CN112514710A - Arbuscular mycorrhizal fungi continuous type propagation incubator - Google Patents
Arbuscular mycorrhizal fungi continuous type propagation incubator Download PDFInfo
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Abstract
A continuous type propagation culture box for arbuscular mycorrhizal fungi comprises a plant culture box and a hypha propagation culture box arranged on one side of the plant culture box, wherein the plant culture box and the hypha propagation culture box are communicated through a plurality of hypha propagation diffusion channels; the bottom of the hypha propagation expanding box is a V-shaped structure which is surrounded by two inclined bottom plates and is provided with a substrate discharge port, the middle part of the V-shaped structure is horizontally provided with a partition plate which can be drawn out, and the inside of the hypha propagation expanding box is divided into a ventilating cavity communicated with the substrate discharge port and a hypha propagation expanding area above the partition plate by the partition plate. The plant culture box is communicated with the hypha propagation box through the hypha propagation diffusion channel, so that arbuscular mycorrhizal fungi and fibrous roots of plants enter the hypha propagation diffusion channel and enter a matrix in the hypha propagation box, the extractable partition plate can conveniently lead out the matrix containing the hypha and spores of the arbuscular mycorrhizal fungi to prepare the arbuscular mycorrhizal fungi microbial inoculum, and a new matrix is added into the hypha propagation box, so that continuous propagation is realized.
Description
Technical Field
The invention relates to the propagation of arbuscular mycorrhizal fungi in the field of microorganisms, in particular to a continuous propagation culture box for arbuscular mycorrhizal fungi.
Background
Mycorrhiza is the result of co-evolution of the plant with mycorrhizal fungi during long-term survival. Its existence is not only beneficial to improving the ability of plant to resist adverse environment and promoting plant growth, but also beneficial to the survival of mycorrhizal fungi. This relationship sometimes develops to the extent that both parties are difficult to distinguish, plants lack mycorrhiza and cannot survive, and mycorrhiza lacks essential plant root system symbiosis, cannot complete life history, and cannot continue to reproduce.
Arbuscular mycorrhiza is the most common endophytic mycorrhiza, and is a symbiont formed by arbuscular mycorrhizal fungi and plant roots in soil. Arbuscular mycorrhizal fungi are widely distributed in nature, and it is known that about 90% of the flowering plants as well as ferns and bryophytes in the world can be symbiotic with arbuscular mycorrhizal fungi.
The arbuscular mycorrhizal fungi play an important role in balancing plant nutrient circulation and effectively utilizing water, can promote plant growth, improve the survival rate of transplanted plants and improve the stress resistance of the plants. Moreover, there are many beneficial effects on plant growth and development, especially in improving plant phosphorus, nitrogen and mineral nutrition. Researches show that the arbuscular mycorrhizal fungi inoculated under certain conditions can promote the absorption and utilization of phosphorus, zinc and copper in soil by plants, and have certain effects on the absorption of nitrogen, potassium, magnesium, sulfur, manganese and the like. Therefore, the arbuscular mycorrhizal fungi have wide application prospects in the fields of agriculture, forestry production and landscaping.
However, arbuscular mycorrhizal fungi are strict symbiotic fungi, and the survival of the arbuscular mycorrhizal fungi depends on living higher plants strictly, so that the difficulty in culturing and producing the arbuscular mycorrhizal fungi agent on a large scale is higher. There are many methods for obtaining arbuscular mycorrhizal fungi in the laboratory, such as: a culture medium culture method, a static nutrient solution culture method, a flowing nutrient solution culture method, a spray liquid culture method, an aseptic double culture method of arbuscular mycorrhizal fungi and isolated root organs of plants, a glass bead chamber culture method and the like, but the methods are difficult to realize continuous culture.
Disclosure of Invention
The invention aims to provide a continuous propagation incubator for arbuscular mycorrhizal fungi, which can continuously acquire hypha, spores and the like of the arbuscular mycorrhizal fungi within a period of time, and has the advantages of simple and convenient acquisition method and high efficiency.
The technical scheme adopted by the invention for realizing the technical purpose is as follows: the utility model provides a bush mycorrhizal fungi continuous type propagation incubator, includes plant incubator and sets up the hypha propagation incubator in its one side, wherein, the middle part of plant incubator has seed support gauze, and this seed support gauze separates plant incubator inside into the plant growth district on upper portion and the root system development district of lower part, it has a plurality of root hypha to breed the diffusion passageway to gather in the root system development district, and the one end of each hypha breeds the diffusion passageway is in the root system development district, and the other end stretches into in the hypha propagation incubator after passing the lateral wall of plant incubator;
the top of the hypha propagation box is open, the bottom of the hypha propagation box is of a V-shaped structure formed by two inclined bottom plates in a surrounding mode, a matrix discharge port is formed in the bottommost part of the V-shaped structure, a partition plate capable of being drawn out is horizontally arranged in the middle of the V-shaped structure, the partition plate divides the inside of the hypha propagation box into a ventilating cavity communicated with the matrix discharge port and a hypha propagation area above the partition plate, and a matrix formed by mixing glass beads and river sand in a volume ratio of 1:2 is filled in the hypha propagation area.
As a preferable scheme of the continuous propagation expansion incubator, the partition plate is a porous ceramic plate to improve the air permeability of the substrate in the hypha propagation area.
As another preferred scheme of the continuous propagation-expanding incubator, the hypha propagation and diffusion channel comprises a tube body with an opening at one end and a closed end at the other end, the part of the tube body in the root development area is a fungus penetration area, the part of the tube body in the hypha propagation and expansion area is a hypha emergence area, and through holes are densely distributed on the surfaces of the fungus penetration area and the hypha emergence area, so that root systems and arbuscular mycorrhizal fungi in the root development area enter the hypha propagation and expansion area along the inside of the tube body; the end part of the fungus pricking area is a pipe body opening end, a gauze layer is wrapped outside the fungus pricking area, and the gauze layer seals the pipe body opening end; the end part of the hypha penetrating area is closed;
a plurality of bast fiber bundles arranged along the length direction of the hypha propagation diffusion channel are distributed in a tube body of the hypha propagation diffusion channel, matrix soil is filled among the bast fiber bundles, and the matrix soil is formed by mixing vermiculite powder with the grain size not more than 1mm and river sand particles with the grain size not more than 2mm in a volume ratio of 4: 1.
As another preferable scheme of the continuous propagation expansion incubator, the diameter of the bast fiber bundle is 1-2mm, the outer diameter of each hypha propagation diffusion channel is 10-15mm, the wall thickness is 1-2mm, and the distance between adjacent hypha propagation diffusion channels is 2 cm.
As another preferable scheme of the continuous propagation culture box, the middle part of the tube body of the hypha propagation diffusion channel is a connecting area which is fixed on the side wall of the plant culture box, an inducer chamber is arranged in the side wall, an inducer is added into the inducer chamber through an inducer pipeline which extends out of the inducer chamber, the inducer is a Hoagland nutrient solution containing 5,7,4 '-trihydroxyflavone and abscisic acid, the concentration of the 5,7,4' -trihydroxyflavone is 300nmol/L, and the concentration of the abscisic acid is 700 + 800 nmol/L; the surface of the connecting area is also provided with a plurality of through holes which are used for communicating the inducer chamber with the interior of the tube body, so that the inducer enters the tube body.
As another preferred scheme of the continuous type propagation-expanding culture box, the hypha propagation diffusion channel is obliquely arranged, and the height of the same hypha propagation diffusion channel from the fungus pricking area to the hypha penetrating area is gradually reduced, so that the natural growth of the fungus hypha is facilitated.
As another preferable scheme of the continuous propagation incubator, a sealing spraying mechanism is arranged at the upper part of the hypha propagation area, the sealing spraying mechanism comprises a cover plate fixed by a clamping block arranged on the inner wall of the hypha propagation area, and the cover plate seals the hypha propagation area to form a closed chamber; a plurality of atomizing nozzles are distributed on the lower surface of the cover plate, and are communicated with a culture solution cavity in the cover plate and an external culture solution source through a culture solution pipeline; the bottom surface of the cover plate is provided with a layer of moisturizing gauze layer through a plurality of connecting rods, and the moisturizing gauze layer covers the surface of the matrix filled in the hypha propagation area.
As another preferable scheme of the continuous propagation expansion incubator, a plurality of nutrient solution tubes are distributed in a root system development area of the plant incubator, each nutrient solution tube is a strip-shaped object formed by non-woven fabric-wrapped sand stone particles, the diameter of each nutrient solution tube is 1cm, and the distance between every two adjacent nutrient solution tubes is 2-3 cm; one end of each nutrient solution pipe penetrates into a nutrient solution cavity in the side wall of the plant incubator so as to conduct the nutrient solution in the nutrient solution cavity into the root system development area; the nutrient solution cavity is positioned in the side wall of the plant incubator far away from one side of the hypha propagation expanding box and is communicated with the outside through a nutrient solution pipe so as to supplement nutrient solution into the nutrient solution pipe.
As another preferable scheme of the continuous propagation expanding incubator, a mower is arranged at the upper part of the hypha propagation expanding area, the mower is arranged on a movable base, the movable base is arranged on a slide rail on the surface of a platform plate in a sliding manner, the slide rail is parallel to the plant incubator, the movable base is driven by a motor to reciprocate along the slide rail, and plants growing to a certain height are cut off; the platform plate is fixed at the top of the hypha propagation area.
As another preferable scheme of the continuous propagation box, plant incubators are symmetrically arranged on two sides of the hypha propagation box, and the plant incubators and the hypha propagation box are both long strips.
Compared with the prior art, the invention has the following beneficial effects:
1) the hypha propagation box is arranged on one side of the plant culture box and is communicated with the hypha propagation and diffusion channel by utilizing the hypha propagation and diffusion channel, so that arbuscular mycorrhizal fungi and fibrous roots of plants enter the hypha propagation and diffusion channel and grow along the channel to enter a matrix in the hypha propagation and expansion box, the bottom of the hypha propagation and expansion box is of a V-shaped structure surrounded by two inclined bottom plates, a matrix discharge port is formed at the bottommost part of the V-shaped structure, a partition plate capable of being drawn out is horizontally arranged in the middle of the V-shaped structure and divides the inside of the hypha propagation and expansion box into a permeable cavity communicated with the matrix discharge port and a hypha propagation area above the partition plate, and the hypha propagation and expansion area is filled with the matrix formed by mixing glass beads and river sand in a volume ratio of 1; by adopting the structural design, the substrate containing the arbuscular mycorrhizal fungi hypha and the spores can be conveniently led out to be prepared into the arbuscular mycorrhizal fungi microbial inoculum, and a new substrate is added into the hypha propagation box, so that the continuous propagation is realized;
2) the main body of the hypha propagation diffusion channel is a tube body with through holes distributed on the surface, and the gauze layer coated on the surface of the through holes on the fungus stabbing area positioned in the root system development area can prevent soil in the root system development area from blocking the through holes, but can enable plant fibrous root systems and arbuscular mycorrhizal fungi to enter the hypha propagation diffusion channel through the through holes and enter a substrate of the hypha propagation area through the hypha piercing area, and then after a period of culture, a large amount of hypha and spores exist in the substrate, after the substrate is discharged through a substrate discharge port, a new substrate is put into the hypha propagation box from the upper end, and then a new round of propagation can be performed;
3) the substrate soil formed by mixing the bast fiber bundles, the vermiculite powder and the river sand particles is filled in the hypha breeding diffusion channel, and the existence of the bast fiber bundles in the substrate soil can form a nutrient solution extending along the length direction of the hypha breeding diffusion channel and a hypha growth channel, so that arbuscular mycorrhizal fungi are expanded and propagated along the direction of the bast fiber bundles, and then enter the substrate of a hypha propagation region within the shortest time;
4) the connecting area in the middle of the hypha breeding diffusion channel is communicated with the inducer cavity through the through hole, so that an inducer in the inducer cavity can enter the hypha breeding diffusion channel, the hypha breeding diffusion channel is inclined, and a phloem fiber bundle is arranged in the hypha breeding diffusion channel, so that the inducer can diffuse towards the part of the hypha passing-out area, the concentration of the inducer in the area is improved, the main body of the inducer is Hoagland nutrient solution, necessary nutrient elements are provided for the growth of arbuscular mycorrhizal fungi and the root system, abscisic acid and 5,7,4' -trihydroxyflavone contained in the guiding area can accelerate the growth of the arbuscular mycorrhizal fungi and accelerate the spore division of the arbuscular mycorrhizal fungi, and the propagation speed is improved;
5) the root system development area is internally provided with a plurality of nutrient solution pipes, the main bodies of the nutrient solution pipes are strips formed by sand stone particles wrapped by non-woven fabrics, and the sand stone particles have high porosity and one end of the sand stone particles is positioned in the nutrient solution cavity, so that the nutrient solution in the nutrient solution cavity can be sucked into the root system development area, the nutrient solution in the soil in the root system development area is kept at a certain content, and the continuous and healthy growth of the root system is promoted.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the present invention after planting a plant;
FIG. 3 is an enlarged partial schematic view of FIG. 2;
FIG. 4 is an overall view of the exterior of the hypha propagation diffusion channel;
FIG. 5 is a schematic sectional view of a diffusion channel for hypha propagation;
FIG. 6 is a schematic top view of the structure of FIG. 1;
FIG. 7 is a schematic view of the mower of FIG. 2 with the mower added;
reference numerals: 1. the plant cultivation box comprises a plant cultivation box body 101, seed supporting gauze 102, a plant growth area 103, a root system development area 104, a nutrient solution cavity 105, a nutrient solution pipe 106, an inducer cavity 107, an inducer pipeline 2, a hypha propagation box 201, an inclined bottom plate 202, a partition plate 203, a ventilation cavity 204, a substrate discharge port 205, a hypha propagation area 206, a fixture block 3, a sealed spraying mechanism 301, a cover plate 302, a culture solution pipeline 303, a culture solution cavity 304, an atomizing nozzle 305, a moisture-preserving gauze layer 4, a hypha propagation channel 401, a fungus penetration area 402, a connection area, hypha penetrating areas 404, a through hole 405, a gauze layer 406, a bast fiber bundle 407, matrix soil 5, a nutrient solution pipe 403, 6, a mower 601, a moving base 602 and a platform plate.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following specific embodiments. The parts of the invention not illustrated in the following examples, such as the soil composition, the type of plant to be grown, the nutrient composition, the Hoagland's nutrient solution, the nutrient composition, the seed planting, the method of inoculating arbuscular mycorrhizal fungi, the time of plant cultivation, etc., within the plant incubator 1 are all those skilled in the art capable of selecting according to the prior art.
Example 1
As shown in fig. 1 and 2, a continuous type propagation expanding incubator for arbuscular mycorrhizal fungi comprises a plant incubator 1 and a hypha propagation expanding incubator 2 arranged on one side of the plant incubator 1, wherein a seed supporting gauze 101 is arranged in the middle of the plant incubator 1, the inside of the plant incubator 1 is divided into an upper plant growth area 102 and a lower root system development area 103 by the seed supporting gauze 101, a plurality of hypha propagation and diffusion channels 4 are densely distributed in the root system development area 103, one end of each hypha propagation and diffusion channel 4 is positioned in the root system development area 103, and the other end of each hypha propagation and diffusion channel extends into the hypha propagation expanding incubator 2 after penetrating through the side wall of the plant incubator 1;
the top of the hypha propagation box 2 is open, the bottom of the hypha propagation box is a V-shaped structure formed by two inclined bottom plates 201 in a surrounding mode, a substrate discharge port 204 is formed in the bottommost portion of the V-shaped structure, a partition plate 202 capable of being drawn out is horizontally arranged in the middle of the V-shaped structure, the partition plate 202 divides the inside of the hypha propagation box 2 into a ventilating cavity 203 communicated with the substrate discharge port 204 and a hypha propagation area 205 above the partition plate 202, and the hypha propagation area 205 is filled with a substrate formed by mixing glass beads and river sand in a volume ratio of 1: 2.
In this embodiment, the so-called seed supporting gauze 101 functions to allow the sown plant seeds to be located on the surface of the gauze without affecting the downward growth of the root system of the seeds;
in this embodiment, soil is filled in the root development area 103, then the surface is covered with the seed supporting gauze 101, the seeds to be sown are mixed with arbuscular mycorrhizal fungi and then are sown on the seed supporting gauze 101, and then a layer of soil is attached, so that sowing is completed;
in order to promote the root system to grow well, the culture soil filled in the root system development area 103 is formed by mixing peat soil and vermiculite particles with the particle size not more than 2mm in a volume ratio of 4:1, and is sterilized and disinfected by high-temperature steam; the covering soil in the plant growth area 102 is formed by mixing sand with the grain diameter not more than 1mm and vermiculite powder in a volume ratio of 2:3, and has good water permeability and air permeability;
in the present embodiment, the top opening of the hypha propagation box 2 is mainly used for placing new substrate therein, but may be closed by a cover plate, but the cover plate can be opened so that new substrate is injected again after the substrate in the hypha propagation area 205 is discharged;
in this embodiment, a latch is provided on the inner wall of the middle of the V-shaped structure, and the free end of the partition 202 is supported on the latch.
The foregoing is a basic embodiment of the present invention, and further modifications, optimizations and limitations can be made on the foregoing, so as to obtain the following examples:
example 2
This embodiment is an improved scheme based on embodiment 1, and the main structure thereof is the same as embodiment 1, and the improvement point is that: the partition plate 202 is a porous ceramic plate to improve the air permeability of the substrate in the hypha propagation area 205.
In this embodiment, in order to control the growth conditions in the hypha propagation area 205, a ventilation tube may be provided through the inclined bottom plate 201 above the partition plate 202 to supplement air into the substrate or to control the dryness of the substrate to promote the generation of spores.
Example 3
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 4 and 5, the hypha propagation diffusion channel 4 includes a tube body with one open end and the other closed end, the part of the tube body located in the root development area 103 is a fungus penetration area 401, the part located in the hypha propagation expansion area 205 is a hypha emergence area 403, and through holes 404 are densely distributed on the surfaces of the fungus penetration area 401 and the hypha emergence area 403, so that root systems and arbuscular mycorrhizal fungi in the root development area 103 enter the hypha propagation expansion area 205 along the inside of the tube body; the end part of the fungus penetration area 401 is a pipe body opening end, a gauze layer 405 is wrapped outside the fungus penetration area 401, and the opening end of the pipe body is plugged by the gauze layer 405; the end of the hypha penetration area 403 is closed;
a plurality of bast fiber bundles 406 arranged along the length direction of the hypha propagation diffusion channel 4 are distributed in the tube body of the hypha propagation diffusion channel 4, matrix soil 407 is filled among the bast fiber bundles 406, and the matrix soil 407 is formed by mixing vermiculite powder with the grain diameter not more than 1mm and river sand particles with the grain diameter not more than 2mm in a volume ratio of 4: 1.
Example 4
This embodiment is an improved scheme based on embodiment 3, and the main structure thereof is the same as embodiment 3, and the improvement point is that: as shown in FIGS. 4 and 5, the diameter of the bast fiber bundle 406 is 1-2mm, the outer diameter of the tube body of each hypha propagation diffusion channel 4 is 10-15mm, the wall thickness is 1-2mm, and the distance between adjacent hypha propagation diffusion channels 4 is 2 cm. The bast fiber bundles 406 are formed by binding a plurality of flax fibers.
Example 5
The present embodiment is another modified scheme based on embodiment 3, and the main structure of the present embodiment is the same as embodiment 3, and the improvement point is that: as shown in fig. 3, 4 and 5, the middle part of the tube body of the hypha propagation diffusion channel 4 is a connection area 402, the connection area 402 is fixed on the side wall of the plant incubator 1, an inducer chamber 106 is arranged in the side wall, an inducer is added into the inducer chamber 106 through an inducer pipeline 107 extending out of the side wall, the inducer is a Hoagland nutrient solution containing 5,7,4 '-trihydroxyflavone and abscisic acid, the concentration of the 5,7,4' -trihydroxyflavone is 300nmol/L, and the concentration of the abscisic acid is 700 + 800 nmol/L; the surface of the connecting region 402 is also provided with a plurality of through holes 404, and the through holes 404 communicate the inducer chamber 106 with the interior of the tube, thereby allowing the inducer to enter the tube.
Example 6
The present embodiment is another modified scheme based on embodiment 3, and the main structure of the present embodiment is the same as embodiment 3, and the improvement point is that: as shown in fig. 1, 2 and 3, the hypha propagation diffusion channel 4 is obliquely arranged, and the height of the same hypha propagation diffusion channel 4 gradually decreases from the fungus penetration zone 401 to the hypha penetration zone 403, so that the natural growth of the fungus hypha is facilitated.
In this embodiment, the inclination angle of the hypha propagation diffusion channel 4 is generally 10 to 30 ° from the horizontal plane.
Example 7
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 1, 2, 3 and 6, the upper part of the hypha propagation expanding region 205 is provided with a sealing spraying mechanism 3, the sealing spraying mechanism 3 comprises a cover plate 301 fixed by a fixture block 206 arranged on the inner wall of the hypha propagation expanding region 205, and the cover plate 301 seals the hypha propagation expanding region 205 to form a closed chamber; a plurality of atomizing nozzles 304 are distributed on the lower surface of the cover plate 301, and the atomizing nozzles 304 are all communicated with a culture solution cavity 303 in the cover plate 301 and are communicated with an external culture solution source through a culture solution pipeline 302; a layer of moisturizing gauze layer 305 is arranged on the bottom surface of the cover plate 301 through a plurality of connecting rods, and the moisturizing gauze layer 305 covers the surface of the substrate filled in the hypha propagation area 205.
Example 8
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 1, 2, 3 and 6, a plurality of nutrient solution tubes 5 are distributed in a root system development area 103 of the plant incubator 1, each nutrient solution tube 5 is a strip-shaped object formed by sand stone particles wrapped by non-woven fabrics, the diameter of the strip-shaped object is 1cm, and the distance between every two adjacent nutrient solution tubes 5 is 2-3 cm; one end of each nutrient solution pipe 5 penetrates into the nutrient solution cavity 104 in the side wall of the plant incubator 1 so as to conduct the nutrient solution in the nutrient solution cavity 104 into the root system development area 103; the nutrient solution cavity 104 is positioned in the side wall of the plant incubator 1 far away from the side of the hypha propagation expanding box 2 and is communicated with the outside through a nutrient solution pipe 105 so as to supplement nutrient solution into the nutrient solution cavity.
In this embodiment, the diameter of the sand particles does not exceed 2 mm.
Example 9
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 7, a mower 6 is provided on the top of the hypha propagation area 205, the mower 6 is provided on a movable base 601, the movable base 601 is slidably provided on a slide rail on the surface of a platform plate 602, the slide rail is parallel to the plant incubator 1, the movable base 601 is driven by a motor to reciprocate along the slide rail, and plants grown to a certain height are cut off; the platform plate 602 is fixed on the top of the hypha propagation area 205.
The arrangement of the mower 6 is added in the embodiment, so that the method is suitable for the situation that the cultivated plants are alfalfa, because the alfalfa grows for a long period, and grows luxuriantly under appropriate conditions, the ground part grows too fast, and regular pruning is needed.
Example 10
The present embodiment is another modified scheme based on embodiment 1, and the main structure of the present embodiment is the same as that of embodiment 1, and the improvement point is that: as shown in fig. 2 and 6, plant cultivation boxes 1 are symmetrically arranged on both sides of the hypha propagation box 2, and the plant cultivation boxes 1 and the hypha propagation box 2 are both long strips.
In this implementation, the plant cultivation box 1 and the hypha propagation box 2 are arranged in a long strip shape, the purpose is to obtain a substrate containing arbuscular mycorrhizal fungi hypha and spores as much as possible at one time, two corresponding plant cultivation boxes 1 are arranged on two sides of one hypha propagation box 2, the purpose is to utilize the hypha propagation and diffusion channel 4 to introduce and gather plant fibrous roots of the two plant cultivation boxes 1 into the same hypha propagation box 2, and therefore the content of the arbuscular mycorrhizal fungi in a unit volume is improved.
Claims (10)
1. The utility model provides an arbuscular mycorrhizal fungi continuous type expands numerous incubator, includes plant incubator (1) and sets up hypha in its one side and expands numerous case (2), and wherein, the middle part of plant incubator (1) has seed support gauze (101), and this seed support gauze (101) are with plant incubator (1) internal separation for plant growth district (102) on upper portion and root system development district (103) of lower part, its characterized in that: a plurality of hypha breeding diffusion channels (4) are densely distributed in the root system development area (103), one end of each hypha breeding diffusion channel (4) is positioned in the root system development area (103), and the other end of each hypha breeding diffusion channel penetrates through the side wall of the plant incubator (1) and then extends into the hypha propagation box (2);
the top of the hypha propagation box (2) is open, the bottom of the hypha propagation box is of a V-shaped structure surrounded by two inclined bottom plates (201), a substrate discharge port (204) is formed at the bottommost part of the V-shaped structure, a partition plate (202) capable of being drawn out is horizontally arranged in the middle of the V-shaped structure, the partition plate (202) divides the inside of the hypha propagation box (2) into a ventilating cavity (203) communicated with the substrate discharge port (204) and a hypha propagation area (205) above the partition plate (202), and the hypha propagation area (205) is filled with a substrate formed by mixing glass beads and river sand in a volume ratio of 1: 2.
2. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 1, wherein: the clapboard (202) is a porous ceramic plate to improve the air permeability of the substrate in the hypha propagation area (205).
3. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 1, wherein: the hypha breeding diffusion channel (4) comprises a pipe body with an opening at one end and a closed end, the part of the pipe body, which is positioned in the root system development area (103), is a fungus penetration area (401), the part, which is positioned in the hypha propagation area (205), is a hypha emergence area (403), and through holes (404) are densely distributed on the surfaces of the fungus penetration area (401) and the hypha emergence area (403), so that root systems and arbuscular mycorrhizal fungi in the root system development area (103) enter the hypha propagation area (205) along the inside of the pipe body; the end part of the fungus pricking area (401) is a pipe body opening end, a gauze layer (405) wraps the outside of the fungus pricking area (401), and the pipe body opening end is plugged by the gauze layer (405); the end part of the hypha penetrating area (403) is closed;
a plurality of bast fiber bundles (406) arranged along the length direction of the hypha propagation diffusion channel (4) are distributed in the tube body of the hypha propagation diffusion channel, matrix soil (407) is filled among the bast fiber bundles (406), and the matrix soil (407) is formed by mixing vermiculite powder with the grain size not more than 1mm and river sand particles with the grain size not more than 2mm in a volume ratio of 4: 1.
4. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 3, wherein: the diameter of the bast fiber bundle (406) is 1-2mm, the outer diameter of the tube body of each hypha breeding diffusion channel (4) is 10-15mm, the wall thickness is 1-2mm, and the distance between adjacent hypha breeding diffusion channels (4) is 2 cm.
5. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 3, wherein: the middle part of the tube body of the hypha breeding diffusion channel (4) is provided with a connecting area (402), the connecting area (402) is fixed on the side wall of the plant incubator (1), an inducer chamber (106) is arranged in the side wall, an inducer is added into the inducer chamber (106) through an inducer pipeline (107) extending out of the side wall, the inducer is a Hoagland nutrient solution containing 5,7,4 '-trihydroxyflavone and abscisic acid, the concentration of the 5,7,4' -trihydroxyflavone is 300nmol/L, and the concentration of the abscisic acid is 700 + 800 nmol/L; the surface of the connecting region (402) is also provided with a plurality of through holes (404), and the through holes (404) communicate the inducer chamber (106) with the interior of the tube body, so that the inducer enters the tube body.
6. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 3, wherein: the hypha breeds diffusion passageway (4) slope setting, and same hypha breeds diffusion passageway (4) and pricks district (401) from the fungus and wear out the height of district (403) and reduce gradually to the hypha to be convenient for the natural growth of fungus hypha.
7. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 1, wherein: the upper part of the hypha propagation area (205) is provided with a sealing spraying mechanism (3), the sealing spraying mechanism (3) comprises a cover plate (301) which is fixed by a clamping block (206) arranged on the inner wall of the hypha propagation area (205), and the cover plate (301) seals the hypha propagation area (205) to form a closed chamber; a plurality of atomizing spray heads (304) are distributed on the lower surface of the cover plate (301), and the atomizing spray heads (304) are communicated with a culture solution cavity (303) in the cover plate (301) and communicated with an external culture solution source through culture solution pipelines (302); a layer of moisturizing gauze layer (305) is arranged on the bottom surface of the cover plate (301) through a plurality of connecting rods, and the moisturizing gauze layer (305) covers the surface of the substrate filled in the hypha propagation area (205).
8. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 1, wherein: a plurality of nutrient liquid tubes (5) are distributed in a root system development area (103) of the plant incubator (1), each nutrient liquid tube (5) is a strip-shaped object formed by non-woven fabric-wrapped sand particles, the diameter of each nutrient liquid tube is 1cm, and the distance between every two adjacent nutrient liquid tubes (5) is 2-3 cm; one end of each nutrient solution pipe (5) penetrates into a nutrient solution cavity (104) in the side wall of the plant incubator (1) so as to conduct nutrient solution in the nutrient solution cavity (104) to the root system development area (103); the nutrient solution cavity (104) is positioned in the side wall of one side of the plant incubator (1) far away from the hypha propagation expanding box (2) and is communicated with the outside through a nutrient solution pipe (105) to supplement nutrient solution into the nutrient solution cavity.
9. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 1, wherein: the upper part of the hypha propagation area (205) is provided with a mower (6), the mower (6) is arranged on a movable base (601), the movable base (601) is arranged on a slide rail on the surface of a platform plate (602) in a sliding mode, the slide rail is parallel to the plant incubator (1), the movable base (601) is driven by a motor to move back and forth along the slide rail, and plants growing to a certain height are cut off; the platform plate (602) is fixed on the top of the hypha propagation area (205).
10. The continuous propagation incubator of arbuscular mycorrhizal fungi according to claim 1, wherein: plant incubators (1) are symmetrically arranged on two sides of the hypha propagation expanding box (2), and the plant incubators (1) and the hypha propagation expanding box (2) are both long strips.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462517A (en) * | 2021-06-09 | 2021-10-01 | 东北师范大学 | Multilayer chambered device for continuously expanding propagation of arbuscular mycorrhizal fungal spores |
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