN104663404B - Breeding method for gastrodia elata - Google Patents
Breeding method for gastrodia elata Download PDFInfo
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- CN104663404B CN104663404B CN201510101686.8A CN201510101686A CN104663404B CN 104663404 B CN104663404 B CN 104663404B CN 201510101686 A CN201510101686 A CN 201510101686A CN 104663404 B CN104663404 B CN 104663404B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a breeding method for gastrodia elata. After cunninghamia lanceolata sawdust and shale-free sand are uniformly stirred, the stirred substance is sprayed with water to be wet and the wet substance replaces sandy soil as a substrate, so that the weight of each breeding bag filled with gastrodia elata seeds is reduced from 15-20kg to 7-8kg, the labor intensity is reduced, and the cost input is reduced; when the breeding bags are placed under shading woods, no covering is needed, the management is facilitated, and the yield is stable; the yield of the gastrodia elata is increased from about 0.2kg of each bag on average in the past to over 0.8kg of each bag, and planting benefits are remarkably improved.
Description
Technical field
The invention belongs to Rhizoma Gastrodiae breeding technical field, and in particular to a kind of germination rate is high, yield is high, adaptable Rhizoma Gastrodiae
Breeding method.
Background technology
Rhizoma Gastrodiae, is orchid family Gastrodia herbaceos perennial also known as rhizoma gastrodiae, and root stock is plump, and without greenery, capsule falls ovate
Ellipse, often with tuber or seminal propagation, its rhizome be used as medicine to treat have a dizzy spell, the disease such as numb limbs and tense tendons, infantile convulsion, be
Famous and precious Chinese medicine.
Due to the medical value of Rhizoma Gastrodiae it is high, market demand greatly, therefore the Rhizoma Gastrodiae state that is constantly in that supply falls short of demand.From
Nineteen seventies, just there are many researchs to Rhizoma Gastrodiae Propogation and culture technology, yet with Rhizoma Gastrodiae Cultivating techniques
It is immature, hinder the development of Rhizoma Gastrodiae planting industry.
The growth of Rhizoma Gastrodiae is for giving nutrition by armillaria mellea, it is not necessary to nutrient is absorbed from soil, and soil only plays substrate and consolidates
Fixed effect, therefore gradually develop packed Rhizoma Gastrodiae implantation methods.At present packed Rhizoma Gastrodiae breeding method is generally made using sandy soil
Cultivation bag bottom is laid on for substrate, one layer of seeds of Gastrodia elata sprouted is placed above substrate, place bacterium material and armillaria mellea, so
Put one layer of seeds of Gastrodia elata sprouted again afterwards, cover the substrate after 10cm, bag will be cultivated and tied, be placed into where selecting, then
Cultivating mulching soil outside bag;So Rhizoma Gastrodiae grows naturally in bag is cultivated, and to autumn then, Rhizoma Gastrodiae zero generation kind grows up to, and arrives
Autumn next year Rhizoma Gastrodiae generation kind grows up to, and will cultivate bag and tears, and substrate is poured out, and takes out Rhizoma Gastrodiae hoary hair fiber crops and plants, breeding ends.So
And, using needing cultivating bag outer covering soil during above-mentioned packed breeding method, because blinding is thicker, Rhizoma Gastrodiae sprouts the soil of layer
Temperature be cannot get up, and Rhizoma Gastrodiae is perishable, cause that Rhizoma Gastrodiae germination rate is low, yield is not high, while cultivating the work of Rhizoma Gastrodiae and collecting tuber of elevated gastrodia
It is relatively costly.
The content of the invention
It is an object of the invention to provide a kind of germination rate is high, yield is high, adaptable Rhizoma Gastrodiae breeding method.
The object of the present invention is achieved like this, including seed selection, seed are sprouted and cultivated and hoary hair's fiber crops cultivation, concrete steps
For:
A, seed selection:Good face shaping, no disease and pests harm, not damaged, nothing is selected to rot speckle, the fiber crops of the arrow without deformity as kind
The seeds of Gastrodia elata of maturation is cultivated in fiber crops, Jing artificial pollinations after plantation;Select the Germination Strain that strain growth is good, germination rate is high, Jing trainings
Support and select strain excellent;It is armillaria mellea production kind from the isolated ripe three-level armillaria mellea of armillaria mellea sporophore;
B, seed are sprouted and are cultivated:The bag of Germination Strain one of 0.5kg is taken, to be torn and mix Rhizoma Gastrodiae fruit 5 ~ 8 after dissipating, modeling is loaded after mixing
Pocket, is put in culture room and cultivates more than 72h, and to Germination Strain restoration ecosystem in bulk, seed is sprouted cultivation and completed;
C, hoary hair fiber crops are cultivated:Substrate of the bag bottom laying depth for 2 ~ 3cm is being cultivated, uniform one layer of the placement in substrate is sprouted
The seeds of Gastrodia elata sent out;From the bacterium material of long 15cm, three row's fish scale mouths are cut on bacterium material, fish scale mouth is spaced 3 ~ 5cm, will cut fish
The bacterium material of squama mouth is fixed in cultivation bag, and armillaria mellea production kind is uniformly placed at each wound of bacterium material, covers thick layer
The substrate for 3 ~ 5cm is spent, it is then again uniform in substrate to place one layer of seeds of Gastrodia elata sprouted;The bacterium of fish scale mouth will be cut again
Material is fixed in cultivation bag, and armillaria mellea production kind is uniformly placed at each wound of bacterium material, and covering a layer thickness is 3 ~ 5cm
Substrate, one layer of seeds of Gastrodia elata sprouted then uniformly is placed in substrate again, then cover base of a layer thickness for 3 ~ 5cm
Matter;Bag will be cultivated to tie, shelter from heat or light sylvan life or unvegetated slope is positioned over.
Water spray is moist after the substrate is mixed thoroughly for Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale, wherein Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale
Volume ratio be 2:1.
Rhizoma Gastrodiae is grown under light protected environment in whole growth course, all, suitable humiture is only needed, while Rhizoma Gastrodiae
Growth is not required to absorb nutrient in soil, and soil is only used as fixing the substrate of bacterium material, and the present invention is using Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and without shale
Sand mix thoroughly after the moist sandy soil of replacing of water spray as substrate so that install the weight of cultivation bag of seeds of Gastrodia elata from original 15
~ 20kg is reduced to 7 ~ 8kg, reduces labor intensity, and reduces cost input;Cultivation bag is positioned over when sheltering from heat or light sylvan life, is not required to cover, just
In management, stable yield;Output of Gastrodia elata increases to every bag of more than 0.8kg from per bag of average 0.2kg of past highest or so, plantation
Benefit is significantly improved.
Specific embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is any limitation as never in any form,
Based on present invention teach that any change for being made or improvement, belong to protection scope of the present invention.
The Rhizoma Gastrodiae breeding method that the present invention is provided includes that seed selection, seed sprouting are cultivated and hoary hair fiber crops are cultivated, and concretely comprises the following steps:
A, seed selection:Good face shaping, no disease and pests harm, not damaged, nothing is selected to rot speckle, the fiber crops of the arrow without deformity as kind
The seeds of Gastrodia elata of maturation is cultivated in fiber crops, Jing artificial pollinations after plantation;Select the Germination Strain that strain growth is good, germination rate is high, Jing trainings
Support and select strain excellent;It is armillaria mellea production kind from the isolated ripe three-level armillaria mellea of armillaria mellea sporophore;
B, seed are sprouted and are cultivated:The bag of Germination Strain one of 0.5kg is taken, to be torn and mix Rhizoma Gastrodiae fruit 5 ~ 8 after dissipating, modeling is loaded after mixing
Pocket, is put in culture room and cultivates more than 72h, and to Germination Strain restoration ecosystem in bulk, seed is sprouted cultivation and completed;
C, hoary hair fiber crops are cultivated:Substrate of the bag bottom laying depth for 2 ~ 3cm is being cultivated, uniform one layer of the placement in substrate is sprouted
The seeds of Gastrodia elata sent out;From the bacterium material of long 15cm, three row's fish scale mouths are cut on bacterium material, fish scale mouth is spaced 3 ~ 5cm, will cut fish
The bacterium material of squama mouth is fixed in cultivation bag, and armillaria mellea production kind is uniformly placed at each wound of bacterium material, covers thick layer
The substrate for 3 ~ 5cm is spent, it is then again uniform in substrate to place one layer of seeds of Gastrodia elata sprouted;The bacterium of fish scale mouth will be cut again
Material is fixed in cultivation bag, and armillaria mellea production kind is uniformly placed at each wound of bacterium material, and covering a layer thickness is 3 ~ 5cm
Substrate, one layer of seeds of Gastrodia elata sprouted then uniformly is placed in substrate again, then cover base of a layer thickness for 3 ~ 5cm
Matter;Bag will be cultivated to tie, sylvan life of sheltering from heat or light is positioned over.
The Germination Strain is from Mycen osmundicola or dendrobium mixture.
Water spray is moist after the substrate is mixed thoroughly for Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale, wherein Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale
Volume ratio be 2:1.
The cultivation bag can also be positioned over unvegetated slope, when unvegetated slope is positioned over, need to cultivate
One layer of grass is covered on bag.
The air humidity in selected shelter from heat or light sylvan life or unvegetated hillside fields is 60% ~ 85%.
Embodiment 1
In Rhizoma Gastrodiae collection period, select the arrow fiber crops of local original seed as planting fiber crops, it is desirable to its ontogeny completely, healthy and strong, terminal bud
Full, not damaged, single weight are in more than 100g;The kind fiber crops chosen are planted in moisture in the soil or flowerpot, kind of a numb Post flowering is treated, it is right
It carries out artificial pollination, as far as possible using xenogamy, after pollination 20 days or so, mature fruit is harvested, and 6 are taken after harvesting
The Mycen osmundicola uniform mixing of fruit and a bag 0.5kg, loads plastic bag, is put in culture room and cultivates 72h, cultivates the temperature in room
At 18 DEG C ~ 20 DEG C, humidity 70% ~ 80%, lucifuge keeps ventilation for control;Cultivating substrate of the bag bottom laying depth for 2cm, institute
Water spray is moist after stating substrate and mixing thoroughly for Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale, and wherein Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust is with the volume ratio of the sand without shale
2:1;One layer of band kind Mycen osmundicola broken up uniformly is placed in substrate;From the bacterium material of long 15cm, three row fishes are cut on bacterium material
Squama mouth, fish scale mouth is spaced 3 ~ 5cm, and the bacterium material for having cut fish scale mouth is fixed in cultivation bag, uniform at each wound of bacterium material
The production kind of placement armillaria mellea, cover substrate of a layer thickness for 3cm, one layer of band broken up then uniformly is placed in substrate again
Plant Mycen osmundicola;The bacterium material for having cut fish scale mouth is fixed in cultivation bag again, uniformly places close at each wound of bacterium material
The production kind of ring bacterium, covers substrate of a layer thickness for 3cm, then and in substrate uniformly places one layer of band kind osmund broken up little
Mushroom, then place the substrate of one layer of 3cm;Then bag will be cultivated to tie, sylvan life of sheltering from heat or light is positioned over, the air humidity of sylvan life of sheltering from heat or light is
60% ~ 85%, seeds of Gastrodia elata self-growth in bag is cultivated can open the hoary hair fiber crops of bag harvesting growth and maturity, i.e., to autumn next year
Obtain Rhizoma Gastrodiae generation kind and part arrow fiber crops.In cultivating practice, applicant in 2013 presses in the little fascine dam in Yunnan Province Zhaotong City Yiliang County
Existing breeding method plants 2218 bags of powder fiber crops, and in December, 2014 harvesting obtains 472.5kg hoary hair fiber crops and 18.5kg arrows fiber crops, and per bag total
Average product is 0.22kg, and hoary hair's fiber crops average product is 0.21kg;The breeding method provided using the present invention is while in same place
256 bags of powder fiber crops of plantation, in December, 2014 harvesting obtains 206.5kg hoary hair fiber crops and 56kg arrows fiber crops, and every bag of grand mean yield is
1.03kg, hoary hair fiber crops average product 0.81kg, yield effectively improves.
Embodiment 2
In Rhizoma Gastrodiae collection period, select the arrow fiber crops of local original seed as planting fiber crops, it is desirable to its ontogeny completely, healthy and strong, terminal bud
Full, not damaged, single weight are in more than 100g;The kind fiber crops chosen are planted in moisture in the soil or flowerpot, kind of a numb Post flowering is treated, it is right
It carries out artificial pollination, as far as possible using xenogamy, after pollination 20 days or so, mature fruit is harvested, and 6 are taken after harvesting
The dendrobium mixture uniform mixing of fruit and a bag 0.5kg, loads plastic bag, is put in culture room and cultivates 72h, cultivates the temperature in room
At 18 DEG C ~ 20 DEG C, humidity 70% ~ 80%, lucifuge keeps ventilation for control;Cultivating substrate of the bag bottom laying depth for 3cm, institute
Water spray is moist after stating substrate and mixing thoroughly for Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale, and wherein Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust is with the volume ratio of the sand without shale
2:1;One layer of band kind Mycen osmundicola broken up uniformly is placed in substrate;From the bacterium material of long 15cm, three row fishes are cut on bacterium material
Squama mouth, fish scale mouth is spaced 3 ~ 5cm, and the bacterium material for having cut fish scale mouth is fixed in cultivation bag, uniform at each wound of bacterium material
The production kind of placement armillaria mellea, cover the thick substrate of one layer of 5cm, it is then again uniform in substrate that to place one layer of band kind broken up purple
The little mushroom of beanstalk;The bacterium material for having cut fish scale mouth is fixed in cultivation bag again, at each wound of bacterium material armillaria mellea is uniformly placed
Production kind, covers the thick substrate of one layer of 5cm, then and in substrate uniformly places one layer of band kind Mycen osmundicola broken up, then covers
One layer of 5cm of lid thick substrate;Then bag will be cultivated to tie, sylvan life of sheltering from heat or light is positioned over, the air humidity of sylvan life of sheltering from heat or light is 60% ~
85%, seeds of Gastrodia elata self-growth in bag is cultivated can open the hoary hair fiber crops of bag harvesting growth and maturity to autumn next year, that is, obtain
Rhizoma Gastrodiae generation kind and part arrow fiber crops.In cultivating practice, applicant in 2013 is in the little fascine dam in Yunnan Province Zhaotong City Yiliang County by existing
Breeding method plants 518 bags of powder fiber crops, and in December, 2014 harvesting obtains 104.96kg hoary hair fiber crops and 4.5kg arrows fiber crops, every bag of grand mean
Yield is 0.21kg, and hoary hair's fiber crops average product is 0.2kg;The breeding method provided using the present invention is planted in same place simultaneously
156 bags of powder fiber crops, in December, 2014 harvesting obtains 129.48kg hoary hair fiber crops and 40.56kg arrows fiber crops, and every bag of grand mean yield is
1.09kg, hoary hair fiber crops average product 0.83kg, yield effectively improves.
Embodiment 3
In Rhizoma Gastrodiae collection period, select the arrow fiber crops of local original seed as planting fiber crops, it is desirable to its ontogeny completely, healthy and strong, terminal bud
Full, not damaged, single weight are in more than 100g;The kind fiber crops chosen are planted in moisture in the soil or flowerpot, kind of a numb Post flowering is treated, it is right
It carries out artificial pollination, as far as possible using xenogamy, after pollination 20 days or so, mature fruit is harvested, and 6 are taken after harvesting
The dendrobium mixture uniform mixing of fruit and a bag 0.5kg, loads plastic bag, is put in culture room and cultivates 72h, cultivates the temperature in room
At 18 DEG C ~ 20 DEG C, humidity 70% ~ 80%, lucifuge keeps ventilation for control;In the substrate that cultivation bag bottom laying depth is 2.5cm,
Water spray is moist after the substrate is mixed thoroughly for Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale, wherein the volume ratio of Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale
For 2:1;One layer of band kind Mycen osmundicola broken up uniformly is placed in substrate;From the bacterium material of long 15cm, three rows are cut on bacterium material
Fish scale mouth, fish scale mouth is spaced 3 ~ 5cm, and the bacterium material for having cut fish scale mouth is fixed in cultivation bag, equal at each wound of bacterium material
Even placement armillaria mellea production kind, covers the thick substrate of one layer of 4cm, then and in substrate uniformly places one layer of band kind broken up
Mycen osmundicola;The bacterium material for having cut fish scale mouth is fixed in cultivation bag again, at each wound of bacterium material close ring is uniformly placed
Bacterium production kind, covers the thick substrate of one layer of 4cm, then and in substrate uniformly places one layer of band kind Mycen osmundicola broken up, then
Cover the thick substrate of one layer of 4cm;Then bag will be cultivated to tie, unvegetated slope is positioned over, is then covered on bag is cultivated
One layer of grass, the air humidity in unvegetated hillside fields is 60% ~ 85%, seeds of Gastrodia elata self-growth in bag is cultivated, to autumn next year
The hoary hair fiber crops of bag harvesting growth and maturity can be opened, that is, obtains Rhizoma Gastrodiae generation kind and part arrow fiber crops.In cultivating practice, apply within 2013
People plants 620 bags of powder fiber crops in the little fascine dam in Yunnan Province Zhaotong City Yiliang County by existing breeding method, and in December, 2014 harvesting is obtained
115kg hoary hair fiber crops and 5kg arrows fiber crops, every bag of grand mean yield is 0.2kg, and hoary hair's fiber crops average product is 0.185kg;Using the present invention
The breeding method of offer plants 106 bags of powder fiber crops in same place simultaneously, in December, 2014 harvesting obtain 84.8kg hoary hair fiber crops and
22.26kg arrows fiber crops, every bag of grand mean yield is 1.01kg, and hoary hair fiber crops average product 0.8kg, yield effectively improves.
Rhizoma Gastrodiae is grown under light protected environment in whole growth course, all, suitable humiture is only needed, while Rhizoma Gastrodiae
Growth is not required to absorb nutrient in soil, and soil is only used as fixing the substrate of bacterium material, and the present invention is using Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and without shale
Sand mix thoroughly after the moist sandy soil of replacing of water spray as substrate so that install the weight of cultivation bag of seeds of Gastrodia elata from original 15
~ 20kg is reduced to 7 ~ 8kg, reduces labor intensity, and reduces cost input;Cultivation bag is positioned over when sheltering from heat or light sylvan life, is not required to cover, just
In management, stable yield;Output of Gastrodia elata increases to every bag of more than 0.8kg from per bag of average 0.2kg of past highest or so, plantation
Benefit is significantly improved.
Claims (2)
1. a kind of Rhizoma Gastrodiae breeding method, it is characterised in that sprout including seed selection, seed and cultivate and hoary hair fiber crops are cultivated, concrete steps
For:
A, seed selection:Select good face shaping, no disease and pests harm, not damaged, nothing to rot speckle, the fiber crops of the arrow without deformity as kind fiber crops, plant
The seeds of Gastrodia elata of maturation is cultivated in Jing artificial pollinations after plant;The Germination Strain that strain growth is good, germination rate is high, Jing cultures is selected to select
Strain excellent;It is armillaria mellea production kind from the isolated ripe three-level armillaria mellea of armillaria mellea sporophore;
B, seed are sprouted and are cultivated:The bag of Germination Strain one of 0.5kg is taken, to be torn and mix Rhizoma Gastrodiae fruit 5 ~ 8 after dissipating, plastics are loaded after mixing
Bag, is put in culture room and cultivates more than 72h, and to Germination Strain restoration ecosystem in bulk, seed is sprouted cultivation and completed;
C, hoary hair fiber crops are cultivated:Substrate of the bag bottom laying depth for 2 ~ 3cm is being cultivated, uniform one layer of the placement in substrate is sprouted
Seeds of Gastrodia elata;From the bacterium material of long 15cm, three row's fish scale mouths are cut on bacterium material, fish scale mouth is spaced 3 ~ 5cm, will cut fish scale mouth
Bacterium material be fixed on cultivation bag in, uniformly place at each wound of bacterium material armillaria mellea production kind, cover a layer thickness be 3
The substrate of ~ 5cm is then again uniform in substrate to place one layer of seeds of Gastrodia elata sprouted;The bacterium material for having cut fish scale mouth is consolidated again
Due to cultivating in bag, armillaria mellea production kind is uniformly placed at each wound of bacterium material, cover base of a layer thickness for 3 ~ 5cm
Matter, it is then again uniform in substrate to place one layer of seeds of Gastrodia elata sprouted, then cover the substrate that a layer thickness is 3 ~ 5cm;Will
Cultivate bag to tie, be positioned over sylvan life of sheltering from heat or light, be not required to cover or be positioned over unvegetated slope, cover one layer on cultivation bag
Grass, air humidity is 60% ~ 85%;The substrate is that water spray is moist after Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust and the sand without shale are mixed thoroughly, Lignum seu Ramulus Cunninghamiae Lanceolatae sawdust with
The volume ratio of the sand without shale is 2:1.
2. Rhizoma Gastrodiae breeding method according to claim 1, it is characterised in that the Germination Strain is from Mycen osmundicola or Herba Dendrobii
Little mushroom.
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CN105660342A (en) * | 2016-04-22 | 2016-06-15 | 贵州省闲草堂医药科技有限公司 | Indoor soilless Gastrodia elata f.glauca cultivation method |
CN105961008A (en) * | 2016-06-03 | 2016-09-28 | 范金明 | Planting method for Gastrodia elata f. glauca and G. elata. BI. f elata hybrid gastrodia elata |
CN107771667B (en) * | 2016-08-31 | 2021-06-08 | 南漳致远农业科技有限公司 | Method for improving seed germination rate in gastrodia elata sexual propagation breeding process |
CN108811968A (en) * | 2018-06-28 | 2018-11-16 | 贵州省德均农特产品开发有限公司 | The box planting unit of Rhizoma Gastrodiae and its method for planting rhizoma gastrodiae |
CN109258362A (en) * | 2018-08-30 | 2019-01-25 | 郝哲 | A kind of sexual breeding method of sand ground Rhizoma Gastrodiae |
CN109275532A (en) * | 2018-10-31 | 2019-01-29 | 黎平青江农业开发有限公司 | A kind of environmentally friendly cultural method of high yield Rhizoma Gastrodiae |
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CN1135829A (en) * | 1995-05-17 | 1996-11-20 | 吴怀崇 | Technology and special device for culturing tall gastrodia in bags |
CN101180933A (en) * | 2007-12-12 | 2008-05-21 | 大方县九龙天麻开发有限公司 | Imitating wildness cultural technique for gastrodia elata |
CN101185396B (en) * | 2007-12-12 | 2010-12-15 | 大方县九龙天麻开发有限公司 | Gastrodia tuber imitative wild sexual reproduction breeding technique |
CN101695247B (en) * | 2009-10-28 | 2011-06-01 | 中国科学院西双版纳热带植物园 | Method for comprehensively preventing and controlling plant diseases and insect pests in artificial gastrodia cultivation under wild-simulated condition |
CN102783324A (en) * | 2011-05-17 | 2012-11-21 | 吉林农业大学 | Method for processing rhizoma gastrodiae seeds by germination bacteria |
CN103430767B (en) * | 2013-08-02 | 2015-08-19 | 洋县新成魔芋菌业科技有限公司 | The medium of gastrodia elata sexual propagation Germination Strain three grades of kinds and breeding method |
CN103548562B (en) * | 2013-10-23 | 2015-08-05 | 关岭百龙汇生物科技有限公司 | A kind of high-yield cultivating method of gastrodia elata sexual propagation |
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