CN116840002A - Analysis method of carbon dioxide emission source of mangrove seedling sediment-atmosphere interface - Google Patents
Analysis method of carbon dioxide emission source of mangrove seedling sediment-atmosphere interface Download PDFInfo
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 title claims abstract description 196
- 239000001569 carbon dioxide Substances 0.000 title claims abstract description 98
- 229910002092 carbon dioxide Inorganic materials 0.000 title claims abstract description 98
- 240000002044 Rhizophora apiculata Species 0.000 title claims abstract description 70
- 238000004458 analytical method Methods 0.000 title abstract description 11
- OKTJSMMVPCPJKN-OUBTZVSYSA-N Carbon-13 Chemical compound [13C] OKTJSMMVPCPJKN-OUBTZVSYSA-N 0.000 claims abstract description 59
- 239000013049 sediment Substances 0.000 claims abstract description 48
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 238000010586 diagram Methods 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims description 26
- 230000006907 apoptotic process Effects 0.000 claims description 16
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 238000012417 linear regression Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000000155 isotopic effect Effects 0.000 description 2
- 241001635619 Avicennia marina Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
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Abstract
The invention discloses an analysis method of carbon dioxide emission sources of a mangrove seedling sediment-atmosphere interface, which comprises the steps of carrying out carbon 13 isotope enrichment on mangrove seedlings in a cultivation box provided with a littering object collection net and a collection net, then measuring carbon dioxide emission and carbon 13 isotopes on the surface of a target mangrove seedling sediment, and respectively calculating carbon dioxide emission and carbon 13 isotope values of the sediment-atmosphere interface through an ideal gas state equation and a Keeling diagram; the method combines the organic carbon 13 isotopes of the falling matters, the fine roots and the sediments, establishes an isotope mixing model, calculates the contribution of the falling matters, the fine roots and the sediments to the carbon dioxide emission of the sediment-atmosphere interface, and solves the problem of the prior art that the analysis of the carbon dioxide emission sources of the mangrove seedling sediment-atmosphere interface is difficult by actually measuring the carbon dioxide emission and the carbon 13 isotopes of each source and combining the isotope mixing model.
Description
Technical field:
the invention relates to the fields of forestry technology and ecological protection, in particular to a method for analyzing carbon dioxide emission sources at a mangrove seedling sediment-atmosphere interface.
The background technology is as follows:
mangrove is a woody plant-based community that grows in the intertidal zone of tropical or subtropical coasts or estuary areas. From the perspective of global mangrove carbon recycling, about half of the carbon is unknown, and carbon dioxide emissions from the sediment are an important component of the unknown carbon. Large-scale mangrove ecological restoration requires planting a large number of mangrove seedlings, improving knowledge of carbon source and sink of the mangrove and improving carbon fixing capacity of the mangrove, and urgent need is to clear carbon dioxide emission sources of the mangrove seedlings.
Carbon dioxide emissions at the mangrove sediment-atmosphere interface may result from fine root respiration and microbial decomposition of the litter and organic matter in the sediment, and from a domestic to foreign perspective, carbon 13 isotope technology has been widely used for the traceability of mangrove sediment organics and the research of food nets. However, there is currently no patent literature providing a method for quantitatively resolving the carbon dioxide emissions sources at the mangrove seedling deposit-atmosphere interface. The carbon 13 isotope values of the litters and the fine roots of the mangrove plants are close, the natural carbon 13 isotopes cannot distinguish the contribution of the litters and the fine roots to the carbon dioxide emission of the mangrove sediment-atmosphere interface, and the contribution of the litters and the fine roots to the carbon dioxide emission needs to be separated through a carbon 13 isotope enrichment method. Therefore, development and innovation are needed.
The invention comprises the following steps:
the invention aims to provide an analysis method of carbon dioxide emission sources of a mangrove seedling sediment-atmosphere interface, which comprises the steps of carrying out carbon 13 isotope enrichment on mangrove seedlings in a cultivation box provided with a littering object collection net and a collection net, then measuring carbon dioxide emission and carbon 13 isotopes on the surface of a target mangrove seedling sediment, and respectively calculating the carbon dioxide emission and the carbon 13 isotope values of the sediment-atmosphere interface through an ideal gas state equation and a Keeling diagram; and combining organic carbon 13 isotopes of the fallen substances, the fine roots and the sediments, establishing an isotope mixing model, calculating the contribution of the fallen substances, the fine roots and the sediments to the carbon dioxide emission of the sediment-atmosphere interface, and solving the problem of the prior art that the analysis of the carbon dioxide emission sources of the sediment-atmosphere interface of mangrove seedlings is difficult.
The invention is realized by the following technical scheme:
a method for resolving a mangrove seedling sediment-atmosphere interface carbon dioxide emission source, comprising the following steps:
1) In a mangrove seedling cultivation box provided with a litter collection net and a mangrove seedling cultivation box without the litter collection net, carrying out carbon 13 isotope enrichment on mangrove seedlings, then measuring carbon dioxide emission and carbon 13 isotopes on the sediment surface of the target mangrove seedlings, and respectively calculating carbon dioxide emission and carbon 13 isotope values of sediment-atmosphere interfaces through an ideal gas state equation and a Keeling diagram;
2) Collecting sediment and fine roots of mangrove seedlings in a cultivation box provided with a litter collection net and a litter collection net without the litter collection net, collecting the litter on the litter collection net, and respectively measuring carbon 13 isotopes;
3) And 2) combining carbon 13 isotopes of the sediment, the fine roots and the litters obtained in the step 2), establishing an isotope mixing model, and calculating the contribution of the litters, the fine roots and the sediment to the carbon dioxide emission of the sediment-atmosphere interface.
The analysis method of the carbon dioxide emission source of the mangrove seedling sediment-atmosphere interface specifically comprises the following steps:
1) For mangrove seedling cultivation boxes with litter collection net and without litter collection net, naH is placed in each cultivation box 13 CO 3 The open bottle of the solution is then closed, a small fan is fixed at the top of the cultivation box to keep the gas in the box uniform, and then hydrochloric acid solution is added to NaH through small holes at the top of the cultivation box every other day 13 CO 3 A bottle of the solution reacts to generate carbon 13 enriched carbon dioxide gas to perform carbon 13 isotopic enrichment on mangrove seedlings; after the enrichment of the carbon 13 is finished, the top of the box body is not closed any more, then the closed cover is buckled on the surface of the sediment, and the gas in the closed cover is collected; measuring carbon dioxide bias voltage and carbon 13 isotope in the collected gas by using a laser spectrometer, and respectively calculating carbon dioxide emission and carbon 13 isotope values of a sediment-atmosphere interface by using an ideal gas state equation and a Keeling diagram;
2) Collecting sediment and fine roots of mangrove seedlings in a mangrove seedling cultivation box with and without a litter collection net, collecting the litter on the litter collection net, and measuring carbon 13 isotopes respectively;
3) And (3) establishing an isotope mixing model by using the measured carbon 13 isotopes, and calculating to obtain contributions of the litters, the fine roots and the sediments to the carbon dioxide emission of the sediment-atmosphere interface.
In the step 1), a closed cover is buckled on the surface of the sediment, and the gas in the closed cover is collected, and the method specifically comprises the following steps: the enclosure was snapped onto the deposit surface and after 20 minutes, 30 ml of the enclosure was collected with a sampler every 10 minutes for a total of 5 times.
In the step 1), a laser spectrometer is adopted to measure the carbon dioxide bias voltage and the carbon 13 isotope in the collected gas, and the carbon dioxide emission F and the carbon 13 isotope value of the sediment-atmosphere interface are respectively calculated through an ideal gas state equation and a Keeling diagram, and the method specifically comprises the following steps: sequentially connecting the samplers to the laser spectrometer according to the time sequence of the area samples, and connecting the next sampler after the gas of the previous sampler is sucked into the laser spectrometer and then pulled out; the carbon dioxide emission was calculated for the carbon dioxide bias of the laser spectrometer according to the following formula:
wherein F is the carbon dioxide emission,for the change of the carbon dioxide bias along with the sampling time, V is the volume of the closed cover, R is an ideal gas constant, T is the air temperature during sampling, and S is the sediment surface area covered by the closed cover;
the intercept delta of the equation in the y-axis is calculated in the following linear regression equation 13 CO 2 ：
Wherein delta 13 CO 2 Carbon 13 isotope of carbon dioxide, delta, being a deposit-atmosphere interface 13 CO 2 The instrument is the carbon dioxide in each sampler measured by the laser spectrometerCarbon 13 isotope, pCO2 Atmospheric air Delta for carbon dioxide bias in the atmosphere 13 CO 2 atmosphere Is carbon dioxide isotope in the atmosphere, pCO 2 instrument For the carbon dioxide bias in each of the samplers measured by the laser spectrometer.
In particular, step 2) is specifically: collecting sediment in a mangrove seedling cultivation box with and without a litter collection net and fine roots of each mangrove seedling, collecting the litter on the litter collection net, cleaning the fine roots and the litter, placing the cleaned litter in a heating furnace, drying the litter at 60 ℃ to constant weight, grinding and sieving the sediment, removing inorganic carbon by hydrochloric acid, and respectively measuring carbon 13 isotopes of the sediment.
In particular, step 3) is specifically: for a cultivation box with litter collection net, f is calculated in the following equation set Root of Chinese character And f Deposit material It is the contribution of fines and deposits to the carbon dioxide emissions at the deposit-atmosphere interface:
δ 13 CO 2X1 ＝f root of Chinese character δ 13 C Root of Chinese character +f Deposit material δ 13 C Deposit material (3)
f Root of Chinese character +f Deposit material ＝1 (4)；
For mangrove seedling cultivation boxes without litter collection net, f is calculated in the following equation set Apoptosis article 、f Root of Chinese character And f Deposit material It is the contribution of fines and deposits to the carbon dioxide emissions at the deposit-atmosphere interface:
δ 13 CO 2X2 ＝f apoptosis article δ 13 C Apoptosis article +f Root of Chinese character δ 13 C Root of Chinese character +f Deposit material δ 13 C Deposit material (5)
f Apoptosis article +f Root of Chinese character +f Deposit material ＝1 (7)
The airtight cover is a transparent airtight container with one open end, and the top is provided with an opening so as to be convenient for the sampler to collect gas.
The beneficial effects of the invention are as follows:
1) The method can calculate the contribution of the fine roots and the sediment to the carbon dioxide emission amount by analyzing the source of the carbon dioxide emission amount of the sediment-atmosphere interface of the cultivation box without the collecting net, and is suitable for analyzing the carbon dioxide emission source in the early stage of mangrove seedling planting in ecological restoration.
2) The carbon dioxide emission F source analysis of the sediment-atmosphere interface carried out by the cultivation box without the collecting net can calculate the contribution of the fallen substances, the fine roots and the sediment to the carbon dioxide emission, and is suitable for the carbon dioxide emission source analysis in the later stage of mangrove seedling planting in ecological restoration.
3) According to the invention, only by measuring the carbon dioxide emission and the carbon 13 isotopes of the mangrove seedlings at the sediment-atmosphere interface and collecting a small amount of the falling matters, the fine roots and the sediment to measure the carbon 13 isotopes, the contribution of the falling matters, the fine roots and the sediment to the carbon dioxide emission of the sediment-atmosphere interface can be calculated almost without damaging the mangrove seedlings, and the problem of the prior art that the analysis difficulty of the carbon dioxide emission sources of the sediment-atmosphere interface of the mangrove seedlings is high is solved by actually measuring the carbon dioxide emission and the carbon 13 isotopes of each source and combining the carbon 13 isotopes with an isotope mixing model.
Description of the drawings:
FIG. 1 is a schematic representation of the carbon 13 isotopic enrichment of mangrove seedlings in example 1 of the present invention;
FIG. 2 is a schematic view showing the collection of gas in a sealed enclosure in example 1 of the present invention, wherein a) a cultivation box without a collection net is provided, and b) a cultivation box with a collection net is provided.
FIG. 3 is the carbon dioxide emissions from the sediment-atmosphere interface in example 1 of the present invention;
FIG. 4 is a delta of carbon dioxide emissions at the sediment-atmosphere interface in example 1 of the present invention 13 CO 2 ；
FIG. 5 is the apoptosis, root and sediment delta in example 1 of the present invention 13 C；
FIG. 6 is a plot of the contribution of the apoptosis, fine roots and sediment to the carbon dioxide emissions at the sediment-atmosphere interface of example 1 of the present invention.
The specific embodiment is as follows:
the following is a further illustration of the invention and is not a limitation of the invention.
Example 1: analysis method of carbon dioxide emission source of mangrove seedling sediment-atmosphere interface
The method comprises the following steps:
s1, selecting target mangrove avicennia marina seedlings to plant in two groups of cultivation boxes, wherein one group of cultivation boxes (6) are provided with litter collection nets at the height of the lower stem parts of seedling leaves, the other group of cultivation boxes (6) are not provided with litter collection nets, and each cultivation box is internally provided with 25 milliliters of NaH containing 1 mol/liter 13 CO 3 The solution is opened, then the cultivation box is closed, a small hole is reserved at the top of the box body and right above the solution bottle, hydrochloric acid solution is added into the solution bottle from the small hole through a glass pipette every other day within 45 days, carbon 13 enriched carbon dioxide gas is generated through reaction, so that carbon 13 isotope enrichment is carried out on mangrove seedlings (see figure 1), the small hole is closed when not used, and a small fan is fixed at the top of the cultivation box to keep the gas in the box uniform;
and after the enrichment of the carbon 13 is finished, the top of the box body is not sealed, and then a sealed cover is manufactured by using a transparent sealed container with one open end, wherein the open end of the sealed cover is used for collecting gas for the mangrove seedling cultivation box (X1) with the litters collecting net and the mangrove seedling cultivation box (X2) without the litters collecting net. The enclosure was fastened to the surface of the deposit, and after 20 minutes, 30 ml of the gas in the enclosure was collected by a sampler every 10 minutes (see fig. 2) for a total of 5 times. Sequentially connecting the samplers to the laser spectrometer according to the time sequence of the area samples, and connecting the next sampler after the gas of the previous sampler is sucked into the laser spectrometer and then pulled out; the carbon dioxide emission was calculated for the carbon dioxide bias of the laser spectrometer according to the following formula:
wherein F is the carbon dioxide emission,for the change of the carbon dioxide bias along with the sampling time, V is the volume of the closed cover, R is an ideal gas constant, T is the temperature of the sample, and S is the surface area of the sediment covered by the closed cover;
as a result, referring to fig. 3, the carbon dioxide emissions of the deposit-atmosphere interface of the cultivation boxes with and without litter collection net were 170.1±15.6 mmol/(square meter. Day) and 174.7 ±19.4 mmol/(square meter. Day), respectively.
The intercept delta of the equation in the y-axis is calculated in the following linear regression equation 13 CO 2 ：
Wherein delta 13 CO 2 Carbon 13 isotope of carbon dioxide, delta, being a deposit-atmosphere interface 13 CO 2 instrument For the carbon 13 isotope of carbon dioxide, pCO, in each of the samplers measured by the laser spectrometer 2 atmosphere Delta for carbon dioxide bias in the atmosphere 13 CO 2 atmosphere Is carbon dioxide isotope in the atmosphere, pCO 2 instrument For the carbon dioxide bias in each of the samplers measured by the laser spectrometer.
Results referring to FIG. 4, incubator deposit-atmosphere interface delta with and without litter collection mesh 13 CO 2 140.8%54.7%and 168.2%29.9%.
S2, collecting sediment in a cultivation box X1 with a litter collection net and a mangrove seedling cultivation box X2 without the litter collection net, collecting fine roots of each mangrove seedling, collecting the litter on the litter collection net in the cultivation box X1 with the litter collection net, cleaning the fine roots and the litter, then placing the cleaned litter in a heating furnace, drying the cleaned litter in the heating furnace to constant weight at 60 ℃, grinding and sieving the sediment, and removing inorganic matters by adopting hydrochloric acidCarbon, measurement of carbon 13 isotope delta 13 C Deposit material 、δ 13 C Root of Chinese character And delta 13 C Apoptosis article ；
Results referring to FIG. 5, litter, roots and sediment delta of a cultivation box with litter collection net 13 C is 24.7 ± 19.1%o, 556.5%o ± 303.9%o and 28.9%o ± 10.2%o respectively, and the apoptosis, root and sediment delta of the cultivation box with the litter collection net 13 C is 3.1%1.6%0.mill, 1183.9%0.3%0.mill and 90.4%17.6%0.mill respectively.
S3, building an isotope mixing model by using the measured carbon 13 isotopes, and calculating to obtain contribution f of the litters, the fine roots and the sediments to the carbon dioxide emission of the sediment-atmosphere interface Apoptosis article 、f Root of Chinese character And f Deposit material . The method comprises the following steps: for the cultivation box X1 with litter collection net, f is calculated in the following equation set Root of Chinese character And f Deposit material It is the contribution of fines and deposits to the carbon dioxide emissions at the deposit-atmosphere interface:
δ 13 CO 2X ＝f root of Chinese character δ 13 C Root of Chinese character +f Deposit material δ 13 C Deposit material (3)
f Root of Chinese character +f Deposit material ＝1 (4)
Results referring to fig. 6, the contributions of roots and sediment to the carbon dioxide emissions of the sediment-atmosphere interface were 41.5% ± 13.5% and 58.5% ± 13.5%, respectively, for the cultivation boxes with litter collection net.
For mangrove seedling cultivation box X2 without litter collection net, f is calculated in the following equation set Apoptosis article 、f Root of Chinese character And f Deposit material It is the contribution of fines and deposits to the carbon dioxide emissions at the deposit-atmosphere interface:
δ 13 CO 2X2 ＝f apoptosis article δ 13 C Apoptosis article +f Root of Chinese character δ 13 C Root of Chinese character +f Deposit material δ 13 C Deposit material (5)
f Apoptosis article +f Root of Chinese character +f Deposit material ＝1 (7)
Results referring to fig. 6, the contributions of the litter, roots and sediment to the sediment-atmosphere interface carbon dioxide emissions were 12.8% ± 2.8%, 22.1% ± 13.9% and 65.1% ± 13.5%, respectively, for the cultivation boxes without the litter collection net.
The foregoing is only a preferred embodiment of the present invention, but the scope of the claims is not limited thereto, and any person skilled in the art can equally substitute or change the technical solution and the inventive concept according to the present invention within the scope of the present invention disclosed in the present invention, and all those skilled in the art belong to the scope of the appended claims.
Claims (7)
1. The method for analyzing the carbon dioxide emission source of the mangrove seedling sediment-atmosphere interface is characterized by comprising the following steps of:
1) In a mangrove seedling cultivation box provided with a litter collection net and a mangrove seedling cultivation box without the litter collection net, carrying out carbon 13 isotope enrichment on mangrove seedlings, then measuring carbon dioxide emission and carbon 13 isotopes on the sediment surface of the target mangrove seedlings, and respectively calculating carbon dioxide emission and carbon 13 isotope values of sediment-atmosphere interfaces through an ideal gas state equation and a Keeling diagram;
2) Collecting sediment and fine roots of mangrove seedlings in a cultivation box provided with a litter collection net and without the litter collection net, collecting the litter on the litter collection net, and respectively measuring carbon 13 isotopes to obtain organic carbon 13 isotopes of the sediment, the fine roots and the litter;
3) And 2) combining carbon 13 isotopes of the sediment, the fine roots and the litters obtained in the step 2), establishing an isotope mixing model, and calculating the contribution of the litters, the fine roots and the sediment to the carbon dioxide emission of the sediment-atmosphere interface.
2. The method for resolving a source of carbon dioxide emissions at a mangrove seedling deposit-atmosphere interface of claim 1, comprising the steps of:
1) For mangrove seedling cultivation boxes with litter collection net and without litter collection net, naH is placed in each cultivation box 13 CO 3 The open bottle of the solution is then closed, a small fan is fixed at the top of the cultivation box to keep the gas in the box uniform, and then hydrochloric acid solution is added to NaH through small holes at the top of the cultivation box every other day 13 CO 3 The bottle of the solution reacts to generate carbon dioxide gas enriched with carbon 13 so as to carry out carbon 13 isotope enrichment on mangrove seedlings, and the small holes are closed when the mangrove seedlings are not used; after the enrichment of the carbon 13 is finished, the top of the box body is not closed any more, then the closed cover is buckled on the surface of sediment in the cultivation box, and the gas in the closed cover is collected; measuring carbon dioxide bias voltage and carbon 13 isotope in the collected gas by using a laser spectrometer, and respectively calculating carbon dioxide emission F and carbon 13 isotope value of a sediment-atmosphere interface through an ideal gas state equation and a Keeling diagram;
2) Collecting sediment and fine roots of mangrove seedlings in a mangrove seedling cultivation box with and without a litter collection net, collecting the litter on the litter collection net, and measuring carbon 13 isotopes respectively;
3) And (3) establishing an isotope mixing model by using the measured carbon 13 isotopes, and calculating to obtain contributions of the litters, the fine roots and the sediments to the carbon dioxide emission of the sediment-atmosphere interface.
3. The method for analyzing carbon dioxide emission sources of mangrove seedlings at a sediment-atmosphere interface according to claim 2, wherein in step 1), a closed cover is fastened on the surface of the sediment in a cultivation box, and the gas in the closed cover is collected, specifically comprising the following steps: the enclosure was snapped onto the deposit surface and after 20 minutes, 30 ml of the enclosure was collected with a sampler every 10 minutes for a total of 5 times.
4. The method for analyzing carbon dioxide emission sources of mangrove seedlings at a sediment-atmosphere interface according to claim 2, wherein in the step 1), a laser spectrometer is adopted to measure carbon dioxide bias voltage and carbon 13 isotopes in the collected gas, and the carbon dioxide emission amount F and the carbon 13 isotope value of the sediment-atmosphere interface are calculated respectively through an ideal gas state equation and a Keeling chart, and specifically comprises the following steps: sequentially connecting the samplers to the laser spectrometer according to the time sequence of the area samples, and connecting the next sampler after the gas of the previous sampler is sucked into the laser spectrometer and then pulled out; the carbon dioxide emission was calculated for the carbon dioxide bias of the laser spectrometer according to the following formula:
wherein F is the carbon dioxide emission,for the change of the carbon dioxide bias along with the sampling time, V is the volume of the closed cover, R is an ideal gas constant, T is the air temperature during sampling, and S is the sediment surface area covered by the closed cover;
the intercept delta of the equation in the y-axis is calculated in the following linear regression equation 13 CO 2 ：
Wherein delta 13 CO 2 Carbon 13 isotope of carbon dioxide, delta, being a deposit-atmosphere interface 13 CO 2 instrument For the carbon 13 isotope of carbon dioxide, pCO, in each of the samplers measured by the laser spectrometer 2 atmosphere Delta for carbon dioxide bias in the atmosphere 13 CO 2 atmosphere Is carbon dioxide isotope in the atmosphere, pCO 2 instrument For the carbon dioxide bias in each of the samplers measured by the laser spectrometer.
5. The method for analyzing carbon dioxide emission sources of mangrove seedlings at a sediment-atmosphere interface according to claim 2, wherein the step 2) is specifically as follows: collecting sediment in a mangrove seedling cultivation box with and without a litter collection net and fine roots of each mangrove seedling, collecting the litter on the litter collection net, cleaning the fine roots and the litter, placing the cleaned litter in a heating furnace, drying the litter at 60 ℃ to constant weight, grinding and sieving the sediment, removing inorganic carbon by hydrochloric acid, and respectively measuring carbon 13 isotopes of the sediment.
6. The method for resolving a carbon dioxide emission source of mangrove seedlings at a sediment-atmosphere interface according to claim 2,
the method is characterized in that the step 3) specifically comprises the following steps: for a cultivation box with litter collection net, f is calculated in the following equation set Root of Chinese character And f Deposit material It is the contribution of fines and deposits to the carbon dioxide emissions at the deposit-atmosphere interface:
δ 13 CO 2X1 ＝f root of Chinese character δ 13 C Root of Chinese character +f Deposit material δ 13 C Deposit material (3)
f Root of Chinese character +f Deposit material ＝1 (4)；
For mangrove seedling cultivation boxes without litter collection net, f is calculated in the following equation set Apoptosis article 、f Root of Chinese character And f Deposit material It is the contribution of fines and deposits to the carbon dioxide emissions at the deposit-atmosphere interface:
δ 13 CO 2X2 ＝f drop adjusting article δ 13 C Apoptosis article +f Root of Chinese character δ 13 C Root of Chinese character +f Deposit material δ 13 C Deposit material (5)
f Apoptosis article +f Root of Chinese character +f Deposit material ＝1 (7)
7. The method of claim 2, wherein the enclosure is a transparent, sealed container with one end open, and the top is open to facilitate gas collection by the sampler.
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