CN110628622A - Remove portable gas generator cell culture device - Google Patents
Remove portable gas generator cell culture device Download PDFInfo
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- CN110628622A CN110628622A CN201911100380.5A CN201911100380A CN110628622A CN 110628622 A CN110628622 A CN 110628622A CN 201911100380 A CN201911100380 A CN 201911100380A CN 110628622 A CN110628622 A CN 110628622A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 66
- 238000006243 chemical reaction Methods 0.000 claims description 63
- 238000007789 sealing Methods 0.000 claims description 25
- 230000007704 transition Effects 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 230000003139 buffering effect Effects 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims 2
- 239000007788 liquid Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 2
- 238000013142 basic testing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/54—Constructional details, e.g. recesses, hinges hand portable
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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Abstract
A mobile portable gas generation cell culture device belongs to the field of cell culture devices. At present, no specific gas generation cell culture device exists in the market. The utility model provides a remove portable gas generation cell culture device, between last top cap (1) at top and lower base (7) of bottom, the left side sets gradually from top to bottom goes up top cap (1), cell culture box (2), middle excessive connection structure (3), gas generation reation kettle (4), and the right side sets up gas flowmeter (5), and cell culture box (2) and gas generation reation kettle (4) all are connected with gas flowmeter (5) through a pipe (6). The invention can provide a completely sealed cell culture environment.
Description
Technical Field
The invention relates to a mobile portable gas generation cell culture device.
Background
In the fields of biology and medicine, cell culture is used as a basic experimental technology for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to ensure that the cells survive, grow and reproduce and maintain main structures and functions. And then the signal transduction of the cell, the anabolism of the cell, the growth and proliferation of the cell, and the like are studied. The invention relates to a culture device of cells under specific gas, which is used for testing basic testing parameters of biological experiments such as growth, reproduction, metabolism and the like of the cells under the specific gas. There is not special specific gas generation cell culture device in the existing market, and its technical key lies in that a special film structure can guarantee that gas stably passes through, and guarantees the leakproofness of whole device, guarantees that quantitative gas passes through, can block culture medium liquid simultaneously again and leak to realize the demand that the cell cultivates under specific gas condition.
Disclosure of Invention
The invention aims to solve the problem that no special targeted gas generating cell culture device exists in the market at present, and provides a mobile portable gas generating cell culture device.
A mobile portable gas generation cell culture device comprises an upper top cover, a cell culture box, a middle transition connection structure, a gas generation reaction kettle, a gas flowmeter, a guide pipe and a lower base, wherein the upper top cover at the top and the lower base at the bottom are arranged between the upper top cover at the top and the lower base at the bottom, the upper top cover, the cell culture box, the middle transition connection structure and the gas generation reaction kettle are sequentially arranged on the left side from top to bottom, the gas flowmeter is arranged on the right side, and the cell culture box and the gas generation reaction kettle are connected with the gas flowmeter through the guide pipe;
wherein the content of the first and second substances,
the gas reaction kettle comprises a reaction kettle upper cover, a reaction kettle lower cover, a reaction kettle, a gas outlet, a sealing washer and an injection port, wherein the reaction kettle upper cover is arranged above the reaction kettle lower cover, one side wall of the reaction kettle upper cover is communicated with the injection port, the other side wall of the reaction kettle upper cover is communicated with the gas outlet, the reaction kettle upper cover and the reaction kettle lower cover are connected in a detachable installation mode, and the reaction kettle is arranged in the reaction kettle lower cover and is sealed through the sealing washer; the gas outlet is connected with the conduit through a conduit connector flowmeter by processing threads at the connection part of the gas outlet and the conduit;
the cell culture box comprises a culture box upper cover, a culture box shell, an air buffer box, a culture dish, a sealing gasket and an air inlet; the upper cover of the culture box, the shell of the culture box and the gas buffer box are arranged from top to bottom in sequence, and the middle transition connection structure is arranged between the cell culture box and the gas generation reaction kettle; the culture dish is arranged in the shell, the culture dish, the shell and the gas buffer box are fixed, and the shell and the gas buffer box are connected in a detachable installation mode; the side wall of the gas buffering box is provided with a gas inlet, and a thread matched with the gas inlet and the guide pipe is processed at the joint between the gas inlet and the guide pipe, so that the connection is convenient; the bottom of the cell culture dish is provided with a group of conical through holes, and the aperture of the conical through holes is sequentially enlarged from inside to outside; a silica gel sealing gasket is arranged between the culture dish and the gas buffer box, and the culture dish extrudes the sealing gasket, so that the integral sealing performance is realized;
the upper top cover is provided with a handle, so that the whole portable movement is convenient, and the gas generation reaction kettle, the cell culture box and the upper top cover are provided with positioning holes which are matched with the lower bottom plate fixing rod for fixing and supporting.
The invention has the beneficial effects that:
the invention is a completely closed gas reaction generating structure, which is transmitted into a cell culture dish from gas generation to completely ensure sealing so as to ensure quantitative gas to pass through, thereby ensuring the authenticity and accuracy of experimental test.
The bottom surface of the cell culture dish is uniformly provided with a group of multi-conical through hole structures with the inner diameters gradually expanding from inside to outside, so that the one-way passing of gas can be realized, and the outflow of culture solution can be blocked, thereby realizing the cell culture under the specific gas.
The fastener of the gas reaction kettle and the clamping fastener of the cell culture box can play a role in pressurizing and sealing and can also provide a carrying function.
Drawings
FIG. 1 is a schematic external view of a mobile portable gas-generating cell culture apparatus;
FIG. 2 is a schematic view of the structure of a gas generation reactor section;
FIG. 3 is a schematic view of the exploded structure of the gas generation reactor section;
FIG. 4 is a schematic view of the structure of a cell culture cassette part;
FIG. 5 is a schematic exploded view of a cell culture cassette part;
FIG. 6 is a schematic view of an intermediate transition support portion;
FIG. 7 is a schematic view of the structure of the lower base portion;
fig. 8 is a schematic structural view of the upper top cover.
Detailed Description
The first embodiment is as follows:
a mobile portable gas-generating cell culture device of the present embodiment, as shown in fig. 1-8, comprises an upper top cover 1, a cell culture box 2, a middle transition connecting structure 3, a gas-generating reaction kettle 4, a gas flow meter 5, a conduit 6, a lower base 7, wherein the upper top cover 1 at the top and the lower base 7 at the bottom are arranged between the upper top cover 1 at the top and the lower base 7 at the bottom, the upper top cover 1, the cell culture box 2, the middle transition connecting structure 3, the gas-generating reaction kettle 4 are sequentially arranged on the left side from top to bottom, the gas flow meter 5 is arranged on the right side, and the cell culture box 2 and the gas-generating reaction kettle 4 are both connected with the gas flow meter 5;
the left, right, top and bottom of the present invention are described with respect to what is shown diagrammatically in the drawings.
Wherein the content of the first and second substances,
the gas reaction kettle 4 comprises a reaction kettle upper cover 4a, a reaction kettle lower cover 4b, a reaction kettle 4d, an air outlet 4e, a sealing washer 4f and an injection port 4g, wherein the reaction kettle upper cover 4a is arranged above the reaction kettle lower cover 4b, one side wall of the reaction kettle upper cover 4a is communicated with the injection port 4g, the opposite side wall is communicated with the air outlet 4e, the reaction kettle upper cover 4a is connected with the reaction kettle lower cover 4b in a detachable installation mode, and the reaction kettle 4d is arranged in the reaction kettle lower cover 4b and is sealed through the sealing washer 4 f; the gas outlet 4e is connected with the flowmeter 5 through a conduit 6, the connection part of the gas outlet 4e and the conduit 6 is processed with threads for convenient connection, the gas reaction kettle 4 is convenient to be connected with the conduit 6 and the gas flowmeter 5,
the cell culture box 2 comprises a culture box upper cover 2a, a culture box shell 2b, a gas buffer box 2c, a culture dish 2e, a sealing gasket 2f and an air inlet 2 g; the upper cover 2a of the culture box, the shell 2b of the culture box and the gas buffer box 2c are arranged from top to bottom in sequence, and the middle transition connecting structure 3 between the cell culture box 2 and the gas generation reaction kettle 4 plays a role in fixing and supporting; the culture dish 2e is arranged in the shell 2b, the culture dish 2e, the shell 2b and the gas buffer box 2c are fixed, and the shell 2b and the gas buffer box 2c are connected in a detachable installation mode; the side wall of the gas buffering box 2c is provided with a gas inlet 2g, and a thread matched with the gas inlet 2g is machined at the joint between the gas inlet 2g and the guide pipe 6, so that the connection is convenient; the bottom of the cell culture dish 2e is provided with a group of conical through holes, the aperture of the conical through holes is sequentially enlarged from inside to outside to form a multi-conical hole structure with a special structure, and the function of ventilation and liquid resistance can be realized; a silica gel sealing gasket 2f is arranged between the culture dish 2e and the gas buffer box 2c, and the culture dish 2e extrudes the sealing gasket 2f, so that the integral sealing performance is realized;
the upper top cover 1 is provided with a handle 1a which is convenient for integral portable movement, and the gas generation reaction kettle 4, the cell culture box and the upper top cover 1 are provided with positioning holes which are matched with a lower bottom plate fixing rod 7a for fixing and supporting.
The second embodiment is as follows:
different from the specific embodiment, the mobile portable gas generation cell culture device of the embodiment is as shown in fig. 1 to 8, wherein the lower bottom plate 7 comprises four pillars 7a and a bottom plate 7b, the upper surface of the left side of the bottom plate 7b is vertically provided with the four pillars 7a, the cell culture box 2, the middle transition connection structure 3 and the gas generation reaction kettle 4 are respectively provided with a plurality of through holes corresponding to the positions of the four pillars 7a, and the four pillars 7a are used for sequentially penetrating through the through holes from top to bottom so as to fix the culture box 2, the middle transition connection structure 3 and the gas generation reaction kettle 4 and support the three through the four pillars 7 a.
The third concrete implementation mode:
different from the specific embodiment, the mobile portable gas generation cell culture device of the present embodiment, as shown in fig. 1 to 8, the gas reaction kettle 4 further includes a fastener 4c, the fastener 4c includes a first handle 4a1 and a first fixing buckle block 4a2, two ends of the first handle 4a1 are hinged on an outer side wall of the reaction kettle lower cover 4b, the first fixing buckle block 4a2 is disposed on an outer side wall of the reaction kettle upper cover 4a, the first handle 4a1 is in a shape of a few frames, an upper surface of the first fixing buckle block 4a2 is provided with a groove, the outer side wall of the groove is an inclined surface, so that the first handle 4a1 slides into the groove from an outer side surface, the fastener 4c connects the reaction kettle upper cover 4a with the reaction kettle lower cover 4b, and the fastener 4c further plays roles of pressurization sealing and carrying the handle after disassembly and assembly.
The fourth concrete implementation mode:
the third difference from the specific embodiment is that, the mobile portable gas generation cell culture device of the embodiment, as shown in fig. 1-8, the cell culture box 2 further includes a fastening member 2d, the fastening member 2d includes a second handle 2a1 and a second fixing fastening block 2a2, two ends of the second handle 2a1 are hinged to an outer side wall of the gas buffer box 2c, the second fixing fastening block 2a2 is disposed on an outer side wall of the culture box housing 2b, the second handle 2a1 is in a shape of a few frames, an upper surface of the second fixing fastening block 2a2 is provided with a groove, the outer side wall of the groove is an inclined surface, so that the second handle 2a1 slides into the groove from the outer side surface, the fastening member 2d connects the culture box housing 2b with the gas buffer box 2c, and the fastening member 2d further plays roles of pressurization sealing and carrying the handle after disassembly and assembly.
The working principle is as follows:
with gas generation reation kettle 4, middle excessive connection structure 3 and cell culture box 2 loop through the locating hole and utilize dead lever 7a to fix left side on lower plate 7, also fix gas flowmeter 5 on lower plate 7 right side simultaneously, be connected reation kettle 4 and gas flowmeter 5 through pipe 6, be connected cell culture box 2 and gas flowmeter 5. Place cell culture liquid in the culture dish 2e of bell mouth when carrying out the experiment, put into glass ware reation kettle 4d with the chemical, through buckle 4c fastening upper cover and lower cover, compress tightly sealing silica gel gasket 4f between the upper and lower cover and realize holistic gas tightness. The reaction solution or reagent is injected through an injection hole 4g in the upper lid 4a of the reaction vessel. The injection hole 4g is locked, and the specific gas is continuously generated in the gas generation kettle 4 d. The gas generation reaction vessel 4 is connected to a gas flow meter 5 via a pipe 6, and the gas flows into the gas flow meter 5 through an outflow hole 4 e. The gas discharge amount is controlled by adjusting the flow rate of the gas flowmeter 5, so that the gas is quantitatively output. After passing through the gas flow meter 5, the gas generated by the chemical reaction flows into the cell culture box 2 with the same amount of gas output, the gas flow meter 5 is connected with the cell culture box 2 through a conduit 6, and the cell culture box 2 comprises a gas buffer box 2c for storing and buffering the inflow gas. When the gas buffer box 2c is completely filled with gas, the gas can enter the cell culture solution through the cell culture dish 2e with a special multi-cone hole structure. A special cell culture dish 2e with a multi-cone-shaped hole structure can be ventilated and blocked with liquid, a plurality of cone holes are uniformly distributed at the bottom of the culture dish, when gas is full in a gas buffer box, the gas pressure reaches the maximum, and the liquid can flow into the cell culture dish uniformly through the cone holes. The upper part aperture of the taper hole is only 0.1mm, and the liquid cannot flow out through the hole under the condition of low pressure due to the action of the surface tension of the liquid, so that the function of blocking liquid is realized.
After the experiment, the top cap 1 is covered by the accessible, locks four dead levers 7a with the nut, mentions handle 1a and realizes carrying and removing, simultaneously for convenient change of cultivateing box 2 and reation kettle 4, can detain buckle 2d and buckle 4c, realizes changing, utilizes buckle 2d and 4c to take away cell culture box 2 and gaseous 4 parts of taking place separately simultaneously, realizes portable change and removal.
Claims (4)
1. A mobile portable gas generation cell culture device is characterized in that: the device comprises an upper top cover (1), a cell culture box (2), a middle transition connecting structure (3), a gas generation reaction kettle (4), a gas flow meter (5), a guide pipe (6) and a lower base (7), wherein the upper top cover (1) at the top and the lower base (7) at the bottom are arranged between the upper top cover (1) at the top and the lower base (7) at the bottom, the upper top cover (1), the cell culture box (2), the middle transition connecting structure (3) and the gas generation reaction kettle (4) are sequentially arranged on the left side from top to bottom, the gas flow meter (5) is arranged on the right side, and the cell culture box (2) and the gas generation reaction kettle (4) are connected with the gas flow;
wherein the content of the first and second substances,
the gas reaction kettle (4) comprises a reaction kettle upper cover (4 a), a reaction kettle lower cover (4 b), a reaction kettle (4 d), a gas outlet (4 e), a sealing washer (4 f) and an injection port (4 g), wherein the reaction kettle upper cover (4 a) is arranged above the reaction kettle lower cover (4 b), one side wall of the reaction kettle upper cover (4 a) is communicated with the injection port (4 g), the other side wall is communicated with the gas outlet (4 e), the reaction kettle upper cover (4 a) is connected with the reaction kettle lower cover (4 b) in a detachable installation mode, and the reaction kettle (4 d) is arranged in the reaction kettle lower cover (4 b) and is sealed through the sealing washer (4 f); the gas outlet (4 e) is connected with the flowmeter (5) through the conduit (6), and the connection part of the gas outlet (4 e) and the conduit (6) is processed with threads for convenient connection;
the cell culture box (2) comprises a culture box upper cover (2 a), a culture box shell (2 b), an air buffer box (2 c), a culture dish (2 e), a sealing gasket (2 f) and an air inlet (2 g); the upper cover (2 a) of the culture box, the shell (2 b) of the culture box and the gas buffer box (2 c) are arranged from top to bottom in sequence, and the middle transition connecting structure (3) is arranged between the cell culture box (2) and the gas generation reaction kettle (4); the culture dish (2 e) is arranged in the shell (2 b), the culture dish (2 e), the shell (2 b) and the gas buffer box (2 c) are fixed, and the shell (2 b) and the gas buffer box (2 c) are connected in a detachable installation mode; the side wall of the gas buffering box (2 c) is provided with a gas inlet (2 g), and a thread matched with the gas inlet (2 g) and the guide pipe (6) is machined at the joint between the gas inlet (2 g) and the guide pipe for convenient connection; the bottom of the cell culture dish (2 e) is provided with a group of conical through holes, and the aperture of the conical through holes is enlarged from inside to outside in sequence; a silica gel sealing gasket (2 f) is arranged between the culture dish (2 e) and the gas buffer box (2 c), and the culture dish (2 e) extrudes the sealing gasket (2 f), so that the integral sealing performance is realized;
the upper top cover (1) is provided with a handle (1 a) which is convenient for integral portable movement, and the gas generation reaction kettle (4), the cell culture box and the upper top cover (1) are provided with positioning holes which are matched with a lower bottom plate fixing rod (7 a) for fixing and supporting.
2. A mobile portable gas-generating cell culture apparatus of claim 1, wherein: lower plate (7) include four spinal branch posts (7 a), bottom plate (7 b), four spinal branch posts (7 a) are installed to the vertical upper surface on bottom plate (7 b) left, cell culture box (2), middle excessive connection structure (3), it is individual with the corresponding through-hole in position of four spinal branch posts (7 a) to set up respectively all around of gaseous reation kettle (4) that takes place, four spinal branch posts (7 a) are used for passing the through-hole from top to bottom in proper order, with to cultivate box (2), middle excessive connection structure (3) and gaseous reation kettle (4) are fixed, and support the three through four spinal branch posts (7 a).
3. A mobile portable gas-generating cell culture apparatus according to claim 1 or 2, wherein: gaseous reation kettle (4) still include fastener (4 c), fastener (4 c) including first handle (4 a 1) and first fixed knot piece (4 a 2), the both ends of first handle (4 a 1) articulate on the lateral wall of reation kettle lower cover (4 b), first fixed knot piece (4 a 2) set up on the lateral wall of reation kettle upper cover (4 a), first handle (4 a 1) are several word frame types, first fixed knot piece (4 a 2) upper surface has the recess, the lateral wall of recess is the inclined plane, so that first handle (4 a 1) slides into the recess from the lateral surface in, thereby make fastener (4 c) be connected reation kettle upper cover 4a and reation kettle lower cover 4b, fastener 4c still plays the effect of the portable handle of pressurization sealing and dismouting back.
4. A mobile portable gas-generating cell culture apparatus of claim 3, wherein: cell culture box (2) still include buckle spare (2 d), buckle spare (2 d) include second handle (2 a 1) and the fixed knot piece of second (2 a 2), the both ends of second handle (2 a 1) articulate on the lateral wall of gas buffer box (2 c), the fixed knot piece of second (2 a 2) sets up on the lateral wall of culture box shell (2 b), second handle (2 a 1) are several word frame types, the fixed knot piece of second (2 a 2) upper surface has the recess, the lateral wall of recess is the inclined plane, so that second handle (2 a 1) slides into the recess from the lateral surface, thereby make buckle spare (2 d) be connected culture box shell (2 b) and gas buffer box (2 c), buckle spare (2 d) still play the effect of pressurization sealing and the portable handle after the dismouting.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911100380.5A CN110628622A (en) | 2019-11-12 | 2019-11-12 | Remove portable gas generator cell culture device |
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| CN201911100380.5A CN110628622A (en) | 2019-11-12 | 2019-11-12 | Remove portable gas generator cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528342A (en) * | 2021-07-16 | 2021-10-22 | 苏州奥芮济医疗科技有限公司 | Air-permeable embedded cell culture chamber and preparation method thereof |
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2019
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