CN107356708A - A kind of Portable field CO2Isotope marks device and application method - Google Patents
A kind of Portable field CO2Isotope marks device and application method Download PDFInfo
- Publication number
- CN107356708A CN107356708A CN201710477846.8A CN201710477846A CN107356708A CN 107356708 A CN107356708 A CN 107356708A CN 201710477846 A CN201710477846 A CN 201710477846A CN 107356708 A CN107356708 A CN 107356708A
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- China
- Prior art keywords
- portable field
- isotope marks
- shaped support
- support
- mark
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
Abstract
The present invention provides a kind of Portable field CO2Isotope marks device, labelling apparatus agent structure includes umbrella shaped support device and the sealing device being sleeved on outside umbrella shaped support device, umbrella shaped support device is Collapsible structure, including support bar, the slide fastener being placed on the support bar, the main umbrella skeleton being connected with the supporting bar top, all connected between main umbrella skeleton and slide fastener described in every by expansion bracket, the sealing device offers mark CO2Air inlet, the support bar bottom, which is extended, support, and the support is made up of steel pipe splicing, is a kind of simple effective, dismountable Portable field13CO2Isotope marks device.The present invention also provides a kind of Portable field CO2The application method of isotope marks device, closing development can be carried out to the canopy of whole mao bamboon13CO2Labelling experiment, simple in construction, easy to operate, mark success rate height.
Description
Technical field
The invention belongs to tagging field, and in particular to a kind of dress with Carbon isotope gas labeled plant
Put.
Background technology
13C is stable isotope, and its labelling technique is more ripe at home and abroad and is widely used in plant physiology life
Each field of scientific study such as state, Biogeochemistry, climate change.And the method for isotope marks mainly has three at present
Kind：Natural abundance method, continued labelling and pulse labeling.Natural abundance method is showed based on what plant and plant isotope point were presented
As；Continued labelling needs injection label for a long time, is generally suitable for evaluating plant input soil and underground carbon storehouse total amount is ground
Study carefully；Pulse labeling refers to once inject a certain amount of label, can be used to study point of the plant in particular growth period photosynthetic carbon
With situation, typically adopted this method in the distribution research of plant photosynthesis product.At present, the mark of stable carbon isotope is passed through
The physiological metabolism and Nutrient Cycling that tracer technique can reflect in plant, can be as the important indicator for assessing plant physiology function.
Tracer method refers to that be tracked material with tracer (labeled atom or labeled compound) research is in experiment
Motion, the method for transformation rule in system.It can be used as a kind of instrument, for scientific experiment, study the motion of material, conversion is gone through
Journey, disclose objective law.Biologically generally use tracer method studies material or element in vivo at present
Transfer regularity.
At present have to bough strip adoption mark mode carry out tagging device, these devices can quickly, nothing
Branch is marked for loss, but is directed to for whole tree, also without effective easy CO2Labelling apparatus.
It is existing13CO2Labelling apparatus is largely positioned in laboratory, and majority is used for potted plant herbaceous plant or trees seedling
Indoor labelling experiment, poor mobility, be not used to field trial.Oneself of labeled in situ experiment is carried out to trees in the wild at present
Device processed is only applicable to the mark of a part of branch of trunk and leaf etc., it is impossible to large-scale whole tree CO2Transhipment and nutrient
Conveying carries out systematic research, therefore the research for a wide range of whole tree has significant limitation.
The content of the invention
To solve above technical problem, the present invention provides a kind of simple effective, dismountable Portable field13CO2Same position
Plain labelling apparatus and application method, closing development can be carried out to the canopy of whole mao bamboon13CO2Labelling experiment, simple in construction, behaviour
Make convenient, mark success rate height.
To achieve the above object, the invention provides following scheme：
The present invention provides a kind of Portable field CO2Isotope marks device, agent structure include umbrella shaped support device and
The sealing device being sleeved on outside the umbrella shaped support device, the umbrella shaped support device are Collapsible structure, including support bar, are placed on
Slide fastener on the support bar, the main umbrella skeleton being connected with the supporting bar top, between the main umbrella skeleton and slide fastener described in every all
Connected by expansion bracket, the sealing device offers mark CO2Air inlet, the support bar bottom, which is extended, support,
The support is made up of steel pipe splicing.
Optionally, the umbrella shaped support equipment matter is stainless steel；
Optionally, it is provided with cushion at the umbrella shaped support device bottom margin；
Optionally, the cushion is sponge；
Optionally, the sealing device is made up of transparent sealing film, drawstring, nylon cable tie；
Optionally, CO2Isotope marks device is additionally provided with fan, and fixed position is located at the inside of the sealing device simultaneously
Close to lower end, the external battery of fan；
Optionally, CO2Isotope marks device is additionally provided with CO2Concentration detector, fixed position are located at the sealing device
Inside；
Optionally, the mark CO2Air inlet is quality of rubber materials；
Optionally, the mark CO2Air inlet end face is circular, a diameter of 3cm；
The present invention also provides a kind of Portable field CO2The application method of isotope marks device,
1. opening umbrella shaped support device, choose sealing device and be sleeved on umbrella shaped support device；
2. umbrella shaped support device edge and transparent sealing film are fixed, and cushion is set；
3. whole device is slowly lifted upwards up to the top more than mao bamboon, steel pipe is suitably adjusted according to the height of the leaf of bamboo
Quantity, support bar bottom and support are connected, and support bar and support are fixed on bamboo bar.
4. by fan and CO2Concentration detector is fixed on bamboo bar after opening, drawstring of then leaving behind, by transparent sealing film
The whole leaf of bamboo is covered, transparent sealing film is collected and is entangled in together on bamboo bar, and sealing is tightened with Nylon Bag；
5. from mark CO2Air inlet inputs a certain amount of mark CO2, experiment is marked.
The present invention achieves following technique effect relative to prior art：
1st, Portable field of the present invention13CO2Isotope marks device is provided with support, and support is stainless by several 2 meters long
Steel steel pipe splicing composition, while for propping up umbrella shaped support device, also act as and adapted to not by adjusting the quantity of splicing steel pipe
It is stronger with the effect of height mao bamboon, adaptability.
2nd, Portable field of the present invention13CO2The support meanss of isotope marks device are made up of skeleton in bulk, detachable,
It is adjustable, it is easy to fixed and installs, it is more convenient to fetch.
Portable field provided by the invention13CO2Isotope marks device, its simple structure effectively, detachably, can solve
Certainly existing field trial device can only carry out the plant organs such as part branch and blade and be marked and detect, and cause result of the test
With limitation and it is uncertain the problem of so that the Portable field13CO2Isotope marks device can enter to whole tree crown
Row sealing CO2Mark test, and result of the test is more accurate, simple in construction, easy to operate, mark success rate height.
The present invention is using the whole canopy of mao bamboon as tagged object, there is provided a kind of simple effective, dismountable portable13CO2
Labelling apparatus and application method, closing development can be carried out to the canopy of whole mao bamboon13CO2Labelling experiment, this is whole for studying
The carbon cycle important in inhibiting of individual Phyllostachys Pubescens Ecosystem, to carrying out other tree crown taper type trees13CO2Labelling experiment
There are important reference and reference value.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing needed to use is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is a kind of Portable field in inventive embodiments13CO2Isotope marks schematic device；
Description of reference numerals：1st, cushion；2nd, umbrella shaped support device；2-1, support bar；2-2, slide fastener；2-3 main chute bars；2-4, stretch
Contracting frame；3rd, sealing device；4th, CO is marked2Air inlet；5th, fixed part；6th, support；7th, fan；8、CO2Concentration detector.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
It is an object of the invention to provide a kind of Portable field13CO2Isotope marks device, the apparatus structure is simple, can
Dismounting, can solve the problem that existing field trial device can only carry out the plant organs such as part branch and blade and be marked and detect,
Cause result of the test have limitation and it is uncertain the problem of so that the Portable field13CO2Isotope marks device can
Sealing CO is carried out to whole tree crown2Mark test, and result of the test is more accurate, the present invention have it is simple in construction, easy to operate,
Mark the features such as success rate height.
In order to facilitate the understanding of the purposes, features and advantages of the present invention, it is below in conjunction with the accompanying drawings and specific real
Applying example, the present invention is further detailed explanation.
The present invention provides a kind of Portable field13CO2Isotope marks device, as shown in figure 1, labelling apparatus agent structure
Including umbrella shaped support device 2 and sealing device 3, the size of umbrella shaped support device 2 will guarantee to cover on all leaf of bamboves, wherein sealing
Device 3 is sleeved on the outside of umbrella shaped support device 2, and umbrella shaped support device 2 is arranged to Collapsible structure, including support bar 2-1, slide fastener 2-
2nd, main umbrella skeleton 2-3 and expansion bracket 2-4, slide fastener 2-2 are placed on support bar 2-1, and main umbrella skeleton 2-3 is connected with support bar 2-1 tops,
All connected between every main umbrella skeleton 2-3 and slide fastener 2-2 by expansion bracket 2-4, and then ensure that the stability cocoa of stretching structure
By property.
In order to facilitate progress13CO2Isotope marks, sealing device are provided with mark CO2Air inlet 4, and be directly opened in close
On seal apparatus 3, CO is marked2Air inlet 3 selects quality of rubber materials, inputs gas for convenience, marks CO2The end face of air inlet 3 is set
For circle, for the ease of the control to inputting gas flow, CO is marked2The diameter of air inlet 3 is arranged to 3cm.
Support bar 2-1 bottoms, which are extended, support 6, and support 6 is made up of the steel pipe splicing of varying number, different length,
The adjustability of umbrella shaped support device 2 and the height of sealing device 3 is ensure that, in order to adapt to the mark test of different height plant.
In order to prevent corrosion, and the stability of sealing device 3 is further ensured that, and then ensures the experiment of safety and stability
Environment, the material of support bar 2-1, main umbrella skeleton 2-3, expansion bracket 2-4, support 6 is disposed as having some strength and rigidity
Stainless steel.
Sealing device 3 is made up of transparent sealing film, drawstring, nylon cable tie, further, in the bottom sides of umbrella shaped support device 2
Cushion 1 is set at edge, and further elects cushion material as sponge, for preventing transparent sealing film by the sharp of support meanss
Poke part.
CO2Isotope marks device is additionally provided with fan 7, and fixed position is located at the inside of sealing device 3 and close lower end,
7 external battery of fan, fan size are 10cm × 20cm or so, weight 100g or so, by battery supplied electric power, agitation air,
Ensure the CO in sealing device2Uniform concentration distribution, prevent CO2Because density is big and under be sunken to sealing device bottom, be further ensured that
The accuracy of experiment.
CO2Isotope marks device is additionally provided with CO2Concentration detector 8, it can be fixed on bamboo bar, and positioned at close
The inside of seal apparatus 3, CO2Concentration detector selection is hand-held pump suction type, and size is 8cm × 15cm or so, heavy 500g or so, point
Resolution 1ppm, brand are unlimited.
The present invention also provides a kind of Portable field CO2The application method of isotope marks device, its step are as follows：
1. opening umbrella shaped support device 2, choose sealing device 3 and be sleeved on umbrella shaped support device 2；
2. the edge of umbrella shaped support device 2 and transparent sealing film are fixed, and cushion 1 is set；
3. whole device is slowly lifted upwards up to the top more than mao bamboon, steel pipe is suitably adjusted according to the height of the leaf of bamboo
Quantity, support bar 2-1 bottoms and support 6 are connected, and support bar 2-1 and support 6 are fixed on bamboo bar.
4. by fan and CO2Concentration detector is fixed on bamboo bar after opening, drawstring of then leaving behind, by transparent sealing film
The whole leaf of bamboo is covered, transparent sealing film is collected and is entangled in together on bamboo bar, and sealing is tightened with Nylon Bag；
5. from mark CO2Air inlet 4 inputs a certain amount of mark CO2, experiment is marked.
As can be seen here, Portable field of the invention13CO2Isotope marks device, its simple structure is effective, detachable,
It can solve the problem that existing field trial device can only carry out the plant organs such as part branch and blade and be marked and detect, cause to try
Test result have limitation and it is uncertain the problem of so that the Portable field13CO2Isotope marks device can be to whole
Tree crown carries out sealing CO2Mark test, and result of the test is more accurate, simple in construction, easy to operate, mark success rate height.
It should be noted that the Portable field of the present invention13CO2Support bar, main umbrella skeleton in isotope marks device, stretch
Contracting frame, the material of support are not limited to a kind of stainless steel this material, as long as there is some strength and rigidity material, Neng Goubao
Demonstrate,prove the stable and firm of whole experimental provision.CO2The end face of air inlet is not limited to circle, can also be equilateral triangle,
The other shapes such as square, in addition, mark CO2The diameter of air inlet, which sets also to differ, is set to 3cm, can be according to actual conditions voluntarily
Setting.The material of cushion is also not limited to sponge, as long as the softness that transparent sealing film is poked by sharp parts can be prevented
The thing of material.The support that support bar bottom is connected is spliced by steel pipe, diameter, length and the quantity of steel pipe
It can be adjusted according to actual tests.
Apply specific case in this specification to be set forth the principle and embodiment of the present invention, above example
Explanation be only intended to help understand the present invention method and its core concept；Meanwhile for those of ordinary skill in the art,
According to the thought of the present invention, in specific embodiments and applications there will be changes.In summary, in this specification
Appearance should not be construed as limiting the invention.
Claims (10)
- A kind of 1. Portable field CO2Isotope marks device, it is characterised in that：Labelling apparatus agent structure includes umbrella shaped support Device (2) and the sealing device (3) for being sleeved on umbrella shaped support device (2) outside, the umbrella shaped support device (2) is scalable knot Structure, including support bar (2-1), be placed on the support bar (2-1) slide fastener (2-2), be connected with the support bar (2-1) top Main umbrella skeleton (2-3), all connected by expansion bracket (2-4) between the main umbrella skeleton (2-3) and slide fastener (2-2) described in every, it is described Sealing device (3) offers mark CO2Air inlet (4), support bar (2-1) bottom, which is extended, support (6), the branch Frame (6) is made up of steel pipe splicing.
- 2. Portable field CO according to claim 12Isotope marks device, it is characterised in that：The umbrella shaped support device (2) material is stainless steel.
- 3. Portable field CO according to claim 12Isotope marks device, it is characterised in that：The umbrella shaped support device (2) cushion is provided with bottom margin.
- 4. Portable field CO according to claim 32Isotope marks device, it is characterised in that：The cushion is sea It is continuous.
- 5. Portable field CO according to claim 12Isotope marks device, it is characterised in that：The sealing device (3) it is made up of transparent sealing film, drawstring, nylon cable tie.
- 6. Portable field CO according to claim 12Isotope marks device, it is characterised in that：CO2Isotope marks Device is additionally provided with fan (7), and fixed position is located at the inside of the sealing device (3) and close to lower end, and the fan (7) is outside Connect battery.
- 7. Portable field CO according to claim 12Isotope marks device, it is characterised in that：CO2Isotope marks Device is additionally provided with CO2Concentration detector (8), fixed position are located at the inside of the sealing device (3).
- 8. Portable field CO according to claim 12Isotope marks device, it is characterised in that：The mark CO2Enter Gas port (4) is quality of rubber materials.
- 9. Portable field CO according to claim 82Isotope marks device, it is characterised in that：The mark CO2Enter Gas port (4) end face is circular, a diameter of 3cm.
- A kind of 10. Portable field CO2The application method of isotope marks device, it is characterised in that：1. opening umbrella shaped support device (2), choose sealing device (3) and be sleeved on umbrella shaped support device (2)；2. umbrella shaped support device (2) edge is fixed with transparent sealing film, and cushion is set；3. whole device is slowly lifted upwards up to the top more than mao bamboon, steel pipe number is suitably adjusted according to the height of the leaf of bamboo Amount, support bar (2-1) bottom and support (6) is connected, and support bar (2-1) and support (6) are fixed on bamboo bar.4. by fan (7) and CO2Concentration detector (8) is fixed on bamboo bar after opening, and drawstring of then leaving behind is thin by transparent sealing Film covers the whole leaf of bamboo, and transparent sealing film is collected and is entangled in together on bamboo bar, and tightens sealing with Nylon Bag；5. from mark CO2Air inlet (4) inputs a certain amount of mark CO2, experiment is marked.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710477846.8A CN107356708A (en) | 2017-06-22 | 2017-06-22 | A kind of Portable field CO2Isotope marks device and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710477846.8A CN107356708A (en) | 2017-06-22 | 2017-06-22 | A kind of Portable field CO2Isotope marks device and application method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107356708A true CN107356708A (en) | 2017-11-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710477846.8A Pending CN107356708A (en) | 2017-06-22 | 2017-06-22 | A kind of Portable field CO2Isotope marks device and application method |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113943A (en) * | 2006-07-28 | 2008-01-30 | 中国科学院沈阳应用生态研究所 | Trees volatile organic chemistry material collecting device |
| CN201894089U (en) * | 2010-11-16 | 2011-07-13 | 香港城市大学深圳研究院 | Device for adopting stable carbon isotope gas for marking plants |
| CN102589923A (en) * | 2012-02-10 | 2012-07-18 | 浙江大学 | Plant 13CO2 mark cultivation-root exudate collection integration device |
| CN103598025A (en) * | 2013-10-28 | 2014-02-26 | 中国科学院东北地理与农业生态研究所 | Field in-situ continuous low-abundance 13CO2 isotope labeling device and method |
| CN204047298U (en) * | 2014-09-19 | 2014-12-31 | 石河子大学 | A kind of feeding device utilizing tagging plant |
| CN204903503U (en) * | 2015-08-21 | 2015-12-23 | 中国科学院南京土壤研究所 | Carbon isotope mark device |
| CN106248874A (en) * | 2016-07-22 | 2016-12-21 | 北京林业大学 | Isotope13c(13cO2) integration unit of labelling bough bar and labeling method |
-
2017
- 2017-06-22 CN CN201710477846.8A patent/CN107356708A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113943A (en) * | 2006-07-28 | 2008-01-30 | 中国科学院沈阳应用生态研究所 | Trees volatile organic chemistry material collecting device |
| CN201894089U (en) * | 2010-11-16 | 2011-07-13 | 香港城市大学深圳研究院 | Device for adopting stable carbon isotope gas for marking plants |
| CN102589923A (en) * | 2012-02-10 | 2012-07-18 | 浙江大学 | Plant 13CO2 mark cultivation-root exudate collection integration device |
| CN103598025A (en) * | 2013-10-28 | 2014-02-26 | 中国科学院东北地理与农业生态研究所 | Field in-situ continuous low-abundance 13CO2 isotope labeling device and method |
| CN204047298U (en) * | 2014-09-19 | 2014-12-31 | 石河子大学 | A kind of feeding device utilizing tagging plant |
| CN204903503U (en) * | 2015-08-21 | 2015-12-23 | 中国科学院南京土壤研究所 | Carbon isotope mark device |
| CN106248874A (en) * | 2016-07-22 | 2016-12-21 | 北京林业大学 | Isotope13c(13cO2) integration unit of labelling bough bar and labeling method |
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Application publication date: 20171117 |