CN107219334B - A kind of comprehensive titer detection method of young coal degradation solution - Google Patents
A kind of comprehensive titer detection method of young coal degradation solution Download PDFInfo
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Abstract
The invention discloses a kind of comprehensive titer detection methods of young coal degradation solution, detect young coal degradation solution to crop seed sprout and growth course in a variety of representative preferred parameters influence, the integrally-regulated effect for being converted to a kind of comprehensive organism potency by comparative study to characterize degradation solution to crop growth；Relative to chemical analysis, this method is more intuitive, accurately detects the size of integrally-regulated effect of the young coal degradation solution to plant growth and development, and this method stability is good, strong applicability, and standardization level is high；Simultaneously it is easy to operate, the time is short, at low cost, be suitable for large-scale promotion use, filled up the blank of young coal degradation solution activity test method, be its product popularization and application and industrialization development clear away a big obstacle.
Description
Technical field
The present invention relates to a kind of comprehensive titer detection methods of young coal degradation solution, especially relate to a kind of young coal degradation of evaluation
The detection method of the active comprehensive potency of liquid coordinate plant growth, belongs to technical field of biological.
Background technique
Researcher is when carrying out oxidative degradation to the young coal such as lignite, peat with oxidant, it was found that a kind of colourless
Bright distillate, pH is weakly acidic between 2.0 to 2.6, is referred to as young coal degradation solution.Primary Study finds the drop
It solves liquid and is adjusting soil acidity or alkalinity, improve percentage of seedgermination, enhance crop anti-adversity (salt resistance, drought resisting), promote plant growth hair
It educates, increasing crop yield etc. all has the effect of fabulous, can be used as the novel Multifunctional crop conditioner of one kind.It is made
Preparation Method and as plant growth regulator application applied for national inventing patent, and authorized (application number:
201410006333.5).Currently, young coal degradation solution has entered quotient as a kind of significantly novel crop growth regulator that acts on
The product stage, but its evaluation method for adjusting physiologically active of plant is always the bottleneck that it promotes and applies and is commercialized.Till now
Until there are no it is effective for the degradation solution it is active chemistry or biology standardization detection method appearance.On the one hand,
Degradation solution contains the acidic materials of a large amount of small molecules, and polarity is extremely strong, and property is close, it is difficult to use the technologies such as common HPLC, GC
Means carry out separation identification and quantitative analysis to it；On the other hand, for a kind of novel natural active matter, even if realizing
The quantitative analysis of some chemical properties, because its active material base is unknown, it is also difficult to determine which is active material, if tool
It is representative, whether there is synergistic effect etc. between heterogeneity.Furthermore using crop growth degree or yield as detection
The conventional method of index is also unsuitable for the foundation and popularization and application of standardized method because influence factor is more, the period is long, at high cost,
These reasons make the foundation of young coal degradation solution activity measure method face huge challenge.
Further research finds there is very big relevance between the young above-mentioned many activity of coal degradation solution, i.e., logical
The effects of crossing raising percentage of seedgermination, adjusting growth course, enhancing crop anti-adversity (salt resistance, drought resisting) promotes crop on the whole
Growth and development finally increases crop yield.But for the degradation solution sample of separate sources or different diluted concentration, various effects
Between be not to be completely positively correlated, such as there may be certain a samples can increase budding rate, but inhibit radicle stretch
The case where long and reduction seedling weight, the similar different samples of overall activity are strong to the adjusting of plant difference growth parameter(s) in other words
Degree is inconsistent, so single activity index is difficult to accurately evaluate the overall activity of degradation solution.
Summary of the invention
In view of the above problems, the present invention establishes a set of comprehensive titer detection method, i.e., it is young under detection salt stress environment
Influence of the coal degradation solution to representative preferred parameters a variety of in crop seed sprouting and growth course, is converted by comparative study
Biological value characterizes integrally-regulated effect of the degradation solution to crop growth；Relative to chemical analysis, this method can be with
Activity that is more intuitive, accurately characterizing young coal degradation solution coordinate plant growth, makes a breakthrough in terms of its biological detection, fills up
The blank of this new type natural lytic activity detection method of young coal degradation solution.
The present invention provides a kind of comprehensive titer detection method of young coal degradation solution, especially a kind of standardization of biological detection
Method, particular content are as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, is rushed after 10%NaClO sterilizes 8-10min with sterile distilled water
It washes 6-8 times, Chinese cabbage seed immersion 5-6h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is used into constant incubator under identical environment
It is cultivated, diurnal temperature is maintained at 25-30 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z = a×F +
B × P+c × Y, wherein a, b, c are potency index, for Chinese cabbage seed a=2.8, b=3.0, c=4.2, potency positive value
Indicate that facilitation, negative value indicate inhibiting effect；Absolute value is bigger, and the effect for indicating coordinate plant growth is stronger.
The kind of Chinese cabbage described in this detection method is gold zone dish, is the one of Cruciferae Btassica rape kind Chinese cabbage subspecies
A cultivar is Chinese cabbage, and preferably for the seed of gold zone dish as test material, germinating time is short, and germination temperature is wanted
Ask not high, under salt stress germination percentage it is moderate, stablize, farthest reduce detection time, improve method stability and
Applicability.
The sample that this detection method is measured --- young coal degradation solution, the patent of invention for refering in particular to authorize are " a kind of young
Young coal degradation solution, contains described in the production method and application thereof of coal degradation solution " (application number CN201410006333.5)
Justice and preparation method can refer to the content of the patent.
In the method, all test parameters are the optimum condition after optimization, but seed disinfection time, distilled water flushing time
The parameters such as number, seed soaking time, cultivation temperature change not the stability and method for influencing testing result within the scope of defined
Standardization, and allow the small-scale variation of non-key factor that can increase adaptation of methods, reduce cost.
Advantage and innovation of the invention:
(1) first passage detects young coal degradation solution to representative preferred parameter in crop seed sprouting and growth course
Influence and with blank group comparative study, successfully construct the synthesis potency model of young coal degradation solution Activity determination.
(2) relative to chemical analysis, which can more intuitive, accurately detect young coal degradation solution pair
The size of the integrally-regulated effect of plant growth and development.
(3) this method stability is good, strong applicability, and standardization level is high；Simultaneously it is easy to operate, the time is short, at low cost, be suitable for
Large-scale promotion uses, and has filled up the blank of young coal degradation solution activity test method, is the popularization and application and industry of its product
Melt hair one big obstacle of cleaning.
Detailed description of the invention
Fig. 1 is the variation tendency comparison of the comprehensive potency of sample and Chinese cabbage single plant counterpoise (yield)；
Fig. 2 is the regression analysis of sample comprehensive potency and Chinese cabbage single plant counterpoise (yield).
Specific embodiment
Below by embodiment, invention is further described in detail, but the scope of the present invention is not limited in described
Hold.
Embodiment 1
The present embodiment is using Zhaotong County, Yunnan lignite as raw material, by patent " a kind of production method and its use of young coal degradation solution
The method of embodiment 1 prepares young coal degradation solution (sample 1) in (application number CN201410006333.5) on the way ", measures its pH, right
The comprehensive potency of this young coal degradation solution coordinate plant growth activity is detected, and the specific method is as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, rinses 6 with sterile distilled water after 10%NaClO sterilizes 8min
It is secondary, Chinese cabbage seed immersion 5h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is carried out being trained with constant temperature under identical environment
Feeding case is cultivated, and diurnal temperature is maintained at 25 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z= 2.8×F +
3.0×P + 4.2×Y。
After the test, Chinese cabbage seedling was further cultured for 15 days, is transplanted to continued growth in the soil in greenhouse, every 10 days
The young coal degradation solution that the present embodiment that foliage-spray dilutes 100 times is prepared is primary, picks within the 50th day after transplanting, surveys
Ding white ware menu strain counterpoise.
Embodiment 2
The present embodiment is using Yunnan E Shan lignite as raw material, by patent " a kind of production method and its use of young coal degradation solution
The method of embodiment 1 prepares young coal degradation solution (sample 2) in (application number CN201410006333.5) on the way ", measures its pH, right
The comprehensive potency of this young coal degradation solution coordinate plant growth activity is detected, and the specific method is as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, is rinsed after 10%NaClO sterilizes 10min with sterile distilled water
6 times, Chinese cabbage seed immersion 6h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is carried out being trained with constant temperature under identical environment
Feeding case is cultivated, and diurnal temperature is maintained at 30 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z= 2.8×F +
3.0×P + 4.2×Y。
After the test, Chinese cabbage seedling was further cultured for 15 days, is transplanted to continued growth in the soil in greenhouse, every 10 days
The young coal degradation solution that the present embodiment that foliage-spray dilutes 100 times is prepared is primary, picks within the 50th day after transplanting, surveys
Ding white ware menu strain counterpoise.
Embodiment 3
The present embodiment is using Xundian Area, Yunnan lignite as raw material, by patent " a kind of production method and its use of young coal degradation solution
The method of embodiment 1 prepares young coal degradation solution (sample 3) in (application number CN201410006333.5) on the way ", measures its pH, right
The comprehensive potency of this young coal degradation solution coordinate plant growth activity is detected, and the specific method is as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, rinses 6 with sterile distilled water after 10%NaClO sterilizes 9min
It is secondary, Chinese cabbage seed immersion 6h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is carried out being trained with constant temperature under identical environment
Feeding case is cultivated, and diurnal temperature is maintained at 28 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z= 2.8×F +
3.0×P + 4.2×Y。
After the test, Chinese cabbage seedling was further cultured for 15 days, is transplanted to continued growth in the soil in greenhouse, every 10 days
The young coal degradation solution that the present embodiment that foliage-spray dilutes 100 times is prepared is primary, picks within the 50th day after transplanting, surveys
Ding white ware menu strain counterpoise.
Embodiment 4
The present embodiment using Heilungkiang Baoqing lignite as raw material, by patent " production method of young coal degradation solution a kind of and its
The method of embodiment 1 prepares young coal degradation solution (sample 4) in purposes " (application number CN201410006333.5), measures its pH,
The comprehensive potency of this young coal degradation solution coordinate plant growth activity is detected, the specific method is as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, is rinsed after 10%NaClO sterilizes 10min with sterile distilled water
7 times, Chinese cabbage seed immersion 5.5h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is carried out being trained with constant temperature under identical environment
Feeding case is cultivated, and diurnal temperature is maintained at 27 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z= 2.8×F +
3.0×P + 4.2×Y。
After the test, Chinese cabbage seedling was further cultured for 15 days, is transplanted to continued growth in the soil in greenhouse, every 10 days
The young coal degradation solution that the present embodiment that foliage-spray dilutes 100 times is prepared is primary, picks within the 50th day after transplanting, surveys
Ding white ware menu strain counterpoise.
Embodiment 5
The present embodiment using Heilungkiang Baoqing lignite as raw material, by patent " production method of young coal degradation solution a kind of and its
The method of embodiment 2 prepares young coal degradation solution (sample 5) in purposes " (application number CN201410006333.5), measures its pH,
The comprehensive potency of this young coal degradation solution coordinate plant growth activity is detected, the specific method is as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, rinses 8 with sterile distilled water after 10%NaClO sterilizes 8min
It is secondary, Chinese cabbage seed immersion 6h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is carried out being trained with constant temperature under identical environment
Feeding case is cultivated, and diurnal temperature is maintained at 26 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z= 2.8×F +
3.0×P + 4.2×Y。
After the test, Chinese cabbage seedling was further cultured for 15 days, is transplanted to continued growth in the soil in greenhouse, every 10 days
The young coal degradation solution that the present embodiment that foliage-spray dilutes 100 times is prepared is primary, picks within the 50th day after transplanting, surveys
Ding white ware menu strain counterpoise.
Embodiment 6
The present embodiment using Heilungkiang Baoqing lignite as raw material, by patent " production method of young coal degradation solution a kind of and its
The method of embodiment 3 prepares young coal degradation solution (sample 6) in purposes " (application number CN201410006333.5), measures its pH,
The comprehensive potency of this young coal degradation solution coordinate plant growth activity is detected, the specific method is as follows:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, rinses 6 with sterile distilled water after 10%NaClO sterilizes 8min
It is secondary, Chinese cabbage seed immersion 5h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 diameters
It is covered in the culture dish of 1 layer of filter paper for the bottom of 15cm, 80, every ware (i.e. parallel test three times), each 15mL that is added contains
The Hoagland's culture solution of 120mmol/L NaCl, is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts
It is covered in the culture dish of 1 layer of filter paper to the bottom that 3 diameters are 15cm, 80, every ware (i.e. parallel test three times) is respectively added 15
The Hoagland's culture solution of mL NaCl containing 120mmol/L, is denoted as degradation solution group；
(4) the degradation solution group seed of the distilled water group of step (2) and step (3) is carried out being trained with constant temperature under identical environment
Feeding case is cultivated, and diurnal temperature is maintained at 30 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water benefit in culture dish
The moisture that evaporation loses is filled, guarantees the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, calculated after cultivating 60h
Percentage of seedgermination；Radicle length is measured after cultivating 108h；Seedling fresh weight is measured after cultivating 156h；
(6) by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, respectively
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) for calculating young coal degradation solution, finally calculate comprehensive
Potency (Z), F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water
Group radicle length -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z= 2.8×F +
3.0×P + 4.2×Y。
After the test, Chinese cabbage seedling was further cultured for 15 days, is transplanted to continued growth in the soil in greenhouse, every 10 days
The young coal degradation solution that the present embodiment that foliage-spray dilutes 100 times is prepared is primary, picks within the 50th day after transplanting, surveys
Ding white ware menu strain counterpoise.
Embodiment 1-6 respectively degrades to the young coal that the different material place of production and same place of production difference preparation method are prepared
Liquid sample carries out comprehensive bioactivity, while measuring the production for applying the Chinese cabbage maturity period of young coal degradation solution in growth course always
It measures (being indicated with single plant counterpoise), yield is index that is most direct, best embodying sample adjustment effect, and specific test data is detailed in table
1。
The young coal degradation solution of 1 embodiment 1-6 of table integrates the comparison of potency and the influence to Chinese cabbage yield
As seen from the table, comparative example 1-4 is prepared young using the lignite of different sources as raw material with Same Way
Coal degradation solution, pH value are different, to germination potency, radicle elongation potency, seedling quality potency, comprehensive potency, single plant counterpoise this
The influence of a little indexs also has larger difference；Comparative example 4-6, the same lignite place of production, when extracting method difference, degradation solution pH value
It is close, it integrates potency or single plant counterpoise difference is also little, illustrate that the slightly change of extraction conditions has substantially no effect on its activity, together
When also indicate that the stability of the evaluation method is preferable；Comparative example 4 and 5, even if young coal degradation solution is to germination potency, radicle
It is inconsistent to extend potency, the effect tendency of seedling quality potency these three indexs, but when comprehensive potency is close, single plant counterpoise also phase
Closely, the practical significance that different indexs are designed for uniformity to " comprehensive potency " is embodied；It can also be seen that different samples from table
Synthesis potency and single plant counterpoise variation tendency it is almost the same, show that this method is living for evaluating degradation solution coordinate plant growth
The reliability of property is preferable.
The consistency that Fig. 1 more intuitively shows comprehensive potency and single plant counterpoise (yield) variation tendency is preferable；With single plant
Counterpoise (yield) is dependent variable, and comprehensive potency is independent variable, carries out regression analysis to the two, regression equation is shown in that Fig. 2, degree of fitting are
0.956, show that comprehensive potency and the correlation of single plant counterpoise (yield) are preferable, it was demonstrated that the detection method is to integrate potency characterization drop
Solve the active validity and reliability of liquid coordinate plant growth.
Claims (2)
1. a kind of comprehensive titer detection method of young coal degradation solution, which is characterized in that including the following contents:
(1) it takes the Chinese cabbage seed of full seed to be rinsed with water, rinses 6- with sterile distilled water after 10%NaClO sterilizes 8-10min
8 times, Chinese cabbage seed immersion 5-6h is subjected to sensitization with distilled water or the young coal degradation solution of 100 times of dilution；
(2) it takes step (1) same criticize to use the consistent Chinese cabbage seed of distilled water immersion sensitization 240, neatly puts to 3 bottoms and be covered with
In the culture dish of 1 layer of filter paper, the Hoagland's culture of 15mL NaCl containing 120mmol/L is added in each culture dish for 80, every ware
Liquid is denoted as distilled water group；
(3) it separately takes step (1) same criticize to impregnate sensitization consistent Chinese cabbage seed 240 with young coal degradation solution, neatly puts to 3
Bottom is covered in the culture dish of 1 layer of filter paper, 80, every ware, is added 15 mL NaCl's containing 120mmol/L in each culture dish
Hoagland's culture solution is denoted as degradation solution group；
(4) seed of the distilled water group of step (2) and the degradation solution group of step (3) is subjected to constant temperature incubation under identical environment,
Diurnal temperature is maintained at 25-30 DEG C, and the average photoperiod is 12h, and every 12h weight method fills the water supplement evaporation in culture dish and loses
Moisture, guarantee the constant of salinity；
(5) using seed base-root length be more than seed length 1/2 as germination standard, cultivate 60h after calculate seed send out
Bud rate；Radicle length is measured after culture 108h；Seedling fresh weight is measured after cultivating 156h；
(6) it by germination percentage, radicle length and the seedling fresh weight of comparison distilled water group seed and degradation solution group seed, calculates separately
Germination potency (F), radicle elongation potency (P) and the seedling quality potency (Y) of young coal degradation solution finally calculate to obtain comprehensive potency
(Z)；F=(degradation solution group germination percentage/distilled water group germination percentage -1) × 100；P=(degradation solution group radicle length/distilled water group embryo
Root long degree -1) × 100；Y=(degradation solution group seedling fresh weight/distilled water group seedling fresh weight -1) × 100；Z = 2.8×F +3.0
×P + 4.2×Y。
2. the comprehensive titer detection method of young coal degradation solution according to claim 1, which is characterized in that the kind of the Chinese cabbage
It is a cultivar of Cruciferae Btassica rape kind Chinese cabbage subspecies for gold zone dish, is Chinese cabbage.
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