CN114544911A - Method and device for determining organic carbon delivery from plants to soil based on different ways - Google Patents

Abstract

The invention provides a method and a device for determining organic carbon delivery to soil by plants in different ways, wherein the method comprises the following steps: measuring the initial volume weight of a filler in a preset monitoring column, wherein the monitoring column comprises a root growth column, a litter degradation column and a control column; burying the monitoring column in target soil, taking out the monitoring column after at least two growth cycles to obtain a target monitoring column, and respectively determining the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; and inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial volume weight into a pre-stored isotope binary mixed model for calculation so as to obtain the net contribution of the plant to conveying organic carbon into the target soil through different ways.

Description

Method and device for the determination of organic carbon transport from plants to soil based on different pathways

technical field

The invention belongs to the technical field of soil component detection, and in particular relates to a method and a device for measuring organic carbon transported from plants to soil based on different pathways.

Background technique

Soil organic carbon mainly comes from plants, and litter degradation and root secretion and turnover are two important ways for plants to transport organic carbon to soil.

Due to the complexity of plant-soil interactions, there is a lack of uniform quantification methods for organic carbon transport from plants to soil and the contribution of litter and root pathways to organic carbon transport in existing technologies, such as: existing technologies for forest ecosystems The main accounting content of carbon sinks is forest stock, and there is no accounting for soil organic carbon increase; although the aboveground biomass of grassland ecosystems is mainly converted into forage and carbon is released, no-tillage supplementary seeding for degraded grasslands The increase in soil organic carbon storage by man-made interventions such as seasonal rest grazing is not yet clear, so it is impossible to assess the carbon sink effect of grassland ecological quality and productivity improvement technologies, resulting in an unreliable accounting process for carbon sinks in terrestrial ecosystems.

SUMMARY OF THE INVENTION

The invention provides a method and device for measuring organic carbon transported by plants to soil based on different pathways, which is used to solve the problem that there is no uniform quantitative method for measuring the organic carbon transported by plants to soil in the prior art, which leads to the process of carbon sink accounting Incomplete and imperfect defects improve the reliability of the process of accounting for carbon sinks in terrestrial ecosystems.

The present invention provides a method for determining the transport of organic carbon from plants to soil based on different pathways, the method comprising:

Determining the initial bulk density of the filling in a preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column; the monitoring column is buried in the target soil, and after at least two growth cycles Then take out the monitoring column to obtain the target monitoring column, and respectively measure the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; the target organic carbon content, the target carbon stable isotope ratio, the target carbon stable isotope ratio, and The height of the target monitoring column and the initial bulk density are input into the pre-stored isotope binary mixing model for calculation, so as to obtain the net contribution of plants to transport organic carbon into the target soil through different pathways.

According to a kind of determination method based on different pathways of plants transporting organic carbon to soil provided by the present invention, the method also includes:

respectively measuring the first organic carbon content and the first carbon stable isotope ratio of the packing in the root growth column of the target monitoring column, and respectively measuring the second organic carbon of the packing in the litter degradation column of the target monitoring column content and the second carbon stable isotope ratio and the third carbon stable isotope ratio of the filler in the control column of the target monitoring column; respectively determine the fourth carbon stable isotope ratio of the fine roots in the root growth column of the target monitoring column and The fifth carbon stable isotope ratio of the litter on the litter degradation column of the target monitoring column.

The side walls and the upper and lower bottom surfaces of the root growth column are set as plastic gauze with a hole diameter of 2 mm, the side walls of the litter degradation column and the control column are respectively set as PVC pipes, and the The lower bottom surface of the litter degradation column and the control column is set as a dense mesh with a pore diameter of 1 μm; the set root growth column, the litter degradation column and the control column are placed in the corresponding pits for burial at the same time. An isolation hood with a pore diameter of 5 mm is set above the root growth column and the control column respectively; the litter on the isolation hood is removed according to a preset fixed period, and the monitoring device is taken out after reaching the preset growth period. column to obtain the target monitoring column.

Input the target carbon stable isotope ratio into the model, and obtain the target ratio according to the following formula:

F _rt =(δ ¹³ C _ingrowth -δ ¹³ C _control )/(δ ¹³ C _rt -δ ¹³ C _control )

_Flitter =(δ ¹³ C _degradation -δ ¹³ C _control )/(δ ¹³ C _litter -δ ¹³ C _control )

Among them, F _rt and Flitter represent the proportion of soil carbon originating from roots and _litter , respectively; δ ¹³ C _ingrowth , δ ¹³ C _degradation and δ ¹³ C _control represent the root growth column and the litter degradation column, respectively and the carbon isotope ratio of the filler in the control column; δ ¹³ C _rt and δ ¹³ C _litter represent the carbon isotope ratio of the root system in the root growth column and the litter on the litter degradation column, respectively; The target ratio, the target organic carbon content, the height of the target monitoring column and the initial bulk density are input into the model, and the net amount of organic carbon transported by plants to the target soil through different ways is obtained according to the following formula. Contribution:

C _rd-net =ρ×[C] _ingrowth ×F _rt ×h×10000

C _ld-net =ρ×[C] _degradation ×F _litter ×h×10000

Among them, C _rd-net and C _ld-net represent the net contribution of root input and litter degradation to soil carbon, respectively, ρ is the initial bulk density of the packing in the monitoring column, [C] _ingrowth and [C] _degratation Represents the organic carbon content of the root growth column and the litter degradation column when the packing is sampled, h is the height of the monitoring column, and 10000 is the unit conversion coefficient from the organic carbon content to the net contribution.

The content of the target organic carbon is determined by an elemental analyzer, the initial bulk density is determined by a ring knife method, and the ratio of the target carbon stable isotope is determined by a carbon stable isotope analyzer.

The height of the target monitoring column is within 30cm-50cm.

The filling is a mixture of sand and soil, wherein the volume ratio of the sand and the soil is 1:1.

The present invention also provides a measuring device for transferring organic carbon from plants to soil based on different pathways, the device comprising:

The first measurement module is used to measure the initial bulk density of the filling in the preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column; the second measurement module is used to measure the The monitoring column is buried in the target soil, and after at least two growth cycles, the monitoring column is taken out to obtain the target monitoring column, and the target organic carbon content and the target carbon stable isotope ratio are respectively determined according to the target monitoring column; the calculation module, It is used to input the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial bulk density into the pre-stored isotope binary mixture model for calculation, so as to obtain the plant to the The net contribution of transported organic carbon in the target soil.

The present invention also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and running on the processor, when the processor executes the program, the processor implements any of the above based on different approaches Methods for the determination of organic carbon transport from plants to soil.

The present invention also provides a non-transitory computer-readable storage medium on which a computer program is stored, and when the computer program is executed by a processor, realizes the method for determining the transport of organic carbon to soil based on different pathways of plants according to any of the above-mentioned methods .

The present invention provides a method and device for the determination of organic carbon transport from plants to soil based on different pathways. First, the initial bulk density of the filler in a preset monitoring column is measured, wherein the monitoring column includes a root growth column, a litter degradation column, and a root growth column. column and control column, then the monitoring column is buried in the target soil, after at least two growth cycles, the monitoring column is taken out to obtain the target monitoring column, and the target organic carbon content and The target carbon stable isotope ratio, and finally the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial bulk density are input into the pre-stored isotope binary mixture model for calculation to obtain The net contribution of organic carbon transported by plants to the target soil through different paths; the invention provides a method for quantitative monitoring of the transport of organic carbon from plants to soil, which improves the reliability of accounting for carbon sinks in terrestrial ecosystems .

Description of drawings

In order to explain the present invention or the technical solutions in the prior art more clearly, the following will briefly introduce the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are the For some embodiments of the invention, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without any creative effort.

Fig. 1 is a schematic flow sheet of a method for measuring organic carbon transported to soil by plants based on different pathways provided by the embodiment of the present invention;

2 is a schematic structural diagram of a group of monitoring columns provided by another embodiment of the present invention;

3 is a schematic structural diagram of a measuring device for delivering organic carbon to soil based on different pathways of plants provided in an embodiment of the present invention;

FIG. 4 is a schematic structural diagram of an electronic device provided by the present invention.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention. , not all examples. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Below in conjunction with Fig. 1, the method for determining the transport of organic carbon from plants to soil based on different pathways provided in the embodiment of the present invention is described, including:

Step 101: Measure the initial bulk density of the packing in a preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column.

Since soil organic carbon mainly comes from the two important pathways of plant litter degradation and root secretion and turnover, in this example, a set of plant-to-soil-to-soil columns composed of root growth columns, litter degradation columns and control columns were set up. A monitoring column for quantifying the delivered organic carbon, among which, the root growth column is used to monitor the change of organic carbon transported by plants to the soil through root secretion and turnover. Contribution of organic carbon transport to soil through root exudation and turnover; litter degradation column is used to monitor the change of organic carbon transported to soil by plants through litter degradation, which can be compared with the organic carbon filling in the control column. Combined with the calculation of carbon changes, the contribution of plants to transport organic carbon to soil through litter degradation; the filling in the control column without root secretion and turnover and litter degradation transported organic carbon to it, as this. Example: The control group in the monitoring column; in this example, before burying the above-mentioned monitoring column in the corresponding pit, the initial bulk density of the filler in the preset monitoring column was measured, specifically: degrading the root growth column and litter. The packing of the column and the control column is filled with the ring knife (volume is 100cm ³ ), and the weight of the ring knife before and after the packing is weighed, and the corresponding weight is accurate to 0.01g, so as to obtain the initial bulk density of the packing in the monitoring column.

Step 102, bury the monitoring column in the target soil, take out the monitoring column after at least two growth cycles to obtain the target monitoring column, and measure the target organic carbon content and target carbon stability according to the target monitoring column respectively. isotope ratio.

Specifically, in this example, before the growth cycle of the plant starts, the initial bulk density of the filler in the monitoring column is obtained, and then the root growth column, the litter degradation column and the control column are buried in different potholes in the soil of the experimental area respectively. After at least two growth cycles, the buried monitoring column is taken out to measure the change of organic carbon contained in the packing in the taken-out monitoring column, specifically: measuring the packing in each monitoring column after taking out The organic carbon content and carbon stable isotope ratio of organic carbon, the carbon stable isotope ratio of plant fine roots in the root growth column, and the carbon stable isotope ratio of litter on the litter degradation column, etc. The net contribution of soil transported organic carbon is facilitated.

It should be noted that the growth cycle of different plants may be different, and the plant growth cycle adopted in this embodiment is one year.

Step 103: Input the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column, and the initial bulk density into the pre-stored isotope binary mixture model for calculation, so as to obtain plants through different ways. The net contribution of delivering organic carbon to the target soil.

It can be understood that in this example, the organic carbon content and carbon stable isotope ratio of the filler in the root growth column after being taken out, the organic carbon content and carbon stable isotope ratio of the filler in the litter degradation column, and the carbon stable isotope ratio in the control column are obtained. The carbon stable isotope ratio of the filler, the carbon stable isotope ratio of the fine roots in the root growth column, and the carbon stable isotope ratio of the litter on the litter degradation column, and then these parameters are combined with the pre-determined height of the monitoring column. h and the initial bulk density ρ of the filling of the monitoring column are input into the isotope binary mixing model to calculate the net contribution of the plants in the experimental area to transport organic carbon to the soil; it should be noted that the monitoring column includes root growth column, litter degradation The measured height h of the column and the control column should be consistent, and the initial bulk density ρ of the packing of the root growth column, the litter degradation column and the control column should also be consistent.

This embodiment provides a method for quantitatively monitoring the transport of organic carbon from plants to soil, which improves the reliability of accounting for carbon sinks in terrestrial ecosystems.

Optionally, the first organic carbon content and the first carbon stable isotope ratio of the filler in the root growth column of the target monitoring column are respectively measured, and the content of the filler in the litter degradation column of the target monitoring column is respectively measured. The second organic carbon content and the second carbon stable isotope ratio and the third carbon stable isotope ratio of the filler in the control column of the target monitoring column; the fourth carbon content of the fine roots in the root growth column of the target monitoring column is respectively determined The stable isotope ratio and the fifth carbon stable isotope ratio of the litter on the litter degradation column of the target monitoring column.

It can be understood that in this example, the organic carbon content and carbon stable isotope ratio of the filler in the root growth column after removal, the organic carbon content and carbon stable isotope ratio of the filler in the litter degradation column, and the filler in the control column were determined. The carbon stable isotope ratio of the litter, the carbon stable isotope ratio of the fine roots in the root growth column, and the carbon stable isotope ratio of the litter on the litter degradation column.

Specifically, in this example, after each growth cycle of plants in the experimental area is over, a litter sample within a range of 0.5m × 0.5m near the installation point of the monitoring column is collected; then after at least two growth cycles, from the installation point The root growth column, the litter degradation column and the control column were dug out at the same time, and the filling samples in the columns were collected respectively, and the root samples in the root growth column were collected; Soil animals, and then wash the topsoil in deionized water to obtain fresh litter samples, and then bake at 70 °C for 72 hours to obtain dry litter samples and weigh them. The weighing mass of the litter samples is accurate to 0.01g. Obtain the amount of litter; for plant roots, in this example, the filler was first separated from the plant roots using a 2mm sieve, then dead roots were removed, and live roots were washed with deionized water to obtain fresh root samples, and then dried at 70 ° C for 72h, The dry root sample is obtained and weighed, the weight of the root sample is accurate to 0.01g, and finally the plant root biomass is obtained; for the filler, in this example, the root system was first removed from the root growth column filler and degraded from the litter. The litter was removed from the top of the column to obtain the root growth column, litter degradation column and packing samples in the control column, and then air-dried to obtain dry packing samples; After filling the samples, this example uses auxiliary tools to measure these samples to obtain the required organic carbon content and carbon stable isotope ratio.

This embodiment provides a method for extracting the target organic carbon content and the target carbon stable isotope ratio from the removed monitoring column, which provides convenience for the subsequent process to calculate the net contribution of plants to the soil through different ways of transporting organic carbon.

Optionally, the side walls and the upper and lower bottom surfaces of the root growth column are set as plastic gauze with a hole diameter of 2 mm, and the side walls of the litter degradation column and the control column are respectively set as PVC pipes, The lower bottom surface of the litter degradation column and the control column is set as a dense mesh with a pore diameter of 1 μm; the set root growth column, litter degradation column and control column are placed in the corresponding pits at the same time. Bury, and set isolation hoods with a diameter of 5 mm above the root growth column and the control column respectively; remove the litter on the isolation hood according to a preset fixed period, and after reaching the preset growth period Remove the monitoring column to obtain the target monitoring column.

Specifically, the structure of each group of monitoring columns is shown in FIG. 2 . In this embodiment, the root growth column 201 , the litter degradation column 202 and the control column 203 are embedded side by side in three pre-set potholes in the experimental area. , wherein, the above-mentioned three columns are in seamless contact with the side walls and the bottom of the corresponding potholes, and the spacing between adjacent potholes is within the range of 10cm-20cm, and the distance between adjacent potholes is twice the diameter of the bottom surface of the monitoring column. , that is, the diameter of the bottom surface of the above-mentioned three columns is in the range of 5cm-10cm; then the side wall and the upper and lower bottom surfaces of the root growth column 201 are set with a plastic gauze with a hole diameter of 2mm, so that the internal filler does not escape, and ensure the fine roots of the plant. Able to grow inside the column, place the root growth column in the pit, cover the top of it with an isolation cover with a hole diameter of 5mm, and remove the litter on the isolation cover at a weekly or monthly fixed cycle to prevent The litter is in contact with the filler in the column, the side wall of the litter degradation column 202 is set as a PVC pipe, and the bottom surface of the litter is set as a dense mesh with a pore size of 1 μm, so that plant roots and soil fungi cannot enter the interior of the column, while ensuring that The water and heat exchange in the vertical direction is not blocked. After the litter degradation column 202 is buried in the pothole, there is no shielding on the top of the litter, and the litter contacts the filling in the column and accumulates and degrades naturally. The side wall of the control column 203 is set as a PVC pipe, and the bottom surface of the control column 203 is a dense mesh with a hole diameter of 1 μm. Or remove the litter on the isolation hood in a fixed period every month; finally, after two growth cycles (two years), take out the target monitoring column from the experimental area, that is, the root growth column 201 and the litter degradation column taken out 202 and the control column 203, at this time, the root growth column 201 has organic carbon that is transported by the plant roots in the experimental area to the filling in the column, and the litter degradation column 202 is degraded by the litter of the plants in the experimental area. The organic carbon delivered by the packing in the column, the control column 203 was used as the control group for this experiment.

This embodiment provides a method for burying the monitoring columns after differential setting to obtain a target monitoring column. The method adopts the control variable method and sets a control group, and obtains dry litter samples and dry roots from the target monitoring column for the subsequent process. Samples and dry fill samples thus provide convenience for calculating the net contribution of plants to soil organic carbon transport through different pathways.

Optionally, the target carbon stable isotope ratio is input into the model, and the target ratio is obtained according to the following formula:

F _rt =(δ ¹³ C _ingrowth -δ ¹³ C _control )/(δ ¹³ C _rt -δ ¹³ C _control )

_Flitter =(δ ¹³ C _degradation -δ ¹³ C _control )/(δ ¹³ C _litter -δ ¹³ C _control )

Among them, F _rt and Flitter represent the proportion of soil carbon originating from roots and _litter , respectively; δ ¹³ C _ingrowth , δ ¹³ C _degradation and δ ¹³ C _control represent the root growth column and the litter degradation column, respectively and the carbon isotope ratio of the filler in the control column; δ ¹³ C _rt and δ ¹³ C _litter represent the carbon isotope ratio of the root system in the root growth column and the litter on the litter degradation column, respectively; The target ratio, the target organic carbon content, the height of the target monitoring column and the initial bulk density are input into the model, and the net contribution of organic carbon in the target soil is obtained according to the following formula:

C _rd-net =ρ×[C] _ingrowth ×F _rt ×h×10000

C _ld-net =ρ×[C] _degradation ×F _litter ×h×10000

Among them, C _rd-net and C _ld-net represent the net contribution of root input and litter degradation to soil carbon, respectively, ρ is the initial bulk density of the packing in the monitoring column, [C] _ingrowth and [C] _degratation Represents the organic carbon content of the root growth column and the litter degradation column when the packing is sampled, h is the height of the monitoring column, and 10000 is the unit conversion coefficient from the organic carbon content to the net contribution.

Specifically, in this example, the carbon stable isotope ratio δ ¹³ C _ingrowth of the filler in the root growth column, the carbon stable isotope ratio δ ¹³ C _control of the filler in the control column, and the carbon stable isotope ratio of the plant root in the root growth column δ ¹³ C _rt into the following formula:

F _rt =(δ ¹³ C _ingrowth -δ ¹³ C _control )/(δ ¹³ C _rt -δ ¹³ C _control )

Calculate the proportion of soil carbon originating from the root system F _rt , that is, the proportion of organic carbon transported by plants to the soil through the root system, and calculate the F _rt , the initial bulk density ρ of the filling, and the organic carbon content in the root growth column when sampling the filling [C] The _ingrowth and the height h of the monitoring column are substituted into the following formula:

C _rd-net =ρ×[C] _ingrowth ×F _rt ×h×10000

The net contribution C _rd-net of root input to soil carbon in this example is obtained, that is, the net contribution of plant roots to soil organic carbon; at the same time, in this example, the carbon isotope ratio δ of the filler in the litter degradation column is obtained. ¹³ C _degradation , the carbon stable isotope ratio of the packing in the control column δ ¹³ C _control and the carbon isotope ratio of the litter on the litter degradation column δ ¹³ C _litter are substituted into the following formula:

_Flitter =(δ ¹³ C _degradation -δ ¹³ C _control )/(δ ¹³ C _litter -δ ¹³ C _control )

Calculate the proportion of soil carbon originating from _litter , Flitter, that is, the proportion of organic carbon transported to soil by plants through _litter degradation. The organic carbon content [C] _degratation and the height h of the monitoring column are substituted into the following formula:

C _ld-net =ρ×[C] _degradation ×F _litter ×h×10000

The net contribution C _ld-net of litter degradation to soil carbon in this example is obtained, that is, the net contribution of plant litter to soil organic carbon input. In this example, the root input of plants and litter The unit of the net contribution of degradation to soil carbon is g C·m ^-2 , the unit of initial bulk density is g·m ^-3 , the unit of organic carbon content is percentage (%), and the unit of monitoring column height is cm; It is noted that, in this embodiment, the root growth column, the litter degradation column and the control column included in the target monitoring column have the same measurement heights.

This embodiment provides a method for obtaining the net contribution of plants to soil organic carbon based on carbon stable isotope ratio and organic carbon content, which can effectively quantify the contribution of litter and roots to plants increasing soil organic carbon storage.

Optionally, use an elemental analyzer to measure the target organic carbon content, use a ring knife method to measure the initial bulk density, and use a carbon stable isotope analyzer to measure the target carbon stable isotope ratio.

It can be understood that, in order to extract the required organic carbon content and carbon stable isotope ratio from the obtained dry litter samples, dry root samples and dry filler samples, in this embodiment, a ball mill is used to pulverize the dry litter samples, dry Root samples and dry filler samples were then measured by an elemental analyzer to obtain carbon content and nitrogen content, and measured by a stable isotope analyzer to obtain the carbon stable isotope ratio (δ ¹³ C); in addition, in this embodiment, the ring knife method was used to measure and obtain The initial bulk density of the column packing was monitored prior to burial.

The method described in this example provides a method for obtaining the organic carbon content, carbon stable isotope ratio and initial bulk density, which provides convenience for the subsequent process to calculate the net contribution of organic carbon transported by plants to the soil through different channels.

Optionally, the height of the target monitoring column is within 30cm-50cm.

It can be understood that the soil quality, plant species and vegetation coverage are different in different experimental areas. Therefore, in this embodiment, the root growth column, the litter degradation column and the control column included in the monitoring column are set to the same bottom surface size and size. Height, its height is in the range of 30cm-50cm, the diameter of the bottom surface is in the range of 5cm-10cm, and the specific height can be flexibly selected according to different experimental areas; Determination of organic carbon transport to soil, for example, an application scenario is: randomly select three 1m × 1m quadrats in the no-tillage replanting grassland and the control grassland (grassland without no-tillage replanting measures), a total of 6 Two groups of monitoring columns were arranged in each sample square. The root growth column, the litter degradation column and the control column constituted one group of monitoring columns. At the beginning of the first growing season, the monitoring columns were arranged in the selected In 6 quadrats, and at the beginning of the second and third year growing seasons, a set of monitoring columns in each quadrat were retrieved for indoor index measurement to monitor the carbon sequestration effect of no-tillage supplementary sowing measures; Another application scenario is: in the grassland planted with the same variety of sedge grass, set 0mm (rain-fed), 20mm, 50mm, 100mm, 150mm, 200mm according to the annual irrigation amount, a total of 6 treatments, each treatment grassland is randomly selected 3 0.5 There are 18 squares in m×0.5m square, and 2 groups of monitoring columns are arranged in each square. Each group of monitoring columns is composed of a root growth column and a control column. The amount of organic carbon transported to the soil is very small, so it is not necessary to install litter degradation columns. At the beginning of the three-year growing season, a set of monitoring columns in each quadrat were retrieved and measured indoors to monitor the effect of water-saving irrigation measures on the increase of soil organic carbon storage in garden green space.

The method described in this embodiment provides the specification range of the monitoring columns in different experimental areas, and provides the deployment methods of the monitoring columns in different application scenarios, which can comprehensively measure the net contribution of plants to soil organic carbon in different scenarios.

Optionally, the filler is a mixture of sand and soil, wherein the volume ratio of the sand and the soil is 1:1.

Specifically, in order to facilitate the quantitative calculation of the change in the amount of organic carbon that is implanted in each monitoring column and transported to the soil, in this embodiment, the filling in each monitoring column is set to be equal volumes of sand and soil, wherein the sand does not contain Carbon, and the carbon stable isotope ratio of the soil is significantly different from the carbon stable isotope ratio _of the plant that transports organic carbon to it _. For example, if the plant that transports organic carbon is a C Years) growing land, _C4 plants include corn, sugarcane, etc.; if the plants transporting organic carbon are _C4 plants, the soil is taken from the land where C3 plants grow all _{year round} , and C3 plants include wheat, rice, etc.

This embodiment provides the components of the packing in the monitoring column, which facilitates the quantitative calculation of the change in the amount of organic carbon transported by plants to the soil in each monitoring column.

With reference to FIG. 3 , an assay device for delivering organic carbon from plants to soil based on different pathways provided by an embodiment of the present invention will be described. The assay device described below for delivering organic carbon from plants to soil through different pathways is the same as the one described above. The determination methods based on different pathways of plant-to-soil transport of organic carbon can be referred to each other.

The present invention provides a device for measuring organic carbon transport from plants to soil based on different pathways, the device comprising:

The first measurement module 301 is used to measure the initial bulk density of the filler in the preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column; the second measurement module 302 is used to The monitoring column is buried in the target soil, and after at least two growth cycles, the monitoring column is taken out to obtain the target monitoring column, and the target organic carbon content and the target carbon stable isotope ratio are respectively determined according to the target monitoring column; calculating Module 303, for inputting the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial bulk density into a pre-stored isotope binary mixture model for calculation, so as to obtain the The net contribution of different pathways to the delivery of organic carbon to the target soil.

The present invention provides a measuring device for transferring organic carbon from plants to soil in different ways. First, the first measuring module 301 is used to measure the initial bulk density of the filler in a preset monitoring column, wherein the monitoring column includes a root growth column, The litter degradation column and the control column are then buried in the target soil through the second determination module 302, and the monitoring column is taken out after at least two growth cycles to obtain the target monitoring column, and according to the The target monitoring column measures the target organic carbon content and the target carbon stable isotope ratio respectively, and finally inputs the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial bulk density through the calculation module 303 The pre-stored isotope binary mixture model is used for calculation to obtain the net contribution of plants to the target soil through different ways. , improving the reliability of accounting for carbon sinks in terrestrial ecosystems.

FIG. 4 illustrates a schematic diagram of the physical structure of an electronic device. As shown in FIG. 4 , the electronic device may include: a processor (processor) 410, a communication interface (Communications Interface) 420, a memory (memory) 430, and a communication bus 440, The processor 410 , the communication interface 420 , and the memory 430 communicate with each other through the communication bus 440 . The processor 410 can invoke the logic instructions in the memory 430 to execute a method for determining the organic carbon delivered to the soil by plants based on different pathways, the method comprising: determining a preset initial bulk density of the filling in the monitoring column, wherein the The monitoring column includes a root growth column, a litter degradation column and a control column; the monitoring column is buried in the target soil, and after at least two growth cycles, the monitoring column is taken out to obtain the target monitoring column, and according to the The target monitoring column measures the target organic carbon content and the target carbon stable isotope ratio respectively; the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial bulk density are input into the pre-stored isotope two. Calculations were performed in a meta-mixture model to obtain the net contribution of plants to transport organic carbon to the target soil through different pathways.

In addition, the above-mentioned logic instructions in the memory 430 can be implemented in the form of software functional units and can be stored in a computer-readable storage medium when sold or used as an independent product. Based on this understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art or the part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including several The instructions are used to cause a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, Read-Only Memory (ROM, Read-Only Memory), Random Access Memory (RAM, Random Access Memory), magnetic disk or optical disk and other media that can store program codes .

The present invention also provides a non-transitory computer-readable storage medium on which a computer program is stored, and when the computer program is executed by a processor, is implemented to execute a method for transporting organic carbon from plants to soil based on different pathways provided by the above methods. A determination method, the method comprising: determining the initial bulk density of the filler in a preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column; and burying the monitoring column in a target soil , after at least two growth cycles, take out the monitoring column to obtain the target monitoring column, and respectively measure the target organic carbon content and the target carbon stable isotope ratio according to the target monitoring column; The target carbon stable isotope ratio, the height of the target monitoring column, and the initial bulk density are input into the pre-stored isotope binary mixture model for calculation, so as to obtain the net contribution of plants to the target soil through different pathways. .

The device embodiments described above are only illustrative, wherein the units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in One place, or it can be distributed over multiple network elements. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution in this embodiment. Those of ordinary skill in the art can understand and implement it without creative effort.

From the description of the above embodiments, those skilled in the art can clearly understand that each embodiment can be implemented by means of software plus a necessary general hardware platform, and certainly can also be implemented by hardware. Based on this understanding, the above-mentioned technical solutions can be embodied in the form of software products, which can be stored in computer-readable storage media, such as ROM/RAM, magnetic disks, etc. , optical disc, etc., including several instructions to cause a computer device (which may be a personal computer, a server, or a network device, etc.) to perform the methods described in various embodiments or some parts of the embodiments.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that it can still be The technical solutions described in the foregoing embodiments are modified, or some technical features thereof are equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. the assay method of conveying organic carbon to soil based on different approaches plants, is characterized in that, comprises: Determining the initial bulk density of the packing in a preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column; Burying the monitoring column in the target soil, taking out the monitoring column after at least two growth cycles to obtain the target monitoring column, and measuring the target organic carbon content and the target carbon stable isotope ratio respectively according to the target monitoring column; The target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column, and the initial bulk density are input into the pre-stored isotope binary mixing model for calculation, so as to obtain the information that plants send to the target through different ways. Net contribution of transported organic carbon in the target soil. 2. the assay method of conveying organic carbon to soil based on different approach plants according to claim 1, is characterized in that, according to described target monitoring column, measure target organic carbon content and target carbon stable isotope ratio respectively, specifically comprise: respectively measuring the first organic carbon content and the first carbon stable isotope ratio of the packing in the root growth column of the target monitoring column, and respectively measuring the second organic carbon of the packing in the litter degradation column of the target monitoring column content and the second carbon stable isotope ratio and the third carbon stable isotope ratio of the packing in the control column of the target monitoring column; The fourth carbon stable isotope ratio of fine roots in the root growth column of the target monitoring column and the fifth carbon stable isotope ratio of the litter on the litter degradation column of the target monitoring column are respectively determined. 3. the assay method of transferring organic carbon to soil based on different approach plants according to claim 1, is characterized in that, described monitoring column is buried in target soil, after at least two growth cycles, take out described monitoring column, to obtain target monitoring columns, including: The side walls and the upper and lower bottom surfaces of the root growth column are set as plastic gauze with a hole diameter of 2 mm, the side walls of the litter degradation column and the control column are respectively set as PVC pipes, and the The lower bottom surfaces of the litter degradation column and the control column are set as dense meshes with a pore size of 1 μm; The set root growth column, litter degradation column and control column are placed in corresponding pits simultaneously for burial, and an isolation cover with a hole diameter of 5 mm is set above the root growth column and the control column respectively; The litter on the isolation cover is removed according to a preset fixed cycle, and the monitoring column is taken out after reaching the preset growth cycle to obtain the target monitoring column. 4. the assay method of transferring organic carbon to soil based on different pathways plants according to claim 1, is characterized in that, the height of described target organic carbon content, described target carbon stable isotope ratio, described target monitoring column and The initial bulk density is input into the pre-stored isotope binary mixing model for calculation to obtain the net contribution of plants to the target soil in transporting organic carbon through different pathways, specifically including: Input the target carbon stable isotope ratio into the model, and obtain the target ratio according to the following formula: F _rt =(δ ¹³ C _ingrowth -δ ¹³ C _control )/(δ ¹³ C _rt -δ ¹³ C _control ) _Flitter =(δ ¹³ C _degradation -δ ¹³ C _control )/(δ ¹³ C _litter -δ ¹³ C _control ) Among them, F _rt and Flitter represent the proportion of soil carbon originating from roots and _litter , respectively; δ ¹³ C _ingrowth , δ ¹³ C _degradation and δ ¹³ C _control represent the root growth column and the litter degradation column, respectively and the carbon isotope ratio of the filler in the control column; δ ¹³ C _rt and δ ¹³ C _litter represent the carbon isotope ratio of the root system in the root growth column and the litter on the litter degradation column, respectively; The target ratio, the target organic carbon content, the height of the target monitoring column and the initial bulk density are input into the model, and the following formula is used to obtain plants that transport organic carbon to the target soil through different ways Net contribution of: C _rd-net =ρ×[C] _ingrowth ×F _rt ×h×10000 C _ld-net =ρ×[C] _degradation ×F _litter ×h×10000 Among them, C _rd-net and C _ld-net represent the net contribution of root input and litter degradation to soil carbon, respectively, ρ is the initial bulk density of the packing in the monitoring column, [C] _ingrowth and [C] _degratation Represents the organic carbon content of the root growth column and the litter degradation column when the packing is sampled, h is the height of the monitoring column, and 10000 is the unit conversion coefficient from the organic carbon content to the net contribution. 5. the assay method of conveying organic carbon to soil based on different approach plants according to claim 1, is characterized in that, utilize elemental analyzer to measure described target organic carbon content, utilize ring knife method to measure described initial bulk density, utilize carbon A stable isotope analyzer measures the target carbon stable isotope ratio. 6 . The method for measuring organic carbon delivered by plants to soil based on different pathways according to claim 1 , wherein the height of the target monitoring column is within 30cm-50cm. 7 . 7. The assay method for transferring organic carbon from plants to soil based on different pathways according to claim 1, wherein the filler is a mixture of sand and soil, wherein the volume ratio of the sand and the soil is 1:1. 8. An assay device for transporting organic carbon from plants to soil based on different pathways, wherein the device comprises: a first determination module, used for determining the initial bulk density of the filling in a preset monitoring column, wherein the monitoring column includes a root growth column, a litter degradation column and a control column; The second measurement module is used for burying the monitoring column in the target soil, taking out the monitoring column after at least two growth cycles to obtain the target monitoring column, and measuring the target organic carbon content according to the target monitoring column. and the target carbon stable isotope ratio; The calculation module is used to input the target organic carbon content, the target carbon stable isotope ratio, the height of the target monitoring column and the initial bulk density into the pre-stored isotope binary mixture model for calculation, so as to obtain the plant pass The net contribution of different pathways to the delivery of organic carbon to the target soil. 9. An electronic device, comprising a memory, a processor and a computer program stored on the memory and running on the processor, wherein the processor implements the program as claimed in claim 1 when executing the program A method for determining the transport of organic carbon from plants to soil based on different pathways according to any one of to 7. 10. A non-transitory computer-readable storage medium on which a computer program is stored, characterized in that, when the computer program is executed by a processor, the different-path-based plant orientation according to any one of claims 1 to 7 is implemented. Methods for the determination of soil transported organic carbon.

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