CN102511362B - Method for utilizing double markers to acquire share of inorganic carbon source utilized by plants - Google Patents

Method for utilizing double markers to acquire share of inorganic carbon source utilized by plants Download PDF

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CN102511362B
CN102511362B CN2011103315211A CN201110331521A CN102511362B CN 102511362 B CN102511362 B CN 102511362B CN 2011103315211 A CN2011103315211 A CN 2011103315211A CN 201110331521 A CN201110331521 A CN 201110331521A CN 102511362 B CN102511362 B CN 102511362B
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吴沿友
邢德科
李海涛
刘莹
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Institute of Geochemistry of CAS
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Abstract

The invention discloses a method by utilizing double markers to acquire share of inorganic carbon source utilized by plants, which comprises the following steps that: A. sodium bicarbonate produced by different manufacturers is determined, two types of sodium bicarbonate with delta 13C difference being more than eight thousandths are used as an isotopic marker 1 and an isotopic marker 2 to be respectively added into nutrient fluid to culture plants; delta 13C value of bicarbonate ion in nutrient fluid of the isotopic marker 1 is delta C1, and delta 13C value of bicarbonate ion in nutrient fluid of the isotopic marker 2 is delta C2; and B. after more than four leaves appear, the values of delta 13C consisting of stable carbon isotopes of plant leaves to be observed correspondently cultured by the nutrient fluid of the two types of isotopic markers are respectively determined as delta T1 and delta T2; and C. delta C1, delta C2, delta T1 and delta T2 are brought into equation fB= to calculate the share fB of the carbonate ions utilized by the plants. Due to the adoption of the method, the share of the inorganic carbon source utilized by the plants can be rapidly acquired, the steps are fewer, simplicity in calculation can be realized, and data is reliable.

Description

Utilize double labelling to obtain the method for the inorganic carbon source share of plant utilization
Technical field
The present invention relates to a kind of method of utilizing stable carbon isotope to obtain the inorganic carbon source share of plant utilization, belong to the ecological environment treatment field.
Background technology
Plant can not only utilize the carbonic acid gas of air to carry out photosynthesis for raw material, and the bicarbonate ion that can utilize blade to store carries out photosynthesis for raw material.How many plants has significant correlation with the suitable natural disposition of the karst of plant to different inorganic utilization of carbon source shares, to screening karst adaptive plant, utilizes biological method to administer and recover fragile Karst ecosystem to have important effect.
The strong fractionation feature of stable carbon isotope is the basis in identification plant corpus DIC source.The occurring in nature carbon has two kinds of stable isotopes: 12C and 13C, their natural average abundance is respectively 98.89% and 1.11%.Stable carbon isotope forms uses δ usually 13C(‰) expression, occurring in nature δ 13C is changed to-90 ‰ ~+20 ‰.The strong fractionation feature of stable carbon isotope is conducive to identify the plant corpus DIC and originates.Mass balance principle and isotope mixed model and chemometrics method are the bases of quantitatively identifying DIC source in plant corpus.
The isotope mixed model of two end members can be expressed as:
δ T=δ A- f Bδ A +f Bδ B （1）
Here δ Tδ for plant leaf blade 13The C value, δ AThe δ of blade when utilizing carbonic acid gas for carbon source fully for being assumed to plant 13The C value, δ BThe δ of blade when utilizing bicarbonate ion for carbon source fully for being assumed to plant 13The C value, f BFor this investigation plant utilization bicarbonate ion is the shared share of carbon source.
Obviously, only know δ TBe difficult to obtain f B, therefore, the present invention adopts and utilizes the δ with larger difference 13C value bicarbonate ion is cultivated respectively plant simultaneously, identifies the share of the inorganic carbon source of plant utilization with the stable carbon isotope double labelling.
Principle of the present invention is as follows:
The isotope mixed model of two end members:
δ T=δ A- f Bδ A +f Bδ B (1)
For tagging 1, equation (1) is expressed as follows formula:
δ T1=δ A1- f B1δ A1 +f B1δ B1 （2）
Here δ T1For using the known δ of the first 13The δ of the plant leaf blade that the bicarbonate ion of C value is cultivated 13The C value, δ A1The δ of blade when utilizing carbonic acid gas for carbon source fully for being assumed to plant 13The C value, δ B1The δ of blade when utilizing this bicarbonate ion for carbon source fully for being assumed to plant 13The C value, f B1For this investigation plant utilization bicarbonate ion is the shared share of carbon source.
For tagging 2, equation (1) is expressed as follows formula:
δ T2=δ A2 - f B2δ A2 +f B2δ B2 （3）
Here δ T2For using the known δ of the second 13The δ of the plant leaf blade that the bicarbonate ion of C value is cultivated 13The C value, δ A2The δ of blade when utilizing carbonic acid gas for carbon source fully for being assumed to plant 13The C value, δ B2The δ of blade when utilizing this bicarbonate ion for carbon source fully for being assumed to plant 13The C value, f B2For this investigation plant utilization bicarbonate ion is the shared share of carbon source.
(2) δ and in (3) two equations A1=δ A2, f B=f B1=f B2, simultaneous solution
f B=
Figure 260739DEST_PATH_IMAGE001
（4）
(4) δ in formula B1-δ B2Can be converted into the δ of the bicarbonate ion of tagging 1 13C value δ C1δ with the bicarbonate ion of tagging 2 13C value δ C2Poor:
f B=
Figure 2011103315211100002DEST_PATH_IMAGE002
（5）
Therefore, can pass through the δ of the bicarbonate ion of mensuration tagging 1 13C value δ C1δ with the bicarbonate ion of tagging 2 13C value δ C2, measure simultaneously the δ of the plant leaf blade of cultivating with the bicarbonate ion of corresponding mark 13The C value namely determines δ T1And δ T2Value, (5) formula of complying with calculates the share f of plant utilization bicarbonate ion B
In prior art, from different experiments, the testing index that therefore causes is many, determination period is grown, step is comparatively loaded down with trivial details and evaluated error is larger due to the value of two end members.
Summary of the invention
The technical problem to be solved in the present invention is, a kind of method of utilizing double labelling to obtain the inorganic carbon source share of plant utilization is provided, to overcome in prior art value because of two end members from different experiments, the deficiency such as the testing index that causes is many, determination period long, step is comparatively loaded down with trivial details and evaluated error is larger.
The present invention takes following technical scheme: it comprises the following steps: the first, measure the sodium bicarbonate that different manufacturers is produced, and select two kinds of δ 13C value difference value is added to respectively as tagging 1 and tagging 2 greater than 8 ‰ sodium bicarbonate and is cultivated plant in nutrient solution; The δ of bicarbonate ion in tagging 1 culture fluid 13The C value is δ C1, the δ of bicarbonate ion in tagging 2 culture fluids 13The C value is δ C2The second, after cultivation has 4 above true leaves to length, measure respectively the corresponding stable carbon isotope composition δ that is investigated plant leaf blade that two kinds of isotope-labeled culture fluids are cultivated 13The value of C, δ T1And δ T2Value; The 3rd, with δ C1, δ C2, δ T1And δ T2Bring Equation f into B=
Figure 240196DEST_PATH_IMAGE002
, calculate the share f of plant utilization bicarbonate ion B
In first step, with two kinds of δ 13C value difference value is added to when cultivating plant in nutrient solution as tagging 1 and tagging 2 greater than 8 ‰ sodium bicarbonate, both cultivate simultaneously, condition of culture is in full accord, difference is two culture experiment, add tagging 1 in a culture fluid, add tagging 2 in another culture fluid.
In second step, cultivate respectively with the culture fluid of two kinds of tagging sodium bicarbonates the plant of being investigated simultaneously, after their length had 4 above true leaves, the first of measuring respectively simultaneously them launched the stable carbon isotope composition δ of leaf fully 13The C value.
In third step, calculate the bicarbonate ion of being investigated plant utilization and account for inorganic carbon source ratio share, be the δ with the sodium bicarbonate of tagging 1 13The C value is as δ C1, by the δ that is investigated plant leaf blade of tagging 1 cultivation 13The C value is as δ T1, the δ of the sodium bicarbonate of tagging 2 13The C value is as δ C2, by the δ that is investigated plant leaf blade of tagging 2 cultivations 13The C value is as δ T2, bring f into B=
Figure 19933DEST_PATH_IMAGE002
, calculate the bicarbonate ion of being investigated plant utilization and account for inorganic carbon source ratio share f B, it is 1-f that the plant utilization Carbon Dioxide in Air accounts for inorganic carbon source ratio share as carbon source B
Advantage of the present invention is as follows:
1) this method can the inorganic carbon source share of quick obtaining plant utilization.
2) this method does not need to obtain the isotope δ of two end members 13The absolute value of C, only need are measured the δ of two isotope-labeled sodium bicarbonates 13Therefore the C value needs step few, calculates simple.
3) this method is carried out two culture experiment under identical experiment condition, and therefore, the data of obtaining the inorganic carbon source share of plant utilization are more reliable.
Embodiment
Embodiments of the invention: it comprises the following steps, and the first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ 13C value difference value is added to respectively in nutrient solution greater than 8 ‰ sodium bicarbonate and is cultivated plant as tagging 1 culture fluid and tagging 2 culture fluids.Other condition of culture are by the investigation condition, as pH, can select to need to investigate the share of utilizing inorganic carbon source of this plant under which kind of pH value.The δ of bicarbonate ion in tagging 1 culture fluid 13The C value is δ C1, the δ of bicarbonate ion in tagging 2 culture fluids 13The C value is δ C2, δ C1With δ C2Difference generally greater than 8 ‰ (PDB).The second, after cultivation has 4 above true leaves to length, measure respectively simultaneously the corresponding stable carbon isotope composition δ that is investigated plant leaf blade that two kinds of isotope-labeled culture fluids are cultivated 13The C value, δ T1And δ T2Value; The 3rd, with δ C1, δ C2, δ T1And δ T2Bring Equation f into B=
Figure 477459DEST_PATH_IMAGE002
, calculate the share f of plant utilization bicarbonate ion B, it is 1-f that the plant utilization Carbon Dioxide in Air accounts for inorganic carbon source ratio share as carbon source B
Detailed implementation process and theing contents are as follows:
First step, selection is added to the sodium bicarbonate reagent of nutrient solution, measures the δ that is added to the sodium bicarbonate in nutrient solution 15The C value is selected two kinds of δ 13C value difference value is added to respectively in nutrient solution as tagging 1 and tagging 2 greater than 8 ‰ sodium bicarbonate, obtains tagging 1 culture fluid and tagging 2 culture fluids; The δ of bicarbonate ion in tagging 1 culture fluid 13The C value is δ C1, the δ of bicarbonate ion in tagging 2 culture fluids 13The C value is δ C2
Second step, the plant that to be investigated is used respectively in tagging 1 culture fluid and tagging 2 culture fluid water plantings simultaneously, after the plant to be planted growth has 4 above true leaves, measure respectively the corresponding quilt of two kinds of taggings cultivations and investigate the δ that plant first is launched leaf fully 15The C value, δ T1And δ T2Value.
Third step is with δ C1, δ C2, δ T1And δ T2Bring Equation f into B=
Figure 291831DEST_PATH_IMAGE002
, calculate the share f of plant utilization bicarbonate ion BThe ability of the airborne carbonic acid gas of plant utilization is 1-f B
Implementation result of the present invention is as follows:
Use respectively δ 13C is mixed with tagging 1 culture fluid and tagging 2 culture fluids for-17.23 ‰ and-6.69 ‰ sodium bicarbonate adds to through in the Hoagland nutrient solution of improveing.Getting Broussonetia papyrifera seed is seeded on the dish of cave.After sprouting, cultivate with tagging 1 culture fluid and tagging 2 culture fluids respectively.The pH of culture fluid is 8.2; After these plants grow to 4 true leaves, measure respectively the δ that these plants first are launched leaf fully 13The C value.Use the inventive method, the paper mulberry that draws utilizes the share f of bicarbonate ion BWith utilize airborne carbonic acid gas and make the share (1-f of carbon source B), as table 1.
Table 1 paper mulberry utilizes the share of bicarbonate ion and utilizes airborne carbonic acid gas to make the share of carbon source
As can be seen from Table 1, when sodium bicarbonate concentration is 5 mM, paper mulberry pH be the bicarbonate ion of 8.2 o'clock to utilize share be 0.17, when sodium bicarbonate concentration is 10 mM, paper mulberry is that the bicarbonate ion of 8.2 o'clock utilizes 0.19 of share at pH, and both results are very close.Illustrate that the method has reliability.Illustrate that also paper mulberry is good karst adaptive plant, have nearly 20% photosynthetic DIC to derive from the bicarbonate ion of soil, to have a higher carbonic anhydrase activity relevant with paper mulberry for this.Under the karst adverse circumstance, the interaction energy of paper mulberry by carbonic anhydrase with bicarbonate ion change into water and carbonic acid gas for plant leaf blade at stomatal closure or the open photosynthesis that carries out when not enough.This is consistent with existing conclusion.Simultaneously, paper mulberry illustrates also that to the share height of bicarbonate ion utilization the karst adaptive plant has strategy preferably to increasing the carbon remittance.

Claims (2)

1. method of utilizing double labelling to obtain the inorganic carbon source share of plant utilization, it is characterized in that: it comprises the following steps: the first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ 13C value difference value is added to respectively as tagging 1 and tagging 2 greater than 8 ‰ sodium bicarbonate and is cultivated plant in nutrient solution; The δ of bicarbonate ion in tagging 1 culture fluid 13The C value is δ C1, the δ of bicarbonate ion in tagging 2 culture fluids 13The C value is δ C2The second, after cultivation has 4 above true leaves to length, measure respectively the corresponding stable carbon isotope composition δ that is investigated plant leaf blade that two kinds of taggings are cultivated 13The value of C, δ T1And δ T2Value; The 3rd, with δ C1, δ C2, δ T1And δ T2Bring equation into , calculate the share f of plant utilization bicarbonate ion B
In first step, with two kinds of δ 13C value difference value is added to when cultivating plant in nutrient solution as tagging 1 and tagging 2 greater than 8 ‰ sodium bicarbonate, both cultivate simultaneously, condition of culture is in full accord, difference is two culture experiment, add tagging 1 in a culture fluid, add tagging 2 in another culture fluid;
In second step, cultivate respectively with the culture fluid of two kinds of tagging sodium bicarbonates the plant of being investigated simultaneously, after their length had 4 above true leaves, the first of measuring respectively simultaneously them launched the stable carbon isotope composition δ of leaf fully 13The C value.
2. the method for utilizing double labelling to obtain the inorganic carbon source share of plant utilization according to claim 1, it is characterized in that: in third step, calculating the bicarbonate ion of being investigated plant utilization and account for inorganic carbon source ratio share, is the δ with the sodium bicarbonate of tagging 1 13The C value is as δ C1, the δ that is investigated plant leaf blade that is cultivated by the culture fluid of tagging 1 13The C value is as δ T1, the δ of the sodium bicarbonate of tagging 2 13The C value is as δ C2, the δ that is investigated plant leaf blade that is cultivated by the culture fluid of tagging 2 13The C value is as δ T2, bring into
Figure DEST_PATH_IMAGE003
, calculate the bicarbonate ion of being investigated plant utilization and account for inorganic carbon source ratio share f B, it is 1-f that the plant utilization Carbon Dioxide in Air accounts for inorganic carbon source ratio share as carbon source B
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* Cited by examiner, † Cited by third party
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CN102792891A (en) * 2012-08-09 2012-11-28 中国科学院地球化学研究所 Method for determining tissue culture seedling autotrophic portion
CN102827916B (en) * 2012-08-09 2014-04-16 中国科学院地球化学研究所 Method for quantifying inorganic carbon approach for microalgae utilization
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CN104142382B (en) * 2014-07-17 2016-02-17 中国农业大学 A kind of isotope stem stalk double-tagging tracing method
CN105067772B (en) * 2015-08-10 2016-09-21 中国科学院地球化学研究所 A kind of method measuring plant gross photosynthesis carbon assimilation ability
CN105181820B (en) * 2015-09-07 2017-06-16 中国科学院地球化学研究所 A kind of determination method of the stable carbon isotope fractionation value during microalgae carton dioxide assimilation
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CN112501237B (en) * 2020-11-12 2022-12-23 中国科学院地球化学研究所 Method for predicting utilization capacity of microalgae bicarbonate
CN113567624B (en) * 2021-07-21 2022-04-22 中国科学院地球化学研究所 Method for quantitatively measuring autotrophic and heterotrophic efficiency of different nitrogen sources of tissue culture seedlings
CN113884621B (en) * 2021-08-16 2022-08-02 中国科学院地球化学研究所 Method for quantitatively absorbing and utilizing bicarbonate by plants
CN114097594A (en) * 2021-11-24 2022-03-01 南开大学 Method for efficiently marking plants by nitrogen isotopes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599218A (en) * 1982-03-01 1986-07-08 Chevron Research Company Capture box for predicting hydrocarbon potential of an earth formation underlying a body of water
CN101015248A (en) * 2006-07-28 2007-08-15 中国科学院亚热带农业生态研究所 Method and device for plants carbon isotope labelling
CN101216385A (en) * 2007-12-26 2008-07-09 中国科学院沈阳应用生态研究所 N15/14 isotopic abundance sample collection method and apparatus for determining plant discharging N2O
CN101926267A (en) * 2010-08-09 2010-12-29 中国科学院地球化学研究所 Method for measuring bicarbonate ion utilizing capability of plant

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599218A (en) * 1982-03-01 1986-07-08 Chevron Research Company Capture box for predicting hydrocarbon potential of an earth formation underlying a body of water
CN101015248A (en) * 2006-07-28 2007-08-15 中国科学院亚热带农业生态研究所 Method and device for plants carbon isotope labelling
CN101216385A (en) * 2007-12-26 2008-07-09 中国科学院沈阳应用生态研究所 N15/14 isotopic abundance sample collection method and apparatus for determining plant discharging N2O
CN101926267A (en) * 2010-08-09 2010-12-29 中国科学院地球化学研究所 Method for measuring bicarbonate ion utilizing capability of plant

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
刘微等.稳定碳同位素技术在土壤_植物系统碳循环中的应用.《应用生态学报》.2008,第19卷(第3期),674-680.
唐艳凌等.稳定氮同位素示踪技术在湿地脱氮研究中的应用进展.《湿地科学》.2008,第6卷(第3期),440-446.
尹云锋等.植物富集13C标记技术的初步研究.《土壤学报》.2010,第47卷(第4期),790-793.
植物富集13C标记技术的初步研究;尹云锋等;《土壤学报》;20100731;第47卷(第4期);790-793 *
稳定氮同位素示踪技术在湿地脱氮研究中的应用进展;唐艳凌等;《湿地科学》;20080930;第6卷(第3期);440-446 *
稳定碳同位素技术在土壤_植物系统碳循环中的应用;刘微等;《应用生态学报》;20080331;第19卷(第3期);674-680 *

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