CN110663387B - Plant material 13 C isotope labeling device - Google Patents
Plant material 13 C isotope labeling device Download PDFInfo
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- CN110663387B CN110663387B CN201911094415.9A CN201911094415A CN110663387B CN 110663387 B CN110663387 B CN 110663387B CN 201911094415 A CN201911094415 A CN 201911094415A CN 110663387 B CN110663387 B CN 110663387B
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- 239000000463 material Substances 0.000 title claims description 11
- 238000001948 isotopic labelling Methods 0.000 title abstract description 13
- 239000004698 Polyethylene Substances 0.000 claims abstract description 67
- -1 polyethylene Polymers 0.000 claims abstract description 67
- 229920000573 polyethylene Polymers 0.000 claims abstract description 67
- 230000001105 regulatory effect Effects 0.000 claims abstract description 14
- 239000007789 gas Substances 0.000 claims description 66
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000005406 washing Methods 0.000 claims description 17
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 15
- 229910052802 copper Inorganic materials 0.000 claims description 15
- 239000010949 copper Substances 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 238000004321 preservation Methods 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 2
- 238000004868 gas analysis Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 28
- 238000000034 method Methods 0.000 description 10
- 229910052799 carbon Inorganic materials 0.000 description 7
- 238000002372 labelling Methods 0.000 description 6
- 239000011229 interlayer Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- 241000219000 Populus Species 0.000 description 3
- 238000005485 electric heating Methods 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000004158 soil respiration Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012613 in situ experiment Methods 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3504—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing gases, e.g. multi-gas analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V15/00—Tags attached to, or associated with, an object, in order to enable detection of the object
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- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- General Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Geophysics (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a plant 13 C isotope marking device relates to isotope marking technical field. The device comprises a marking device, a blower, an infrared gas analyzer, a gas absorbing device, a thermometer, a pressure regulating device, a temperature control device and a gas generating device, wherein the marking device comprises a three-way pipe, a four-way pipe, a PVC pipe, a polyethylene sleeve bag and a polyethylene film, the three-way pipe and the four-way pipe are mutually inserted through the PVC pipe, the surfaces of the three-way pipe, the four-way pipe and the PVC pipe are sleeved with the inside of the polyethylene sleeve bag, and the polyethylene film is fixedly arranged at the bottom of the polyethylene sleeve bag. Through setting up three-way pipe, four-way pipe and PVC pipe, the height of marking device equals three meters, can be with the plant successful mark of two seven meters up to that field is planted, solved the problem that general marking device can only mark low potted plant, widened 13 Application range of C isotope labeling technology.
Description
Technical Field
The invention relates to the technical field of isotope labeling, in particular to a plant 13 C isotope labeling device.
Background
The carbon isotopes in nature include 12 C、 13 C and C 14 C, wherein 12 C、 13 C is a stable isotope of carbon and, 14 c is radioactive. The carbon isotope with highest abundance in the nature is 12 C, and 13 c and C 14 The abundance of C is very low. Because the stable isotope has the advantages of stable physical property, no ionizing radiation and the like, the stable isotope is stable 13 C isotope technology has been widely used to study the turnover of organic carbon in soil. In the prior art, the method comprises the steps of, 13 c isotope labeling includes natural labeling and artificial labeling. Natural markers are commonly used in field in situ experiments, require replacement of C3 and C4 plants, and require relatively long replacement times (in years); the artificial labeling can select a proper reagent, the labeling is performed under a fully controllable condition, and the target abundance can be artificially regulated. The manual marking is performed by using a proper marking device, and the existing marking device has the following problems: limited by material and relative volumeSmaller, only short crops such as rice and wheat can be marked, woody plants with higher heights cannot be marked; the device is limited by the structure of the device, and the device can only be used for marking potted plants and is not suitable for marking the plants in situ in the field. Therefore, in order to achieve field in-situ marking of woody plants, a need exists for a relatively large, relatively simple structure that is easy to assemble and transport 13 C isotope labeling device.
Disclosure of Invention
(one) solving the technical problems
In order to overcome the defects of the prior art, the invention provides a method for carrying out the method on woody plants with relatively high heights 13 The device for marking the C isotope solves the defect that the prior device can only mark plants (such as herbaceous plants) with relatively low height, and widens 13 Application of C isotope labeling technology.
(II) technical scheme
In order to achieve the purposes of the marking device with simple structure, convenient assembly and light material quality, the invention provides the following technical scheme: plant material 13 The C isotope marking device comprises a marking device, a blower, an infrared gas analyzer, a gas absorbing device, a thermometer, a pressure regulating device, a temperature control device and a gas generating device, wherein the marking device comprises a three-way pipe, a four-way pipe, a PVC pipe, a polyethylene sleeve bag and a polyethylene film, the three-way pipe and the four-way pipe are mutually spliced through the PVC pipe, the surfaces of the three-way pipe, the four-way pipe and the PVC pipe are sleeved with the inside of the polyethylene sleeve bag, and the polyethylene film is fixedly arranged at the bottom of the marking device so as to avoid CO generated by soil respiration 2 Discharging into a marking device;
the inside of the polyethylene bagging is fixedly provided with a blower, the output end of the blower is fixedly provided with an air outlet pipe, the outside of the polyethylene bagging is fixedly provided with an infrared gas analyzer (British ADC company, EGA61 multichannel soil respiration measuring system), the output end of the infrared gas analyzer is fixedly provided with a connecting pipe, and one end of the connecting pipe, which is far away from the infrared gas analyzer, extends to the inside of the polyethylene bagging;
the gas absorption device comprises a first air pump, wherein a first air pump is fixedly arranged in the polyethylene sleeve bag, a first hose is fixedly arranged at the output end of the first air pump, a gas washing bottle is fixedly arranged outside the polyethylene sleeve bag, one end of the first hose, which is far away from the first air pump, extends to the inside of the gas washing bottle, a second hose is fixedly arranged in the gas washing bottle, one end of the second hose, which is far away from the gas washing bottle, extends to the inside of the polyethylene sleeve bag, and a thermometer is fixedly arranged on the surface of the PVC pipe;
the pressure regulating device comprises a second air pump, the second air pump is fixedly arranged in the polyethylene bagging, the left end of the air bag conduit is fixedly provided with a first hose air bag conduit and a second hose air bag conduit through a Y-shaped tee joint, the output end of the second air pump is fixedly connected with one end, far away from the air bag conduit, of the first hose air bag conduit and the second hose air bag conduit, the valve is fixedly arranged in the first hose air bag conduit and the second hose air bag conduit, the medical oxygen bag is fixedly arranged outside the polyethylene bagging, the right end of the air bag conduit extends to the inside of the medical oxygen bag, and the surface of the PVC pipe is fixedly provided with an empty box pressure gauge.
As a preferable technical scheme of the invention, the number of the three-way pipes is four, every two three-way pipes are horizontally placed in a group of front and back, the number of the four-way pipes is four, every two three-way pipes are horizontally placed in a group of front and back, the four three-way pipes are respectively vertical to the four-way pipes in pairs, the opposite surfaces of the four three-way pipes are mutually spliced through PVC pipes, the opposite surfaces of the four-way pipes are mutually spliced through PVC pipes, the opposite surfaces of the three-way pipes and the four-way pipes are mutually spliced through PVC pipes, the bottoms of the four-way pipes are respectively spliced with PVC pipes, the height of the marking device is equal to three meters, and the length and the width of the marking device are all one meter.
As a preferable technical scheme of the invention, the inside of the gas washing bottle is filled with NaOH solution.
As a preferable technical scheme of the invention, a temperature control device is fixedly arranged outside the polyethylene bagging and comprises a water tank, a heat insulation barrel, a third air pump, a spiral red copper heat exchange tube and a conveying pipe, wherein water is arranged inside the water tank, the spiral red copper heat exchange tube is positioned below the water surface in the water tank, the heat insulation barrel wraps the whole water tank and is provided with an interlayer between the water tank and the water tank, the third air pump is positioned inside the polyethylene bagging, the output end of the third air pump extends to the inside of the water tank through the conveying pipe and is fixedly connected with the top of the spiral red copper heat exchange tube, the conveying pipe is fixedly arranged at the bottom of the spiral red copper heat exchange tube, one end of the conveying pipe, which is far away from the spiral red copper heat exchange tube, extends to the inside of the polyethylene bagging, an interlayer is arranged outside the water tank, and the heat insulation barrel is arranged outside the interlayer.
As an optimized technical scheme of the invention, a gas generating device is fixedly arranged outside the polyethylene bagging and comprises a wide-mouth bottle, a separating funnel, a first gas guide pipe, a second gas guide pipe, a third gas guide pipe and a first air pump, wherein a double-hole rubber plug is fixedly arranged inside the wide-mouth bottle, one end of the separating funnel is inserted into one hole of the double-hole rubber plug and extends to the inside of the wide-mouth bottle, the other hole of the double-hole rubber plug is fixedly provided with the first gas guide pipe, one end of the first gas guide pipe, which is far away from the wide-mouth bottle, is respectively communicated with the second gas guide pipe and the third gas guide pipe through a Y-shaped tee, one end of the second gas guide pipe, which is far away from the first gas guide pipe, extends to the inside of the polyethylene bagging, one end of the third gas guide pipe extends to the inside of the polyethylene bagging and is fixedly connected with the input end of the first air pump, the first air pump is positioned inside the polyethylene bagging, and valves which can be installed inside the second gas guide pipe and the third gas guide pipe and can only completely close the gas generating device.
(III) beneficial effects
Compared with the prior art, the invention provides a plant 13 The C isotope labeling device has the following beneficial effects:
1. the plant is provided 13 C isotope marking device is provided with a three-way pipe and four-way pipeThe height of the marking device is equal to three meters, so that plants which are planted in the field and reach two points and seven meters can be successfully marked, the problem that the general marking device can only mark low potted plants is solved, and the plants are widened 13 Application of C isotope labeling technology.
2. The plant is provided 13 The C isotope labeling device is light in material and convenient to transport, and solves the problems of heavy weight and difficult transportation of a conventional large-sized hard box body.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of a gas generator according to the present invention;
fig. 3 is a schematic structural diagram of a temperature control device according to the present invention.
In the figure: 1. a marking device; 11. a three-way pipe; 12. a four-way pipe; 13. a PVC pipe; 14. polyethylene bagging; 15. a polyethylene film; 2. a blower; 21. an air outlet pipe; 3. an infrared gas analyzer; 31. a connecting pipe; 4. a gas absorbing device; 41. a first air pump; 42. a first hose; 43. a gas washing bottle; 44. a second hose; 5. a thermometer; 6. a pressure regulating device; 61. a second air pump; 62. an air bag conduit; 621. a first hose air bag conduit; 622. a second hose air bag conduit; 63. medical oxygen bag; 64. an empty box pressure gauge; 7. a temperature control device; 71. a water tank; 72. a third air pump; 73. spiral red copper heat exchange tube; 74. a delivery tube; 75. an electric heating tube; 76. a heat-preserving barrel; 77. an interlayer; 8. a gas generating device; 81. a jar; 82. a separating funnel; 83. a first air duct; 84. a second air duct; 85. a third air duct; 86. a first suction pump.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to FIGS. 1-3, the present invention discloses a plant 13 The C isotope marking device comprises a marking device 1, a blower 2, an infrared gas analyzer 3, a gas absorbing device 4, a thermometer 5, a pressure regulating device 6, a temperature control device 7 and a gas generating device 8, wherein the marking device 1 comprises a three-way pipe 11, a four-way pipe 12, a PVC pipe 13, a polyethylene sleeve bag 14 and a polyethylene film 15, the three-way pipe 11 and the four-way pipe 12 are mutually spliced through the PVC pipe 13, the surfaces of the three-way pipe 11, the four-way pipe 12 and the PVC pipe 13 are sleeved with the inside of the polyethylene sleeve bag 14, and the polyethylene film 15 is fixedly arranged at the bottom of the marking device 1;
the inside of the polyethylene bagging 14 is fixedly provided with a blower 2, the model of the blower 2 is XGB-120, the output end of the blower 2 is fixedly provided with an air outlet pipe 21, the outside of the polyethylene bagging 14 is fixedly provided with an infrared gas analyzer 3, the model of the infrared gas analyzer 3 is EGA61, the output end of the infrared gas analyzer 3 is fixedly provided with a connecting pipe 31 (4X 6mm and made of silica gel), and one end of the connecting pipe 31 far away from the infrared gas analyzer 3 extends to the inside of the polyethylene bagging 14;
the gas absorption device 4 comprises a first air pump 41, wherein the first air pump 41 is fixedly arranged in the polyethylene bagging 14, a first hose 42 (6X 9mm and made of silica gel) is fixedly arranged at the output end of the first air pump 41, a gas washing bottle 43 is fixedly arranged outside the polyethylene bagging 14, one end of the first hose 42, which is far away from the first air pump 41, extends to the inside of the gas washing bottle 43, a second hose 44 is fixedly arranged inside the gas washing bottle 43, one end of the second hose 44, which is far away from the gas washing bottle 43, extends to the inside of the polyethylene bagging 14, a thermometer 5 is fixedly arranged on the surface of the PVC pipe 13, and the model number of the thermometer 5 is YHZ-90191;
the pressure regulating device 6 comprises a second air pump 61, the specification of the first air pump 41 is consistent with that of the second air pump 61, the model of the second air pump 61 is ACO-001, the second air pump 61 is fixedly arranged in the polyethylene sleeve bag 14, and the left end of the air bag conduit 62 is fixedly provided with a first hose air bag conduit 621 and a second hose air bag conduit 6 through Y-shaped tee joints22, the output end of the second air pump 61 is fixedly connected with one end of the first hose air bag conduit 621 far away from the air bag conduit 62, the inner parts of the first hose air bag conduit 621 and the second hose air bag conduit 622 are fixedly provided with valves, the medical oxygen bag 63 is fixedly arranged outside the polyethylene bagging 14, the right end of the air bag conduit 62 extends to the inner part of the medical oxygen bag 63, the surface of the PVC pipe 13 is fixedly provided with an empty box pressure gauge 64, the model of the empty box pressure gauge 64 is DYM3, by arranging the three-way pipe 11, the four-way pipe 12 and the PVC pipe 13, the height of the marking device 1 is equal to three meters, plants which are planted in the field and are up to two seven-point seven-meter can be successfully marked, the problem that the general marking device 1 can only mark low potted plants is solved, and the plants are widened 13 Use of C isotope labeling.
Specifically, the number of the three-way pipes 11 is four and the four-way pipes are horizontally placed around every two three-way pipes are a group, the number of the four-way pipes 12 is four and the four three-way pipes 11 are horizontally placed around every two three-way pipes are a group, the four three-way pipes 11 are respectively vertical to the four-way pipes 12 in pairs, the four opposite faces of the four-way pipes 11 are mutually spliced through PVC pipes 13, the four opposite faces of the four-way pipes 12 are mutually spliced through PVC pipes 13, the opposite faces of the three-way pipes 11 and the four-way pipes 12 are mutually spliced through PVC pipes 13, the four bottom ends of the four-way pipes 12 are respectively spliced with PVC pipes 13, the height of the marking device 1 is equal to three meters, and the length and the width of the marking device 1 are all one meter.
In the embodiment, the height of the marking device 1 is equal to three meters, so that plants which are planted in the field and reach two points and seven meters can be successfully marked, and the problem that the common marking device can only mark short potted plants is solved.
Specifically, the inside of the gas washing bottle 43 contains NaOH solution.
In this embodiment, the NaOH solution is effective in removing CO extracted into the gas wash bottle 43 2 The marking effect of the device is improved.
Specifically, the outside fixed mounting of polyethylene cover bag 14 has temperature regulating device 7, temperature regulating device 7 includes basin 71, third air pump 72 (XGB-250, zhejiang Jinye mechano-electric limited company), spiral red copper heat exchange tube 73 (19×1mm, red copper material), conveyer pipe 74 (19×2.5mm, PVC and steel wire composite material), electrothermal tube 75, heat preservation bucket 76 and intermediate layer 77, the inside of basin 71 holds water, install electrothermal tube 75 below the surface of water in the basin 71, third air pump 72 is located the inside of polyethylene cover bag 14, the output of third air pump 72 extends to the inside of basin 71 and with the top fixed connection of spiral red copper heat exchange tube 73 through conveyer pipe 74, the quantity of conveyer pipe 74 is two, the bottom fixed mounting of spiral red copper heat exchange tube 73 has another conveyer pipe 74 and this conveyer pipe 74 keeps away from the one end extension to the inside of polyethylene cover bag 14 of spiral red copper heat exchange tube, the outside of basin 71 is provided with intermediate layer 77, the outside of intermediate layer 77 is provided with the heat preservation bucket 76.
In this embodiment, the water is contained in the water tank 71, when the air temperature is low (e.g. winter), the electric heating tube 75 below the water surface in the water tank is turned on to heat so as to increase the water temperature, when the air temperature is high (e.g. summer), the electric heating tube 75 is turned off, the interlayer 77 between the heat preservation barrel 76 and the water tank 71 is filled with crushed ice, the temperature of the water in the water tank 71 is adjusted according to the actual air temperature, and the temperature control device 7 is matched with the thermometer 5 so as to effectively adjust the temperature in the polyethylene bagging 14.
Specifically, the outside fixed mounting of polyethylene cover bag 14 has gas generator 8, gas generator 8 includes wide-mouth bottle 81, separating funnel 82, first air duct 83 (3 x 7mm, GG-17 borosilicate glass material), second air duct 84 (6 x 9mm, silica gel material), third air duct 85 (6 x 9mm, silica gel material) and first aspiration pump 86, the bottleneck department fixed mounting of wide-mouth bottle 81 has the diplopore rubber stopper, separating funnel 82 inserts a hole of diplopore rubber stopper and its lower part glass pipe extends to the inside of wide-mouth bottle 81, another hole fixed mounting of diplopore rubber stopper has first air duct 83, the one end that wide-mouth bottle 81 was kept away from to first air duct 83 is linked together with second air duct 84 and third air duct 85 respectively through Y type tee bend, the one end that first air duct 83 was kept away from to second air duct 84 extends to the inside of polyethylene cover bag 14, the one end that first air duct 83 was kept away from to third air duct 85 extends to the inside of polyethylene cover bag 14 and the inside of polyethylene cover 86 is located the pump 85, the inside is located the fixed air duct of 13. In this embodiment, the valve on the second air duct 84 is opened at the beginning, the valve on the third air duct 85 is closed, the gas is allowed to freely enter the polyethylene bagging 14, the valve on the third air duct 85 is opened near the end of the reaction, the valve on the second air duct 84 is closed, the first air pump 86 is opened to form negative pressure at the first air duct 83, meanwhile, a proper amount of water can be added into the wide-mouth bottle 81 through the separating funnel 82, and the gas in the wide-mouth bottle 81 is compressed, so that the gas in the gas generating device 8 enters the polyethylene bagging 14 as much as possible.
The working principle and the using flow of the invention are as follows: first, CO in the marking device 1 is made to pass through the gas absorbing device 4 2 The concentration was lowered, the first hose 42 and the second hose 44 were then closed, the gas-washing bottle 43 was removed, and the gas-generating apparatus 8 was constituted by using a separating funnel 82, a double-hole rubber stopper, a wide-mouth bottle 81, a first gas-guide tube 83, a Y-type tee, a second gas-guide tube 84, a third gas-guide tube 85 and a first suction pump 86, and then 125ml of 2 mol.L -1 Is added to a solution of 12.50g Ba 13 CO 3 And passing the generated gas into the marking device 1, thereby causing 13 CO 2 Up to a concentration of about 516. Mu.L.L -1 Based on the CO detected by EGA61 2 The labeling time was determined to be 1 hour and the labeling process was repeated 1 time for improvement 13 C assimilation efficiency Using unlabeled Ba 12 CO 3 Replacement of Ba 13 CO 3 The same operation is carried out, and repeated for 1 time, all devices are removed after marking is finished, poplar seedlings are taken out from soil, and the poplar seedlings are obtained 13 C-labeled poplar.
In conclusion, the plant 13 C isotope marking device, through setting up three-way pipe 11, four-way pipe 12 and PVC pipe 13, marking device 1 highly equals three meters, can be with the plant successful mark of up to 2.7m that plants planted in the field, solved the problem that general marking device can only mark low potted plant, widened the plant 13 Use of C isotope labeling.
It should be noted that in this document, terms such as "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. Plant material 13 C isotope marking device, including marking device (1), air-blower (2), infrared gas analysis appearance (3), gas absorption device (4), thermometer (5), pressure regulating device (6), temperature regulating device (7) and gas generating device (8), its characterized in that: the marking device (1) comprises a three-way pipe (11), a four-way pipe (12), a PVC pipe (13), a polyethylene sleeve bag (14) and a polyethylene film (15), wherein the three-way pipe (11) and the four-way pipe (12) are mutually spliced through the PVC pipe (13), the surfaces of the three-way pipe (11), the four-way pipe (12) and the PVC pipe (13) are sleeved with the inside of the polyethylene sleeve bag (14), and the polyethylene film (15) is fixedly arranged at the bottom of the marking device (1);
the inside of the polyethylene bagging (14) is fixedly provided with a blower (2), the output end of the blower (2) is fixedly provided with an air outlet pipe (21), the outside of the polyethylene bagging (14) is fixedly provided with an infrared gas analyzer (3), the output end of the infrared gas analyzer (3) is fixedly provided with a connecting pipe (31), and the input end of the connecting pipe (31) far away from the infrared gas analyzer (3) extends to the inside of the polyethylene bagging (14);
the gas absorption device (4) comprises a first air pump (41), the first air pump (41) is fixedly arranged in the polyethylene bagging (14), a first hose (42) is fixedly arranged at the output end of the first air pump (41), a gas washing bottle (43) is fixedly arranged outside the polyethylene bagging (14), one end, far away from the first air pump (41), of the first hose (42) extends to the inside of the gas washing bottle (43), a second hose (44) is fixedly arranged in the gas washing bottle (43), one end, far away from the gas washing bottle (43), of the second hose (44) extends to the inside of the polyethylene bagging (14), and a thermometer (5) is fixedly arranged on the surface of the PVC pipe (13);
the pressure regulating device (6) comprises a second air pump (61), the second air pump (61) is fixedly arranged in the polyethylene sleeve bag (14), a first hose air bag pipe (621) and a second hose air bag pipe (622) are fixedly arranged at the left end of the air bag pipe (62) through a Y-shaped tee joint, the output end of the second air pump (61) is fixedly connected with one end, far away from the air bag pipe (62), of the first hose air bag pipe (621) and one end, far away from the air bag pipe (62), of the second hose air bag pipe (622) are fixedly provided with valves, a medical oxygen bag (63) is fixedly arranged outside the polyethylene sleeve bag (14), the right end of the air bag pipe (62) extends to the inside of the medical oxygen bag (63), and an empty box pressure gauge (64) is fixedly arranged on the surface of the PVC pipe (13);
the three-way pipes (11) are four in number and are horizontally placed in a group of two front and back, the four-way pipes (12) are four in number and are horizontally placed in a group of two front and back, the four three-way pipes (11) are respectively perpendicular to the four-way pipes (12) in pairs, the opposite surfaces of the four three-way pipes (11) are mutually spliced through PVC pipes (13), the opposite surfaces of the four-way pipes (12) are mutually spliced through the PVC pipes (13), the opposite surfaces of the three-way pipes (11) and the four-way pipes (12) are mutually spliced through the PVC pipes (13), the bottoms of the four-way pipes (12) are respectively spliced with the PVC pipes (13), the height of the marking device (1) is equal to three meters, and the length and the width of the marking device are one meter;
the inside of the gas washing bottle (43) is filled with NaOH solution.
2. According to claimA plant as claimed in claim 1 13 C isotope marking device, its characterized in that: the outside fixed mounting of polyethylene cover bag (14) has temperature regulating device (7), temperature regulating device (7) include basin (71), third air pump (72), spiral red copper heat exchange tube (73), conveyer pipe (74), electrothermal tube (75), heat preservation bucket (76) and intermediate layer (77), the inside flourishing water of basin (71), install electrothermal tube (75) below the surface of water of basin (71), third air pump (72) are located inside polyethylene cover bag (14), the output of third air pump (72) extends to the inside top fixed connection of basin (71) and with spiral red copper heat exchange tube (73) through conveyer pipe (74), the quantity of conveyer pipe (74) is two, the bottom fixed mounting of spiral red copper heat exchange tube (73) has another conveyer pipe (74) and this conveyer pipe (74) are kept away from the one end of spiral red copper heat exchange tube and are extended to the inside of polyethylene cover bag (14), the outside of basin (71) is provided with intermediate layer (77), the outside of intermediate layer (76) are provided with heat preservation bucket.
3. A plant according to claim 1 13 C isotope marking device, its characterized in that: the outside fixed mounting of polyethylene cover bag (14) has gas generating device (8), gas generating device (8) include wide-mouth bottle (81), separating funnel (82), first air duct (83), second air duct (84), third air duct (85) and first aspiration pump (86), the bottleneck department fixed mounting of wide-mouth bottle (81) has the diplopore rubber stopper, separating funnel (82) inserts one hole and lower part glass pipe extension to the inside of wide-mouth bottle (81) of diplopore rubber stopper, another hole fixed mounting of diplopore rubber stopper has first air duct (83), the one end that first air duct (83) kept away from wide-mouth bottle (81) is linked together with second air duct (84) and third air duct (85) respectively through Y tee bend, the one end that first air duct (83) was kept away from to second air duct (84) extends to the inside of polyethylene cover bag (14), third air duct (85) is kept away from the inside of first air duct (83) and polyethylene cover (86) and is located the inside of first air duct (14) and the air pump (84), the one end that first air duct (84) was kept away from first air duct (83) extends to the inside of polyethylene cover bag (14), the air pump (14) is located inside of third air duct (84)Valves are arranged in the air pipes (85).
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| CN201911094415.9A CN110663387B (en) | 2019-11-11 | 2019-11-11 | Plant material 13 C isotope labeling device |
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| CN207380011U (en) * | 2017-11-06 | 2018-05-18 | 中国矿业大学(北京) | A kind of device for detecting crop rhizosphere breathing and plant carbon markings |
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