CN109430033A - Submerged plant isotope culture apparatus and application method - Google Patents

Submerged plant isotope culture apparatus and application method Download PDF

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Publication number
CN109430033A
CN109430033A CN201811271514.5A CN201811271514A CN109430033A CN 109430033 A CN109430033 A CN 109430033A CN 201811271514 A CN201811271514 A CN 201811271514A CN 109430033 A CN109430033 A CN 109430033A
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CN
China
Prior art keywords
isotope
submerged plant
culture solution
bar
cabinet
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CN201811271514.5A
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Chinese (zh)
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CN109430033B (en
Inventor
余华
戴文红
徐力
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Hangzhou Normal University
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Hangzhou Normal University
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Publication of CN109430033A publication Critical patent/CN109430033A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Hydroponics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Submerged plant isotope culture apparatus and application method, for the convenient carry out isotope culture to submerged plant.It is equipped with case lid at the top of the box body, characterized in that further include supporting rod, culture solution bucket and isotope addition mechanism, supporting rod is equipped on the inner wall of cabinet, a pair of of grip block is fixed on supporting rod;It is fixed with water inlet pipe on the side wall of the box body, water inlet pipe connect with culture solution bucket pipe and is equipped with valve on water inlet pipe, fills culture solution in culture solution bucket;It is equipped with isotope on case lid and adds mechanism, the isotope liquid in isotope addition mechanism is sprayed on the submerged plant of the bottom of box with atomised form, is enclosed on the outer wall of lower box and is protected from light paper.The clamping to submerged plant may be implemented in the present invention, avoids the movement of submerged plant;It can according to need addition culture solution, and culture solution can be stirred;The desired amount of isotope liquid can be sprayed on submerged plant as needed, and convenient and simple for operation.

Description

Submerged plant isotope culture apparatus and application method
Technical field
The present invention relates to submerged plant culture technique field, specifically a kind of submerged plant isotope culture apparatus and Application method.
Background technique
Submerged plant root is degenerated or is completely disappeared, and moisture and nutriment soluble in water can be directly absorbed.They are The important component of Wetland ecological has the important ecological value and environmental purification function.Since submerged plant is sunk for a long time Under water, only when blooming, anthocaulus, flower just expose the surface Activities of Some Plants, grind accordingly, with respect to the stable isotope of submerged plant Study carefully and faces huge challenge.Currently, the processing of submerged plant stable isotope and addition are mainly by stablizing same position in water body addition The extensive mode such as element lacks and carries out the quantitative device applied with growth conditions observation to stable isotope.Therefore it is a kind of heavy to need Water plant isotope culture apparatus, for being somebody's turn to do to the accurate addition of submerged plant isotope and its continuous observation of Growth trends Device is easy to operate, and use is safe.
Summary of the invention
The purpose of the present invention is to provide a kind of submerged plant isotope culture apparatus and application methods, for convenient to heavy The carry out isotope culture of water plant.
The technical scheme adopted by the invention to solve the technical problem is that: submerged plant isotope culture apparatus, it includes Cabinet is equipped with case lid at the top of the box body, characterized in that further includes supporting rod, culture solution bucket and isotope addition mechanism, in case The inner wall of body is equipped with supporting rod, and a pair of of grip block is fixed on supporting rod;It is fixed with water inlet pipe on the side wall of the box body, into Water pipe connect with culture solution bucket pipe and is equipped with valve on water inlet pipe, fills culture solution in culture solution bucket;
It is equipped with isotope on case lid and adds mechanism, the isotope liquid in isotope addition mechanism is sprayed at atomised form On the submerged plant of the bottom of box, it is enclosed on the outer wall of lower box and is protected from light paper.
Further, it is equipped with the first mounting ring in the bottom of the case lid, the first mounting ring and cabinet open end screw thread connect It connects.
Further, the supporting rod includes the second bar that the first bar and the first bar are slidably connected, and two grip blocks are fixed on On second bar and grip block is elastic component, and a pair of of articulated shaft is fixed on the first bar, a pair is fixed on cabinet wall and is led Rail is equipped with vertical track groove on guide rail, and articulated shaft is placed in track groove, the first bar be placed between two guide rails and the first bar with Guide rail contact.
Further, it is fixed with security bar on the outer wall of culture solution bucket, slot is equipped on security bar, in culture solution bucket Top be rotatablely installed bung, sliding block is installed in the basal sliding of bung, is rotatably equipped with stirring sheet on sliding block, and is stirred Damping piece is equipped between piece and sliding block, sliding block, stirring sheet and shaft constitute rabbling mechanism;In use, by stirring sheet to culture The axis direction of liquid bucket is mobile and stirring sheet is placed in culture solution bucket.
Further, it is fixed with protrusion on the outer wall of bung, is equipped with straight trough in the bottom of bung, sliding block is slidably mounted on In straight trough.
Further, isotope addition mechanism includes the installation that soft bottle above case lid is arranged in, soft bottom of bottle portion is arranged in Pipe, connect and extend downwardly with installing pipe extension, be arranged on extension spray head, soft top of bottle is set buret, Valve between buret and soft bottle is set, air inlet is equipped on soft bottle, check valve is equipped in air inlet, in check valve Soft bottle can only be entered by air inlet by acting on lower gas.
Further, spray head is hemispherical dome structure, and spray orifice is equipped on spray head outer wall.
Further, it is equipped with outlet pipe in the bottom of culture solution bucket, outlet pipe connect with water inlet pipe pipe and on water inlet pipe Equipped with valve.
The application method of submerged plant isotope culture apparatus, characterized in that the following steps are included:
(1) submerged plant is clamped in cabinet;
(2) culture solution is added into cabinet;
(3) as needed, squeezing soft bottle is sprayed at the isotope liquid in soft bottle on submerged plant.
The beneficial effects of the present invention are: submerged plant isotope culture apparatus provided by the invention and application method, a side The clamping to submerged plant may be implemented in face, avoids the movement of submerged plant;It can according to need addition culture solution, and can be right Culture solution is stirred;The desired amount of isotope liquid can be sprayed on submerged plant as needed, and convenient and simple for operation.
Detailed description of the invention
Fig. 1 is front schematic view of the invention;
Fig. 2 is the schematic cross-sectional view of cabinet;
Fig. 3 is the schematic top plan view of case lid;
Fig. 4 is the scheme of installation of supporting rod;
Fig. 5 is the schematic diagram of supporting rod;
Fig. 6 is the appearance schematic diagram that isotope adds mechanism;
Fig. 7 is the schematic cross-sectional view that isotope adds mechanism;
Fig. 8 is partial enlarged view at the A in Fig. 7;
Fig. 9 is the appearance schematic diagram of culture solution bucket;
Figure 10 is the cross-sectional view of culture solution bucket;
Figure 11 is the schematic three dimensional views of bung;
Schematic diagram when Figure 12 is bung stir culture liquid;
In figure: 1 cabinet, 11 case lids, 111 the first mounting rings, 112 stepped holes, 12 are protected from light paper, 13 guide rails, 131 track grooves, 14 water inlet pipes, 15 valves, 2 first bars, 21 articulated shafts, 22 second bars, 23 grip blocks, 3 culture solution buckets, 31 outlet pipes, 32 protection Item, 321 slots, 33 stirring sheets, 34 bungs, 341 second mounting rings, 342 straight troughs, 343 protrusions, 35 handles, 36 sliding blocks, 37 is perpendicular Axis, 4 culture solutions, 5 soft bottles, 51 installing pipes, 52 extensions, 53 mounting blocks, 54 spray heads, 541 spray orifices, 542 third mounting rings, 55 amounts Pipe, 56 graduation marks, 57 filling openings, 58 air inlets.
Specific embodiment
As shown in Figures 1 to 12, submerged plant isotope culture apparatus of the invention mainly includes cabinet 1, supporting rod, training Nutrient solution bucket 3, rabbling mechanism and isotope add mechanism, and present invention will now be described in detail with reference to the accompanying drawings..
As shown in Figure 1 and Figure 2, cabinet 1 is basic components of the invention, and cabinet is the hollow structure of open top, in cabinet Top side detachable case lid 11 is installed, as shown in figure 3, case lid is circular configuration, be equipped with stepped hole at the center of case lid 112, as shown in Fig. 2, being equipped with the first mounting ring 111 in the bottom of case lid, case lid is threadedly coupled by the first mounting ring with cabinet. It is enclosed on the outer wall of lower box and is protected from light paper 12, the root for being set as submerged plant for being protected from light paper provides dark environment. It is fixed with a pair of guide rails 13 on the side wall of the box body, vertical track groove 131 is equipped on guide rail, folder is equipped between two guide rails Bar is held, as shown in Figure 4, Figure 5, supporting rod includes the first bar 2, the second bar 22, articulated shaft 21 and grip block 23, is set on the first bar There is a pair of of articulated shaft 21, articulated shaft is placed in track groove, and can be moved up and down and be rotated in track groove, the two sides of the first bar Frictional force between guide rail contact and the first bar and guide rail allows between the first bar and vertical plane in any included angle. It is slidably fitted with the second bar 22 in the inner cavity of the first bar, damping piece is equipped between the inner wall of the first bar and the second bar, to increase The frictional force of the two.A pair of arc-shaped grip block 23, grip block are fixed in the one end for the second bar being placed in outside the first bar With elasticity, when needing to clamp submerged plant, the root of submerged plant or stem are placed between two grip blocks.As needed Position on the plant of clamping, the position of up and down adjustment supporting rod.
It is fixed with water inlet pipe 14 on the side wall of the box body, water inlet pipe is connect with culture solution bucket 3.Such as Fig. 1, Fig. 9 to Figure 12 institute Show, culture solution bucket 3 be open top hollow structure, the bottom of culture solution bucket be equipped with L-shaped outlet pipe 31, outlet pipe with into Water pipe thread connection and good seal.It is fixed with security bar 32 on the outer wall of culture solution bucket, slot is equipped on security bar 321, slot runs through the top of security bar, bung 34 is equipped in the open end of culture solution bucket, as shown in figure 11, in the bottom of bung Second mounting ring 341 equipped with C-shaped, bung be rotatablely connected by second mounting ring and culture solution bucket and bung and culture solution bucket it Between good seal.Protrusion 343 is fixed on the side wall of bung, protrusion can be integrally formed with bung.It is set in the bottom of bung There is straight trough 342, sliding block 36 is slidably fitted in straight trough, vertical pivot 37 is rotatably equipped on sliding block, circle is fixed on vertical pivot The stirring sheet 33 of arc, is fixed with handle 35 on bung.When needing to be stirred the culture solution in culture solution bucket, such as scheme Shown in 12, along straight trough sliding slider, so that notch of the stirring sheet across second mounting ring both ends is placed on second mounting ring Then bung is rotatably installed on culture solution bucket by inside, hold handle rotation bung and realize to the culture solution in culture solution bucket Stirring.When not needing stir culture liquid, sliding slider makes stirring sheet be placed in the outside of second mounting ring, and bung is pacified After on culture solution bucket, stirring sheet is placed in slot, and the setting of security bar can play the role of protection, and energy to stirring sheet Prevent pollution of the dust to stirring sheet.Sliding block, vertical pivot and stirring sheet constitute rabbling mechanism, have damping between vertical pivot and sliding block Part, the setting of damping piece is so that stirring sheet will not arbitrarily rotate under flow action when bung rotates, and then guarantee the effect of stirring Fruit.
As shown in Figure 1, being equipped with isotope in the top of cabinet adds mechanism, as shown in Fig. 1, Fig. 6 to Fig. 8, isotope adds Add mechanism include soft bottle 5 above case lid is set, installing pipe 51 that soft bottom of bottle portion is set, be arranged below installing pipe and with The extension 52 of installing pipe connection, passes through third mounting ring 542 and mounting blocks screw thread at the mounting blocks 53 that extension bottom of the tube is arranged in The spray head 54 of connection, the buret 55 that soft top of bottle is set, the filling opening 57 being arranged at the top of buret, be arranged on soft bottle into Port 58, the check valve being arranged in air inlet, soft bottle have elasticity, can be under own resilient effect after soft bottle stress deformation Restore to the original state quickly, air inlet is connected to soft bottle inner cavity, the setting of air inlet internal check valve so that gas can only by air inlet into Enter in soft bottle, and cannot be flowed out out of soft bottle through air inlet.Installing pipe is connect with the stepped hole screw thread on case lid.Extension can be with For sebific duct, the effect of extension is to make spray head close to submerged plant by increasing length.As shown in figure 8, spray head is hemispherical Hollow structure, on the outer wall of spray head be equipped with several spray orifices 541.It is equipped with valve 15 in the lower part of buret, in the outer wall of buret It is equipped with graduation mark 56.By the way that isotope liquid is added in filling opening vector tube when use, the graduation mark on buret is observed with determination The volume of the isotope liquid in buret is added;When paying attention to that isotope liquid is added in vector tube, the valve of buret lower part should be closed. After the isotope liquid for determining volume is added in vector tube, opens valve and isotope liquid is entered in soft bottle, one in soft bottle Divide isotope liquid then to enter in installing pipe, extension and spray head, squeezes soft bottle with hand at this time and force its deformation, the same position in spray head Plain liquid is fallen on submerged plant through spray orifice ejection.Then hand is unclamped, soft bottle restores under natural resiliency effect at this time, extraneous sky Gas then enters in soft bottle through air inlet.Then soft bottle is squeezed again, can continue to spray the isotope liquid mistization in spray head.
For convenience of control, valve also is provided on water inlet pipe, to facilitate on-off pipeline.
The application method of submerged plant isotope culture apparatus, comprising the following steps:
(1) submerged plant is clamped in cabinet;
(2) culture solution is added into cabinet;
(3) as needed, squeezing soft bottle is sprayed at the isotope liquid in soft bottle on submerged plant.

Claims (9)

1. submerged plant isotope culture apparatus, it includes cabinet, is equipped with case lid at the top of the box body, characterized in that further include Supporting rod, culture solution bucket and isotope add mechanism, and supporting rod is equipped on the inner wall of cabinet, a pair is fixed on supporting rod Grip block;It is fixed with water inlet pipe on the side wall of the box body, water inlet pipe connect with culture solution bucket pipe and is equipped on water inlet pipe valve, Culture solution is filled in culture solution bucket;
It is equipped with isotope on case lid and adds mechanism, the isotope liquid in isotope addition mechanism is sprayed at cabinet with atomised form On the submerged plant of bottom, it is enclosed on the outer wall of lower box and is protected from light paper.
2. submerged plant isotope culture apparatus according to claim 1, characterized in that be equipped in the bottom of the case lid The first mounting ring, the first mounting ring are threadedly coupled with cabinet open end.
3. submerged plant isotope culture apparatus according to claim 1, characterized in that the supporting rod includes first Bar, the second bar being slidably connected with the first bar, two grip blocks are fixed on the second bar and grip block is elastic component, on the first bar It is fixed with a pair of of articulated shaft, a pair of guide rails is fixed on cabinet wall, vertical track groove is equipped on guide rail, articulated shaft is set In in track groove, the first bar is placed between two guide rails and the first bar and guide rail contact.
4. submerged plant isotope culture apparatus according to claim 1, characterized in that solid on the outer wall of culture solution bucket Surely there is security bar, slot is equipped on security bar, be rotatablely installed bung at the top of culture solution bucket, pacify in the basal sliding of bung Equipped with sliding block, stirring sheet is rotatably equipped on sliding block, and between stirring sheet and sliding block be equipped with damping piece, sliding block, stirring sheet and Shaft constitutes rabbling mechanism;In use, to the axis direction movement of culture solution bucket and stirring sheet is made to be placed in training stirring sheet In nutrient solution bucket.
5. submerged plant isotope culture apparatus according to claim 4, characterized in that be fixed on the outer wall of bung Protrusion is equipped with straight trough in the bottom of bung, and sliding block is slidably mounted in straight trough.
6. submerged plant isotope culture apparatus according to claim 1, characterized in that isotope addition mechanism includes setting Set the soft bottle above case lid, the installing pipe that soft bottom of bottle portion is set, the extension for connecting and extending downwardly with installing pipe, setting In spray head, the buret that soft top of bottle is arranged in, the valve that is arranged between buret and soft bottle on extension, it is equipped on soft bottle Air inlet is equipped with check valve in air inlet, and gas can only enter soft bottle by air inlet under the action of check valve.
7. submerged plant isotope culture apparatus according to claim 6, characterized in that spray head is hemispherical dome structure, Spray head outer wall is equipped with spray orifice.
8. submerged plant isotope culture apparatus according to claim 1, characterized in that be equipped in the bottom of culture solution bucket Outlet pipe, outlet pipe connect with water inlet pipe pipe and are equipped with valve on water inlet pipe.
9. the application method of any submerged plant isotope culture apparatus of claim 1 to 8, characterized in that including with Lower step:
(1) submerged plant is clamped in cabinet;
(2) culture solution is added into cabinet;
(3) as needed, squeezing soft bottle is sprayed at the isotope liquid in soft bottle on submerged plant.
CN201811271514.5A 2018-10-29 2018-10-29 Submerged plant isotope culture device and use method thereof Active CN109430033B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811271514.5A CN109430033B (en) 2018-10-29 2018-10-29 Submerged plant isotope culture device and use method thereof

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Application Number Priority Date Filing Date Title
CN201811271514.5A CN109430033B (en) 2018-10-29 2018-10-29 Submerged plant isotope culture device and use method thereof

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CN109430033A true CN109430033A (en) 2019-03-08
CN109430033B CN109430033B (en) 2024-05-14

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110161146A (en) * 2019-06-14 2019-08-23 扬州大学 A kind of experimental provision and its method of measurement paddy field drainage irrigation canals and ditches greenhouse gases layering flux

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CN203152174U (en) * 2013-03-24 2013-08-28 温茜茜 Multi-purpose type manual type irrigation bottle
TWM483027U (en) * 2014-02-17 2014-08-01 ji-chang Yang The coffee mug handle combines with a stir stick
CN104258498A (en) * 2014-10-10 2015-01-07 济南高达信息技术有限公司 Convenient quantitative sprayer for powder drugs
CN106264201A (en) * 2016-08-24 2017-01-04 浙江健仕科技股份有限公司 A kind of pulverizing wall-breaking machine
CN106386451A (en) * 2016-11-02 2017-02-15 广西壮族自治区农业科学院经济作物研究所 Aeroponic cultivation device for observing growth development of plant
CN206596482U (en) * 2017-04-07 2017-10-31 刘红 Floatation type hydroponic device is used in experiment
CN107937267A (en) * 2017-11-20 2018-04-20 浙江竹孝宝生物科技有限公司 A kind of constant pressure ferment bucket
CN207913586U (en) * 2018-01-26 2018-09-28 洛阳毅航耐火材料有限公司 A kind of alcohol-base foundry coating is convenient for handling storage and transportation apparatus
CN207995658U (en) * 2018-03-22 2018-10-23 中国农业科学院北京畜牧兽医研究所 The portable classification inoculation apparatus in field
CN209201753U (en) * 2018-10-29 2019-08-06 杭州师范大学 Submerged plant isotope culture apparatus

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JPH03272624A (en) * 1990-03-23 1991-12-04 Fujita Corp Apparatus for cultivating plant
CN101015248A (en) * 2006-07-28 2007-08-15 中国科学院亚热带农业生态研究所 Method and device for plants carbon isotope labelling
CN201630853U (en) * 2010-03-25 2010-11-17 王丝雯 Water cup with stirring rod
KR20130009505A (en) * 2011-07-15 2013-01-23 경기도 Plant cultivation device with growing bed of double plate structure
CN203152174U (en) * 2013-03-24 2013-08-28 温茜茜 Multi-purpose type manual type irrigation bottle
TWM483027U (en) * 2014-02-17 2014-08-01 ji-chang Yang The coffee mug handle combines with a stir stick
CN104258498A (en) * 2014-10-10 2015-01-07 济南高达信息技术有限公司 Convenient quantitative sprayer for powder drugs
CN106264201A (en) * 2016-08-24 2017-01-04 浙江健仕科技股份有限公司 A kind of pulverizing wall-breaking machine
CN106386451A (en) * 2016-11-02 2017-02-15 广西壮族自治区农业科学院经济作物研究所 Aeroponic cultivation device for observing growth development of plant
CN206596482U (en) * 2017-04-07 2017-10-31 刘红 Floatation type hydroponic device is used in experiment
CN107937267A (en) * 2017-11-20 2018-04-20 浙江竹孝宝生物科技有限公司 A kind of constant pressure ferment bucket
CN207913586U (en) * 2018-01-26 2018-09-28 洛阳毅航耐火材料有限公司 A kind of alcohol-base foundry coating is convenient for handling storage and transportation apparatus
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CN209201753U (en) * 2018-10-29 2019-08-06 杭州师范大学 Submerged plant isotope culture apparatus

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110161146A (en) * 2019-06-14 2019-08-23 扬州大学 A kind of experimental provision and its method of measurement paddy field drainage irrigation canals and ditches greenhouse gases layering flux
CN110161146B (en) * 2019-06-14 2021-10-01 扬州大学 Experimental device and method for measuring layered flux of greenhouse gases in drainage ditch of rice field

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