CN107969243A - A kind of carbon isotope labelling device of clone plant independence plant division - Google Patents
A kind of carbon isotope labelling device of clone plant independence plant division Download PDFInfo
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- CN107969243A CN107969243A CN201610932564.8A CN201610932564A CN107969243A CN 107969243 A CN107969243 A CN 107969243A CN 201610932564 A CN201610932564 A CN 201610932564A CN 107969243 A CN107969243 A CN 107969243A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/02—Treatment of plants with carbon dioxide
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D21/00—Measuring or testing not otherwise provided for
- G01D21/02—Measuring two or more variables by means not covered by a single other subclass
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The present invention relates to a kind of carbon isotope labelling device of clone plant independence plant division, it is characterised in that：It is made of mark case (1) and temperature control water tank (2) two large divisions, is connected between the two by outlet pipe (11) and water inlet pipe (10)；Mark the side wall of case (1) that Hygrothermograph (4), CO2 concentration and air pressure tester (5), illumination photometer (6) are set, bottom sets gas circulator (7) and air inlet (8), case (1) is marked using double-deck water-filling side wall (13), its top sets pressure reducing valve (9), whole mark case (1) is placed on buttress (12), the interior set temperature measure of temperature control water tank (2) and warning device (19).The present invention realizes that the carbon labeling experiment of clone plant independence plant division simulates outdoor conditions completely, and experimental result is accurately and reliably；The present apparatus solves the soil carbon sedimentation in gas isotope labeling process, the excessive key technical problem of case body temperature；Present apparatus energy consumption is small, and cost is low.
Description
Technical field
The present invention relates to the same position of carbon in plant isotope labelling techniques field, more particularly to a kind of clone plant independence plant division
Plain labelling apparatus.
Background technology
Isotope is that have one of two or more atoms of same chemical element of same atoms ordinal number, in period of element
Occupy same position on table, chemical behavior is almost identical, but atomic weight or mass number are different, can be divided into stable isotope and put
Injectivity isotope.As a kind of dynamical tracer, carbon isotope (11CO2、13C and14C) in plant ecosystem carbon cycle
It is used widely in research.
The component of clone plant can flexibly adjust different component acquisition to realize that the division of labor in society provides necessary condition
The relative ability of different resource, make single plant division in order to obtain abundant resource and the Foote that becomes.Meanwhile it is also between plant division
Interdepend, work in coordination, acted on by Clonal integration, transmit exchanging material between plant division frequently by lateral connection thing.Gram
The division of labor in society of grand plant is theoretical, and a piece of frontier has been opened up for phytological research, be modern plants research hot spot with
Breach, has high ecology and evolutionary significance.Carbon isotope labelling method is a kind of ideal method for studying this physiology course,
But not only the prices of raw materials are more expensive due to carbon isotope labelling, it is often more important that CO2Easily diffusion, particularly14C-CO2Belong to radioactivity
Gas, therefore when carrying out plants carbon isotope labelling there are certain technical difficulty, this is also clone plant division of labor in society reason
By the bottleneck of research.It is related to that clone plant carbon isotope labelling mainly authorizes or disclosed patent includes at present：With stability carbon
The device (201020608969.4) of isotope gas labeled plant, a kind of field continuous low abundance in situ13CO2 isotope marks
A kind of carbon isotope labelling device (201420736248.X) of device and method (201310513820.6), a kind of carbon isotope mark
Remember device (201520633964.X).Although these researchers attempt to carry out some improvement on the device of Jenkinson,
It is that many problems are still had in terms of clone plant research, especially realizes the independence for the single plant division that rhizome transversely connected to
Mark, solves the soil carbon sedimentation in gas isotope labeling process, these excessive key issues of case body temperature are at present not yet
Solve.And the present invention is directed to disadvantage mentioned above, the new technology developed realizes whole-process automatic control, effective to promote clone to plant
The development of thing experiment.
The content of the invention
The object of the present invention is to provide a kind of carbon isotope labelling device of clone plant independence plant division, single plant division is realized
Independent marking, solve gas isotope labeling process in soil carbon sedimentation, the excessive key issue of case body temperature.
The technical proposal of the invention is realized in this way, a kind of carbon isotope labelling device of clone plant independence plant division,
It is characterized in that：It is made of mark case and temperature control water tank two large divisions, marks top between case and temperature control water tank to be connected by outlet pipe
Connect, lower part is connected by water inlet pipe；Mark the side wall of case that Hygrothermograph, CO2 concentration and air pressure tester, illumination photometer, bottom are set
Gas circulator, axis mouth and air inlet are set, and for mark case using double-deck water-filling side wall, its top sets pressure reducing valve, double-deck
The bottom of water-filling side wall sets water outlet, whole to mark case to be placed on buttress；The stem of labeled plant is arranged at axis mouth position
Put, setting axis set in axis mouth, set temperature measure and warning device in temperature control water tank.
The mark case side wall and top use transparent film or transparent glass preparation；Temperature control water tank and mark case are double
Be full of distilled water in layer water-filling side wall, water is 10~15 times of water in bilayer water-filling side wall in temperature control water tank；Water inlet pipe and
Outlet pipe and the angle of horizontal direction are more than 30 degree, and water inlet pipe and diameter of outflow tube are 20~50mm, mark case and temperature control water tank
Vertical clearance from for 0.5~3.0m.
The temperature measuring and warning device is arranged at temperature control water tank inner wall, its position height is temperature control water tank height
0.35~0.55 times；The top of water tank sets water inlet, its diameter or the length of side are 0.05~0.10m；Double-deck water-filling side wall bottom
Water outlet diameter or the length of side be 0.04~0.08m.
The mark bottom portion is with top to combine formation, and groove is left in bottom, and when top is combined with bottom, bilayer fills
The bottom of water side wall is put into groove, while it is two boards along split line split to use the bottom of fluid sealant sealing mark case
Form, mosaic is sealed using fluid sealant, while both ends are fixed using fixing piece.
The bottom plate air inlet of the mark case is self-seal device, the gas circulator that mark bottom portion is set, both sides
It is arranged symmetrically, opens daily 4~8 hours, CO2 concentration and air pressure tester should be in gas circulation pump operation 20min when measuring
Carry out later.
The temperature measuring and warning device are when temperature exceedes 5 DEG C of outside air temperature, automatic alarm, and when alarm needs
Cold water is replaced, the cold water temperature of replacement is 5~15 DEG C, the distillation water cooling system that cold water is handled for tap water by distillation
It is standby.
The pressure reducing valve mark case in pressure be more than outside atmospheric pressure 5% when, pressure reducing valve open, in pressure reducing valve
Before unlatching, gas circulator answer out of service 5 it is small when more than.
The Hygrothermograph measure should be measured when every day interval 2~4 is small, when humidity is less than outside humidity, be passed through
Air inlet adds water to be humidified；Illumination photometer is arranged at 0.5~0.8 times of bottom of mark spacing height and position, it measures early daily, neutralization evening
Respectively no less than once.
The good effect of apparatus of the present invention is that the carbon labeling experiment for realizing clone plant independence plant division completely may be used
Tested under conditions of control, test simulation outdoor conditions completely, experimental result is accurately and reliably；The present apparatus solves gas isotope
Soil carbon sedimentation in labeling process, the excessive key issue of case body temperature；Apparatus of the present invention simple structure, energy consumption is small, can
Strong operability, cost are low.
Brief description of the drawings
Fig. 1 is the profile of apparatus of the present invention
Fig. 2 is the bottom plan view of apparatus of the present invention
1. mark case, 2. temperature control water tanks, 3. axis sets, 4. Hygrothermographs, 5.CO2 concentration and air pressure tester in figure,
6. illumination photometer, 7. gas circulators, 8. air inlets, 9. pressure reducing valves, 10. water inlet pipes, 11. outlet pipes, 12. buttresses, 13. bilayers fill
Water side wall, 14. axis mouths, 15. fixing pieces, 16. spellings and line, 17. water outlets, 18. water inlets, 19. temperature measurings and alarm
Device, 20. labeled plants.
Embodiment
Apparatus of the present invention can realize the carbon labeling experiment of clone plant independence plant division, at the same with prior art phase
Than improving the accurate reliability of experimental result.
1 and 2 pair of the technology of the present invention content is described further below in conjunction with the accompanying drawings：
It is prepared by invention device
As depicted in figs. 1 and 2, the mark case 1 and temperature control water tank 2 of the invention device are prepared, marks case 1 and temperature control water tank 2
Between top connected using outlet pipe 11, lower part is connected using water inlet pipe 10, water inlet pipe 10 and outlet pipe 11 and horizontal direction
Angle is more than 30 degree, 11 a diameter of 20~50mm of water inlet pipe 10 and outlet pipe, mark the vertical clearance of case 1 and temperature control water tank 2 from
For 0.5~3.0m；Mark 1 side wall of case and top use transparent film or transparent glass preparation, are set in the side wall of mark case 1
Hygrothermograph 4, CO2 concentration and air pressure tester 5, illumination photometer 6, bottom set gas circulator 7, axis mouth 14 and air inlet
8, for mark case 1 using double-deck water-filling side wall 13, its top sets pressure reducing valve 9, and the bottom of double-deck water-filling side wall 13 sets water outlet
17, it is whole to mark case 1 to be placed on buttress 12；Axis is set to cover 3 in axis mouth 14, set temperature measures in temperature control water tank 2
And warning device 19；Distilled water is full of in temperature control water tank 2 and the double-deck water-filling side wall 13 of mark case 1, water is double in temperature control water tank 2
10~15 times of water in layer water-filling side wall 13.
With top to combine formation, groove is left in bottom for mark 1 bottom of case, when top is combined with bottom, double-deck water-filling side
The bottom of wall 13 is put into groove, while is sealed using fluid sealant；Spelled for two boards along split line 16 bottom of mark case 1
Conjunction forms, and mosaic is sealed using fluid sealant, while is fixed using fixing piece 15；The bottom plate air inlet 8 for marking case 1 is self sealss
Device, the gas circulator 7 that 1 bottom of mark case is set, both sides are arranged symmetrically；Temperature measuring and warning device 19 are arranged at temperature control
2 inner wall of water tank, its position height are 0.35~0.55 times of 2 height of temperature control water tank；The top of water tank sets water inlet 18, its is straight
Footpath or the length of side are 0.05~0.10m；17 diameter of water outlet or the length of side of double-deck 13 bottom of water-filling side wall are 0.04~0.08m；According to
Degree meter 6 is arranged at mark 0.5~0.8 times away from bottom height and position of case 1.
Invention device operating process
1 bottom of mark case is opened, the plant of experiment is put into mark case 1, the stem of labeled plant 20 is located at axis
Mouthful 14 positions, while install axis set 3, make mark case 1 be in it is completely closed in the case of；Filled by air inlet 8 into case
Enter carbon isotope (11CO2、13C and14C) experimental gas, is tested；In experimentation, at the same measure mark case in temperature,
Humidity, air pressure, CO2 concentration and illuminance.
Operating process controls
Temperature measuring and warning device 19 are when temperature exceedes 5 DEG C of outside air temperature, automatic alarm, and when alarm needs to change
Cold water, the cold water temperature of replacement is 5~15 DEG C, and cold water is prepared for the distillation water cooling that tap water is handled by distillation.
Temperature control box gas circulator 7 is opened 4~8 hours daily, and CO2 concentration and air pressure tester 5 should be in gas when measuring
7 operation 20min of body circulation pump is carried out later；Pressure reducing valve 9 mark case 1 in pressure be more than outside atmospheric pressure 5% when, decompression
Valve 9 is opened, pressure reducing valve unlatching before, gas circulator 7 answer out of service 5 it is small when more than；The measure of Hygrothermograph 4 should be per in the daytime
Measure when 2~4 is small, when humidity is less than outside humidity, be humidified by air inlet plus water；It is early daily when illumination photometer 6 measures, in
With evening respectively no less than once.
Embodiment of above is merely illustrative of the technical solution of the present invention, rather than its limitations, of the invention real not departing from
All any modification, equivalent and improvement made within the spirit and principle of matter etc., should be included in protection scope of the present invention
Within.
Claims (8)
- A kind of 1. carbon isotope labelling device of clone plant independence plant division, it is characterised in that：By mark case (1) and temperature control water tank (2) two large divisions is formed, and top is connected by outlet pipe (11) between mark case (1) and temperature control water tank (2), and lower part is by water inlet pipe (10) connect；Mark the side wall of case (1) that Hygrothermograph (4), CO2 concentration and air pressure tester (5), illumination photometer (6), bottom are set Gas circulator (7), axis mouth (14) and air inlet (8) are set, and using double-deck water-filling side wall (13), it is pushed up mark case (1) Portion sets pressure reducing valve (9), and the bottom of double-deck water-filling side wall (13) sets water outlet (17), entirely marks case (1) to be placed on buttress (12) on；The stem of labeled plant (20) is arranged at axis mouth (14) position, and axis set is set in axis mouth (14) (3), the interior set temperature measure of temperature control water tank (2) and warning device (19).
- 2. mark case (1) side wall according to claim 1 and top use transparent film or transparent glass preparation；Temperature control Distilled water is full of in water tank (2) and the double-deck water-filling side wall (13) of mark case (1), temperature control water tank (2) interior water is double-deck water-filling side 10~15 times of wall (13) interior water；Water inlet pipe (10) and outlet pipe (11) and the angle of horizontal direction are more than 30 degree, water inlet pipe (10) and outlet pipe (11) a diameter of 20~50mm, the vertical clearance of mark case (1) and temperature control water tank (2) is from for 0.5~3.0m.
- 3. temperature measuring according to claim 1 and warning device (19) are arranged at temperature control water tank (2) inner wall, its position is high Spend for 0.35~0.55 times of temperature control water tank (2) height；The top of water tank sets water inlet (18), its diameter or the length of side are 0.05 ~0.10m；Water outlet (17) diameter or the length of side of double-deck water-filling side wall (13) bottom are 0.04~0.08m.
- 4. with top to combine formation, groove, top and bottom are left in bottom for mark case (1) bottom according to claim 1 When portion combines, the bottom of double-deck water-filling side wall (13) is put into groove, while is sealed using fluid sealant；Mark the bottom of case (1) Portion is put together for two boards along split line (16), and mosaic is sealed using fluid sealant, while both ends use fixing piece (15) It is fixed.
- 5. the bottom plate air inlet (8) of mark case (1) according to claim 1 is self-seal device, mark case (1) bottom is set The gas circulator (7) put, both sides are arranged symmetrically, and are opened daily 4~8 hours, CO2 concentration and air pressure tester (5) measure When should gas circulator (7) run 20min after carry out.
- 6. temperature measuring according to claim 1 and warning device (19) be when temperature exceedes 5 DEG C of outside air temperature, from Dynamic alarm, when alarm, need to change cold water, and the cold water temperature of replacement is 5~15 DEG C, and cold water handles to obtain for tap water by distillation Distillation water cooling prepare.
- 7. pressure reducing valve (9) according to claim 1 subtracts when marking case (1) interior pressure to be more than the 5% of outside atmospheric pressure Pressure valve (9) open, pressure reducing valve unlatching before, gas circulator (7) answer out of service 5 it is small when more than.
- 8. Hygrothermograph (4) measure according to claim 1 should be measured when every day interval 2~4 is small, it is less than room in humidity During outer humidity, it is humidified by air inlet plus water；Illumination photometer (6) is arranged at mark case (1) 0.5~0.8 times away from bottom height and position, It measures early daily, neutralization evening respectively no less than once.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610932564.8A CN107969243A (en) | 2016-10-25 | 2016-10-25 | A kind of carbon isotope labelling device of clone plant independence plant division |
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| CN201610932564.8A CN107969243A (en) | 2016-10-25 | 2016-10-25 | A kind of carbon isotope labelling device of clone plant independence plant division |
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| CN201610932564.8A Pending CN107969243A (en) | 2016-10-25 | 2016-10-25 | A kind of carbon isotope labelling device of clone plant independence plant division |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801735A (en) * | 2018-06-26 | 2018-11-13 | 深圳雨伦环境科技有限公司 | A kind of plant leaf blade water isotope enrichment signal tagging system and labeling method |
| CN111751500A (en) * | 2020-07-06 | 2020-10-09 | 吉林建筑大学 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
| CN113960245A (en) * | 2021-09-02 | 2022-01-21 | 华东师范大学 | Approximate determination method for contribution of clone integration to internode formation of rhizome type or stolon type clone plant |
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| CN205631202U (en) * | 2016-03-30 | 2016-10-12 | 巴中市天成高分子材料有限公司 | Foaming machine of sponge |
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| CN201894089U (en) * | 2010-11-16 | 2011-07-13 | 香港城市大学深圳研究院 | Device for adopting stable carbon isotope gas for marking plants |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108801735A (en) * | 2018-06-26 | 2018-11-13 | 深圳雨伦环境科技有限公司 | A kind of plant leaf blade water isotope enrichment signal tagging system and labeling method |
| CN111751500A (en) * | 2020-07-06 | 2020-10-09 | 吉林建筑大学 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
| CN113960245A (en) * | 2021-09-02 | 2022-01-21 | 华东师范大学 | Approximate determination method for contribution of clone integration to internode formation of rhizome type or stolon type clone plant |
| CN113960245B (en) * | 2021-09-02 | 2024-03-26 | 华东师范大学 | Method for approximately determining contribution of clone integration to internode formation of rhizome type or creeping stem type clone plants |
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