CN107014951A - Forest ecosystem breathes Carbon flux assay method - Google Patents
Forest ecosystem breathes Carbon flux assay method Download PDFInfo
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Abstract
The present invention provides a kind of forest ecosystem breathing Carbon flux assay method, including：Obtain the Atmospheric CO of different height in target forest layer in preset time period2Concentration and Atmospheric CO2In δ13C isotope values, and forest ecosystem breathing δ is obtained according to it13C isotope values；Obtain the CO that tree branches breathing to be measured is produced in target forest in preset time period2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C isotope values；The CO produced is breathed according to the tree branches to be measured of acquisition2In δ13The CO that C isotope values and soil respiration to be measured are produced2In δ13C isotope values, determine the proportionate relationship of each breathing component in forest ecosystem；Obtain the Carbon flux of soil to be measured；The Carbon flux of the soil to be measured of acquisition is substituted into the proportionate relationship of each breathing component in forest ecosystem, forest ecosystem breathing Carbon flux is obtained.The present invention can more accurately determine the Carbon flux change between air and plant in the forest ecosystem of MODEL OVER COMPLEX TOPOGRAPHY.
Description
Technical field
Breathed the present invention relates to forest ecosystem respiration monitoring technical field, more particularly to a kind of forest ecosystem
Carbon flux assay method.
Background technology
Carbon cycle of forest ecosystem is most important in global carbon, has not in regulation atmospheric carbon dioxide
Alternative effect.Forest ecosystem year carbon capacity is about 2.4 ± 0.4Pg, account for terrestrial ecosystems carbon capacity (2.6 ±
0.8Pg) 90%, or even than more than the carbon capacity (2.2 ± 0.2Pg) of the global ocean ecosystem.
During global carbon is studied, due to the complicated variety of terrestrial ecosystems, its Carbon flux is difficult to standard
Really measure, therefore researcher generally converges the carbon of terrestrial ecosystems and handled as the remainder of Global carbon balance equation.
And forest is due to the characteristics of its composition is various, tree crown is tall and big, geometric distortion is big, being all types of ecosystems in land again
In it is the most complicated.Therefore, the direct assessment converged for Global Forests system carbon, is still the focus and difficult point studied at present.
At present, in the method for Forest Ecosystem Carbon flux measurement, mainly include：Case method and microclimate method.
Case method is the most commonly used method in current scientific research, and its operation principle is to be covered tested region with casing,
Changes of concentrations of carbon dioxide in isolating inner air and outer air, time sight case, in the hope of carbon dioxide exchange amount in case.The letter of its method
It is single, it is with low cost.But by being limited by box volume, case method is difficult to be applied to high megaphanerophyte, more at present to be used to measure soil
The Carbon flux of breathing or short shrub is determined, and its time precision is relatively low, it is impossible to carry out long-time Continuous Observation.
Microclimate method is the gas turbulence and its changes of concentrations of carbon dioxide close to earth's surface by measurement, and then is derived
Carbon dioxide flux between vegetation air.Eddy covariance technique is long-term continuous straight with it as one of representative method of microclimate method
Obtain to obtain the advantage that Carbon flux changes under large scale, it has also become determine the standard method of carbon dioxide flux in the world at present.But
That eddy covariance technique also has the restrictive condition of implementation, it is desirable to tested region atmospheric condition be in stable state, underlying surface it is uniform,
There is no carbon exchange between underlying surface and instrument.And in actual applications, tested region tends not to be under ideal conditions, this
Also the application to eddy covariance technique proposes problem.
In consideration of it, how a kind of reliable forest ecosystem breathing Carbon flux assay method is provided, can be intricately
The Carbon flux change between air and plant is more accurately determined in the forest ecosystem of shape condition turns into what needs at present were solved
Technical problem.
The content of the invention
To solve above-mentioned technical problem, the present invention provides a kind of forest ecosystem breathing Carbon flux assay method, energy
Enough Carbon flux changes more accurately determined in the forest ecosystem of MODEL OVER COMPLEX TOPOGRAPHY between air and plant.
In a first aspect, the present invention provides a kind of forest ecosystem breathing Carbon flux assay method, including：
Obtain the Atmospheric CO of different height in target forest layer in preset time period2Concentration and Atmospheric CO2In δ13C
Isotope value；
According to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13C isotopes
Value, obtains forest ecosystem breathing δ13C isotope values；
Obtain the CO that tree branches breathing to be measured is produced in target forest in preset time period2In δ13C isotope values and
The CO that soil respiration to be measured is produced in target forest2In δ13C isotope values；
The CO produced is breathed according to the tree branches to be measured of acquisition2In δ13C isotope values and soil respiration to be measured are produced
CO2In δ13C isotope values, determine the proportionate relationship of each breathing component in the forest ecosystem；
Obtain the Carbon flux of the soil to be measured；
The Carbon flux of the soil to be measured of acquisition is substituted into the proportionate relationship of each breathing component in the forest ecosystem,
Obtain forest ecosystem breathing Carbon flux.
Alternatively, the Atmospheric CO for obtaining different height in target forest layer in preset time period2Concentration and air
CO2In δ13C isotope values, including：
Using carbon isotope analyser coordinate Port Multiplier, in preset time period target forest layer in different height it is big
Gas CO2Concentration and Atmospheric CO2In δ13C isotope values carry out layering measure；
Wherein, the carbon isotope analyser is connected by the Port Multiplier with multiple air sampling probes, described big
Gas sampling probe is set in advance in different height in forest to be measured layer, the air sampling probe by gas collection pipeline with
The Port Multiplier is connected.
Alternatively, Port Multiplier is coordinated to carry out layering survey to different height in target forest layer using carbon isotope analyser
The fixed time includes：Buffer stabilization time and practical measurement time；
The buffering stabilization time, the practical measurement time, the buffering stabilization time was less than the reality rear preceding
Border minute.
Alternatively, it is described to obtain the CO that tree branches breathing to be measured is produced in target forest in preset time period2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C isotope values, including：
Coordinate Port Multiplier using carbon isotope analyser, in preset time period, respectively to being treated in target forest
The branch air chamber and the soil air chamber on soil to be measured in target forest surveyed in tree branches are measured, and obtain pre-
If the CO that tree branches breathing to be measured is produced in target forest in the period2In δ13It is to be measured in C isotope values and target forest
The CO that soil respiration is produced2In δ13C isotope values；
Wherein, the carbon isotope analyser by the Port Multiplier respectively with the branch air chamber and the SOIL GAS
Room is connected, and the branch air chamber and the soil air chamber are connected by gas collection pipeline with the Port Multiplier.
Alternatively, using carbon isotope analyser coordinate Port Multiplier branch air chamber and soil air chamber are measured when
Between include：Buffer stabilization time and practical measurement time；
The buffering stabilization time, the practical measurement time, the buffering stabilization time was less than the reality rear preceding
Border minute.
Alternatively, in the target forest layer according to acquisition different height Atmospheric CO2Concentration and Atmospheric CO2In
δ13C isotope values, obtain forest ecosystem breathing δ13C isotope values, including：
According to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13C isotopes
Value, using keeling curve methods, draws Atmospheric CO2In δ13C isotope values and Atmospheric CO2Concentration scatterplot reciprocal
Figure；
Using linear equation y=ax+b, the scatter diagram is fitted, forest ecosystem breathing δ is obtained13The same positions of C
Plain value δ13Ceco；
Wherein, y is δ13C isotope values, x is Atmospheric CO2Concentration inverse, b be forest ecosystem breathe δ13C is same
The plain value δ in position13Ceco, a is equation parameter.
Alternatively, the CO that tree branches breathing to be measured is produced in target forest in the preset time period2In δ13C is same
The CO that soil respiration to be measured is produced in position element value and target forest2In δ13C isotope values are by repeatedly measuring averaging
What value was obtained.
Alternatively, it is described that the CO produced is breathed according to the tree branches to be measured of acquisition2In δ13C isotope values and to be measured
The CO that soil respiration is produced2In δ13C isotope values, determine the proportionate relationship of each breathing component in the forest ecosystem,
Including：
The CO produced is breathed according to the tree branches to be measured of acquisition2In δ13C isotope values and soil respiration to be measured are produced
CO2In δ13C isotope values, by isotope conservation principle and the principle of mass conservation, obtain and are respectively exhaled in forest ecosystem
Inhale the proportionate relationship of component；
Wherein, the isotope conservation principle is：
δ13Ceco×Reco=δ13Ctree×Rtree+δ13Csoil×Rsoil,
RecoCarbon flux, δ are breathed for forest ecosystem13Cecoδ is breathed for forest ecosystem13C isotope values, Rtree
Carbon flux, δ are breathed for tree branches to be measured13CtreeThe CO produced is breathed for tree branches to be measured2In δ13C isotope values,
RsoilFor soil respiration Carbon flux to be measured, δ13CsoilThe CO produced for soil respiration to be measured2In δ13C isotope values；
The principle of mass conservation is：
Reco=Rtree+Rsoil；
The proportionate relationship f of each breathing component in the forest ecosystem of acquisitionsoilFor：
Alternatively, the Carbon flux for obtaining the soil to be measured, including：
Using the soil carbon flux measuring system being connected with the soil air chamber, the carbon for obtaining the soil to be measured leads to
Amount.
As shown from the above technical solution, forest ecosystem of the invention breathing Carbon flux assay method, pre- by obtaining
If in the period target forest layer in different height Atmospheric CO2Concentration and Atmospheric CO2In δ13C isotope values, and according to
It obtains forest ecosystem breathing δ13C isotope values, obtain tree branches breathing to be measured in target forest in preset time period
The CO of generation2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C isotope values, and
According to the proportionate relationship of each breathing component in its determination forest ecosystem, the Carbon flux of soil to be measured is obtained, by treating for acquisition
The Carbon flux for surveying soil substitutes into the proportionate relationship of each breathing component in forest ecosystem, obtains forest ecosystem breathing carbon
Flux, thus, can not only overcome case method is short, can not carry out Continuous Observation to forest ecosystem breathing Carbon flux to lack
Point, additionally it is possible to overcome microclimate method to may not apply to the restrictive condition of complicated landform, can give birth in the forest of MODEL OVER COMPLEX TOPOGRAPHY
The Carbon flux more accurately determined in state system between air and plant changes, and technology is provided for Global Scale carbon cycle process
Support.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will to embodiment or
The accompanying drawing used required in description of the prior art is briefly described, it should be apparent that, drawings in the following description are this hairs
Some bright embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
A kind of flow for forest ecosystem breathing Carbon flux assay method that Fig. 1 provides for one embodiment of the invention is shown
It is intended to；
Fig. 2 breathes the device that Carbon flux assay method is utilized by forest ecosystem provided in an embodiment of the present invention
Structural representation.
In figure, 1 is air sampling probe, and 2 be branch air chamber, and 3 be soil air chamber, and 4 be soil carbon flux measuring system, 5
It is carbon isotope analyser for Port Multiplier, 6,7 be bioassay standard wood.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, clear, complete description is carried out to the technical scheme in the embodiment of the present invention, it is clear that described embodiment is only
Only it is a part of embodiment of the invention, rather than whole embodiments.Based on embodiments of the invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
Fig. 1 shows that the flow for the forest ecosystem breathing Carbon flux assay method that one embodiment of the invention is provided is shown
It is intended to, as shown in figure 1, the forest ecosystem breathing Carbon flux assay method of the present embodiment is as described below.
101st, the Atmospheric CO of different height in target forest layer in preset time period is obtained2Concentration and Atmospheric CO2In
δ13C isotope values.
In a particular application, the step 101 can utilize the carbon isotope analyser 6 in Fig. 2 to coordinate Port Multiplier 5, right
In preset time period target forest layer in different height Atmospheric CO2Concentration and Atmospheric CO2In δ13C isotope values are carried out
Layering is determined；
Wherein, the carbon isotope analyser 6 is connected by the Port Multiplier 5 with multiple air sampling probes 1, institute
State air sampling probe 1 and be set in advance in different height in forest layer to be measured, the air sampling probe 1 passes through gas collection
Pipeline is connected with the Port Multiplier 5.
In a particular application, the carbon isotope analyser 6 can be LGR carbon isotope analysers.
Specifically, the time that different height in target forest layer be layered measure can be included：When buffering is stable
Between and the practical measurement time；
The buffering stabilization time, the practical measurement time, the buffering stabilization time was less than the reality rear preceding
Border minute.For example, the time to every layer of measure of different height in target forest layer can be 5 minutes, wherein, first 2 minutes
It it is within latter 3 minutes the practical measurement time to buffer stabilization time.
102nd, according to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13C is same
Position element value, obtains forest ecosystem breathing δ13C isotope values.
In a particular application, the step 102 can be specifically included：
According to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13C isotopes
Value, using keeling curve methods, draws Atmospheric CO2In δ13C isotope values and Atmospheric CO2Concentration scatter diagram reciprocal
(e.g., can be drawn in excel softwares)；
Using linear equation y=ax+b, the scatter diagram is fitted, forest ecosystem breathing δ is obtained13The same positions of C
Plain value δ13Ceco；
Wherein, y is δ13C isotope values, x is Atmospheric CO2Concentration inverse, b be forest ecosystem breathe δ13C is same
The plain value δ in position13Ceco, a is equation parameter.
103rd, trees (i.e. bioassay standard wood 7) branch breathing generation to be measured in target forest in preset time period is obtained
CO2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C isotope values.
In a particular application, the step 103 can utilize the carbon isotope analyser 6 in Fig. 2 to coordinate Port Multiplier 5,
In preset time period, respectively to the branch air chamber 2 in target forest in tree branches to be measured and installed in target forest
In soil air chamber 3 on soil to be measured be measured, obtain tree branches breathing production to be measured in target forest in preset time period
Raw CO2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C isotope values；
Wherein, the carbon isotope analyser 6 by the Port Multiplier 5 respectively with the branch air chamber 2 and the soil
Air chamber 3 is connected, and the branch air chamber 2 and the soil air chamber 3 are connected by gas collection pipeline with the Port Multiplier 5
Connect.
Specifically, the time that branch air chamber 2 and soil air chamber 3 are measured can be included：Buffer stabilization time and
The practical measurement time；
The buffering stabilization time, the practical measurement time, the buffering stabilization time was less than the reality rear preceding
Border minute.For example, the time being measured to branch air chamber 2 and soil air chamber 3 can be 5 minutes, wherein, first 2 points
Clock is buffering stabilization time, is within latter 3 minutes the practical measurement time.
Further, in order to obtain in more accurately measurement result, the preset time period trees to be measured in target forest
The CO that branch breathing is produced2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13The same positions of C
Plain value can be by repeatedly measuring acquisition of averaging.
104th, the CO produced is breathed according to the tree branches to be measured of acquisition2In δ13C isotope values and soil respiration to be measured
The CO of generation2In δ13C isotope values, determine in the forest ecosystem each breathing component (including：Soil respiration and plant
Thing breathe) proportionate relationship.
In a particular application, the step 104 can be specifically included：
The CO produced is breathed according to the tree branches to be measured of acquisition2In δ13C isotope values and soil respiration to be measured are produced
CO2In δ13C isotope values, by isotope conservation principle and the principle of mass conservation, obtain and are respectively exhaled in forest ecosystem
Inhale the proportionate relationship f of componentsoil；
Wherein, the isotope conservation principle is：
δ13Ceco×Reco=δ13Ctree×Rtree+δ13Csoil×Rsoil (1)
RecoCarbon flux, δ are breathed for forest ecosystem13Cecoδ is breathed for forest ecosystem13C isotope values, Rtree
Carbon flux, δ are breathed for tree branches to be measured13CtreeThe CO produced is breathed for tree branches to be measured2In δ13C isotope values,
RsoilFor soil respiration Carbon flux to be measured, δ13CsoilThe CO produced for soil respiration to be measured2In δ13C isotope values；
The principle of mass conservation is：
Reco=Rtree+Rsoil (2)
The proportionate relationship f of each breathing component in the forest ecosystem of acquisitionsoilFor：
Wherein, the unit of each component breathing Carbon flux is μm olm in forest ecosystem-2·s-1, isotope abundance value
Unit be ‰.
105th, the Carbon flux of the soil to be measured is obtained.
In a particular application, the step 105 can utilize the soil carbon being connected with the soil air chamber 3 in Fig. 2
Flux measurement system 4, obtains the Carbon flux of the soil to be measured.
For example, the soil carbon flux measuring system 4 can be LI-8100 soil carbon flux measuring systems.
106th, the ratio that the Carbon flux of the soil to be measured of acquisition is substituted into each breathing component in the forest ecosystem is closed
System, obtains forest ecosystem breathing Carbon flux.
It is understood that according to the proportionate relationship of each breathing component in the forest ecosystem：Understand,AndThe carbon of soil to be measured leads to
Measure RsoilObtain in step 105, forest ecosystem breathing Carbon flux R can be obtained by calculatingeco。
The forest ecosystem breathing Carbon flux assay method of the present embodiment, it is gloomy by obtaining target in preset time period
The Atmospheric CO of different height in woods layer2Concentration and Atmospheric CO2In δ13C isotope values, and Forest ecosystem is obtained according to it
System breathing δ13C isotope values, obtain the CO that tree branches breathing to be measured is produced in target forest in preset time period2In δ13C
The CO that soil respiration to be measured is produced in isotope value and target forest2In δ13C isotope values, and given birth to according to its determination forest
The proportionate relationship of each breathing component, obtains the Carbon flux of soil to be measured, by the Carbon flux generation of the soil to be measured of acquisition in state system
Enter the proportionate relationship of each breathing component in forest ecosystem, obtain forest ecosystem breathing Carbon flux, that is, use and stablize same
Each component during the plain technology in position is breathed to forest ecosystem (including：Soil respiration and plant respiration) split and determine it
Proportionate relationship, is then derived by determining soil respiration Carbon flux and obtains forest ecosystem breathing Carbon flux, thus, no
Can only overcome case method it is short, can not to forest ecosystem breathe Carbon flux carry out Continuous Observation shortcoming, additionally it is possible to overcome
Microclimate method may not apply to the restrictive condition of complicated landform, can be in the forest ecosystem of MODEL OVER COMPLEX TOPOGRAPHY more
The Carbon flux accurately determined between air and plant changes, and technical support is provided for Global Scale carbon cycle process.
In order to preferably illustrate, Capital Circle ecological observation station in Haidian District, Beijing City is located at enforcement place below,
Exemplified by April 1st, 2016 was measured to April 28, the forest ecosystem breathing Carbon flux assay method of the present embodiment can
With including：
First, build air sampling probe 1 in forest as shown in Figure 2, be laid in respectively 0.05m in forest, 2m, 5m, 8m,
12m and 18m height, and branch air chamber and soil air chamber are installed, gas collection pipeline is connected to carbon isotope via Port Multiplier 5
Analyzer 6, determines the Atmospheric CO of different height in forest layer2Concentration and Atmospheric CO2In δ13C isotope values, and
The CO that tree branches breathing to be measured is produced in forest2In δ13The CO that soil respiration to be measured is produced in C isotope values and forest2
In δ13C isotope values, and laying LI-8100 soil carbon fluxes measuring system 4 is installed in forest, determine soil to be measured
Carbon flux.
Specifically, the carbon isotope analyser 6 can carry out instrument calibration with multi-point calibration method, and Port Multiplier 5 is each
Passage is accessed 5 minutes, wherein, it is within first 2 minutes buffering stabilization time, is within latter 3 minutes the practical measurement time.
2nd, according to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13The same positions of C
Element value, using keeling curve methods, Atmospheric CO is drawn in excel softwares2In δ13C isotope values and Atmospheric CO2It is dense
The scatter diagram reciprocal of degree；Using linear equation y=ax+b, the scatter diagram is fitted, forest ecosystem is obtained and exhales
Inhale δ13C isotope values δ13Ceco；Wherein, y is δ13C isotope values, x is Atmospheric CO2Concentration inverse, b is forest ecology
System breathes δ13C isotope values δ13Ceco, a is equation parameter.During numerical fitting, if Atmospheric CO2Change in concentration is smaller,
Then fitting effect is poor, therefore in the present embodiment, what is gained atmosphere data carried out in once fitting, linear equation within every 7 days cuts
It is forest ecosystem breathing δ away from b13C isotope values δ13Ceco；The CO produced is breathed for tree branches to be measured2In
δ13C isotope values δ13CtreeThe CO produced with soil respiration to be measured2In δ13C isotope values δ13Csoil, carry out once within every 7 days
Average value processing.The simulation of the present embodiment concrete numerical value is as shown in table 1 below with observed result.
Table 1
| Time | δ13Ctree | δ13Csoil | δ13Ceco |
| 4.1-4.7 | -27.65 | -24.35 | -26.45 |
| 4.8-4.14 | -29.12 | -25.35 | -27.66 |
| 4.15-4.21 | -28.11 | -25.67 | -26.98 |
| 4.22-4.28 | -28.95 | -26.13 | -27.15 |
3rd, the CO produced is breathed according to the tree branches to be measured of acquisition2In δ13C isotope values and soil respiration to be measured
The CO of generation2In δ13C isotope values, by isotope conservation principle and the principle of mass conservation, are obtained in forest ecosystem
The proportionate relationship f of each breathing componentsoil；
Wherein, the isotope conservation principle is：
δ13Ceco×Reco=δ13Ctree×Rtree+δ13Csoil×Rsoil (1)
RecoCarbon flux, δ are breathed for forest ecosystem13Cecoδ is breathed for forest ecosystem13C isotope values, Rtree
Carbon flux, δ are breathed for tree branches to be measured13CtreeThe CO produced is breathed for tree branches to be measured2In δ13C isotope values,
RsoilFor soil respiration Carbon flux to be measured, δ13CsoilThe CO produced for soil respiration to be measured2In δ13C isotope values；
The principle of mass conservation is：
Reco=Rtree+Rsoil (2)
The proportionate relationship f of each breathing component in the forest ecosystem of acquisitionsoilFor：
Wherein, the unit of each component breathing Carbon flux is μm olm in forest ecosystem-2·s-1, isotope abundance
The unit of value is ‰.
Data in the table 1 determined by April 1 to April 28, this step can obtain April 1 to the date in April 28
Between, the f of every 7 dayssoilRespectively：0.467 (1-7 days April), 0.493 (8-14 days April), 0.504 (15-21 days April) and
0.461 (22-28 days April).
4th, using the LI-8100 soil carbon fluxes measuring system 4 being connected with the soil air chamber 3, treated described in acquisition
The Carbon flux of soil is surveyed, during obtaining April 1 to April 28, the Carbon flux of every soil to be measured on the 7th is respectively：5.62g·m-2、5.87g·m-2、6.29g·m-2And 6.61gm-2。
5th, according to formulaUnderstand forest ecosystem breathing Carbon fluxApril 1 can be obtained
During day to 28 days, the forest ecosystem breathing Carbon flux R of every 7 daysecoRespectively 12.04gm-2、11.90g·m-2、
12.47g·m-2And 14.34gm-2。
The present embodiment forest ecosystem breathing Carbon flux assay method, can not only overcome case method it is short, can not be right
Forest ecosystem breathing Carbon flux carries out the shortcoming of Continuous Observation, additionally it is possible to overcome microclimate method to may not apply to intricately
The restrictive condition of shape, can more accurately be determined between air and plant in the forest ecosystem of MODEL OVER COMPLEX TOPOGRAPHY
Carbon flux changes, and technical support is provided for Global Scale carbon cycle process.
One of ordinary skill in the art will appreciate that：Realizing all or part of step of above method embodiment can lead to
The related hardware of programmed instruction is crossed to complete.Foregoing program can be stored in a computer read/write memory medium.The journey
Sequence upon execution, performs the step of including above-mentioned each method embodiment；And foregoing storage medium includes：ROM, RAM, magnetic disc
Or CD etc. is various can be with the medium of store program codes.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；
Although the present invention is described in detail with reference to foregoing embodiments, it will be understood by those within the art that：Its
The technical scheme described in foregoing embodiments can still be modified, or it is special to which part or whole technologies
Levy carry out equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from the right of the present invention
Claimed scope.
Claims (9)
1. a kind of forest ecosystem breathes Carbon flux assay method, it is characterised in that including：
Obtain the Atmospheric CO of different height in target forest layer in preset time period2Concentration and Atmospheric CO2In δ13C isotopes
Value；
According to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13C isotope values, are obtained
Forest ecosystem breathes δ13C isotope values；
Obtain the CO that tree branches breathing to be measured is produced in target forest in preset time period2In δ13C isotope values and target
The CO that soil respiration to be measured is produced in forest2In δ13C isotope values；
The CO produced is breathed according to the tree branches to be measured of acquisition2In δ13The CO that C isotope values and soil respiration to be measured are produced2
In δ13C isotope values, determine the proportionate relationship of each breathing component in the forest ecosystem；
Obtain the Carbon flux of the soil to be measured；
The Carbon flux of the soil to be measured of acquisition is substituted into the proportionate relationship of each breathing component in the forest ecosystem, obtained gloomy
Woods ecosystem respiration Carbon flux.
2. according to the method described in claim 1, it is characterised in that different in target forest layer in the acquisition preset time period
The Atmospheric CO of height2Concentration and Atmospheric CO2In δ13C isotope values, including：
Coordinate Port Multiplier using carbon isotope analyser, to the Atmospheric CO of different height in target forest layer in preset time period2's
Concentration and Atmospheric CO2In δ13C isotope values carry out layering measure；
Wherein, the carbon isotope analyser is connected by the Port Multiplier with multiple air sampling probes, and the air takes
Sample probe is set in advance in different height in forest to be measured layer, the air sampling probe by gas collection pipeline with it is described many
Road device is connected.
3. method according to claim 2, it is characterised in that coordinate Port Multiplier gloomy to target using carbon isotope analyser
The time of different height progress layering measure includes in woods layer：Buffer stabilization time and practical measurement time；
The buffering stabilization time, the practical measurement time, the buffering stabilization time was less than described actual survey rear preceding
Fix time.
4. according to the method described in claim 1, it is characterised in that treat assize in target forest in the acquisition preset time period
The CO that the breathing of wooden branch bar is produced2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C is same
Position element value, including：
Coordinate Port Multiplier using carbon isotope analyser, in preset time period, respectively to treating assize in target forest
Branch air chamber on wooden branch bar and the soil air chamber on soil to be measured in target forest are measured, and obtain preset time
The CO that tree branches breathing to be measured is produced in target forest in section2In δ13Soil to be measured is exhaled in C isotope values and target forest
Inhale the CO produced2In δ13C isotope values；
Wherein, the carbon isotope analyser is connected with the branch air chamber and the soil air chamber respectively by the Port Multiplier
Connect, the branch air chamber and the soil air chamber are connected by gas collection pipeline with the Port Multiplier.
5. method according to claim 4, it is characterised in that coordinate Port Multiplier to branch gas using carbon isotope analyser
The time that room and soil air chamber are measured includes：Buffer stabilization time and practical measurement time；
The buffering stabilization time, the practical measurement time, the buffering stabilization time was less than described actual survey rear preceding
Fix time.
6. according to the method described in claim 1, it is characterised in that different height in the target forest layer according to acquisition
Atmospheric CO2Concentration and Atmospheric CO2In δ13C isotope values, obtain forest ecosystem breathing δ13C isotope values, including：
According to the Atmospheric CO of different height in the target forest of acquisition layer2Concentration and Atmospheric CO2In δ13C isotope values, are utilized
Keeling curve methods, draw Atmospheric CO2In δ13C isotope values and Atmospheric CO2Concentration scatter diagram reciprocal；
Using linear equation y=ax+b, the scatter diagram is fitted, forest ecosystem breathing δ is obtained13C isotope values
δ13Ceco；
Wherein, y is δ13C isotope values, x is Atmospheric CO2Concentration inverse, b be forest ecosystem breathe δ13C isotope values
δ13Ceco, a is equation parameter.
7. according to the method described in claim 1, it is characterised in that trees branch to be measured in target forest in the preset time period
The CO that bar breathing is produced2In δ13The CO that soil respiration to be measured is produced in C isotope values and target forest2In δ13C isotopes
Value is by repeatedly measuring acquisition of averaging.
8. according to the method described in claim 1, it is characterised in that described to breathe what is produced according to the tree branches to be measured of acquisition
CO2In δ13The CO that C isotope values and soil respiration to be measured are produced2In δ13C isotope values, determine the forest ecosystem
In each breathing component proportionate relationship, including：
The CO produced is breathed according to the tree branches to be measured of acquisition2In δ13The CO that C isotope values and soil respiration to be measured are produced2
In δ13C isotope values, by isotope conservation principle and the principle of mass conservation, obtain each breathing component in forest ecosystem
Proportionate relationship；
Wherein, the isotope conservation principle is：
δ13Ceco×Reco=δ13Ctree×Rtree+δ13Csoil×Rsoil,
RecoCarbon flux, δ are breathed for forest ecosystem13Cecoδ is breathed for forest ecosystem13C isotope values, RtreeTo be to be measured
Tree branches breathe Carbon flux, δ13CtreeThe CO produced is breathed for tree branches to be measured2In δ13C isotope values, RsoilTo treat
Survey soil respiration Carbon flux, δ13CsoilThe CO produced for soil respiration to be measured2In δ13C isotope values；
The principle of mass conservation is：
Reco=Rtree+Rsoil；
The proportionate relationship f of each breathing component in the forest ecosystem of acquisitionsoilFor：
9. method according to claim 4, it is characterised in that the Carbon flux of the acquisition soil to be measured, including：
Using the soil carbon flux measuring system being connected with the soil air chamber, the Carbon flux of the soil to be measured is obtained.
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