CN108319820B - Method for obtaining utilization share of plant bicarbonate in field habitat - Google Patents
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Abstract
The invention discloses a method for obtaining the utilization share of plant bicarbonate in a field habitat, which belongs to the field of crop information detection technology and ecological environment treatment, and comprises the steps of obtaining the concentration data of carbon dioxide in the extracellular atmosphere and the concentration data of carbon dioxide among cells by using a portable photosynthetic apparatus, simultaneously determining the fractionation values of carbon dioxide in the air and stable carbon isotopes of the bicarbonate of soil of plants with different seedling ages and different growth periods in different environments, and combining the composition of the stable carbon isotopes of the carbon dioxide in the atmosphere and the composition of the stable carbon isotopes of the bicarbonate of the rhizosphere soil of the plants; and acquiring instant information of the utilization shares of the bicarbonate of the plants with different seedling ages and different growth periods under different environments according to the double-end isotope mixing model. The method not only can quickly, conveniently and accurately obtain the utilization share of the atmospheric carbon dioxide of the plants with different seedling ages and different growth periods in the field habitat in real time, but also can obtain the utilization share of the plant bicarbonate in the field habitat.
Description
Technical Field
The invention relates to the field of crop information detection technology and ecological environment management, in particular to a method for obtaining the use share of plant bicarbonate in a field habitat, which can be used for measuring the use information of the plant bicarbonate of different seedling ages and different growth periods in real time without fussy plant culture, and provides scientific basis for screening karst suitable plants.
Background
In recent years, numerous experiments have demonstrated that plants can not only photosynthesize using atmospheric carbon dioxide as a substrate, but also can photosynthesize using soil-derived bicarbonate. Especially in karst limestone areas with high concentrations of bicarbonate, the inorganic carbon assimilation capacity of plants was determined only with a photosynthetic apparatus based on the determination of the atmospheric carbon dioxide flux, severely underestimating the productivity of plants in karst areas.
Different plants have different bicarbonate utilization portions under the same condition, and the bicarbonate utilization portions of the same plant are greatly different under different environments. Although some methods can determine the bicarbonate utilization capacity of plants cultivated in both field and laboratory, the methods are difficult to determine the bicarbonate utilization information of plants with different seedling ages and different growth periods in real time under different environments, so that the method can accurately determine the bicarbonate utilization shares of plants with different seedling ages and different growth periods under different environments, and has an important role in correctly evaluating the productivity of the plants, screening karst suitable plant varieties with high productivity and using karst suitable plants to control and restore the fragile karst ecological environment.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for obtaining the utilization share of plant bicarbonate in a field habitat, which can be used for measuring the utilization shares of plant bicarbonate in different seedling ages and different growth periods in real time without fussy plant culture and overcomes the defect that the prior art cannot obtain the utilization information of the plant bicarbonate in batches.
The invention adopts the following technical scheme: it comprises the following steps:
step one, selecting a second unfolded leaf blade on a new branch of a plant growing in a investigated environment, and respectively measuring 9 a.m. by using a portable photosynthetic apparatus in a clear weather: 00-10: a photosynthetic parameter of 00; the leaves of the second unfolded leaves on the new plant branches are taken as the first completely unfolded leaves and the like according to the principle from top to bottom by taking the leaves which are just completely developed and completely unfolded on the new branches as the first completely unfolded leaves; a second fully expanded leaf and a third fully expanded leaf;
step two, acquiring data of extracellular atmospheric carbon dioxide concentration Ca and intercellular carbon dioxide concentration Ci from the portable photosynthetic apparatus, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaf to the extracellular atmospheric carbon dioxide concentration Ca, wherein the calculation method comprises the following steps:
selecting the leaves of the first unfolded leaf on the new branch of the plant growing in the investigated environment, and measuring the stable carbon isotope composition delta of the plant leaves by using a conventional method13The value of C, is noted as deltaT；
Step four, acquiring the stable carbon isotope composition delta of the carbon dioxide in the atmosphere of the investigated environment according to a conventional method13Average value of C, denoted as δa；
Step five, measuring the stable carbon isotope composition delta of the rhizosphere soil bicarbonate of the plants growing in the investigated environment according to a conventional method13The value of C, is noted as deltab；
Step six, according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca; calculating a stable carbon isotope fractionation value delta a of a plant leaf growing under a investigated environment for assimilating carbon dioxide; the calculation method comprises the following steps: Δ a is 4.4 ‰ + (D-4.4 ‰) f, wherein D is fractionation of inorganic carbon during rubisco carboxylation, and is different from plant to plant in a range of 27 ‰ -29 ‰;
seventhly, calculating a stable carbon isotope fractionation value delta b of the plant leaf assimilating bicarbonate growing in the investigated environment according to the stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide growing in the investigated environment; the calculation method comprises the following steps: delta b ═ delta a-4.4 ‰ -9.9 ‰;
step eight, according to the stable carbon isotope composition delta of the plant leaves growing under the investigated environment13Value delta of CTCarbon dioxide stable carbon isotope composition delta of the atmosphere to be investigated13Average value delta of CaStable carbon isotope composition delta of rhizosphere soil bicarbonate of plants grown in the investigated environment13Value delta of Cb(ii) a The stable carbon isotope fraction value Delta a of the plant leaf assimilating carbon dioxide grown in the examined environment and the stable carbon isotope fraction value Delta b of the plant leaf assimilating bicarbonate grown in the examined environment were calculated to calculate the plant bicarbonate utilization fraction fbThe calculation method comprises the following steps:
the basic principle of the invention is as follows:
the strong fractionation characteristics of stable carbon isotopes are the basis for identifying different inorganic carbon sources in plants. Carbon elements in nature have two stable isotopes:12c and13c, their natural average abundances are 98.89% and 1.11%, respectively. The composition of the stable carbon isotope is usually delta13C (‰) represents delta in nature13The change of C is-90 to +20 per mill. The strong fractionation characteristics of stable carbon isotopes are beneficial for identifying different inorganic carbon sources in plants. The mass balance principle, the isotope mixing model and the chemometric method are the basis for quantitatively identifying different inorganic carbon sources in the plant body.
The two-end-member isotope mixture model can be expressed as:
δT＝δA-fbδA+fbδB(1)
where delta isTDelta of leaf of the plant to be investigated13C value, δADelta of leaf for the assumption that plants utilize atmospheric carbon dioxide as sole carbon source13C value, δBDelta of leaf when the plant is assumed to fully utilize bicarbonate from the root as the sole carbon source13C value, fbThe use of exogenously added bicarbonate ions for the study plants accounts for the share of the total inorganic carbon source utilized by the plants.
Plant in-line C3When the method is used, the fractionation Delta a of the carbon dioxide in the air by the blades satisfies the following relation (2).
Δa＝a+(D-a)(Ci/Ca) (2)
In the formula (2), Ci is intercellular carbon dioxide concentration, Ca is carbon dioxide concentration in the air, a is the fractionation of inorganic carbon under the action of pore diffusion, and the value is 4.4 per mill, D is the fractionation of inorganic carbon under the action of Rubsico carboxylation, and the range is 27 per mill to 29 per mill according to different values of plants; order to
Substituting the known parameters into the formula (2) to obtain:
Δa＝4.4‰+(D-4.4‰)f (3)
as shown in formula (3), C3The fractionation value of inorganic carbon when plants assimilate carbon dioxide in the air is proportional to the intercellular carbon dioxide concentration. Transforming the formula (3) into a formula (4) to obtain:
δA＝δa-Δa (4)
in the formula (4), δADelta of leaf for the assumption that plants utilize atmospheric carbon dioxide as sole carbon source13C value, δaThe average value of carbon dioxide stable carbon isotopes in air.
The inorganic carbon fraction when carbon dioxide is hydrolyzed into bicarbonate ions is-9.9 ‰, and the bicarbonate ions used by the plant come from the root, and there is no isotope diffusion fraction as occurs when carbon dioxide gas enters the stomata, so that the leaf fraction Δ b of the inorganic carbon of bicarbonate from the root satisfies the following relationship (5).
Δb＝Δa-4.4‰-9.9‰ (5)
The stable carbon isotope fractionation value delta b of plant leaf assimilation bicarbonate can be converted into stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of CbDelta from leaves when plants are fully utilizing bicarbonate from roots as sole carbon source13C value deltaBSo that expression (5) can be changed to expression (6):
δB＝δb-Δb (6)
by substituting expressions (4) and (6) into expression (1), it is possible to obtain:
obtaining intercellular carbon dioxide concentration Ci, carbon dioxide concentration Ca in air, average value delta a of stable carbon isotopes and stable carbon isotope composition delta of plant leaves13Value delta of CTStable carbon isotope composition of plant rhizosphere soil bicarbonate [ delta ]13Value delta of CbCan calculate the plant bicarbonateSalt utilization fraction fb。
The invention has the following advantages:
the invention measures the fractionation value of carbon dioxide of air and stable carbon isotope of bicarbonate of soil by plants of different seedling ages and different growth periods under different environments in real time, combines the composition of stable carbon isotope of carbon dioxide in atmosphere and the composition of stable carbon isotope of bicarbonate of rhizosphere soil of plants; and acquiring instant information of the utilization shares of the bicarbonate of the plants with different seedling ages and different growth periods under different environments according to the double-end isotope mixing model.
1) The method not only can obtain the stable carbon isotope fractionation value of the plant under the field habitat by utilizing the atmospheric carbon dioxide, but also can obtain the stable carbon isotope fractionation value of the plant under the field habitat by utilizing the bicarbonate from the soil.
2) The method can obtain the utilization share of the plant in the field habitat on the atmospheric carbon dioxide, and also can obtain the utilization share of the plant in the field habitat on the bicarbonate.
3) The method can be used for rapidly, conveniently, accurately and real-timely measuring the utilization portions of the plant bicarbonate in different seedling ages and different growth periods.
4) The invention not only omits the complicated process of cultivating the plant, but also can obtain the utilization information of the plant bicarbonate in batches
5) The invention can also provide a basis for the rapid screening of karst probiotics plants and provide a scientific method for the environmental adaptability research of plants.
Detailed Description
The embodiment of the invention comprises the following steps: which comprises the following steps of,
selecting a second unfolded leaf blade on a new branch of a plant growing in a investigated environment, and respectively measuring 9 a.m. by using a portable photosynthetic instrument in a clear weather: 00-10: a photosynthetic parameter of 00; the leaves of the second unfolded leaves on the new plant branches are taken as the first completely unfolded leaves and the like according to the principle from top to bottom by taking the leaves which are just completely developed and completely unfolded on the new branches as the first completely unfolded leaves; a second fully expanded leaf and a third fully expanded leaf;
step two, acquiring data of extracellular atmospheric carbon dioxide concentration Ca and intercellular carbon dioxide concentration Ci from the portable photosynthetic apparatus, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaf to the extracellular atmospheric carbon dioxide concentration Ca, wherein the calculation method comprises the following steps:
selecting the leaves of the first unfolded leaf on the new branch of the plant growing in the investigated environment, and measuring the stable carbon isotope composition delta of the plant leaves by using a conventional method13The value of C, is noted as deltaT；
Step four, acquiring the stable carbon isotope composition delta of the carbon dioxide in the atmosphere of the investigated environment according to a conventional method13Average value of C, denoted as δa；
Step five, measuring the stable carbon isotope composition delta of the rhizosphere soil bicarbonate of the plants growing in the investigated environment according to a conventional method13The value of C, is noted as deltab；
Step six, according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca; calculating a stable carbon isotope fractionation value delta a of a plant leaf growing under a investigated environment for assimilating carbon dioxide; the calculation method comprises the following steps: Δ a is 4.4 ‰ + (D-4.4 ‰) f, wherein D is fractionation of inorganic carbon during rubisco carboxylation, and is different from plant to plant in a range of 27 ‰ -29 ‰;
seventhly, calculating a stable carbon isotope fractionation value delta b of the plant leaf assimilating bicarbonate growing in the investigated environment according to the stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide growing in the investigated environment; the calculation method comprises the following steps: delta b ═ delta a-4.4 ‰ -9.9 ‰;
step eight, according to the stable carbon isotope composition delta of the plant leaves growing under the investigated environment13Value delta of CTCarbon dioxide stable carbon isotope composition delta of the atmosphere to be investigated13Average value delta of CaGrowing in the environment under investigationThe stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of Cb(ii) a The stable carbon isotope fraction value Delta a of the plant leaf assimilating carbon dioxide grown in the examined environment and the stable carbon isotope fraction value Delta b of the plant leaf assimilating bicarbonate grown in the examined environment were calculated to calculate the plant bicarbonate utilization fraction fbThe calculation method comprises the following steps:
the detailed implementation process and contents are as follows:
1) determination of the utilization share of the camptothecin bicarbonate in three regions of the general Maqiyue month in Guiyang city:
camptotheca acuminata in three regions of July growing in general mountain of Guiyang city was used as a study object, and under a clear weather, the measurement of 9 am was performed by using a portable photosynthetic apparatus: 00-10: 00 a photosynthetic parameter of a leaf of a second expanded leaf on a new branch of the plant; reading the data of the extracellular atmospheric carbon dioxide concentration Ca and the intercellular carbon dioxide concentration Ci from the portable photosynthetic apparatus, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaf to the extracellular atmospheric carbon dioxide concentration Ca, as shown in Table 1; simultaneously determining the stable carbon isotope composition delta of the leaves of the first unfolded leaf on the new branch of the plant13Value delta of CTAs in Table 1; simultaneously obtaining the stable carbon dioxide isotope composition delta of the atmosphere carbon dioxide of the investigated environment13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of CbAs in Table 1; according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca; calculating stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide, as shown in table 2; the calculation method comprises the following steps: Δ a is 4.4 ‰ + (D-4.4 ‰) f, wherein D is 27 ‰; subsequently, the stable carbon isotope fractionation value Δ b of the assimilation of bicarbonate by Camptotheca acuminate leaves was calculated, as shown in Table 2; finally, the composition of the stable carbon isotope of the leaves of Camptotheca acuminate is delta13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaRhizosphere soilStable carbon isotope composition delta of bicarbonate13Value delta of CbThe stable carbon isotope fractionation value delta a of the camptotheca acuminate leaves assimilating carbon dioxide and the stable carbon isotope fractionation value delta b of the camptotheca acuminate leaves assimilating bicarbonate are calculated, and the utilization portion f of the plant bicarbonate is calculatedbSee table 2.
Table 1 shows the ratio f of intercellular carbon dioxide concentration Ci of leaves of Camptotheca acuminata in three regions of Majorana general and Heptaculum, Guiyang city, to extracellular atmospheric carbon dioxide concentration Ca, and the stable carbon isotope composition delta of leaves of the first unfolded leaf on the new branch13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of Cb
| Sample plot | Plant number | f | δa‰ | δT‰ | δb‰ |
| A region | XS-1 | 0.61 | -11.98 | -28.21 | -9.19 |
| A region | XS-2 | 0.66 | -11.98 | -29.44 | -10.00 |
| A region | XS-3 | 0.76 | -11.98 | -30.19 | -10.76 |
| A region | XS-4 | 0.69 | -11.98 | -31.06 | -6.53 |
| Two zones | XS-5 | 0.67 | -11.98 | -29.34 | -9.00 |
| Two zones | XS-6 | 0.73 | -11.98 | -30.81 | -9.12 |
| Two zones | XS-7 | 0.77 | -11.98 | -31.72 | -8.89 |
| Two zones | XS-8 | 0.71 | -11.98 | -30.05 | -5.81 |
| Three zones | XS-9 | 0.75 | -11.98 | -30.50 | -6.55 |
| Three zones | XS-10 | 0.72 | -11.98 | -28.81 | -8.33 |
| Three zones | XS-11 | 0.68 | -11.98 | -29.08 | -8.23 |
| Three zones | XS-12 | 0.74 | -11.98 | -30.53 | -9.68 |
Table 2 shows the stable carbon isotope fraction value Δ a of leaf of Camptotheca acuminata assimilating carbon dioxide, the stable carbon isotope fraction value Δ b of leaf of Camptotheca acuminata assimilating carbon dioxide, and the utilization fraction f of bicarbonateb
2) Determination of utilization share of regionalized aromatic bicarbonate in three months of Maqiyue, general, Guiyang city:
three regional incense in July of general mountain growing in Guiyang city was used as a research object, and under a clear weather, the fragrance in July of Mount general, 9 am was measured by using a portable photosynthetic apparatus: 00-10: 00 a photosynthetic parameter of a leaf of a second expanded leaf on a new branch of the plant; reading the data of the extracellular atmospheric carbon dioxide concentration Ca and the intercellular carbon dioxide concentration Ci from the portable photosynthesizer, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca, as shown in Table 3; simultaneously determining the stable carbon isotope composition delta of the leaves of the first unfolded leaf on the new branch of the plant13Value delta of CTAs in Table 3; simultaneously obtaining the stable carbon dioxide isotope composition delta of the atmosphere carbon dioxide of the investigated environment13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of CbAs in Table 3; according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca; calculating stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide, as shown in table 4; the calculation method comprises the following steps: Δ a is 4.4 ‰ + (D-4.4 ‰) f, wherein D is 29 ‰; subsequently, the stable carbon isotope fractionation value Δ b of the assimilation bicarbonate of the leaves of the incense was calculated, as shown in table 4; finally, theAccording to the stable carbon isotope composition delta of the leaves of the incense13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaStable carbon isotope composition delta of bicarbonate of rhizosphere soil13Value delta of CbThe stable carbon isotope fractionation value delta a of the leaf of the incense for assimilating carbon dioxide and the stable carbon isotope fractionation value delta b of the leaf of the incense for assimilating bicarbonate are calculated, and the utilization portion f of the plant bicarbonate is calculatedbSee table 4.
Table 3 shows the ratio f of intercellular carbon dioxide concentration Ci of leaves of Ganshima in Guiyang city, general and Juyle, to extracellular atmospheric carbon dioxide concentration Ca, and the stable carbon isotope composition delta of leaves of the first expanded leaf on the new branch13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of Cb
Table 4 shows the stable carbon isotope fractionation value Δ a for assimilating carbon dioxide, the stable carbon isotope fractionation value Δ b for assimilating bicarbonate, and the utilization fraction f of bicarbonateb
| Sample plot | Plant number | Δa‰ | Δb‰ | fb％ |
| A region | HX-1 | 17.44 | 3.14 | 4.2 |
| A region | HX-2 | 18.67 | 4.37 | 10.0 |
| A region | HX-3 | 18.67 | 4.37 | 9.1 |
| Two zones | HX-4 | 20.64 | 6.34 | 16.5 |
| Two zones | HX-5 | 20.39 | 6.09 | 9.8 |
| Two zones | HX-6 | 20.39 | 6.09 | 19.0 |
| Two zones | HX-7 | 20.14 | 5.84 | 25.9 |
| Two zones | HX-8 | 20.88 | 6.58 | 21.8 |
| Three zones | HX-9 | 18.18 | 3.88 | 10.2 |
| Three zones | HX-10 | 17.93 | 3.63 | 14.3 |
| Three zones | HX-11 | 18.42 | 4.12 | 21.4 |
| Three zones | HX-12 | 19.41 | 5.11 | 21.8 |
3) Determination of utilization share of camptotheca bicarbonate in three regions of May, August, general, Guiyang city:
taking three regional camptotheca acuminata growing in the general mountain of Guiyang city in August as a research object, under sunny weather, respectively measuring 9 am by using a portable photosynthetic apparatus: 00-10: 00 a photosynthetic parameter of a leaf of a second expanded leaf on a new branch of the plant; reading the data of the extracellular atmospheric carbon dioxide concentration Ca and the intercellular carbon dioxide concentration Ci from the portable photosynthetic apparatus, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaf to the extracellular atmospheric carbon dioxide concentration Ca, as shown in Table 5; simultaneously determining the stable carbon isotope composition delta of the leaves of the first unfolded leaf on the new branch of the plant13Value delta of CTAs in Table 5; simultaneously obtaining the stable carbon dioxide isotope composition delta of the atmosphere carbon dioxide of the investigated environment13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of CbAs in Table 5; according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca; calculating stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide, as shown in table 6; the calculation method comprises the following steps: Δ a ═ 4.4 ‰ +) D-4.4 ‰) f, where D takes the value of 29 ‰; subsequently, the stable carbon isotope fractionation value Δ b of the assimilation bicarbonate of Camptotheca acuminate leaves was calculated, as shown in Table 6; finally, the composition of the stable carbon isotope of the leaves of Camptotheca acuminate is delta13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaStable carbon isotope composition delta of bicarbonate of rhizosphere soil13Value delta of CbThe stable carbon isotope fractionation value delta a of the camptotheca acuminate leaves assimilating carbon dioxide and the stable carbon isotope fractionation value delta b of the camptotheca acuminate leaves assimilating bicarbonate are calculated, and the utilization portion f of the plant bicarbonate is calculatedbSee table 6.
TABLE 5 ratio f of intercellular carbon dioxide concentration Ci of leaf of Camptotheca acuminata in August, June, Guiyang city to extracellular atmospheric carbon dioxide concentration Ca, and stable carbon of leaf of first developed leaf on new branchIsotopic composition delta13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of Cb
| Sample plot | Plant number | f | δa‰ | δT‰ | δb‰ |
| A region | XS-1 | 0.62 | -11.98 | -29.34 | -8.69 |
| A region | XS-2 | 0.60 | -11.98 | -28.77 | -8.62 |
| A region | XS-3 | 0.66 | -11.98 | -28.13 | -9.11 |
| A region | XS-4 | 0.54 | -11.98 | -28.25 | -9.04 |
| A region | XS-5 | 0.68 | -11.98 | -30.62 | -6.18 |
| Two zones | XS-6 | 0.56 | -11.98 | -29.23 | -10.93 |
| Two zones | XS-7 | 0.74 | -11.98 | -30.31 | -10.07 |
| Two zones | XS-8 | 0.65 | -11.98 | -28.76 | -8.35 |
| Two zones | XS-9 | 0.61 | -11.98 | -26.75 | -7.98 |
| Three zones | XS-10 | 0.63 | -11.98 | -27.72 | -9.65 |
| Three zones | XS-11 | 0.67 | -11.98 | -30.07 | -8.49 |
| Three zones | XS-12 | 0.80 | -11.98 | -30.64 | -10.24 |
Table 6 shows the stable carbon isotope fraction value Δ a of leaf assimilation of Camptotheca acuminata in three regions of May, August, of Guiyang city, the stable carbon isotope fraction value Δ b of assimilation of bicarbonate, and the bicarbonate utilization fraction fb
| Sample plot | Plant number | Δa‰ | Δb‰ | fb％ |
| A region | XS-1 | 19.65 | 5.35 | 13.0 |
| A region | XS-2 | 19.16 | 4.86 | 13.4 |
| A region | XS-3 | 20.64 | 6.34 | 26.1 |
| A region | XS-4 | 17.68 | 3.38 | 8.2 |
| A region | XS-5 | 21.13 | 6.83 | 12.4 |
| Two zones | XS-6 | 18.18 | 3.88 | 6.0 |
| Two zones | XS-7 | 22.60 | 8.30 | 26.3 |
| Two zones | XS-8 | 20.39 | 6.09 | 20.1 |
| Two zones | XS-9 | 19.41 | 5.11 | 25.3 |
| Three zones | XS-10 | 19.90 | 5.60 | 25.0 |
| Three zones | XS-11 | 20.88 | 6.58 | 15.7 |
| Three zones | XS-12 | 24.08 | 9.78 | 33.8 |
4) Determination of utilization share of three regionalized aromatic bicarbonate in august of general of Guiyang city:
three regional incense in august growing in general mountain of Guiyang city are used as research objects, and under clear weather, the fragrance in August is measured by a portable photosynthetic apparatus at 9 am: 00-10: 00 a photosynthetic parameter of a leaf of a second expanded leaf on a new branch of the plant; reading the data of the extracellular atmospheric carbon dioxide concentration Ca and the intercellular carbon dioxide concentration Ci from the portable photosynthesizer, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca, as shown in Table 7; simultaneously determining the stable carbon isotope composition delta of the leaves of the first unfolded leaf on the new branch of the plant13Value delta of CTAs in Table 7; simultaneously obtaining the stable carbon dioxide isotope composition delta of the atmosphere carbon dioxide of the investigated environment13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of CbAs in Table 7; according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca; calculating stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide, as shown in table 8; the calculation method comprises the following steps: Δ a is 4.4 ‰ + (D-4.4 ‰) f, wherein D is 27 ‰; subsequently, the stable carbon isotope fractionation value Δ b of the assimilation bicarbonate of the leaves of the incense was calculated, as shown in table 8; finally, the stable carbon isotope composition delta of the leaves of the incense is determined13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaRhizosphereStable carbon isotope composition delta of soil bicarbonate13Value delta of CbThe stable carbon isotope fractionation value delta a of the leaf of the incense for assimilating carbon dioxide and the stable carbon isotope fractionation value delta b of the leaf of the incense for assimilating bicarbonate are calculated, and the utilization portion f of the plant bicarbonate is calculatedbSee table 8.
Table 7 shows the ratio f of intercellular carbon dioxide concentration Ci of leaves aromatized in three regions of August of general Shanxi in Guiyang city to extracellular atmospheric carbon dioxide concentration Ca, and the stable carbon isotope composition delta of leaves of the first expanded leaf on the new branch13Value delta of CTCarbon dioxide stable carbon isotope composition delta of atmosphere13Average value delta of CaAnd the stable carbon isotope composition delta of plant rhizosphere soil bicarbonate13Value delta of Cb
| Sample plot | Plant number | f | δa‰ | δT‰ | δb‰ |
| A region | HX-1 | 0.60 | -11.98 | -28.70 | -6.94 |
| A region | HX-2 | 0.65 | -11.98 | -26.25 | -10.49 |
| A region | HX-3 | 0.58 | -11.98 | -28.49 | -7.98 |
| Two zones | HX-4 | 0.59 | -11.98 | -28.28 | -10.10 |
| Two zones | HX-5 | 0.59 | -11.98 | -28.46 | -10.07 |
| Two zones | HX-6 | 0.64 | -11.98 | -29.08 | -11.49 |
| Two zones | HX-7 | 0.72 | -11.98 | -30.60 | -8.35 |
| Two zones | HX-8 | 0.68 | -11.98 | -29.46 | -7.98 |
| Two zones | HX-9 | 0.69 | -11.98 | -28.58 | -6.01 |
| Three zones | HX-10 | 0.68 | -11.98 | -28.92 | -7.53 |
| Three zones | HX-11 | 0.65 | -11.98 | -27.80 | -9.04 |
| Three zones | HX-12 | 0.62 | -11.98 | -29.92 | -10.32 |
| Three zones | HX-13 | 0.73 | -11.98 | -27.95 | -9.10 |
Table 8 shows the stable carbon isotope fractionation value Δ a for assimilating carbon dioxide, the stable carbon isotope fractionation value Δ b for assimilating bicarbonate, and the bicarbonate utilization fraction f for three regionalized aromatic leaves in August, general, Jiangyang, Guiyang cityb
The implementation effect of the invention is as follows:
as can be seen from the results of tables 2, 4, 6 and 8, the bicarbonate utilization shares are different in different regions of different plants, in different regions of the same plant, and even at different times of the same plant in the same region. The results in tables 2, 4, 6 and 8 are summarized in Table 9. As can be seen from tables 2, 4, 6, 8 and 9, the stable carbon isotope fractionation values of the carbon dioxide assimilated by different plants are significantly different, but the stable carbon isotope fractionation values of the carbon dioxide assimilated by the same plant in different months are slightly different, and the stable carbon isotope fractionation values of the carbon dioxide assimilated by all the materials are close to 20 ‰; the stable carbon isotope fractionation values of assimilating bicarbonate are different in different regions of different plants, different regions of the same plant, and even different time of the same plant in the same region, which shows that the utilization of plant bicarbonate has high variability, and the karst suitable plants can just utilize flexible and variable bicarbonate to adapt to the environment. Meanwhile, as can be seen from tables 2, 4, 6, 8 and 9, the utilization of camptotheca acuminata bicarbonate in three regions of majorany of Guiyang city is from 4.6% to 21.7%, the average value is 13.6%, the utilization of champignon bicarbonate is from 4.2% to 25.9%, and the average value is 15.3%. The utilization ratio of the camptotheca acuminata bicarbonate in three regions of May, Shandong, Guiyang city is from 6.0% to 33.8%, the average value is 18.8%, the utilization ratio of the champignon bicarbonate is from 3.0% to 30.5%, and the average value is 13.7%. These results for camptotheca acuminata were similar to those obtained by the two-way isotope-labelled culture technique in our laboratory. Therefore, the plants show the characteristic of adapting to diversified environments in the aspect of inorganic carbon utilization, namely the plants change the utilization of the inorganic carbon according to the environments so as to adapt to the environments.
Table 9 shows the stable carbon isotope fractionation value Δ a of assimilating carbon dioxide, the stable carbon isotope fractionation value Δ b of assimilating bicarbonate, and the utilization fraction f of bicarbonate in three regions of general radix et rhizoma Rhei, October, and folium Tulipae Bischatae in Guiyang cityb
Claims (5)
1. A method for obtaining the utilization portion of plant bicarbonate in a field habitat is characterized by comprising the following steps:
step one, selecting a second unfolded leaf blade on a new branch of a plant growing in a investigated environment, and respectively measuring 9 a.m. by using a portable photosynthetic apparatus in a clear weather: 00-10: a photosynthetic parameter of 00;
acquiring data of extracellular atmospheric carbon dioxide concentration Ca and intercellular carbon dioxide concentration Ci from the portable photosynthetic apparatus, and calculating the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaves to the extracellular atmospheric carbon dioxide concentration Ca;
selecting the leaves of the first unfolded leaf on the new branch of the plant growing in the investigated environment, and measuring the stable carbon isotope composition delta of the plant leaves by using a conventional method13The value of C, is noted as deltaT；
Step four, acquiring the stable carbon isotope composition delta of the carbon dioxide in the atmosphere of the investigated environment according to a conventional method13Average value of C, denoted as δa；
Step five, measuring the stable carbon isotope composition delta of the rhizosphere soil bicarbonate of the plants growing in the investigated environment according to a conventional method13The value of C, is noted as deltab；
Calculating a stable carbon isotope fractionation value delta a of assimilating carbon dioxide of the plant leaf growing in the investigated environment according to the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaf to the extracellular atmospheric carbon dioxide concentration Ca;
seventhly, calculating a stable carbon isotope fractionation value delta b of the plant leaf assimilating bicarbonate growing in the investigated environment according to the stable carbon isotope fractionation value delta a of the plant leaf assimilating carbon dioxide growing in the investigated environment;
step eight, according to the stable carbon isotope composition delta of the plant leaves growing under the investigated environment13Value delta of CTCarbon dioxide stable carbon isotope composition delta of the atmosphere to be investigated13Average value delta of CaStable carbon isotope composition delta of rhizosphere soil bicarbonate of plants grown in the investigated environment13Value delta of Cb(ii) a The stable carbon isotope fraction value Delta a of the plant leaf assimilating carbon dioxide grown in the examined environment and the stable carbon isotope fraction value Delta b of the plant leaf assimilating bicarbonate grown in the examined environment were calculated to calculate the plant bicarbonate utilization fraction fb(ii) a The utilization portion f of the plant bicarbonatebThe calculation method comprises the following steps:
2. the method for obtaining the utilization portion of the plant bicarbonate in the field habitat as claimed in claim 1, wherein the method comprises the following steps: in the first step, the leaves of the second unfolded leaves on the new branch of the plant are taken as the first completely unfolded leaves and the like according to the principle from top to bottom by taking the leaves which are just completely developed and completely unfolded on the new branch as the first completely unfolded leaves; the second fully unfolded leaf and the third fully unfolded leaf.
3. The method for obtaining the utilization portion of the plant bicarbonate in the field habitat as claimed in claim 1 is characterized in that: the ratio f of the intercellular carbon dioxide concentration Ci of the plant leaf to the extracellular atmospheric carbon dioxide concentration Ca in the step two is calculated by the following method:
4. the method for obtaining the utilization portion of the plant bicarbonate in the field habitat as claimed in claim 1, wherein the method comprises the following steps: the method for calculating the stable carbon isotope fractionation value Δ a for assimilating carbon dioxide by plant leaves growing in the examined environment, which is described in the sixth step, includes: delta a is 4.4 thousandths + (D-4.4 thousandths) f, wherein D is the fractionation of inorganic carbon during Rubsico carboxylation, and the range is 27 thousandths to 29 thousandths according to different values of plants.
5. The method for obtaining the utilization portion of the plant bicarbonate in the field habitat as claimed in claim 1, wherein the method comprises the following steps: in the seventh step, the method for calculating the stable carbon isotope fractionation value of assimilating bicarbonate by a plant leaf grown in the examined environment includes: delta b is delta a-4.4-9.9 per mill.
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