CN114544911A - Method and device for determining organic carbon delivery from plants to soil based on different ways - Google Patents
Method and device for determining organic carbon delivery from plants to soil based on different ways Download PDFInfo
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 230
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 230
- 239000002689 soil Substances 0.000 title claims abstract description 117
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Abstract
The invention provides a method and a device for determining organic carbon delivery to soil by plants in different ways, wherein the method comprises the following steps: measuring the initial volume weight of a filler in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column; burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; and inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to conveying organic carbon into the target soil through different ways.
Description
Technical Field
The invention belongs to the technical field of soil component detection, and particularly relates to a method and a device for determining organic carbon delivery to soil by plants based on different approaches.
Background
The organic carbon in the soil mainly comes from plants, and the degradation of litter and the secretion and turnover of root systems are two important ways for the plants to convey the organic carbon to the soil.
Due to the complexity of plant-soil interactions, the prior art lacks a uniform quantification of the contribution of plant-to-soil organic carbon and the contribution of the litter and root system pathways to organic carbon delivery, such as: in the prior art, the main accounting content of the carbon sink amount of the forest ecological system is the forest accumulation amount, and the accounting for the soil organic carbon increase amount is lost; although the aboveground biomass of the grassland ecosystem is mainly converted into forage grass to release carbon, the organic carbon reserves of soil increased by artificial intervention measures such as no-tillage reseeding and seasonal fallow aiming at deteriorated grasslands are not clear, so that the carbon sink effect of the grassland ecological quality and productivity improvement technology cannot be evaluated, and the carbon sink accounting process of the land ecosystem is unreliable.
Disclosure of Invention
The invention provides a method and a device for determining organic carbon delivered to soil by plants in different ways, which are used for solving the defect that the carbon sink accounting process is incomplete and incomplete due to the fact that a uniform quantification method is not available when organic carbon delivered to soil by plants is determined in the prior art, and the reliability of the carbon sink accounting process of a land ecosystem is improved.
The invention provides a method for determining organic carbon delivery to soil by plants based on different ways, which comprises the following steps:
measuring the initial volume weight of a filler in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column; burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; and inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to conveying organic carbon into the target soil through different ways.
According to the invention, the method for determining the organic carbon delivery to the soil by the plants based on different routes further comprises the following steps:
respectively measuring a first organic carbon content and a first carbon stable isotope ratio of fillers in a root system growth column of the target monitoring column, and respectively measuring a second organic carbon content and a second carbon stable isotope ratio of fillers in a litter degradation column of the target monitoring column and a third carbon stable isotope ratio of fillers in a contrast column of the target monitoring column; and respectively measuring the fourth carbon stable isotope ratio of the thin root in the root system growth column of the target monitoring column and the fifth carbon stable isotope ratio of the litter on the litter degrading column of the target monitoring column.
Setting the side wall and the upper and lower bottom surfaces of the root system growth column as plastic gauze with the aperture of 2mm, respectively setting the side walls of the litter degradation column and the contrast column as PVC pipes, and setting the lower bottom surfaces of the litter degradation column and the contrast column as compact nets with the aperture of 1 mu m; simultaneously placing the arranged root growth column, the litter degradation column and the contrast column in corresponding pot holes for burying, and respectively arranging isolation covers with the aperture of 5mm above the root growth column and the contrast column; and removing the litter on the isolation cover according to a preset fixed period, and taking out the monitoring column after reaching a preset growth period so as to obtain the target monitoring column.
Inputting the target carbon stable isotope ratio into the model, and obtaining a target proportion according to the following formula:
Frt＝(δ13Cingrowth-δ13Ccontrol)/(δ13Crt-δ13Ccontrol)
Flitter＝(δ13Cdegradation-δ13Ccontrol)/(δ13Clitter-δ13Ccontrol)
wherein, FrtAnd FlitterRespectively representing the proportion of soil carbon from root systems and withered and fallen objects; delta13Cingrowth、δ13CdegradationAnd delta13CcontrolRespectively representing the carbon isotope ratios of fillers in the root growth column, the litter degrading column and the contrast column; delta13CrtAnd delta13ClitterRespectively representing the carbon isotope ratios of the root system in the root system growing column and the litter on the litter degrading column; inputting the target proportion, the target organic carbon content, the height of the target monitoring column and the initial volume weight into the model, and obtaining the net contribution of the plant to conveying organic carbon into the target soil through different ways according to the following formula:
Crd-net＝ρ×[C]ingrowth×Frt×h×10000
Cld-net＝ρ×[C]degradation×Flitter×h×10000
wherein, Crd-netAnd Cld-netRespectively representing the net contribution amount of root system input and litter degradation to soil carbon, wherein rho is the initial volume weight of the filler in the monitoring column, [ C]ingrowthAnd [ C]degratationRespectively representing the organic carbon content of the fillers in the root growth column and the litter degradation column during sampling, h is the height of the monitoring column, and 10000 is a unit conversion coefficient from the organic carbon content to the net contribution amount.
And determining the content of the target organic carbon by using an element analyzer, determining the initial volume weight by using a cutting ring method, and determining the ratio of the target carbon stable isotope by using a carbon stable isotope analyzer.
The height of the target monitoring column is within 30cm-50 cm.
The filler is a mixture of sand and soil, wherein the volume ratio of the sand to the soil is 1: 1.
The invention also provides a device for determining organic carbon delivery to soil by plants based on different routes, which comprises:
the device comprises a first determination module, a second determination module and a third determination module, wherein the first determination module is used for determining the initial volume weight of a filler in a preset monitoring column, and the monitoring column comprises a root system growth column, a litter degradation column and a control column; the second determination module is used for burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain the target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; and the calculation module is used for inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to the organic carbon conveyed into the target soil through different ways.
The invention also provides an electronic device, which comprises a memory, a processor and a computer program stored on the memory and capable of running on the processor, wherein the processor executes the program to realize the method for measuring the organic carbon delivery to the soil based on the plants in different ways.
The present invention also provides a non-transitory computer readable storage medium having stored thereon a computer program which, when executed by a processor, implements a method for determining organic carbon delivery to soil based on different pathways of plants as described in any of the above.
The invention provides a method and a device for determining organic carbon delivery to soil by plants in different ways, which comprises the steps of firstly determining the initial volume weight of a filler in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column, then burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column, and finally inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plants to the organic carbon delivery to the target soil by different ways; the invention provides a method for quantitatively monitoring organic carbon delivered to soil by plants, which improves the reliability of carbon sink amount accounting of a land ecosystem.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.
FIG. 1 is a schematic flow chart of an assay method for delivering organic carbon to soil by plants in different ways according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a set of monitoring columns according to another embodiment of the present invention;
FIG. 3 is a schematic diagram of an apparatus for determining organic carbon delivery to soil by plants according to different routes, provided by an embodiment of the present invention;
fig. 4 is a schematic structural diagram of an electronic device provided in the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The following describes, with reference to fig. 1, an assay method for organic carbon delivery to soil by plants based on different pathways, provided by an embodiment of the present invention, including:
Due to the fact thatThe organic carbon in the soil mainly comes from two important ways, namely, the degradation of the plant litter and the secretion and turnover of the root system, therefore, a group of monitoring columns which are composed of root system growing columns, litter degrading columns and control columns and used for quantifying the organic carbon conveyed to the soil by the plant is arranged in the embodiment, wherein the root system growing columns are used for monitoring the change condition of the organic carbon conveyed to the soil by the plant in a way of the secretion and turnover of the root system, and can be combined with the change condition of the organic carbon filled in the control columns to calculate the contribution amount of the organic carbon conveyed to the soil by the plant in a way of the secretion and turnover of the root system; the litter degradation column is used for monitoring the change condition of organic carbon transported to soil by the plant in the way of litter degradation, and can be combined with the change condition of organic carbon filled in the control column to calculate the contribution amount of the plant in the way of litter degradation to the soil; the filler in the control column is used as a control group in the monitoring column of the embodiment in a mode of no root secretion, turnover and litter degradation for conveying organic carbon to the filler; in this embodiment, before burying the monitoring pillars in the corresponding pits, an initial volume weight of a filler in a preset monitoring pillar is determined, specifically: filling the fillers of the root growth column, the litter degradation column and the control column into a cutting ring (volume of 100 cm)3) And weighing the weights of the front and rear ring cutters before filling the filler, wherein the corresponding weight is accurate to 0.01g, so as to obtain the initial volume weight of the filler in the monitoring column.
102, burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column.
Specifically, in this embodiment, before the growth cycle of the plant starts, the initial volume weight of the filler in the monitoring column is obtained first, then the root growth column, the litter degradation column and the control column are buried in different pot holes in the soil in the experimental area, after at least two growth cycles, the buried monitoring column is taken out for determining the change condition of the organic carbon contained in the filler in the taken-out monitoring column, specifically: and measuring the parameters such as the organic carbon content and the carbon stable isotope ratio of the filler in each monitoring column after being taken out, the carbon stable isotope ratio of the plant fine roots in the root growth column, the carbon stable isotope ratio of the litter on the litter degradation column and the like, thereby providing convenience for calculating the net contribution amount of the plants in the experimental area to the organic carbon conveyed by the soil in the subsequent process.
It should be noted that the growth cycle of different plants may be different, and the growth cycle of the plants adopted in this embodiment is one year.
103, inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to the organic carbon delivery in the target soil through different ways.
It can be understood that, in this embodiment, after obtaining parameters such as the organic carbon content and the carbon-stable isotope ratio of the filler in the root growth column after being taken out, the organic carbon content and the carbon-stable isotope ratio of the filler in the litter degradation column, the carbon-stable isotope ratio of the filler in the control column, the carbon-stable isotope ratio of the thin root in the root growth column, and the carbon-stable isotope ratio of the litter on the litter degradation column, the parameters are input to the isotope binary mixture model in combination with the height h of the monitoring column and the initial bulk weight ρ of the filler of the monitoring column, which are measured in advance, to calculate the net contribution amount of organic carbon transported to soil by the plant in the experimental area; it should be noted that the measurement heights h of the root growth column, the litter degradation column and the control column included in the monitoring column should be kept consistent, and the initial bulk densities ρ of the fillers of the root growth column, the litter degradation column and the control column should also be kept consistent.
The embodiment provides a method for quantitatively monitoring organic carbon delivered to soil by a plant, and the reliability of carbon sink amount accounting of a land ecosystem is improved.
Optionally, respectively measuring a first organic carbon content and a first carbon stable isotope ratio of fillers in the root growth column of the target monitoring column, and respectively measuring a second organic carbon content and a second carbon stable isotope ratio of fillers in the litter degradation column of the target monitoring column and a third carbon stable isotope ratio of fillers in a control column of the target monitoring column; and respectively measuring the fourth carbon stable isotope ratio of the thin root in the root system growth column of the target monitoring column and the fifth carbon stable isotope ratio of the litter on the litter degrading column of the target monitoring column.
It can be understood that, in this embodiment, the organic carbon content and the carbon-stable isotope ratio of the filler in the root system growth column after being taken out, the organic carbon content and the carbon-stable isotope ratio of the filler in the litter degradation column, the carbon-stable isotope ratio of the filler in the control column, the carbon-stable isotope ratio of the thin root in the root system growth column, and the carbon-stable isotope ratio of the litter on the litter degradation column are measured.
Specifically, in this embodiment, after each growth cycle of a plant in an experimental area is finished, a litter sample within a range of 0.5m × 0.5m near a monitoring column arrangement point is collected; after at least two growth cycles, simultaneously digging out a root growth column, a litter degradation column and a control column from the arrangement point, respectively collecting filler samples in the columns, and collecting root samples in the root growth column; for collected litter, soil animals in a sample are removed firstly, then floating soil is washed in deionized water to obtain a fresh litter sample, then the fresh litter sample is dried at 70 ℃ for 72 hours to obtain a dried litter sample, the dried litter sample is weighed, the weighing mass of the litter sample is accurate to 0.01g, and finally the litter amount is obtained; for a plant root system, in the embodiment, firstly, a 2mm sieve is used for separating fillers from the plant root system, then dead roots are removed, live roots are washed by deionized water to obtain a fresh root sample, then, the fresh root sample is dried for 72 hours at 70 ℃, a dry root sample is obtained and weighed, the weighing mass of the root system sample is accurate to 0.01g, and finally, the plant root system biomass is obtained; for the filler, in the embodiment, the root system is removed from the filler of the root system growth column, the litter is removed from the top of the litter degradation column, so as to obtain filler samples in the root system growth column, the litter degradation column and the control column, and then the filler samples are naturally air-dried, so as to obtain a dry filler sample; after obtaining the dried litter, dried root and dried filler samples, the present example measured these samples using an aid to obtain the desired organic carbon content and carbon stable isotope ratio.
The embodiment provides a method for extracting the target organic carbon content and the target carbon stable isotope ratio from the extracted monitoring column, and provides convenience for calculating the net contribution of organic carbon conveyed to soil by plants through different ways in the subsequent process.
Optionally, the side wall and the upper and lower bottom surfaces of the root growth column are both provided with plastic gauze with the aperture of 2mm, the side walls of the litter degradation column and the contrast column are respectively provided with PVC pipes, and the lower bottom surfaces of the litter degradation column and the contrast column are provided with dense nets with the aperture of 1 μm; simultaneously placing the arranged root growth column, the litter degradation column and the contrast column in corresponding pot holes for burying, and respectively arranging isolation covers with the aperture of 5mm above the root growth column and the contrast column; and removing the litter on the isolation cover according to a preset fixed period, and taking out the monitoring column after reaching a preset growth period so as to obtain the target monitoring column.
Specifically, each group of monitoring pillars has a structure as shown in fig. 2, in this embodiment, a root growth pillar 201, a litter degradation pillar 202, and a control pillar 203 are embedded in three preset pot holes side by side in an experimental area, wherein the three pillars are in seamless contact with the side wall and the bottom of the corresponding pot hole, the distance between adjacent pot holes is within a range of 10cm to 20cm, the distance between adjacent pot holes is twice the diameter of the bottom surface of the monitoring pillar, that is, the diameter of the bottom surface of the three pillars is within a range of 5cm to 10 cm; then, the side wall and the upper and lower bottom surfaces of the root system growth column 201 are provided with plastic gauze with the aperture of 2mm, so that the internal filler is not scattered, and the fine roots of the plant can enter the column to grow, the root system growth column is placed in the pot hole, the top of the root system growth column is covered with a separation cover with the aperture of 5mm, the withered falling objects on the separation cover are removed in a fixed period of every week or every month, so as to prevent the contact between the withered falling objects and the filler in the column, the side wall of the withered falling object degradation column 202 is provided with a PVC pipe, the lower bottom surface of the column is provided with a compact net with the aperture of 1 mu m, so that the plant root system and soil fungi can not enter the column, and simultaneously the hydrothermal exchange in the vertical direction is not blocked, the withered falling object degradation column 202 is buried in the pot hole, the top of the column is not provided with a shield, the withered falling objects are in contact with the filler, and naturally accumulated and degraded, the side wall of the control column 203 is provided with the PVC pipe, and the lower bottom surface is provided with the compact net with the aperture of 1 mu m, after the control column 203 is buried in the pit, a separation cover with the aperture of 5mm is covered on the top of the control column, and the litter on the separation cover is removed at a fixed period of every week or every month; finally, after two growth cycles (two years) are carried out, the target monitoring columns, namely the taken-out root growth column 201, the litter degrading column 202 and the control column 203 are taken out from the experimental area, at this time, organic carbon conveyed to the column filler from the plant root system of the experimental area exists in the root growth column 201, organic carbon conveyed to the column filler from the litter degrading column 202 of the plant of the experimental area exists in the litter degrading column, and the control column 203 serves as a control group of the experiment.
The embodiment provides a method for burying a monitoring column after differential setting and obtaining a target monitoring column, which adopts a control variable method and sets a control group, and provides convenience for obtaining a dried litter sample, a dried root sample and a dried filler sample from the target monitoring column in the subsequent process so as to calculate the net contribution amount of organic carbon transported to soil by a plant through different ways.
Optionally, the target carbon stable isotope ratio is input into the model, and a target ratio is obtained according to the following formula:
Frt＝(δ13Cingrowth-δ13Ccontrol)/(δ13Crt-δ13Ccontrol)
Flitter＝(δ13Cdegradation-δ13Ccontrol)/(δ13Clitter-δ13Ccontrol)
wherein, FrtAnd FlitterRespectively representing the proportion of soil carbon from root systems and withered and fallen objects; delta13Cingrowth、δ13CdegradationAnd delta13CcontrolRespectively representing the carbon isotope ratios of fillers in the root growth column, the litter degradation column and the control column；δ13CrtAnd delta13ClitterRespectively representing the carbon isotope ratios of the root system in the root system growing column and the litter on the litter degrading column; inputting the target proportion, the target organic carbon content, the height of the target monitoring column and the initial volume weight into the model, and obtaining the net contribution of organic carbon in the target soil according to the following formula:
Crd-net＝ρ×[C]ingrowth×Frt×h×10000
Cld-net＝ρ×[C]degradation×Flitter×h×10000
wherein, Crd-netAnd Cld-netRespectively representing the net contribution amount of root system input and litter degradation to soil carbon, wherein rho is the initial volume weight of the filler in the monitoring column, [ C]ingrowthAnd [ C]degratationRespectively representing the organic carbon content of the fillers in the root growth column and the litter degradation column during sampling, h is the height of the monitoring column, and 10000 is a unit conversion coefficient from the organic carbon content to the net contribution amount.
Specifically, in this embodiment, the carbon-stable isotope ratio δ of the filler in the root growth column is set13CingrowthComparison of the carbon-stable isotope ratio delta of the filling in the column13CcontrolCarbon stable isotope ratio delta of plant root system in root system growth column13CrtSubstituting into the following equation:
Frt＝(δ13Cingrowth-δ13Ccontrol)/(δ13Crt-δ13Ccontrol)
calculating the ratio F of the carbon source of the soil from the root systemrtI.e. the proportion of organic carbon which the plant delivers to the soil through the root system, and FrtInitial volume weight rho of filler, organic carbon content [ C ] of filler in root growth column during sampling]ingrowthAnd substituting the height h of the monitoring column into the following formula:
Crd-net＝ρ×[C]ingrowth×Frt×h×10000
the net contribution C of the root system input to the soil carbon in this example is obtainedrd-netI.e. the net contribution of the plant root system to the input of organic carbon into the soil; meanwhile, in the embodiment, the carbon isotope ratio delta of the filler in the litter degradation column13CdegradationComparison of the carbon-stable isotope ratio delta of the filling in the column13CcontrolAnd the carbon isotope ratio delta of the litter on the litter degrading column13ClitterSubstituting into the following equation:
Flitter＝(δ13Cdegradation-δ13Ccontrol)/(δ13Clitter-δ13Ccontrol)
calculating the proportion F of the carbon source of the soil from the litterlitterI.e. the proportion of organic carbon which the plant delivers to the soil by degradation of the litter, and FlitterInitial volume weight rho of filler, organic carbon content [ C ] of filler in litter degradation column during sampling]degratationAnd substituting the height h of the monitoring column into the following formula:
Cld-net＝ρ×[C]degradation×Flitter×h×10000
the net contribution C of the degradation of the litter in this example to the carbon in the soil was obtainedld-netThat is, the net contribution of the plant litter to the soil carbon input, in this example, the unit of the net contribution of the plant root input and the litter degradation to the soil carbon is g C m-2The unit of initial volume weight is g.m-3The unit of the organic carbon content is percent (%), and the unit of the monitoring column height is cm; it should be noted that the target monitoring columns in this embodiment include root growth columns, litter degradation columns, and control columns that have the same measurement height.
The embodiment provides a method for acquiring net contribution of a plant to conveying organic carbon to soil based on a carbon stable isotope ratio and organic carbon content, which can effectively quantify the contribution degree of a litter and a root system to the increase of soil organic carbon storage of the plant.
Optionally, the content of the target organic carbon is determined by using an element analyzer, the initial volume weight is determined by using a cutting ring method, and the ratio of the target carbon stable isotope is determined by using a carbon stable isotope analyzer.
It is understood that, in order to extract the required organic carbon content and carbon stable isotope ratio from the obtained dried litter sample, dried root sample and dried filler sample, the dried litter sample, dried root sample and dried filler sample are first crushed by a ball mill, then the obtained carbon content and nitrogen content are measured by an elemental analyzer, and the obtained carbon stable isotope ratio (δ) is measured by a stable isotope analyzer13C) (ii) a In addition, in the embodiment, the initial volume weight of the filler in the monitoring column before burying is measured and obtained by adopting a cutting ring method.
The method provided by the embodiment provides a method for acquiring the organic carbon content, the carbon stable isotope ratio and the initial volume weight, and provides convenience for calculating the net contribution of the plant to the organic carbon delivery to the soil through different ways in the subsequent process.
Optionally, the height of the target monitoring column is within 30cm-50 cm.
It can be understood that the soil quality, plant species and vegetation coverage of different experimental areas are different, therefore, in this embodiment, the root growth column, the litter degradation column and the control column included in the monitoring column are set to have the same size and height of the bottom surface, the height of the bottom surface is in the range of 30cm to 50cm, the diameter of the bottom surface is in the range of 5cm to 10cm, and the specific height is flexibly selected according to different experimental areas; in addition, the embodiment can determine the organic carbon delivered to the soil by the plants under the situations of forests, grasslands, farmlands, garden greenbelts and the like, for example, one application scenario is as follows: randomly selecting 3 samples of 1m multiplied by 1m from a zero-tillage zero-planting grassland and a contrast grassland (grassland without zero-tillage zero-planting measures), wherein the number of the samples is 6, arranging 2 groups of monitoring columns in each sample, and forming 1 group of monitoring columns by root growth columns, litter degradation columns and contrast columns; another application scenario is: on the grassland planted with the same variety of carex, 6 treatments of 0mm (rain-fed), 20mm, 50mm, 100mm, 150mm and 200mm are set according to annual irrigation quantity, 3 samples of 0.5m multiplied by 0.5m are respectively randomly selected from each treated grassland, 18 samples are provided in total, 2 groups of monitoring columns are arranged in each sample, each group of monitoring columns consists of root system growing columns and comparison columns, the litter is regularly cleaned away, the quantity of organic carbon conveyed to soil by the litter is very small, so that the litter degrading columns do not need to be arranged, the monitoring columns are arranged in the selected 18 samples when the first annual growth season begins, and a group of monitoring columns in each sample are respectively taken back when the second and third annual growth seasons begin to perform indoor index measurement so as to monitor the influence of water-saving irrigation measures on the increase of organic carbon storage quantity of soil in gardens.
The method provided by the embodiment provides specification ranges of the monitoring columns in different experimental areas, provides deployment modes of the monitoring columns in different application scenes, and can comprehensively measure the net contribution amount of organic carbon transported to soil by plants in different scenes.
Optionally, the filler is a mixture of sand and soil, wherein the volume ratio of the sand to the soil is 1: 1.
Specifically, in order to facilitate the quantitative calculation of the variation amount of the organic carbon transported to the soil by implanting into each monitoring column, the filler in each monitoring column is set to be sand and soil with equal volumes, wherein the sand does not contain carbon, and the carbon stable isotope ratio of the soil is significantly different from the carbon stable isotope ratio of the plant transporting the organic carbon to the soil, for example, if the plant transporting the organic carbon is C3Plants, the soil being taken from C4Land where plants grow throughout the year (more than 20 years), C4Plants including corn, sugarcane, and the like; if the plant delivering organic carbon is C4Plants, the soil being taken from C3Land where plants grow throughout the year C3The plant includes wheat, rice, etc.
The embodiment provides the components of the filler in the monitoring columns, and provides convenience for quantitatively calculating the variable quantity of organic carbon transported to soil by plants in each monitoring column.
The following description will be made with reference to fig. 3 for an apparatus for measuring organic carbon transport to soil by plants in different routes according to an embodiment of the present invention, and the following description will be made with reference to the above-described method for measuring organic carbon transport to soil by plants in different routes.
The invention provides a device for measuring organic carbon delivery to soil by plants in different ways, which comprises:
the first determination module 301 is configured to determine an initial volume weight of a filler in a preset monitoring column, where the monitoring column includes a root growth column, a litter degradation column, and a control column; a second determination module 302, configured to bury the monitoring column in target soil, take out the monitoring column after at least two growth cycles to obtain a target monitoring column, and determine a target organic carbon content and a target carbon stable isotope ratio according to the target monitoring column; and the calculating module 303 is configured to input the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column, and the initial volume weight into a pre-stored isotope binary mixture model for calculation, so as to obtain a net contribution amount of the plant to the target soil for conveying organic carbon through different approaches.
The invention provides a device for measuring organic carbon delivery to soil by plants in different ways, which comprises a first measuring module 301 for measuring the initial volume weight of fillers in a preset monitoring column, wherein the monitoring columns include root growth columns, litter degradation columns, and control columns, and are then buried in target soil by the second determination module 302, taken out after at least two growth cycles, to obtain a target monitoring column, respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column, finally inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model through a calculation module 303 for calculation, to obtain the net contribution of the plant to the organic carbon transported to the target soil by different ways; the device of the embodiment can realize the quantitative monitoring of organic carbon transported to soil by plants, and improves the reliability of carbon sink amount accounting of a land ecosystem.
Fig. 4 illustrates a physical structure diagram of an electronic device, which may include, as shown in fig. 4: a processor (processor)410, a communication Interface 420, a memory (memory)430 and a communication bus 440, wherein the processor 410, the communication Interface 420 and the memory 430 are communicated with each other via the communication bus 440. Processor 410 may invoke logic instructions in memory 430 to perform a method for determining the delivery of organic carbon to soil by plants based on different pathways, the method comprising: measuring the initial volume weight of a filler in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column; burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; and inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to conveying organic carbon into the target soil through different ways.
In addition, the logic instructions in the memory 430 may be implemented in the form of software functional units and stored in a computer readable storage medium when the software functional units are sold or used as independent products. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.
The present invention also provides a non-transitory computer readable storage medium having stored thereon a computer program which when executed by a processor implements a method for determining organic carbon delivery to soil based on plants in different pathways provided by the above methods, the method comprising: measuring the initial volume weight of a filler in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column; burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; and inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to conveying organic carbon into the target soil through different ways.
The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.
Through the above description of the embodiments, those skilled in the art will clearly understand that each embodiment can be implemented by software plus a necessary general hardware platform, and certainly can also be implemented by hardware. With this understanding in mind, the above-described technical solutions may be embodied in the form of a software product, which can be stored in a computer-readable storage medium such as ROM/RAM, magnetic disk, optical disk, etc., and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute the methods described in the embodiments or some parts of the embodiments.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. The method for determining organic carbon delivery to soil by plants based on different ways is characterized by comprising the following steps:
measuring the initial volume weight of fillers in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column;
burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column;
and inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to conveying organic carbon into the target soil through different ways.
2. The method for determining organic carbon delivery to soil by plants in different ways according to claim 1, wherein the step of determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column comprises the following steps:
respectively measuring a first organic carbon content and a first carbon stable isotope ratio of fillers in a root system growth column of the target monitoring column, and respectively measuring a second organic carbon content and a second carbon stable isotope ratio of fillers in a litter degradation column of the target monitoring column and a third carbon stable isotope ratio of fillers in a contrast column of the target monitoring column;
and respectively measuring the fourth carbon stable isotope ratio of the thin root in the root system growth column of the target monitoring column and the fifth carbon stable isotope ratio of the litter on the litter degrading column of the target monitoring column.
3. The method for determining organic carbon delivery to soil by plants via different routes according to claim 1, wherein the monitoring column is buried in target soil, and is taken out after at least two growth cycles to obtain the target monitoring column, and the method comprises:
setting the side wall and the upper and lower bottom surfaces of the root system growth column as plastic gauze with the aperture of 2mm, respectively setting the side walls of the litter degradation column and the contrast column as PVC pipes, and setting the lower bottom surfaces of the litter degradation column and the contrast column as compact nets with the aperture of 1 mu m;
simultaneously placing the arranged root growth column, the litter degradation column and the contrast column in corresponding pot holes for burying, and respectively arranging isolation covers with the aperture of 5mm above the root growth column and the contrast column;
and removing the litter on the isolation cover according to a preset fixed period, and taking out the monitoring column after reaching a preset growth period so as to obtain the target monitoring column.
4. The method for determining organic carbon delivery to soil by plants according to different routes as claimed in claim 1, wherein the target organic carbon content, the target carbon stable isotope ratio, the target monitoring column height and the initial volume weight are input into a pre-stored isotope binary mixture model for calculation so as to obtain the net contribution of the plants to organic carbon delivery to the target soil by different routes, specifically comprising:
inputting the target carbon stable isotope ratio into the model, and obtaining a target proportion according to the following formula:
Frt＝(δ13Cingrowth-δ13Ccontrol)/(δ13Crt-δ13Ccontrol)
Flitter＝(δ13Cdegradation-δ13Ccontrol)/(δ13Clitter-δ13Ccontrol)
wherein, FrtAnd FlitterRespectively representing the proportion of soil carbon from root systems and withered and fallen objects; delta13Cingrowth、δ13CdegradationAnd delta13CcontrolRespectively representing the carbon isotope ratios of fillers in the root growth column, the litter degrading column and the contrast column; delta13CrtAnd delta13ClitterRespectively representing the carbon isotope ratios of the root system in the root system growing column and the litter on the litter degrading column;
inputting the target proportion, the target organic carbon content, the height of the target monitoring column and the initial volume weight into the model, and obtaining the net contribution of the plant to conveying organic carbon into the target soil through different ways according to the following formula:
Crd-net＝ρ×[C]ingrowth×Frt×h×10000
Cld-net＝ρ×[C]degradation×Flitter×h×10000
wherein, Crd-netAnd Cld-netRespectively representing the net contribution amount of root system input and litter degradation to soil carbon, wherein rho is the initial volume weight of the filler in the monitoring column, [ C]ingrowthAnd [ C]degratationRespectively representing the organic carbon content of the fillers in the root growth column and the litter degradation column during sampling, h is the height of the monitoring column, and 10000 is a unit from the organic carbon content to the net contribution amountAnd (5) converting the coefficient.
5. The method according to claim 1, wherein the target organic carbon content is determined by an elemental analyzer, the initial volume weight is determined by a ring cutter method, and the target carbon stable isotope ratio is determined by a carbon stable isotope analyzer.
6. The method for determining organic carbon delivery to soil by plants according to claim 1, wherein the height of the target monitoring column is within 30cm to 50 cm.
7. The method for determining organic carbon delivery to soil by plants according to claim 1, wherein the filler is a mixture of sand and soil, and the volume ratio of the sand to the soil is 1: 1.
8. An apparatus for determining organic carbon delivery to soil based on plants in different pathways, the apparatus comprising:
the device comprises a first determination module, a second determination module and a third determination module, wherein the first determination module is used for determining the initial volume weight of a filler in a preset monitoring column, and the monitoring column comprises a root system growth column, a litter degradation column and a control column;
the second determination module is used for burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain the target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column;
and the calculation module is used for inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to the organic carbon conveyed into the target soil through different ways.
9. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, wherein the processor executes the program to implement the method for determining the transport of organic carbon to soil based on plants in different pathways according to any one of claims 1 to 7.
10. A non-transitory computer readable storage medium having stored thereon a computer program, wherein the computer program when executed by a processor implements the method for determining the transport of organic carbon by plants to soil based on different pathways according to any one of claims 1 to 7.
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