CN103616477B - Method for measuring daily mean stable carbon isotope composition of atmospheric carbon dioxide - Google Patents
Method for measuring daily mean stable carbon isotope composition of atmospheric carbon dioxide Download PDFInfo
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- CN103616477B CN103616477B CN201310566551.XA CN201310566551A CN103616477B CN 103616477 B CN103616477 B CN 103616477B CN 201310566551 A CN201310566551 A CN 201310566551A CN 103616477 B CN103616477 B CN 103616477B
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 title claims abstract description 158
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 104
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 104
- 229910002092 carbon dioxide Inorganic materials 0.000 title claims abstract description 79
- 239000001569 carbon dioxide Substances 0.000 title claims abstract description 77
- 239000000203 mixture Substances 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 20
- 235000015097 nutrients Nutrition 0.000 claims abstract description 110
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 104
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 52
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 52
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 19
- 239000000700 radioactive tracer Substances 0.000 claims abstract description 19
- 230000012010 growth Effects 0.000 claims abstract description 14
- 238000001948 isotopic labelling Methods 0.000 claims description 65
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims description 44
- 238000011534 incubation Methods 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 11
- 238000002474 experimental method Methods 0.000 abstract description 3
- 108700041286 delta Proteins 0.000 abstract 4
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 19
- 230000000694 effects Effects 0.000 description 12
- 241000219198 Brassica Species 0.000 description 5
- 235000003351 Brassica cretica Nutrition 0.000 description 5
- 235000003343 Brassica rupestris Nutrition 0.000 description 5
- 241001233983 Orychophragmus violaceus Species 0.000 description 5
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 238000005194 fractionation Methods 0.000 description 5
- 235000010460 mustard Nutrition 0.000 description 5
- 239000007789 gas Substances 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 2
- 239000002803 fossil fuel Substances 0.000 description 2
- 239000005431 greenhouse gas Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000000155 isotopic effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- QUWBSOKSBWAQER-UHFFFAOYSA-N [C].O=C=O Chemical compound [C].O=C=O QUWBSOKSBWAQER-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 125000005587 carbonate group Chemical group 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for measuring the daily mean stable carbon isotope composition of atmospheric carbon dioxide, which is characterized by comprising the steps of (I) measuring sodium bicarbonate produced by different manufacturers as tracer agents; (II) adding sodium bicarbonate into a nutrient solution respectively, and setting the concentration of sodium bicarbonate in the nutrient solution to 10mM and the pH value to 8.30; (III) cultivating the plants with same growth cycle by use of the prepared solution at the same time, and after 24 hours of cultivation, separately measuring the values of the stable carbon isotope composition delta<13>C in two isotope-labeled nutrition solutions, namely a value of delta 1 and a value of delta 2; (IV) substituting the measured values of delta C1, delta C2, delta 1 and delta 2 into an equation fm=(delta 1-delta 2)/(delta C1-delta C2), and calculating the proportion fB of added sodium bicarbonate to total inorganic carbon in the solution; (V) judging whether the value of fB is less than 0.6, fetching the measured values obtained by an experiment when the value of fB is less than 0.60, substituting the measured values into an equation delta a=(delta 1*deta C2-delta C1*delta2)/(delta 1+delta C2-delta C1-delta 2), and calculating the average value of inorganic carbon delta<13>C entering the culture solution in atmospheric carbon dioxide within the time period delta a; (VI) substituting the calculated delta a into an equation delta Ca=delta a+Delta CO2(air)-HCO3(aq), and calculating the daily mean stable carbon isotope composition delta Ca of atmospheric carbon dioxide.
Description
Technical field
The present invention relates to a kind of method measuring the per day stable carbon isotope composition of atmospheric carbon dioxide, belong to Eco-Environment System monitoring, administer and reparation field.
Background technology
From the industrial revolution till now, CO
2concentration adds 31%.Have definite evidence to show, these increase the burning being mainly derived from fossil fuel in the mankind's activities such as traffic, heating, generating.By CO
2the greenhouse effect increase caused accounts for 2/3rds of the increase of current greenhouse effect.Between apart from modern 10000 to 250 years, CO in air
2concentration highly stable, maintain between 260 ~ 280ppmv.CO in 250 years of past
2concentration increases 370ppmv, and wherein major part growth appears at nearest decades.Factors clearly shows, mankind's activity is the main cause that greenhouse gas concentration increases.Such as, have good consistance between the rate of change that current greenhouse gas concentration increment rate and the mankind discharge, and this is do not occurred in the air history of several thousand.In addition, the variation tendency that the carbon isotope composition in atmospheric carbon dioxide and carbon dioxide distribute in an atmosphere is consistent with the discharge of mankind's activity.
By traffic, heating, carbon isotope composition in the carbon dioxide of the burning release of fossil fuel in the mankind's activities such as generating and biogenic emission, the carbon dioxide carbon isotope composition of carbonate corrosion and atmospheric envelope itself is different, research air carbon isotope composition Changing Pattern to announcement survey region in the past, present and following carbon dioxide variation tendency, on the impact that predict human activity changes FUTURE ENVIRONMENT, to avoid and control has destructive Climate and Environment Variation, for administer and Revegetation of Eco-environment and promotion Global Sustainable Development have great importance.
The method in the past measuring carbon dioxide stable carbon isotope composition in air mainly collects the gas in region to be measured, carries out the mensuration of carbon isotope.This method, due to the complicacy of the gas in region to be measured and changeability in time, is difficult to obtain the atmospheric carbon dioxide stable carbon isotope composition value with provincial characteristics; Can only obtain the value of some time point, and these values are because the complicacy of gas brings error at measurment to a certain degree in air.Therefore, set up the assay method of carbon dioxide stable carbon isotope composition in the air of a kind of energy Representative Region characteristic of field, research whole world change is had great importance.The present invention is exactly based on isotope two-way mark method, utilizes plant that the feature development of bicarbonate ion can be utilized to go out a kind of method measuring the per day stable carbon isotope composition of atmospheric carbon dioxide.
Summary of the invention
The technical problem to be solved in the present invention is, a kind of method utilizing two-way carbon isotope labelling technology quick obtaining atmospheric carbon dioxide average temperature carbon isotope composition is provided, is difficult to obtain the deficiencies such as the atmospheric carbon dioxide stable carbon isotope composition value with provincial characteristics, the value that can only obtain some time point to overcome prior art.
The present invention takes following technical scheme: it comprises the following steps:
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, it joined respectively in nutrient solution, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the solution of isotope labeling 2
13c value is δ
c2;
3rd, by the solution plant that the incubation growth cycle is consistent of preparing above simultaneously, cultivate after 24 hours, measure stable carbon isotope composition δ in two kinds of isotope-labeled nutrient solutions respectively
13c value is δ
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, calculate δ
ain interior air, carbon dioxide enters into the inorganic carbon δ of nutrient solution during this period of time
13the mean value of C;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq), calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
In a first step, first measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰, as the tracer agent of isotope labeling 1 and isotope labeling 2, adds the sodium bicarbonate δ in nutrient solution
13c value difference value is larger, then Carbon Dioxide in Air enters into the easier identification of effect of solution bicarbonate ion, is easy to later experiments data acquisition.
In the second step, joined respectively in nutrient solution by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, the δ of bicarbonate ion in the solution of isotope labeling 1
13c value is δ
c1, the δ of bicarbonate ion in isotope labeling 2 solution
13c value is δ
c2.In this step, the sodium bicarbonate concentration of preparation is 10 mM, pH is 8.30, nutrient solution is made to be ensure sufficient bicarbonate ion under alkaline environment and Carbon Dioxide in Air exchanges, in order to avoid cause measuring error large because the bicarbonate ion in nutrient solution is very few, or bicarbonate ion too much causes the problems such as the too saturated difficulty of bicarbonate ion in solution and carbon dioxide exchange.
In third step, by the nutrient solution plant that incubation growth is consistent of preparing above simultaneously, cultivate after 24 hours, measure stable carbon isotope composition δ in two kinds of isotope-labeled nutrient solutions respectively
13c value is δ
1and δ
2value.In this step, ensure that the plant growth by cultivating is consistent, and will cultivate simultaneously in same environment to be measured.
In the 4th step, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in solution
b.
In the 5th step, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a; F is required in this step
bvalue is less than 0.60, on the one hand to ensure that bicarbonate ion and air carbon dioxide fully exchange, is convenient to late time data estimation, on the other hand, makes carbon dioxide hydrolyses balance towards the future development generating bicarbonate radical, ensures later stage △
cO2 (air)-HCO3(aq)value.
In the 6th step, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq), calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.In this step, there is isotope fractionation because carbon dioxide hydrolyses forms bicarbonate ion, be about 8.5 ‰ in the state of the equilibrium when 25 DEG C, due to absorbing fast of plant, make carbon dioxide hydrolyses balance towards the future development generating bicarbonate radical, now △
cO2 (air)-HCO3(aq)value get 1.1 ‰.Therefore the δ that this experimental calculation goes out
avalue is only the inorganic carbon δ that carbon dioxide in air enters into nutrient solution
13the mean value of C, therefore, atmospheric carbon dioxide per day stable carbon isotope composition δ
cavalue is δ
a+ 1.1 ‰.
Advantage of the present invention is as follows:
1) the per day stable carbon isotope composition of the atmospheric carbon dioxide under this method energy quick obtaining different time varying environment;
2) this method step is few, calculates simple;
3) this method can overcome prior art and is difficult to obtain the deficiencies such as the atmospheric carbon dioxide stable carbon isotope composition value with provincial characteristics, the value that can only obtain some time point;
4) this method absorbs the characteristic of bicarbonate ion fast owing to make use of plant, carbon dioxide hydrolyses is made to become the speed of bicarbonate ion to accelerate, and it is uneven, be convenient to the acquisition that carbon dioxide hydrolyses forms the stable carbon isotope fractionation value of bicarbonate ion on the one hand, ensuring enough bicarbonate ions comes from air carbon dioxide on the other hand, therefore, the data of acquisition are reliable.
Ultimate principle of the present invention is:
The strong fractionation feature of stable carbon isotope is the basis identifying different inorganic carbon source.Occurring in nature carbon has two kinds of stable isotopes:
12c and
13c, their natural average abundance is respectively 98.89% and 1.11%.Stable carbon isotope composition uses δ usually
13c(‰) represent, occurring in nature δ
13c is changed to-90 ‰ ~+20 ‰.The strong fractionation feature of stable carbon isotope is conducive to identifying that different inorganic carbon is originated.Mass balance principle and isotopic dating method and chemometrics method are the bases of the different inorganic carbon source of quantitative judge.
The isotopic dating method of two end members can be expressed as:
δ
i= δ
Ca- f
Biδ
a+ f
Biδ
ci (1)
Here δ
ifor the δ of inorganic carbon in nutrient solution after cultivation plant certain hour
13c value, δ
afor Carbon Dioxide in Air is dissolved into the δ of inorganic carbon in nutrient solution
13c value, δ
cifor the δ of bicarbonate ion in initial incubation liquid
13c value, f
bifor in nutrient solution after cultivation plant certain hour, external source bicarbonate ion accounts for the share in total inorganic carbon (TIC) source in nutrient solution.
Obviously, δ must be known
ci, δ
iand f
biδ can be obtained
a, therefore, the present invention utilizes two kinds of δ
13the sodium bicarbonate that C value differs greatly is added on as mark the plant that in nutrient solution, incubation growth is consistent in environment to be measured simultaneously respectively, estimate that the bicarbonate ion of mark accounts for the ratio of total inorganic carbon (TIC) in solution with two-way mark stable carbon isotope technique, calculate δ thus
a, then according to δ
adraw the per day stable carbon isotope composition of atmospheric carbon dioxide.
for isotope labeling 1, equation (1) is expressed as follows formula:
δ
1= δ
Ca- f
B1 δ
a+ f
B1δ
C1 (2)
Here δ
1for adding the first known δ
13the sodium bicarbonate of C value cultivates δ in the nutrient solution after plant certain hour in nutrient solution
13c value, δ
afor in air in during this period of time, carbon dioxide enters into the inorganic carbon δ of nutrient solution
13the mean value of C, δ
c1for the δ of the first sodium bicarbonate
13c value, f
b1for in nutrient solution after cultivation plant certain hour, the first external source bicarbonate ion added accounts for the share in total inorganic carbon (TIC) source in nutrient solution.
For isotope labeling 2, equation (1) is expressed as follows formula:
δ
2 = δ
Ca- f
B2δ
a+ f
B2δ
C2 (3)
Here δ
2for adding the known δ of the second
13the sodium bicarbonate of C value cultivates δ in the nutrient solution after plant certain hour in nutrient solution
13c value, δ
afor in air in during this period of time, carbon dioxide enters into the inorganic carbon δ of nutrient solution
13the mean value of C, δ
c2for the δ of the second sodium bicarbonate
13c value, f
b2external source bicarbonate ion for the second interpolation in nutrient solution after cultivation plant certain hour accounts for the share in total inorganic carbon (TIC) source in nutrient solution.
(2) and in (3) two equations, f
b=f
b1=f
b2, (2) and (3) simultaneous solution
(4)
Here the f calculated
bvalue for nutrient solution cultivate plant certain hour after the external source bicarbonate ion that adds in nutrient solution account for the share in total inorganic carbon (TIC) source in nutrient solution.
F
bbe worth between 0-1, f
blarger, show that Carbon Dioxide in Air enters into nutrient solution less, carbon dioxide enters into nutrient solution must be fewer, to be then difficult to determine exactly during this period of time carbon dioxide in air and to enter into the inorganic carbon δ of nutrient solution
13mean value (the δ of C
a).F
bless, show that Carbon Dioxide in Air enters into nutrient solution more, carbon dioxide enters into nutrient solution must be more, to be more convenient to determine exactly during this period of time carbon dioxide in air and to enter into the inorganic carbon δ of nutrient solution
13mean value (the δ of C
a).Therefore, we select to cultivate and can utilize the plant of bicarbonate ion fast, allow the carbon dioxide in air more enter into nutrient solution to reaching.By many experiments, determine f
bcritical value be 0.6, work as f
bbe less than 0.6, can above data be brought into equation (5) formula.
(5)
Therefore, can by measuring the δ of the bicarbonate ion of isotope labeling 1
13c value δ
c1with the δ of the bicarbonate ion of isotope labeling 2
13c value δ
c2, the δ in the solution after nutrient solution cultivation plant a period of time that Simultaneously test adds the bicarbonate ion of correspondence markings
13c value, namely determines δ
1and δ
2value, can to calculate during this period of time carbon dioxide in air according to (5) formula and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a.
Add δ again
abe converted into atmospheric carbon dioxide average temperature carbon isotope composition δ
ca.Conversion expression formula is:
δ
Ca=δ
a+ △
CO2(air)- HCO3（aq） (6)
In (6), δ
cafor atmospheric carbon dioxide average temperature carbon isotope composition, △
cO2 (air)-HCO3(aq)for under nonequilibrium condition, by the carbon isotope fractionation value of bicarbonate ion to carbon dioxide.△
cO2 (air)-HCO3(aq)be about 8.5 ‰ in the state of the equilibrium, due to absorbing fast of plant, make carbon dioxide hydrolyses balance always towards the future development generating bicarbonate radical, therefore △
cO2 (air)-HCO3(aq)value get 1.1 ‰.
Embodiment
Example of the present invention: it comprises the following steps:
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, joined respectively in nutrient solution by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution plant that the incubation growth cycle is consistent under environment to be measured of preparing above simultaneously, cultivate after 24 hours, measure stable carbon isotope composition δ in two kinds of isotope-labeled nutrient solutions respectively
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Embodiment 1
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, join in Hoagland nutrient solution respectively by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution prepared above environment to be measured 1 time simultaneously the incubation growth cycle consistent Orychophragmus violaceus, cultivate after 24 hours, measure stable carbon isotope in two kinds of isotope-labeled Hoagland nutrient solutions respectively and form δ
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Embodiment 2
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, join in Hoagland nutrient solution respectively by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution prepared above environment to be measured 2 times simultaneously the incubation growth cycle consistent mustard type rape, cultivate after 24 hours, measure stable carbon isotope in two kinds of isotope-labeled Hoagland nutrient solutions respectively and form δ
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Embodiment 3
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, join in Hoagland nutrient solution respectively by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution prepared above environment to be measured 3 times simultaneously the incubation growth cycle consistent Orychophragmus violaceus, cultivate after 24 hours, measure stable carbon isotope in two kinds of isotope-labeled Hoagland nutrient solutions respectively and form δ
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Embodiment 4
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, join in Hoagland nutrient solution respectively by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution prepared above environment to be measured 4 times simultaneously the incubation growth cycle consistent Orychophragmus violaceus, cultivate after 24 hours, measure stable carbon isotope in two kinds of isotope-labeled Hoagland nutrient solutions respectively and form δ
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Embodiment 5
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, join in Hoagland nutrient solution respectively by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution prepared above environment to be measured 5 times simultaneously the incubation growth cycle consistent mustard type rape, cultivate after 24 hours, measure stable carbon isotope in two kinds of isotope-labeled Hoagland nutrient solutions respectively and form δ
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Embodiment 6
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, join in Hoagland nutrient solution respectively by the tracer agent of isotope labeling 1 and isotope labeling 2, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the nutrient solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the nutrient solution of isotope labeling 2
13c value is δ
c2;
3rd, by the nutrient solution prepared above environment to be measured 6 times simultaneously the incubation growth cycle consistent mustard type rape, cultivate after 24 hours, measure stable carbon isotope in two kinds of isotope-labeled Hoagland nutrient solutions respectively and form δ
13c value, is designated as δ respectively
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in nutrient solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, to calculate during this period of time carbon dioxide in air and enter into the inorganic carbon δ of nutrient solution
13the mean value δ of C
a;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq)i.e. δ
ca=δ
a+ 1.1 ‰., calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
Implementation result of the present invention is as follows:
Use δ respectively
13c adds in the Hoagland nutrient solution of improvement for the sodium bicarbonate of-28.87 ‰ and-1.53 ‰ (PDB), is mixed with isotope labeling 1 nutrient solution and isotope labeling 2 nutrient solution.Get Orychophragmus violaceus and mustard type rape planting seed coils to cave, after two Plants are sprouted and grown to 4 true leaves, use isotope labeling 1 nutrient solution and isotope labeling 2 nutrient solution, the Orychophragmus violaceus that incubation growth is consistent and mustard type rape respectively.Cultivate after 24 hours, respectively the carbon isotope δ of the nutrient solution that mensuration two kinds of isotope labelings are corresponding
13c value.The share f in total inorganic carbon (TIC) source in nutrient solution is accounted for the bicarbonate ion that the inventive method investigation cultivation plant was added in nutrient solution after 24 hours
b, finally calculate atmospheric carbon dioxide per day stable carbon isotope composition δ
ca, as (table 1) shown in following table.
As can be seen from Table 1,6 difference environment to be measured, atmospheric carbon dioxide per day stable carbon isotope composition is obviously different, and the impact that environment 1 to be measured is subject to mankind's activity is minimum, therefore, the per day stable carbon isotope composition of its atmospheric carbon dioxide is close to Atmospheric CO
2carbon isotope δ
13the mean value (≈-8 ‰) of C, the impact that environment 2 to be measured is subject to mankind's activity is greater than environment 1 to be measured and is less than environment 3,4,5,6 to be measured, and therefore the per day stable carbon isotope composition of its atmospheric carbon dioxide is less than Atmospheric CO
2carbon isotope δ
13the mean value (≈-8 ‰) of C is-11.30 ‰.Environment 3 to be measured and environment to be measured 4 are same environment, and the atmospheric carbon dioxide measured per day stable carbon isotope composition difference is little, is respectively-14.80 ‰ and-15.43 ‰; Environment 5 to be measured and environment to be measured 6 are also same environment, and the atmospheric carbon dioxide measured per day stable carbon isotope composition value is very close, is respectively-14.59 ‰ and-14.55 ‰.And environment 3,4,5,6 to be measured is comparatively large by the effect of human activity, the breathing of the mankind causes their atmospheric carbon dioxide per day stable carbon isotope composition value comparatively Atmospheric CO
2carbon isotope δ
13the mean value of C is more partially negative.These results tally with the actual situation.
Can find out from above data, under utilizing the present invention to obtain varying environment, the per day stable carbon isotope composition of atmospheric carbon dioxide has reliability.The present invention establishes a kind of effective method to obtain the per day stable carbon isotope composition of atmospheric carbon dioxide, and its experimentation is simple, and under varying environment being detected, atmospheric carbon dioxide stable carbon isotope composition changes, convenient quick again.
Claims (1)
1. measure a method for the per day stable carbon isotope composition of atmospheric carbon dioxide, it is characterized in that:
The first, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13the sodium bicarbonate that C value difference value is greater than 10 ‰ is as the tracer agent of isotope labeling 1 and isotope labeling 2;
The second, it joined respectively in nutrient solution, in nutrient solution, sodium bicarbonate concentration is set to 10 mM, and pH is 8.30, bicarbonate ion δ in the solution of isotope labeling 1
13c value is δ
c1, bicarbonate ion δ in the solution of isotope labeling 2
13c value is δ
c2;
3rd, by the solution plant that the incubation growth cycle is consistent of preparing above simultaneously, cultivate after 24 hours, measure stable carbon isotope composition δ in two kinds of isotope-labeled nutrient solutions respectively
13c value is δ
1and δ
2value;
4th, by the δ recorded
c1, δ
c2, δ
1and δ
2value brings equation into
, calculate the share f that the sodium bicarbonate added accounts for total inorganic carbon (TIC) in solution
b;
5th, judge f
bwhether value is less than 0.6, gets f
btest each measured value obtained when value is less than 0.60, bring equation into
, calculate δ
ain interior air, carbon dioxide enters into the inorganic carbon δ of nutrient solution during this period of time
13the mean value of C;
6th, by the δ calculated
avalue brings equation δ into
ca=δ
a+ △
cO2 (air)-HCO3(aq), △ CO
2(air)-HCO
3(aq) value gets 1.1 ‰, calculates atmospheric carbon dioxide per day stable carbon isotope composition δ
ca.
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